Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Reverse (-)

15942410 .. 15946034

Gene Structure 3625 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g24090.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1731 bp

ATGACCACCCATCATAGAAAACCCATAAAATCCAGCTTCCCCTCACTCTCGAAGCGATGTTCCTCCCTCTCCAAAACCTCCAAATTCATCCTCATCTCCATGGCCTCCCTCGGCTTCGTCTCCCTCCTCACCCTCACCCTCTTCCTCTCCCTCTCCTCCGCCGCCCGCCACCACCACCCTAAGCCCTCCGCCACCAAATCAGCCGCAACCCAGATCCAACTCGCCTGCAAAGCCACCCGCTTCCCCGACACGTGTCAATCCTCCCTCTCCCAGCTCCCTGACCTCCCTCCAAACCCCACCCCCGTCGAGACCATCGCTCTCGCCGTCCGTGTCTCCTCCGATGGGCTCCACACGGCCCAGAAAATGGTCACCGACATTCTCGGAGCTTCGAAAGGAAACCAGAACCGCACCACCGCCGCGAACAACTGCCTCGACGTGCTTAAGAATTCCGAGTACCGCGTTTCTCGCGCCACCGAGGAGATGTCACGTGCGCGGATTAAGAGCGCACGTGCCGCGTTCAGCGCCGCGCTGCTGTACCAGTACGACTGCTGGTCGGCGCTCAAGTACGCCAACGACACCCAGATGGTGAACCAGACGATGTCGTTTCTCGACGGGCTGATCGGACGGTCCAGCAACGCCCTCAGCATGATGTTCTCCTACGACAACTTCGGCAACGACACCTCCCTGTGGGCCCCGCCCAGGACGGAGCGCGACGGGTTCTGGGAGCGGACCGGCGGGTCGGGCGACTTTTCCGGCGGCGGAGGAGGAGGAGTGCCGAAGGGTCTGAAGGCGGACGTGACGGTGTGTAAGGAGGAGGGGTGTGAGTGCCGGACGGTGCAGGAGGCGGTGGATAAGGTGCCGGAGAATTTGGGAGCAGGGAAGAGGTTTGTGATTCAGATAAAGACTGGGGTGTACGAAGAGACGGTGAGGGTGGGCTTAGCGAAGCGGAACCTGGTGTTCCTCGGCGACGGGATGGGCAAGACGGTAATTACCGGGTCGTTGAATGTGGGCCAGCCCGGAGTCACCACTTACAATTCCGCCACCGTCGGGGTTCTTGGTGATGGGTTCATGGCCAGTGGTCTTACAATTTCGAACACAGCAGGCCCTGACGCCCACCAGGCAGTGGCTTTCCGATCAGACAGTGATCTATCTGTAATTGAGAACTGTGAATTCTTAGGAAATCAGGACACTTTATACGCACATGGAAACCGTCAGCTCTATAAATCGTGCAAAATTCAAGGCAATGTGGATTTCATCTTCGGAAACTCAGCTGCCATTTTCCAGGACTGCAACATCCTTGTTGCTCCCCGACAACTCAAACCCGAGAAGGGTGAAAACAATGCCGTCACAGCCCATGGTAGGACAGACCCTGCACAGTCAACAGGTTTCGTGTTCCAAAATTGCTTGATCAACGGAACTGAAGAGTACATGAAGTTGTATTACAGCAAGCCCCAAGTGCACAAAAACTACTTGGGGAGGCCGTGGAAGGAGTATTCAAGGACGGTTTTCTTAAACTGCAGTATGGAAGCTCTGATTACCCGGCAGGGTTGGATGCCTTGGAGTGGTGATTTTGCTTTAAAGACACTTTTCTACGGGGAGTACGGGAATACCGGAACAGGTTCAGATCTCACCCAGAGAGTGACATGGAGCAGCAAAATTCCAACCGAGCATGTTAATACATATTCAGTGCAGAATTTTATTCAGGGAGACGAGTGGATGGAGACCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

577

Amino Acids

62.86

Weight (kDa)

8.65

Isoelectric Point (pI)

