FvH4_1g18510

branched-chain-amino-acid aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004977
Physical Location & Seq
Reverse (-)
10832604 .. 10837080
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18510.t1

Sequence Viewer

Length: 1266 bp
ATGGAGAGCGCCAGCGCCGTCCTTGGGAGCCTTCAGCCAAATTACCTTCTTACCCCTTCCCGCAATCCTTCTTCCTCCTCCTCCCTCCTCCGCCCCTTCTCCGCCTACTATTCCTCTCTCTCTCCCTCTCTCAAGCTACAAAAGCAGCTCCCTTTGGCTTGTTACCAACCGGTTCCTCCCTCCCCTATTCGCCGATGTGCCACATTGTCTGATACTCTCCGCACTGAAACAAGTGAAACAGCTGACATAGAATGGGACAATCTTGGCTTTGGGTTTCTTCCTACCGATTATATGTATGTCATGAAGTGTGCTCAAGGCGGGAGCTTCTCTGATGGTGAATTGCAGCGTTTTGGGAACATTGAGTTGAACCCTTCGGCTGGAGTCTTGAATTATGGCCAGGGTTTGTTTGAAGGTATGAAAGCCTACAGGAAACAAGATGGTAAGATACTCTTGTTTCGACCCGAAGAAAATGCATCGCGGATGAGGTTGGGTGCTGAGCGGATGTGCATGCCCTCACCGACTGTTGAACAATTTGTGGAAGCTGTGAAGGCCACTGTTTTGGCAAATAAACGTTGGGTTCCCCCTCCAGGCAAAGGTTCTCTATATATCAGGCCATTGCTAATGGGAAGTGGTGCTGTTCTTGGTCTTGCTCCTGCTCCTGAATACACATTTCTGATCTATGTTTCACCAGTTGGAAATTACTTTAAGGAAGGCGTCGCACCTATAAACTTGATTGTCGAGCATGAATTGCATCGTGCAACTCCTGGTGGTACTGGAGGTGTCAAAACTATAGGGAATTATGCCGCGGTTTTGAAGGCACAGTGTGCTGCAAAAGCGAAAGGCTACTCGGATGTTCTGTACCTTGACTGCGTTCACAAAAAATATTTAGAGGAGGTTTCCTCATGCAACATATTTGTTGTGAAGGGTGATGTTATCTCTACTCCTGCAATAAAAGGGACGATCTTACCTGGCATTACTCGAAAGAGTATTATTGATGTTGCCCGTAGCCAAGGATTCCAGGTTGAGGAGCGCGCTGTGGATGTAGATGAATTACTTGAAGCTGATGAAGTCTTTTGTACCGGAACCGCTGTTGTCGTGTCACCTGTGGGCAGCATTACTTATATGGATAAAAGGGTGTCATATGGAGAGAGTGGCTTTGGTGCTGTGTCACAGAAGCTCTATTCTGTTCTTACCAGATTACAGATGGGGCTTATAGAGGACAAGATGGATTGGACTGTTGAGCTAAACCCAGCAAAAGCATACTGA

Protein Analysis

422

Amino Acids

45.81

Weight (kDa)

