Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Reverse (-)

31639462 .. 31641978

Gene Structure 2517 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018546

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGACCACCCACCACAGAAAACCCATAAAACCCAGCTTCGCCTCACTCTCCAAACCATGTTCCTCCCTATCCAAACCCTCCAAATTCCTCTTCATCTCCATGGCCTCCTTCGTCTTCCTCTCCCTCCTAACCCTCACTCTCTTCCTCTCCCTCTCCTCCGCCGCCCGCCACCGTCACCCTTCCACCAACGCCGCCGCCGGCGCTCAGATCCAACTCGCCTGCAAAGCCACCCGCTTCCCCGACACGTGTCAATCCTCCCTCGCCGAGCTCCCTGACCTTCCTCCCAACCCGACCCCAATCGAGACCATCGGAATAGCCATCCGGGTCTCCTCCGATGGGCTTAAGTCGGCCCAGAAAATGGTGACGGACATTCGAGAAGCGTCCAAGGGGAACCAGAACCGCACCCTCGCCGCGAACAACTGCCTCGAGGTTCTGCACAACTCCGAGTACCGCATTTCGCGGGCGACCGAGGAGCTCTCACGTGGGCGGACCAAGAGCGCACGCGCCGCCTTCAGCGCCGCCCTGCTCTACCAGTACGACTGCTGGTCGGGCCTCAAGTACGCCAACGACACCCAGATGGTCAACAAAACGATGTCGTTTCTCGACGGGCTGCAAAGCAGGTCCAGCAACGCCCTCAGCATGATGTTCTCCTACGACAACTACGGCAACGACACCAGCCTGTGGGCCCCGCCGAAGACCGAGCGTGACGGGTTCTGGGAGCGGGGGTCGGGTTCGGGTTTCTCGGGCGGCGTGCCGAAGAATCTGAAGGCGGACGCGACGGTGTGTAAGGAGGGGTGTGAGTACCGGACGGTGCAGGAGGCGGTGGACAAGGCGCCGGAGAATTTGGAGGCAGGGAAGAGGTTCGTGATTCAGATAAAGACGGGGGTGTACGAGGAGACGGTGAGGGTGGCCTTAGAGAAGCGGAACCTGGTGTTCTTGGGCGACGGGATGGGCAAGACGGTAATTACGGGTTCGTTGAATGTGGGCCGGCCTGGAATCTCCACCTACAATACGGCCACCGTCGGAGTTCTTGGTGATGGGTTCATGGCCAGTGGTCTCACAATTGCAAACACAGCAGGCCCTGATGCCCACCAGGCAGTGGCTTTCCGATCAGACAGCGATCTATCCGTGGTCGAGAACTGTGAATTCTTAGGAAATCAGGACACTCTATATGCACATGGCAACCGTCAGCTATACAAATCGTGCAAAATCCAAGGCAATGTGGATTTCATCTTCGGAAACTCAGCTGCCATTTTCCAGGACTGCAACATCCTTGTTGCTCCCCGACAACTCAATCCCGAGAAGGGCGAAACCAATGCCATCACAGCCCATGGCAGGACAGACCCTGCACAGTCAACCGGTTTCGTTTTTCAAAATTGCTTGATCAACGGAACCGAAGAGTACATGAAATTGTATTACAGCAAACCCAAAGTGCACAAAAACTACTTGGGGAGGCCGTGGAAGGAGTATTCAAGGACGGTTTTCTTAAATTGCAATATGGAAGCTCTGGTTACCGCGCAGGGGTGGCTGCCTTGGAGTGGGGATTTTGCTTTGAAGACACTCTTCTATGGGGAGTACGGGAATACAGGTACAGGTTCGAATTTGAACCAGAGAGTGACCTGGAGCAGCAAGATTCCGACCGAGCATGTTAGTACATATTCAGTGCAGAATTTTATTCAGGGAGATGAGTGGATGGAGACCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

569

Amino Acids

62.35

Weight (kDa)

