Rw2G010740

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002146
Physical Location & Seq
Forward (+)
10893184 .. 10895443
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010740.1

Sequence Viewer

Length: 1242 bp
ATGGGCTTAAGCTTTTTCATCCAATGCGCTTGCTTAGTGTCGTTGCTTGCCTTCATTAATGCATGGAATGGAGCATGTCAGAACATTGAAGGAGGTATGGTGCTGTTCCCAAGAGGCACATTTGTGGTGGCTCCGGTTGTTCTTCCAGGTCGATGCAAGGGACCAATGGAGTGGGAAATTCAGGGTACCCTACAGGCTTCTAAGGAGGTCAAAGATTTCATTGGTATTGACCATTGGATCACCATACAATACGTCGACGGCTTGAACATTAGTGGTGGTGGAACCCTAGATGGCCTCGGAGCCGATGGTTGGGGATATAATGACTGCCTAAAAAACCCAGGCCGTTGCAAGCAACTCCCTGCTACACTGCGGTTGGATTTTGTCACCAAAGGTTACATAACCAACATAACATCGATCAACAGCAAAAACACCCACATCAATCTTTTTGCATGCAAGGAAGTACTTATTCGCAACATCAAGATATCAGCTCCCGGAGAGAGTCCCAACACCGATGGAATCCACATTGGCAACTCACAGAACATCTATATCGTAGACTCGCAGATATCCACCGGTGACGATTGTGTCTCCGTCCTCCCCGGATCACAATACATCAATGTCACCGGTGTCCAGTGCGGTCCAGGCCATGGAATTAGCATTGGAAGTCTCGGCAGAGGTGCTGGTGACGCCGTTAATGACATGTATGTAACAAACAGCAGATTTGTGAGTACTCAAAATGGTGTGAGAATCAAAGCATGGGCTGTGGATTCTAAACCTGGAACTGTATCACGTATTGCCTTTGGGTACATTGAAATGGATAATGTGGAAAATCCCATCATTATTGATCAAGAATATTGCCCTGGTGGTGGTTGTAATACACAGGCATCCTCCCAAGTTCAGATTAGTGATATAAAGTTTAATGACATTAGAGGCACTTCGGCTACAAAGGAAGCAATTATTCTCAAATGCAGCCGAAGTAAACCTTGCCAAAATATCGAGCTGAAGGACATTGACCTAACCTTCCGAGGACCTGGAGGGCCGGCAGTTTCATTGTGCAAAAATGTAGAAGGCAGAGCTTACGGCCTACAAAAGCCCACATCCAAATTAGATACACCGCGAGTAGCTGCTTACAATGAAATAGAGTATGATAACTTCGAAGGCACAGCTTATGATCTACAGAACCCCTCCTGGAATCTAAACACGGTGCAGGAATCCCCTGACCGCGAAATAGAGGATCCTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

413

Amino Acids

44.61

Weight (kDa)

