RLG00000010495

branched-chain-amino-acid aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004977
Physical Location & Seq
Forward (+)
2118384 .. 2121685
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010495

Sequence Viewer

Length: 1332 bp
ATGAATTCGGATTCAGCGCTGGCCCAGACCAATAGTTTAACTGCTCTCACGTGCTTGTCTCGTGCCGCCCCTTGCACTGTTTCGGACATCCCAACCAAAACGGAAAAGACACAGATGGGTTCCACTTCCGCCCTCGCACTGGATTTTCCGTACCGAATCGCAACTACTCTCCGCACCTCCAAAACTACCCTTCACCCCCACTCCTCGTCTTACAAGGCACGCACTTGTTCAAGTGTTGTCCTCCGTCTCTGTTTGATCACCACAAGGCTCCAAGCCATTCAGTCTCATGCCTCTGGCTATAGCCAAGCGTCTGAATTGCCTGATATAAGTTGGGACAATGTTGGCTTCAACAACCCTATGACTACCGATTATATGTTTACTGTGAAGTGTTCTGAAGATGGGAACTTTTCCGATGGTGGGTTACAGCGTTTTGGTAAAATTGAGTTGAACCCAGCTGCCTGTGTTTTGAACTATGGTCAGGGAGTAATTGAAGAATTGAAAGCGTACAAGAAGCTAGATCATTCTATAGTACTATTTCGTCCAGAGGAAAATGGATTGAGGATGAGAATGGGAGCAGAGCGCATGTGTATGCCTGCACCAACTGTTGAGCAGTTTGTGGAAGCTGTGAAACTTACTGTATTGGCAAATAGACGTTGGGTTCCTCCACCAAATCAAGGCTTTCTACATATCAAACCACTACTTATGGGGAATGGACCTGTTCTTAGTCTTACACCAGCTCCAGAATTCACCTTTCTCATATATGCTTCTTCGATGGGGAACACCTTTAAGGGGGGACTGGAACCAATCAACTTAGTGGTTGAAAATGAAATCCATCGTGCAGTTTGTGGTGGAGCTGGAACTGTTAATGCCATAGGGAATTATGCTATGGTTTTGAAGACACAGGTGGAGGCAAAAGCAGATGGTTTCTCTGATGTTTTGTACCTTGATTCTGTTCACAAAAGATATGTGGAACAGACTTCCACTGCTAACATCTTTCTAGTGAAGGACAAAATTATCTCCACTCCAGCACTGGAAGGGACAATTTTGCCTGGCATTACACGCAAAAGTATAATTGAAATTGCTCGCAGCCAAGGGTACCAGGTTGAGGAGCGCCCTGTGTCGATTGAGGAATTGCTAGAAGCTGATGAAGTGTTCTGTACCGGATATACTGTCTGTTTATCACCTATAGGCAGCATAACTTACCTGGGAAAAAGGGTGTCTTACCAAGAAACTGGACTTGGTGGAGTTTCTGAGCAACTGTATCAGGTTCTTTCAAATATACAGATGGGTCTTGCAGAAGACAATAGGGGTTGGTTAGTAACCTTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

444

Amino Acids

48.41

Weight (kDa)