6.84

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 134 - 372 6.7e-38 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 499
AccII CGCG 3 cut(s) 478, 806, 1032
AclI AACGTT 1 cut(s) 571
AcoI YGGCCR 1 cut(s) 394
AcuI CTGAAG 1 cut(s) 17
AcyI GRCGYC 1 cut(s) 714
AdeI CACNNNGTG 1 cut(s) 824
AfaI GTAC 3 cut(s) 772, 860, 1078
AfiI CCNNNNNNNGG 5 cut(s) 24, 377, 587, 593, 1024
AgeI ACCGGT 1 cut(s) 169
AgsI TTSAA 6 cut(s) 367, 388, 410, 527, 814, 1058
AjnI CCWGG 5 cut(s) 396, 586, 763, 967, 1017
AluBI AGCT 8 cut(s) 136, 148, 242, 324, 542, 1061, 1177, 1243
AluI AGCT 8 cut(s) 136, 148, 242, 324, 542, 1061, 1177, 1243
Alw21I GWGCWC 1 cut(s) 313
AoxI GGCC 3 cut(s) 394, 549, 611
ApeKI GCWGC 4 cut(s) 145, 343, 827, 1110
AsiGI ACCGGT 1 cut(s) 169
AspLEI GCGC 4 cut(s) 11, 17, 1032, 1034
AsuHPI GGTGA 5 cut(s) 347, 507, 678, 938, 1092
BaeI ACNNNNGTAYC 2 cut(s) 437, 470
BalI TGGCCA 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 157, 355, 814, 1122
BccI CCATC 4 cut(s) 326, 431, 1198, 1219
BceAI ACGGC 1 cut(s) 2
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 5 cut(s) 398, 588, 765, 969, 1019
BfmI CTRYAG 2 cut(s) 424, 789
BfoI RGCGCY 2 cut(s) 12, 18
BisI GCNGC 5 cut(s) 146, 344, 804, 828, 1111
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 5 cut(s) 147, 345, 805, 829, 1112
Bme1390I CCNGG 5 cut(s) 398, 588, 765, 969, 1019
BmiI GGNNCC 4 cut(s) 29, 174, 579, 1084
BmrFI CCNGG 5 cut(s) 398, 588, 765, 969, 1019
BmsI GCATC 2 cut(s) 482, 760
BplI GAGNNNNNCTC 2 cut(s) 884, 916
BpmI CTGGAG 3 cut(s) 399, 570, 795
Bpu1102I GCTNAGC 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 116, 297
BsaHI GRCGYC 1 cut(s) 714
BsaJI CCNNGG 4 cut(s) 22, 397, 804, 1009
BsaWI WCCGGW 2 cut(s) 169, 1079
Bsc4I CCNNNNNNNGG 5 cut(s) 24, 377, 587, 593, 1024
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 689, 778
Bse3DI GCAATG 1 cut(s) 614
BseBI CCWGG 5 cut(s) 398, 588, 765, 969, 1019
BseDI CCNNGG 4 cut(s) 22, 397, 804, 1009
BseGI GGATG 4 cut(s) 486, 507, 856, 1045
BseLI CCNNNNNNNGG 5 cut(s) 24, 377, 587, 593, 1024
BseMI GCAATG 1 cut(s) 614
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 2 cut(s) 689, 778
BsePI GCGCGC 1 cut(s) 1030
BseRI GAGGAG 5 cut(s) 67, 70, 77, 905, 1040
BseXI GCAGC 4 cut(s) 157, 355, 814, 1122
BseYI CCCAGC 1 cut(s) 1249
Bsh1236I CGCG 3 cut(s) 478, 806, 1032
BshFI GGCC 3 cut(s) 396, 551, 613
BshTI ACCGGT 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 2 cut(s) 170, 1080
BslFI GGGAC 2 cut(s) 269, 970
BslI CCNNNNNNNGG 5 cut(s) 24, 377, 587, 593, 1024
BsmFI GGGAC 2 cut(s) 269, 970
BsnI GGCC 3 cut(s) 396, 551, 613
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 2 cut(s) 675, 960
Bsp1720I GCTNAGC 1 cut(s) 495
BspANI GGCC 3 cut(s) 396, 551, 613
BspCNI CTCAG 1 cut(s) 487
BspFNI CGCG 3 cut(s) 478, 806, 1032
BspHI TCATGA 1 cut(s) 300
BspLI GGNNCC 4 cut(s) 29, 174, 579, 1084
BsrBI CCGCTC 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 689, 778
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 4 cut(s) 22, 397, 804, 1009
BssHII GCGCGC 1 cut(s) 1030
BssMI GATC 2 cut(s) 675, 960
