FvH4_2g32420

branched-chain-amino-acid aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004977
Physical Location & Seq
Forward (+)
24582305 .. 24585408
3104 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32420.t1

Sequence Viewer

Length: 1194 bp
ATGAACATGGTCTCCACTCCCGGCCTCGCAACTGCTTTTCCGACCCCGATCGCAACTACTACTCTCCGCACCTCCAAAGCTACTCTTCACCCCCGTCACTCGTGTTTCAAGTCTTTGATCACCACAAGCCTCAGAGCCCTTCAGTCTCATGCCCCCGGCTTTAGCCAAGTGTCTGAACTGCCTGATATAACTTGGGACAACGTTGGCTTCAACAATCCTATGACTACGGATTATATGTTTACTATGAAGTGTTCTGAAGATGGATACTTTTGTGATGGTGGGTTACAGCGTTTTGGTAAAATTGAATTGAACCCAGCTGCCTGTGTTTTGAACTATGGCCAGGGAGTAATTGAAGAATTGAAAGCATACAAGAAGCTAGATAATTCTATAGTACTCTTTCGTCCAGAGGAAAATGGATTGAGGATGAGAATGGGAGCAGAGCGCATGTGTATGCCTGCACCAACTGTCGAGCAGTTTATGGAAGCTGTGAAACTTACTGTGTTGGCAAATAGACGCTGGGTTCCCCCACCAAATAAAGGCTTTCTACATATCAAACCCGTACTGATGGGGAATGGACCTGTTCTTAGTGTTAAACCAGCTCCGGAATTCACCTTTCTCATATATGCTACTTCAATGGGAAACAACTTTAAGGAAGGACTGCAACCAATCAACTTAGTGGTTGAAAATAAAATCCATCGTGCAGTTCCTGGTGGAGTTGGAAGTGTTAATGCCATAGGGAATTATGCTCCGGTTTTGAAGGCACAGGTGGAAGCAAAAGCAAATGGTTTCTGTGATGTTTTGTACCTTGATTCTGTTCACAAAAAATATGTGGAACAGACTTCCACTGCTAACATCTTTCTAGTGAAGGACAAAATTATCTCCACTCCAGCACTGGAAGGGACAGTTTTGCCTGGTGTTACACGCAAAAGTATAATTGAAATTGCTCAAAGCCAAGGGTACCAGGTTGAGGAGCGCCCTGTGTCGATTGAGGAGTTGCTGGAAGCTGATGAAGTGTTCTGTACTAGAAATGCTGTCTGTTTATCACCTATAGGCAGCATAACTTACATGGGAAAAAGGGTGTCTTACCAAGAAACTGGACTTGGTGGAGTTTCTGAGCAACTGTATCAGGCTCTTTCAAATATACAGATGGGTCTTGCAGAAGACAATAGGGGTTGGACAGTAACCTTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

398

Amino Acids

43.58

Weight (kDa)

7.55

Isoelectric Point (pI)

