Rh2BG292100

dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004559
Physical Location & Seq
Forward (+)
32858574 .. 32861903
3330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG292100.1

Sequence Viewer

Length: 786 bp
ATGGTGGGATCCTGCAACACATGTGAGACTTGCCAGCAGGACTTGGAGAACTATTGCCCGAAAGTGATATTCACCTATAACTCCCTCAATCATGACAAAACTAAGACTTATGGTGGTTATTCTGATATGATTGTTGTTGACCATCGCTTTGTGCTCCGCTTTCCCGATAACTTACCCCCAGATTCTGGCGCTCCACTTCTATGTGCCGGGATCACTGTGTATAGTCCAATGAAGTATTACGGCATGACAGAGCCTGGAAAGCATTTGGGCGTGGCAGGACTAGGTGGACTAGGTCATATTGCTGTCAAAATTGGTAAGGCTTTTGGTTTGAAGGTAACTGTCATCAGTACTTCCCCAGCTAAGGAGGATGAGGCTATTAACAGACTTGGGGCTGATTCTTTTGTTGTTTCAACTGACCCTGCACAATTGAAGGCAGCGATGGGTACCATGGATTACATAATTGACACAGTATCTGCAGTTCATGCCCTTGCTCCATTAATTGGTCTACTTAAGCTGAATGGGAAGCTGGTTACTGTGGGTTTGCCTAATAAGCCACTGGAGTTGCCTATATTTCCATTGGTTTTGGGGAGGAAGCTCGTTGGTGGAAGCGACGTTGGAGGGATAAAAGAGACGCAGGAGATGCTTGACTTTTGTGCCAAACACAATATTACCTCCGAAATCGAGCTAATTAAAATGGACTACATTAACACAGCTATGGAGCGACTTGCCAATTCAGATGTCAGGTATCGGTTTGTGATCGATGTGGGGAACTCCTCGTCTCAGTAG

Protein Analysis

261

Amino Acids

28.16

Weight (kDa)