8.57

Isoelectric Point (pI)

28.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	69 - 214	1.2e-22	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	258 - 562	4.3e-117	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	425
Acc36I	ACCTGC	1 cut(s)	609
AccB1I	GGYRCC	1 cut(s)	832
AccB7I	CCANNNNNTGG	3 cut(s)	358, 681, 1099
AccBSI	CCGCTC	1 cut(s)	721
AccII	CGCG	5 cut(s)	413, 460, 504, 776, 1517
AclWI	GGATC	1 cut(s)	202
AcoI	YGGCCR	2 cut(s)	1014, 1047
AcsI	RAATTY	5 cut(s)	83, 841, 1145, 1600, 1669
AcuI	CTGAAG	2 cut(s)	496, 785
AcvI	CACGTG	2 cut(s)	246, 482
AcyI	GRCGYC	1 cut(s)	833
AfaI	GTAC	9 cut(s)	449, 536, 560, 803, 890, 1403, 1577, 1591, 1654
AfiI	CCNNNNNNNGG	8 cut(s)	336, 358, 681, 1099, 1304, 1335, 1521, 1538
AflII	CTTAAG	1 cut(s)	341
AflIII	ACRYGT	2 cut(s)	243, 245
AgeI	ACCGGT	1 cut(s)	1358
AgsI	TTSAA	5 cut(s)	979, 1373, 1473, 1555, 1606
AhdI	GACNNNNNGTC	1 cut(s)	544
AjnI	CCWGG	5 cut(s)	927, 991, 1092, 1257, 1619
AjuI	GAANNNNNNNTTGG	2 cut(s)	74, 106
AluBI	AGCT	6 cut(s)	36, 268, 475, 1192, 1247, 1505
AluI	AGCT	6 cut(s)	36, 268, 475, 1192, 1247, 1505
Alw21I	GWGCWC	3 cut(s)	270, 477, 1437
Alw26I	GTCTC	5 cut(s)	296, 331, 890, 1061, 1691
Alw44I	GTGCAC	1 cut(s)	1433
AlwI	GGATC	1 cut(s)	202
AlwNI	CAGNNNCTG	2 cut(s)	1082, 1346
Ama87I	CYCGRG	3 cut(s)	425, 742, 1298
ApaI	GGGCCC	1 cut(s)	688
ApaLI	GTGCAC	1 cut(s)	1433
ApeKI	GCWGC	4 cut(s)	610, 1247, 1528, 1626
ApoI	RAATTY	5 cut(s)	83, 841, 1145, 1600, 1669
AsiGI	ACCGGT	1 cut(s)	1358
Asp700I	GAANNNNTTC	1 cut(s)	860
AspLEI	GCGC	6 cut(s)	203, 500, 506, 518, 835, 1519
AspS9I	GGNCC	8 cut(s)	349, 489, 550, 621, 684, 685, 985, 1079
AsuC2I	CCSGG	1 cut(s)	323
AsuHPI	GGTGA	4 cut(s)	167, 373, 913, 1046
AsuII	TTCGAA	1 cut(s)	1598
AvaI	CYCGRG	3 cut(s)	425, 742, 1298
AvaII	GGWCC	2 cut(s)	489, 621
BaeGI	GKGCMC	2 cut(s)	688, 1437
BalI	TGGCCA	1 cut(s)	1049
BanI	GGYRCC	1 cut(s)	832
BanII	GRGCYC	3 cut(s)	270, 477, 688
BbrPI	CACGTG	2 cut(s)	246, 482
BbsI	GAAGAC	3 cut(s)	106, 701, 1562
Bbv12I	GWGCWC	3 cut(s)	270, 477, 1437
BbvCI	CCTCAGC	1 cut(s)	635
