FvH4_1g23810

dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004559
Physical Location & Seq
Forward (+)
15697595 .. 15698119
525 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23810.t1

Sequence Viewer

Length: 420 bp
ATGAAGTATTATGGCATGACAGAGCCTGGAAAGCAATTGGGCGTGGCAGGACTTGATGGACTTGGTCATATTGCTGTCAAAATTGGTAAGGCATTTGGTTTGAAGGTAACCGTCATCAGAACTTCCCCGGCTAAGGAGGAAGAAGCTATTAACATACTTGGGGCTGATTCTTTTGTTGTTTCAACTGACCCTGCACAACTGAAGGCAGCGATGGGAACCATGGATTACATAATTGACACAGTACCTGCAGTACATGCCCTTGCTCCATTAATTGGTCTACTTAACTTGAATGGCAAGCTGATCACTGTGGGTTTGCCTGATAAGCCAGTGGAGTTGCCTATTTTTCCATTGGTTCTAGGTACGTGTATATACCAACTAACAATTCCATATGTTCCGGGTTTTTTTTCCCTGTTCTTTTAA

Protein Analysis

140

Amino Acids

14.76

Weight (kDa)

6.05

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 20 - 119 2.2e-15 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 253
AccB7I CCANNNNNTGG 1 cut(s) 272
AccI GTMKAC 1 cut(s) 277
AcuI CTGAAG 1 cut(s) 221
AfaI GTAC 3 cut(s) 243, 252, 361
AfiI CCNNNNNNNGG 2 cut(s) 133, 272
AflIII ACRYGT 1 cut(s) 362
AgsI TTSAA 3 cut(s) 103, 183, 289
AjnI CCWGG 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AluBI AGCT 2 cut(s) 146, 298
AluI AGCT 2 cut(s) 146, 298
AlwNI CAGNNNCTG 2 cut(s) 26, 245
ApeKI GCWGC 1 cut(s) 206
AseI ATTAAT 1 cut(s) 269
AsuC2I CCSGG 2 cut(s) 128, 396
BbvI GCAGC 1 cut(s) 218
BccI CCATC 2 cut(s) 50, 205
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 2 cut(s) 128, 396
BfaI CTAG 1 cut(s) 356
BfmI CTRYAG 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 253
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 3 cut(s) 27, 128, 396
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 3 cut(s) 27, 128, 396
Bpu10I CCTNAGC 1 cut(s) 132
BpuMI CCSGG 2 cut(s) 128, 396
BsaAI YACGTR 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 126, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 272
Bse1I ACTGG 1 cut(s) 326
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 126, 219
BseLI CCNNNNNNNGG 2 cut(s) 133, 272
BseNI ACTGG 1 cut(s) 326
BseXI GCAGC 1 cut(s) 218
BsgI GTGCAG 1 cut(s) 177
BsiSI CCGG 2 cut(s) 128, 395
BslI CCNNNNNNNGG 2 cut(s) 133, 272
Bsp143I GATC 1 cut(s) 300
Bsp19I CCATGG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 1 cut(s) 250
BspMI ACCTGC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 126, 219
BssMI GATC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 112, 241, 307
BstAPI GCANNNNNTGC 1 cut(s) 254
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 1 cut(s) 132
BstDSI CCRYGG 1 cut(s) 219
BstEII GGTNACC 1 cut(s) 106
BstKTI GATC 1 cut(s) 303
BstMBI GATC 1 cut(s) 300
BstMWI GCNNNNNNNGC 3 cut(s) 31, 254, 322
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 257
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 3 cut(s) 25, 126, 394
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 1 cut(s) 218
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 303, 333
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 296
CaiI CAGNNNCTG 2 cut(s) 26, 245
Csp6I GTAC 3 cut(s) 242, 251, 360
CviAII CATG 3 cut(s) 16, 220, 254
CviJI RGCY 6 cut(s) 25, 131, 146, 164, 298, 325
CviKI_1 RGCY 6 cut(s) 25, 131, 146, 164, 298, 325
CviQI GTAC 3 cut(s) 242, 251, 360
DdeI CTNAG 1 cut(s) 132
DpnI GATC 1 cut(s) 302
DpnII GATC 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 221
Eco91I GGTNACC 1 cut(s) 106
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 19, 223, 257
FatI CATG 3 cut(s) 15, 219, 253
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 1 cut(s) 277
Fnu4HI GCNGC 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 1 cut(s) 356
GluI GCNGC 1 cut(s) 207
HapII CCGG 2 cut(s) 128, 395
Hin1II CATG 3 cut(s) 19, 223, 257
HinfI GANTC 1 cut(s) 167
HpaII CCGG 2 cut(s) 128, 395
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 97, 196
HpyCH4III ACNGT 3 cut(s) 112, 241, 307
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 2 cut(s) 194, 248
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 254, 322
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 3 cut(s) 19, 223, 257
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 1 cut(s) 300
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 9 cut(s) 12, 33, 39, 141, 204, 258, 330, 339, 408
Lsp1109I GCAGC 1 cut(s) 218
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 302
MboI GATC 1 cut(s) 300
MboII GAAGA 1 cut(s) 152
MfeI CAATTG 1 cut(s) 35
MluCI AATT 5 cut(s) 35, 81, 231, 270, 381
MnlI CCTC 1 cut(s) 130
MseI TTAA 4 cut(s) 150, 269, 282, 418
MspI CCGG 2 cut(s) 128, 395
MspR9I CCNGG 3 cut(s) 27, 128, 396
MunI CAATTG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 31, 254, 322
NciI CCSGG 2 cut(s) 128, 396
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 388
NdeII GATC 1 cut(s) 300
NlaIII CATG 3 cut(s) 19, 223, 257
NlaIV GGNNCC 1 cut(s) 217
NspI RCATGY 1 cut(s) 257
PfeI GAWTC 1 cut(s) 167
PflFI GACNNNGTC 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 1 cut(s) 208
Ppu21I YACGTR 1 cut(s) 363
PshBI ATTAAT 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 25
PspEI GGTNACC 1 cut(s) 106
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 217
PstI CTGCAG 1 cut(s) 250
PstNI CAGNNNCTG 2 cut(s) 26, 245
PsyI GACNNNGTC 1 cut(s) 63
RsaI GTAC 3 cut(s) 243, 252, 361
RsaNI GTAC 3 cut(s) 242, 251, 360
SaqAI TTAA 4 cut(s) 150, 269, 282, 418
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 1 cut(s) 300
ScrFI CCNGG 3 cut(s) 27, 128, 396
SetI ASST 6 cut(s) 108, 148, 247, 300, 361, 365
SfcI CTRYAG 1 cut(s) 246
Sse9I AATT 5 cut(s) 35, 81, 231, 270, 381
SspMI CTAG 1 cut(s) 356
StyD4I CCNGG 3 cut(s) 25, 126, 394
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 3 cut(s) 112, 241, 307
TaiI ACGT 1 cut(s) 365
TasI AATT 5 cut(s) 35, 81, 231, 270, 381
TatI WGTACW 1 cut(s) 250
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 4 cut(s) 150, 269, 282, 418
Tru9I TTAA 4 cut(s) 150, 269, 282, 418
TscAI CASTG 2 cut(s) 310, 333
TseI GCWGC 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 310, 333
Tth111I GACNNNGTC 1 cut(s) 63
Van91I CCANNNNNTGG 1 cut(s) 272
VspI ATTAAT 1 cut(s) 269
XceI RCATGY 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 277
XspI CTAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.