Rh6DG493500

branched-chain-amino-acid aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004977
Physical Location & Seq
Reverse (-)
65256140 .. 65259329
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG493500.1

Sequence Viewer

Length: 1290 bp
ATGAGGAGACGTTCCTTTTGTCCCCGAACCGATCTCCGATCAACAAACCCAAACCCTCCGCACTCACACTCATCGGAGACACAGACACAGACGCAGATGGGTTCCACTTCCGCCCTCGCACTGGCTTTTCCGTCCCCAATCGCAACTACTCTCCGCACCTCCAAAAACCCCCACTCCTCGTCTTACAAGCTCCTCCGTCTCTGTTTGATCACCACAAGGCTCCAAGCCATTCAGTCTCATGCCTCTGGTGGCTATAGCCAAGCGTCTGAATTGCCTGATATAAGTTGGGACAATGTTGGCTTCGACAACCCTATGACTACCGATTACATGTTTACTGTGAAGTACTGTTCTGAAGATGGGAACTTTTCCGATGGTGGGTTACAGCGTTTTGGTAAAATTGAGTTGAACCCAGCTGCCTGTGTTTTGAACTATGGTCAGGGAGTAATTGAAGAATTGAAAGCGTACAAGAAGCTAGATAATTCTATAGTACTATTTCGTCCAGAGGAAAATGGATTGAGGATGAGAATGGGAGCAGAGCGCATGTGTATGCCTGCACCAACTGTTGAGCAGTTTGTGGAAGCTGTGAAACTTACTGTATTGGCAAATAGACGTTGGGTTCCGCCACCAAATAAAGGCTTTCTACATATCAAACCACTACTTATGGGGAATGGACCTGTTCTTAGTCTTACACCGGCTCCAGAATTCACCTTTCTCATATATGCTTCTTCGATGGGGAACACCTTTAAGGGGGGACTGGAACCAATCAACTTAGTGGTTGAAAATGAAATCCATCGTGCAGTTCGTGGTGGAGCTGGAACTGTTAATGCCATAGGGAATTATGCTATGGTTTTGAAGACACAGGTGGAGGCAAAAGCAGATGGTTTCTCTGATGTTTTGTACCTTGATTCTGTTCACAAAAGATATGTGGAACAGACTTCCACTGCTAACATCTTTCTAGTGAAGGACAAAATTATTTCCACTCCAGCACTGGAAGGGACAATTTTGCCTGGCGTTACACGCAAAAGTATAATTGAAATTGCTTGCAGCCAAGGGTACCAGGTTGAGGAGCGCCCTGTGTCGATTGAGGAATTGCTAGAAGCTGATGAAGTGTTCTGTACTGGATATGCTGTCTGTTTATCACCTATAGGCAGCATAACTTACCTGGGAAAAAGGGTGTCTTACCAAGAAACTGGACTTGGTGGAGTTTCTGAGCAACTGTATCAGGCTCTTTCAAATATACAGATGGGTCTTGCAGAAGAAAATAGGGGTTGGATAGTAACCTTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

429

Amino Acids

47.13

Weight (kDa)

