Rorug05G0197100

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002146
Physical Location & Seq
Forward (+)
19516589 .. 19519149
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0197100.1

Sequence Viewer

Length: 993 bp
ATGTACGGCAGACACAATCCGGACTGGTTTGAAGCGAAGTCATGGGAGTGCGCGATGTACGGTCATTTTAGTGAATATGAGAGGTATGCAAGTATTTTAAAGGACAAAGTAAGGATTTCGCAGTATGAAAGTGGTGTCATTATTCGATATGGAGCGGGGCCTTATAGGGTGAAAGTAAAATTGTCCGAGGTTGAAGTCGGAATTATAGAGGATGTGTATTTAGGAGGGAAAACACTGACAGCAGAAGAAGGAAGATTATGCTTGGGAATTTGTGGAAATGGGGGTAATTCAATTTCCCGCGACGAGATAAATTGTTTGCTACCGAGAACAAATGTCTCAGGAAGTGTGATTAATTATGTGGTGGCGTACCTAAATAAGGAAGGACTCAGCAATTGGTATTGGTCAACGCATTTTGAGGTGCTTATGGCCTTTTATGCTGCTAATGGATTGGGTATGGAAATGCAGATATTTTTGCCTGTGCATGAGGGTTCAATGAAGGATGGCCATTGGATTCTCATTGTGGTTGACATGGTAAAAAAGGTTGCTGAAGTATGGGATAGCGGACCGGGCAAGGTACTTAAGACGTCGAGAATGGATAACATTTCACAAGTGGATGATAAGGTAGAAGAATTTACAATTGTGGAGGCAACAAATGTTCCAAAGCAAGGAAACTCATTTGACTGCGGGGTCTACGTGATACAAAACATGCAGCATTATCGGACCGATTGGGCAAAAGAGTACGACTCAGACACGCAGAGATCGGCCATATTACTGGATTGTTTAACAGGCCCTAATAACGAGTTGGATGCAAATTTACGAAGGAGTATCATAAATAATAAAGATGGGGGTGAGAATGAAGCGACGCCACCTGAAGGTTCCCAGAACAAGCGACAACATCAATTGAGGGAAGAATGGGAAGAAATAGAGGAAGGGCAGTATTGGCGCCTACCTGGAGGAATGCGACCACATCTCAATAATAAGGATGGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

330

Amino Acids

37.49

Weight (kDa)