6.21

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 164 - 399 3.3e-27 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1095
AccB1I GGYRCC 1 cut(s) 1095
AciI CCGC 3 cut(s) 66, 129, 172
AcsI RAATTY 2 cut(s) 4, 743
AcuI CTGAAG 1 cut(s) 414
AcvI CACGTG 1 cut(s) 51
AfaI GTAC 6 cut(s) 152, 506, 531, 941, 1097, 1159
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 5 cut(s) 139, 417, 674, 789, 1105
AjnI CCWGG 3 cut(s) 1048, 1098, 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 1221, 1253
AluBI AGCT 6 cut(s) 455, 514, 623, 737, 854, 1142
AluI AGCT 6 cut(s) 455, 514, 623, 737, 854, 1142
Alw26I GTCTC 3 cut(s) 63, 251, 288
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 3 cut(s) 455, 1086, 1191
ApoI RAATTY 2 cut(s) 4, 743
Asp718I GGTACC 1 cut(s) 1095
AspLEI GCGC 3 cut(s) 19, 582, 1113
AspS9I GGNCC 2 cut(s) 22, 713
AsuHPI GGTGA 4 cut(s) 185, 250, 739, 1173
AvaII GGWCC 1 cut(s) 713
BanI GGYRCC 1 cut(s) 1095
BauI CACGAG 1 cut(s) 60
BbrPI CACGTG 1 cut(s) 51
BbsI GAAGAC 2 cut(s) 902, 1305
BbvI GCAGC 3 cut(s) 442, 1098, 1203
BccI CCATC 7 cut(s) 109, 392, 407, 766, 840, 914, 1279
BciT130I CCWGG 3 cut(s) 1050, 1100, 1205
BclI TGATCA 1 cut(s) 255
BcoDI GTCTC 3 cut(s) 63, 251, 288
BfaI CTAG 3 cut(s) 515, 998, 1136
BfmI CTRYAG 3 cut(s) 298, 525, 1185
BfoI RGCGCY 2 cut(s) 20, 1114
BisI GCNGC 4 cut(s) 66, 456, 1087, 1192
BlsI GCNGC 4 cut(s) 67, 457, 1088, 1193
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 3 cut(s) 1050, 1100, 1205
Bme18I GGWCC 1 cut(s) 713
BmgT120I GGNCC 2 cut(s) 22, 713
BmiI GGNNCC 5 cut(s) 121, 269, 660, 801, 1097
BmrFI CCNGG 3 cut(s) 1050, 1100, 1205
BpiI GAAGAC 2 cut(s) 902, 1305
BpmI CTGGAG 2 cut(s) 723, 1008
BsaAI YACGTR 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 1090, 1204
BsaWI WCCGGW 1 cut(s) 1160
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 721, 751
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 417, 674, 789, 1105
Bse1I ACTGG 4 cut(s) 144, 801, 1035, 1237
BseBI CCWGG 3 cut(s) 1050, 1100, 1205
BseDI CCNNGG 2 cut(s) 1090, 1204
BseGI GGATG 2 cut(s) 87, 567
BseLI CCNNNNNNNGG 5 cut(s) 139, 417, 674, 789, 1105
BseMII CTCAG 1 cut(s) 1242
BseNI ACTGG 4 cut(s) 144, 801, 1035, 1237
BseRI GAGGAG 2 cut(s) 193, 1121
BseXI GCAGC 3 cut(s) 442, 1098, 1203
BseYI CCCAGC 1 cut(s) 451
BsgI GTGCAG 2 cut(s) 579, 858
BshFI GGCC 1 cut(s) 23
BshNI GGYRCC 1 cut(s) 1095
BsiSI CCGG 1 cut(s) 1161
BslFI GGGAC 3 cut(s) 347, 807, 1051
BslI CCNNNNNNNGG 5 cut(s) 139, 417, 674, 789, 1105
BsmAI GTCTC 3 cut(s) 63, 251, 288
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 3 cut(s) 347, 807, 1051
BsnI GGCC 1 cut(s) 23
Bsp143I GATC 2 cut(s) 255, 517
BspACI CCGC 3 cut(s) 66, 129, 172
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 1243
BspLI GGNNCC 5 cut(s) 121, 269, 660, 801, 1097