31.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	71 - 216	3.2e-21	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	265 - 570	2.9e-118	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	736
AccB1I	GGYRCC	1 cut(s)	856
AccB7I	CCANNNNNTGG	2 cut(s)	364, 1123
AccBSI	CCGCTC	1 cut(s)	727
AccII	CGCG	7 cut(s)	419, 459, 468, 493, 515, 527, 711
AclWI	GGATC	1 cut(s)	208
AcoI	YGGCCR	1 cut(s)	1071
AcsI	RAATTY	7 cut(s)	83, 445, 865, 1169, 1233, 1656, 1693
AcuI	CTGAAG	2 cut(s)	806, 1440
AcvI	CACGTG	3 cut(s)	252, 488, 509
AcyI	GRCGYC	1 cut(s)	1110
AfaI	GTAC	7 cut(s)	455, 536, 542, 566, 914, 1427, 1601
AfiI	CCNNNNNNNGG	9 cut(s)	342, 364, 687, 699, 1047, 1123, 1328, 1359, 1562
AflII	CTTAAG	1 cut(s)	440
AflIII	ACRYGT	2 cut(s)	249, 251
AgsI	TTSAA	3 cut(s)	1003, 1238, 1497
AhdI	GACNNNNNGTC	1 cut(s)	550
AjiI	CACGTC	2 cut(s)	436, 796
AjnI	CCWGG	4 cut(s)	698, 951, 1116, 1281
AluBI	AGCT	6 cut(s)	36, 274, 386, 1216, 1271, 1529
AluI	AGCT	6 cut(s)	36, 274, 386, 1216, 1271, 1529
Alw21I	GWGCWC	1 cut(s)	1461
Alw26I	GTCTC	6 cut(s)	124, 302, 337, 914, 1701, 1715
Alw44I	GTGCAC	1 cut(s)	1457
AlwI	GGATC	1 cut(s)	208
AlwNI	CAGNNNCTG	2 cut(s)	1106, 1370
Ama87I	CYCGRG	1 cut(s)	1322
AoxI	GGCC	7 cut(s)	102, 354, 690, 1009, 1071, 1102, 1478
ApaI	GGGCCC	1 cut(s)	694
ApaLI	GTGCAC	1 cut(s)	1457
ApeKI	GCWGC	3 cut(s)	529, 1271, 1650
ApoI	RAATTY	7 cut(s)	83, 445, 865, 1169, 1233, 1656, 1693
Asp700I	GAANNNNTTC	1 cut(s)	1618
AspLEI	GCGC	7 cut(s)	470, 493, 506, 524, 529, 559, 711
AspS9I	GGNCC	7 cut(s)	355, 627, 690, 691, 729, 1009, 1103
AsuC2I	CCSGG	3 cut(s)	994, 1017, 1540
AsuII	TTCGAA	2 cut(s)	389, 1091
AvaI	CYCGRG	1 cut(s)	1322
AvaII	GGWCC	2 cut(s)	627, 729
BaeGI	GKGCMC	2 cut(s)	694, 1461
BalI	TGGCCA	1 cut(s)	1073
BanI	GGYRCC	1 cut(s)	856
BanII	GRGCYC	2 cut(s)	348, 694
BarI	GAAGNNNNNNTAC	2 cut(s)	1424, 1456
BbrPI	CACGTG	3 cut(s)	252, 488, 509
Bbv12I	GWGCWC	1 cut(s)	1461
BbvCI	CCTCAGC	1 cut(s)	641
BbvI	GCAGC	3 cut(s)	516, 1258, 1662
BccI	CCATC	7 cut(s)	18, 320, 335, 577, 967, 1055, 1711
BceAI	ACGGC	4 cut(s)	308, 369, 1328, 1465
BciT130I	CCWGG	4 cut(s)	700, 953, 1118, 1283