5.17

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 156 - 240 4.8e-11 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
AatII GACGTC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 942
AccBSI CCGCTC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 690
AccII CGCG 2 cut(s) 53, 300
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 4 cut(s) 155, 298, 561, 684
AcoI YGGCCR 2 cut(s) 502, 762
AcsI RAATTY 3 cut(s) 267, 629, 811
AcuI CTGAAG 2 cut(s) 567, 891
AcyI GRCGYC 3 cut(s) 584, 863, 943
AfaI GTAC 5 cut(s) 5, 59, 368, 576, 740
AfiI CCNNNNNNNGG 3 cut(s) 376, 665, 872
AflII CTTAAG 1 cut(s) 578
AgsI TTSAA 4 cut(s) 32, 194, 291, 492
AjnI CCWGG 1 cut(s) 949
Alw26I GTCTC 1 cut(s) 340
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 5 cut(s) 158, 426, 502, 762, 787
ApeKI GCWGC 2 cut(s) 437, 709
ApoI RAATTY 3 cut(s) 267, 629, 811
AseI ATTAAT 1 cut(s) 351
AspLEI GCGC 2 cut(s) 53, 945
AspS9I GGNCC 4 cut(s) 158, 563, 720, 788
AsuC2I CCSGG 1 cut(s) 567
AsuHPI GGTGA 2 cut(s) 181, 860
AvaII GGWCC 2 cut(s) 563, 720
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 942
BbvI GCAGC 2 cut(s) 424, 721
BccI CCATC 3 cut(s) 494, 836, 977
BceAI ACGGC 1 cut(s) 22
BcgI CGANNNNNNTGC 4 cut(s) 698, 732, 788, 822
BciT130I CCWGG 1 cut(s) 951
BcnI CCSGG 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 340
BfoI RGCGCY 1 cut(s) 946
BfrI CTTAAG 1 cut(s) 578
BisI GCNGC 2 cut(s) 438, 710
BlsI GCNGC 2 cut(s) 439, 711
Bme1390I CCNGG 2 cut(s) 567, 951
Bme18I GGWCC 2 cut(s) 563, 720
BmgT120I GGNCC 4 cut(s) 158, 563, 720, 788
BmiI GGNNCC 3 cut(s) 159, 877, 944
BmrFI CCNGG 2 cut(s) 567, 951
BmsI GCATC 1 cut(s) 796
BplI GAGNNNNNCTC 2 cut(s) 728, 760
BpmI CTGGAG 1 cut(s) 972
BpuMI CCSGG 1 cut(s) 567
BsaAI YACGTR 1 cut(s) 694
BsaHI GRCGYC 3 cut(s) 584, 863, 943
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 665, 872
Bse1I ACTGG 2 cut(s) 29, 777
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 5 cut(s) 217, 505, 619, 811, 988
BseLI CCNNNNNNNGG 3 cut(s) 376, 665, 872
BseMII CTCAG 3 cut(s) 351, 400, 759
BseNI ACTGG 2 cut(s) 29, 777
BseXI GCAGC 2 cut(s) 424, 721
Bsh1236I CGCG 2 cut(s) 53, 300
BshFI GGCC 5 cut(s) 160, 428, 504, 764, 789
BshNI GGYRCC 1 cut(s) 942
BsiSI CCGG 2 cut(s) 20, 566
BslI CCNNNNNNNGG 3 cut(s) 376, 665, 872
BsmAI GTCTC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 963
BsnI GGCC 5 cut(s) 160, 428, 504, 764, 789
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 1 cut(s) 758
BspACI CCGC 4 cut(s) 155, 298, 561, 684
BspANI GGCC 5 cut(s) 160, 428, 504, 764, 789
BspCNI CTCAG 3 cut(s) 350, 399, 758
BspEI TCCGGA 1 cut(s) 19
BspFNI CGCG 2 cut(s) 53, 300
BspLI GGNNCC 3 cut(s) 159, 877, 944
BspT107I GGYRCC 1 cut(s) 942
BspTI CTTAAG 1 cut(s) 578
BsrBI CCGCTC 1 cut(s) 155
BsrI ACTGG 2 cut(s) 29, 777
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 1 cut(s) 758
BssNI GRCGYC 3 cut(s) 584, 863, 943
