Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Forward (+)

3086555 .. 3088192

Gene Structure 1638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG045900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGTCTACCCGCGTCTTTCTCCTCACACTCGCTCTCTGCATCTCTCTTTCCTCCTCCTCCGCCGCCAGCTTCCGCTCCATCCCCAAGCCCACCGCCGACGTATCTCCGATCCAACAAGCCTGCAAATCCACCCGCTTCCCTGACACATGTCGGGCCTCCCTGACAAAAAAAATCCATGACCTTCCCCCAAACCCTACCCCTCTCCAAACACTTCAACTAGCCCTCAATGTCTCCTCCGACGGCCTCAACACCGCCAAAAAGATGGTTTCCGACATTCTCAAAACCGCCGCCGGCAGCCAGAACCGCACAACCGCCGCCAAAAACTGCCTCGATTTTCTACAAAATTCCGAGTACCGTATTTCAATCACCAATGACGGCCTCTCACGTGGCCGTACCAAGACCGCACGAGCCGCCATGAGCTCCGCCCTGATGTACCAGTACGACTGCTGGTCCGCCTTCAAGTACGCCAACGACACCCAGTTGGTCGTCTCCACCATGTCGTTCATTAACGACACGCTGACCGTGGACTCCAGCAACGCCCTTAGCATGATCTTCTCCTACGAAAACTTCGGAAACGACACCAAATCCTGGACCCCGCCCAAGACCGAGCGCGACGGGTTCTGGGAACCTGTCACGCCCGGATCACAAAAGTCCCCTCCAGAACTTTCGATACCGAAAAATCTGACGGCAGACGTGACTGTTTGCAAGGACAAAGGTGTTAAGTGTTACAGGACCGTACAGGAGGCGGTGAACGCGGCGCCGGAAAACTCCGACAAGAAATTCGTGATTCAGATAAATGCCGGGGTGTATGAAGAGATTGTTAAGGTTCCTCTAGGGAAGAGAAATGTTGTGTTTTTAGGGCAAGGGATGGGCAAAACAGTAATTACAGGTTCTTTGTTTGTGGGCCAGCCGGGAATTTCGACCTACAACTCCGCCACCGTGGGTGTTTACGGCGACGGCTTCATGGCCAGAGGCGTCACATTCCAAAACACGGCGGGCCCCGACGCCCAGCAGGAGGTTCACCAGGCGGTGGCTTTCCGATCAGACAGTGATCACTCTATCATAGAAAATTGTGAGTTCCTAGGGCACCAAGACACTCTCTACGCCCACGGCAACCGCCAGTTCTACAAGTCATGCAAAATTCAAGGCAATGTAGATTTTATATTTGGAAACTCGGCTGCCATTTTCGACGGCTGCACCATCCTCATTGCGCCGAGGCAAGTAAACCCCGAAAAAGGCGAGAAGAATGCCGTGACGGCTCACGGCAGGACTGACCCGGCGCAGGCGACAGGGTTCGTGTTCCGGAACTGCTTCATAAACGGAACGGAAGAGTACATGAAGTTGTACAACATTAACCCCAAAGTGCACTTGAACTTCTTGGGGAGGCCGTGGAAGGAGTTTTCGAGGACGGTGTTCTTGAACTGTAGTATGGAAGCTCTGGTTTCGAAAGAAGGGTGGTTGCCTTGGAGTGAGGAGTTTGCTTTGGCGACGCTTTACTATGGGGAGTATGGGAACTCGGGACCGGGGGCGGATTTGGCCGAGAGGGTGAAGTGGAGTAGCCGGATTCCGGTAGACCATGTGGGTGCATATTCGGTGCAAAATTTTATTCAGGGAGATGAGTGGTTGGTTTCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

545

Amino Acids

59.86

Weight (kDa)

7.89

Isoelectric Point (pI)

