Rorug04G0297000

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002146
Physical Location & Seq
Forward (+)
47169900 .. 47171128
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0297000.1

Sequence Viewer

Length: 1020 bp
ATGGGAGCAGTACATTTGGTAAAGGATCTAATAACTGCTACCGGTTATTGGGATGTGCCGCTTCTTCATCAGTTGTTCACATTGGAGGAGGTGGAGGTTATTTTGCGGATTCCATTGAGTAACCGAGCTGTGGAGGATCGTTTAGTTTGGAAGTTGGCTAAGGATGGTCAATTCTCAGTCAAGTCTACTTATAATCATTCGGTGCAGGCCGATCCTAATATAGTTCCGTTTGATCTTCCTGTGAATGAGCGGTTTTGGCAGAAGCTTTGGAGGGTGATGTTCCGAATTCAGCCAAGATATATATTTGGAAGATGGTTACAGTTCTGTGTGCAGGAACTTTCATTACAAGATTTGGGGATCCTAATTTGCTTGCTCTGGGAGATTTGGAGGGAGAGAAACCACAGAGTGTGGGAACAAAAGAAGAGTGCAGCTTGTGATGTCGTTTTGAAAGCTATGTCACGCTTACAAGAGTTTGGTTTCCACAACTCAAAACCGGTTAATTCGAATGCTAGAAGGTCCAGAGGAGTGAGGTGGTCTGCTCCACCAGTAGGAGTCACTAAAGTGAATGTGGACGGGGCATTTAATCTTACTACCAGAAAAGGAGGGATAGGTTTTGTCGTCCGGAACTCGGAGGGACAAATGTTAGCTAGTGGTGGCCGACCTTTATCCGGATTGGTATCAGCGGAGCATGCTGAATTATTGGCCTGTCGAATGGCTATTGAATTCATACTGCAACAGGGGATTAGTCCTGCAGTAGTGGAGACTGATGCATTGGTGGTGCAACGTCAAATTGTGGAGCATGGTGGGAATAATACATCGGTTCTGGGTCGTTTATATGAAGATTTGGCTGCATGTTTGGCAGCACATACAGATTTATCGATTACGTACAAGGGTAGAGCGGCTAATATGGCAGCTCATACTTTGGCTGCACATGCGTGTGCTGCATCTCAAGTTTTTTTCTTTTTTTCTACTCCGTCTTTTCTTCAAGCTGCTATTGCAGCTGATCTTTCATTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

339

Amino Acids

37.88

Weight (kDa)