BclI	TGATCA	1 cut(s)	1407
BcnI	CCSGG	3 cut(s)	994, 1017, 1540
BcoDI	GTCTC	6 cut(s)	124, 302, 337, 914, 1701, 1715
BfmI	CTRYAG	1 cut(s)	1516
BfoI	RGCGCY	2 cut(s)	525, 560
BfrI	CTTAAG	1 cut(s)	440
BglI	GCCNNNNNGGC	1 cut(s)	1118
BglII	AGATCT	1 cut(s)	1624
BisI	GCNGC	9 cut(s)	162, 204, 417, 513, 525, 530, 757, 1272, 1651
BlpI	GCTNAGC	1 cut(s)	937
BlsI	GCNGC	9 cut(s)	163, 205, 418, 514, 526, 531, 758, 1273, 1652
Bme1390I	CCNGG	7 cut(s)	700, 953, 994, 1017, 1118, 1283, 1540
Bme18I	GGWCC	2 cut(s)	627, 729
BmeRI	GACNNNNNGTC	1 cut(s)	550
BmeT110I	CYCGRG	1 cut(s)	1322
BmgBI	CACGTC	2 cut(s)	436, 796
BmgT120I	GGNCC	7 cut(s)	355, 627, 690, 691, 729, 1009, 1103
BmiI	GGNNCC	5 cut(s)	347, 692, 693, 858, 950
BmrFI	CCNGG	7 cut(s)	700, 953, 994, 1017, 1118, 1283, 1540
BmrI	ACTGGG	1 cut(s)	915
BmsI	GCATC	1 cut(s)	1542
BmuI	ACTGGG	1 cut(s)	915
BoxI	GACNNNNGTC	1 cut(s)	252
Bpu10I	CCTNAGC	2 cut(s)	180, 641
Bpu1102I	GCTNAGC	1 cut(s)	937
Bpu14I	TTCGAA	2 cut(s)	389, 1091
BpuEI	CTTGAG	1 cut(s)	545
BpuMI	CCSGG	3 cut(s)	994, 1017, 1540
BsaAI	YACGTR	3 cut(s)	252, 488, 509
BsaHI	GRCGYC	1 cut(s)	1110
BsaI	GGTCTC	2 cut(s)	302, 1715
BsaJI	CCNNGG	8 cut(s)	99, 109, 474, 698, 961, 1354, 1481, 1556
BsaWI	WCCGGW	1 cut(s)	1610
BsaXI	ACNNNNNCTCC	6 cut(s)	665, 695, 756, 786, 803, 833
Bsc4I	CCNNNNNNNGG	9 cut(s)	342, 364, 687, 699, 1047, 1123, 1328, 1359, 1562
Bse118I	RCCGGY	1 cut(s)	731
Bse1I	ACTGG	3 cut(s)	538, 910, 1074
Bse3DI	GCAATG	1 cut(s)	1249
BseBI	CCWGG	4 cut(s)	700, 953, 1118, 1283
BseDI	CCNNGG	8 cut(s)	99, 109, 474, 698, 961, 1354, 1481, 1556
BseGI	GGATG	5 cut(s)	87, 978, 1293, 1557, 1722
BseLI	CCNNNNNNNGG	9 cut(s)	342, 364, 687, 699, 1047, 1123, 1328, 1359, 1562
BseMI	GCAATG	1 cut(s)	1249
BseMII	CTCAG	2 cut(s)	655, 1281
BseNI	ACTGG	3 cut(s)	538, 910, 1074
BseRI	GAGGAG	8 cut(s)	116, 145, 325, 491, 777, 780, 783, 827
BseSI	GKGCMC	2 cut(s)	694, 1461
BseXI	GCAGC	3 cut(s)	516, 1258, 1662
BseYI	CCCAGC	1 cut(s)	270
BsgI	GTGCAG	3 cut(s)	857, 1356, 1709
Bsh1236I	CGCG	7 cut(s)	419, 459, 468, 493, 515, 527, 711
BshFI	GGCC	7 cut(s)	104, 356, 692, 1011, 1073, 1104, 1480
BshNI	GGYRCC	1 cut(s)	856