BspT107I GGYRCC 1 cut(s) 1095
BsrI ACTGG 4 cut(s) 144, 801, 1035, 1237
BssECI CCNNGG 2 cut(s) 1090, 1204
BssMI GATC 2 cut(s) 255, 517
BssSI CACGAG 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 1090
Bst2BI CACGAG 1 cut(s) 60
Bst2UI CCWGG 3 cut(s) 1050, 1100, 1205
Bst4CI ACNGT 7 cut(s) 79, 382, 604, 637, 862, 1171, 1260
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 4 cut(s) 21, 220, 594, 1084
BstDEI CTNAG 3 cut(s) 722, 811, 1251
BstF5I GGATG 2 cut(s) 87, 567
BstH2I RGCGCY 2 cut(s) 20, 1114
BstHHI GCGC 3 cut(s) 19, 582, 1113
BstKTI GATC 2 cut(s) 258, 520
BstMAI GTCTC 3 cut(s) 63, 251, 288
BstMBI GATC 2 cut(s) 255, 517
BstMWI GCNNNNNNNGC 1 cut(s) 1059
BstNI CCWGG 3 cut(s) 1050, 1100, 1205
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 3 cut(s) 1048, 1098, 1203
BstSFI CTRYAG 3 cut(s) 298, 525, 1185
BstV1I GCAGC 3 cut(s) 442, 1098, 1203
BstV2I GAAGAC 2 cut(s) 902, 1305
BstXI CCANNNNNNTGG 1 cut(s) 1232
BsuRI GGCC 1 cut(s) 23
BtsCI GGATG 2 cut(s) 87, 567
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 4 cut(s) 75, 137, 981, 1028
Cac8I GCNNGC 4 cut(s) 21, 220, 594, 1084
CfoI GCGC 3 cut(s) 19, 582, 1113
Cfr13I GGNCC 2 cut(s) 22, 713
CseI GACGC 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 1098
Csp6I GTAC 6 cut(s) 151, 505, 530, 940, 1096, 1158
CviAII CATG 2 cut(s) 287, 583
CviQI GTAC 6 cut(s) 151, 505, 530, 940, 1096, 1158
DdeI CTNAG 3 cut(s) 722, 811, 1251
DpnI GATC 2 cut(s) 257, 519
DpnII GATC 2 cut(s) 255, 517
EciI GGCGGA 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 1090
Eco47I GGWCC 1 cut(s) 713
Eco47III AGCGCT 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 414
Eco72I CACGTG 1 cut(s) 51
EcoRI GAATTC 2 cut(s) 4, 743
EcoRII CCWGG 3 cut(s) 1048, 1098, 1203
EcoT14I CCWWGG 1 cut(s) 1090
ErhI CCWWGG 1 cut(s) 1090
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 2 cut(s) 290, 586
FalI AAGNNNNNCTT 2 cut(s) 1204, 1236
FaqI GGGAC 3 cut(s) 347, 807, 1051
FatI CATG 2 cut(s) 286, 582
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 4 cut(s) 66, 456, 1087, 1192
FokI GGATG 2 cut(s) 74, 574
Fsp4HI GCNGC 4 cut(s) 66, 456, 1087, 1192
FspBI CTAG 3 cut(s) 515, 998, 1136
GlaI GCGC 3 cut(s) 18, 581, 1112
GluI GCNGC 4 cut(s) 66, 456, 1087, 1192
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 2 cut(s) 723, 1008
HaeII RGCGCY 2 cut(s) 20, 1114
HaeIII GGCC 1 cut(s) 23
HapII CCGG 1 cut(s) 1161
HgaI GACGC 1 cut(s) 297
HhaI GCGC 3 cut(s) 19, 582, 1113
Hin1II CATG 2 cut(s) 290, 586
Hin6I GCGC 3 cut(s) 17, 580, 1111
HinP1I GCGC 3 cut(s) 17, 580, 1111
HinfI GANTC 3 cut(s) 11, 156, 947
HpaII CCGG 1 cut(s) 1161
HphI GGTGA 4 cut(s) 185, 250, 739, 1173
Hpy166II GTNNAC 2 cut(s) 378, 955
Hpy188I TCNGA 7 cut(s) 10, 85, 313, 394, 412, 931, 1252
Hpy188III TCNNGA 2 cut(s) 542, 740
Hpy8I GTNNAC 2 cut(s) 378, 955