31.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	37 - 183	5.3e-20	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	231 - 539	1.1e-112	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	757, 1086
AccB7I	CCANNNNNTGG	2 cut(s)	588, 1030
AccBSI	CCGCTC	1 cut(s)	75
AccI	GTMKAC	2 cut(s)	5, 1572
AccII	CGCG	3 cut(s)	12, 612, 755
AccIII	TCCGGA	1 cut(s)	1302
AclWI	GGATC	2 cut(s)	103, 649
AcoI	YGGCCR	3 cut(s)	388, 966, 1536
AcsI	RAATTY	5 cut(s)	343, 779, 915, 1140, 1600
AcvI	CACGTG	1 cut(s)	386
AcyI	GRCGYC	3 cut(s)	758, 975, 1005
AfaI	GTAC	8 cut(s)	353, 394, 434, 440, 464, 738, 1334, 1346
AfiI	CCNNNNNNNGG	7 cut(s)	588, 991, 1015, 1030, 1235, 1282, 1567
AflIII	ACRYGT	1 cut(s)	146
AgsI	TTSAA	6 cut(s)	215, 363, 460, 1145, 1372, 1420
AhdI	GACNNNNNGTC	1 cut(s)	448
AjiI	CACGTC	1 cut(s)	694
AjnI	CCWGG	2 cut(s)	587, 1023
AluBI	AGCT	3 cut(s)	69, 420, 1436
AluI	AGCT	3 cut(s)	69, 420, 1436
Alw21I	GWGCWC	2 cut(s)	422, 1368
Alw26I	GTCTC	2 cut(s)	235, 493
Alw44I	GTGCAC	1 cut(s)	1364
AlwI	GGATC	2 cut(s)	103, 649
Ama87I	CYCGRG	1 cut(s)	1516
Aor13HI	TCCGGA	1 cut(s)	1302
AoxI	GGCC	9 cut(s)	153, 241, 376, 388, 904, 966, 997, 1385, 1536
ApaI	GGGCCC	1 cut(s)	1001
ApaLI	GTGCAC	1 cut(s)	1364
ApeKI	GCWGC	3 cut(s)	294, 1178, 1194
ApoI	RAATTY	5 cut(s)	343, 779, 915, 1140, 1600
ArsI	GACNNNNNNTTYG	2 cut(s)	764, 796
Asp700I	GAANNNNTTC	1 cut(s)	1310
AspA2I	CCTAGG	1 cut(s)	1081
AspLEI	GCGC	4 cut(s)	612, 760, 1213, 1282
AspS9I	GGNCC	8 cut(s)	153, 450, 591, 732, 904, 997, 998, 1520
AsuC2I	CCSGG	5 cut(s)	639, 802, 912, 1277, 1524
AsuHPI	GGTGA	4 cut(s)	358, 760, 1013, 1558
AsuII	TTCGAA	1 cut(s)	1445
AvaI	CYCGRG	1 cut(s)	1516
AvaII	GGWCC	4 cut(s)	450, 591, 732, 1520
AvrII	CCTAGG	1 cut(s)	1081
BaeGI	GKGCMC	3 cut(s)	1001, 1089, 1368
BalI	TGGCCA	1 cut(s)	968
BanI	GGYRCC	2 cut(s)	757, 1086
BanII	GRGCYC	2 cut(s)	422, 1001
BauI	CACGAG	1 cut(s)	405
BbrPI	CACGTG	1 cut(s)	386
Bbv12I	GWGCWC	2 cut(s)	422, 1368
BbvI	GCAGC	3 cut(s)	306, 1165, 1181
BccI	CCATC	4 cut(s)	86, 256, 862, 1208
BcgI	CGANNNNNNTGC	2 cut(s)	1229, 1263