5.09

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 26 - 355 1.9e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 2 cut(s) 255, 552
AccII CGCG 2 cut(s) 1114, 1221
AciI CCGC 4 cut(s) 370, 633, 1112, 1219
AclWI GGATC 4 cut(s) 245, 607, 1226, 1239
AcsI RAATTY 1 cut(s) 177
AcuI CTGAAG 1 cut(s) 1019
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 4 cut(s) 187, 462, 727, 803
AfiI CCNNNNNNNGG 3 cut(s) 309, 644, 863
AflII CTTAAG 1 cut(s) 7
AflIII ACRYGT 1 cut(s) 696
AgeI ACCGGT 2 cut(s) 569, 620
AgsI TTSAA 3 cut(s) 89, 265, 809
AjnI CCWGG 7 cut(s) 145, 337, 637, 772, 856, 1027, 1184
AleI CACNNNNGTG 1 cut(s) 122
AluBI AGCT 6 cut(s) 12, 488, 997, 1073, 1121, 1163
AluI AGCT 6 cut(s) 12, 488, 997, 1073, 1121, 1163
Alw26I GTCTC 2 cut(s) 589, 668
AlwI GGATC 4 cut(s) 245, 607, 1226, 1239
AoxI GGCC 5 cut(s) 292, 340, 640, 1034, 1078
ApeKI GCWGC 2 cut(s) 966, 1121
ApoI RAATTY 1 cut(s) 177
AseI ATTAAT 1 cut(s) 57
AsiGI ACCGGT 2 cut(s) 569, 620
Asp718I GGTACC 1 cut(s) 185
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 4 cut(s) 161, 635, 1025, 1034
AsuC2I CCSGG 2 cut(s) 492, 597
AsuHPI GGTGA 5 cut(s) 232, 376, 584, 610, 692
AsuII TTCGAA 1 cut(s) 1152
AvaII GGWCC 3 cut(s) 161, 635, 1025
BamHI GGATCC 1 cut(s) 1231
BanI GGYRCC 1 cut(s) 185
BarI GAAGNNNNNNTAC 2 cut(s) 1133, 1165
BbvI GCAGC 2 cut(s) 978, 1108
BccI CCATC 4 cut(s) 284, 299, 506, 839
BceAI ACGGC 4 cut(s) 274, 327, 671, 1093
BciT130I CCWGG 7 cut(s) 147, 339, 639, 774, 858, 1029, 1186
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 2 cut(s) 492, 597
BcoDI GTCTC 2 cut(s) 589, 668
BfaI CTAG 1 cut(s) 287
BfmI CTRYAG 2 cut(s) 191, 1172
BfrI CTTAAG 1 cut(s) 7
BisI GCNGC 2 cut(s) 967, 1122
BlsI GCNGC 2 cut(s) 968, 1123
BmcAI AGTACT 2 cut(s) 462, 727
Bme1390I CCNGG 9 cut(s) 147, 339, 492, 597, 639, 774, 858, 1029, 1186
Bme18I GGWCC 3 cut(s) 161, 635, 1025
BmgT120I GGNCC 4 cut(s) 161, 635, 1025, 1034
BmiI GGNNCC 6 cut(s) 132, 162, 187, 283, 301, 1233
BmrFI CCNGG 9 cut(s) 147, 339, 492, 597, 639, 774, 858, 1029, 1186
BmsI GCATC 2 cut(s) 143, 890
BpmI CTGGAG 1 cut(s) 1050
Bpu14I TTCGAA 1 cut(s) 1152
BpuMI CCSGG 2 cut(s) 492, 597
Bsa29I ATCGAT 1 cut(s) 413
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 684
BsaJI CCNNGG 6 cut(s) 295, 337, 595, 643, 856, 1021
BsaWI WCCGGW 3 cut(s) 133, 569, 620
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 644, 863
Bse118I RCCGGY 3 cut(s) 569, 620, 1036
Bse1I ACTGG 1 cut(s) 628
BseBI CCWGG 7 cut(s) 147, 339, 639, 774, 858, 1029, 1186
BseCI ATCGAT 1 cut(s) 413
BseDI CCNNGG 6 cut(s) 295, 337, 595, 643, 856, 1021
BseGI GGATG 3 cut(s) 18, 881, 1094
BseLI CCNNNNNNNGG 3 cut(s) 309, 644, 863
BseNI ACTGG 1 cut(s) 628
BseXI GCAGC 2 cut(s) 978, 1108
BsgI GTGCAG 1 cut(s) 1223
Bsh1236I CGCG 2 cut(s) 1114, 1221
BshFI GGCC 5 cut(s) 294, 342, 642, 1036, 1080
BshNI GGYRCC 1 cut(s) 185
