Rroxscaffold_7G00158700

branched-chain-amino-acid aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004977
Physical Location & Seq
Forward (+)
2044657 .. 2047706
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158700.1

Sequence Viewer

Length: 1203 bp
ATGGGTTCCACTTCCGCCCTCGCACTGGATTTTCCGTCCCCAATCGCAACTACTCTCCGCACCTCCAAAACTACCCTGATTCACCCCCACTCGTCGTCTTACAAGCAGCTCCGTCTCTGTTTGATCACCACAAGGCTCCAAGCCATTCAGTCTCATGCCTCTGGAAAGAACAGCCAAGCGTCTGAATTGCCTGATATAAGTTGGGACAATGTTGGCTTCGACAACCCTATGACTACCGATTATATGTTTACTATGAAGTGTTCTGAAGATGGGAACTTTTCCGATGGTGGGTTACAGCGTTTTGGTAAAATTGAGTTGAACCCAGCTGCCTGTGTTTTGAACTATGGTCAGGGAGTAATTGAAGAATTGAAAGCGTACAAGAAGCTAGATAATTCTTTAGTACTATTTCGTCCAGAGGAAAATGGATTGAGGATGAGAATGGGAGCAGAGCGCATGTGTATGCCTGCACCAACTGTTGAGCAGTTTGTGGAAGCTGTGAAACTTACTGTATTGGCAAATAGACGTTGGGTTCCTCCACCAAATAAAGGCTTTCTACATATCAAACCACTACTTATGGGGAATGGACCTGTTCTTAGTCTTACCCCGGCTCCAGAATTCACCTTTCTCATATATGCTTCTTCGATGGGGAACACCTTTAAGGGGGGACTGGAACCAATCAACTTAGTGGTTGAAAATGAAATCCATCGTGCAGTTCGTGGTGGAGCTGGAACTGTTAATGCCATAGGGAATTATGCTATGGTTTTGAAGACACAGGTGGAGGCAAAAGCAGATGGTTTCTCTGATGTTTTGTACCTTGATTCTGTTCACAAAAGATATGTGGAACAGACTTCCACTGCTAACATCTTTCTAGTGAAGGACAAAATTATCTCCACTCCAGCACTGGAAGGGACAATTTTGCTTGGCGTTACACGCAAAAGTATAATTGAAATTGCTCGCAGCCAAGGGTACCAGGTTGAGGAGCGCCTTGTGTCGATTGAGGAATTGCAAGAAGCTGATGAAGTGTTCTGTACAGGATATGCTGTCTGTTTATCACCAATAGGCAGCATAACTTACCTGGGAAAAAGGGTGTCTTACCAAGAAACTGGACTTGGTGGAGTTTCTGAGCAACTGTATCAGGCTCTTTCAAATATACAGATGGGTCTTGCAGAAGACAATAGGGGTTGGATAGTAACCTTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

400

Amino Acids

43.88

Weight (kDa)