BciT130I	CCWGG	2 cut(s)	589, 1025
BclI	TGATCA	1 cut(s)	1051
BcnI	CCSGG	5 cut(s)	639, 802, 912, 1277, 1524
BcoDI	GTCTC	2 cut(s)	235, 493
BfaI	CTAG	4 cut(s)	218, 833, 1082, 1636
BfmI	CTRYAG	1 cut(s)	1423
BfoI	RGCGCY	1 cut(s)	761
BglI	GCCNNNNNGGC	1 cut(s)	1256
BisI	GCNGC	8 cut(s)	63, 288, 295, 315, 411, 756, 1179, 1195
BlnI	CCTAGG	1 cut(s)	1081
BlsI	GCNGC	8 cut(s)	64, 289, 296, 316, 412, 757, 1180, 1196
Bme1390I	CCNGG	7 cut(s)	589, 639, 802, 912, 1025, 1277, 1524
Bme18I	GGWCC	4 cut(s)	450, 591, 732, 1520
BmeRI	GACNNNNNGTC	1 cut(s)	448
BmeT110I	CYCGRG	1 cut(s)	1516
BmgBI	CACGTC	1 cut(s)	694
BmgT120I	GGNCC	8 cut(s)	153, 450, 591, 732, 904, 997, 998, 1520
BmiI	GGNNCC	8 cut(s)	593, 627, 759, 828, 999, 1000, 1088, 1521
BmrFI	CCNGG	7 cut(s)	589, 639, 802, 912, 1025, 1277, 1524
BmrI	ACTGGG	1 cut(s)	472
BmsI	GCATC	1 cut(s)	48
BmuI	ACTGGG	1 cut(s)	472
BoxI	GACNNNNGTC	1 cut(s)	147
BpmI	CTGGAG	2 cut(s)	514, 642
Bpu10I	CCTNAGC	1 cut(s)	542
Bpu14I	TTCGAA	1 cut(s)	1445
BpuMI	CCSGG	5 cut(s)	639, 802, 912, 1277, 1524
BsaAI	YACGTR	1 cut(s)	386
BsaHI	GRCGYC	3 cut(s)	758, 975, 1005
BsaJI	CCNNGG	9 cut(s)	522, 801, 939, 1081, 1108, 1214, 1388, 1463, 1523
BsaWI	WCCGGW	2 cut(s)	1302, 1567
Bsc4I	CCNNNNNNNGG	7 cut(s)	588, 991, 1015, 1030, 1235, 1282, 1567
Bse118I	RCCGGY	1 cut(s)	290
Bse1I	ACTGG	3 cut(s)	436, 478, 1120
Bse3DI	GCAATG	2 cut(s)	1156, 1206
BseAI	TCCGGA	1 cut(s)	1302
BseBI	CCWGG	2 cut(s)	589, 1025
BseDI	CCNNGG	9 cut(s)	522, 801, 939, 1081, 1108, 1214, 1388, 1463, 1523
BseGI	GGATG	3 cut(s)	78, 873, 1200
BseLI	CCNNNNNNNGG	7 cut(s)	588, 991, 1015, 1030, 1235, 1282, 1567
BseMI	GCAATG	2 cut(s)	1156, 1206
BseNI	ACTGG	3 cut(s)	436, 478, 1120
BseRI	GAGGAG	5 cut(s)	11, 43, 46, 223, 1487
BseSI	GKGCMC	3 cut(s)	1001, 1089, 1368
BseXI	GCAGC	3 cut(s)	306, 1165, 1181
BseYI	CCCAGC	1 cut(s)	1008
BsgI	GTGCAG	1 cut(s)	1180
Bsh1236I	CGCG	3 cut(s)	12, 612, 755
BshFI	GGCC	9 cut(s)	155, 243, 378, 390, 906, 968, 999, 1387, 1538
BshNI	GGYRCC	2 cut(s)	757, 1086
BsiHKAI	GWGCWC	2 cut(s)	422, 1368
