Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002146

Physical Location & Seq

Genomic Coordinates Forward (+)

26891729 .. 26894123

Gene Structure 2395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1196500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1212 bp

ATGGGCTCCAGCTTGGTTGTTCTATTTGTTTGCTTAGTGTTTTTGCTAGTGTCACTTCCCAGAGTTCTAGCTCAGCAAACATTTTTCGATGTGACTAAATACGGTGGCGTTGGTGATGGAATAACTGATAATGTCAAGGCTTTCACCGATGCATGGACTGTAGCTTGCCAAAATAATGGGGGTGGTGTGGTCTTATTCCCACCCGGAGTATACTTGCTGAGCCCGTTGGTTTTGAACGGTAAATGCAAAGGACCAATCGGAGTTCAGATAGAGGGGACTCTACTGGCGCGGGTGGAGAAGGAATATTCGGTTGATATTGACCATTGGATCACCTTCAAATATGTTGACAACTTCGTAATCAACGGTGGTGGTTCGTTGAATGGGCAAGGACCTTCTGCTTGGCCCTATAACGACTGCAAGAAAAATCCACATTGCAAACGACTTCCTGGTAGTTTGAGATTGGATTACGTCACAAATGCAAGGATAAATCACATAACATCGATCAACAGCAAGAACCTCCACATCGGCCTTTTCGCAAGCCACGACATAACAATTAGCAGCATCAACATATCAGCCCCCGCTAACAGCCCCAACACCGACGGAATCCACCTCGGTTTGTCACAAAACATCCAAATCTTGGACGCAGCGATCGCCACCGGCGATGACTGTATAAGCTTCAGCACTGGAACAAACAACGTCAACATCAGCGGCGTTCAATGTGGTCCGGGCCACGGAATAAGCATCGGAAGCCTTGGCAAGATAGTTGGTGACAATGTTTCTTTTGTGGACATAAGAAACTGCACCTTCTTTGGTACTCAAAATGGAGTGAGGATCAAAACATGGGCTCCCTCTGAACCAGGCATTGTGTCACATATTACATTCGAGCAAATTCAAGTCGATCAGGTCGAAAACCCCGTCATCATTGATCAACAATATTGCCCTTCTCGTATTTGCGATCCAAAGGTCTACTCCCGAGTTCAAATCGAAGAAGTTCAGTTCAGTAACATTTGGGGGACTTCAATCACACAAAATGTAGTTAATCTTAAATGTAGCAAAACTACACCCTGTCAGAAGATTGGGTTGGATGACATCAACCTAACTTACAGCGGTCGTGAGGGACCGGCTCAATCAACATGTTCAAACGCAGATGTCAGAATTTCTGGCCGGCAAAATCCCCCCGCTTGCACAGGCAAAGATAAAGCTTTAGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

404

Amino Acids

43.26

Weight (kDa)

6.44

Isoelectric Point (pI)

