Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Forward (+)

7476181 .. 7478637

Gene Structure 2457 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1077900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGGCCTCCCTTCTCTTCCTCTCTCTCCTCTCCCTCACTCTCTTCCTCTCCCTCACTCTCTTCCTCTCCCTCTCCTCCGCCTCACGCCACCACTCCATCTCCCCCCAACCCCAGATCCAGCTAGCCTGCAAGGCCACACGCTTCCCCGACGCGTGCCAAGCCTCCCTGGGCAAGCTCGTCACTGACCCAAACGCCACCCCTCTCGAGACTATTCATTTCGCCGTCAAGGTCTCTGATGACGGCCTCAAGACCGCGCAGGGCATGGTCCACACTATCCTGGACTCGTCTGCGGGGAACATAAACCGCACCACCGCCGCGAAAAACTGCCTCGACGTCCTCGCCAACTCCCGGTACCGAATTTCTCTCACCACCGGTGGATTGTCAAGTGGTCGCGTTAAAAACGCACGTGCCTCCATGAGTGCCGCGTTGCTCTACCAATACGACTGCTGGTCGGCTCTCAAGTACGCAAACGACACCCAGATGGTCAACAAGACGATGTCGTTTCTCGACTCGCTGATCGGAAAATCCAGCAACGCGCTCGGCATGATTTGGTCGTACGACAACTTCGGAAATGACACTAAGAAGTGGGGGCCGCCGAAAACCGAGCGGGACGGGTTCTGGGAGCCGGTCATGGGCGGAGGGTCCGACCAGGGATTTCGGGGCGGGATTCCGTCGGGTTTAAAGGCGGACGTGACGGTGTGCAAGGGGAATTCGTGTGATTACAAGACGGTGCAGGAGGCGGTGAATGCCGCGCCGGATAACGCGGGAGATAAGAGGTTCGTGATTGGGATAAAGGCTGGGGTTTACGAGGAGACCGTGGGGGTGCCCCTAGAGAAGCGGAACGTGGTATTCTTGGGTGACGGGATGGGCAAAACGGTCATTACCGGGTCGTTGAATGTGGGCCAGCCCGGAATATCCACCTACAACACTGCCACCGTCGGAGTTAGTGGTGATGGGTTCATGGCGAGTGGTCTCACAGTCCAGAACACAGCAGGTCCCGACGCCCACCAAGCAGTTGCTTTCAGATCAGACAGCGACCTCTCCGTAATCGAAAACTGCGAATTCATAGGCAATCAGGACACTCTCTATGCTCATGCCAACCGCCAATTCTACAAGTCGTGCACCATCCAAGGCAATGTGGATTTCATCTTCGGAAACTCTGCCTCCATCTTCCAGGACTGCACCATCCTTGTTCGTCCTAGACAGCTCGAACCCGAGAAGGGCGAAAACAATGCAGTCACAGCCCATGGAAGAACAGACCCCGGGCAGTCAACGGGCTTTGTATTTGAAAACTGCTTGATCAATGGCACGGAAGAATACATGAAGTTGTATCGGAGCAAGCCGCAGGTGCACAAGAACTACTTGGGGAGGCCGTGGAAGGAGTACTCGAGGACGGTTTTCATAAACTCGAGTATGGAAGCTCTTGTTGCACCGCAAGGGTGGATGCCTTGGGATGGGGATTTCGCTTTGAACACCCTTTTCTACGGAGAATTCGGGAATTCCGGCGCGGGTTCTGATTTGTCCCAGAGAGTGAAATGGAGCAGCAAGATCCCACCCGAGCATGTTAATACGTATTCTCTGCAGAATTTTATCCAGGGAGATGAGTGGATTAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

540

Amino Acids

58.52

Weight (kDa)

6.52

Isoelectric Point (pI)