BbvI	GCAGC	4 cut(s)	597, 1234, 1515, 1638
BccI	CCATC	8 cut(s)	314, 326, 329, 571, 943, 1031, 1328, 1687
BceAI	ACGGC	3 cut(s)	679, 1029, 1441
BcgI	CGANNNNNNTGC	2 cut(s)	1298, 1332
BciT130I	CCWGG	5 cut(s)	929, 993, 1094, 1259, 1621
BclI	TGATCA	1 cut(s)	1383
BcnI	CCSGG	1 cut(s)	323
BcoDI	GTCTC	5 cut(s)	296, 331, 890, 1061, 1691
BfoI	RGCGCY	3 cut(s)	204, 519, 836
BfrI	CTTAAG	1 cut(s)	341
BfuAI	ACCTGC	1 cut(s)	609
BglI	GCCNNNNNGGC	1 cut(s)	1094
Bme1390I	CCNGG	6 cut(s)	323, 929, 993, 1094, 1259, 1621
Bme18I	GGWCC	2 cut(s)	489, 621
BmeRI	GACNNNNNGTC	1 cut(s)	544
BmeT110I	CYCGRG	3 cut(s)	425, 742, 1298
BmgT120I	GGNCC	8 cut(s)	349, 489, 550, 621, 684, 685, 985, 1079
BmiI	GGNNCC	6 cut(s)	392, 686, 687, 834, 926, 1393
BmrFI	CCNGG	6 cut(s)	323, 929, 993, 1094, 1259, 1621
BmsI	GCATC	1 cut(s)	1075
BoxI	GACNNNNGTC	1 cut(s)	246
BpiI	GAAGAC	3 cut(s)	106, 701, 1562
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
BpmI	CTGGAG	1 cut(s)	1642
Bpu10I	CCTNAGC	1 cut(s)	635
Bpu14I	TTCGAA	1 cut(s)	1598
BpuEI	CTTGAG	1 cut(s)	539
BpuMI	CCSGG	1 cut(s)	323
BsaAI	YACGTR	2 cut(s)	246, 482
BsaHI	GRCGYC	1 cut(s)	833
BsaI	GGTCTC	4 cut(s)	296, 331, 1061, 1691
BsaJI	CCNNGG	8 cut(s)	99, 384, 468, 1128, 1213, 1330, 1457, 1532
BsaWI	WCCGGW	2 cut(s)	804, 1358
BsaXI	ACNNNNNCTCC	2 cut(s)	710, 740
Bsc4I	CCNNNNNNNGG	8 cut(s)	336, 358, 681, 1099, 1304, 1335, 1521, 1538
Bse118I	RCCGGY	3 cut(s)	197, 987, 1358
Bse1I	ACTGG	2 cut(s)	532, 1050
Bse3DI	GCAATG	1 cut(s)	1225
BseBI	CCWGG	5 cut(s)	929, 993, 1094, 1259, 1621
BseDI	CCNNGG	8 cut(s)	99, 384, 468, 1128, 1213, 1330, 1457, 1532
BseGI	GGATG	4 cut(s)	318, 954, 1269, 1698
BseLI	CCNNNNNNNGG	8 cut(s)	336, 358, 681, 1099, 1304, 1335, 1521, 1538
BseMI	GCAATG	1 cut(s)	1225
BseMII	CTCAG	3 cut(s)	218, 649, 1257
BseNI	ACTGG	2 cut(s)	532, 1050
BseRI	GAGGAG	4 cut(s)	145, 319, 485, 908
BseSI	GKGCMC	2 cut(s)	688, 1437
BseXI	GCAGC	4 cut(s)	597, 1234, 1515, 1638
BseYI	CCCAGC	1 cut(s)	32
BsgI	GTGCAG	4 cut(s)	419, 833, 1332, 1685
Bsh1236I	CGCG	5 cut(s)	413, 460, 504, 776, 1517
