Rh5AG281900

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002146
Physical Location & Seq
Forward (+)
38217603 .. 38232082
14480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG281900.1

Sequence Viewer

Length: 726 bp
ATGAGGGAGCTGATCGGGTCGATTCGGGTCGTGCCCAGCAAAGAGTTTCAGTGCAGTGTGTGGATTGAGGAGCCAACGCTTCTGGTGACACGGTTTGAGTATGCAAATGTTTTCCACACATTCACGGATTGGTATAGCGGTTATGTAGCTTCCAGAGTCACTGGCTTACCTAGCCGGCCTCATGTTGTCTTTGTAGATGGCCATTGTAGGTCATTGAGGTTCGACTTTGCCAATAATACAAAGATCGACAACATAACTCTCATCAACAGCAAGAACACCAACATCCACCTTTTCGCAGGTCTTAATGTGATAATCAGCAACATTAACATAAGTGCCCCGGCCGACAGCCCCAACACCGATGGAATCAAAGTCGGTAGTTCAAGCATCGTCCAAATATATGATTCTGTAATATCCACCGGAGATGACTGCATAGCTTTCCTTCCTGAATCAACCAACCTTAACGTTACTAACGTTCACTGCGGGCCTGGCCGTGGAATTAGTGTAGGAAGCATTTCCAGTAACTCTATTACTGGCTTGAACGTAAGGAATTGCAGCTTCGTAGGTACTCAGAATGGTGTTAGAATCAAGACGAAGAGTCCCCCTCAACCAGGAACTGTTGCACAAGTTACATTTGAAAACATTGAAATGGATAAGGTCGATAATCCCATCATCATTGATCAACAATATTGCGCTAGTAGTGGTTGTAGTCCACCGGTAAGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

241

Amino Acids

26.13

Weight (kDa)

