Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0002146

Physical Location & Seq

Genomic Coordinates Reverse (-)

30152268 .. 30155660

Gene Structure 3393 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1207000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGGCTCCAGCTTGGTTGCCCAATTTGTTTTTTTAGTGGTTTTACTAGGATCCATTACCAAAGTTCATGCTGATGATGGCGCTACATCAATGAGTCACGTACACTTCTTCAGAGTTCAACTTAACCGTCAGAAAATGATTTTTAACGTGAAGAAATACGGAGGCGTTGCTGATGGCAAAACGGATAACAGCAAGGCTTTCATTGATGCATGGACTCAAGCCTGTCAAAACAATGGAGGTGGTGTGGTTTTATTCCCAGCAGGGAAATACTTGGTGAAGCCAGTGGTTTTCACAGGTGGATGCAAGGGGCCAATGGTGGTGCAGATAAAGGGAACCCTACTGGCTCCCGTGGAGGTGAAATCTTCGATTGATATTAACCATTGGATTACCTTCCAATACGTTGACAACTTGGTAATCAATGGTGGTGGTTCGTTGGATGGACAAGGTCCCTCTGCTTGGCCTTATAACAACTGCATGAACGATCCACATTGCAAGCGACTTCCTACCACCTTGAGATTGGATTTCGTCACAAATTCAAAGATCCATGACATAACATCGATCAATAGCAAGAATGTCCACATCAACCTTTTTGCGAGCCATGACATAACGATTCAAAACATCACCATATCAGCTCCCGGTGACAGCCCTAACACCGACGGAATCCACATTGGCACATCACACCACATCGAAATCTTGGATTCAGTGATCGCCACCGGGGACGATTGCATATCCTTCAGTTCCGGATCAAACAACATAAAAATCAGCGGCGTTCACTGCGGTCCGGGCCACGGAATTAGCATCGGAAGCCTTGGCAAGGGTGCTGGTGACGATGTGTCATTCGTGGACATAAGAAACTGCACCTTCGTTGGTACTACAAATGGAGTGAGAGTCAAGACATGGGCTCCCTCCCAACCAGGCACTGTTTCACATCTTACCGTTGAGTACGTTCGAGTCGATAAGGTCGATAACCCCATCATCATCGATCAACAATATTGTCCTTCACGTAGTTGCAGTACAACGGAATCCTCGAGGGTACAGATTCAAGATGTTAAGTTCAGTAATATATGGGGTAGTTCAACCACACAGAGTGCTATTACATTGAAATGTAGCCAAACTAACCCCTGCCAGAAGATTGAGATGAGGGACATCAACCTACGTTACAACGGTCCTGAAGGATCAGCTCACTCGGCATGCACAAACGCAGATGTTGTAACTTATGGCCAACAACAACCACTTCCTTGCACAGAAAAGATAGAGCTATTATACCCCTCAATATTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

428

Amino Acids

46.06

Weight (kDa)

6.72

Isoelectric Point (pI)