6.68

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 148 - 385 2.3e-26 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1053
AccB1I GGYRCC 1 cut(s) 1053
AciI CCGC 4 cut(s) 59, 111, 154, 620
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 6 cut(s) 344, 464, 489, 899, 1055, 1117
AfiI CCNNNNNNNGG 5 cut(s) 121, 375, 632, 747, 1063
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 9 cut(s) 406, 427, 449, 457, 779, 853, 1034, 1233, 1285
AjnI CCWGG 3 cut(s) 1006, 1056, 1161
AjuI GAANNNNNNNTTGG 2 cut(s) 1179, 1211
AluBI AGCT 6 cut(s) 190, 413, 472, 581, 812, 1100
AluI AGCT 6 cut(s) 190, 413, 472, 581, 812, 1100
Alw26I GTCTC 3 cut(s) 71, 203, 240
ApeKI GCWGC 3 cut(s) 413, 1044, 1149
ApoI RAATTY 1 cut(s) 701
ArsI GACNNNNNNTTYG 1 cut(s) 32
Asp718I GGTACC 1 cut(s) 1053
AspLEI GCGC 2 cut(s) 540, 1071
AspS9I GGNCC 1 cut(s) 671
AsuHPI GGTGA 3 cut(s) 202, 697, 1131
AvaII GGWCC 1 cut(s) 671
BanI GGYRCC 1 cut(s) 1053
BbsI GAAGAC 1 cut(s) 860
BbvI GCAGC 3 cut(s) 400, 1056, 1161
BccI CCATC 7 cut(s) 91, 350, 365, 724, 798, 872, 1237
BciT130I CCWGG 3 cut(s) 1008, 1058, 1163
BclI TGATCA 1 cut(s) 207
BcoDI GTCTC 3 cut(s) 71, 203, 240
BfaI CTAG 3 cut(s) 473, 956, 1094
BfmI CTRYAG 3 cut(s) 253, 483, 1143
BfoI RGCGCY 1 cut(s) 1072
BisI GCNGC 3 cut(s) 414, 1045, 1150
BlsI GCNGC 3 cut(s) 415, 1046, 1151
BmcAI AGTACT 2 cut(s) 344, 489
Bme1390I CCNGG 3 cut(s) 1008, 1058, 1163
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 6 cut(s) 103, 221, 618, 696, 759, 1055
BmrFI CCNGG 3 cut(s) 1008, 1058, 1163
BpiI GAAGAC 1 cut(s) 860
BpmI CTGGAG 2 cut(s) 681, 966
BsaJI CCNNGG 2 cut(s) 1048, 1162
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 679, 709
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 375, 632, 747, 1063
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 5 cut(s) 126, 759, 993, 1123, 1195
BseBI CCWGG 3 cut(s) 1008, 1058, 1163
BseDI CCNNGG 2 cut(s) 1048, 1162
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 5 cut(s) 121, 375, 632, 747, 1063
BseMII CTCAG 1 cut(s) 1200
BseNI ACTGG 5 cut(s) 126, 759, 993, 1123, 1195
BseRI GAGGAG 4 cut(s) 19, 166, 182, 1079
BseXI GCAGC 3 cut(s) 400, 1056, 1161
BseYI CCCAGC 1 cut(s) 409
BsgI GTGCAG 2 cut(s) 537, 816
BshNI GGYRCC 1 cut(s) 1053
BsiSI CCGG 1 cut(s) 692
BslFI GGGAC 5 cut(s) 6, 118, 302, 765, 1009
BslI CCNNNNNNNGG 5 cut(s) 121, 375, 632, 747, 1063
BsmAI GTCTC 3 cut(s) 71, 203, 240
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 5 cut(s) 6, 118, 302, 765, 1009
Bsp143I GATC 3 cut(s) 31, 38, 207
BspACI CCGC 4 cut(s) 59, 111, 154, 620
BspCNI CTCAG 1 cut(s) 1201
BspLI GGNNCC 6 cut(s) 103, 221, 618, 696, 759, 1055
BspT107I GGYRCC 1 cut(s) 1053