Bsh1285I	CGRYCG	2 cut(s)	468, 1641
BshNI	GGYRCC	1 cut(s)	832
BshTI	ACCGGT	1 cut(s)	1358
BsiEI	CGRYCG	2 cut(s)	468, 1641
BsiHKAI	GWGCWC	3 cut(s)	270, 477, 1437
BsiHKCI	CYCGRG	3 cut(s)	425, 742, 1298
BsiSI	CCGG	6 cut(s)	198, 322, 805, 836, 988, 1359
BslI	CCNNNNNNNGG	8 cut(s)	336, 358, 681, 1099, 1304, 1335, 1521, 1538
BsmAI	GTCTC	5 cut(s)	296, 331, 890, 1061, 1691
BsmBI	CGTCTC	1 cut(s)	890
Bso31I	GGTCTC	4 cut(s)	296, 331, 1061, 1691
BsoBI	CYCGRG	3 cut(s)	425, 742, 1298
Bsp119I	TTCGAA	1 cut(s)	1598
Bsp120I	GGGCCC	1 cut(s)	684
Bsp1286I	GDGCHC	4 cut(s)	270, 477, 688, 1437
Bsp143I	GATC	4 cut(s)	207, 1109, 1120, 1383
Bsp19I	CCATGG	2 cut(s)	99, 1330
BspCNI	CTCAG	3 cut(s)	217, 648, 1256
BspFNI	CGCG	5 cut(s)	413, 460, 504, 776, 1517
BspLI	GGNNCC	6 cut(s)	392, 686, 687, 834, 926, 1393
BspMI	ACCTGC	1 cut(s)	609
BspPI	GGATC	1 cut(s)	202
BspT104I	TTCGAA	1 cut(s)	1598
BspT107I	GGYRCC	1 cut(s)	832
BspTI	CTTAAG	1 cut(s)	341
BspTNI	GGTCTC	4 cut(s)	296, 331, 1061, 1691
BsrBI	CCGCTC	1 cut(s)	721
BsrDI	GCAATG	1 cut(s)	1225
BsrFI	RCCGGY	3 cut(s)	197, 987, 1358
BsrI	ACTGG	2 cut(s)	532, 1050
BssAI	RCCGGY	3 cut(s)	197, 987, 1358
BssECI	CCNNGG	8 cut(s)	99, 384, 468, 1128, 1213, 1330, 1457, 1532
BssMI	GATC	4 cut(s)	207, 1109, 1120, 1383
BssNI	GRCGYC	1 cut(s)	833
BssT1I	CCWWGG	5 cut(s)	99, 384, 1213, 1330, 1532
Bst2UI	CCWGG	5 cut(s)	929, 993, 1094, 1259, 1621
Bst6I	CTCTTC	5 cut(s)	95, 146, 851, 1392, 1568
BstACI	GRCGYC	1 cut(s)	833
BstAFI	CTTAAG	1 cut(s)	341
BstBAI	YACGTR	2 cut(s)	246, 482
BstBI	TTCGAA	1 cut(s)	1598
BstC8I	GCNNGC	8 cut(s)	166, 199, 220, 462, 502, 752, 989, 1078
BstDEI	CTNAG	5 cut(s)	204, 635, 913, 1150, 1243
BstDSI	CCRYGG	4 cut(s)	99, 1128, 1330, 1457
BstEII	GGTNACC	1 cut(s)	1510
BstF5I	GGATG	4 cut(s)	318, 954, 1269, 1698
BstFNI	CGCG	5 cut(s)	413, 460, 504, 776, 1517
BstH2I	RGCGCY	3 cut(s)	204, 519, 836
BstHHI	GCGC	6 cut(s)	203, 500, 506, 518, 835, 1519
BstKTI	GATC	4 cut(s)	210, 1112, 1123, 1386
BstMAI	GTCTC	5 cut(s)	296, 331, 890, 1061, 1691
BstMBI	GATC	4 cut(s)	207, 1109, 1120, 1383
