MD15G1063000.v1.1

branched-chain-amino-acid aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004977
Physical Location & Seq
Forward (+)
4337988 .. 4340771
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1063000.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGCTTTTGATGATGCAGCAGCAGCTTCTACTCGCGCCTTCTTCGCTTCGGCCAGCGGCTCCGATCACCACCTCCGCCACAAGCCTCAGAGCCGTCACTCCATCTGGTGTCGCTCATGATCAAACTTCTGAATTGCCTGACACAACATGGGACACGCTCGGATTCAACAAACCCATGTCGACAGATTACATGTTTGTTATGAAAAGTTCCAAAGATGCAAACTTTTTGGATGGTGGGTTACAACGTTTCGAAAAAATTGAGTTCAACCCAGCTGCCTGTGGAATAATTGAAGAATTGAAAGCATACAAGAAACATGACGGTTCTATATTACTATTTCACCTAGAGGAACATGGGTTGCGGATGAGGACGGGCGCAGAGCGCCTGTGTATGCCTGCACCAACTGTTGAGCAGTTTGTGGAAGCTGTAAAAGCTACTGTCTTGGCAAATCGACGTTGGCATGTTCTAATTATGGTGCTCAGTGCATCATTACAGGTTCCCCCACGGAGTCAAGGCTTTCTTCATATTCGACCACTACTTATTGGGAATGGACCTGTTCTTAGTCTTACACCAGCTCCAGAATTCACCTTTCTGATTTATGTTTCTCCAATGGGGAACTATTTTGAGGGGGGCTTGCAACCAATCAATTTGGCGGTAGAAAATGAAATCCATCGCGCAGTCCGTGGTGGAGCTGGAAGTGTGAAAGCCATAAGAAATTATGGTGCTATTTTGAAGGCACAGGTGGACGCTAAAGCAAATGGTTTCTCTGATGTTTTGTACCTTGATTCTGTTCACAACAGATACATTGAAGAGACTTCGACTGCGAACATTTTTCTAGTGAAGGATAATGTAATCTCCACTCCGGCACGGCACGAAGGGACCATTTTGCCTGGCATTACCCGTAACTCTGTAATTGAAATTGCTCGCATCGAAGGGTACCAGGTTGAGGAACGCCTTGTGTCGTTTGAGGAATTGTTTGAAGCCGATGAAGTATTTTGTACTGGAAATGCTGTCTTAACACCGGTGCGCAGCATAACTTACCTCGATAAAAGGGTGTCTTACCTTGAAACTGGCCCCGGTGGTGTCGTGTCTCAGGAACTGCACCGGGCGCTTTCAAATATACAGATGGGTCTTACAGAATTCGGCGGGACAGTAACCTTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

388

Amino Acids

42.56

Weight (kDa)