30.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	28 - 77	1.8e-06	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	56 - 385	9e-91	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002146)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g12491 FvH4_1g12500 FvH4_3g23740 FvH4_4g28770
malus_domestica	MD03G1196500.v1.1 MD11G1207000.v1.1
prunus_persica	Prupe.4G218900_v2.0.a1 Prupe.4G228600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0100401 RchiOBHm_Chr4g0436881 RchiOBHm_Chr5g0041901 RchiOBHm_Chr5g0041941
rosa_laevigata	RLG00000006489 RLG00000016970 RLG00000016994 RLG00000034104
rosa_multiflora	Rmu_sc0001095.1_g000011 Rmu_sc0001464.1_g000018 Rmu_sc0009443.1_g000011 Rmu_sc0010997.1_g000006
rosa_roxburghii	Rroxscaffold_2G00142280 Rroxscaffold_5G00377950
rosa_rugosa	Rorug02G0087800 Rorug04G0297000 Rorug05G0197100
rosa_samantha	Rh2AG137300 Rh2AG137500 Rh2BG140500 Rh2BG140700 Rh2CG142600 Rh2DG142500 Rh4AG351300 Rh4BG360300 Rh4CG374600 Rh4DG354600 Rh5AG281700 Rh5AG281900 Rh5BG287500 Rh5CG318800 Rh5DG296100 Rh5DG296400
rosa_wichuraiana	Rw0G001350 Rw2G010740 Rw4G030760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	637
AccI	GTMKAC	2 cut(s)	210, 966
AccII	CGCG	1 cut(s)	289
AciI	CCGC	5 cut(s)	289, 579, 708, 1107, 1179
AclWI	GGATC	3 cut(s)	335, 839, 950
AcoI	YGGCCR	1 cut(s)	1162
AcsI	RAATTY	2 cut(s)	888, 1155
AcuI	CTGAAG	1 cut(s)	661
AfaI	GTAC	1 cut(s)	814
AfiI	CCNNNNNNNGG	3 cut(s)	153, 637, 731
AflIII	ACRYGT	1 cut(s)	1133
AgsI	TTSAA	8 cut(s)	235, 337, 379, 716, 893, 980, 1020, 1140
AjnI	CCWGG	2 cut(s)	445, 856
AjuI	GAANNNNNNNTTGG	2 cut(s)	1064, 1096
AluBI	AGCT	5 cut(s)	12, 71, 164, 675, 1202
AluI	AGCT	5 cut(s)	12, 71, 164, 675, 1202
AlwI	GGATC	3 cut(s)	335, 839, 950
Ama87I	CYCGRG	1 cut(s)	972
AoxI	GGCC	4 cut(s)	401, 526, 727, 1162
ApeKI	GCWGC	2 cut(s)	558, 644
ApoI	RAATTY	2 cut(s)	888, 1155
ArsI	GACNNNNNNTTYG	2 cut(s)	900, 932
AsiSI	GCGATCGC	1 cut(s)	651
Asp700I	GAANNNNTTC	1 cut(s)	990
AspLEI	GCGC	1 cut(s)	289
AspS9I	GGNCC	6 cut(s)	251, 389, 402, 722, 727, 1118
AsuC2I	CCSGG	2 cut(s)	204, 726
AsuHPI	GGTGA	4 cut(s)	125, 136, 322, 779
AvaI	CYCGRG	1 cut(s)	972
AvaII	GGWCC	4 cut(s)	251, 389, 722, 1118
BanII	GRGCYC	3 cut(s)	8, 224, 847