5.92

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 239 6.1e-52 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 287
AciI CCGC 2 cut(s) 138, 480
AclI AACGTT 2 cut(s) 462, 471
AcoI YGGCCR 3 cut(s) 199, 339, 487
AfaI GTAC 1 cut(s) 565
AfiI CCNNNNNNNGG 2 cut(s) 491, 608
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 381, 538, 635, 644
AhdI GACNNNNNGTC 1 cut(s) 594
AjnI CCWGG 2 cut(s) 484, 607
AluBI AGCT 4 cut(s) 10, 149, 434, 555
AluI AGCT 4 cut(s) 10, 149, 434, 555
AlwNI CAGNNNCTG 1 cut(s) 614
AoxI GGCC 5 cut(s) 176, 199, 339, 482, 487
ApeKI GCWGC 1 cut(s) 552
AsiGI ACCGGT 1 cut(s) 712
Asp700I GAANNNNTTC 1 cut(s) 511
AspLEI GCGC 1 cut(s) 692
AspS9I GGNCC 1 cut(s) 482
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 97
BaeGI GKGCMC 2 cut(s) 36, 337
BalI TGGCCA 1 cut(s) 201
BbvI GCAGC 1 cut(s) 564
BccI CCATC 3 cut(s) 191, 353, 674
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 2 cut(s) 486, 609
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 1 cut(s) 338
BfaI CTAG 2 cut(s) 171, 693
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 1 cut(s) 553
BlsI GCNGC 1 cut(s) 554
Bme1390I CCNGG 3 cut(s) 338, 486, 609
BmeRI GACNNNNNGTC 1 cut(s) 594
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 3 cut(s) 338, 486, 609
BmsI GCATC 1 cut(s) 393
BplI GAGNNNNNCTC 2 cut(s) 586, 618
BpuMI CCSGG 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 336, 490
BsaWI WCCGGW 2 cut(s) 416, 712
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 608
Bse118I RCCGGY 2 cut(s) 174, 712
Bse1I ACTGG 3 cut(s) 166, 516, 535
BseBI CCWGG 2 cut(s) 486, 609
BseDI CCNNGG 2 cut(s) 336, 490
BseGI GGATG 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 491, 608
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 3 cut(s) 166, 516, 535
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 2 cut(s) 36, 337
BseX3I CGGCCG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 564
BseYI CCCAGC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 73
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 5 cut(s) 178, 201, 341, 484, 489
BshTI ACCGGT 1 cut(s) 712
BsiEI CGRYCG 1 cut(s) 342
BsiSI CCGG 4 cut(s) 175, 338, 417, 713
BslFI GGGAC 1 cut(s) 582
BslI CCNNNNNNNGG 2 cut(s) 491, 608
BsmFI GGGAC 1 cut(s) 582
BsnI GGCC 5 cut(s) 178, 201, 341, 484, 489
Bsp1286I GDGCHC 2 cut(s) 36, 337
Bsp143I GATC 3 cut(s) 12, 243, 676
BspACI CCGC 2 cut(s) 138, 480
BspANI GGCC 5 cut(s) 178, 201, 341, 484, 489
BspCNI CTCAG 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 72
BspMI ACCTGC 1 cut(s) 287
BsrFI RCCGGY 2 cut(s) 174, 712
BsrI ACTGG 3 cut(s) 166, 516, 535
BssAI RCCGGY 2 cut(s) 174, 712
BssECI CCNNGG 2 cut(s) 336, 490
BssMI GATC 3 cut(s) 12, 243, 676
Bst2UI CCWGG 2 cut(s) 486, 609
Bst4CI ACNGT 2 cut(s) 93, 616
Bst6I CTCTTC 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 176, 482
BstDEI CTNAG 1 cut(s) 567
BstDSI CCRYGG 1 cut(s) 490
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 1 cut(s) 282
BstHHI GCGC 1 cut(s) 692
BstKTI GATC 3 cut(s) 15, 246, 679
BstMBI GATC 3 cut(s) 12, 243, 676
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 2 cut(s) 171, 486
BstNI CCWGG 2 cut(s) 486, 609
BstSCI CCNGG 3 cut(s) 336, 484, 607
BstSLI GKGCMC 2 cut(s) 36, 337
BstV1I GCAGC 1 cut(s) 564
BstZI CGGCCG 1 cut(s) 339
BsuRI GGCC 5 cut(s) 178, 201, 341, 484, 489
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 1 cut(s) 282
BtsI GCAGTG 2 cut(s) 61, 475
BtsIMutI CAGTG 4 cut(s) 56, 61, 159, 475
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 2 cut(s) 176, 482
CaiI CAGNNNCTG 1 cut(s) 614
CfoI GCGC 1 cut(s) 692
Cfr10I RCCGGY 2 cut(s) 174, 712
Cfr13I GGNCC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 564
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 1 cut(s) 182
CviQI GTAC 1 cut(s) 564
DdeI CTNAG 1 cut(s) 567
DpnI GATC 3 cut(s) 14, 245, 678
DpnII GATC 3 cut(s) 12, 243, 676
DriI GACNNNNNGTC 1 cut(s) 594
EaeI YGGCCR 3 cut(s) 199, 339, 487
EagI CGGCCG 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 587
Eam1105I GACNNNNNGTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 587