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 5 cut(s) 126, 759, 993, 1123, 1195
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 2 cut(s) 1048, 1162
BssMI GATC 3 cut(s) 31, 38, 207
BssT1I CCWWGG 1 cut(s) 1048
Bst2UI CCWGG 3 cut(s) 1008, 1058, 1163
Bst4CI ACNGT 6 cut(s) 337, 347, 562, 595, 820, 1218
BstC8I GCNNGC 2 cut(s) 552, 1042
BstDEI CTNAG 3 cut(s) 680, 769, 1209
BstF5I GGATG 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 1072
BstHHI GCGC 2 cut(s) 540, 1071
BstKTI GATC 3 cut(s) 34, 41, 210
BstMAI GTCTC 3 cut(s) 71, 203, 240
BstMBI GATC 3 cut(s) 31, 38, 207
BstMWI GCNNNNNNNGC 1 cut(s) 1017
BstNI CCWGG 3 cut(s) 1008, 1058, 1163
BstNSI RCATGY 2 cut(s) 331, 544
BstSCI CCNGG 3 cut(s) 1006, 1056, 1161
BstSFI CTRYAG 3 cut(s) 253, 483, 1143
BstV1I GCAGC 3 cut(s) 400, 1056, 1161
BstV2I GAAGAC 1 cut(s) 860
BstXI CCANNNNNNTGG 1 cut(s) 1190
BtsCI GGATG 1 cut(s) 525
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 3 cut(s) 119, 939, 986
Cac8I GCNNGC 2 cut(s) 552, 1042
CfoI GCGC 2 cut(s) 540, 1071
Cfr10I RCCGGY 1 cut(s) 691
Cfr13I GGNCC 1 cut(s) 671
CseI GACGC 2 cut(s) 100, 252
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 6 cut(s) 343, 463, 488, 898, 1054, 1116
CviAII CATG 3 cut(s) 239, 328, 541
CviQI GTAC 6 cut(s) 343, 463, 488, 898, 1054, 1116
DdeI CTNAG 3 cut(s) 680, 769, 1209
DpnI GATC 3 cut(s) 33, 40, 209
DpnII GATC 3 cut(s) 31, 38, 207
EciI GGCGGA 2 cut(s) 100, 609
Eco130I CCWWGG 1 cut(s) 1048
Eco47I GGWCC 1 cut(s) 671
Eco57I CTGAAG 1 cut(s) 372
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 3 cut(s) 1006, 1056, 1161
EcoT14I CCWWGG 1 cut(s) 1048
ErhI CCWWGG 1 cut(s) 1048
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 3 cut(s) 242, 331, 544
FalI AAGNNNNNCTT 2 cut(s) 1162, 1194
FaqI GGGAC 5 cut(s) 6, 118, 302, 765, 1009
FatI CATG 3 cut(s) 238, 327, 540
FbaI TGATCA 1 cut(s) 207
Fnu4HI GCNGC 3 cut(s) 414, 1045, 1150
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 3 cut(s) 414, 1045, 1150
FspBI CTAG 3 cut(s) 473, 956, 1094
GlaI GCGC 2 cut(s) 539, 1070
GluI GCNGC 3 cut(s) 414, 1045, 1150
GsaI CCCAGC 1 cut(s) 413
GsuI CTGGAG 2 cut(s) 681, 966
HaeII RGCGCY 1 cut(s) 1072
HapII CCGG 1 cut(s) 692
HgaI GACGC 2 cut(s) 100, 252
HhaI GCGC 2 cut(s) 540, 1071
Hin1II CATG 3 cut(s) 242, 331, 544
Hin6I GCGC 2 cut(s) 538, 1069
HinP1I GCGC 2 cut(s) 538, 1069
HinfI GANTC 1 cut(s) 905
HpaII CCGG 1 cut(s) 692
HphI GGTGA 3 cut(s) 202, 697, 1131
Hpy166II GTNNAC 2 cut(s) 333, 913
Hpy188I TCNGA 7 cut(s) 38, 76, 268, 352, 370, 889, 1210
Hpy188III TCNNGA 2 cut(s) 500, 698
Hpy8I GTNNAC 2 cut(s) 333, 913
HpyAV CCTTC 2 cut(s) 955, 986
HpyCH4III ACNGT 6 cut(s) 337, 347, 562, 595, 820, 1218
HpyCH4IV ACGT 2 cut(s) 10, 610
