Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004559

Physical Location & Seq

Genomic Coordinates Reverse (-)

39888406 .. 39892327

Gene Structure 3922 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1280800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGCAATATCACCAGAAGAACACCCACAGAAGGCATTTGGGTGGGCTGCCAGAGACTCCTCTGGAATACTTTCCCCCTTCCGTTTCTCCCGAAGGGAAAATGCTGATGATGATGTAACAATAAAGGTCCTCTACTGTGGGGTTTGCCATTCAGACTTACATTCTGCCAAGAACGATTGGGGTTTCACCAACTACCCTGTTGTGCCGGGGCATGAAATTGTTGGTGTTGTGACCAAAACCGGGAACAATGTGGACAAATTCAAAGTAGGTGATCGTGTAGGAGTTGGGGTCATAGTGGGTTCCTGCATGAAATGTGAGACATGCCAACAGGACTTGGAGAATTATTGCCCTCGAACAATATTTACCTATAACTCACACTATCATGACCAAACAAAAACTTATGGTGGTTATTCTGATATAATTGTTGTTCACCATCGCTATGTTCTCCGCTTTCCTGATAACTTAGCCCCTGATGCGGGTGCACCACTTTTATGCGCTGGGATCACTGTGTACAGTCCAATGAAGTATTATGGCATGACTGAGCCCGGAAAGCATTTGGGTGTGGCGGGACTTGGTGGGCTTGGTCACATTGCTGTGAAGATTGGCAAGGCATTTGGTTTGAAAGTGACCGTCATCAGTTCCTCCCCAGCAAAGGAGGATGAGGCAACTAAGAGACTCGGGGCTGATTCTTTTCTCCTCTTAAGTGACCCTGCCAAATTGAAGGGAGCAATGGGTACCATGGATTACATCATTGACACAGTGTCTGCAGTTCATGCTCTGGCTCCATTAATTGGTCTACTGAAGCTGAACGGGAAGCTGGTCACCGTGGGTTTGCCTAACAAGCCCCTGGAGTTGCCTATCTTCCCTTTAGTTTTGGGGCGGAAGCTTGTAGGGGGAAGTGACATAGGAGGGGTGAAAGAGACGCAGGAGATGCTTGACTTTTGTGCAAAGCACAACATTACCTCAGACATCGAGCTGATTCATATGGATTTCATTAACACAGCAATGGAGCGACTTGCCAAATCAGATGTCAGGTATCGGTTTGTGATCGATGTAGCAAACTCCTTGACTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009699 GO:0009719 GO:0009725 GO:0009735 GO:0009808 GO:0009809 GO:0009987 GO:0010033 GO:0016491 GO:0016614 GO:0016616 GO:0019438 GO:0019748 GO:0042221 GO:0044237 GO:0044249 GO:0044550 GO:0045551 GO:0048046 GO:0050896 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

359

Amino Acids

38.91

Weight (kDa)

6.89

Isoelectric Point (pI)