BsiHKCI	CYCGRG	1 cut(s)	1516
BslFI	GGGAC	2 cut(s)	637, 1533
BslI	CCNNNNNNNGG	7 cut(s)	588, 991, 1015, 1030, 1235, 1282, 1567
BsmAI	GTCTC	2 cut(s)	235, 493
BsmBI	CGTCTC	1 cut(s)	493
BsmFI	GGGAC	2 cut(s)	637, 1533
BsmI	GAATGC	1 cut(s)	1252
BsnI	GGCC	9 cut(s)	155, 243, 378, 390, 906, 968, 999, 1387, 1538
BsoBI	CYCGRG	1 cut(s)	1516
Bsp119I	TTCGAA	1 cut(s)	1445
Bsp120I	GGGCCC	1 cut(s)	997
Bsp1286I	GDGCHC	4 cut(s)	422, 1001, 1089, 1368
Bsp13I	TCCGGA	1 cut(s)	1302
Bsp1407I	TGTACA	1 cut(s)	1344
Bsp143I	GATC	5 cut(s)	108, 549, 641, 1040, 1051
BspANI	GGCC	9 cut(s)	155, 243, 378, 390, 906, 968, 999, 1387, 1538
BspEI	TCCGGA	1 cut(s)	1302
BspFNI	CGCG	3 cut(s)	12, 612, 755
BspLI	GGNNCC	8 cut(s)	593, 627, 759, 828, 999, 1000, 1088, 1521
BspPI	GGATC	2 cut(s)	103, 649
BspT104I	TTCGAA	1 cut(s)	1445
BspT107I	GGYRCC	2 cut(s)	757, 1086
BsrBI	CCGCTC	1 cut(s)	75
BsrDI	GCAATG	2 cut(s)	1156, 1206
BsrFI	RCCGGY	1 cut(s)	290
BsrGI	TGTACA	1 cut(s)	1344
BsrI	ACTGG	3 cut(s)	436, 478, 1120
BssAI	RCCGGY	1 cut(s)	290
BssECI	CCNNGG	9 cut(s)	522, 801, 939, 1081, 1108, 1214, 1388, 1463, 1523
BssMI	GATC	5 cut(s)	108, 549, 641, 1040, 1051
BssNI	GRCGYC	3 cut(s)	758, 975, 1005
BssSI	CACGAG	1 cut(s)	405
BssT1I	CCWWGG	2 cut(s)	1081, 1463
Bst2BI	CACGAG	1 cut(s)	405
Bst2UI	CCWGG	2 cut(s)	589, 1025
Bst4CI	ACNGT	9 cut(s)	356, 523, 700, 736, 880, 940, 1049, 1411, 1424
Bst6I	CTCTTC	3 cut(s)	807, 833, 1323
BstACI	GRCGYC	3 cut(s)	758, 975, 1005
BstAUI	TGTACA	1 cut(s)	1344
BstBAI	YACGTR	1 cut(s)	386
BstBI	TTCGAA	1 cut(s)	1445
BstC8I	GCNNGC	6 cut(s)	67, 121, 292, 908, 997, 1284
BstDEI	CTNAG	1 cut(s)	542
BstDSI	CCRYGG	4 cut(s)	522, 939, 1108, 1388
BstF5I	GGATG	3 cut(s)	78, 873, 1200
BstFNI	CGCG	3 cut(s)	12, 612, 755
BstH2I	RGCGCY	1 cut(s)	761
BstHHI	GCGC	4 cut(s)	612, 760, 1213, 1282
BstKTI	GATC	5 cut(s)	111, 552, 644, 1043, 1054
BstMAI	GTCTC	2 cut(s)	235, 493
BstMBI	GATC	5 cut(s)	108, 549, 641, 1040, 1051
BstMWI	GCNNNNNNNGC	5 cut(s)	303, 410, 752, 1256, 1535
BstNI	CCWGG	2 cut(s)	589, 1025