36.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	47 - 256	6.5e-10	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	75 - 402	1.8e-93	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002146)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g12491 FvH4_1g12500 FvH4_3g23740 FvH4_4g28770
malus_domestica	MD03G1196500.v1.1 MD11G1207000.v1.1
prunus_persica	Prupe.4G218900_v2.0.a1 Prupe.4G228600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0100401 RchiOBHm_Chr4g0436881 RchiOBHm_Chr5g0041901 RchiOBHm_Chr5g0041941
rosa_laevigata	RLG00000006489 RLG00000016970 RLG00000016994 RLG00000034104
rosa_multiflora	Rmu_sc0001095.1_g000011 Rmu_sc0001464.1_g000018 Rmu_sc0009443.1_g000011 Rmu_sc0010997.1_g000006
rosa_roxburghii	Rroxscaffold_2G00142280 Rroxscaffold_5G00377950
rosa_rugosa	Rorug02G0087800 Rorug04G0297000 Rorug05G0197100
rosa_samantha	Rh2AG137300 Rh2AG137500 Rh2BG140500 Rh2BG140700 Rh2CG142600 Rh2DG142500 Rh4AG351300 Rh4BG360300 Rh4CG374600 Rh4DG354600 Rh5AG281700 Rh5AG281900 Rh5BG287500 Rh5CG318800 Rh5DG296100 Rh5DG296400
rosa_wichuraiana	Rw0G001350 Rw2G010740 Rw4G030760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	465
AbsI	CCTCGAGG	1 cut(s)	1027
AccIII	TCCGGA	1 cut(s)	740
AciI	CCGC	2 cut(s)	765, 777
AclWI	GGATC	6 cut(s)	45, 58, 476, 535, 751, 1183
AcoI	YGGCCR	1 cut(s)	1219
AcsI	RAATTY	1 cut(s)	532
AcuI	CTGAAG	3 cut(s)	94, 718, 1191
AdeI	CACNNNGTG	1 cut(s)	1088
AfaI	GTAC	5 cut(s)	102, 871, 944, 1015, 1035
AfiI	CCNNNNNNNGG	3 cut(s)	456, 788, 814
AgsI	TTSAA	6 cut(s)	119, 537, 614, 1043, 1077, 1102
AhdI	GACNNNNNGTC	1 cut(s)	832
AjnI	CCWGG	1 cut(s)	913
AluBI	AGCT	4 cut(s)	12, 632, 1181, 1258
AluI	AGCT	4 cut(s)	12, 632, 1181, 1258
AlwI	GGATC	6 cut(s)	45, 58, 476, 535, 751, 1183
AlwNI	CAGNNNCTG	1 cut(s)	920
Ama87I	CYCGRG	1 cut(s)	1027
Aor13HI	TCCGGA	1 cut(s)	740
AoxI	GGCC	4 cut(s)	308, 458, 784, 1219
ApoI	RAATTY	1 cut(s)	532
AspLEI	GCGC	1 cut(s)	83
AspS9I	GGNCC	5 cut(s)	308, 446, 779, 784, 1166
AsuC2I	CCSGG	3 cut(s)	636, 715, 783
AsuHPI	GGTGA	5 cut(s)	286, 367, 613, 650, 836
AvaI	CYCGRG	1 cut(s)	1027
AvaII	GGWCC	3 cut(s)	446, 779, 1166
BalI	TGGCCA	1 cut(s)	1221
BamHI	GGATCC	1 cut(s)	50
BanII	GRGCYC	2 cut(s)	8, 904
BccI	CCATC	4 cut(s)	71, 167, 431, 980
BciT130I	CCWGG	1 cut(s)	915
BcnI	CCSGG	3 cut(s)	636, 715, 783