29.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	36 - 153	3e-27	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	193 - 316	1.5e-19	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004559)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21730 AT2G21890 AT4G39330 AT4G39330
fragaria_vesca	FvH4_1g23790 FvH4_1g23790 FvH4_1g23810
malus_domestica	MD07G1183600.v1.1 MD13G1280800.v1.1
prunus_persica	Prupe.6G161800_v2.0.a1 Prupe.6G162000_v2.0.a1 Prupe.6G162000_v2.0.a1
pyrus_communis	pycom15g30090
rosa_chinensis	RchiOBHm_Chr2g0119681 RchiOBHm_Chr2g0119731
rosa_laevigata	RLG00000018484 RLG00000018488
rosa_multiflora	Rmu_sc0001501.1_g000063 Rmu_sc0001501.1_g000064 Rmu_sc0001501.1_g000065 Rmu_sc0001501.1_g000091 Rmu_sc0018654.1_g000001
rosa_roxburghii	Rroxscaffold_2G00124130 Rroxscaffold_2G00124200
rosa_rugosa	Rorug02G0222900 Rorug02G0223200
rosa_samantha	Rh2BG292100 Rh2BG292400
rosa_wichuraiana	Rw0G003780 Rw2G022420 Rw2G022450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	734
AccB1I	GGYRCC	1 cut(s)	734
AccB7I	CCANNNNNTGG	1 cut(s)	791
AccI	GTMKAC	1 cut(s)	796
AciI	CCGC	4 cut(s)	448, 476, 566, 880
AclWI	GGATC	1 cut(s)	509
AcsI	RAATTY	1 cut(s)	257
AcuI	CTGAAG	1 cut(s)	821
AfaI	GTAC	2 cut(s)	512, 736
AfiI	CCNNNNNNNGG	6 cut(s)	31, 240, 475, 476, 652, 791
AflII	CTTAAG	1 cut(s)	700
AgsI	TTSAA	3 cut(s)	262, 622, 721
AhdI	GACNNNNNGTC	1 cut(s)	760
AjnI	CCWGG	1 cut(s)	846
AluBI	AGCT	4 cut(s)	805, 817, 886, 976
AluI	AGCT	4 cut(s)	805, 817, 886, 976
Alw21I	GWGCWC	1 cut(s)	484
Alw26I	GTCTC	4 cut(s)	48, 311, 667, 914
Alw44I	GTGCAC	1 cut(s)	480
AlwI	GGATC	1 cut(s)	509
AlwNI	CAGNNNCTG	1 cut(s)	764
Ama87I	CYCGRG	1 cut(s)	677
ApaLI	GTGCAC	1 cut(s)	480
ApeKI	GCWGC	1 cut(s)	47
ApoI	RAATTY	1 cut(s)	257
ArsI	GACNNNNNNTTYG	2 cut(s)	1014, 1046
AseI	ATTAAT	1 cut(s)	788
Asp700I	GAANNNNTTC	1 cut(s)	70
Asp718I	GGTACC	1 cut(s)	734
AspLEI	GCGC	1 cut(s)	497
AspS9I	GGNCC	1 cut(s)	127
AsuC2I	CCSGG	3 cut(s)	207, 241, 546
AsuHPI	GGTGA	6 cut(s)	3, 178, 281, 422, 814, 925
AvaI	CYCGRG	1 cut(s)	677
AvaII	GGWCC	1 cut(s)	127
BaeGI	GKGCMC	1 cut(s)	484
BanI	GGYRCC	1 cut(s)	734
BanII	GRGCYC	1 cut(s)	546
Bbv12I	GWGCWC	1 cut(s)	484
BbvI	GCAGC	1 cut(s)	34
BccI	CCATC	1 cut(s)	441
BciT130I	CCWGG	1 cut(s)	848
BcnI	CCSGG	3 cut(s)	207, 241, 546
BcoDI	GTCTC	4 cut(s)	48, 311, 667, 914
BfmI	CTRYAG	1 cut(s)	765
BfrI	CTTAAG	1 cut(s)	700
BisI	GCNGC	1 cut(s)	48
BlsI	GCNGC	1 cut(s)	49
Bme1390I	CCNGG	4 cut(s)	207, 241, 546, 848
Bme18I	GGWCC	1 cut(s)	127
BmeRI	GACNNNNNGTC	1 cut(s)	760
BmeT110I	CYCGRG	1 cut(s)	677
BmgT120I	GGNCC	1 cut(s)	127
BmiI	GGNNCC	3 cut(s)	301, 736, 783
