Rh5CG318800

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002146
Physical Location & Seq
Forward (+)
35401862 .. 35411088
9227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG318800.1

Sequence Viewer

Length: 1143 bp
ATGTCTTTGTTCATAAAGAGCTCGAGCATGGTTATTAAATGTGTTTGGTTATTGTTGTTGGTATTACTCATCAACAATGTTCAAGCTCAGATGAAAACTTTCTATATAAACCAATATGGTGGTATTGCAGATGGAAAAACTGATAACAGCAAGGCTTTTACTGATGCATGGAATCAAGCTTGCCAAAACAATGGAGGTGGTGTGGTCTTGTTTCAGCAAGGGATATATTTGGTGAACCCTTTGGTTATAGAAGGCTCTTGCAAGGGACCGATGGAGTTGCAGATCCAGGGTACCTTATTGGCTTCAAGCGAGTACCAGTACTCGGTTGGTATTGACCATTGGATCGTCTTCCGACACATTGACAACTTAGTAATTAGTGGTGGAGGGACGTTGGATGGCCAAGGAGCCTCTTCTTGGCCCTATAACGACTGTCGCAAAAACCCACAGTGCAAGGCACTGCCTACATCATTGAGGTTCGACTTTGCCAATAATACAAAGATCGACAACATAACTCTCATCAACAGCAAGAACACCAACATCCACCTTTTCGCAGGTTTTAATGTGATAATCAGCAACATTAACATAAGTGCCCCGGCCGACAGCCCCAACACCGATGGAATCAAAGTCGGTAGTTCAAGCATCGTCCAAATATATGATTCTGTAATATCCACCGGAGATGACTGCATAGCTTTCCTTCCTGAATCAACCAACCTTAACGTTACTAACGTTCACTGCGGGCCTGGCCATGGAATTAGTGTAGGAAGCATTTCCAGTAACTCTATTACTGGCTTGAACGTAAGGAATTGCAGCTTCGTAGGTACTCAGAATGGTGTTAGAATCAAGACGAAGAGTCCCTCTCAACCAGGAACTGTTGCACAAGTTACATTTGAAAACATTGAAATGGATAAGGTCGGTAATCCCATCATCATTGATCAACAATATTGCGCTAGTAGTGGTTGTAGTCCACCGAGAGAGGTATCCGAGTCAAATTCCTCAAGCGCTAGGGTTCCAAAGTTTGAACCTTTAATCCGAGGGGAGAAAGGAAGAAACAGAAGAAAAAGGGGGAGACGGGAAGGACAGAGGAGGCAGAGGAATCCAAAAGAGGAGAAGAACTGCTCTACTGCACAGATTCAAGAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

380

Amino Acids

41.43

Weight (kDa)