BssNI GRCGYC 1 cut(s) 714
BssT1I CCWWGG 2 cut(s) 22, 1009
Bst2UI CCWGG 5 cut(s) 398, 588, 765, 969, 1019
Bst4CI ACNGT 4 cut(s) 523, 556, 822, 1237
BstACI GRCGYC 1 cut(s) 714
BstAPI GCANNNNNTGC 2 cut(s) 748, 824
BstC8I GCNNGC 3 cut(s) 13, 509, 1032
BstDEI CTNAG 1 cut(s) 495
BstDSI CCRYGG 1 cut(s) 804
BstF5I GGATG 4 cut(s) 486, 507, 856, 1045
BstFNI CGCG 3 cut(s) 478, 806, 1032
BstH2I RGCGCY 2 cut(s) 12, 18
BstHHI GCGC 4 cut(s) 11, 17, 1032, 1034
BstKTI GATC 2 cut(s) 678, 963
BstMBI GATC 2 cut(s) 675, 960
BstMWI GCNNNNNNNGC 5 cut(s) 142, 548, 748, 824, 833
BstNI CCWGG 5 cut(s) 398, 588, 765, 969, 1019
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 5 cut(s) 396, 586, 763, 967, 1017
BstSFI CTRYAG 2 cut(s) 424, 789
BstUI CGCG 3 cut(s) 478, 806, 1032
BstV1I GCAGC 4 cut(s) 157, 355, 814, 1122
BstXI CCANNNNNNTGG 1 cut(s) 559
BsuRI GGCC 3 cut(s) 396, 551, 613
BtgI CCRYGG 1 cut(s) 804
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 486, 507, 856, 1045
BtsIMutI CAGTG 3 cut(s) 222, 552, 827
Cac8I GCNNGC 3 cut(s) 13, 509, 1032
CciI TCATGA 1 cut(s) 300
CfoI GCGC 4 cut(s) 11, 17, 1032, 1034
Cfr10I RCCGGY 1 cut(s) 169
Cfr42I CCGCGG 1 cut(s) 807
CseI GACGC 1 cut(s) 703
Csp6I GTAC 3 cut(s) 771, 859, 1077
CspAI ACCGGT 1 cut(s) 169
CviAII CATG 4 cut(s) 301, 508, 743, 903
CviQI GTAC 3 cut(s) 771, 859, 1077
DdeI CTNAG 1 cut(s) 495
DpnI GATC 2 cut(s) 677, 962
DpnII GATC 2 cut(s) 675, 960
DraIII CACNNNGTG 1 cut(s) 824
EaeI YGGCCR 1 cut(s) 394
EciI GGCGGA 2 cut(s) 80, 91
Eco130I CCWWGG 2 cut(s) 22, 1009
Eco57I CTGAAG 1 cut(s) 17
EcoRII CCWGG 5 cut(s) 396, 586, 763, 967, 1017
EcoT14I CCWWGG 2 cut(s) 22, 1009
EcoT22I ATGCAT 1 cut(s) 475
ErhI CCWWGG 2 cut(s) 22, 1009
FaeI CATG 4 cut(s) 304, 511, 746, 906
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 2 cut(s) 269, 970
FatI CATG 4 cut(s) 300, 507, 742, 902
FauI CCCGC 2 cut(s) 68, 311
FauNDI CATATG 1 cut(s) 1141
Fnu4HI GCNGC 5 cut(s) 146, 344, 804, 828, 1111
FokI GGATG 4 cut(s) 493, 514, 863, 1052
Fsp4HI GCNGC 5 cut(s) 146, 344, 804, 828, 1111
GlaI GCGC 4 cut(s) 10, 16, 1031, 1033
GluI GCNGC 5 cut(s) 146, 344, 804, 828, 1111
GsaI CCCAGC 1 cut(s) 1253
GsuI CTGGAG 3 cut(s) 399, 570, 795
HaeII RGCGCY 2 cut(s) 12, 18
HaeIII GGCC 3 cut(s) 396, 551, 613
HapII CCGG 2 cut(s) 170, 1080
HgaI GACGC 1 cut(s) 703
HhaI GCGC 4 cut(s) 11, 17, 1032, 1034
Hin1I GRCGYC 1 cut(s) 714
Hin1II CATG 4 cut(s) 304, 511, 746, 906
Hin6I GCGC 4 cut(s) 9, 15, 1030, 1032
HinP1I GCGC 4 cut(s) 9, 15, 1030, 1032
HinfI GANTC 2 cut(s) 381, 1014
HpaII CCGG 2 cut(s) 170, 1080
HphI GGTGA 5 cut(s) 347, 507, 678, 938, 1092
Hpy166II GTNNAC 1 cut(s) 874
Hpy188I TCNGA 4 cut(s) 211, 331, 675, 850
Hpy188III TCNNGA 3 cut(s) 301, 385, 659
Hpy8I GTNNAC 1 cut(s) 874
Hpy99I CGWCG 1 cut(s) 719
HpyCH4III ACNGT 4 cut(s) 523, 556, 822, 1237
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 8 cut(s) 343, 473, 507, 751, 758, 830, 906, 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 548, 748, 824, 833
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 1 cut(s) 571
Hsp92I GRCGYC 1 cut(s) 714
Hsp92II CATG 4 cut(s) 304, 511, 746, 906