BmrFI	CCNGG	4 cut(s)	207, 241, 546, 848
BmsI	GCATC	2 cut(s)	463, 921
BpmI	CTGGAG	1 cut(s)	869
BpuMI	CCSGG	3 cut(s)	207, 241, 546
Bsa29I	ATCGAT	1 cut(s)	1050
BsaJI	CCNNGG	4 cut(s)	206, 738, 825, 846
BsaXI	ACNNNNNCTCC	2 cut(s)	273, 303
Bsc4I	CCNNNNNNNGG	6 cut(s)	31, 240, 475, 476, 652, 791
Bse3DI	GCAATG	3 cut(s)	588, 735, 1011
BseBI	CCWGG	1 cut(s)	848
BseCI	ATCGAT	1 cut(s)	1050
BseDI	CCNNGG	4 cut(s)	206, 738, 825, 846
BseGI	GGATG	1 cut(s)	664
BseLI	CCNNNNNNNGG	6 cut(s)	31, 240, 475, 476, 652, 791
BseMI	GCAATG	3 cut(s)	588, 735, 1011
BseMII	CTCAG	2 cut(s)	531, 978
BseRI	GAGGAG	2 cut(s)	49, 686
BseSI	GKGCMC	1 cut(s)	484
BseXI	GCAGC	1 cut(s)	34
BseYI	CCCAGC	2 cut(s)	497, 646
BshNI	GGYRCC	1 cut(s)	734
BshVI	ATCGAT	1 cut(s)	1050
BsiHKAI	GWGCWC	1 cut(s)	484
BsiHKCI	CYCGRG	1 cut(s)	677
BsiSI	CCGG	3 cut(s)	206, 240, 546
BslFI	GGGAC	1 cut(s)	582
BslI	CCNNNNNNNGG	6 cut(s)	31, 240, 475, 476, 652, 791
BsmAI	GTCTC	4 cut(s)	48, 311, 667, 914
BsmBI	CGTCTC	1 cut(s)	914
BsmFI	GGGAC	1 cut(s)	582
BsoBI	CYCGRG	1 cut(s)	677
Bsp1286I	GDGCHC	2 cut(s)	484, 546
Bsp1407I	TGTACA	1 cut(s)	510
Bsp143I	GATC	3 cut(s)	271, 501, 1047
Bsp19I	CCATGG	1 cut(s)	738
BspACI	CCGC	4 cut(s)	448, 476, 566, 880
BspCNI	CTCAG	2 cut(s)	532, 977
BspDI	ATCGAT	1 cut(s)	1050
BspHI	TCATGA	1 cut(s)	382
BspLI	GGNNCC	3 cut(s)	301, 736, 783
BspMAI	CTGCAG	1 cut(s)	769
BspPI	GGATC	1 cut(s)	509
BspT107I	GGYRCC	1 cut(s)	734
BspTI	CTTAAG	1 cut(s)	700
BsrDI	GCAATG	3 cut(s)	588, 735, 1011
BsrGI	TGTACA	1 cut(s)	510
BssECI	CCNNGG	4 cut(s)	206, 738, 825, 846
BssMI	GATC	3 cut(s)	271, 501, 1047
BssT1I	CCWWGG	1 cut(s)	738
Bst2UI	CCWGG	1 cut(s)	848
Bst4CI	ACNGT	6 cut(s)	137, 508, 515, 631, 760, 826
BstAFI	CTTAAG	1 cut(s)	700
BstAPI	GCANNNNNTGC	2 cut(s)	773, 931
BstAUI	TGTACA	1 cut(s)	510
BstDEI	CTNAG	5 cut(s)	463, 540, 669, 964, 1071
BstDSI	CCRYGG	2 cut(s)	738, 825
BstEII	GGTNACC	1 cut(s)	820
BstF5I	GGATG	1 cut(s)	664
BstHHI	GCGC	1 cut(s)	497
BstKTI	GATC	3 cut(s)	274, 504, 1050
BstMAI	GTCTC	4 cut(s)	48, 311, 667, 914
BstMBI	GATC	3 cut(s)	271, 501, 1047
BstMWI	GCNNNNNNNGC	5 cut(s)	473, 550, 773, 841, 931
BstNI	CCWGG	1 cut(s)	848
BstNSI	RCATGY	1 cut(s)	324
BstPI	GGTNACC	1 cut(s)	820
BstSCI	CCNGG	4 cut(s)	205, 239, 544, 846
BstSFI	CTRYAG	1 cut(s)	765
BstSLI	GKGCMC	1 cut(s)	484
BstV1I	GCAGC	1 cut(s)	34
Bsu15I	ATCGAT	1 cut(s)	1050
BsuTUI	ATCGAT	1 cut(s)	1050
BtgI	CCRYGG	2 cut(s)	738, 825
BtgZI	GCGATG	1 cut(s)	419
BtsCI	GGATG	1 cut(s)	664
BtsIMutI	CAGTG	2 cut(s)	504, 765
CaiI	CAGNNNCTG	1 cut(s)	764
CciI	TCATGA	1 cut(s)	382
CfoI	GCGC	1 cut(s)	497
Cfr13I	GGNCC	1 cut(s)	127
ClaI	ATCGAT	1 cut(s)	1050