BfaI	CTAG	1 cut(s)	47
BfoI	RGCGCY	1 cut(s)	84
BisI	GCNGC	1 cut(s)	766
BlsI	GCNGC	1 cut(s)	767
Bme1390I	CCNGG	4 cut(s)	636, 715, 783, 915
Bme18I	GGWCC	3 cut(s)	446, 779, 1166
BmeRI	GACNNNNNGTC	1 cut(s)	832
BmeT110I	CYCGRG	1 cut(s)	1027
BmgT120I	GGNCC	5 cut(s)	308, 446, 779, 784, 1166
BmiI	GGNNCC	7 cut(s)	7, 52, 309, 334, 345, 448, 903
BmrFI	CCNGG	4 cut(s)	636, 715, 783, 915
BmsI	GCATC	3 cut(s)	196, 290, 807
BpuEI	CTTGAG	2 cut(s)	201, 532
BpuMI	CCSGG	3 cut(s)	636, 715, 783
Bsa29I	ATCGAT	2 cut(s)	557, 981
BsaAI	YACGTR	2 cut(s)	100, 1004
BsaJI	CCNNGG	4 cut(s)	348, 714, 787, 808
BsaWI	WCCGGW	1 cut(s)	740
BsaXI	ACNNNNNCTCC	6 cut(s)	228, 258, 873, 886, 903, 916
Bsc4I	CCNNNNNNNGG	3 cut(s)	456, 788, 814
Bse1I	ACTGG	2 cut(s)	281, 345
Bse3DI	GCAATG	1 cut(s)	487
BseAI	TCCGGA	1 cut(s)	740
BseBI	CCWGG	1 cut(s)	915
BseCI	ATCGAT	2 cut(s)	557, 981
BseDI	CCNNGG	4 cut(s)	348, 714, 787, 808
BseGI	GGATG	2 cut(s)	305, 442
BseLI	CCNNNNNNNGG	3 cut(s)	456, 788, 814
BseMI	GCAATG	1 cut(s)	487
BseNI	ACTGG	2 cut(s)	281, 345
BseYI	CCCAGC	1 cut(s)	256
BsgI	GTGCAG	2 cut(s)	341, 841
BshFI	GGCC	4 cut(s)	310, 460, 786, 1221
BshVI	ATCGAT	2 cut(s)	557, 981
BsiHKCI	CYCGRG	1 cut(s)	1027
BsiSI	CCGG	4 cut(s)	636, 714, 741, 782
BslFI	GGGAC	3 cut(s)	432, 731, 1157
BslI	CCNNNNNNNGG	3 cut(s)	456, 788, 814
BsmFI	GGGAC	3 cut(s)	432, 731, 1157
BsnI	GGCC	4 cut(s)	310, 460, 786, 1221
BsoBI	CYCGRG	1 cut(s)	1027
Bsp1286I	GDGCHC	2 cut(s)	8, 904
Bsp13I	TCCGGA	1 cut(s)	740
Bsp143I	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
BspACI	CCGC	2 cut(s)	765, 777
BspANI	GGCC	4 cut(s)	310, 460, 786, 1221
BspDI	ATCGAT	2 cut(s)	557, 981
BspEI	TCCGGA	1 cut(s)	740
BspLI	GGNNCC	7 cut(s)	7, 52, 309, 334, 345, 448, 903
BspPI	GGATC	6 cut(s)	45, 58, 476, 535, 751, 1183
BsrDI	GCAATG	1 cut(s)	487
BsrI	ACTGG	2 cut(s)	281, 345
BssECI	CCNNGG	4 cut(s)	348, 714, 787, 808
BssMI	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
BssT1I	CCWWGG	1 cut(s)	808
Bst2UI	CCWGG	1 cut(s)	915
Bst4CI	ACNGT	4 cut(s)	128, 922, 937, 1166
BstBAI	YACGTR	2 cut(s)	100, 1004
BstC8I	GCNNGC	3 cut(s)	494, 595, 1192
BstDSI	CCRYGG	2 cut(s)	348, 787
BstENI	CCTNNNNNAGG	1 cut(s)	812
BstF5I	GGATG	2 cut(s)	305, 442
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	1 cut(s)	83
BstKTI	GATC	8 cut(s)	53, 484, 543, 561, 708, 746, 985, 1178