BsiHKAI	GWGCWC	1 cut(s)	1461
BsiHKCI	CYCGRG	1 cut(s)	1322
BsiSI	CCGG	8 cut(s)	732, 753, 829, 860, 993, 1017, 1540, 1611
BslI	CCNNNNNNNGG	9 cut(s)	342, 364, 687, 699, 1047, 1123, 1328, 1359, 1562
BsmAI	GTCTC	6 cut(s)	124, 302, 337, 914, 1701, 1715
BsmBI	CGTCTC	3 cut(s)	124, 914, 1701
BsnI	GGCC	7 cut(s)	104, 356, 692, 1011, 1073, 1104, 1480
Bso31I	GGTCTC	2 cut(s)	302, 1715
BsoBI	CYCGRG	1 cut(s)	1322
Bsp119I	TTCGAA	2 cut(s)	389, 1091
Bsp120I	GGGCCC	1 cut(s)	690
Bsp1286I	GDGCHC	3 cut(s)	348, 694, 1461
Bsp143I	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
Bsp1720I	GCTNAGC	1 cut(s)	937
Bsp19I	CCATGG	2 cut(s)	99, 1354
BspANI	GGCC	7 cut(s)	104, 356, 692, 1011, 1073, 1104, 1480
BspCNI	CTCAG	2 cut(s)	654, 1280
BspFNI	CGCG	7 cut(s)	419, 459, 468, 493, 515, 527, 711
BspLI	GGNNCC	5 cut(s)	347, 692, 693, 858, 950
BspMAI	CTGCAG	1 cut(s)	1520
BspPI	GGATC	1 cut(s)	208
BspT104I	TTCGAA	2 cut(s)	389, 1091
BspT107I	GGYRCC	1 cut(s)	856
BspTI	CTTAAG	1 cut(s)	440
BspTNI	GGTCTC	2 cut(s)	302, 1715
BsrBI	CCGCTC	1 cut(s)	727
BsrDI	GCAATG	1 cut(s)	1249
BsrFI	RCCGGY	1 cut(s)	731
BsrI	ACTGG	3 cut(s)	538, 910, 1074
BssAI	RCCGGY	1 cut(s)	731
BssECI	CCNNGG	8 cut(s)	99, 109, 474, 698, 961, 1354, 1481, 1556
BssMI	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
BssNI	GRCGYC	1 cut(s)	1110
BssT1I	CCWWGG	3 cut(s)	99, 1354, 1556
Bst2UI	CCWGG	4 cut(s)	700, 953, 1118, 1283
Bst6I	CTCTTC	4 cut(s)	146, 875, 912, 1416
BstACI	GRCGYC	1 cut(s)	1110
BstAFI	CTTAAG	1 cut(s)	440
BstBAI	YACGTR	3 cut(s)	252, 488, 509
BstBI	TTCGAA	2 cut(s)	389, 1091
BstC8I	GCNNGC	5 cut(s)	166, 226, 1013, 1102, 1448
BstDEI	CTNAG	5 cut(s)	180, 641, 937, 1174, 1267
BstDSI	CCRYGG	3 cut(s)	99, 1354, 1481
BstEII	GGTNACC	1 cut(s)	367
BstF5I	GGATG	5 cut(s)	87, 978, 1293, 1557, 1722
BstFNI	CGCG	7 cut(s)	419, 459, 468, 493, 515, 527, 711
BstH2I	RGCGCY	2 cut(s)	525, 560
BstHHI	GCGC	7 cut(s)	470, 493, 506, 524, 529, 559, 711
BstKTI	GATC	6 cut(s)	216, 621, 1136, 1147, 1410, 1627
BstMAI	GTCTC	6 cut(s)	124, 302, 337, 914, 1701, 1715
BstMBI	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