CseI	GACGC	1 cut(s)	931
Csp6I	GTAC	2 cut(s)	511, 735
CviAII	CATG	7 cut(s)	212, 307, 321, 383, 535, 739, 773
CviQI	GTAC	2 cut(s)	511, 735
DdeI	CTNAG	5 cut(s)	463, 540, 669, 964, 1071
DpnI	GATC	3 cut(s)	273, 503, 1049
DpnII	GATC	3 cut(s)	271, 501, 1047
DriI	GACNNNNNGTC	1 cut(s)	760
Eam1105I	GACNNNNNGTC	1 cut(s)	760
EciI	GGCGGA	1 cut(s)	895
Eco130I	CCWWGG	1 cut(s)	738
Eco24I	GRGCYC	1 cut(s)	546
Eco47I	GGWCC	1 cut(s)	127
Eco57I	CTGAAG	1 cut(s)	821
Eco88I	CYCGRG	1 cut(s)	677
Eco91I	GGTNACC	1 cut(s)	820
EcoO109I	RGGNCCY	1 cut(s)	127
EcoO65I	GGTNACC	1 cut(s)	820
EcoRII	CCWGG	1 cut(s)	846
EcoT14I	CCWWGG	1 cut(s)	738
EcoT38I	GRGCYC	1 cut(s)	546
ErhI	CCWWGG	1 cut(s)	738
Esp3I	CGTCTC	1 cut(s)	914
FaeI	CATG	7 cut(s)	215, 310, 324, 386, 538, 742, 776
FaqI	GGGAC	1 cut(s)	582
FatI	CATG	7 cut(s)	211, 306, 320, 382, 534, 738, 772
FauI	CCCGC	2 cut(s)	469, 559
FauNDI	CATATG	1 cut(s)	984
FblI	GTMKAC	1 cut(s)	796
Fnu4HI	GCNGC	1 cut(s)	48
FokI	GGATG	1 cut(s)	671
FriOI	GRGCYC	1 cut(s)	546
Fsp4HI	GCNGC	1 cut(s)	48
GlaI	GCGC	1 cut(s)	496
GluI	GCNGC	1 cut(s)	48
GsaI	CCCAGC	2 cut(s)	501, 650
GsuI	CTGGAG	1 cut(s)	869
HapII	CCGG	3 cut(s)	206, 240, 546
HgaI	GACGC	1 cut(s)	931
HhaI	GCGC	1 cut(s)	497
Hin1II	CATG	7 cut(s)	215, 310, 324, 386, 538, 742, 776
Hin6I	GCGC	1 cut(s)	495
HinP1I	GCGC	1 cut(s)	495
HindIII	AAGCTT	1 cut(s)	884
HinfI	GANTC	5 cut(s)	56, 675, 686, 979, 1069
HpaII	CCGG	3 cut(s)	206, 240, 546
HphI	GGTGA	6 cut(s)	3, 178, 281, 422, 814, 925
Hpy166II	GTNNAC	5 cut(s)	253, 430, 482, 511, 797
Hpy188I	TCNGA	4 cut(s)	154, 415, 967, 1027
Hpy188III	TCNNGA	4 cut(s)	63, 90, 383, 455
Hpy8I	GTNNAC	5 cut(s)	253, 430, 482, 511, 797
HpyAV	CCTTC	4 cut(s)	25, 87, 88, 715
HpyCH4III	ACNGT	6 cut(s)	137, 508, 515, 631, 760, 826
HpyCH4V	TGCA	4 cut(s)	306, 482, 767, 947
HpyF10VI	GCNNNNNNNGC	5 cut(s)	473, 550, 773, 841, 931
HpyF3I	CTNAG	5 cut(s)	463, 540, 669, 964, 1071
Hsp92II	CATG	7 cut(s)	215, 310, 324, 386, 538, 742, 776
HspAI	GCGC	1 cut(s)	495
KpnI	GGTACC	1 cut(s)	738
Kzo9I	GATC	3 cut(s)	271, 501, 1047
LmnI	GCTCC	3 cut(s)	725, 787, 1009
Lsp1109I	GCAGC	1 cut(s)	34
LweI	GCATC	2 cut(s)	463, 921
MaeIII	GTNAC	7 cut(s)	115, 229, 584, 625, 704, 820, 899
MalI	GATC	3 cut(s)	273, 503, 1049
MboI	GATC	3 cut(s)	271, 501, 1047
MboII	GAAGA	3 cut(s)	29, 610, 853
MhlI	GDGCHC	2 cut(s)	484, 546
MluCI	AATT	6 cut(s)	216, 257, 340, 420, 716, 789
MlyI	GAGTC	3 cut(s)	50, 669, 1063
MnlI	CCTC	9 cut(s)	70, 140, 360, 649, 652, 655, 707, 902, 973
MroXI	GAANNNNTTC	1 cut(s)	70
MseI	TTAA	3 cut(s)	701, 788, 996
MslI	CAYNNNNRTG	7 cut(s)	40, 381, 438, 490, 558, 593, 1004
MspCI	CTTAAG	1 cut(s)	700