5.96

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 95 - 344 2e-29 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1025
Acc65I GGTACC 1 cut(s) 933
AccB1I GGYRCC 1 cut(s) 933
AccI GTMKAC 1 cut(s) 179
AccII CGCG 2 cut(s) 35, 672
AciI CCGC 5 cut(s) 56, 75, 358, 650, 1143
AclI AACGTT 1 cut(s) 244
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 2 cut(s) 578, 1136
AfaI GTAC 3 cut(s) 776, 935, 997
AfiI CCNNNNNNNGG 1 cut(s) 943
AflIII ACRYGT 1 cut(s) 189
AgeI ACCGGT 1 cut(s) 1018
AjnI CCWGG 2 cut(s) 886, 936
AluBI AGCT 6 cut(s) 25, 272, 422, 431, 572, 689
AluI AGCT 6 cut(s) 25, 272, 422, 431, 572, 689
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 2 cut(s) 803, 1092
AlwNI CAGNNNCTG 1 cut(s) 1096
AoxI GGCC 2 cut(s) 50, 1069
ApeKI GCWGC 5 cut(s) 16, 19, 22, 272, 1026
ApoI RAATTY 2 cut(s) 578, 1136
AsiGI ACCGGT 1 cut(s) 1018
Asp718I GGTACC 1 cut(s) 933
AspLEI GCGC 6 cut(s) 37, 374, 381, 674, 1026, 1108
AspS9I GGNCC 3 cut(s) 548, 876, 1070
AsuC2I CCSGG 2 cut(s) 1074, 1103
AsuHPI GGTGA 3 cut(s) 58, 329, 574
AsuII TTCGAA 1 cut(s) 249
AvaII GGWCC 2 cut(s) 548, 876
BanI GGYRCC 1 cut(s) 933
Bbv12I GWGCWC 1 cut(s) 477
BbvI GCAGC 5 cut(s) 28, 31, 34, 259, 1038
BccI CCATC 4 cut(s) 109, 224, 675, 1117
BceAI ACGGC 2 cut(s) 77, 881
BciT130I CCWGG 2 cut(s) 888, 938
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 2 cut(s) 1074, 1103
BcoDI GTCTC 2 cut(s) 803, 1092
BfaI CTAG 2 cut(s) 341, 833
BfoI RGCGCY 2 cut(s) 382, 1109
BisI GCNGC 6 cut(s) 17, 20, 23, 57, 273, 1027
BlsI GCNGC 6 cut(s) 18, 21, 24, 58, 274, 1028
Bme1390I CCNGG 4 cut(s) 888, 938, 1074, 1103
Bme18I GGWCC 2 cut(s) 548, 876
BmgT120I GGNCC 3 cut(s) 548, 876, 1070
BmiI GGNNCC 5 cut(s) 60, 495, 877, 935, 1072
BmrFI CCNGG 4 cut(s) 888, 938, 1074, 1103
BmsI GCATC 4 cut(s) 3, 205, 491, 933
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 2 cut(s) 1074, 1103
BsaJI CCNNGG 3 cut(s) 500, 679, 1072
BsaWI WCCGGW 1 cut(s) 1018
BsaXI ACNNNNNCTCC 2 cut(s) 556, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 943
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 2 cut(s) 1003, 1072
BseBI CCWGG 2 cut(s) 888, 938
BseDI CCNNGG 3 cut(s) 500, 679, 1072
BseGI GGATG 2 cut(s) 235, 366
BseLI CCNNNNNNNGG 1 cut(s) 943
BseMII CTCAG 3 cut(s) 100, 490, 1103
BseNI ACTGG 2 cut(s) 1003, 1072
BseXI GCAGC 5 cut(s) 28, 31, 34, 259, 1038
BseYI CCCAGC 1 cut(s) 268
BsgI GTGCAG 2 cut(s) 378, 1082
Bsh1236I CGCG 2 cut(s) 35, 672
BshFI GGCC 2 cut(s) 52, 1071
BshNI GGYRCC 1 cut(s) 933
BshTI ACCGGT 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 477
BsiSI CCGG 4 cut(s) 860, 1019, 1074, 1102
BslFI GGGAC 3 cut(s) 164, 889, 1159
BslI CCNNNNNNNGG 1 cut(s) 943
BsmAI GTCTC 2 cut(s) 803, 1092
BsmFI GGGAC 3 cut(s) 164, 889, 1159
BsnI GGCC 2 cut(s) 52, 1071
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 477
Bsp143I GATC 2 cut(s) 63, 118
BspACI CCGC 5 cut(s) 56, 75, 358, 650, 1143
BspANI GGCC 2 cut(s) 52, 1071
BspCNI CTCAG 3 cut(s) 99, 489, 1102
BspFNI CGCG 2 cut(s) 35, 672
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 5 cut(s) 60, 495, 877, 935, 1072
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 933
BsrFI RCCGGY 1 cut(s) 1018
BsrI ACTGG 2 cut(s) 1003, 1072