BstMWI	GCNNNNNNNGC	8 cut(s)	230, 465, 512, 521, 741, 1118, 1349, 1456
BstNI	CCWGG	4 cut(s)	700, 953, 1118, 1283
BstNSI	RCATGY	1 cut(s)	1673
BstPAI	GACNNNNGTC	1 cut(s)	252
BstPI	GGTNACC	1 cut(s)	367
BstSCI	CCNGG	7 cut(s)	698, 951, 992, 1015, 1116, 1281, 1538
BstSFI	CTRYAG	1 cut(s)	1516
BstSLI	GKGCMC	2 cut(s)	694, 1461
BstUI	CGCG	7 cut(s)	419, 459, 468, 493, 515, 527, 711
BstV1I	GCAGC	3 cut(s)	516, 1258, 1662
BstX2I	RGATCY	2 cut(s)	213, 1624
BstYI	RGATCY	2 cut(s)	213, 1624
BsuRI	GGCC	7 cut(s)	104, 356, 692, 1011, 1073, 1104, 1480
BtgI	CCRYGG	3 cut(s)	99, 1354, 1481
BtgZI	GCGATG	2 cut(s)	70, 298
BtrI	CACGTC	2 cut(s)	436, 796
BtsCI	GGATG	5 cut(s)	87, 978, 1293, 1557, 1722
BtsI	GCAGTG	1 cut(s)	1128
BtsIMutI	CAGTG	4 cut(s)	1081, 1128, 1147, 1692
Cac8I	GCNNGC	5 cut(s)	166, 226, 1013, 1102, 1448
CaiI	CAGNNNCTG	2 cut(s)	1106, 1370
CfoI	GCGC	7 cut(s)	470, 493, 506, 524, 529, 559, 711
Cfr10I	RCCGGY	1 cut(s)	731
Cfr13I	GGNCC	7 cut(s)	355, 627, 690, 691, 729, 1009, 1103
CpoI	CGGWCCG	1 cut(s)	729
CseI	GACGC	1 cut(s)	1118
CsiI	ACCWGGT	1 cut(s)	951
Csp6I	GTAC	7 cut(s)	454, 535, 541, 565, 913, 1426, 1600
CspI	CGGWCCG	1 cut(s)	729
CviAII	CATG	8 cut(s)	100, 646, 1069, 1202, 1355, 1429, 1644, 1670
CviQI	GTAC	7 cut(s)	454, 535, 541, 565, 913, 1426, 1600
DdeI	CTNAG	5 cut(s)	180, 641, 937, 1174, 1267
DpnI	GATC	6 cut(s)	215, 620, 1135, 1146, 1409, 1626
DpnII	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
DraI	TTTAAA	1 cut(s)	1578
DrdI	GACNNNNNNGTC	1 cut(s)	736
DriI	GACNNNNNGTC	1 cut(s)	550
DseDI	GACNNNNNNGTC	1 cut(s)	736
EaeI	YGGCCR	1 cut(s)	1071
Eam1104I	CTCTTC	4 cut(s)	146, 875, 912, 1416
Eam1105I	GACNNNNNGTC	1 cut(s)	550
EarI	CTCTTC	4 cut(s)	146, 875, 912, 1416
EciI	GGCGGA	5 cut(s)	148, 178, 774, 806, 1027
Eco130I	CCWWGG	3 cut(s)	99, 1354, 1556
Eco24I	GRGCYC	2 cut(s)	348, 694
Eco31I	GGTCTC	2 cut(s)	302, 1715
Eco47I	GGWCC	2 cut(s)	627, 729
Eco57I	CTGAAG	2 cut(s)	806, 1440
Eco72I	CACGTG	3 cut(s)	252, 488, 509
Eco88I	CYCGRG	1 cut(s)	1322
Eco91I	GGTNACC	1 cut(s)	367
EcoO109I	RGGNCCY	2 cut(s)	691, 1103
EcoO65I	GGTNACC	1 cut(s)	367
EcoRI	GAATTC	2 cut(s)	445, 1169
EcoRII	CCWGG	4 cut(s)	698, 951, 1116, 1281