BstMBI	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
BstMWI	GCNNNNNNNGC	4 cut(s)	774, 783, 804, 1187
BstNI	CCWGG	1 cut(s)	915
BstNSI	RCATGY	1 cut(s)	1194
BstSCI	CCNGG	4 cut(s)	634, 713, 781, 913
BstX2I	RGATCY	2 cut(s)	50, 540
BstYI	RGATCY	2 cut(s)	50, 540
Bsu15I	ATCGAT	2 cut(s)	557, 981
BsuRI	GGCC	4 cut(s)	310, 460, 786, 1221
BsuTUI	ATCGAT	2 cut(s)	557, 981
BtgI	CCRYGG	2 cut(s)	348, 787
BtsCI	GGATG	2 cut(s)	305, 442
BtsI	GCAGTG	1 cut(s)	772
BtsIMutI	CAGTG	4 cut(s)	288, 708, 772, 918
Cac8I	GCNNGC	3 cut(s)	494, 595, 1192
CaiI	CAGNNNCTG	1 cut(s)	920
CfoI	GCGC	1 cut(s)	83
Cfr13I	GGNCC	5 cut(s)	308, 446, 779, 784, 1166
ClaI	ATCGAT	2 cut(s)	557, 981
CpoI	CGGWCCG	1 cut(s)	779
Csp6I	GTAC	5 cut(s)	101, 870, 943, 1014, 1034
CspCI	CAANNNNNGTGG	4 cut(s)	220, 255, 406, 441
CspI	CGGWCCG	1 cut(s)	779
CviAII	CATG	7 cut(s)	68, 210, 475, 545, 599, 897, 1191
CviQI	GTAC	5 cut(s)	101, 870, 943, 1014, 1034
DpnI	GATC	8 cut(s)	52, 483, 542, 560, 707, 745, 984, 1177
DpnII	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
DraIII	CACNNNGTG	1 cut(s)	1088
DriI	GACNNNNNGTC	1 cut(s)	832
EaeI	YGGCCR	1 cut(s)	1219
Eam1105I	GACNNNNNGTC	1 cut(s)	832
Eco130I	CCWWGG	1 cut(s)	808
Eco24I	GRGCYC	2 cut(s)	8, 904
Eco47I	GGWCC	3 cut(s)	446, 779, 1166
Eco57I	CTGAAG	3 cut(s)	94, 718, 1191
Eco88I	CYCGRG	1 cut(s)	1027
EcoNI	CCTNNNNNAGG	1 cut(s)	812
EcoO109I	RGGNCCY	1 cut(s)	446
EcoRII	CCWGG	1 cut(s)	913
EcoT14I	CCWWGG	1 cut(s)	808
EcoT22I	ATGCAT	1 cut(s)	211
EcoT38I	GRGCYC	2 cut(s)	8, 904
ErhI	CCWWGG	1 cut(s)	808
FaeI	CATG	7 cut(s)	71, 213, 478, 548, 602, 900, 1194
FaqI	GGGAC	3 cut(s)	432, 731, 1157
FatI	CATG	7 cut(s)	67, 209, 474, 544, 598, 896, 1190
Fnu4HI	GCNGC	1 cut(s)	766
FokI	GGATG	2 cut(s)	312, 449
FriOI	GRGCYC	2 cut(s)	8, 904
Fsp4HI	GCNGC	1 cut(s)	766
FspBI	CTAG	1 cut(s)	47
GlaI	GCGC	1 cut(s)	82
GluI	GCNGC	1 cut(s)	766
GsaI	CCCAGC	1 cut(s)	260
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	4 cut(s)	310, 460, 786, 1221
HapII	CCGG	4 cut(s)	636, 714, 741, 782
HhaI	GCGC	1 cut(s)	83
Hin1II	CATG	7 cut(s)	71, 213, 478, 548, 602, 900, 1194
Hin6I	GCGC	1 cut(s)	81
HinP1I	GCGC	1 cut(s)	81
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HinfI	GANTC	9 cut(s)	94, 214, 610, 660, 698, 888, 951, 1022, 1039
HpaII	CCGG	4 cut(s)	636, 714, 741, 782