MspI	CCGG	3 cut(s)	206, 240, 546
MspR9I	CCNGG	4 cut(s)	207, 241, 546, 848
MvaI	CCWGG	1 cut(s)	848
MwoI	GCNNNNNNNGC	5 cut(s)	473, 550, 773, 841, 931
NciI	CCSGG	3 cut(s)	207, 241, 546
NcoI	CCATGG	1 cut(s)	738
NdeI	CATATG	1 cut(s)	984
NdeII	GATC	3 cut(s)	271, 501, 1047
NlaIII	CATG	7 cut(s)	215, 310, 324, 386, 538, 742, 776
NlaIV	GGNNCC	3 cut(s)	301, 736, 783
NmuCI	GTSAC	6 cut(s)	229, 584, 625, 704, 820, 899
NspI	RCATGY	1 cut(s)	324
PagI	TCATGA	1 cut(s)	382
PdmI	GAANNNNTTC	1 cut(s)	70
PfeI	GAWTC	2 cut(s)	686, 979
PflMI	CCANNNNNTGG	1 cut(s)	791
PkrI	GCNGC	1 cut(s)	49
PleI	GAGTC	3 cut(s)	50, 669, 1063
PpsI	GAGTC	3 cut(s)	50, 669, 1063
PpuMI	RGGWCCY	1 cut(s)	127
PshBI	ATTAAT	1 cut(s)	788
Psp5II	RGGWCCY	1 cut(s)	127
Psp6I	CCWGG	1 cut(s)	846
PspEI	GGTNACC	1 cut(s)	820
PspFI	CCCAGC	2 cut(s)	497, 646
PspGI	CCWGG	1 cut(s)	846
PspN4I	GGNNCC	3 cut(s)	301, 736, 783
PspPI	GGNCC	1 cut(s)	127
PspPPI	RGGWCCY	1 cut(s)	127
PsrI	GAACNNNNNNTAC	2 cut(s)	346, 378
PstI	CTGCAG	1 cut(s)	769
PstNI	CAGNNNCTG	1 cut(s)	764
RsaI	GTAC	2 cut(s)	512, 736
RsaNI	GTAC	2 cut(s)	511, 735
RseI	CAYNNNNRTG	7 cut(s)	40, 381, 438, 490, 558, 593, 1004
SaqAI	TTAA	3 cut(s)	701, 788, 996
SatI	GCNGC	1 cut(s)	48
Sau3AI	GATC	3 cut(s)	271, 501, 1047
Sau96I	GGNCC	1 cut(s)	127
SchI	GAGTC	3 cut(s)	50, 669, 1063
ScrFI	CCNGG	4 cut(s)	207, 241, 546, 848
SduI	GDGCHC	2 cut(s)	484, 546
SetI	ASST	9 cut(s)	129, 271, 368, 807, 819, 888, 965, 978, 1037
SfaNI	GCATC	2 cut(s)	463, 921
SfcI	CTRYAG	1 cut(s)	765
SinI	GGWCC	1 cut(s)	127
SmiMI	CAYNNNNRTG	7 cut(s)	40, 381, 438, 490, 558, 593, 1004
SmlI	CTYRAG	1 cut(s)	700
SmoI	CTYRAG	1 cut(s)	700
Sse9I	AATT	6 cut(s)	216, 257, 340, 420, 716, 789
SsiI	CCGC	4 cut(s)	448, 476, 566, 880
SspI	AATATT	1 cut(s)	360
StyD4I	CCNGG	4 cut(s)	205, 239, 544, 846
StyI	CCWWGG	1 cut(s)	738
TaaI	ACNGT	6 cut(s)	137, 508, 515, 631, 760, 826
TaqI	TCGA	3 cut(s)	352, 972, 1050
TasI	AATT	6 cut(s)	216, 257, 340, 420, 716, 789
TatI	WGTACW	1 cut(s)	510
TfiI	GAWTC	2 cut(s)	686, 979
Tru1I	TTAA	3 cut(s)	701, 788, 996
Tru9I	TTAA	3 cut(s)	701, 788, 996
TscAI	CASTG	2 cut(s)	511, 765
TseFI	GTSAC	6 cut(s)	229, 584, 625, 704, 820, 899
TseI	GCWGC	1 cut(s)	47
Tsp45I	GTSAC	6 cut(s)	229, 584, 625, 704, 820, 899
TspDTI	ATGAA	6 cut(s)	228, 323, 536, 761, 971, 982
TspGWI	ACGGA	1 cut(s)	71
TspRI	CASTG	2 cut(s)	511, 765
Van91I	CCANNNNNTGG	1 cut(s)	791
Vha464I	CTTAAG	1 cut(s)	700
VneI	GTGCAC	1 cut(s)	480
VpaK11BI	GGWCC	1 cut(s)	127
VspI	ATTAAT	1 cut(s)	788
XapI	RAATTY	1 cut(s)	257
XceI	RCATGY	1 cut(s)	324
XmiI	GTMKAC	1 cut(s)	796
XmnI	GAANNNNTTC	1 cut(s)	70

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.