EcoT14I	CCWWGG	3 cut(s)	99, 1354, 1556
EcoT38I	GRGCYC	2 cut(s)	348, 694
ErhI	CCWWGG	3 cut(s)	99, 1354, 1556
Esp3I	CGTCTC	3 cut(s)	124, 914, 1701
FaeI	CATG	8 cut(s)	103, 649, 1072, 1205, 1358, 1432, 1647, 1673
FatI	CATG	8 cut(s)	99, 645, 1068, 1201, 1354, 1428, 1643, 1669
FauI	CCCGC	4 cut(s)	173, 245, 702, 728
FbaI	TGATCA	1 cut(s)	1407
Fnu4HI	GCNGC	9 cut(s)	162, 204, 417, 513, 525, 530, 757, 1272, 1651
FokI	GGATG	4 cut(s)	74, 985, 1280, 1564
FriOI	GRGCYC	2 cut(s)	348, 694
Fsp4HI	GCNGC	9 cut(s)	162, 204, 417, 513, 525, 530, 757, 1272, 1651
GlaI	GCGC	7 cut(s)	469, 492, 505, 523, 528, 558, 710
GluI	GCNGC	9 cut(s)	162, 204, 417, 513, 525, 530, 757, 1272, 1651
GsaI	CCCAGC	1 cut(s)	274
HaeII	RGCGCY	2 cut(s)	525, 560
HaeIII	GGCC	7 cut(s)	104, 356, 692, 1011, 1073, 1104, 1480
HapII	CCGG	8 cut(s)	732, 753, 829, 860, 993, 1017, 1540, 1611
HgaI	GACGC	1 cut(s)	1118
HhaI	GCGC	7 cut(s)	470, 493, 506, 524, 529, 559, 711
Hin1I	GRCGYC	1 cut(s)	1110
Hin1II	CATG	8 cut(s)	103, 649, 1072, 1205, 1358, 1432, 1647, 1673
Hin6I	GCGC	7 cut(s)	468, 491, 504, 522, 527, 557, 709
HinP1I	GCGC	7 cut(s)	468, 491, 504, 522, 527, 557, 709
HincII	GTYRAC	1 cut(s)	1380
HindII	GTYRAC	1 cut(s)	1380
HinfI	GANTC	2 cut(s)	892, 1020
HpaII	CCGG	8 cut(s)	732, 753, 829, 860, 993, 1017, 1540, 1611
Hpy166II	GTNNAC	4 cut(s)	589, 913, 1380, 1459
Hpy188III	TCNNGA	4 cut(s)	49, 307, 608, 1184
Hpy8I	GTNNAC	4 cut(s)	589, 913, 1380, 1459
Hpy99I	CGWCG	6 cut(s)	308, 437, 614, 716, 971, 1049
HpyAV	CCTTC	4 cut(s)	772, 781, 1321, 1480
HpyCH4IV	ACGT	5 cut(s)	251, 435, 487, 508, 795
HpyCH4V	TGCA	8 cut(s)	228, 838, 1230, 1290, 1373, 1459, 1518, 1690
HpyF10VI	GCNNNNNNNGC	8 cut(s)	230, 465, 512, 521, 741, 1118, 1349, 1456
HpyF3I	CTNAG	5 cut(s)	180, 641, 937, 1174, 1267
HpySE526I	ACGT	5 cut(s)	251, 435, 487, 508, 795
Hsp92I	GRCGYC	1 cut(s)	1110
Hsp92II	CATG	8 cut(s)	103, 649, 1072, 1205, 1358, 1432, 1647, 1673
HspAI	GCGC	7 cut(s)	468, 491, 504, 522, 527, 557, 709
Ksp22I	TGATCA	1 cut(s)	1407
Kzo9I	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
LmnI	GCTCC	8 cut(s)	279, 351, 383, 706, 724, 872, 1309, 1647