BstNSI	RCATGY	1 cut(s)	150
BstPAI	GACNNNNGTC	1 cut(s)	147
BstSCI	CCNGG	7 cut(s)	587, 637, 800, 910, 1023, 1275, 1522
BstSFI	CTRYAG	1 cut(s)	1423
BstSLI	GKGCMC	3 cut(s)	1001, 1089, 1368
BstUI	CGCG	3 cut(s)	12, 612, 755
BstV1I	GCAGC	3 cut(s)	306, 1165, 1181
BstXI	CCANNNNNNTGG	1 cut(s)	262
BsuRI	GGCC	9 cut(s)	155, 243, 378, 390, 906, 968, 999, 1387, 1538
BtgI	CCRYGG	4 cut(s)	522, 939, 1108, 1388
BtrI	CACGTC	1 cut(s)	694
BtsCI	GGATG	3 cut(s)	78, 873, 1200
BtsIMutI	CAGTG	1 cut(s)	1054
Cac8I	GCNNGC	6 cut(s)	67, 121, 292, 908, 997, 1284
CfoI	GCGC	4 cut(s)	612, 760, 1213, 1282
Cfr10I	RCCGGY	1 cut(s)	290
Cfr13I	GGNCC	8 cut(s)	153, 450, 591, 732, 904, 997, 998, 1520
CseI	GACGC	3 cut(s)	964, 1013, 1498
Csp6I	GTAC	8 cut(s)	352, 393, 433, 439, 463, 737, 1333, 1345
CviAII	CATG	9 cut(s)	147, 176, 415, 496, 547, 964, 1134, 1336, 1577
CviQI	GTAC	8 cut(s)	352, 393, 433, 439, 463, 737, 1333, 1345
DdeI	CTNAG	1 cut(s)	542
DinI	GGCGCC	1 cut(s)	759
DpnI	GATC	5 cut(s)	110, 551, 643, 1042, 1053
DpnII	GATC	5 cut(s)	108, 549, 641, 1040, 1051
DriI	GACNNNNNGTC	1 cut(s)	448
EaeI	YGGCCR	3 cut(s)	388, 966, 1536
Eam1104I	CTCTTC	3 cut(s)	807, 833, 1323
Eam1105I	GACNNNNNGTC	1 cut(s)	448
EarI	CTCTTC	3 cut(s)	807, 833, 1323
EciI	GGCGGA	5 cut(s)	49, 412, 442, 922, 1544
Ecl136II	GAGCTC	1 cut(s)	420
Eco130I	CCWWGG	2 cut(s)	1081, 1463
Eco24I	GRGCYC	2 cut(s)	422, 1001
Eco47I	GGWCC	4 cut(s)	450, 591, 732, 1520
Eco53kI	GAGCTC	1 cut(s)	420
Eco72I	CACGTG	1 cut(s)	386
Eco88I	CYCGRG	1 cut(s)	1516
EcoICRI	GAGCTC	1 cut(s)	420
EcoO109I	RGGNCCY	1 cut(s)	998
EcoRII	CCWGG	2 cut(s)	587, 1023
EcoT14I	CCWWGG	2 cut(s)	1081, 1463
EcoT38I	GRGCYC	2 cut(s)	422, 1001
EgeI	GGCGCC	1 cut(s)	759
EheI	GGCGCC	1 cut(s)	759
ErhI	CCWWGG	2 cut(s)	1081, 1463
Esp3I	CGTCTC	1 cut(s)	493
FaeI	CATG	9 cut(s)	150, 179, 418, 499, 550, 967, 1137, 1339, 1580
FaqI	GGGAC	2 cut(s)	637, 1533
FatI	CATG	9 cut(s)	146, 175, 414, 495, 546, 963, 1133, 1335, 1576
FauI	CCCGC	4 cut(s)	17, 140, 603, 988
FbaI	TGATCA	1 cut(s)	1051
FblI	GTMKAC	2 cut(s)	5, 1572