6.14

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 136 - 356 1.4e-25 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 966
AccB1I GGYRCC 1 cut(s) 966
AciI CCGC 2 cut(s) 15, 58
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 1 cut(s) 285
AfaI GTAC 5 cut(s) 377, 402, 812, 968, 1030
AfiI CCNNNNNNNGG 5 cut(s) 25, 288, 545, 660, 976
AgsI TTSAA 9 cut(s) 319, 340, 362, 370, 692, 766, 947, 1146, 1198
AjnI CCWGG 2 cut(s) 969, 1074
AjuI GAANNNNNNNTTGG 2 cut(s) 1092, 1124
AluBI AGCT 6 cut(s) 109, 326, 385, 494, 725, 1013
AluI AGCT 6 cut(s) 109, 326, 385, 494, 725, 1013
Alw26I GTCTC 2 cut(s) 119, 156
ApeKI GCWGC 4 cut(s) 106, 326, 957, 1062
ApoI RAATTY 1 cut(s) 614
Asp718I GGTACC 1 cut(s) 966
AspLEI GCGC 2 cut(s) 453, 984
AspS9I GGNCC 1 cut(s) 584
AsuC2I CCSGG 1 cut(s) 605
AsuHPI GGTGA 4 cut(s) 74, 118, 610, 1044
AvaII GGWCC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 966
BbsI GAAGAC 2 cut(s) 773, 1176
BbvI GCAGC 4 cut(s) 118, 313, 969, 1074
BccI CCATC 6 cut(s) 263, 278, 637, 711, 785, 1150
BciT130I CCWGG 2 cut(s) 971, 1076
BclI TGATCA 1 cut(s) 123
BcnI CCSGG 1 cut(s) 605
BcoDI GTCTC 2 cut(s) 119, 156
BfaI CTAG 2 cut(s) 386, 869
BfoI RGCGCY 1 cut(s) 985
BisI GCNGC 4 cut(s) 107, 327, 958, 1063
BlsI GCNGC 4 cut(s) 108, 328, 959, 1064
BmcAI AGTACT 1 cut(s) 402
Bme1390I CCNGG 3 cut(s) 605, 971, 1076
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 6 cut(s) 7, 137, 531, 609, 672, 968
BmrFI CCNGG 3 cut(s) 605, 971, 1076
BpiI GAAGAC 2 cut(s) 773, 1176
BpmI CTGGAG 2 cut(s) 594, 879
BpuMI CCSGG 1 cut(s) 605
BsaJI CCNNGG 3 cut(s) 603, 961, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 592, 622
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 288, 545, 660, 976
Bse1I ACTGG 4 cut(s) 30, 672, 906, 1108
BseBI CCWGG 2 cut(s) 971, 1076
BseDI CCNNGG 3 cut(s) 603, 961, 1075
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 5 cut(s) 25, 288, 545, 660, 976
BseMII CTCAG 1 cut(s) 1113
BseNI ACTGG 4 cut(s) 30, 672, 906, 1108
BseRI GAGGAG 1 cut(s) 992
BseXI GCAGC 4 cut(s) 118, 313, 969, 1074
BseYI CCCAGC 1 cut(s) 322
BsgI GTGCAG 2 cut(s) 450, 729
BshNI GGYRCC 1 cut(s) 966
BsiSI CCGG 1 cut(s) 605
BslFI GGGAC 4 cut(s) 22, 218, 678, 922
BslI CCNNNNNNNGG 5 cut(s) 25, 288, 545, 660, 976
BsmAI GTCTC 2 cut(s) 119, 156
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 4 cut(s) 22, 218, 678, 922
Bsp1407I TGTACA 1 cut(s) 1028
Bsp143I GATC 1 cut(s) 123
BspACI CCGC 2 cut(s) 15, 58
BspCNI CTCAG 1 cut(s) 1114
BspLI GGNNCC 6 cut(s) 7, 137, 531, 609, 672, 968
BspT107I GGYRCC 1 cut(s) 966
BsrGI TGTACA 1 cut(s) 1028
BsrI ACTGG 4 cut(s) 30, 672, 906, 1108
BssECI CCNNGG 3 cut(s) 603, 961, 1075
BssMI GATC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 961
Bst2UI CCWGG 2 cut(s) 971, 1076
Bst4CI ACNGT 4 cut(s) 475, 508, 733, 1131
BstAUI TGTACA 1 cut(s) 1028
BstC8I GCNNGC 2 cut(s) 465, 955
BstDEI CTNAG 3 cut(s) 593, 682, 1122
BstF5I GGATG 1 cut(s) 438
BstH2I RGCGCY 1 cut(s) 985
BstHHI GCGC 2 cut(s) 453, 984
BstKTI GATC 1 cut(s) 126
BstMAI GTCTC 2 cut(s) 119, 156
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 930
BstNI CCWGG 2 cut(s) 971, 1076
BstNSI RCATGY 1 cut(s) 457
BstSCI CCNGG 3 cut(s) 603, 969, 1074
BstV1I GCAGC 4 cut(s) 118, 313, 969, 1074
BstV2I GAAGAC 2 cut(s) 773, 1176
BstXI CCANNNNNNTGG 1 cut(s) 1103
BtsCI GGATG 1 cut(s) 438
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 3 cut(s) 23, 852, 899
Cac8I GCNNGC 2 cut(s) 465, 955
CfoI GCGC 2 cut(s) 453, 984
Cfr13I GGNCC 1 cut(s) 584
CseI GACGC 1 cut(s) 168
CsiI ACCWGGT 1 cut(s) 969
Csp6I GTAC 5 cut(s) 376, 401, 811, 967, 1029
CviAII CATG 2 cut(s) 155, 454
CviQI GTAC 5 cut(s) 376, 401, 811, 967, 1029
DdeI CTNAG 3 cut(s) 593, 682, 1122
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 961