6.07

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 96 - 219 1.1e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 443
AccB1I GGYRCC 1 cut(s) 443
AccB7I CCANNNNNTGG 2 cut(s) 185, 500
AccI GTMKAC 1 cut(s) 505
AciI CCGC 1 cut(s) 157
AclWI GGATC 3 cut(s) 3, 16, 218
AfaI GTAC 2 cut(s) 349, 445
AfiI CCNNNNNNNGG 3 cut(s) 185, 361, 500
AflII CTTAAG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 3 cut(s) 331, 411, 430
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 6 cut(s) 359, 514, 526, 595, 685, 713
AluI AGCT 6 cut(s) 359, 514, 526, 595, 685, 713
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 2 cut(s) 20, 623
AlwI GGATC 3 cut(s) 3, 16, 218
AlwNI CAGNNNCTG 3 cut(s) 185, 254, 473
ApeKI GCWGC 1 cut(s) 434
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 1 cut(s) 497
Asp718I GGTACC 1 cut(s) 443
AspLEI GCGC 1 cut(s) 191
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 1 cut(s) 64
BamHI GGATCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 446
BccI CCATC 2 cut(s) 150, 433
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 1 cut(s) 255
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 20, 623
BfaI CTAG 2 cut(s) 281, 290
BfmI CTRYAG 1 cut(s) 474
BfoI RGCGCY 1 cut(s) 192
BfrI CTTAAG 1 cut(s) 509
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 2 cut(s) 208, 255
BmiI GGNNCC 2 cut(s) 10, 445
BmrFI CCNGG 2 cut(s) 208, 255
BmsI GCATC 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 208
Bsa29I ATCGAT 1 cut(s) 759
BsaJI CCNNGG 1 cut(s) 447
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 361, 500
Bse1I ACTGG 1 cut(s) 561
BseBI CCWGG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 759
BseDI CCNNGG 1 cut(s) 447
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 3 cut(s) 185, 361, 500
BseNI ACTGG 1 cut(s) 561
BseRI GAGGAG 1 cut(s) 763
BseXI GCAGC 1 cut(s) 446
BseYI CCCAGC 1 cut(s) 355
BsgI GTGCAG 1 cut(s) 405
BshNI GGYRCC 1 cut(s) 443
BshVI ATCGAT 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 156
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 3 cut(s) 185, 361, 500
BsmAI GTCTC 2 cut(s) 20, 623
BsmBI CGTCTC 1 cut(s) 623
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 3 cut(s) 8, 210, 756
Bsp19I CCATGG 1 cut(s) 447
BspACI CCGC 1 cut(s) 157
BspDI ATCGAT 1 cut(s) 759
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 10, 445
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 3 cut(s) 3, 16, 218
BspT107I GGYRCC 1 cut(s) 443
BspTI CTTAAG 1 cut(s) 509
BsrI ACTGG 1 cut(s) 561
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 3 cut(s) 8, 210, 756
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 4 cut(s) 217, 340, 469, 535
BstAFI CTTAAG 1 cut(s) 509
BstAPI GCANNNNNTGC 2 cut(s) 482, 640
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 3 cut(s) 102, 360, 780
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 3 cut(s) 11, 213, 759
BstMAI GTCTC 2 cut(s) 20, 623
BstMBI GATC 3 cut(s) 8, 210, 756
BstMWI GCNNNNNNNGC 4 cut(s) 259, 482, 550, 640
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 2 cut(s) 206, 253
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
Bsu15I ATCGAT 1 cut(s) 759
BsuTUI ATCGAT 1 cut(s) 759
BtgI CCRYGG 1 cut(s) 447
BtgZI GCGATG 2 cut(s) 128, 452
BtsCI GGATG 1 cut(s) 373
BtsIMutI CAGTG 2 cut(s) 213, 554
Cac8I GCNNGC 1 cut(s) 35
CaiI CAGNNNCTG 3 cut(s) 185, 254, 473
CciI TCATGA 1 cut(s) 91
CfoI GCGC 1 cut(s) 191
ClaI ATCGAT 1 cut(s) 759
CseI GACGC 1 cut(s) 640
Csp6I GTAC 2 cut(s) 348, 444
CspCI CAANNNNNGTGG 2 cut(s) 543, 578
CviAII CATG 5 cut(s) 21, 92, 244, 448, 482
CviQI GTAC 2 cut(s) 348, 444
DdeI CTNAG 3 cut(s) 102, 360, 780
DpnI GATC 3 cut(s) 10, 212, 758
DpnII GATC 3 cut(s) 8, 210, 756
Eco130I CCWWGG 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 623
FaeI CATG 5 cut(s) 24, 95, 247, 451, 485