HphI	GGTGA	5 cut(s)	286, 367, 613, 650, 836
Hpy166II	GTNNAC	5 cut(s)	103, 403, 577, 772, 844
Hpy188I	TCNGA	3 cut(s)	113, 132, 803
Hpy188III	TCNNGA	4 cut(s)	741, 892, 1043, 1169
Hpy8I	GTNNAC	5 cut(s)	103, 403, 577, 772, 844
Hpy99I	CGWCG	1 cut(s)	659
HpyAV	CCTTC	5 cut(s)	400, 742, 871, 1008, 1166
HpyCH4III	ACNGT	4 cut(s)	128, 922, 937, 1166
HpyCH4IV	ACGT	6 cut(s)	99, 147, 399, 945, 1003, 1156
HpyF10VI	GCNNNNNNNGC	4 cut(s)	774, 783, 804, 1187
HpySE526I	ACGT	6 cut(s)	99, 147, 399, 945, 1003, 1156
Hsp92II	CATG	7 cut(s)	71, 213, 478, 548, 602, 900, 1194
HspAI	GCGC	1 cut(s)	81
Kpn2I	TCCGGA	1 cut(s)	740
Kzo9I	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
LmnI	GCTCC	4 cut(s)	11, 349, 637, 907
LweI	GCATC	3 cut(s)	196, 290, 807
MaeI	CTAG	1 cut(s)	47
MaeII	ACGT	6 cut(s)	99, 147, 399, 945, 1003, 1156
MaeIII	GTNAC	6 cut(s)	95, 526, 638, 824, 1157, 1210
MalI	GATC	8 cut(s)	52, 483, 542, 560, 707, 745, 984, 1177
MboI	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
MboII	GAAGA	4 cut(s)	100, 163, 354, 1141
MflI	RGATCY	2 cut(s)	50, 540
MhlI	GDGCHC	2 cut(s)	8, 904
MlsI	TGGCCA	1 cut(s)	1221
MluCI	AATT	3 cut(s)	23, 532, 792
MluNI	TGGCCA	1 cut(s)	1221
MlyI	GAGTC	4 cut(s)	103, 208, 897, 960
MmeI	TCCRAC	1 cut(s)	414
MnlI	CCTC	9 cut(s)	155, 230, 346, 460, 916, 1023, 1036, 1134, 1279
Mox20I	TGGCCA	1 cut(s)	1221
Mph1103I	ATGCAT	1 cut(s)	211
MroI	TCCGGA	1 cut(s)	740
MscI	TGGCCA	1 cut(s)	1221
MseI	TTAA	5 cut(s)	123, 144, 375, 1050, 1282
MslI	CAYNNNNRTG	2 cut(s)	72, 1102
Msp20I	TGGCCA	1 cut(s)	1221
MspA1I	CMGCKG	1 cut(s)	765
MspI	CCGG	4 cut(s)	636, 714, 741, 782
MspR9I	CCNGG	4 cut(s)	636, 715, 783, 915
MvaI	CCWGG	1 cut(s)	915
MwoI	GCNNNNNNNGC	4 cut(s)	774, 783, 804, 1187
NciI	CCSGG	3 cut(s)	636, 715, 783
NdeII	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
NlaIII	CATG	7 cut(s)	71, 213, 478, 548, 602, 900, 1194
NlaIV	GGNNCC	7 cut(s)	7, 52, 309, 334, 345, 448, 903
NmeAIII	GCCGAG	1 cut(s)	1166
NmuCI	GTSAC	4 cut(s)	95, 526, 638, 824
NsiI	ATGCAT	1 cut(s)	211
NspI	RCATGY	1 cut(s)	1194
PaeI	GCATGC	1 cut(s)	1194
PaeR7I	CTCGAG	1 cut(s)	1027
PcsI	WCGNNNNNNNCGW	3 cut(s)	951, 960, 1025
PfeI	GAWTC	5 cut(s)	610, 660, 698, 1022, 1039
PflFI	GACNNNGTC	1 cut(s)	444
PkrI	GCNGC	1 cut(s)	767
PleI	GAGTC	4 cut(s)	102, 208, 896, 959
PpsI	GAGTC	4 cut(s)	102, 208, 896, 959