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 1 cut(s) 62
BstACI GRCGYC 3 cut(s) 584, 863, 943
BstAFI CTTAAG 1 cut(s) 578
BstBAI YACGTR 1 cut(s) 694
BstDEI CTNAG 3 cut(s) 337, 386, 745
BstENI CCTNNNNNAGG 1 cut(s) 374
BstF5I GGATG 5 cut(s) 217, 505, 619, 811, 988
BstFNI CGCG 2 cut(s) 53, 300
BstH2I RGCGCY 1 cut(s) 946
BstHHI GCGC 2 cut(s) 53, 945
BstKTI GATC 1 cut(s) 761
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 1 cut(s) 758
BstMWI GCNNNNNNNGC 3 cut(s) 434, 567, 940
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 1 cut(s) 709
BstSCI CCNGG 2 cut(s) 565, 949
BstUI CGCG 2 cut(s) 53, 300
BstV1I GCAGC 2 cut(s) 424, 721
BstXI CCANNNNNNTGG 1 cut(s) 772
BsuRI GGCC 5 cut(s) 160, 428, 504, 764, 789
BtgZI GCGATG 1 cut(s) 68
BtsCI GGATG 5 cut(s) 217, 505, 619, 811, 988
BtsIMutI CAGTG 1 cut(s) 233
CfoI GCGC 2 cut(s) 53, 945
Cfr13I GGNCC 4 cut(s) 158, 563, 720, 788
CpoI CGGWCCG 2 cut(s) 563, 720
CseI GACGC 1 cut(s) 871
Csp6I GTAC 5 cut(s) 4, 58, 367, 575, 739
CspI CGGWCCG 2 cut(s) 563, 720
CviAII CATG 4 cut(s) 42, 482, 529, 706
CviJI RGCY 5 cut(s) 160, 428, 504, 764, 789
CviKI_1 RGCY 5 cut(s) 160, 428, 504, 764, 789
CviQI GTAC 5 cut(s) 4, 58, 367, 575, 739
DdeI CTNAG 3 cut(s) 337, 386, 745
DinI GGCGCC 1 cut(s) 944
DpnI GATC 1 cut(s) 760
DpnII GATC 1 cut(s) 758
DraI TTTAAA 1 cut(s) 99
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 2 cut(s) 502, 762
Eco47I GGWCC 2 cut(s) 563, 720
Eco57I CTGAAG 2 cut(s) 567, 891
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoO109I RGGNCCY 2 cut(s) 158, 788
EcoRII CCWGG 1 cut(s) 949
EgeI GGCGCC 1 cut(s) 944
EheI GGCGCC 1 cut(s) 944
FaeI CATG 4 cut(s) 45, 485, 532, 709
FatI CATG 4 cut(s) 41, 481, 528, 705
FauI CCCGC 3 cut(s) 148, 305, 677
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 2 cut(s) 438, 710
FokI GGATG 4 cut(s) 224, 512, 626, 818
Fsp4HI GCNGC 2 cut(s) 438, 710
GlaI GCGC 2 cut(s) 52, 944
GluI GCNGC 2 cut(s) 438, 710
GsuI CTGGAG 1 cut(s) 972
HaeII RGCGCY 1 cut(s) 946
HaeIII GGCC 5 cut(s) 160, 428, 504, 764, 789
HapII CCGG 2 cut(s) 20, 566
HgaI GACGC 1 cut(s) 871
HhaI GCGC 2 cut(s) 53, 945
Hin1I GRCGYC 3 cut(s) 584, 863, 943
Hin1II CATG 4 cut(s) 45, 485, 532, 709
Hin6I GCGC 2 cut(s) 51, 943
HinP1I GCGC 2 cut(s) 51, 943
HincII GTYRAC 2 cut(s) 405, 526
HindII GTYRAC 2 cut(s) 405, 526
HinfI GANTC 3 cut(s) 384, 511, 743
HpaII CCGG 2 cut(s) 20, 566
HphI GGTGA 2 cut(s) 181, 860
Hpy166II GTNNAC 3 cut(s) 405, 526, 691
Hpy188I TCNGA 4 cut(s) 187, 200, 720, 748
Hpy188III TCNNGA 3 cut(s) 20, 339, 588
Hpy8I GTNNAC 3 cut(s) 405, 526, 691
Hpy99I CGWCG 3 cut(s) 305, 589, 865
HpyAV CCTTC 6 cut(s) 242, 374, 490, 813, 866, 923
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 2 cut(s) 584, 693
HpyCH4V TGCA 5 cut(s) 89, 463, 481, 709, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 567, 940
HpyF3I CTNAG 3 cut(s) 337, 386, 745