Fnu4HI	GCNGC	8 cut(s)	63, 288, 295, 315, 411, 756, 1179, 1195
FokI	GGATG	3 cut(s)	65, 880, 1187
FriOI	GRGCYC	2 cut(s)	422, 1001
Fsp4HI	GCNGC	8 cut(s)	63, 288, 295, 315, 411, 756, 1179, 1195
FspBI	CTAG	4 cut(s)	218, 833, 1082, 1636
GlaI	GCGC	4 cut(s)	611, 759, 1212, 1281
GluI	GCNGC	8 cut(s)	63, 288, 295, 315, 411, 756, 1179, 1195
GsaI	CCCAGC	1 cut(s)	1012
GsuI	CTGGAG	2 cut(s)	514, 642
HaeII	RGCGCY	1 cut(s)	761
HaeIII	GGCC	9 cut(s)	155, 243, 378, 390, 906, 968, 999, 1387, 1538
HgaI	GACGC	3 cut(s)	964, 1013, 1498
HhaI	GCGC	4 cut(s)	612, 760, 1213, 1282
Hin1I	GRCGYC	3 cut(s)	758, 975, 1005
Hin1II	CATG	9 cut(s)	150, 179, 418, 499, 550, 967, 1137, 1339, 1580
Hin6I	GCGC	4 cut(s)	610, 758, 1211, 1280
HinP1I	GCGC	4 cut(s)	610, 758, 1211, 1280
HinfI	GANTC	3 cut(s)	527, 787, 1564
HphI	GGTGA	4 cut(s)	358, 760, 1013, 1558
Hpy166II	GTNNAC	8 cut(s)	6, 526, 751, 949, 1021, 1225, 1366, 1573
Hpy188III	TCNNGA	5 cut(s)	659, 784, 1303, 1417, 1518
Hpy8I	GTNNAC	8 cut(s)	6, 526, 751, 949, 1021, 1225, 1366, 1573
Hpy99I	CGWCG	7 cut(s)	101, 242, 617, 959, 1007, 1193, 1492
HpyAV	CCTTC	4 cut(s)	191, 466, 1387, 1445
HpyCH4III	ACNGT	9 cut(s)	356, 523, 700, 736, 880, 940, 1049, 1411, 1424
HpyCH4IV	ACGT	3 cut(s)	99, 385, 693
HpyCH4V	TGCA	8 cut(s)	39, 123, 705, 1137, 1197, 1366, 1586, 1597
HpyF10VI	GCNNNNNNNGC	5 cut(s)	303, 410, 752, 1256, 1535
HpyF3I	CTNAG	1 cut(s)	542
HpySE526I	ACGT	3 cut(s)	99, 385, 693
Hsp92I	GRCGYC	3 cut(s)	758, 975, 1005
Hsp92II	CATG	9 cut(s)	150, 179, 418, 499, 550, 967, 1137, 1339, 1580
HspAI	GCGC	4 cut(s)	610, 758, 1211, 1280
KasI	GGCGCC	1 cut(s)	757
Kpn2I	TCCGGA	1 cut(s)	1302
KroI	GCCGGC	1 cut(s)	290
KroNI	GCCGGC	1 cut(s)	292
Ksp22I	TGATCA	1 cut(s)	1051
Kzo9I	GATC	5 cut(s)	108, 549, 641, 1040, 1051
LmnI	GCTCC	2 cut(s)	80, 425
Lsp1109I	GCAGC	3 cut(s)	306, 1165, 1181
LweI	GCATC	1 cut(s)	48
MaeI	CTAG	4 cut(s)	218, 833, 1082, 1636
MaeII	ACGT	3 cut(s)	99, 385, 693
MaeIII	GTNAC	5 cut(s)	631, 694, 725, 976, 1252
MalI	GATC	5 cut(s)	110, 551, 643, 1042, 1053