Ppu21I	YACGTR	2 cut(s)	100, 1004
PpuMI	RGGWCCY	1 cut(s)	446
PsiI	TTATAA	1 cut(s)	465
Psp5II	RGGWCCY	1 cut(s)	446
Psp6I	CCWGG	1 cut(s)	913
PspFI	CCCAGC	1 cut(s)	256
PspGI	CCWGG	1 cut(s)	913
PspN4I	GGNNCC	7 cut(s)	7, 52, 309, 334, 345, 448, 903
PspPI	GGNCC	5 cut(s)	308, 446, 779, 784, 1166
PspPPI	RGGWCCY	1 cut(s)	446
PspXI	VCTCGAGB	1 cut(s)	1027
PstNI	CAGNNNCTG	1 cut(s)	920
PsuI	RGATCY	2 cut(s)	50, 540
PsyI	GACNNNGTC	1 cut(s)	444
RsaI	GTAC	5 cut(s)	102, 871, 944, 1015, 1035
RsaNI	GTAC	5 cut(s)	101, 870, 943, 1014, 1034
RseI	CAYNNNNRTG	2 cut(s)	72, 1102
Rsr2I	CGGWCCG	1 cut(s)	779
RsrII	CGGWCCG	1 cut(s)	779
SaqAI	TTAA	5 cut(s)	123, 144, 375, 1050, 1282
SatI	GCNGC	1 cut(s)	766
Sau3AI	GATC	8 cut(s)	50, 481, 540, 558, 705, 743, 982, 1175
Sau96I	GGNCC	5 cut(s)	308, 446, 779, 784, 1166
SchI	GAGTC	4 cut(s)	103, 208, 897, 960
ScrFI	CCNGG	4 cut(s)	636, 715, 783, 915
SduI	GDGCHC	2 cut(s)	8, 904
SfaNI	GCATC	3 cut(s)	196, 290, 807
Sfr274I	CTCGAG	1 cut(s)	1027
SinI	GGWCC	3 cut(s)	446, 779, 1166
SlaI	CTCGAG	1 cut(s)	1027
SmiMI	CAYNNNNRTG	2 cut(s)	72, 1102
SmlI	CTYRAG	3 cut(s)	216, 511, 1027
SmoI	CTYRAG	3 cut(s)	216, 511, 1027
SphI	GCATGC	1 cut(s)	1194
Sse9I	AATT	3 cut(s)	23, 532, 792
SsiI	CCGC	2 cut(s)	765, 777
SspI	AATATT	2 cut(s)	992, 1275
SspMI	CTAG	1 cut(s)	47
StyD4I	CCNGG	4 cut(s)	634, 713, 781, 913
StyI	CCWWGG	1 cut(s)	808
TaaI	ACNGT	4 cut(s)	128, 922, 937, 1166
TaiI	ACGT	6 cut(s)	102, 150, 402, 948, 1006, 1159
TaqI	TCGA	8 cut(s)	365, 557, 687, 949, 954, 963, 981, 1028
TasI	AATT	3 cut(s)	23, 532, 792
TatI	WGTACW	1 cut(s)	1013
TauI	GCSGC	1 cut(s)	768
TfiI	GAWTC	5 cut(s)	610, 660, 698, 1022, 1039
Tru1I	TTAA	5 cut(s)	123, 144, 375, 1050, 1282
Tru9I	TTAA	5 cut(s)	123, 144, 375, 1050, 1282
TscAI	CASTG	4 cut(s)	288, 708, 779, 925
TseFI	GTSAC	4 cut(s)	95, 526, 638, 824
Tsp45I	GTSAC	4 cut(s)	95, 526, 638, 824
TspDTI	ATGAA	3 cut(s)	56, 190, 491
TspGWI	ACGGA	5 cut(s)	174, 197, 672, 804, 1034
TspRI	CASTG	4 cut(s)	288, 708, 779, 925
Tth111I	GACNNNGTC	1 cut(s)	444
VpaK11BI	GGWCC	3 cut(s)	446, 779, 1166
XagI	CCTNNNNNAGG	1 cut(s)	812
XapI	RAATTY	1 cut(s)	532
XceI	RCATGY	1 cut(s)	1194
XcmI	CCANNNNNNNNNTGG	1 cut(s)	514
XhoI	CTCGAG	1 cut(s)	1027
XspI	CTAG	1 cut(s)	47
Zsp2I	ATGCAT	1 cut(s)	211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.