Rh5BG287500

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002146
Physical Location & Seq
Forward (+)
36619941 .. 36624020
4080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG287500.1

Sequence Viewer

Length: 897 bp
ATGTCTTTGTTCATAAAGAGCTCGAGCATGGTTATTAAATGTGTTTGGTTATTGTTGTTGGTATTACTCATCAACAATGTTCAAGCTCAGATGAAAACTTTTAATGTAAACCAATATGGTGGTGTTGCAGATGGAAAAACTGATAACAGCAAGGCTTTTACTGATGCATGGAATCAAGCTTGCCAAAACAATGGAGGTGGTGTGGGTACCTTATTGGCTTCAAGCGAGTACCAGTACTCGGTTGGTATTGACCATTGGATCGTCTTCAGATACATTGACAACTTAGTAATTAGTGGTGGAGGGACGTTCGATGGCCAAGGAGCCTCTTCTTGGCCCTATAACGACTGTCGCAAAAACCCACAGTGCAAGGCACTGCCTGCGTCATTGAGGTTCGACTTTGCCAATAATACAAAGATCGACAACATAACTCTCATCAACAGCAAGAACACCAACATCCACCTTTTCGCAGGTCTTAATGTGATAATCAGCAACATTAACATAAGTGCCCCGGCCGACAGCCCCAACACCGATGGAATCAAAGTCGGTAGTTCAAGCATCGTCCAAATATATGATTCTGTAATATCCACCGGAGATGACTGCATAGCTTTCCTTCCTGAATCAACCAACCTTAACGTTACTAACGTTCACTGCGGGCCTGGCCATGGAATTAGTGTAGGAAGCATTTCCAGTAACTCTATTACTGGCTTGAACGTAAGGAATTGCAGCTTCGTAGGTACTCAGAATGGTGTTAGAATCAAGACGAAGAGTCCCTCTCAACCAGGAACTGTTGCACAAGTTACATTTGAAAACATTGAAATGGATAAGGTCGATAATCCCATCATCATTGATCAACAATATTGCGCTAGTAGTGGTTGTAGTCCACCGGTAAGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

298

Amino Acids

31.83

Weight (kDa)