8.71

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 75 - 323 6.7e-75 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 542
Acc65I GGTACC 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 290
AciI CCGC 1 cut(s) 735
AclI AACGTT 2 cut(s) 717, 726
AclWI GGATC 2 cut(s) 277, 350
AcoI YGGCCR 3 cut(s) 397, 594, 742
AcsI RAATTY 1 cut(s) 988
AfaI GTAC 4 cut(s) 292, 314, 320, 820
AfeI AGCGCT 1 cut(s) 1000
AfiI CCNNNNNNNGG 3 cut(s) 322, 414, 746
AgsI TTSAA 8 cut(s) 83, 306, 636, 793, 890, 899, 1019, 1133
AhdI GACNNNNNGTC 1 cut(s) 849
AjnI CCWGG 3 cut(s) 285, 739, 862
AloI GAACNNNNNNTCC 2 cut(s) 1011, 1043
AluBI AGCT 5 cut(s) 21, 86, 179, 689, 810
AluI AGCT 5 cut(s) 21, 86, 179, 689, 810
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 1060
AlwI GGATC 2 cut(s) 277, 350
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 1 cut(s) 22
Aor51HI AGCGCT 1 cut(s) 1000
AoxI GGCC 5 cut(s) 397, 416, 594, 737, 742
ApeKI GCWGC 1 cut(s) 807
ApoI RAATTY 1 cut(s) 988
Asp700I GAANNNNTTC 2 cut(s) 98, 766
Asp718I GGTACC 1 cut(s) 290
AspLEI GCGC 2 cut(s) 947, 1001
AspS9I GGNCC 3 cut(s) 266, 417, 737
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 592
BalI TGGCCA 2 cut(s) 399, 744
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 819
BccI CCATC 5 cut(s) 125, 265, 389, 608, 929
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 3 cut(s) 287, 741, 864
BciVI GTATCC 1 cut(s) 988
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 1 cut(s) 1060
BfaI CTAG 2 cut(s) 948, 1002
BfoI RGCGCY 1 cut(s) 1002
BfuAI ACCTGC 1 cut(s) 542
BfuI GTATCC 1 cut(s) 988
BisI GCNGC 1 cut(s) 808
BlsI GCNGC 1 cut(s) 809
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 4 cut(s) 287, 593, 741, 864
Bme18I GGWCC 1 cut(s) 266
BmeRI GACNNNNNGTC 1 cut(s) 849
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 266, 417, 737
BmiI GGNNCC 4 cut(s) 267, 292, 406, 1008
BmrFI CCNGG 4 cut(s) 287, 593, 741, 864
BmsI GCATC 2 cut(s) 154, 648
BpiI GAAGAC 1 cut(s) 340
BplI GAGNNNNNCTC 2 cut(s) 841, 873
BpuEI CTTGAG 1 cut(s) 979
BpuMI CCSGG 1 cut(s) 593
BsaJI CCNNGG 5 cut(s) 286, 400, 591, 745, 1030
BsaWI WCCGGW 1 cut(s) 671
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 414, 746
Bse1I ACTGG 3 cut(s) 316, 771, 790
BseBI CCWGG 3 cut(s) 287, 741, 864
BseDI CCNNGG 5 cut(s) 286, 400, 591, 745, 1030
BseGI GGATG 2 cut(s) 400, 537
BseLI CCNNNNNNNGG 3 cut(s) 322, 414, 746
BseMII CTCAG 2 cut(s) 101, 836
BseNI ACTGG 3 cut(s) 316, 771, 790
BseRI GAGGAG 2 cut(s) 1096, 1118
BseSI GKGCMC 1 cut(s) 592
BseX3I CGGCCG 1 cut(s) 594
BseXI GCAGC 1 cut(s) 819
BsgI GTGCAG 1 cut(s) 1107
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 5 cut(s) 399, 418, 596, 739, 744
BshNI GGYRCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 2 cut(s) 593, 672
BslFI GGGAC 3 cut(s) 279, 400, 837
BslI CCNNNNNNNGG 3 cut(s) 322, 414, 746
BsmAI GTCTC 1 cut(s) 1060
BsmBI CGTCTC 1 cut(s) 1060
BsmFI GGGAC 3 cut(s) 279, 400, 837
BsnI GGCC 5 cut(s) 399, 418, 596, 739, 744
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 23, 592
Bsp143I GATC 4 cut(s) 282, 342, 498, 931
Bsp19I CCATGG 1 cut(s) 745
BspACI CCGC 1 cut(s) 735
BspANI GGCC 5 cut(s) 399, 418, 596, 739, 744
BspCNI CTCAG 2 cut(s) 100, 835
BspLI GGNNCC 4 cut(s) 267, 292, 406, 1008
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 2 cut(s) 277, 350
BspT107I GGYRCC 1 cut(s) 290
BsrI ACTGG 3 cut(s) 316, 771, 790