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 3 cut(s) 500, 679, 1072
BssMI GATC 2 cut(s) 63, 118
Bst2UI CCWGG 2 cut(s) 888, 938
Bst4CI ACNGT 4 cut(s) 320, 403, 436, 1150
Bst6I CTCTTC 1 cut(s) 801
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 4 cut(s) 54, 393, 632, 922
BstDEI CTNAG 4 cut(s) 86, 476, 557, 1089
BstDSI CCRYGG 2 cut(s) 500, 679
BstF5I GGATG 2 cut(s) 235, 366
BstFNI CGCG 2 cut(s) 35, 672
BstH2I RGCGCY 2 cut(s) 382, 1109
BstHHI GCGC 6 cut(s) 37, 374, 381, 674, 1026, 1108
BstKTI GATC 2 cut(s) 66, 121
BstMAI GTCTC 2 cut(s) 803, 1092
BstMBI GATC 2 cut(s) 63, 118
BstMWI GCNNNNNNNGC 5 cut(s) 22, 43, 378, 428, 1105
BstNI CCWGG 2 cut(s) 888, 938
BstNSI RCATGY 2 cut(s) 193, 461
BstSCI CCNGG 4 cut(s) 886, 936, 1072, 1101
BstUI CGCG 2 cut(s) 35, 672
BstV1I GCAGC 5 cut(s) 28, 31, 34, 259, 1038
BsuRI GGCC 2 cut(s) 52, 1071
BtgI CCRYGG 2 cut(s) 500, 679
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 2 cut(s) 235, 366
BtsIMutI CAGTG 1 cut(s) 484
Cac8I GCNNGC 4 cut(s) 54, 393, 632, 922
CaiI CAGNNNCTG 1 cut(s) 1096
CciI TCATGA 1 cut(s) 115
CfoI GCGC 6 cut(s) 37, 374, 381, 674, 1026, 1108
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 3 cut(s) 548, 876, 1070
CseI GACGC 1 cut(s) 752
CsiI ACCWGGT 1 cut(s) 936
Csp6I GTAC 3 cut(s) 775, 934, 996
CspAI ACCGGT 1 cut(s) 1018
CviAII CATG 7 cut(s) 116, 147, 175, 190, 314, 350, 458
CviQI GTAC 3 cut(s) 775, 934, 996
DdeI CTNAG 4 cut(s) 86, 476, 557, 1089
DpnI GATC 2 cut(s) 65, 120
DpnII GATC 2 cut(s) 63, 118
EaeI YGGCCR 1 cut(s) 50
Eam1104I CTCTTC 1 cut(s) 801
EarI CTCTTC 1 cut(s) 801
EciI GGCGGA 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 548, 876
EcoRI GAATTC 2 cut(s) 578, 1136
EcoRII CCWGG 2 cut(s) 886, 936
FaeI CATG 7 cut(s) 119, 150, 178, 193, 317, 353, 461
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 1039, 1071
FaqI GGGAC 3 cut(s) 164, 889, 1159
FatI CATG 7 cut(s) 115, 146, 174, 189, 313, 349, 457
FauI CCCGC 1 cut(s) 1136
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 6 cut(s) 17, 20, 23, 57, 273, 1027
FokI GGATG 2 cut(s) 242, 373
Fsp4HI GCNGC 6 cut(s) 17, 20, 23, 57, 273, 1027
FspBI CTAG 2 cut(s) 341, 833
FspI TGCGCA 1 cut(s) 1025
GlaI GCGC 6 cut(s) 36, 373, 380, 673, 1025, 1107
GluI GCNGC 6 cut(s) 17, 20, 23, 57, 273, 1027
GsaI CCCAGC 1 cut(s) 272
GsuI CTGGAG 1 cut(s) 558
HaeII RGCGCY 2 cut(s) 382, 1109
HaeIII GGCC 2 cut(s) 52, 1071
HapII CCGG 4 cut(s) 860, 1019, 1074, 1102
HgaI GACGC 1 cut(s) 752
HhaI GCGC 6 cut(s) 37, 374, 381, 674, 1026, 1108
Hin1II CATG 7 cut(s) 119, 150, 178, 193, 317, 353, 461
Hin6I GCGC 6 cut(s) 35, 372, 379, 672, 1024, 1106
HinP1I GCGC 6 cut(s) 35, 372, 379, 672, 1024, 1106
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 3 cut(s) 162, 505, 782
HpaII CCGG 4 cut(s) 860, 1019, 1074, 1102
HphI GGTGA 3 cut(s) 58, 329, 574
Hpy166II GTNNAC 3 cut(s) 180, 742, 790
Hpy188I TCNGA 6 cut(s) 63, 89, 130, 161, 591, 766
Hpy188III TCNNGA 3 cut(s) 116, 575, 1091
Hpy8I GTNNAC 3 cut(s) 180, 742, 790
Hpy99I CGWCG 1 cut(s) 453
HpyAV CCTTC 5 cut(s) 48, 724, 832, 866, 923
HpyCH4III ACNGT 4 cut(s) 320, 403, 436, 1150
HpyCH4IV ACGT 2 cut(s) 244, 451
HpyCH4V TGCA 6 cut(s) 16, 218, 395, 482, 634, 1099