5.75

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 76 - 296 1.5e-69 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 458
Acc65I GGTACC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 206
AciI CCGC 1 cut(s) 651
AclI AACGTT 2 cut(s) 633, 642
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 3 cut(s) 313, 510, 658
AcuI CTGAAG 1 cut(s) 250
AfaI GTAC 4 cut(s) 208, 230, 236, 736
AfiI CCNNNNNNNGG 3 cut(s) 238, 330, 662
AgeI ACCGGT 1 cut(s) 883
AgsI TTSAA 6 cut(s) 83, 222, 552, 709, 806, 815
AhdI GACNNNNNGTC 1 cut(s) 765
AjnI CCWGG 2 cut(s) 655, 778
AluBI AGCT 5 cut(s) 21, 86, 179, 605, 726
AluI AGCT 5 cut(s) 21, 86, 179, 605, 726
Alw21I GWGCWC 1 cut(s) 23
AlwI GGATC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 5 cut(s) 313, 332, 510, 653, 658
ApeKI GCWGC 1 cut(s) 723
AsiGI ACCGGT 1 cut(s) 883
Asp700I GAANNNNTTC 1 cut(s) 682
Asp718I GGTACC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 863
AspS9I GGNCC 2 cut(s) 333, 653
AsuC2I CCSGG 1 cut(s) 509
AvaI CYCGRG 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 508
BalI TGGCCA 2 cut(s) 315, 660
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 735
BccI CCATC 4 cut(s) 125, 305, 524, 845
BciT130I CCWGG 2 cut(s) 657, 780
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 1 cut(s) 509
BfaI CTAG 1 cut(s) 864
BfuAI ACCTGC 1 cut(s) 458
BisI GCNGC 1 cut(s) 724
BlsI GCNGC 1 cut(s) 725
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 3 cut(s) 509, 657, 780
BmeRI GACNNNNNGTC 1 cut(s) 765
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 333, 653
BmiI GGNNCC 2 cut(s) 208, 322
BmrFI CCNGG 3 cut(s) 509, 657, 780
BmsI GCATC 2 cut(s) 154, 564
BpiI GAAGAC 1 cut(s) 256
BplI GAGNNNNNCTC 2 cut(s) 757, 789
BpuMI CCSGG 1 cut(s) 509
BsaJI CCNNGG 3 cut(s) 316, 507, 661
BsaWI WCCGGW 2 cut(s) 587, 883
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 238, 330, 662
Bse118I RCCGGY 1 cut(s) 883
Bse1I ACTGG 3 cut(s) 232, 687, 706
BseBI CCWGG 2 cut(s) 657, 780
BseDI CCNNGG 3 cut(s) 316, 507, 661
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 3 cut(s) 238, 330, 662
BseMII CTCAG 2 cut(s) 101, 752
BseNI ACTGG 3 cut(s) 232, 687, 706
BseSI GKGCMC 1 cut(s) 508
BseX3I CGGCCG 1 cut(s) 510
BseXI GCAGC 1 cut(s) 735
Bsh1285I CGRYCG 1 cut(s) 513
BshFI GGCC 5 cut(s) 315, 334, 512, 655, 660
BshNI GGYRCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 883
BsiEI CGRYCG 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 3 cut(s) 509, 588, 884
BslFI GGGAC 2 cut(s) 316, 753
BslI CCNNNNNNNGG 3 cut(s) 238, 330, 662
BsmFI GGGAC 2 cut(s) 316, 753
BsnI GGCC 5 cut(s) 315, 334, 512, 655, 660
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 23, 508
Bsp143I GATC 3 cut(s) 258, 414, 847
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 1 cut(s) 651
BspANI GGCC 5 cut(s) 315, 334, 512, 655, 660
BspCNI CTCAG 2 cut(s) 100, 751
BspLI GGNNCC 2 cut(s) 208, 322
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 883
BsrI ACTGG 3 cut(s) 232, 687, 706
BssAI RCCGGY 1 cut(s) 883
BssECI CCNNGG 3 cut(s) 316, 507, 661
BssMI GATC 3 cut(s) 258, 414, 847
BssT1I CCWWGG 2 cut(s) 316, 661
Bst2UI CCWGG 2 cut(s) 657, 780
Bst4CI ACNGT 3 cut(s) 347, 363, 787
Bst6I CTCTTC 2 cut(s) 331, 758
BstAPI GCANNNNNTGC 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 181, 378, 653
BstDEI CTNAG 3 cut(s) 87, 283, 738
BstDSI CCRYGG 1 cut(s) 661
BstF5I GGATG 1 cut(s) 453
BstHHI GCGC 1 cut(s) 863
BstKTI GATC 3 cut(s) 261, 417, 850
BstMBI GATC 3 cut(s) 258, 414, 847
BstMCI CGRYCG 1 cut(s) 513
BstMWI GCNNNNNNNGC 2 cut(s) 377, 657
BstNI CCWGG 2 cut(s) 657, 780
BstSCI CCNGG 3 cut(s) 507, 655, 778
BstSLI GKGCMC 1 cut(s) 508
BstV1I GCAGC 1 cut(s) 735
BstV2I GAAGAC 1 cut(s) 256
BstXI CCANNNNNNTGG 2 cut(s) 119, 191
BstZI CGGCCG 1 cut(s) 510
BsuRI GGCC 5 cut(s) 315, 334, 512, 655, 660
BtgI CCRYGG 1 cut(s) 661
BtsCI GGATG 1 cut(s) 453
BtsI GCAGTG 2 cut(s) 371, 646
BtsIMutI CAGTG 3 cut(s) 368, 371, 646
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 3 cut(s) 181, 378, 653
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 1 cut(s) 863
Cfr10I RCCGGY 1 cut(s) 883
Cfr13I GGNCC 2 cut(s) 333, 653
CseI GACGC 1 cut(s) 369
Csp6I GTAC 4 cut(s) 207, 229, 235, 735
CspAI ACCGGT 1 cut(s) 883
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 3 cut(s) 28, 168, 662
CviQI GTAC 4 cut(s) 207, 229, 235, 735
DdeI CTNAG 3 cut(s) 87, 283, 738
DpnI GATC 3 cut(s) 260, 416, 849
DpnII GATC 3 cut(s) 258, 414, 847
DriI GACNNNNNGTC 1 cut(s) 765
EaeI YGGCCR 3 cut(s) 313, 510, 658
EagI CGGCCG 1 cut(s) 510
Eam1104I CTCTTC 2 cut(s) 331, 758
Eam1105I GACNNNNNGTC 1 cut(s) 765