BssECI CCNNGG 5 cut(s) 286, 400, 591, 745, 1030
BssMI GATC 4 cut(s) 282, 342, 498, 931
BssT1I CCWWGG 2 cut(s) 400, 745
Bst2UI CCWGG 3 cut(s) 287, 741, 864
Bst4CI ACNGT 3 cut(s) 431, 447, 871
Bst6I CTCTTC 2 cut(s) 415, 842
BstC8I GCNNGC 2 cut(s) 181, 737
BstDEI CTNAG 3 cut(s) 87, 367, 822
BstDSI CCRYGG 1 cut(s) 745
BstF5I GGATG 2 cut(s) 400, 537
BstH2I RGCGCY 1 cut(s) 1002
BstHHI GCGC 2 cut(s) 947, 1001
BstKTI GATC 4 cut(s) 285, 345, 501, 934
BstMAI GTCTC 1 cut(s) 1060
BstMBI GATC 4 cut(s) 282, 342, 498, 931
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 1 cut(s) 741
BstNI CCWGG 3 cut(s) 287, 741, 864
BstSCI CCNGG 4 cut(s) 285, 591, 739, 862
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 819
BstV2I GAAGAC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 282
BstXI CCANNNNNNTGG 2 cut(s) 119, 191
BstYI RGATCY 1 cut(s) 282
BstZI CGGCCG 1 cut(s) 594
BsuI GTATCC 1 cut(s) 988
BsuRI GGCC 5 cut(s) 399, 418, 596, 739, 744
BtgI CCRYGG 1 cut(s) 745
BtsCI GGATG 2 cut(s) 400, 537
BtsI GCAGTG 2 cut(s) 455, 730
BtsIMutI CAGTG 3 cut(s) 452, 455, 730
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 2 cut(s) 181, 737
CaiI CAGNNNCTG 1 cut(s) 869
CfoI GCGC 2 cut(s) 947, 1001
Cfr13I GGNCC 3 cut(s) 266, 417, 737
Csp6I GTAC 4 cut(s) 291, 313, 319, 819
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 3 cut(s) 28, 168, 746
CviQI GTAC 4 cut(s) 291, 313, 319, 819
DdeI CTNAG 3 cut(s) 87, 367, 822
DpnI GATC 4 cut(s) 284, 344, 500, 933
DpnII GATC 4 cut(s) 282, 342, 498, 931
DriI GACNNNNNGTC 1 cut(s) 849
EaeI YGGCCR 3 cut(s) 397, 594, 742
EagI CGGCCG 1 cut(s) 594
Eam1104I CTCTTC 2 cut(s) 415, 842
Eam1105I GACNNNNNGTC 1 cut(s) 849
EarI CTCTTC 2 cut(s) 415, 842
Ecl136II GAGCTC 1 cut(s) 21
EclXI CGGCCG 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 400, 745
Eco24I GRGCYC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 266
Eco47III AGCGCT 1 cut(s) 1000
Eco52I CGGCCG 1 cut(s) 594
Eco53kI GAGCTC 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 3 cut(s) 285, 739, 862
EcoT14I CCWWGG 2 cut(s) 400, 745
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 2 cut(s) 400, 745
Esp3I CGTCTC 1 cut(s) 1060
FaeI CATG 3 cut(s) 31, 171, 749
FaqI GGGAC 3 cut(s) 279, 400, 837
FatI CATG 3 cut(s) 27, 167, 745
FauI CCCGC 1 cut(s) 728
FbaI TGATCA 1 cut(s) 931
Fnu4HI GCNGC 1 cut(s) 808
FokI GGATG 2 cut(s) 407, 524
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 1 cut(s) 808
FspBI CTAG 2 cut(s) 948, 1002
GlaI GCGC 2 cut(s) 946, 1000
GluI GCNGC 1 cut(s) 808
HaeII RGCGCY 1 cut(s) 1002
HaeIII GGCC 5 cut(s) 399, 418, 596, 739, 744
HapII CCGG 2 cut(s) 593, 672
HhaI GCGC 2 cut(s) 947, 1001
Hin1II CATG 3 cut(s) 31, 171, 749
Hin6I GCGC 2 cut(s) 945, 999
HinP1I GCGC 2 cut(s) 945, 999
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 9 cut(s) 172, 618, 656, 701, 837, 850, 983, 1093, 1129
HpaII CCGG 2 cut(s) 593, 672
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 3 cut(s) 235, 730, 965
Hpy188I TCNGA 5 cut(s) 90, 353, 825, 982, 1031
Hpy188III TCNNGA 3 cut(s) 698, 841, 1133
Hpy8I GTNNAC 3 cut(s) 235, 730, 965
HpyAV CCTTC 3 cut(s) 245, 704, 1067
HpyCH4III ACNGT 3 cut(s) 431, 447, 871
HpyCH4IV ACGT 4 cut(s) 389, 717, 726, 795
HpyCH4V TGCA 9 cut(s) 128, 167, 261, 280, 450, 684, 807, 875, 1124
HpyF10VI GCNNNNNNNGC 1 cut(s) 741
HpyF3I CTNAG 3 cut(s) 87, 367, 822
HpySE526I ACGT 4 cut(s) 389, 717, 726, 795
Hsp92II CATG 3 cut(s) 31, 171, 749