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 285
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 969, 1074
EcoT14I CCWWGG 1 cut(s) 961
ErhI CCWWGG 1 cut(s) 961
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 2 cut(s) 158, 457
FalI AAGNNNNNCTT 2 cut(s) 1075, 1107
FaqI GGGAC 4 cut(s) 22, 218, 678, 922
FatI CATG 2 cut(s) 154, 453
FbaI TGATCA 1 cut(s) 123
Fnu4HI GCNGC 4 cut(s) 107, 327, 958, 1063
FokI GGATG 1 cut(s) 445
Fsp4HI GCNGC 4 cut(s) 107, 327, 958, 1063
FspBI CTAG 2 cut(s) 386, 869
GlaI GCGC 2 cut(s) 452, 983
GluI GCNGC 4 cut(s) 107, 327, 958, 1063
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 2 cut(s) 594, 879
HaeII RGCGCY 1 cut(s) 985
HapII CCGG 1 cut(s) 605
HgaI GACGC 1 cut(s) 168
HhaI GCGC 2 cut(s) 453, 984
Hin1II CATG 2 cut(s) 158, 457
Hin6I GCGC 2 cut(s) 451, 982
HinP1I GCGC 2 cut(s) 451, 982
HinfI GANTC 2 cut(s) 79, 818
HpaII CCGG 1 cut(s) 605
HphI GGTGA 4 cut(s) 74, 118, 610, 1044
Hpy166II GTNNAC 2 cut(s) 249, 826
Hpy188I TCNGA 5 cut(s) 184, 265, 283, 802, 1123
Hpy188III TCNNGA 3 cut(s) 162, 413, 611
Hpy8I GTNNAC 2 cut(s) 249, 826
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 2 cut(s) 868, 899
HpyCH4III ACNGT 4 cut(s) 475, 508, 733, 1131
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 4 cut(s) 467, 710, 1006, 1166
HpyF10VI GCNNNNNNNGC 1 cut(s) 930
HpyF3I CTNAG 3 cut(s) 593, 682, 1122
HpySE526I ACGT 1 cut(s) 523
Hsp92II CATG 2 cut(s) 158, 457
HspAI GCGC 2 cut(s) 451, 982
KpnI GGTACC 1 cut(s) 970
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 6 cut(s) 114, 141, 443, 613, 722, 979
Lsp1109I GCAGC 4 cut(s) 118, 313, 969, 1074
MabI ACCWGGT 1 cut(s) 969
MaeI CTAG 2 cut(s) 386, 869
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 3 cut(s) 291, 925, 1189
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 5 cut(s) 278, 374, 630, 778, 1181
MmeI TCCRAC 1 cut(s) 1163
MnlI CCTC 9 cut(s) 29, 73, 169, 409, 423, 543, 772, 970, 991
MseI TTAA 2 cut(s) 657, 735
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 1 cut(s) 605
MspR9I CCNGG 3 cut(s) 605, 971, 1076
MvaI CCWGG 2 cut(s) 971, 1076
MwoI GCNNNNNNNGC 1 cut(s) 930
NciI CCSGG 1 cut(s) 605
NdeII GATC 1 cut(s) 123
NlaIII CATG 2 cut(s) 158, 457
NlaIV GGNNCC 6 cut(s) 7, 137, 531, 609, 672, 968
NspI RCATGY 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PfeI GAWTC 2 cut(s) 79, 818
PkrI GCNGC 4 cut(s) 108, 328, 959, 1064
Psp6I CCWGG 2 cut(s) 969, 1074
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 2 cut(s) 969, 1074
PspN4I GGNNCC 6 cut(s) 7, 137, 531, 609, 672, 968
PspPI GGNCC 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 5 cut(s) 377, 402, 812, 968, 1030
RsaNI GTAC 5 cut(s) 376, 401, 811, 967, 1029
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 2 cut(s) 657, 735
SatI GCNGC 4 cut(s) 107, 327, 958, 1063
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 584
ScaI AGTACT 1 cut(s) 402
ScrFI CCNGG 3 cut(s) 605, 971, 1076
SexAI ACCWGGT 1 cut(s) 969
SinI GGWCC 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 458
SsiI CCGC 2 cut(s) 15, 58
SspMI CTAG 2 cut(s) 386, 869
StyD4I CCNGG 3 cut(s) 603, 969, 1074
StyI CCWWGG 1 cut(s) 961
TaaI ACNGT 4 cut(s) 475, 508, 733, 1131
TaiI ACGT 1 cut(s) 526
TaqI TCGA 3 cut(s) 219, 641, 992
TatI WGTACW 2 cut(s) 400, 1028
TfiI GAWTC 2 cut(s) 79, 818
Tru1I TTAA 2 cut(s) 657, 735
Tru9I TTAA 2 cut(s) 657, 735
TscAI CASTG 3 cut(s) 30, 859, 906
TseI GCWGC 4 cut(s) 106, 326, 957, 1062
TspDTI ATGAA 3 cut(s) 269, 711, 1032
TspGWI ACGGA 2 cut(s) 24, 101
TspRI CASTG 3 cut(s) 30, 859, 906
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 1 cut(s) 614
XceI RCATGY 1 cut(s) 457
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XspI CTAG 2 cut(s) 386, 869
ZrmI AGTACT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.