MbiI	CCGCTC	1 cut(s)	75
MboI	GATC	5 cut(s)	108, 549, 641, 1040, 1051
MboII	GAAGA	5 cut(s)	544, 824, 850, 1255, 1340
MhlI	GDGCHC	4 cut(s)	422, 1001, 1089, 1368
MlsI	TGGCCA	1 cut(s)	968
MluCI	AATT	7 cut(s)	343, 779, 882, 915, 1069, 1140, 1600
MluNI	TGGCCA	1 cut(s)	968
Mly113I	GGCGCC	1 cut(s)	758
MlyI	GAGTC	1 cut(s)	521
MmeI	TCCRAC	4 cut(s)	136, 261, 294, 795
Mox20I	TGGCCA	1 cut(s)	968
MroI	TCCGGA	1 cut(s)	1302
MroNI	GCCGGC	1 cut(s)	290
MroXI	GAANNNNTTC	1 cut(s)	1310
MscI	TGGCCA	1 cut(s)	968
MseI	TTAA	4 cut(s)	507, 720, 822, 1353
MslI	CAYNNNNRTG	1 cut(s)	1581
Msp20I	TGGCCA	1 cut(s)	968
MspR9I	CCNGG	7 cut(s)	589, 639, 802, 912, 1025, 1277, 1524
Mva1269I	GAATGC	1 cut(s)	1252
MvaI	CCWGG	2 cut(s)	589, 1025
MvnI	CGCG	3 cut(s)	12, 612, 755
MwoI	GCNNNNNNNGC	5 cut(s)	303, 410, 752, 1256, 1535
NaeI	GCCGGC	1 cut(s)	292
NarI	GGCGCC	1 cut(s)	758
NciI	CCSGG	5 cut(s)	639, 802, 912, 1277, 1524
NdeII	GATC	5 cut(s)	108, 549, 641, 1040, 1051
NgoMIV	GCCGGC	1 cut(s)	290
NlaIII	CATG	9 cut(s)	150, 179, 418, 499, 550, 967, 1137, 1339, 1580
NlaIV	GGNNCC	8 cut(s)	593, 627, 759, 828, 999, 1000, 1088, 1521
NmeAIII	GCCGAG	3 cut(s)	1154, 1239, 1564
NmuCI	GTSAC	4 cut(s)	631, 694, 976, 1252
NspI	RCATGY	1 cut(s)	150
NspV	TTCGAA	1 cut(s)	1445
PciI	ACATGT	1 cut(s)	146
PctI	GAATGC	1 cut(s)	1252
PdiI	GCCGGC	1 cut(s)	292
PdmI	GAANNNNTTC	1 cut(s)	1310
PfeI	GAWTC	2 cut(s)	787, 1564
PflMI	CCANNNNNTGG	2 cut(s)	588, 1030
PfoI	TCCNGGA	1 cut(s)	587
PkrI	GCNGC	8 cut(s)	64, 289, 296, 316, 412, 757, 1180, 1196
PleI	GAGTC	1 cut(s)	521
PluTI	GGCGCC	1 cut(s)	761
PmaCI	CACGTG	1 cut(s)	386
PmlI	CACGTG	1 cut(s)	386
PpsI	GAGTC	1 cut(s)	521
Ppu21I	YACGTR	1 cut(s)	386
PscI	ACATGT	1 cut(s)	146
PshAI	GACNNNNGTC	1 cut(s)	147
Psp124BI	GAGCTC	1 cut(s)	422
Psp6I	CCWGG	2 cut(s)	587, 1023
PspCI	CACGTG	1 cut(s)	386
PspFI	CCCAGC	1 cut(s)	1008
PspGI	CCWGG	2 cut(s)	587, 1023
PspN4I	GGNNCC	8 cut(s)	593, 627, 759, 828, 999, 1000, 1088, 1521
PspOMI	GGGCCC	1 cut(s)	997
PspPI	GGNCC	8 cut(s)	153, 450, 591, 732, 904, 997, 998, 1520