BshTI ACCGGT 2 cut(s) 569, 620
BshVI ATCGAT 1 cut(s) 413
BsiSI CCGG 6 cut(s) 134, 492, 570, 597, 621, 1037
BslFI GGGAC 2 cut(s) 174, 486
BslI CCNNNNNNNGG 3 cut(s) 309, 644, 863
BsmAI GTCTC 2 cut(s) 589, 668
BsmFI GGGAC 2 cut(s) 174, 486
BsnI GGCC 5 cut(s) 294, 342, 642, 1036, 1080
Bsp119I TTCGAA 1 cut(s) 1152
Bsp143I GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 4 cut(s) 370, 633, 1112, 1219
BspANI GGCC 5 cut(s) 294, 342, 642, 1036, 1080
BspDI ATCGAT 1 cut(s) 413
BspFNI CGCG 2 cut(s) 1114, 1221
BspLI GGNNCC 6 cut(s) 132, 162, 187, 283, 301, 1233
BspPI GGATC 4 cut(s) 245, 607, 1226, 1239
BspT104I TTCGAA 1 cut(s) 1152
BspT107I GGYRCC 1 cut(s) 185
BspTI CTTAAG 1 cut(s) 7
BsrFI RCCGGY 3 cut(s) 569, 620, 1036
BsrI ACTGG 1 cut(s) 628
BssAI RCCGGY 3 cut(s) 569, 620, 1036
BssECI CCNNGG 6 cut(s) 295, 337, 595, 643, 856, 1021
BssMI GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
BssNI GRCGYC 1 cut(s) 684
BssT1I CCWWGG 1 cut(s) 643
Bst2UI CCWGG 7 cut(s) 147, 339, 639, 774, 858, 1029, 1186
Bst4CI ACNGT 2 cut(s) 781, 1201
BstACI GRCGYC 1 cut(s) 684
BstAFI CTTAAG 1 cut(s) 7
BstBAI YACGTR 1 cut(s) 788
BstBI TTCGAA 1 cut(s) 1152
BstC8I GCNNGC 5 cut(s) 31, 48, 350, 451, 1038
BstDEI CTNAG 2 cut(s) 34, 201
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 3 cut(s) 18, 881, 1094
BstFNI CGCG 2 cut(s) 1114, 1221
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 6 cut(s) 240, 417, 602, 844, 1171, 1234
BstMAI GTCTC 2 cut(s) 589, 668
BstMBI GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 639, 683
BstNI CCWGG 7 cut(s) 147, 339, 639, 774, 858, 1029, 1186
BstNSI RCATGY 3 cut(s) 78, 453, 700
BstSCI CCNGG 9 cut(s) 145, 337, 490, 595, 637, 772, 856, 1027, 1184
BstSFI CTRYAG 2 cut(s) 191, 1172
BstUI CGCG 2 cut(s) 1114, 1221
BstV1I GCAGC 2 cut(s) 978, 1108
BstX2I RGATCY 1 cut(s) 1231
BstXI CCANNNNNNTGG 1 cut(s) 171
BstYI RGATCY 1 cut(s) 1231
Bsu15I ATCGAT 1 cut(s) 413
BsuRI GGCC 5 cut(s) 294, 342, 642, 1036, 1080
BsuTUI ATCGAT 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 643
BtsCI GGATG 3 cut(s) 18, 881, 1094
BtsI GCAGTG 1 cut(s) 365
BtsIMutI CAGTG 2 cut(s) 365, 635
Cac8I GCNNGC 5 cut(s) 31, 48, 350, 451, 1038
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 3 cut(s) 569, 620, 1036
Cfr13I GGNCC 4 cut(s) 161, 635, 1025, 1034
ClaI ATCGAT 1 cut(s) 413
CseI GACGC 1 cut(s) 692
Csp6I GTAC 4 cut(s) 186, 461, 726, 802
CspAI ACCGGT 2 cut(s) 569, 620
CviAII CATG 6 cut(s) 63, 75, 450, 644, 697, 753
CviQI GTAC 4 cut(s) 186, 461, 726, 802
DdeI CTNAG 2 cut(s) 34, 201
DpnI GATC 6 cut(s) 239, 416, 601, 843, 1170, 1233
DpnII GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
Eco130I CCWWGG 1 cut(s) 643
Eco32I GATATC 2 cut(s) 483, 564
Eco47I GGWCC 3 cut(s) 161, 635, 1025
Eco57I CTGAAG 1 cut(s) 1019
EcoO109I RGGNCCY 1 cut(s) 1025