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 43, 378, 428, 1105
HpyF3I CTNAG 4 cut(s) 86, 476, 557, 1089
HpySE526I ACGT 2 cut(s) 244, 451
Hsp92II CATG 7 cut(s) 119, 150, 178, 193, 317, 353, 461
HspAI GCGC 6 cut(s) 35, 372, 379, 672, 1024, 1106
KpnI GGTACC 1 cut(s) 937
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 63, 118
LmnI GCTCC 3 cut(s) 64, 577, 686
Lsp1109I GCAGC 5 cut(s) 28, 31, 34, 259, 1038
LweI GCATC 4 cut(s) 3, 205, 491, 933
MabI ACCWGGT 1 cut(s) 936
MaeI CTAG 2 cut(s) 341, 833
MaeII ACGT 2 cut(s) 244, 451
MaeIII GTNAC 4 cut(s) 94, 237, 899, 1150
MalI GATC 2 cut(s) 65, 120
MboI GATC 2 cut(s) 63, 118
MboII GAAGA 4 cut(s) 33, 302, 509, 818
MhlI GDGCHC 1 cut(s) 477
MlyI GAGTC 1 cut(s) 514
MnlI CCTC 8 cut(s) 82, 95, 337, 357, 616, 937, 958, 1049
MseI TTAA 1 cut(s) 1013
MspA1I CMGCKG 2 cut(s) 56, 272
MspI CCGG 4 cut(s) 860, 1019, 1074, 1102
MspR9I CCNGG 4 cut(s) 888, 938, 1074, 1103
MvaI CCWGG 2 cut(s) 888, 938
MvnI CGCG 2 cut(s) 35, 672
MwoI GCNNNNNNNGC 5 cut(s) 22, 43, 378, 428, 1105
NciI CCSGG 2 cut(s) 1074, 1103
NdeII GATC 2 cut(s) 63, 118
NlaIII CATG 7 cut(s) 119, 150, 178, 193, 317, 353, 461
NlaIV GGNNCC 5 cut(s) 60, 495, 877, 935, 1072
NmuCI GTSAC 1 cut(s) 94
NsbI TGCGCA 1 cut(s) 1025
NspI RCATGY 2 cut(s) 193, 461
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 1 cut(s) 115
PciI ACATGT 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 676
PfeI GAWTC 2 cut(s) 162, 782
PinAI ACCGGT 1 cut(s) 1018
PkrI GCNGC 6 cut(s) 18, 21, 24, 58, 274, 1028
PleI GAGTC 1 cut(s) 513
PpsI GAGTC 1 cut(s) 513
PscI ACATGT 1 cut(s) 189
Psp1406I AACGTT 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 886, 936
PspFI CCCAGC 1 cut(s) 268
PspGI CCWGG 2 cut(s) 886, 936
PspN4I GGNNCC 5 cut(s) 60, 495, 877, 935, 1072
PspPI GGNCC 3 cut(s) 548, 876, 1070
PstNI CAGNNNCTG 1 cut(s) 1096
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 3 cut(s) 776, 935, 997
RsaNI GTAC 3 cut(s) 775, 934, 996
SalI GTCGAC 1 cut(s) 178
SaqAI TTAA 1 cut(s) 1013
SatI GCNGC 6 cut(s) 17, 20, 23, 57, 273, 1027
Sau3AI GATC 2 cut(s) 63, 118
Sau96I GGNCC 3 cut(s) 548, 876, 1070
SchI GAGTC 1 cut(s) 514
ScrFI CCNGG 4 cut(s) 888, 938, 1074, 1103
SduI GDGCHC 1 cut(s) 477
SexAI ACCWGGT 1 cut(s) 936
SfaNI GCATC 4 cut(s) 3, 205, 491, 933
SfuI TTCGAA 1 cut(s) 249
SgrAI CRCCGGYG 1 cut(s) 1018
SinI GGWCC 2 cut(s) 548, 876
SsiI CCGC 5 cut(s) 56, 75, 358, 650, 1143
SspMI CTAG 2 cut(s) 341, 833
StyD4I CCNGG 4 cut(s) 886, 936, 1072, 1101
TaaI ACNGT 4 cut(s) 320, 403, 436, 1150
TaiI ACGT 2 cut(s) 247, 454
TaqI TCGA 7 cut(s) 179, 249, 448, 526, 815, 927, 1041
TatI WGTACW 1 cut(s) 995
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 162, 782
Tru1I TTAA 1 cut(s) 1013
Tru9I TTAA 1 cut(s) 1013
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 5 cut(s) 16, 19, 22, 272, 1026
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 4 cut(s) 215, 509, 675, 999
TspGWI ACGGA 2 cut(s) 517, 668
TspRI CASTG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 548, 876
XapI RAATTY 2 cut(s) 578, 1136
XceI RCATGY 2 cut(s) 193, 461
XmiI GTMKAC 1 cut(s) 179
XspI CTAG 2 cut(s) 341, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.