PsrI	GAACNNNNNNTAC	2 cut(s)	654, 686
RsaI	GTAC	8 cut(s)	353, 394, 434, 440, 464, 738, 1334, 1346
RsaNI	GTAC	8 cut(s)	352, 393, 433, 439, 463, 737, 1333, 1345
RseI	CAYNNNNRTG	1 cut(s)	1581
SacI	GAGCTC	1 cut(s)	422
SaqAI	TTAA	4 cut(s)	507, 720, 822, 1353
SatI	GCNGC	8 cut(s)	63, 288, 295, 315, 411, 756, 1179, 1195
Sau3AI	GATC	5 cut(s)	108, 549, 641, 1040, 1051
Sau96I	GGNCC	8 cut(s)	153, 450, 591, 732, 904, 997, 998, 1520
SchI	GAGTC	1 cut(s)	521
ScrFI	CCNGG	7 cut(s)	589, 639, 802, 912, 1025, 1277, 1524
SduI	GDGCHC	4 cut(s)	422, 1001, 1089, 1368
SfaNI	GCATC	1 cut(s)	48
SfcI	CTRYAG	1 cut(s)	1423
SfoI	GGCGCC	1 cut(s)	759
SfuI	TTCGAA	1 cut(s)	1445
SinI	GGWCC	4 cut(s)	450, 591, 732, 1520
SmiMI	CAYNNNNRTG	1 cut(s)	1581
Sse9I	AATT	7 cut(s)	343, 779, 882, 915, 1069, 1140, 1600
SspDI	GGCGCC	1 cut(s)	757
SspMI	CTAG	4 cut(s)	218, 833, 1082, 1636
SstI	GAGCTC	1 cut(s)	422
StyD4I	CCNGG	7 cut(s)	587, 637, 800, 910, 1023, 1275, 1522
StyI	CCWWGG	2 cut(s)	1081, 1463
TaaI	ACNGT	9 cut(s)	356, 523, 700, 736, 880, 940, 1049, 1411, 1424
TaiI	ACGT	3 cut(s)	102, 388, 696
TaqI	TCGA	6 cut(s)	330, 668, 920, 1188, 1403, 1445
TaqII	GACCGA	1 cut(s)	620
TasI	AATT	7 cut(s)	343, 779, 882, 915, 1069, 1140, 1600
TatI	WGTACW	2 cut(s)	1332, 1344
TauI	GCSGC	5 cut(s)	65, 290, 317, 413, 758
TfiI	GAWTC	2 cut(s)	787, 1564
Tru1I	TTAA	4 cut(s)	507, 720, 822, 1353
Tru9I	TTAA	4 cut(s)	507, 720, 822, 1353
TscAI	CASTG	1 cut(s)	1054
TseFI	GTSAC	4 cut(s)	631, 694, 976, 1252
TseI	GCWGC	3 cut(s)	294, 1178, 1194
Tsp45I	GTSAC	4 cut(s)	631, 694, 976, 1252
TspDTI	ATGAA	5 cut(s)	493, 825, 952, 1303, 1352
TspGWI	ACGGA	2 cut(s)	1335, 1340
TspRI	CASTG	1 cut(s)	1054
Van91I	CCANNNNNTGG	2 cut(s)	588, 1030
VneI	GTGCAC	1 cut(s)	1364
VpaK11BI	GGWCC	4 cut(s)	450, 591, 732, 1520
XapI	RAATTY	5 cut(s)	343, 779, 915, 1140, 1600
XceI	RCATGY	1 cut(s)	150
XmaJI	CCTAGG	1 cut(s)	1081
XmiI	GTMKAC	2 cut(s)	5, 1572
XmnI	GAANNNNTTC	1 cut(s)	1310
XspI	CTAG	4 cut(s)	218, 833, 1082, 1636

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.