HspAI GCGC 4 cut(s) 9, 15, 1030, 1032
KspI CCGCGG 1 cut(s) 807
Kzo9I GATC 2 cut(s) 675, 960
LmnI GCTCC 6 cut(s) 27, 153, 321, 655, 661, 1027
Lsp1109I GCAGC 4 cut(s) 157, 355, 814, 1122
LweI GCATC 2 cut(s) 482, 760
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 3 cut(s) 161, 1098, 1167
MalI GATC 2 cut(s) 677, 962
MbiI CCGCTC 1 cut(s) 499
MboI GATC 2 cut(s) 675, 960
MboII GAAGA 3 cut(s) 63, 269, 476
MhlI GDGCHC 1 cut(s) 313
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 8 cut(s) 40, 338, 388, 530, 697, 746, 796, 1049
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 1 cut(s) 390
MmeI TCCRAC 1 cut(s) 673
Mox20I TGGCCA 1 cut(s) 396
Mph1103I ATGCAT 1 cut(s) 475
MscI TGGCCA 1 cut(s) 396
MseI TTAA 1 cut(s) 705
Msp20I TGGCCA 1 cut(s) 396
MspA1I CMGCKG 3 cut(s) 242, 806, 1088
MspI CCGG 2 cut(s) 170, 1080
MspR9I CCNGG 5 cut(s) 398, 588, 765, 969, 1019
MvaI CCWGG 5 cut(s) 398, 588, 765, 969, 1019
MvnI CGCG 3 cut(s) 478, 806, 1032
MwoI GCNNNNNNNGC 5 cut(s) 142, 548, 748, 824, 833
NdeI CATATG 1 cut(s) 1141
NdeII GATC 2 cut(s) 675, 960
NlaIII CATG 4 cut(s) 304, 511, 746, 906
NlaIV GGNNCC 4 cut(s) 29, 174, 579, 1084
NmuCI GTSAC 2 cut(s) 1098, 1167
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 1 cut(s) 511
PaeI GCATGC 1 cut(s) 511
PagI TCATGA 1 cut(s) 300
PauI GCGCGC 1 cut(s) 1030
PfeI GAWTC 1 cut(s) 1014
PinAI ACCGGT 1 cut(s) 169
PkrI GCNGC 5 cut(s) 147, 345, 805, 829, 1112
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Psp1406I AACGTT 1 cut(s) 571
Psp6I CCWGG 5 cut(s) 396, 586, 763, 967, 1017
PspFI CCCAGC 1 cut(s) 1249
PspGI CCWGG 5 cut(s) 396, 586, 763, 967, 1017
PspN4I GGNNCC 4 cut(s) 29, 174, 579, 1084
PteI GCGCGC 1 cut(s) 1030
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 3 cut(s) 772, 860, 1078
RsaNI GTAC 3 cut(s) 771, 859, 1077
SacII CCGCGG 1 cut(s) 807
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 5 cut(s) 146, 344, 804, 828, 1111
Sau3AI GATC 2 cut(s) 675, 960
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 5 cut(s) 398, 588, 765, 969, 1019
SduI GDGCHC 1 cut(s) 313
SfaNI GCATC 2 cut(s) 482, 760
SfcI CTRYAG 2 cut(s) 424, 789
Sfr303I CCGCGG 1 cut(s) 807
SgrBI CCGCGG 1 cut(s) 807
SmlI CTYRAG 2 cut(s) 131, 312
SmoI CTYRAG 2 cut(s) 131, 312
SphI GCATGC 1 cut(s) 511
Sse9I AATT 8 cut(s) 40, 338, 388, 530, 697, 746, 796, 1049
SspI AATATT 1 cut(s) 884
StyD4I CCNGG 5 cut(s) 396, 586, 763, 967, 1017
StyI CCWWGG 2 cut(s) 22, 1009
TaaI ACNGT 4 cut(s) 523, 556, 822, 1237
TaiI ACGT 1 cut(s) 574
TaqI TCGA 3 cut(s) 457, 738, 979
TasI AATT 8 cut(s) 40, 338, 388, 530, 697, 746, 796, 1049
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 1 cut(s) 1014
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TscAI CASTG 3 cut(s) 229, 559, 827
TseFI GTSAC 2 cut(s) 1098, 1167
TseI GCWGC 4 cut(s) 145, 343, 827, 1110
Tsp45I GTSAC 2 cut(s) 1098, 1167
TspDTI ATGAA 5 cut(s) 317, 431, 759, 1062, 1080
TspRI CASTG 3 cut(s) 229, 559, 827
XceI RCATGY 1 cut(s) 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 1201
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.