HpyCH4V TGCA 4 cut(s) 554, 797, 1044, 1253
HpyF10VI GCNNNNNNNGC 1 cut(s) 1017
HpyF3I CTNAG 3 cut(s) 680, 769, 1209
HpySE526I ACGT 2 cut(s) 10, 610
Hsp92II CATG 3 cut(s) 242, 331, 544
HspAI GCGC 2 cut(s) 538, 1069
KpnI GGTACC 1 cut(s) 1057
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 3 cut(s) 31, 38, 207
LmnI GCTCC 6 cut(s) 195, 225, 530, 700, 809, 1066
Lsp1109I GCAGC 3 cut(s) 400, 1056, 1161
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 3 cut(s) 473, 956, 1094
MaeII ACGT 2 cut(s) 10, 610
MaeIII GTNAC 3 cut(s) 378, 1012, 1276
MalI GATC 3 cut(s) 33, 40, 209
MboI GATC 3 cut(s) 31, 38, 207
MboII GAAGA 5 cut(s) 365, 461, 717, 865, 1268
MmeI TCCRAC 1 cut(s) 1250
MseI TTAA 2 cut(s) 744, 822
MslI CAYNNNNRTG 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 1 cut(s) 692
MspR9I CCNGG 3 cut(s) 1008, 1058, 1163
MvaI CCWGG 3 cut(s) 1008, 1058, 1163
MwoI GCNNNNNNNGC 1 cut(s) 1017
NdeII GATC 3 cut(s) 31, 38, 207
NlaIII CATG 3 cut(s) 242, 331, 544
NlaIV GGNNCC 6 cut(s) 103, 221, 618, 696, 759, 1055
NspI RCATGY 2 cut(s) 331, 544
PciI ACATGT 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 799
PfeI GAWTC 1 cut(s) 905
PkrI GCNGC 3 cut(s) 415, 1046, 1151
PscI ACATGT 1 cut(s) 327
Psp6I CCWGG 3 cut(s) 1006, 1056, 1161
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 3 cut(s) 1006, 1056, 1161
PspN4I GGNNCC 6 cut(s) 103, 221, 618, 696, 759, 1055
PspPI GGNCC 1 cut(s) 671
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 6 cut(s) 344, 464, 489, 899, 1055, 1117
RsaNI GTAC 6 cut(s) 343, 463, 488, 898, 1054, 1116
RseI CAYNNNNRTG 1 cut(s) 545
SaqAI TTAA 2 cut(s) 744, 822
SatI GCNGC 3 cut(s) 414, 1045, 1150
Sau3AI GATC 3 cut(s) 31, 38, 207
Sau96I GGNCC 1 cut(s) 671
ScaI AGTACT 2 cut(s) 344, 489
ScrFI CCNGG 3 cut(s) 1008, 1058, 1163
SexAI ACCWGGT 1 cut(s) 1056
SfcI CTRYAG 3 cut(s) 253, 483, 1143
SinI GGWCC 1 cut(s) 671
SmiMI CAYNNNNRTG 1 cut(s) 545
SsiI CCGC 4 cut(s) 59, 111, 154, 620
SspMI CTAG 3 cut(s) 473, 956, 1094
StyD4I CCNGG 3 cut(s) 1006, 1056, 1161
StyI CCWWGG 1 cut(s) 1048
TaaI ACNGT 6 cut(s) 337, 347, 562, 595, 820, 1218
TaiI ACGT 2 cut(s) 13, 613
TaqI TCGA 3 cut(s) 303, 728, 1079
TatI WGTACW 3 cut(s) 342, 487, 1115
TfiI GAWTC 1 cut(s) 905
Tru1I TTAA 2 cut(s) 744, 822
Tru9I TTAA 2 cut(s) 744, 822
TscAI CASTG 3 cut(s) 126, 946, 993
TseI GCWGC 3 cut(s) 413, 1044, 1149
TspDTI ATGAA 2 cut(s) 798, 1119
TspGWI ACGGA 2 cut(s) 120, 185
TspRI CASTG 3 cut(s) 126, 946, 993
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 1 cut(s) 701
XceI RCATGY 2 cut(s) 331, 544
XcmI CCANNNNNNNNNTGG 1 cut(s) 985
XspI CTAG 3 cut(s) 473, 956, 1094
ZrmI AGTACT 2 cut(s) 344, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.