BstMCI	CGRYCG	2 cut(s)	468, 1641
BstNI	CCWGG	5 cut(s)	929, 993, 1094, 1259, 1621
BstNSI	RCATGY	1 cut(s)	1649
BstPAI	GACNNNNGTC	1 cut(s)	246
BstPI	GGTNACC	1 cut(s)	1510
BstSCI	CCNGG	6 cut(s)	321, 927, 991, 1092, 1257, 1619
BstSLI	GKGCMC	2 cut(s)	688, 1437
BstUI	CGCG	5 cut(s)	413, 460, 504, 776, 1517
BstV1I	GCAGC	4 cut(s)	597, 1234, 1515, 1638
BstV2I	GAAGAC	3 cut(s)	106, 701, 1562
BstX2I	RGATCY	1 cut(s)	207
BstYI	RGATCY	1 cut(s)	207
BtgI	CCRYGG	4 cut(s)	99, 1128, 1330, 1457
BtsCI	GGATG	4 cut(s)	318, 954, 1269, 1698
BtsI	GCAGTG	1 cut(s)	1104
BtsIMutI	CAGTG	3 cut(s)	1057, 1104, 1668
BveI	ACCTGC	1 cut(s)	609
Cac8I	GCNNGC	8 cut(s)	166, 199, 220, 462, 502, 752, 989, 1078
CaiI	CAGNNNCTG	2 cut(s)	1082, 1346
CfoI	GCGC	6 cut(s)	203, 500, 506, 518, 835, 1519
Cfr10I	RCCGGY	3 cut(s)	197, 987, 1358
Cfr13I	GGNCC	8 cut(s)	349, 489, 550, 621, 684, 685, 985, 1079
CseI	GACGC	2 cut(s)	369, 782
CsiI	ACCWGGT	1 cut(s)	927
Csp6I	GTAC	9 cut(s)	448, 535, 559, 802, 889, 1402, 1576, 1590, 1653
CspAI	ACCGGT	1 cut(s)	1358
CviAII	CATG	8 cut(s)	57, 100, 640, 1045, 1178, 1331, 1405, 1646
CviQI	GTAC	9 cut(s)	448, 535, 559, 802, 889, 1402, 1576, 1590, 1653
DdeI	CTNAG	5 cut(s)	204, 635, 913, 1150, 1243
DinI	GGCGCC	1 cut(s)	834
DpnI	GATC	4 cut(s)	209, 1111, 1122, 1385
DpnII	GATC	4 cut(s)	207, 1109, 1120, 1383
DriI	GACNNNNNGTC	1 cut(s)	544
EaeI	YGGCCR	2 cut(s)	1014, 1047
Eam1104I	CTCTTC	5 cut(s)	95, 146, 851, 1392, 1568
Eam1105I	GACNNNNNGTC	1 cut(s)	544
EarI	CTCTTC	5 cut(s)	95, 146, 851, 1392, 1568
EciI	GGCGGA	3 cut(s)	148, 502, 785
Ecl136II	GAGCTC	2 cut(s)	268, 475
Eco130I	CCWWGG	5 cut(s)	99, 384, 1213, 1330, 1532
Eco24I	GRGCYC	3 cut(s)	270, 477, 688
Eco31I	GGTCTC	4 cut(s)	296, 331, 1061, 1691
Eco47I	GGWCC	2 cut(s)	489, 621
Eco53kI	GAGCTC	2 cut(s)	268, 475
Eco57I	CTGAAG	2 cut(s)	496, 785
Eco72I	CACGTG	2 cut(s)	246, 482
Eco88I	CYCGRG	3 cut(s)	425, 742, 1298
Eco91I	GGTNACC	1 cut(s)	1510
EcoICRI	GAGCTC	2 cut(s)	268, 475
EcoO109I	RGGNCCY	2 cut(s)	685, 1079
EcoO65I	GGTNACC	1 cut(s)	1510
EcoRI	GAATTC	1 cut(s)	1145