51.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 116 - 353 1.1e-28 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 957
AccB1I GGYRCC 1 cut(s) 957
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 1 cut(s) 67
AclI AACGTT 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 605
AcuI CTGAAG 2 cut(s) 125, 276
AfaI GTAC 5 cut(s) 393, 561, 803, 959, 1021
AfiI CCNNNNNNNGG 2 cut(s) 536, 967
AjnI CCWGG 4 cut(s) 339, 706, 910, 960
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 6 cut(s) 80, 317, 376, 485, 599, 1004
AluI AGCT 6 cut(s) 80, 317, 376, 485, 599, 1004
Alw26I GTCTC 2 cut(s) 16, 150
AlwNI CAGNNNCTG 1 cut(s) 707
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 2 cut(s) 22, 337
ApeKI GCWGC 2 cut(s) 317, 1053
ApoI RAATTY 1 cut(s) 605
Asp718I GGTACC 1 cut(s) 957
AspLEI GCGC 2 cut(s) 444, 975
AspS9I GGNCC 1 cut(s) 575
AsuC2I CCSGG 2 cut(s) 21, 156
AsuHPI GGTGA 4 cut(s) 80, 112, 601, 1035
AvaII GGWCC 1 cut(s) 575
BaeI ACNNNNGTAYC 2 cut(s) 256, 289
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 1 cut(s) 957
BanII GRGCYC 1 cut(s) 139
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 1167
BbvI GCAGC 2 cut(s) 304, 1065
BccI CCATC 5 cut(s) 254, 269, 559, 702, 1141
BciT130I CCWGG 4 cut(s) 341, 708, 912, 962
BciVI GTATCC 1 cut(s) 257
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 21, 156
BcoDI GTCTC 2 cut(s) 16, 150
BfaI CTAG 3 cut(s) 377, 860, 1023
BfmI CTRYAG 2 cut(s) 387, 1047
BfoI RGCGCY 1 cut(s) 976
BfuI GTATCC 1 cut(s) 257
BisI GCNGC 2 cut(s) 318, 1054
BlsI GCNGC 2 cut(s) 319, 1055
BmcAI AGTACT 1 cut(s) 393
Bme1390I CCNGG 6 cut(s) 21, 156, 341, 708, 912, 962
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 2 cut(s) 522, 959
BmrFI CCNGG 6 cut(s) 21, 156, 341, 708, 912, 962
BpiI GAAGAC 1 cut(s) 1167
BpmI CTGGAG 1 cut(s) 870
BpuMI CCSGG 2 cut(s) 21, 156
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 3 cut(s) 154, 340, 952
BsaWI WCCGGW 2 cut(s) 601, 748
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 967
Bse1I ACTGG 2 cut(s) 897, 1099
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 4 cut(s) 341, 708, 912, 962
BseDI CCNNGG 3 cut(s) 154, 340, 952
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 2 cut(s) 536, 967
BseMII CTCAG 2 cut(s) 145, 1104
BseNI ACTGG 2 cut(s) 897, 1099
BseRI GAGGAG 2 cut(s) 983, 1004
BseXI GCAGC 2 cut(s) 304, 1065
BseYI CCCAGC 2 cut(s) 313, 516
BsgI GTGCAG 2 cut(s) 441, 720
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 2 cut(s) 24, 339
BshNI GGYRCC 1 cut(s) 957
BsiEI CGRYCG 1 cut(s) 51
BsiSI CCGG 4 cut(s) 21, 156, 602, 749
BslFI GGGAC 2 cut(s) 209, 913
BslI CCNNNNNNNGG 2 cut(s) 536, 967
BsmAI GTCTC 2 cut(s) 16, 150
BsmFI GGGAC 2 cut(s) 209, 913
BsnI GGCC 2 cut(s) 24, 339
Bso31I GGTCTC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 139
Bsp13I TCCGGA 1 cut(s) 601
Bsp143I GATC 2 cut(s) 48, 117
BspACI CCGC 1 cut(s) 67
BspANI GGCC 2 cut(s) 24, 339