HspAI GCGC 2 cut(s) 945, 999
KpnI GGTACC 1 cut(s) 294
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 4 cut(s) 282, 342, 498, 931
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 1 cut(s) 819
LweI GCATC 2 cut(s) 154, 648
MaeI CTAG 2 cut(s) 948, 1002
MaeII ACGT 4 cut(s) 389, 717, 726, 795
MaeIII GTNAC 3 cut(s) 718, 773, 880
MalI GATC 4 cut(s) 284, 344, 500, 933
MboI GATC 4 cut(s) 282, 342, 498, 931
MboII GAAGA 6 cut(s) 340, 402, 859, 1056, 1065, 1120
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 2 cut(s) 23, 592
MlsI TGGCCA 2 cut(s) 399, 744
MluCI AATT 4 cut(s) 372, 750, 802, 988
MluNI TGGCCA 2 cut(s) 399, 744
MlyI GAGTC 2 cut(s) 859, 992
MmeI TCCRAC 2 cut(s) 372, 376
Mox20I TGGCCA 2 cut(s) 399, 744
Mph1103I ATGCAT 1 cut(s) 169
MroXI GAANNNNTTC 2 cut(s) 98, 766
MscI TGGCCA 2 cut(s) 399, 744
MseI TTAA 5 cut(s) 36, 558, 579, 714, 1025
MslI CAYNNNNRTG 1 cut(s) 899
Msp20I TGGCCA 2 cut(s) 399, 744
MspI CCGG 2 cut(s) 593, 672
MspR9I CCNGG 4 cut(s) 287, 593, 741, 864
MvaI CCWGG 3 cut(s) 287, 741, 864
MwoI GCNNNNNNNGC 1 cut(s) 741
NciI CCSGG 1 cut(s) 593
NcoI CCATGG 1 cut(s) 745
NdeII GATC 4 cut(s) 282, 342, 498, 931
NlaIII CATG 3 cut(s) 31, 171, 749
NlaIV GGNNCC 4 cut(s) 267, 292, 406, 1008
NsiI ATGCAT 1 cut(s) 169
PaeR7I CTCGAG 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 723
PdmI GAANNNNTTC 2 cut(s) 98, 766
PfeI GAWTC 7 cut(s) 172, 618, 656, 701, 837, 1093, 1129
PkrI GCNGC 1 cut(s) 809
PleI GAGTC 2 cut(s) 858, 991
PpsI GAGTC 2 cut(s) 858, 991
Psp124BI GAGCTC 1 cut(s) 23
Psp1406I AACGTT 2 cut(s) 717, 726
Psp6I CCWGG 3 cut(s) 285, 739, 862
PspGI CCWGG 3 cut(s) 285, 739, 862
PspN4I GGNNCC 4 cut(s) 267, 292, 406, 1008
PspPI GGNCC 3 cut(s) 266, 417, 737
PspXI VCTCGAGB 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 1 cut(s) 282
RsaI GTAC 4 cut(s) 292, 314, 320, 820
RsaNI GTAC 4 cut(s) 291, 313, 319, 819
RseI CAYNNNNRTG 1 cut(s) 899
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 5 cut(s) 36, 558, 579, 714, 1025
SatI GCNGC 1 cut(s) 808
Sau3AI GATC 4 cut(s) 282, 342, 498, 931
Sau96I GGNCC 3 cut(s) 266, 417, 737
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 2 cut(s) 859, 992
ScrFI CCNGG 4 cut(s) 287, 593, 741, 864
SduI GDGCHC 2 cut(s) 23, 592
SfaNI GCATC 2 cut(s) 154, 648
Sfr274I CTCGAG 1 cut(s) 22
SinI GGWCC 1 cut(s) 266
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 2 cut(s) 22, 994
SmoI CTYRAG 2 cut(s) 22, 994
Sse9I AATT 4 cut(s) 372, 750, 802, 988
SsiI CCGC 1 cut(s) 735
SspI AATATT 1 cut(s) 941
SspMI CTAG 2 cut(s) 948, 1002
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 4 cut(s) 285, 591, 739, 862
StyI CCWWGG 2 cut(s) 400, 745
TaaI ACNGT 3 cut(s) 431, 447, 871
TaiI ACGT 4 cut(s) 392, 720, 729, 798
TaqI TCGA 3 cut(s) 23, 477, 501
TaqII GACCGA 1 cut(s) 283
TasI AATT 4 cut(s) 372, 750, 802, 988
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 7 cut(s) 172, 618, 656, 701, 837, 1093, 1129
Tru1I TTAA 5 cut(s) 36, 558, 579, 714, 1025
Tru9I TTAA 5 cut(s) 36, 558, 579, 714, 1025
TscAI CASTG 3 cut(s) 452, 462, 737
TseI GCWGC 1 cut(s) 807
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 3 cut(s) 452, 462, 737
VpaK11BI GGWCC 1 cut(s) 266
XapI RAATTY 1 cut(s) 988
XcmI CCANNNNNNNNNTGG 1 cut(s) 323
XhoI CTCGAG 1 cut(s) 22
XmnI GAANNNNTTC 2 cut(s) 98, 766
XspI CTAG 2 cut(s) 948, 1002
ZrmI AGTACT 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.