EcoRII	CCWGG	5 cut(s)	927, 991, 1092, 1257, 1619
EcoT14I	CCWWGG	5 cut(s)	99, 384, 1213, 1330, 1532
EcoT38I	GRGCYC	3 cut(s)	270, 477, 688
EgeI	GGCGCC	1 cut(s)	834
EheI	GGCGCC	1 cut(s)	834
ErhI	CCWWGG	5 cut(s)	99, 384, 1213, 1330, 1532
Esp3I	CGTCTC	1 cut(s)	890
FaeI	CATG	8 cut(s)	60, 103, 643, 1048, 1181, 1334, 1408, 1649
FalI	AAGNNNNNCTT	2 cut(s)	1547, 1579
FatI	CATG	8 cut(s)	56, 99, 639, 1044, 1177, 1330, 1404, 1645
FauI	CCCGC	5 cut(s)	173, 239, 453, 696, 714
FbaI	TGATCA	1 cut(s)	1383
FokI	GGATG	3 cut(s)	305, 961, 1256
FriOI	GRGCYC	3 cut(s)	270, 477, 688
FseI	GGCCGGCC	1 cut(s)	991
GlaI	GCGC	6 cut(s)	202, 499, 505, 517, 834, 1518
GsaI	CCCAGC	1 cut(s)	36
GsuI	CTGGAG	1 cut(s)	1642
HaeII	RGCGCY	3 cut(s)	204, 519, 836
HapII	CCGG	6 cut(s)	198, 322, 805, 836, 988, 1359
HgaI	GACGC	2 cut(s)	369, 782
HhaI	GCGC	6 cut(s)	203, 500, 506, 518, 835, 1519
Hin1I	GRCGYC	1 cut(s)	833
Hin1II	CATG	8 cut(s)	60, 103, 643, 1048, 1181, 1334, 1408, 1649
Hin6I	GCGC	6 cut(s)	201, 498, 504, 516, 833, 1517
HinP1I	GCGC	6 cut(s)	201, 498, 504, 516, 833, 1517
HincII	GTYRAC	2 cut(s)	583, 1356
HindII	GTYRAC	2 cut(s)	583, 1356
HinfI	GANTC	4 cut(s)	760, 868, 996, 1633
HpaII	CCGG	6 cut(s)	198, 322, 805, 836, 988, 1359
HphI	GGTGA	4 cut(s)	167, 373, 913, 1046
Hpy166II	GTNNAC	5 cut(s)	583, 826, 889, 1356, 1435
Hpy188III	TCNNGA	7 cut(s)	301, 374, 602, 865, 1135, 1160, 1298
Hpy8I	GTNNAC	5 cut(s)	583, 826, 889, 1356, 1435
Hpy99I	CGWCG	4 cut(s)	608, 781, 947, 1025
HpyAV	CCTTC	7 cut(s)	118, 189, 287, 520, 760, 1297, 1456
HpyCH4IV	ACGT	2 cut(s)	245, 481
HpyF3I	CTNAG	5 cut(s)	204, 635, 913, 1150, 1243
HpySE526I	ACGT	2 cut(s)	245, 481
Hsp92I	GRCGYC	1 cut(s)	833
Hsp92II	CATG	8 cut(s)	60, 103, 643, 1048, 1181, 1334, 1408, 1649
HspAI	GCGC	6 cut(s)	201, 498, 504, 516, 833, 1517
KasI	GGCGCC	1 cut(s)	832
KroI	GCCGGC	2 cut(s)	197, 987
KroNI	GCCGGC	2 cut(s)	199, 989
Ksp22I	TGATCA	1 cut(s)	1383
Kzo9I	GATC	4 cut(s)	207, 1109, 1120, 1383
LmnI	GCTCC	5 cut(s)	273, 472, 718, 1285, 1623
Lsp1109I	GCAGC	4 cut(s)	597, 1234, 1515, 1638
LweI	GCATC	1 cut(s)	1075