BbvI	GCAGC	2 cut(s)	570, 656
BccI	CCATC	1 cut(s)	110
BciT130I	CCWGG	2 cut(s)	447, 858
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	2 cut(s)	204, 726
BfaI	CTAG	2 cut(s)	47, 68
BfmI	CTRYAG	1 cut(s)	159
BisI	GCNGC	3 cut(s)	559, 645, 709
BlpI	GCTNAGC	2 cut(s)	72, 218
BlsI	GCNGC	3 cut(s)	560, 646, 710
Bme1390I	CCNGG	4 cut(s)	204, 447, 726, 858
Bme18I	GGWCC	4 cut(s)	251, 389, 722, 1118
BmeT110I	CYCGRG	1 cut(s)	972
BmgT120I	GGNCC	6 cut(s)	251, 389, 402, 722, 727, 1118
BmiI	GGNNCC	3 cut(s)	7, 846, 1119
BmrFI	CCNGG	4 cut(s)	204, 447, 726, 858
BmsI	GCATC	3 cut(s)	139, 570, 750
Bpu1102I	GCTNAGC	2 cut(s)	72, 218
BpuMI	CCSGG	2 cut(s)	204, 726
Bsa29I	ATCGAT	1 cut(s)	500
BsaJI	CCNNGG	3 cut(s)	610, 730, 751
BsaXI	ACNNNNNCTCC	2 cut(s)	829, 859
Bsc4I	CCNNNNNNNGG	3 cut(s)	153, 637, 731
Bse118I	RCCGGY	3 cut(s)	656, 1120, 1164
Bse1I	ACTGG	2 cut(s)	288, 688
Bse3DI	GCAATG	1 cut(s)	430
BseBI	CCWGG	2 cut(s)	447, 858
BseCI	ATCGAT	1 cut(s)	500
BseDI	CCNNGG	3 cut(s)	610, 730, 751
BseGI	GGATG	2 cut(s)	627, 1090
BseLI	CCNNNNNNNGG	3 cut(s)	153, 637, 731
BseMI	GCAATG	1 cut(s)	430
BseMII	CTCAG	2 cut(s)	86, 209
BseNI	ACTGG	2 cut(s)	288, 688
BseXI	GCAGC	2 cut(s)	570, 656
BsgI	GTGCAG	1 cut(s)	784
Bsh1236I	CGCG	1 cut(s)	289
Bsh1285I	CGRYCG	2 cut(s)	651, 1111
BshFI	GGCC	4 cut(s)	403, 528, 729, 1164
BshVI	ATCGAT	1 cut(s)	500
BsiEI	CGRYCG	2 cut(s)	651, 1111
BsiHKCI	CYCGRG	1 cut(s)	972
BsiSI	CCGG	5 cut(s)	204, 657, 725, 1121, 1165
BslFI	GGGAC	3 cut(s)	289, 1027, 1131
BslI	CCNNNNNNNGG	3 cut(s)	153, 637, 731
BsmFI	GGGAC	3 cut(s)	289, 1027, 1131
BsnI	GGCC	4 cut(s)	403, 528, 729, 1164
BsoBI	CYCGRG	1 cut(s)	972
Bsp1286I	GDGCHC	3 cut(s)	8, 224, 847
Bsp143I	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
Bsp1720I	GCTNAGC	2 cut(s)	72, 218
BspACI	CCGC	5 cut(s)	289, 579, 708, 1107, 1179
BspANI	GGCC	4 cut(s)	403, 528, 729, 1164
BspCNI	CTCAG	2 cut(s)	85, 210
BspDI	ATCGAT	1 cut(s)	500
BspFNI	CGCG	1 cut(s)	289
BspLI	GGNNCC	3 cut(s)	7, 846, 1119
BspPI	GGATC	3 cut(s)	335, 839, 950
BsrDI	GCAATG	1 cut(s)	430
BsrFI	RCCGGY	3 cut(s)	656, 1120, 1164
BsrI	ACTGG	2 cut(s)	288, 688
BssAI	RCCGGY	3 cut(s)	656, 1120, 1164
BssECI	CCNNGG	3 cut(s)	610, 730, 751
BssMI	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