HpySE526I ACGT 2 cut(s) 584, 693
Hsp92I GRCGYC 3 cut(s) 584, 863, 943
Hsp92II CATG 4 cut(s) 45, 485, 532, 709
HspAI GCGC 2 cut(s) 51, 943
KasI GGCGCC 1 cut(s) 942
Kpn2I TCCGGA 1 cut(s) 19
Kzo9I GATC 1 cut(s) 758
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 2 cut(s) 424, 721
LweI GCATC 1 cut(s) 796
MaeII ACGT 2 cut(s) 584, 693
MalI GATC 1 cut(s) 760
MbiI CCGCTC 1 cut(s) 155
MboI GATC 1 cut(s) 758
MboII GAAGA 5 cut(s) 257, 264, 638, 920, 929
MfeI CAATTG 3 cut(s) 391, 636, 899
MlsI TGGCCA 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 504
Mly113I GGCGCC 1 cut(s) 943
MlyI GAGTC 2 cut(s) 378, 737
MmeI TCCRAC 2 cut(s) 178, 783
Mox20I TGGCCA 1 cut(s) 504
MroI TCCGGA 1 cut(s) 19
MscI TGGCCA 1 cut(s) 504
MseI TTAA 4 cut(s) 98, 351, 579, 782
MslI CAYNNNNRTG 2 cut(s) 46, 69
Msp20I TGGCCA 1 cut(s) 504
MspCI CTTAAG 1 cut(s) 578
MspI CCGG 2 cut(s) 20, 566
MspR9I CCNGG 2 cut(s) 567, 951
MunI CAATTG 3 cut(s) 391, 636, 899
Mva1269I GAATGC 1 cut(s) 963
MvaI CCWGG 1 cut(s) 951
MvnI CGCG 2 cut(s) 53, 300
MwoI GCNNNNNNNGC 3 cut(s) 434, 567, 940
NarI GGCGCC 1 cut(s) 943
NciI CCSGG 1 cut(s) 567
NdeII GATC 1 cut(s) 758
NlaIII CATG 4 cut(s) 45, 485, 532, 709
NlaIV GGNNCC 3 cut(s) 159, 877, 944
NspI RCATGY 1 cut(s) 709
PctI GAATGC 1 cut(s) 963
PfeI GAWTC 1 cut(s) 511
PkrI GCNGC 2 cut(s) 439, 711
PleI GAGTC 2 cut(s) 378, 737
PluTI GGCGCC 1 cut(s) 946
PpsI GAGTC 2 cut(s) 378, 737
Ppu21I YACGTR 1 cut(s) 694
PshBI ATTAAT 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 949
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 3 cut(s) 159, 877, 944
PspPI GGNCC 4 cut(s) 158, 563, 720, 788
RsaI GTAC 5 cut(s) 5, 59, 368, 576, 740
RsaNI GTAC 5 cut(s) 4, 58, 367, 575, 739
RseI CAYNNNNRTG 2 cut(s) 46, 69
Rsr2I CGGWCCG 2 cut(s) 563, 720
RsrII CGGWCCG 2 cut(s) 563, 720
SaqAI TTAA 4 cut(s) 98, 351, 579, 782
SatI GCNGC 2 cut(s) 438, 710
Sau3AI GATC 1 cut(s) 758
Sau96I GGNCC 4 cut(s) 158, 563, 720, 788
SchI GAGTC 2 cut(s) 378, 737
ScrFI CCNGG 2 cut(s) 567, 951
SfaNI GCATC 1 cut(s) 796
SfoI GGCGCC 1 cut(s) 944
SinI GGWCC 2 cut(s) 563, 720
SmiMI CAYNNNNRTG 2 cut(s) 46, 69
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SsiI CCGC 4 cut(s) 155, 298, 561, 684
SspDI GGCGCC 1 cut(s) 942
StyD4I CCNGG 2 cut(s) 565, 949
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 2 cut(s) 587, 696
TaqI TCGA 2 cut(s) 145, 587
TaqII GACCGA 1 cut(s) 737
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 4 cut(s) 98, 351, 579, 782
Tru9I TTAA 4 cut(s) 98, 351, 579, 782
TscAI CASTG 1 cut(s) 240
TseI GCWGC 2 cut(s) 437, 709
TspDTI ATGAA 3 cut(s) 141, 509, 870
TspRI CASTG 1 cut(s) 240
Vha464I CTTAAG 1 cut(s) 578
VpaK11BI GGWCC 2 cut(s) 563, 720
VspI ATTAAT 1 cut(s) 351
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 3 cut(s) 267, 629, 811
XceI RCATGY 1 cut(s) 709
XmiI GTMKAC 1 cut(s) 690
ZraI GACGTC 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.