EclXI CGGCCG 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 339
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoRII CCWGG 2 cut(s) 484, 607
FaeI CATG 1 cut(s) 185
FaiI YATR 9 cut(s) 102, 135, 144, 183, 254, 329, 397, 399, 431
FaqI GGGAC 1 cut(s) 582
FatI CATG 1 cut(s) 181
FauI CCCGC 1 cut(s) 473
FbaI TGATCA 1 cut(s) 676
Fnu4HI GCNGC 1 cut(s) 553
FokI GGATG 1 cut(s) 269
Fsp4HI GCNGC 1 cut(s) 553
FspBI CTAG 2 cut(s) 171, 693
GlaI GCGC 1 cut(s) 691
GluI GCNGC 1 cut(s) 553
GsaI CCCAGC 1 cut(s) 39
HaeIII GGCC 5 cut(s) 178, 201, 341, 484, 489
HapII CCGG 4 cut(s) 175, 338, 417, 713
HhaI GCGC 1 cut(s) 692
Hin1II CATG 1 cut(s) 185
Hin6I GCGC 1 cut(s) 690
HinP1I GCGC 1 cut(s) 690
HinfI GANTC 7 cut(s) 22, 156, 363, 401, 446, 582, 595
HpaII CCGG 4 cut(s) 175, 338, 417, 713
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 475, 710
Hpy188I TCNGA 1 cut(s) 570
Hpy188III TCNNGA 3 cut(s) 153, 443, 586
Hpy8I GTNNAC 2 cut(s) 475, 710
HpyAV CCTTC 1 cut(s) 449
HpyCH4III ACNGT 2 cut(s) 93, 616
HpyCH4IV ACGT 3 cut(s) 462, 471, 540
HpyCH4V TGCA 5 cut(s) 54, 104, 429, 552, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 486
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 3 cut(s) 462, 471, 540
Hsp92II CATG 1 cut(s) 185
HspAI GCGC 1 cut(s) 690
KroI GCCGGC 1 cut(s) 174
KroNI GCCGGC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 3 cut(s) 12, 243, 676
LmnI GCTCC 2 cut(s) 7, 70
Lsp1109I GCAGC 1 cut(s) 564
LweI GCATC 1 cut(s) 393
MaeI CTAG 2 cut(s) 171, 693
MaeII ACGT 3 cut(s) 462, 471, 540
MaeIII GTNAC 5 cut(s) 85, 157, 463, 518, 625
MalI GATC 3 cut(s) 14, 245, 678
MboI GATC 3 cut(s) 12, 243, 676
MboII GAAGA 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 36, 337
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 3 cut(s) 495, 547, 721
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 2 cut(s) 165, 604
MnlI CCTC 4 cut(s) 61, 189, 210, 612
Mox20I TGGCCA 1 cut(s) 201
MroNI GCCGGC 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 511
MscI TGGCCA 1 cut(s) 201
MseI TTAA 4 cut(s) 303, 324, 459, 724
MslI CAYNNNNRTG 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 4 cut(s) 175, 338, 417, 713
MspR9I CCNGG 3 cut(s) 338, 486, 609
MvaI CCWGG 2 cut(s) 486, 609
MwoI GCNNNNNNNGC 2 cut(s) 171, 486
NaeI GCCGGC 1 cut(s) 176
NciI CCSGG 1 cut(s) 338
NdeII GATC 3 cut(s) 12, 243, 676
NgoMIV GCCGGC 1 cut(s) 174
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 2 cut(s) 85, 157
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PdiI GCCGGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 511
PfeI GAWTC 5 cut(s) 22, 363, 401, 446, 582
PinAI ACCGGT 1 cut(s) 712
PkrI GCNGC 1 cut(s) 554
PleI GAGTC 2 cut(s) 164, 603
PpsI GAGTC 2 cut(s) 164, 603
Psp1406I AACGTT 2 cut(s) 462, 471
Psp6I CCWGG 2 cut(s) 484, 607
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 2 cut(s) 484, 607
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 482
PstNI CAGNNNCTG 1 cut(s) 614
RsaI GTAC 1 cut(s) 565
RsaNI GTAC 1 cut(s) 564
RseI CAYNNNNRTG 1 cut(s) 644
SaqAI TTAA 4 cut(s) 303, 324, 459, 724
SatI GCNGC 1 cut(s) 553
Sau3AI GATC 3 cut(s) 12, 243, 676
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 2 cut(s) 165, 604
ScrFI CCNGG 3 cut(s) 338, 486, 609
SduI GDGCHC 2 cut(s) 36, 337
SfaNI GCATC 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 644
Sse9I AATT 3 cut(s) 495, 547, 721
SsiI CCGC 2 cut(s) 138, 480
SspI AATATT 1 cut(s) 686
SspMI CTAG 2 cut(s) 171, 693
StyD4I CCNGG 3 cut(s) 336, 484, 607
TaaI ACNGT 2 cut(s) 93, 616
TaiI ACGT 3 cut(s) 465, 474, 543
TaqI TCGA 4 cut(s) 20, 222, 246, 657
TasI AATT 3 cut(s) 495, 547, 721
TfiI GAWTC 5 cut(s) 22, 363, 401, 446, 582
Tru1I TTAA 4 cut(s) 303, 324, 459, 724
Tru9I TTAA 4 cut(s) 303, 324, 459, 724
TscAI CASTG 4 cut(s) 56, 61, 166, 482
TseFI GTSAC 2 cut(s) 85, 157
TseI GCWGC 1 cut(s) 552
Tsp45I GTSAC 2 cut(s) 85, 157
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 4 cut(s) 56, 61, 166, 482
XagI CCTNNNNNAGG 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 511
XspI CTAG 2 cut(s) 171, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.