BssNAI	GTATAC	1 cut(s)	211
BssT1I	CCWWGG	1 cut(s)	751
Bst1107I	GTATAC	1 cut(s)	211
Bst2UI	CCWGG	2 cut(s)	447, 858
Bst4CI	ACNGT	5 cut(s)	104, 160, 239, 365, 668
BstC8I	GCNNGC	4 cut(s)	166, 538, 1166, 1183
BstDEI	CTNAG	3 cut(s)	34, 72, 218
BstDSI	CCRYGG	1 cut(s)	730
BstF5I	GGATG	2 cut(s)	627, 1090
BstFNI	CGCG	1 cut(s)	289
BstHHI	GCGC	1 cut(s)	289
BstKTI	GATC	7 cut(s)	330, 504, 651, 834, 901, 928, 958
BstMBI	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
BstMCI	CGRYCG	2 cut(s)	651, 1111
BstMWI	GCNNNNNNNGC	2 cut(s)	650, 747
BstNI	CCWGG	2 cut(s)	447, 858
BstNSI	RCATGY	1 cut(s)	1137
BstSCI	CCNGG	4 cut(s)	202, 445, 724, 856
BstSFI	CTRYAG	1 cut(s)	159
BstUI	CGCG	1 cut(s)	289
BstV1I	GCAGC	2 cut(s)	570, 656
BstXI	CCANNNNNNTGG	1 cut(s)	176
BstZ17I	GTATAC	1 cut(s)	211
Bsu15I	ATCGAT	1 cut(s)	500
BsuRI	GGCC	4 cut(s)	403, 528, 729, 1164
BsuTUI	ATCGAT	1 cut(s)	500
BtgI	CCRYGG	1 cut(s)	730
BtgZI	GCGATG	1 cut(s)	675
BtsCI	GGATG	2 cut(s)	627, 1090
BtsIMutI	CAGTG	1 cut(s)	681
Cac8I	GCNNGC	4 cut(s)	166, 538, 1166, 1183
CfoI	GCGC	1 cut(s)	289
Cfr10I	RCCGGY	3 cut(s)	656, 1120, 1164
Cfr13I	GGNCC	6 cut(s)	251, 389, 402, 722, 727, 1118
ClaI	ATCGAT	1 cut(s)	500
CseI	GACGC	1 cut(s)	650
Csp6I	GTAC	1 cut(s)	813
CspCI	CAANNNNNGTGG	2 cut(s)	349, 384
CviAII	CATG	3 cut(s)	153, 840, 1134
CviQI	GTAC	1 cut(s)	813
DdeI	CTNAG	3 cut(s)	34, 72, 218
DpnI	GATC	7 cut(s)	329, 503, 650, 833, 900, 927, 957
DpnII	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
EaeI	YGGCCR	1 cut(s)	1162
Eco130I	CCWWGG	1 cut(s)	751
Eco24I	GRGCYC	3 cut(s)	8, 224, 847
Eco47I	GGWCC	4 cut(s)	251, 389, 722, 1118
Eco57I	CTGAAG	1 cut(s)	661
Eco88I	CYCGRG	1 cut(s)	972
EcoO109I	RGGNCCY	1 cut(s)	389
EcoRII	CCWGG	2 cut(s)	445, 856
EcoT14I	CCWWGG	1 cut(s)	751
EcoT22I	ATGCAT	1 cut(s)	154
EcoT38I	GRGCYC	3 cut(s)	8, 224, 847
ErhI	CCWWGG	1 cut(s)	751
FaeI	CATG	3 cut(s)	156, 843, 1137
FaqI	GGGAC	3 cut(s)	289, 1027, 1131
FatI	CATG	3 cut(s)	152, 839, 1133
FauI	CCCGC	3 cut(s)	282, 586, 1186
FbaI	TGATCA	1 cut(s)	925
FblI	GTMKAC	2 cut(s)	210, 966
Fnu4HI	GCNGC	3 cut(s)	559, 645, 709
FokI	GGATG	2 cut(s)	614, 1097
FriOI	GRGCYC	3 cut(s)	8, 224, 847
Fsp4HI	GCNGC	3 cut(s)	559, 645, 709
FspBI	CTAG	2 cut(s)	47, 68
GlaI	GCGC	1 cut(s)	288