EcoRII CCWGG 7 cut(s) 145, 337, 637, 772, 856, 1027, 1184
EcoRV GATATC 2 cut(s) 483, 564
EcoT14I CCWWGG 1 cut(s) 643
EcoT22I ATGCAT 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 643
FaeI CATG 6 cut(s) 66, 78, 453, 647, 700, 756
FalI AAGNNNNNCTT 2 cut(s) 964, 996
FaqI GGGAC 2 cut(s) 174, 486
FatI CATG 6 cut(s) 62, 74, 449, 643, 696, 752
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 2 cut(s) 255, 552
Fnu4HI GCNGC 2 cut(s) 967, 1122
FokI GGATG 3 cut(s) 5, 868, 1081
Fsp4HI GCNGC 2 cut(s) 967, 1122
FspBI CTAG 1 cut(s) 287
GlaI GCGC 1 cut(s) 28
GluI GCNGC 2 cut(s) 967, 1122
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 5 cut(s) 294, 342, 642, 1036, 1080
HapII CCGG 6 cut(s) 134, 492, 570, 597, 621, 1037
HgaI GACGC 1 cut(s) 692
HhaI GCGC 1 cut(s) 29
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 6 cut(s) 66, 78, 453, 647, 700, 756
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 7 cut(s) 499, 516, 554, 744, 764, 1189, 1208
HpaII CCGG 6 cut(s) 134, 492, 570, 597, 621, 1037
HphI GGTGA 5 cut(s) 232, 376, 584, 610, 692
Hpy166II GTNNAC 3 cut(s) 256, 553, 977
Hpy188I TCNGA 4 cut(s) 81, 299, 897, 1022
Hpy188III TCNNGA 2 cut(s) 478, 845
Hpy8I GTNNAC 3 cut(s) 256, 553, 977
Hpy99I CGWCG 2 cut(s) 257, 260
HpyAV CCTTC 6 cut(s) 61, 83, 994, 1027, 1058, 1148
HpyCH4III ACNGT 2 cut(s) 781, 1201
HpyCH4IV ACGT 2 cut(s) 252, 787
HpyCH4V TGCA 8 cut(s) 62, 156, 348, 449, 453, 966, 1053, 1204
HpyF10VI GCNNNNNNNGC 2 cut(s) 639, 683
HpyF3I CTNAG 2 cut(s) 34, 201
HpySE526I ACGT 2 cut(s) 252, 787
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 6 cut(s) 66, 78, 453, 647, 700, 756
HspAI GCGC 1 cut(s) 27
KpnI GGTACC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 1036
KroNI GCCGGC 1 cut(s) 1038
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
LmnI GCTCC 4 cut(s) 71, 136, 299, 493
Lsp1109I GCAGC 2 cut(s) 978, 1108
LweI GCATC 2 cut(s) 143, 890
MaeI CTAG 1 cut(s) 287
MaeII ACGT 2 cut(s) 252, 787
MaeIII GTNAC 6 cut(s) 382, 392, 572, 616, 680, 703
MalI GATC 6 cut(s) 239, 416, 601, 843, 1170, 1233
MboI GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
MboII GAAGA 1 cut(s) 134
MflI RGATCY 1 cut(s) 1231
MluCI AATT 4 cut(s) 177, 648, 951, 1100
MlyI GAGTC 2 cut(s) 508, 548
MmeI TCCRAC 1 cut(s) 354
Mph1103I ATGCAT 1 cut(s) 64
MroNI GCCGGC 1 cut(s) 1036
MseI TTAA 4 cut(s) 8, 57, 690, 915
MslI CAYNNNNRTG 2 cut(s) 122, 809
MspCI CTTAAG 1 cut(s) 7
MspI CCGG 6 cut(s) 134, 492, 570, 597, 621, 1037
MspR9I CCNGG 9 cut(s) 147, 339, 492, 597, 639, 774, 858, 1029, 1186
MvaI CCWGG 7 cut(s) 147, 339, 639, 774, 858, 1029, 1186
MvnI CGCG 2 cut(s) 1114, 1221
MwoI GCNNNNNNNGC 2 cut(s) 639, 683
NaeI GCCGGC 1 cut(s) 1038
NciI CCSGG 2 cut(s) 492, 597
NcoI CCATGG 1 cut(s) 643
NdeII GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
NgoMIV GCCGGC 1 cut(s) 1036
NlaIII CATG 6 cut(s) 66, 78, 453, 647, 700, 756