EarI CTCTTC 2 cut(s) 331, 758
Ecl136II GAGCTC 1 cut(s) 21
EclXI CGGCCG 1 cut(s) 510
Eco130I CCWWGG 2 cut(s) 316, 661
Eco24I GRGCYC 1 cut(s) 23
Eco52I CGGCCG 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 2 cut(s) 655, 778
EcoT14I CCWWGG 2 cut(s) 316, 661
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 2 cut(s) 316, 661
FaeI CATG 3 cut(s) 31, 171, 665
FaqI GGGAC 2 cut(s) 316, 753
FatI CATG 3 cut(s) 27, 167, 661
FauI CCCGC 1 cut(s) 644
FbaI TGATCA 1 cut(s) 847
Fnu4HI GCNGC 1 cut(s) 724
FokI GGATG 1 cut(s) 440
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 1 cut(s) 724
FspBI CTAG 1 cut(s) 864
GlaI GCGC 1 cut(s) 862
GluI GCNGC 1 cut(s) 724
HaeIII GGCC 5 cut(s) 315, 334, 512, 655, 660
HapII CCGG 3 cut(s) 509, 588, 884
HgaI GACGC 1 cut(s) 369
HhaI GCGC 1 cut(s) 863
Hin1II CATG 3 cut(s) 31, 171, 665
Hin6I GCGC 1 cut(s) 861
HinP1I GCGC 1 cut(s) 861
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 6 cut(s) 172, 534, 572, 617, 753, 766
HpaII CCGG 3 cut(s) 509, 588, 884
Hpy166II GTNNAC 3 cut(s) 109, 646, 881
Hpy188I TCNGA 3 cut(s) 90, 269, 741
Hpy188III TCNNGA 2 cut(s) 614, 757
Hpy8I GTNNAC 3 cut(s) 109, 646, 881
HpyAV CCTTC 1 cut(s) 620
HpyCH4III ACNGT 3 cut(s) 347, 363, 787
HpyCH4IV ACGT 4 cut(s) 305, 633, 642, 711
HpyCH4V TGCA 6 cut(s) 128, 167, 366, 600, 723, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 657
HpyF3I CTNAG 3 cut(s) 87, 283, 738
HpySE526I ACGT 4 cut(s) 305, 633, 642, 711
Hsp92II CATG 3 cut(s) 31, 171, 665
HspAI GCGC 1 cut(s) 861
KpnI GGTACC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 3 cut(s) 258, 414, 847
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 1 cut(s) 735
LweI GCATC 2 cut(s) 154, 564
MaeI CTAG 1 cut(s) 864
MaeII ACGT 4 cut(s) 305, 633, 642, 711
MaeIII GTNAC 3 cut(s) 634, 689, 796
MalI GATC 3 cut(s) 260, 416, 849
MboI GATC 3 cut(s) 258, 414, 847
MboII GAAGA 3 cut(s) 256, 318, 775
MhlI GDGCHC 2 cut(s) 23, 508
MlsI TGGCCA 2 cut(s) 315, 660
MluCI AATT 4 cut(s) 288, 666, 718, 892
MluNI TGGCCA 2 cut(s) 315, 660
MlyI GAGTC 1 cut(s) 775
MnlI CCTC 5 cut(s) 188, 293, 334, 381, 781
Mox20I TGGCCA 2 cut(s) 315, 660
Mph1103I ATGCAT 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 682
MscI TGGCCA 2 cut(s) 315, 660
MseI TTAA 6 cut(s) 36, 102, 474, 495, 630, 895
MslI CAYNNNNRTG 1 cut(s) 815
Msp20I TGGCCA 2 cut(s) 315, 660
MspI CCGG 3 cut(s) 509, 588, 884
MspR9I CCNGG 3 cut(s) 509, 657, 780
MvaI CCWGG 2 cut(s) 657, 780
MwoI GCNNNNNNNGC 2 cut(s) 377, 657
NciI CCSGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 661
NdeII GATC 3 cut(s) 258, 414, 847
NlaIII CATG 3 cut(s) 31, 171, 665
NlaIV GGNNCC 2 cut(s) 208, 322
NsiI ATGCAT 1 cut(s) 169
PaeR7I CTCGAG 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 682
PfeI GAWTC 5 cut(s) 172, 534, 572, 617, 753
PinAI ACCGGT 1 cut(s) 883
PkrI GCNGC 1 cut(s) 725
PleI GAGTC 1 cut(s) 774
PpsI GAGTC 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 23
Psp1406I AACGTT 2 cut(s) 633, 642
Psp6I CCWGG 2 cut(s) 655, 778
PspGI CCWGG 2 cut(s) 655, 778
PspN4I GGNNCC 2 cut(s) 208, 322
PspPI GGNCC 2 cut(s) 333, 653
PspXI VCTCGAGB 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 785
RsaI GTAC 4 cut(s) 208, 230, 236, 736
RsaNI GTAC 4 cut(s) 207, 229, 235, 735
RseI CAYNNNNRTG 1 cut(s) 815
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 6 cut(s) 36, 102, 474, 495, 630, 895
SatI GCNGC 1 cut(s) 724
Sau3AI GATC 3 cut(s) 258, 414, 847
Sau96I GGNCC 2 cut(s) 333, 653
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 3 cut(s) 509, 657, 780
SduI GDGCHC 2 cut(s) 23, 508
SfaNI GCATC 2 cut(s) 154, 564
Sfr274I CTCGAG 1 cut(s) 22
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 815
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
Sse9I AATT 4 cut(s) 288, 666, 718, 892
SsiI CCGC 1 cut(s) 651
SspI AATATT 1 cut(s) 857
SspMI CTAG 1 cut(s) 864
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 3 cut(s) 507, 655, 778
StyI CCWWGG 2 cut(s) 316, 661
TaaI ACNGT 3 cut(s) 347, 363, 787
TaiI ACGT 4 cut(s) 308, 636, 645, 714
TaqI TCGA 5 cut(s) 23, 309, 393, 417, 828
TasI AATT 4 cut(s) 288, 666, 718, 892
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 5 cut(s) 172, 534, 572, 617, 753
Tru1I TTAA 6 cut(s) 36, 102, 474, 495, 630, 895
Tru9I TTAA 6 cut(s) 36, 102, 474, 495, 630, 895
TscAI CASTG 3 cut(s) 368, 378, 653
TseI GCWGC 1 cut(s) 723
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 3 cut(s) 368, 378, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XhoI CTCGAG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 1 cut(s) 864
ZrmI AGTACT 1 cut(s) 236
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.