GluI	GCNGC	3 cut(s)	559, 645, 709
HaeIII	GGCC	4 cut(s)	403, 528, 729, 1164
HapII	CCGG	5 cut(s)	204, 657, 725, 1121, 1165
HgaI	GACGC	1 cut(s)	650
HhaI	GCGC	1 cut(s)	289
Hin1II	CATG	3 cut(s)	156, 843, 1137
Hin6I	GCGC	1 cut(s)	287
HinP1I	GCGC	1 cut(s)	287
HincII	GTYRAC	2 cut(s)	346, 700
HindII	GTYRAC	2 cut(s)	346, 700
HindIII	AAGCTT	2 cut(s)	673, 1200
HinfI	GANTC	2 cut(s)	277, 603
HpaII	CCGG	5 cut(s)	204, 657, 725, 1121, 1165
HphI	GGTGA	4 cut(s)	125, 136, 322, 779
Hpy166II	GTNNAC	5 cut(s)	211, 346, 700, 787, 967
Hpy188I	TCNGA	6 cut(s)	260, 267, 746, 853, 1071, 1154
Hpy188III	TCNNGA	2 cut(s)	972, 1112
Hpy8I	GTNNAC	5 cut(s)	211, 346, 700, 787, 967
Hpy99I	CGWCG	1 cut(s)	602
HpyAV	CCTTC	5 cut(s)	292, 343, 402, 814, 951
HpyCH4III	ACNGT	5 cut(s)	104, 160, 239, 365, 668
HpyCH4IV	ACGT	2 cut(s)	468, 696
HpyCH4V	TGCA	7 cut(s)	152, 246, 417, 435, 479, 801, 1185
HpyF10VI	GCNNNNNNNGC	2 cut(s)	650, 747
HpyF3I	CTNAG	3 cut(s)	34, 72, 218
HpySE526I	ACGT	2 cut(s)	468, 696
Hsp92II	CATG	3 cut(s)	156, 843, 1137
HspAI	GCGC	1 cut(s)	287
KroI	GCCGGC	1 cut(s)	1164
KroNI	GCCGGC	1 cut(s)	1166
Ksp22I	TGATCA	1 cut(s)	925
Kzo9I	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
LmnI	GCTCC	2 cut(s)	11, 850
Lsp1109I	GCAGC	2 cut(s)	570, 656
LweI	GCATC	3 cut(s)	139, 570, 750
MaeI	CTAG	2 cut(s)	47, 68
MaeII	ACGT	2 cut(s)	468, 696
MaeIII	GTNAC	7 cut(s)	51, 91, 469, 618, 767, 867, 1001
MalI	GATC	7 cut(s)	329, 503, 650, 833, 900, 927, 957
MboI	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
MboII	GAAGA	2 cut(s)	998, 1084
MhlI	GDGCHC	3 cut(s)	8, 224, 847
MluCI	AATT	3 cut(s)	552, 888, 1155
MlyI	GAGTC	1 cut(s)	271
MmeI	TCCRAC	1 cut(s)	1062
MnlI	CCTC	6 cut(s)	265, 527, 620, 822, 859, 1108
Mph1103I	ATGCAT	1 cut(s)	154
MroNI	GCCGGC	1 cut(s)	1164
MroXI	GAANNNNTTC	1 cut(s)	990
MseI	TTAA	2 cut(s)	1038, 1044
MspA1I	CMGCKG	2 cut(s)	708, 1107
MspI	CCGG	5 cut(s)	204, 657, 725, 1121, 1165
MspR9I	CCNGG	4 cut(s)	204, 447, 726, 858
MvaI	CCWGG	2 cut(s)	447, 858
MvnI	CGCG	1 cut(s)	289
MwoI	GCNNNNNNNGC	2 cut(s)	650, 747
NaeI	GCCGGC	1 cut(s)	1166
NciI	CCSGG	2 cut(s)	204, 726
NdeII	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
NgoMIV	GCCGGC	1 cut(s)	1164
NlaIII	CATG	3 cut(s)	156, 843, 1137
