RLG00000016994

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002146
Physical Location & Seq
Forward (+)
12001503 .. 12005430
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016994

Sequence Viewer

Length: 1128 bp
ATGGGCTTAAGCTTTTTCATCCAATGCGCTTGCTTAGTGTCGACTGTGCCGTTGCTTGGATTCCTTACCCTAGTTCATGCTCAGAGAAGGGTTTTTGATGTGACAAATTACGAACATTGGAGGAGGTATGGTGCTGTTCACAAGAGGCAAATTTGTGGTGGCTCCGGTGGTTCTTCCAGGTCCATGCAAGGGACCAATGGAGTTGGAAATTCAGGACCAATGGAGTTGGAAATTCAGGGTACCCTACAGGCTTCTAAGGAGGTCAAAGGTTTCATTGATATTGACCATTGGATCACCATACAATACGTTCACCGCTTGAAGATTAGTGGTGGTGGAACCCCAGATGGCCTCGGAGCCGATGGTTGGGGATATAATGACTGCCAAAAAACCCCAGGCCGTTGCAAGCAACTCACTGCTACACTGCGGTTGGATTTTGTCACCAAAGGTTACATAAACCACATAACATCGAACAGCAAAAACACCCACATCAATCTTTTTGCATGCAAGGACGTACTTATTCGCAACGTCAAGATATCAGCTCCCGGAGACGGTCCCAACACCGATGGAATCCACATTGGCAACTCACAGAACATCTATATCATAGACTCGCACATATCCACCGGTGAAGATTGCGTCTCCGTCCTCCCCGGATCACAATACATCAATGTCACCGGTGTCCAGTGCGGTTCAGGCCATGGAATTAGCATTGGAAGTCTCGGCAAAGGTGCTGGTGACTCTGTTAATGACATGTATGTAACACACTGCAGATTTGTGAGTACTCAAAATGCTGTGGGAATCAAAACATGGGCTGTGGATTCTAAACCTGGAATTGTTTCACGTGTTGCCTTTGAGCTCCTTGAAATGGTTAATGTTGACAATCCCATCATTATTGATCAAGAATATTGCCCTGGTGGTGGTTGTAATACACAGGAATCCTCCGAAGTTCAGATTAGTGATGTAGAGTTTAATGACATTAGAGGCACTTCCGCTACAAAGGATGCAGTTACTCTCAAATGCAGCCGAAGTAAACCTTGCCAAACTATCGAGCTGAAGGACATTGACCTAACCTTCCGAGGACCTGGAGGACCGGCGGTTTCATTGTGCTATAATGTAGAAGGCAGAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

376

Amino Acids

40.31

Weight (kDa)

6.59

Isoelectric Point (pI)