8.92

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 189 - 312 2e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 192
AccBSI CCGCTC 2 cut(s) 250, 899
AccI GTMKAC 1 cut(s) 185
AccIII TCCGGA 2 cut(s) 621, 668
AciI CCGC 5 cut(s) 59, 106, 250, 683, 899
AclWI GGATC 5 cut(s) 33, 144, 206, 352, 365
AcoI YGGCCR 1 cut(s) 655
AcsI RAATTY 2 cut(s) 285, 722
AdeI CACNNNGTG 1 cut(s) 406
AfaI GTAC 2 cut(s) 12, 887
AfiI CCNNNNNNNGG 5 cut(s) 48, 130, 548, 628, 668
AgeI ACCGGT 2 cut(s) 41, 493
AgsI TTSAA 3 cut(s) 448, 722, 986
AleI CACNNNNGTG 2 cut(s) 560, 934
AluBI AGCT 8 cut(s) 128, 265, 431, 452, 647, 914, 989, 1001
AluI AGCT 8 cut(s) 128, 265, 431, 452, 647, 914, 989, 1001
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 5 cut(s) 33, 144, 206, 352, 365
Aor13HI TCCGGA 2 cut(s) 621, 668
AoxI GGCC 3 cut(s) 207, 655, 702
ApeKI GCWGC 8 cut(s) 428, 848, 860, 911, 926, 941, 989, 998
ApoI RAATTY 2 cut(s) 285, 722
AsiGI ACCGGT 2 cut(s) 41, 493
AspS9I GGNCC 1 cut(s) 516
AsuHPI GGTGA 1 cut(s) 286
AsuII TTCGAA 1 cut(s) 503
AvaII GGWCC 1 cut(s) 516
BamHI GGATCC 1 cut(s) 357
BbvI GCAGC 8 cut(s) 440, 835, 872, 913, 923, 928, 976, 1010
BccI CCATC 2 cut(s) 158, 306
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 2 cut(s) 510, 648
BfmI CTRYAG 1 cut(s) 750
Bme18I GGWCC 1 cut(s) 516
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 1 cut(s) 359
BmsI GCATC 2 cut(s) 757, 953
Bpu10I CCTNAGC 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 933
Bsa29I ATCGAT 1 cut(s) 878
BsaAI YACGTR 1 cut(s) 885
BsaBI GATNNNNATC 1 cut(s) 676
BsaWI WCCGGW 4 cut(s) 41, 493, 621, 668
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 130, 548, 628, 668
Bse118I RCCGGY 2 cut(s) 41, 493
Bse1I ACTGG 1 cut(s) 545
Bse8I GATNNNNATC 1 cut(s) 676
BseAI TCCGGA 2 cut(s) 621, 668
BseCI ATCGAT 1 cut(s) 878
BseGI GGATG 2 cut(s) 58, 169
BseJI GATNNNNATC 1 cut(s) 676
BseLI CCNNNNNNNGG 5 cut(s) 48, 130, 548, 628, 668
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 545
BseRI GAGGAG 2 cut(s) 101, 537
BseXI GCAGC 8 cut(s) 440, 835, 872, 913, 923, 928, 976, 1010
BsgI GTGCAG 4 cut(s) 224, 350, 447, 912
BshFI GGCC 3 cut(s) 209, 657, 704
BshTI ACCGGT 2 cut(s) 41, 493
BshVI ATCGAT 1 cut(s) 878
BsiSI CCGG 4 cut(s) 42, 494, 622, 669
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 5 cut(s) 48, 130, 548, 628, 668
BsmAI GTCTC 1 cut(s) 755
BsmFI GGGAC 1 cut(s) 648
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 3 cut(s) 209, 657, 704
Bsp119I TTCGAA 1 cut(s) 503
Bsp13I TCCGGA 2 cut(s) 621, 668
Bsp143I GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
BspACI CCGC 5 cut(s) 59, 106, 250, 683, 899
BspANI GGCC 3 cut(s) 209, 657, 704
BspCNI CTCAG 1 cut(s) 188
BspDI ATCGAT 1 cut(s) 878
BspEI TCCGGA 2 cut(s) 621, 668
BspLI GGNNCC 1 cut(s) 359
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 5 cut(s) 33, 144, 206, 352, 365
BspT104I TTCGAA 1 cut(s) 503
BsrBI CCGCTC 2 cut(s) 250, 899
BsrFI RCCGGY 2 cut(s) 41, 493
BsrI ACTGG 1 cut(s) 545
BssAI RCCGGY 2 cut(s) 41, 493
BssMI GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
Bst4CI ACNGT 1 cut(s) 321
Bst6I CTCTTC 1 cut(s) 416
BstBAI YACGTR 1 cut(s) 885
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 207, 371, 690
BstDEI CTNAG 2 cut(s) 159, 175
BstF5I GGATG 2 cut(s) 58, 169
BstKTI GATC 6 cut(s) 28, 139, 214, 235, 360, 1006
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
BstMWI GCNNNNNNNGC 8 cut(s) 256, 689, 857, 908, 932, 941, 995, 998
BstNSI RCATGY 3 cut(s) 692, 855, 935
BstSFI CTRYAG 1 cut(s) 750
BstSNI TACGTA 1 cut(s) 885
BstV1I GCAGC 8 cut(s) 440, 835, 872, 913, 923, 928, 976, 1010
BstX2I RGATCY 2 cut(s) 25, 357
BstYI RGATCY 2 cut(s) 25, 357
Bsu15I ATCGAT 1 cut(s) 878
BsuRI GGCC 3 cut(s) 209, 657, 704
BsuTUI ATCGAT 1 cut(s) 878
BtsCI GGATG 2 cut(s) 58, 169
Cac8I GCNNGC 3 cut(s) 207, 371, 690
Cfr10I RCCGGY 2 cut(s) 41, 493
Cfr13I GGNCC 1 cut(s) 516
ClaI ATCGAT 1 cut(s) 878
Csp6I GTAC 2 cut(s) 11, 886
CspAI ACCGGT 2 cut(s) 41, 493
CviAII CATG 4 cut(s) 689, 800, 852, 932
CviQI GTAC 2 cut(s) 11, 886
DdeI CTNAG 2 cut(s) 159, 175