NlaIV	GGNNCC	3 cut(s)	7, 846, 1119
NmuCI	GTSAC	6 cut(s)	51, 91, 469, 618, 767, 867
NsiI	ATGCAT	1 cut(s)	154
NspI	RCATGY	1 cut(s)	1137
PciI	ACATGT	1 cut(s)	1133
PcsI	WCGNNNNNNNCGW	4 cut(s)	540, 657, 903, 912
PdiI	GCCGGC	1 cut(s)	1166
PdmI	GAANNNNTTC	1 cut(s)	990
PfeI	GAWTC	1 cut(s)	603
PflMI	CCANNNNNTGG	1 cut(s)	637
PkrI	GCNGC	3 cut(s)	560, 646, 710
Ple19I	CGATCG	1 cut(s)	651
PleI	GAGTC	1 cut(s)	271
PpsI	GAGTC	1 cut(s)	271
PpuMI	RGGWCCY	1 cut(s)	389
PscI	ACATGT	1 cut(s)	1133
Psp5II	RGGWCCY	1 cut(s)	389
Psp6I	CCWGG	2 cut(s)	445, 856
PspGI	CCWGG	2 cut(s)	445, 856
PspN4I	GGNNCC	3 cut(s)	7, 846, 1119
PspPI	GGNCC	6 cut(s)	251, 389, 402, 722, 727, 1118
PspPPI	RGGWCCY	1 cut(s)	389
PvuI	CGATCG	1 cut(s)	651
RgaI	GCGATCGC	1 cut(s)	651
RsaI	GTAC	1 cut(s)	814
RsaNI	GTAC	1 cut(s)	813
SaqAI	TTAA	2 cut(s)	1038, 1044
SatI	GCNGC	3 cut(s)	559, 645, 709
Sau3AI	GATC	7 cut(s)	327, 501, 648, 831, 898, 925, 955
Sau96I	GGNCC	6 cut(s)	251, 389, 402, 722, 727, 1118
SchI	GAGTC	1 cut(s)	271
ScrFI	CCNGG	4 cut(s)	204, 447, 726, 858
SduI	GDGCHC	3 cut(s)	8, 224, 847
SfaAI	GCGATCGC	1 cut(s)	651
SfaNI	GCATC	3 cut(s)	139, 570, 750
SfcI	CTRYAG	1 cut(s)	159
SgfI	GCGATCGC	1 cut(s)	651
SgrAI	CRCCGGYG	1 cut(s)	656
SinI	GGWCC	4 cut(s)	251, 389, 722, 1118
Sse9I	AATT	3 cut(s)	552, 888, 1155
SsiI	CCGC	5 cut(s)	289, 579, 708, 1107, 1179
SspI	AATATT	2 cut(s)	305, 935
SspMI	CTAG	2 cut(s)	47, 68
StyD4I	CCNGG	4 cut(s)	202, 445, 724, 856
StyI	CCWWGG	1 cut(s)	751
TaaI	ACNGT	5 cut(s)	104, 160, 239, 365, 668
TaiI	ACGT	2 cut(s)	471, 699
TaqI	TCGA	6 cut(s)	87, 500, 882, 897, 906, 984
TasI	AATT	3 cut(s)	552, 888, 1155
TauI	GCSGC	1 cut(s)	711
TfiI	GAWTC	1 cut(s)	603
Tru1I	TTAA	2 cut(s)	1038, 1044
Tru9I	TTAA	2 cut(s)	1038, 1044
TscAI	CASTG	1 cut(s)	688
TseFI	GTSAC	6 cut(s)	51, 91, 469, 618, 767, 867
TseI	GCWGC	2 cut(s)	558, 644
Tsp45I	GTSAC	6 cut(s)	51, 91, 469, 618, 767, 867
TspGWI	ACGGA	2 cut(s)	615, 747
TspRI	CASTG	1 cut(s)	688
Van91I	CCANNNNNTGG	1 cut(s)	637
VpaK11BI	GGWCC	4 cut(s)	251, 389, 722, 1118
XapI	RAATTY	2 cut(s)	888, 1155
XceI	RCATGY	1 cut(s)	1137
XmiI	GTMKAC	2 cut(s)	210, 966
XmnI	GAANNNNTTC	1 cut(s)	990
XspI	CTAG	2 cut(s)	47, 68
Zsp2I	ATGCAT	1 cut(s)	154

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.