MabI	ACCWGGT	1 cut(s)	927
MaeII	ACGT	2 cut(s)	245, 481
MaeIII	GTNAC	5 cut(s)	173, 361, 704, 1510, 1615
MalI	GATC	4 cut(s)	209, 1111, 1122, 1385
MbiI	CCGCTC	1 cut(s)	721
MboI	GATC	4 cut(s)	207, 1109, 1120, 1383
MfeI	CAATTG	1 cut(s)	1062
MflI	RGATCY	1 cut(s)	207
MhlI	GDGCHC	4 cut(s)	270, 477, 688, 1437
MlsI	TGGCCA	1 cut(s)	1049
MluNI	TGGCCA	1 cut(s)	1049
Mly113I	GGCGCC	1 cut(s)	833
MmeI	TCCRAC	3 cut(s)	235, 1003, 1661
Mox20I	TGGCCA	1 cut(s)	1049
MreI	CGCCGGCG	1 cut(s)	197
MroNI	GCCGGC	2 cut(s)	197, 987
MroXI	GAANNNNTTC	1 cut(s)	860
MscI	TGGCCA	1 cut(s)	1049
MseI	TTAA	2 cut(s)	342, 1487
MslI	CAYNNNNRTG	2 cut(s)	98, 575
Msp20I	TGGCCA	1 cut(s)	1049
MspA1I	CMGCKG	1 cut(s)	1247
MspCI	CTTAAG	1 cut(s)	341
MspI	CCGG	6 cut(s)	198, 322, 805, 836, 988, 1359
MspR9I	CCNGG	6 cut(s)	323, 929, 993, 1094, 1259, 1621
MunI	CAATTG	1 cut(s)	1062
MvaI	CCWGG	5 cut(s)	929, 993, 1094, 1259, 1621
MvnI	CGCG	5 cut(s)	413, 460, 504, 776, 1517
NaeI	GCCGGC	2 cut(s)	199, 989
NarI	GGCGCC	1 cut(s)	833
NciI	CCSGG	1 cut(s)	323
NcoI	CCATGG	2 cut(s)	99, 1330
NdeII	GATC	4 cut(s)	207, 1109, 1120, 1383
NgoMIV	GCCGGC	2 cut(s)	197, 987
NlaIII	CATG	8 cut(s)	60, 103, 643, 1048, 1181, 1334, 1408, 1649
NlaIV	GGNNCC	6 cut(s)	392, 686, 687, 834, 926, 1393
NmeAIII	GCCGAG	1 cut(s)	289
NmuCI	GTSAC	4 cut(s)	173, 361, 704, 1615
NspI	RCATGY	1 cut(s)	1649
NspV	TTCGAA	1 cut(s)	1598
PaeR7I	CTCGAG	1 cut(s)	425
PdiI	GCCGGC	2 cut(s)	199, 989
PdmI	GAANNNNTTC	1 cut(s)	860
PfeI	GAWTC	4 cut(s)	760, 868, 996, 1633
PflMI	CCANNNNNTGG	3 cut(s)	358, 681, 1099
PfoI	TCCNGGA	1 cut(s)	1257
PinAI	ACCGGT	1 cut(s)	1358
PluTI	GGCGCC	1 cut(s)	836
PmaCI	CACGTG	2 cut(s)	246, 482
PmlI	CACGTG	2 cut(s)	246, 482
Ppu21I	YACGTR	2 cut(s)	246, 482
PshAI	GACNNNNGTC	1 cut(s)	246
Psp124BI	GAGCTC	2 cut(s)	270, 477
Psp6I	CCWGG	5 cut(s)	927, 991, 1092, 1257, 1619
PspCI	CACGTG	2 cut(s)	246, 482
PspEI	GGTNACC	1 cut(s)	1510
PspFI	CCCAGC	1 cut(s)	32
PspGI	CCWGG	5 cut(s)	927, 991, 1092, 1257, 1619
PspN4I	GGNNCC	6 cut(s)	392, 686, 687, 834, 926, 1393
PspOMI	GGGCCC	1 cut(s)	684