DpnI GATC 6 cut(s) 27, 138, 213, 234, 359, 1005
DpnII GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
DraIII CACNNNGTG 1 cut(s) 406
EaeI YGGCCR 1 cut(s) 655
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Eco105I TACGTA 1 cut(s) 885
Eco47I GGWCC 1 cut(s) 516
EcoRI GAATTC 2 cut(s) 285, 722
EcoT22I ATGCAT 1 cut(s) 772
FaeI CATG 4 cut(s) 692, 803, 855, 935
FaqI GGGAC 1 cut(s) 648
FatI CATG 4 cut(s) 688, 799, 851, 931
FblI GTMKAC 1 cut(s) 185
FokI GGATG 2 cut(s) 65, 176
FspBI CTAG 2 cut(s) 510, 648
HaeIII GGCC 3 cut(s) 209, 657, 704
HapII CCGG 4 cut(s) 42, 494, 622, 669
Hin1II CATG 4 cut(s) 692, 803, 855, 935
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 2 cut(s) 109, 552
HpaII CCGG 4 cut(s) 42, 494, 622, 669
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 3 cut(s) 78, 186, 571
Hpy188I TCNGA 2 cut(s) 284, 631
Hpy188III TCNNGA 3 cut(s) 519, 622, 669
Hpy8I GTNNAC 3 cut(s) 78, 186, 571
HpyAV CCTTC 1 cut(s) 507
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4IV ACGT 2 cut(s) 784, 884
HpyF10VI GCNNNNNNNGC 8 cut(s) 256, 689, 857, 908, 932, 941, 995, 998
HpyF3I CTNAG 2 cut(s) 159, 175
HpySE526I ACGT 2 cut(s) 784, 884
Hsp92II CATG 4 cut(s) 692, 803, 855, 935
Kpn2I TCCGGA 2 cut(s) 621, 668
Kzo9I GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
LmnI GCTCC 4 cut(s) 5, 544, 685, 796
Lsp1109I GCAGC 8 cut(s) 440, 835, 872, 913, 923, 928, 976, 1010
LweI GCATC 2 cut(s) 757, 953
MaeI CTAG 2 cut(s) 510, 648
MaeII ACGT 2 cut(s) 784, 884
MaeIII GTNAC 4 cut(s) 119, 315, 456, 553
MalI GATC 6 cut(s) 27, 138, 213, 234, 359, 1005
MbiI CCGCTC 2 cut(s) 250, 899
MboI GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
MboII GAAGA 6 cut(s) 56, 227, 321, 433, 851, 974
MflI RGATCY 2 cut(s) 25, 357
MluCI AATT 7 cut(s) 170, 285, 363, 499, 695, 722, 789
MlyI GAGTC 1 cut(s) 561
Mph1103I ATGCAT 1 cut(s) 772
MroI TCCGGA 2 cut(s) 621, 668
MseI TTAA 2 cut(s) 498, 582
MslI CAYNNNNRTG 3 cut(s) 560, 934, 936
MspA1I CMGCKG 2 cut(s) 683, 1001
MspI CCGG 4 cut(s) 42, 494, 622, 669
Mva1269I GAATGC 1 cut(s) 511
MwoI GCNNNNNNNGC 8 cut(s) 256, 689, 857, 908, 932, 941, 995, 998
NdeII GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
NlaIII CATG 4 cut(s) 692, 803, 855, 935
NlaIV GGNNCC 1 cut(s) 359
NmuCI GTSAC 2 cut(s) 456, 553
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 3 cut(s) 692, 855, 935
NspV TTCGAA 1 cut(s) 503
OliI CACNNNNGTG 2 cut(s) 560, 934
PaeI GCATGC 1 cut(s) 692
PctI GAATGC 1 cut(s) 511
PfeI GAWTC 1 cut(s) 109
PinAI ACCGGT 2 cut(s) 41, 493
PleI GAGTC 1 cut(s) 560
PpsI GAGTC 1 cut(s) 560
Ppu21I YACGTR 1 cut(s) 885
PsiI TTATAA 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 359
PspPI GGNCC 1 cut(s) 516
PsrI GAACNNNNNNTAC 2 cut(s) 327, 359
PstI CTGCAG 1 cut(s) 754
PsuI RGATCY 2 cut(s) 25, 357
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 2 cut(s) 12, 887
RsaNI GTAC 2 cut(s) 11, 886
RseI CAYNNNNRTG 3 cut(s) 560, 934, 936
SaqAI TTAA 2 cut(s) 498, 582
Sau3AI GATC 6 cut(s) 25, 136, 211, 232, 357, 1003
Sau96I GGNCC 1 cut(s) 516
SchI GAGTC 1 cut(s) 561
SfaNI GCATC 2 cut(s) 757, 953
SfcI CTRYAG 1 cut(s) 750
SfuI TTCGAA 1 cut(s) 503
SinI GGWCC 1 cut(s) 516
SmiMI CAYNNNNRTG 3 cut(s) 560, 934, 936
SmlI CTYRAG 1 cut(s) 948
SmoI CTYRAG 1 cut(s) 948
SnaBI TACGTA 1 cut(s) 885
SphI GCATGC 1 cut(s) 692
Sse9I AATT 7 cut(s) 170, 285, 363, 499, 695, 722, 789
SsiI CCGC 5 cut(s) 59, 106, 250, 683, 899
SspMI CTAG 2 cut(s) 510, 648
TaaI ACNGT 1 cut(s) 321
TaiI ACGT 2 cut(s) 787, 887
TaqI TCGA 3 cut(s) 503, 709, 878
TasI AATT 7 cut(s) 170, 285, 363, 499, 695, 722, 789
TatI WGTACW 1 cut(s) 10
TauI GCSGC 2 cut(s) 61, 902
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 498, 582
Tru9I TTAA 2 cut(s) 498, 582
TseFI GTSAC 2 cut(s) 456, 553
TseI GCWGC 8 cut(s) 428, 848, 860, 911, 926, 941, 989, 998
Tsp45I GTSAC 2 cut(s) 456, 553
TspDTI ATGAA 5 cut(s) 56, 330, 715, 852, 999
TspGWI ACGGA 2 cut(s) 216, 963
VpaK11BI GGWCC 1 cut(s) 516
XapI RAATTY 2 cut(s) 285, 722
XceI RCATGY 3 cut(s) 692, 855, 935
XmiI GTMKAC 1 cut(s) 185
XspI CTAG 2 cut(s) 510, 648
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.