HpyAV CCTTC 3 cut(s) 200, 997, 1028
HpyCH4III ACNGT 7 cut(s) 79, 382, 604, 637, 862, 1171, 1260
HpyCH4IV ACGT 2 cut(s) 50, 652
HpyCH4V TGCA 4 cut(s) 75, 596, 839, 1295
HpyF10VI GCNNNNNNNGC 1 cut(s) 1059
HpyF3I CTNAG 3 cut(s) 722, 811, 1251
HpySE526I ACGT 2 cut(s) 50, 652
Hsp92II CATG 2 cut(s) 290, 586
HspAI GCGC 3 cut(s) 17, 580, 1111
KpnI GGTACC 1 cut(s) 1099
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 2 cut(s) 255, 517
LmnI GCTCC 5 cut(s) 273, 572, 742, 851, 1108
Lsp1109I GCAGC 3 cut(s) 442, 1098, 1203
MabI ACCWGGT 1 cut(s) 1098
MaeI CTAG 3 cut(s) 515, 998, 1136
MaeII ACGT 2 cut(s) 50, 652
MaeIII GTNAC 2 cut(s) 420, 1318
MalI GATC 2 cut(s) 257, 519
MboI GATC 2 cut(s) 255, 517
MboII GAAGA 5 cut(s) 407, 503, 759, 907, 1310
MseI TTAA 3 cut(s) 38, 786, 864
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 1 cut(s) 1161
MspR9I CCNGG 3 cut(s) 1050, 1100, 1205
MvaI CCWGG 3 cut(s) 1050, 1100, 1205
MwoI GCNNNNNNNGC 1 cut(s) 1059
NdeII GATC 2 cut(s) 255, 517
NlaIII CATG 2 cut(s) 290, 586
NlaIV GGNNCC 5 cut(s) 121, 269, 660, 801, 1097
NspI RCATGY 1 cut(s) 586
PfeI GAWTC 3 cut(s) 11, 156, 947
PkrI GCNGC 4 cut(s) 67, 457, 1088, 1193
PmaCI CACGTG 1 cut(s) 51
PmlI CACGTG 1 cut(s) 51
Ppu21I YACGTR 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 1048, 1098, 1203
PspCI CACGTG 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 3 cut(s) 1048, 1098, 1203
PspN4I GGNNCC 5 cut(s) 121, 269, 660, 801, 1097
PspPI GGNCC 2 cut(s) 22, 713
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 6 cut(s) 152, 506, 531, 941, 1097, 1159
RsaNI GTAC 6 cut(s) 151, 505, 530, 940, 1096, 1158
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 3 cut(s) 38, 786, 864
SatI GCNGC 4 cut(s) 66, 456, 1087, 1192
Sau3AI GATC 2 cut(s) 255, 517
Sau96I GGNCC 2 cut(s) 22, 713
ScaI AGTACT 1 cut(s) 531
ScrFI CCNGG 3 cut(s) 1050, 1100, 1205
SexAI ACCWGGT 1 cut(s) 1098
SfcI CTRYAG 3 cut(s) 298, 525, 1185
SinI GGWCC 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 587
SsiI CCGC 3 cut(s) 66, 129, 172
SspMI CTAG 3 cut(s) 515, 998, 1136
StyD4I CCNGG 3 cut(s) 1048, 1098, 1203
StyI CCWWGG 1 cut(s) 1090
TaaI ACNGT 7 cut(s) 79, 382, 604, 637, 862, 1171, 1260
TaiI ACGT 2 cut(s) 53, 655
TaqI TCGA 2 cut(s) 770, 1121
TatI WGTACW 1 cut(s) 529
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 11, 156, 947
Tru1I TTAA 3 cut(s) 38, 786, 864
Tru9I TTAA 3 cut(s) 38, 786, 864
TscAI CASTG 4 cut(s) 82, 144, 988, 1035
TseI GCWGC 3 cut(s) 455, 1086, 1191
TspDTI ATGAA 3 cut(s) 17, 840, 1161
TspGWI ACGGA 3 cut(s) 116, 138, 233
TspRI CASTG 4 cut(s) 82, 144, 988, 1035
VpaK11BI GGWCC 1 cut(s) 713
XapI RAATTY 2 cut(s) 4, 743
XceI RCATGY 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 1027
XspI CTAG 3 cut(s) 515, 998, 1136
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.