PspPI	GGNCC	8 cut(s)	349, 489, 550, 621, 684, 685, 985, 1079
PspXI	VCTCGAGB	1 cut(s)	425
PstNI	CAGNNNCTG	2 cut(s)	1082, 1346
PsuI	RGATCY	1 cut(s)	207
PvuII	CAGCTG	1 cut(s)	1247
RigI	GGCCGGCC	1 cut(s)	991
RsaI	GTAC	9 cut(s)	449, 536, 560, 803, 890, 1403, 1577, 1591, 1654
RsaNI	GTAC	9 cut(s)	448, 535, 559, 802, 889, 1402, 1576, 1590, 1653
RseI	CAYNNNNRTG	2 cut(s)	98, 575
SacI	GAGCTC	2 cut(s)	270, 477
SaqAI	TTAA	2 cut(s)	342, 1487
Sau3AI	GATC	4 cut(s)	207, 1109, 1120, 1383
Sau96I	GGNCC	8 cut(s)	349, 489, 550, 621, 684, 685, 985, 1079
ScrFI	CCNGG	6 cut(s)	323, 929, 993, 1094, 1259, 1621
SduI	GDGCHC	4 cut(s)	270, 477, 688, 1437
SexAI	ACCWGGT	1 cut(s)	927
SfaNI	GCATC	1 cut(s)	1075
SfoI	GGCGCC	1 cut(s)	834
Sfr274I	CTCGAG	1 cut(s)	425
SfuI	TTCGAA	1 cut(s)	1598
SgrAI	CRCCGGYG	1 cut(s)	197
SinI	GGWCC	2 cut(s)	489, 621
SlaI	CTCGAG	1 cut(s)	425
SmiMI	CAYNNNNRTG	2 cut(s)	98, 575
SmlI	CTYRAG	3 cut(s)	341, 425, 554
SmoI	CTYRAG	3 cut(s)	341, 425, 554
SspDI	GGCGCC	1 cut(s)	832
SstI	GAGCTC	2 cut(s)	270, 477
StyD4I	CCNGG	6 cut(s)	321, 927, 991, 1092, 1257, 1619
StyI	CCWWGG	5 cut(s)	99, 384, 1213, 1330, 1532
TaiI	ACGT	2 cut(s)	248, 484
TaqI	TCGA	6 cut(s)	300, 373, 426, 603, 1134, 1598
TaqII	GACCGA	3 cut(s)	482, 713, 1655
TatI	WGTACW	2 cut(s)	1401, 1652
TauI	GCSGC	7 cut(s)	164, 194, 197, 413, 509, 521, 750
TfiI	GAWTC	4 cut(s)	760, 868, 996, 1633
Tru1I	TTAA	2 cut(s)	342, 1487
Tru9I	TTAA	2 cut(s)	342, 1487
TscAI	CASTG	3 cut(s)	1057, 1104, 1668
TseFI	GTSAC	4 cut(s)	173, 361, 704, 1615
TseI	GCWGC	4 cut(s)	610, 1247, 1528, 1626
Tsp45I	GTSAC	4 cut(s)	173, 361, 704, 1615
TspDTI	ATGAA	4 cut(s)	82, 1033, 1219, 1421
TspGWI	ACGGA	3 cut(s)	380, 1117, 1404
TspRI	CASTG	3 cut(s)	1057, 1104, 1668
Van91I	CCANNNNNTGG	3 cut(s)	358, 681, 1099
Vha464I	CTTAAG	1 cut(s)	341
VneI	GTGCAC	1 cut(s)	1433
VpaK11BI	GGWCC	2 cut(s)	489, 621
XapI	RAATTY	5 cut(s)	83, 841, 1145, 1600, 1669
XceI	RCATGY	1 cut(s)	1649
XhoI	CTCGAG	1 cut(s)	425
XmnI	GAANNNNTTC	1 cut(s)	860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.