FatI CATG 5 cut(s) 20, 91, 243, 447, 481
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 2 cut(s) 281, 290
GlaI GCGC 1 cut(s) 190
GluI GCNGC 1 cut(s) 435
GsaI CCCAGC 1 cut(s) 359
GsuI CTGGAG 1 cut(s) 578
HaeII RGCGCY 1 cut(s) 192
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 640
HhaI GCGC 1 cut(s) 191
Hin1II CATG 5 cut(s) 24, 95, 247, 451, 485
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 2 cut(s) 182, 395
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 3 cut(s) 139, 287, 506
Hpy188I TCNGA 3 cut(s) 124, 676, 736
Hpy188III TCNNGA 2 cut(s) 92, 164
Hpy8I GTNNAC 3 cut(s) 139, 287, 506
Hpy99I CGWCG 1 cut(s) 614
HpyAV CCTTC 2 cut(s) 325, 424
HpyCH4III ACNGT 4 cut(s) 217, 340, 469, 535
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 3 cut(s) 15, 422, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 259, 482, 550, 640
HpyF3I CTNAG 3 cut(s) 102, 360, 780
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 5 cut(s) 24, 95, 247, 451, 485
HspAI GCGC 1 cut(s) 189
KpnI GGTACC 1 cut(s) 447
Kzo9I GATC 3 cut(s) 8, 210, 756
LmnI GCTCC 4 cut(s) 159, 196, 496, 718
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 1 cut(s) 630
MaeI CTAG 2 cut(s) 281, 290
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 2 cut(s) 334, 529
MalI GATC 3 cut(s) 10, 212, 758
MboI GATC 3 cut(s) 8, 210, 756
MfeI CAATTG 1 cut(s) 425
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 6 cut(s) 309, 425, 459, 498, 687, 730
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 7 cut(s) 95, 358, 364, 582, 611, 682, 784
MseI TTAA 5 cut(s) 378, 497, 510, 690, 705
MslI CAYNNNNRTG 2 cut(s) 199, 713
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 208, 255
MunI CAATTG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 4 cut(s) 259, 482, 550, 640
NciI CCSGG 1 cut(s) 208
NcoI CCATGG 1 cut(s) 447
NdeII GATC 3 cut(s) 8, 210, 756
NlaIII CATG 5 cut(s) 24, 95, 247, 451, 485
NlaIV GGNNCC 2 cut(s) 10, 445
NspI RCATGY 1 cut(s) 24
PagI TCATGA 1 cut(s) 91
PciI ACATGT 1 cut(s) 20
PfeI GAWTC 2 cut(s) 182, 395
PflFI GACNNNGTC 1 cut(s) 291
PflMI CCANNNNNTGG 2 cut(s) 185, 500
PkrI GCNGC 1 cut(s) 436
PscI ACATGT 1 cut(s) 20
PshBI ATTAAT 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 253
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 10, 445
PsrI GAACNNNNNNTAC 2 cut(s) 462, 494
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 3 cut(s) 185, 254, 473
PsuI RGATCY 1 cut(s) 8
PsyI GACNNNGTC 1 cut(s) 291
RsaI GTAC 2 cut(s) 349, 445
RsaNI GTAC 2 cut(s) 348, 444
RseI CAYNNNNRTG 2 cut(s) 199, 713
SaqAI TTAA 5 cut(s) 378, 497, 510, 690, 705
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 3 cut(s) 8, 210, 756
ScaI AGTACT 1 cut(s) 349
ScrFI CCNGG 2 cut(s) 208, 255
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 1 cut(s) 630
SfcI CTRYAG 1 cut(s) 474
SmiMI CAYNNNNRTG 2 cut(s) 199, 713
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 6 cut(s) 309, 425, 459, 498, 687, 730
SsiI CCGC 1 cut(s) 157
SspI AATATT 1 cut(s) 667
SspMI CTAG 2 cut(s) 281, 290
StyD4I CCNGG 2 cut(s) 206, 253
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 4 cut(s) 217, 340, 469, 535
TaiI ACGT 1 cut(s) 615
TaqI TCGA 2 cut(s) 681, 759
TasI AATT 6 cut(s) 309, 425, 459, 498, 687, 730
TatI WGTACW 1 cut(s) 347
TfiI GAWTC 2 cut(s) 182, 395
Tru1I TTAA 5 cut(s) 378, 497, 510, 690, 705
Tru9I TTAA 5 cut(s) 378, 497, 510, 690, 705
TscAI CASTG 2 cut(s) 220, 561
TseI GCWGC 1 cut(s) 434
TspDTI ATGAA 2 cut(s) 245, 470
TspRI CASTG 2 cut(s) 220, 561
Tth111I GACNNNGTC 1 cut(s) 291
Van91I CCANNNNNTGG 2 cut(s) 185, 500
Vha464I CTTAAG 1 cut(s) 509
VspI ATTAAT 1 cut(s) 497
XceI RCATGY 1 cut(s) 24
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 2 cut(s) 281, 290
ZrmI AGTACT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.