NlaIV GGNNCC 6 cut(s) 132, 162, 187, 283, 301, 1233
NmeAIII GCCGAG 1 cut(s) 645
NmuCI GTSAC 4 cut(s) 382, 572, 616, 680
NsiI ATGCAT 1 cut(s) 64
NspI RCATGY 3 cut(s) 78, 453, 700
NspV TTCGAA 1 cut(s) 1152
OliI CACNNNNGTG 1 cut(s) 122
PaeI GCATGC 1 cut(s) 453
PciI ACATGT 1 cut(s) 696
PdiI GCCGGC 1 cut(s) 1038
PfeI GAWTC 5 cut(s) 516, 744, 764, 1189, 1208
PflMI CCANNNNNTGG 1 cut(s) 644
PfoI TCCNGGA 2 cut(s) 490, 1184
PinAI ACCGGT 2 cut(s) 569, 620
PkrI GCNGC 2 cut(s) 968, 1123
PleI GAGTC 2 cut(s) 507, 548
PpsI GAGTC 2 cut(s) 507, 548
Ppu21I YACGTR 1 cut(s) 788
PpuMI RGGWCCY 1 cut(s) 1025
PscI ACATGT 1 cut(s) 696
PshBI ATTAAT 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 1025
Psp6I CCWGG 7 cut(s) 145, 337, 637, 772, 856, 1027, 1184
PspGI CCWGG 7 cut(s) 145, 337, 637, 772, 856, 1027, 1184
PspN4I GGNNCC 6 cut(s) 132, 162, 187, 283, 301, 1233
PspPI GGNCC 4 cut(s) 161, 635, 1025, 1034
PspPPI RGGWCCY 1 cut(s) 1025
PsuI RGATCY 1 cut(s) 1231
RsaI GTAC 4 cut(s) 187, 462, 727, 803
RsaNI GTAC 4 cut(s) 186, 461, 726, 802
RseI CAYNNNNRTG 2 cut(s) 122, 809
SalI GTCGAC 1 cut(s) 254
SaqAI TTAA 4 cut(s) 8, 57, 690, 915
SatI GCNGC 2 cut(s) 967, 1122
Sau3AI GATC 6 cut(s) 237, 414, 599, 841, 1168, 1231
Sau96I GGNCC 4 cut(s) 161, 635, 1025, 1034
ScaI AGTACT 2 cut(s) 462, 727
SchI GAGTC 2 cut(s) 508, 548
ScrFI CCNGG 9 cut(s) 147, 339, 492, 597, 639, 774, 858, 1029, 1186
SfaNI GCATC 2 cut(s) 143, 890
SfcI CTRYAG 2 cut(s) 191, 1172
SfuI TTCGAA 1 cut(s) 1152
SgrAI CRCCGGYG 2 cut(s) 569, 620
SgrDI CGTCGACG 1 cut(s) 254
SinI GGWCC 3 cut(s) 161, 635, 1025
SmiMI CAYNNNNRTG 2 cut(s) 122, 809
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
SphI GCATGC 1 cut(s) 453
Sse9I AATT 4 cut(s) 177, 648, 951, 1100
SsiI CCGC 4 cut(s) 370, 633, 1112, 1219
SspI AATATT 1 cut(s) 851
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 9 cut(s) 145, 337, 490, 595, 637, 772, 856, 1027, 1184
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 2 cut(s) 781, 1201
TaiI ACGT 2 cut(s) 255, 790
TaqI TCGA 5 cut(s) 151, 255, 413, 993, 1152
TasI AATT 4 cut(s) 177, 648, 951, 1100
TatI WGTACW 2 cut(s) 460, 725
TfiI GAWTC 5 cut(s) 516, 744, 764, 1189, 1208
Tru1I TTAA 4 cut(s) 8, 57, 690, 915
Tru9I TTAA 4 cut(s) 8, 57, 690, 915
TscAI CASTG 2 cut(s) 372, 635
TseFI GTSAC 4 cut(s) 382, 572, 616, 680
TseI GCWGC 2 cut(s) 966, 1121
Tsp45I GTSAC 4 cut(s) 382, 572, 616, 680
TspDTI ATGAA 5 cut(s) 7, 43, 208, 1035, 1146
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 2 cut(s) 372, 635
Van91I CCANNNNNTGG 1 cut(s) 644
Vha464I CTTAAG 1 cut(s) 7
VpaK11BI GGWCC 3 cut(s) 161, 635, 1025
VspI ATTAAT 1 cut(s) 57
XapI RAATTY 1 cut(s) 177
XceI RCATGY 3 cut(s) 78, 453, 700
XmiI GTMKAC 2 cut(s) 255, 552
XspI CTAG 1 cut(s) 287
ZrmI AGTACT 2 cut(s) 462, 727
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.