Lsp1109I	GCAGC	3 cut(s)	516, 1258, 1662
LweI	GCATC	1 cut(s)	1542
MabI	ACCWGGT	1 cut(s)	951
MaeII	ACGT	5 cut(s)	251, 435, 487, 508, 795
MaeIII	GTNAC	6 cut(s)	367, 483, 796, 1021, 1345, 1639
MalI	GATC	6 cut(s)	215, 620, 1135, 1146, 1409, 1626
MbiI	CCGCTC	1 cut(s)	727
MboI	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
MboII	GAAGA	5 cut(s)	133, 892, 929, 1249, 1433
MflI	RGATCY	2 cut(s)	213, 1624
MhlI	GDGCHC	3 cut(s)	348, 694, 1461
MlsI	TGGCCA	1 cut(s)	1073
MluNI	TGGCCA	1 cut(s)	1073
MlyI	GAGTC	1 cut(s)	1029
MmeI	TCCRAC	3 cut(s)	241, 1529, 1685
Mox20I	TGGCCA	1 cut(s)	1073
MroXI	GAANNNNTTC	1 cut(s)	1618
MscI	TGGCCA	1 cut(s)	1073
MseI	TTAA	5 cut(s)	441, 498, 1511, 1577, 1674
MslI	CAYNNNNRTG	2 cut(s)	98, 581
Msp20I	TGGCCA	1 cut(s)	1073
MspA1I	CMGCKG	1 cut(s)	1271
MspCI	CTTAAG	1 cut(s)	440
MspI	CCGG	8 cut(s)	732, 753, 829, 860, 993, 1017, 1540, 1611
MspR9I	CCNGG	7 cut(s)	700, 953, 994, 1017, 1118, 1283, 1540
MteI	GCGCNGCGC	1 cut(s)	525
MvaI	CCWGG	4 cut(s)	700, 953, 1118, 1283
MvnI	CGCG	7 cut(s)	419, 459, 468, 493, 515, 527, 711
MwoI	GCNNNNNNNGC	8 cut(s)	230, 465, 512, 521, 741, 1118, 1349, 1456
NciI	CCSGG	3 cut(s)	994, 1017, 1540
NcoI	CCATGG	2 cut(s)	99, 1354
NdeII	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
NlaIII	CATG	8 cut(s)	103, 649, 1072, 1205, 1358, 1432, 1647, 1673
NlaIV	GGNNCC	5 cut(s)	347, 692, 693, 858, 950
NmeAIII	GCCGAG	2 cut(s)	90, 942
NmuCI	GTSAC	6 cut(s)	367, 483, 796, 1021, 1345, 1639
NspI	RCATGY	1 cut(s)	1673
NspV	TTCGAA	2 cut(s)	389, 1091
PcsI	WCGNNNNNNNCGW	1 cut(s)	321
PdmI	GAANNNNTTC	1 cut(s)	1618
PfeI	GAWTC	1 cut(s)	892
PflFI	GACNNNGTC	1 cut(s)	598
PflMI	CCANNNNNTGG	2 cut(s)	364, 1123
PfoI	TCCNGGA	1 cut(s)	1281
PkrI	GCNGC	9 cut(s)	163, 205, 418, 514, 526, 531, 758, 1273, 1652
PleI	GAGTC	1 cut(s)	1028
PmaCI	CACGTG	3 cut(s)	252, 488, 509
PmlI	CACGTG	3 cut(s)	252, 488, 509
PpsI	GAGTC	1 cut(s)	1028
Ppu21I	YACGTR	3 cut(s)	252, 488, 509
PshAI	GACNNNNGTC	1 cut(s)	252
Psp6I	CCWGG	4 cut(s)	698, 951, 1116, 1281
PspCI	CACGTG	3 cut(s)	252, 488, 509
PspEI	GGTNACC	1 cut(s)	367
PspFI	CCCAGC	1 cut(s)	270
PspGI	CCWGG	4 cut(s)	698, 951, 1116, 1281