BspCNI CTCAG 2 cut(s) 144, 1105
BspEI TCCGGA 1 cut(s) 601
BspLI GGNNCC 2 cut(s) 522, 959
BspT107I GGYRCC 1 cut(s) 957
BspTNI GGTCTC 1 cut(s) 16
BsrI ACTGG 2 cut(s) 897, 1099
BssECI CCNNGG 3 cut(s) 154, 340, 952
BssMI GATC 2 cut(s) 48, 117
BssSI CACGAG 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 952
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 4 cut(s) 341, 708, 912, 962
Bst4CI ACNGT 5 cut(s) 466, 499, 904, 1122, 1180
Bst6I CTCTTC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 4 cut(s) 131, 584, 673, 1113
BstF5I GGATG 1 cut(s) 429
BstH2I RGCGCY 1 cut(s) 976
BstHHI GCGC 2 cut(s) 444, 975
BstKTI GATC 2 cut(s) 51, 120
BstMAI GTCTC 2 cut(s) 16, 150
BstMBI GATC 2 cut(s) 48, 117
BstMCI CGRYCG 1 cut(s) 51
BstNI CCWGG 4 cut(s) 341, 708, 912, 962
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 6 cut(s) 19, 154, 339, 706, 910, 960
BstSFI CTRYAG 2 cut(s) 387, 1047
BstV1I GCAGC 2 cut(s) 304, 1065
BstV2I GAAGAC 1 cut(s) 1167
BstXI CCANNNNNNTGG 1 cut(s) 1094
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 24, 339
BtsCI GGATG 1 cut(s) 429
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 2 cut(s) 843, 890
Cac8I GCNNGC 1 cut(s) 456
CaiI CAGNNNCTG 1 cut(s) 707
CfoI GCGC 2 cut(s) 444, 975
Cfr13I GGNCC 1 cut(s) 575
CseI GACGC 1 cut(s) 522
CsiI ACCWGGT 1 cut(s) 960
Csp6I GTAC 5 cut(s) 392, 560, 802, 958, 1020
CviAII CATG 4 cut(s) 7, 149, 445, 1066
CviQI GTAC 5 cut(s) 392, 560, 802, 958, 1020
DdeI CTNAG 4 cut(s) 131, 584, 673, 1113
DpnI GATC 2 cut(s) 50, 119
DpnII GATC 2 cut(s) 48, 117
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 2 cut(s) 125, 276
EcoRI GAATTC 1 cut(s) 605
EcoRII CCWGG 4 cut(s) 339, 706, 910, 960
EcoT14I CCWWGG 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 952
FaeI CATG 4 cut(s) 10, 152, 448, 1069
FalI AAGNNNNNCTT 4 cut(s) 69, 101, 1066, 1098
FaqI GGGAC 2 cut(s) 209, 913
FatI CATG 4 cut(s) 6, 148, 444, 1065
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 318, 1054
FokI GGATG 1 cut(s) 436
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 2 cut(s) 318, 1054
FspBI CTAG 3 cut(s) 377, 860, 1023
GlaI GCGC 2 cut(s) 443, 974
GluI GCNGC 2 cut(s) 318, 1054
GsaI CCCAGC 2 cut(s) 317, 520
GsuI CTGGAG 1 cut(s) 870
HaeII RGCGCY 1 cut(s) 976
HaeIII GGCC 2 cut(s) 24, 339
HapII CCGG 4 cut(s) 21, 156, 602, 749
HgaI GACGC 1 cut(s) 522
HhaI GCGC 2 cut(s) 444, 975
Hin1II CATG 4 cut(s) 10, 152, 448, 1069
Hin6I GCGC 2 cut(s) 442, 973
HinP1I GCGC 2 cut(s) 442, 973
HinfI GANTC 1 cut(s) 809
HpaII CCGG 4 cut(s) 21, 156, 602, 749
HphI GGTGA 4 cut(s) 80, 112, 601, 1035
Hpy166II GTNNAC 2 cut(s) 240, 817
Hpy188I TCNGA 5 cut(s) 42, 134, 175, 256, 1114
Hpy188III TCNNGA 2 cut(s) 404, 602
Hpy8I GTNNAC 2 cut(s) 240, 817
HpyAV CCTTC 5 cut(s) 149, 647, 751, 859, 890
HpyCH4III ACNGT 5 cut(s) 466, 499, 904, 1122, 1180
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 4 cut(s) 458, 661, 701, 1157
HpyF3I CTNAG 4 cut(s) 131, 584, 673, 1113