27.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 73 - 372 4.7e-78 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 41
AciI CCGC 5 cut(s) 313, 424, 684, 987, 1091
AclWI GGATC 2 cut(s) 299, 658
AcsI RAATTY 3 cut(s) 150, 208, 231
AcuI CTGAAG 1 cut(s) 1070
AcvI CACGTG 1 cut(s) 839
AfaI GTAC 3 cut(s) 241, 513, 778
AfiI CCNNNNNNNGG 6 cut(s) 56, 189, 363, 548, 862, 914
AflII CTTAAG 1 cut(s) 7
AflIII ACRYGT 2 cut(s) 747, 838
AgeI ACCGGT 2 cut(s) 620, 671
AgsI TTSAA 2 cut(s) 319, 860
AjnI CCWGG 5 cut(s) 176, 391, 823, 907, 1078
AluBI AGCT 4 cut(s) 12, 539, 853, 1048
AluI AGCT 4 cut(s) 12, 539, 853, 1048
Alw21I GWGCWC 1 cut(s) 855
Alw26I GTCTC 3 cut(s) 540, 640, 719
AlwI GGATC 2 cut(s) 299, 658
AoxI GGCC 3 cut(s) 346, 394, 691
ApeKI GCWGC 1 cut(s) 1017
ApoI RAATTY 3 cut(s) 150, 208, 231
AsiGI ACCGGT 2 cut(s) 620, 671
Asp700I GAANNNNTTC 1 cut(s) 832
Asp718I GGTACC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
AsuC2I CCSGG 2 cut(s) 543, 648
AsuHPI GGTGA 6 cut(s) 286, 302, 430, 635, 661, 743
AvaII GGWCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
BanI GGYRCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 855
BbrPI CACGTG 1 cut(s) 839
Bbv12I GWGCWC 1 cut(s) 855
BbvI GCAGC 1 cut(s) 1029
BccI CCATC 4 cut(s) 338, 353, 557, 890
BceAI ACGGC 2 cut(s) 34, 381
BciT130I CCWGG 5 cut(s) 178, 393, 825, 909, 1080
BclI TGATCA 1 cut(s) 892
BcnI CCSGG 2 cut(s) 543, 648
BcoDI GTCTC 3 cut(s) 540, 640, 719
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 2 cut(s) 245, 763
BfrI CTTAAG 1 cut(s) 7
BisI GCNGC 1 cut(s) 1018
BlsI GCNGC 1 cut(s) 1019
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 7 cut(s) 178, 393, 543, 648, 825, 909, 1080
Bme18I GGWCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
BmgT120I GGNCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
BmiI GGNNCC 6 cut(s) 163, 193, 241, 337, 355, 553
BmrFI CCNGG 7 cut(s) 178, 393, 543, 648, 825, 909, 1080
BmsI GCATC 1 cut(s) 988
BpmI CTGGAG 1 cut(s) 1101
BpuMI CCSGG 2 cut(s) 543, 648
BsaAI YACGTR 1 cut(s) 839
BsaJI CCNNGG 6 cut(s) 349, 391, 646, 694, 907, 1072
BsaWI WCCGGW 3 cut(s) 164, 620, 671
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 189, 363, 548, 862, 914
Bse118I RCCGGY 3 cut(s) 620, 671, 1087
Bse1I ACTGG 1 cut(s) 679
BseBI CCWGG 5 cut(s) 178, 393, 825, 909, 1080
BseDI CCNNGG 6 cut(s) 349, 391, 646, 694, 907, 1072
BseGI GGATG 2 cut(s) 18, 1003
BseLI CCNNNNNNNGG 6 cut(s) 56, 189, 363, 548, 862, 914
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 1 cut(s) 679
BseRI GAGGAG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 1029
BshFI GGCC 3 cut(s) 348, 396, 693
BshNI GGYRCC 1 cut(s) 239
BshTI ACCGGT 2 cut(s) 620, 671
BsiHKAI GWGCWC 1 cut(s) 855
BsiSI CCGG 6 cut(s) 165, 543, 621, 648, 672, 1088
BslFI GGGAC 2 cut(s) 205, 537
BslI CCNNNNNNNGG 6 cut(s) 56, 189, 363, 548, 862, 914
BsmAI GTCTC 3 cut(s) 540, 640, 719
BsmBI CGTCTC 2 cut(s) 540, 640
BsmFI GGGAC 2 cut(s) 205, 537
BsnI GGCC 3 cut(s) 348, 396, 693
Bsp1286I GDGCHC 1 cut(s) 855
Bsp143I GATC 3 cut(s) 291, 650, 892
Bsp19I CCATGG 1 cut(s) 694
BspACI CCGC 5 cut(s) 313, 424, 684, 987, 1091
BspANI GGCC 3 cut(s) 348, 396, 693
BspCNI CTCAG 1 cut(s) 94
BspLI GGNNCC 6 cut(s) 163, 193, 241, 337, 355, 553
BspMAI CTGCAG 1 cut(s) 767
BspPI GGATC 2 cut(s) 299, 658
BspT107I GGYRCC 1 cut(s) 239
BspTI CTTAAG 1 cut(s) 7
BsrFI RCCGGY 3 cut(s) 620, 671, 1087
BsrI ACTGG 1 cut(s) 679
BssAI RCCGGY 3 cut(s) 620, 671, 1087
BssECI CCNNGG 6 cut(s) 349, 391, 646, 694, 907, 1072