PspN4I	GGNNCC	5 cut(s)	347, 692, 693, 858, 950
PspOMI	GGGCCC	1 cut(s)	690
PspPI	GGNCC	7 cut(s)	355, 627, 690, 691, 729, 1009, 1103
PstI	CTGCAG	1 cut(s)	1520
PstNI	CAGNNNCTG	2 cut(s)	1106, 1370
PsuI	RGATCY	2 cut(s)	213, 1624
PsyI	GACNNNGTC	1 cut(s)	598
PvuII	CAGCTG	1 cut(s)	1271
RsaI	GTAC	7 cut(s)	455, 536, 542, 566, 914, 1427, 1601
RsaNI	GTAC	7 cut(s)	454, 535, 541, 565, 913, 1426, 1600
RseI	CAYNNNNRTG	2 cut(s)	98, 581
Rsr2I	CGGWCCG	1 cut(s)	729
RsrII	CGGWCCG	1 cut(s)	729
SaqAI	TTAA	5 cut(s)	441, 498, 1511, 1577, 1674
SatI	GCNGC	9 cut(s)	162, 204, 417, 513, 525, 530, 757, 1272, 1651
Sau3AI	GATC	6 cut(s)	213, 618, 1133, 1144, 1407, 1624
Sau96I	GGNCC	7 cut(s)	355, 627, 690, 691, 729, 1009, 1103
SchI	GAGTC	1 cut(s)	1029
ScrFI	CCNGG	7 cut(s)	700, 953, 994, 1017, 1118, 1283, 1540
SduI	GDGCHC	3 cut(s)	348, 694, 1461
SexAI	ACCWGGT	1 cut(s)	951
SfaNI	GCATC	1 cut(s)	1542
SfcI	CTRYAG	1 cut(s)	1516
SfuI	TTCGAA	2 cut(s)	389, 1091
SinI	GGWCC	2 cut(s)	627, 729
SmiMI	CAYNNNNRTG	2 cut(s)	98, 581
SmlI	CTYRAG	2 cut(s)	440, 560
SmoI	CTYRAG	2 cut(s)	440, 560
StyD4I	CCNGG	7 cut(s)	698, 951, 992, 1015, 1116, 1281, 1538
StyI	CCWWGG	3 cut(s)	99, 1354, 1556
TaiI	ACGT	5 cut(s)	254, 438, 490, 511, 798
TaqI	TCGA	6 cut(s)	50, 306, 389, 432, 609, 1091
TatI	WGTACW	1 cut(s)	1425
TauI	GCSGC	6 cut(s)	164, 206, 419, 515, 527, 759
TfiI	GAWTC	1 cut(s)	892
Tru1I	TTAA	5 cut(s)	441, 498, 1511, 1577, 1674
Tru9I	TTAA	5 cut(s)	441, 498, 1511, 1577, 1674
TscAI	CASTG	4 cut(s)	1081, 1128, 1147, 1692
TseFI	GTSAC	6 cut(s)	367, 483, 796, 1021, 1345, 1639
TseI	GCWGC	3 cut(s)	529, 1271, 1650
Tsp45I	GTSAC	6 cut(s)	367, 483, 796, 1021, 1345, 1639
TspDTI	ATGAA	4 cut(s)	76, 1057, 1243, 1445
TspGWI	ACGGA	3 cut(s)	317, 719, 1428
TspRI	CASTG	4 cut(s)	1081, 1128, 1147, 1692
Tth111I	GACNNNGTC	1 cut(s)	598
Van91I	CCANNNNNTGG	2 cut(s)	364, 1123
Vha464I	CTTAAG	1 cut(s)	440
VneI	GTGCAC	1 cut(s)	1457
VpaK11BI	GGWCC	2 cut(s)	627, 729
XapI	RAATTY	7 cut(s)	83, 445, 865, 1169, 1233, 1656, 1693
XceI	RCATGY	1 cut(s)	1673
XmnI	GAANNNNTTC	1 cut(s)	1618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.