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 4 cut(s) 10, 152, 448, 1069
HspAI GCGC 2 cut(s) 442, 973
Kpn2I TCCGGA 1 cut(s) 601
KpnI GGTACC 1 cut(s) 961
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 2 cut(s) 48, 117
LmnI GCTCC 4 cut(s) 434, 604, 751, 970
Lsp1109I GCAGC 2 cut(s) 304, 1065
MabI ACCWGGT 1 cut(s) 960
MaeI CTAG 3 cut(s) 377, 860, 1023
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 4 cut(s) 95, 282, 916, 1180
MalI GATC 2 cut(s) 50, 119
MboI GATC 2 cut(s) 48, 117
MboII GAAGA 4 cut(s) 77, 269, 365, 1172
MhlI GDGCHC 1 cut(s) 139
MlsI TGGCCA 1 cut(s) 339
MluNI TGGCCA 1 cut(s) 339
MmeI TCCRAC 3 cut(s) 65, 697, 1154
MnlI CCTC 7 cut(s) 35, 82, 140, 400, 414, 961, 982
Mox20I TGGCCA 1 cut(s) 339
MroI TCCGGA 1 cut(s) 601
MscI TGGCCA 1 cut(s) 339
MseI TTAA 3 cut(s) 591, 648, 726
MslI CAYNNNNRTG 1 cut(s) 449
Msp20I TGGCCA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 4 cut(s) 21, 156, 602, 749
MspR9I CCNGG 6 cut(s) 21, 156, 341, 708, 912, 962
MvaI CCWGG 4 cut(s) 341, 708, 912, 962
NciI CCSGG 2 cut(s) 21, 156
NdeII GATC 2 cut(s) 48, 117
NlaIII CATG 4 cut(s) 10, 152, 448, 1069
NlaIV GGNNCC 2 cut(s) 522, 959
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 448
PfeI GAWTC 1 cut(s) 809
PkrI GCNGC 2 cut(s) 319, 1055
Ple19I CGATCG 1 cut(s) 51
Psp1406I AACGTT 1 cut(s) 201
Psp6I CCWGG 4 cut(s) 339, 706, 910, 960
PspFI CCCAGC 2 cut(s) 313, 516
PspGI CCWGG 4 cut(s) 339, 706, 910, 960
PspN4I GGNNCC 2 cut(s) 522, 959
PspPI GGNCC 1 cut(s) 575
PstNI CAGNNNCTG 1 cut(s) 707
PvuI CGATCG 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 5 cut(s) 393, 561, 803, 959, 1021
RsaNI GTAC 5 cut(s) 392, 560, 802, 958, 1020
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 3 cut(s) 591, 648, 726
SatI GCNGC 2 cut(s) 318, 1054
Sau3AI GATC 2 cut(s) 48, 117
Sau96I GGNCC 1 cut(s) 575
ScaI AGTACT 1 cut(s) 393
ScrFI CCNGG 6 cut(s) 21, 156, 341, 708, 912, 962
SduI GDGCHC 1 cut(s) 139
SexAI ACCWGGT 1 cut(s) 960
SfcI CTRYAG 2 cut(s) 387, 1047
SinI GGWCC 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 449
SsiI CCGC 1 cut(s) 67
SspMI CTAG 3 cut(s) 377, 860, 1023
StyD4I CCNGG 6 cut(s) 19, 154, 339, 706, 910, 960
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 5 cut(s) 466, 499, 904, 1122, 1180
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 468, 983
TatI WGTACW 2 cut(s) 391, 1019
TfiI GAWTC 1 cut(s) 809
Tru1I TTAA 3 cut(s) 591, 648, 726
Tru9I TTAA 3 cut(s) 591, 648, 726
TscAI CASTG 2 cut(s) 850, 897
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 2 cut(s) 317, 1053
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 17, 260, 1023
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 2 cut(s) 850, 897
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 1 cut(s) 605
XceI RCATGY 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 889
XspI CTAG 3 cut(s) 377, 860, 1023
ZrmI AGTACT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.