BssMI GATC 3 cut(s) 291, 650, 892
BssT1I CCWWGG 1 cut(s) 694
Bst2UI CCWGG 5 cut(s) 178, 393, 825, 909, 1080
Bst4CI ACNGT 2 cut(s) 46, 551
BstAFI CTTAAG 1 cut(s) 7
BstBAI YACGTR 1 cut(s) 839
BstC8I GCNNGC 3 cut(s) 31, 404, 502
BstDEI CTNAG 3 cut(s) 34, 81, 255
BstDSI CCRYGG 1 cut(s) 694
BstF5I GGATG 2 cut(s) 18, 1003
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 3 cut(s) 294, 653, 895
BstMAI GTCTC 3 cut(s) 540, 640, 719
BstMBI GATC 3 cut(s) 291, 650, 892
BstMWI GCNNNNNNNGC 1 cut(s) 690
BstNI CCWGG 5 cut(s) 178, 393, 825, 909, 1080
BstNSI RCATGY 2 cut(s) 504, 751
BstSCI CCNGG 7 cut(s) 176, 391, 541, 646, 823, 907, 1078
BstSFI CTRYAG 2 cut(s) 245, 763
BstV1I GCAGC 1 cut(s) 1029
BsuRI GGCC 3 cut(s) 348, 396, 693
BtgI CCRYGG 1 cut(s) 694
BtsCI GGATG 2 cut(s) 18, 1003
BtsI GCAGTG 3 cut(s) 411, 419, 760
BtsIMutI CAGTG 4 cut(s) 411, 419, 686, 760
Cac8I GCNNGC 3 cut(s) 31, 404, 502
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 3 cut(s) 620, 671, 1087
Cfr13I GGNCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
CseI GACGC 1 cut(s) 622
Csp6I GTAC 3 cut(s) 240, 512, 777
CspAI ACCGGT 2 cut(s) 620, 671
CviAII CATG 6 cut(s) 77, 184, 501, 695, 748, 804
CviQI GTAC 3 cut(s) 240, 512, 777
DdeI CTNAG 3 cut(s) 34, 81, 255
DpnI GATC 3 cut(s) 293, 652, 894
DpnII GATC 3 cut(s) 291, 650, 892
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 1 cut(s) 694
Eco24I GRGCYC 1 cut(s) 855
Eco32I GATATC 1 cut(s) 534
Eco47I GGWCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 1070
Eco72I CACGTG 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 853
EcoO109I RGGNCCY 1 cut(s) 1076
EcoRII CCWGG 5 cut(s) 176, 391, 823, 907, 1078
EcoRV GATATC 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 1 cut(s) 694
Esp3I CGTCTC 2 cut(s) 540, 640
FaeI CATG 6 cut(s) 80, 187, 504, 698, 751, 807
FalI AAGNNNNNCTT 2 cut(s) 1015, 1047
FaqI GGGAC 2 cut(s) 205, 537
FatI CATG 6 cut(s) 76, 183, 500, 694, 747, 803
FbaI TGATCA 1 cut(s) 892
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 1 cut(s) 1018
FokI GGATG 2 cut(s) 5, 1010
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 1 cut(s) 1018
FspBI CTAG 1 cut(s) 71
GlaI GCGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 1018
GsuI CTGGAG 1 cut(s) 1101
HaeIII GGCC 3 cut(s) 348, 396, 693
HapII CCGG 6 cut(s) 165, 543, 621, 648, 672, 1088
HgaI GACGC 1 cut(s) 622
HhaI GCGC 1 cut(s) 29
Hin1II CATG 6 cut(s) 80, 187, 504, 698, 751, 807
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HincII GTYRAC 2 cut(s) 42, 874
HindII GTYRAC 2 cut(s) 42, 874
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 7 cut(s) 60, 567, 605, 734, 795, 815, 932
HpaII CCGG 6 cut(s) 165, 543, 621, 648, 672, 1088
HphI GGTGA 6 cut(s) 286, 302, 430, 635, 661, 743
Hpy166II GTNNAC 5 cut(s) 42, 139, 310, 874, 1028
Hpy188I TCNGA 5 cut(s) 84, 353, 940, 948, 1073
Hpy188III TCNNGA 3 cut(s) 213, 529, 896
Hpy8I GTNNAC 5 cut(s) 42, 139, 310, 874, 1028
HpyAV CCTTC 4 cut(s) 81, 1045, 1078, 1109
HpyCH4III ACNGT 2 cut(s) 46, 551
HpyCH4IV ACGT 4 cut(s) 306, 510, 525, 838
HpyCH4V TGCA 7 cut(s) 187, 402, 500, 504, 765, 1001, 1017
HpyF10VI GCNNNNNNNGC 1 cut(s) 690
HpyF3I CTNAG 3 cut(s) 34, 81, 255
HpySE526I ACGT 4 cut(s) 306, 510, 525, 838
Hsp92II CATG 6 cut(s) 80, 187, 504, 698, 751, 807
HspAI GCGC 1 cut(s) 27
KpnI GGTACC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 892
Kzo9I GATC 3 cut(s) 291, 650, 892
LmnI GCTCC 4 cut(s) 167, 353, 544, 858
Lsp1109I GCAGC 1 cut(s) 1029
LweI GCATC 1 cut(s) 988
MaeI CTAG 1 cut(s) 71
MaeII ACGT 4 cut(s) 306, 510, 525, 838
MaeIII GTNAC 7 cut(s) 100, 436, 446, 667, 731, 754, 1003
MalI GATC 3 cut(s) 293, 652, 894
MboI GATC 3 cut(s) 291, 650, 892
MboII GAAGA 3 cut(s) 165, 331, 638
MhlI GDGCHC 1 cut(s) 855
MluCI AATT 6 cut(s) 106, 150, 208, 231, 699, 828
MlyI GAGTC 2 cut(s) 599, 728
MmeI TCCRAC 3 cut(s) 184, 207, 408
MroXI GAANNNNTTC 1 cut(s) 832
MseI TTAA 4 cut(s) 8, 741, 867, 966
MspCI CTTAAG 1 cut(s) 7
MspI CCGG 6 cut(s) 165, 543, 621, 648, 672, 1088
MspR9I CCNGG 7 cut(s) 178, 393, 543, 648, 825, 909, 1080
MvaI CCWGG 5 cut(s) 178, 393, 825, 909, 1080
MwoI GCNNNNNNNGC 1 cut(s) 690
NciI CCSGG 2 cut(s) 543, 648
NcoI CCATGG 1 cut(s) 694
NdeII GATC 3 cut(s) 291, 650, 892
NlaIII CATG 6 cut(s) 80, 187, 504, 698, 751, 807
NlaIV GGNNCC 6 cut(s) 163, 193, 241, 337, 355, 553
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 4 cut(s) 100, 436, 667, 731
NspI RCATGY 2 cut(s) 504, 751
PaeI GCATGC 1 cut(s) 504
PciI ACATGT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 832
PfeI GAWTC 5 cut(s) 60, 567, 795, 815, 932
PfoI TCCNGGA 1 cut(s) 541
PinAI ACCGGT 2 cut(s) 620, 671
PkrI GCNGC 1 cut(s) 1019
PleI GAGTC 2 cut(s) 599, 728
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
PpsI GAGTC 2 cut(s) 599, 728
Ppu21I YACGTR 1 cut(s) 839
PpuMI RGGWCCY 1 cut(s) 1076
PscI ACATGT 1 cut(s) 747
Psp124BI GAGCTC 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 1076
Psp6I CCWGG 5 cut(s) 176, 391, 823, 907, 1078
PspCI CACGTG 1 cut(s) 839
PspGI CCWGG 5 cut(s) 176, 391, 823, 907, 1078
PspN4I GGNNCC 6 cut(s) 163, 193, 241, 337, 355, 553
PspPI GGNCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
PspPPI RGGWCCY 1 cut(s) 1076
PstI CTGCAG 1 cut(s) 767
RsaI GTAC 3 cut(s) 241, 513, 778
RsaNI GTAC 3 cut(s) 240, 512, 777
SacI GAGCTC 1 cut(s) 855
SalI GTCGAC 1 cut(s) 40
SaqAI TTAA 4 cut(s) 8, 741, 867, 966
SatI GCNGC 1 cut(s) 1018
Sau3AI GATC 3 cut(s) 291, 650, 892
Sau96I GGNCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
ScaI AGTACT 1 cut(s) 778
SchI GAGTC 2 cut(s) 599, 728
ScrFI CCNGG 7 cut(s) 178, 393, 543, 648, 825, 909, 1080
SduI GDGCHC 1 cut(s) 855
SfaNI GCATC 1 cut(s) 988
SfcI CTRYAG 2 cut(s) 245, 763
SgrAI CRCCGGYG 2 cut(s) 620, 671
SinI GGWCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
SphI GCATGC 1 cut(s) 504
Sse9I AATT 6 cut(s) 106, 150, 208, 231, 699, 828
SsiI CCGC 5 cut(s) 313, 424, 684, 987, 1091
SspI AATATT 1 cut(s) 902
SspMI CTAG 1 cut(s) 71
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 7 cut(s) 176, 391, 541, 646, 823, 907, 1078
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 2 cut(s) 46, 551
TaiI ACGT 4 cut(s) 309, 513, 528, 841
TaqI TCGA 3 cut(s) 41, 467, 1044
TasI AATT 6 cut(s) 106, 150, 208, 231, 699, 828
TatI WGTACW 1 cut(s) 776
TfiI GAWTC 5 cut(s) 60, 567, 795, 815, 932
Tru1I TTAA 4 cut(s) 8, 741, 867, 966
Tru9I TTAA 4 cut(s) 8, 741, 867, 966
TscAI CASTG 4 cut(s) 418, 426, 686, 767
TseFI GTSAC 4 cut(s) 100, 436, 667, 731
TseI GCWGC 1 cut(s) 1017
Tsp45I GTSAC 4 cut(s) 100, 436, 667, 731
TspDTI ATGAA 4 cut(s) 7, 65, 262, 1086
TspGWI ACGGA 1 cut(s) 628
TspRI CASTG 4 cut(s) 418, 426, 686, 767
Vha464I CTTAAG 1 cut(s) 7
VpaK11BI GGWCC 6 cut(s) 180, 192, 215, 551, 1076, 1085
XapI RAATTY 3 cut(s) 150, 208, 231
XceI RCATGY 2 cut(s) 504, 751
XmiI GTMKAC 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 832
XspI CTAG 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.