34.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	38 - 181	4.6e-18	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	230 - 534	3.2e-120	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1336
AatII	GACGTC	1 cut(s)	336
Acc36I	ACCTGC	2 cut(s)	983, 1336
Acc65I	GGTACC	1 cut(s)	351
AccB1I	GGYRCC	2 cut(s)	351, 823
AccBSI	CCGCTC	1 cut(s)	607
AccII	CGCG	9 cut(s)	152, 254, 317, 393, 425, 536, 752, 764, 1508
AclWI	GGATC	2 cut(s)	109, 1543
AcsI	RAATTY	6 cut(s)	357, 709, 1061, 1490, 1498, 1585
AcvI	CACGTG	1 cut(s)	407
AcyI	GRCGYC	2 cut(s)	333, 1002
AfaI	GTAC	4 cut(s)	353, 464, 557, 1385
AfiI	CCNNNNNNNGG	4 cut(s)	348, 632, 1220, 1454
AflIII	ACRYGT	1 cut(s)	150
AgeI	ACCGGT	1 cut(s)	371
AgsI	TTSAA	3 cut(s)	895, 1289, 1471
AhdI	GACNNNNNGTC	1 cut(s)	448
AjiI	CACGTC	1 cut(s)	691
AjnI	CCWGG	5 cut(s)	165, 276, 648, 1173, 1593
AjuI	GAANNNNNNNTTGG	2 cut(s)	1091, 1123
AluBI	AGCT	4 cut(s)	121, 175, 1207, 1421
AluI	AGCT	4 cut(s)	121, 175, 1207, 1421
Alw21I	GWGCWC	2 cut(s)	1124, 1353
Alw26I	GTCTC	4 cut(s)	200, 235, 806, 977
Alw44I	GTGCAC	2 cut(s)	1120, 1349
AlwI	GGATC	2 cut(s)	109, 1543
Ama87I	CYCGRG	6 cut(s)	203, 1214, 1262, 1387, 1408, 1556
AoxI	GGCC	6 cut(s)	3, 132, 241, 590, 901, 1370
ApaLI	GTGCAC	2 cut(s)	1120, 1349
ApeKI	GCWGC	1 cut(s)	1542
ApoI	RAATTY	6 cut(s)	357, 709, 1061, 1490, 1498, 1585
AsiGI	ACCGGT	1 cut(s)	371
Asp718I	GGTACC	1 cut(s)	351
AspLEI	GCGC	4 cut(s)	256, 538, 754, 1508
AspS9I	GGNCC	5 cut(s)	265, 590, 642, 901, 995
AsuC2I	CCSGG	5 cut(s)	349, 886, 909, 1263, 1264
AsuHPI	GGTGA	4 cut(s)	358, 754, 869, 962
AsuNHI	GCTAGC	1 cut(s)	121
AvaI	CYCGRG	6 cut(s)	203, 1214, 1262, 1387, 1408, 1556
AvaII	GGWCC	3 cut(s)	265, 642, 995
BaeGI	GKGCMC	3 cut(s)	828, 1124, 1353
BanI	GGYRCC	2 cut(s)	351, 823
BarI	GAAGNNNNNNTAC	2 cut(s)	548, 580
BbrPI	CACGTG	1 cut(s)	407
Bbv12I	GWGCWC	2 cut(s)	1124, 1353
BbvI	GCAGC	1 cut(s)	1554
BccI	CCATC	8 cut(s)	104, 475, 859, 947, 1133, 1175, 1193, 1448
BceAI	ACGGC	3 cut(s)	206, 256, 1357
BcgI	CGANNNNNNTGC	4 cut(s)	520, 554, 1214, 1248
BciT130I	CCWGG	5 cut(s)	167, 278, 650, 1175, 1595
BclI	TGATCA	1 cut(s)	1299
BcnI	CCSGG	5 cut(s)	349, 886, 909, 1263, 1264
BcoDI	GTCTC	4 cut(s)	200, 235, 806, 977
BfaI	CTAG	3 cut(s)	122, 830, 1200
BfmI	CTRYAG	1 cut(s)	1580
BfuAI	ACCTGC	2 cut(s)	983, 1336
BglI	GCCNNNNNGGC	1 cut(s)	131
BisI	GCNGC	6 cut(s)	315, 423, 593, 750, 1343, 1543
BlsI	GCNGC	6 cut(s)	316, 424, 594, 751, 1344, 1544
BmcAI	AGTACT	1 cut(s)	1385
Bme18I	GGWCC	3 cut(s)	265, 642, 995
BmeRI	GACNNNNNGTC	1 cut(s)	448
BmeT110I	CYCGRG	6 cut(s)	203, 1214, 1262, 1387, 1408, 1556
BmgBI	CACGTC	1 cut(s)	691
BmgT120I	GGNCC	5 cut(s)	265, 590, 642, 901, 995
BmiI	GGNNCC	6 cut(s)	353, 591, 624, 643, 825, 997
BmsI	GCATC	1 cut(s)	1434
BmtI	GCTAGC	1 cut(s)	125
BpuEI	CTTGAG	2 cut(s)	230, 443
BpuMI	CCSGG	5 cut(s)	349, 886, 909, 1263, 1264
BsaAI	YACGTR	2 cut(s)	407, 1572
BsaHI	GRCGYC	2 cut(s)	333, 1002
BsaI	GGTCTC	3 cut(s)	235, 806, 977
BsaWI	WCCGGW	1 cut(s)	371
BsaXI	ACNNNNNCTCC	4 cut(s)	83, 113, 1148, 1178
Bsc4I	CCNNNNNNNGG	4 cut(s)	348, 632, 1220, 1454
Bse118I	RCCGGY	2 cut(s)	371, 625
Bse3DI	GCAATG	1 cut(s)	1141
BseBI	CCWGG	5 cut(s)	167, 278, 650, 1175, 1595
BseGI	GGATG	5 cut(s)	870, 1125, 1185, 1449, 1459
BseLI	CCNNNNNNNGG	4 cut(s)	348, 632, 1220, 1454
BseMI	GCAATG	1 cut(s)	1141
BseRI	GAGGAG	3 cut(s)	17, 64, 824
BseSI	GKGCMC	3 cut(s)	828, 1124, 1353
BseXI	GCAGC	1 cut(s)	1554
BseYI	CCCAGC	1 cut(s)	797
BsgI	GTGCAG	2 cut(s)	752, 1165
Bsh1236I	CGCG	9 cut(s)	152, 254, 317, 393, 425, 536, 752, 764, 1508
BshFI	GGCC	6 cut(s)	5, 134, 243, 592, 903, 1372
BshNI	GGYRCC	2 cut(s)	351, 823
BshTI	ACCGGT	1 cut(s)	371
BsiHKAI	GWGCWC	2 cut(s)	1124, 1353
BsiHKCI	CYCGRG	6 cut(s)	203, 1214, 1262, 1387, 1408, 1556
BsiSI	CCGG	8 cut(s)	349, 372, 626, 755, 885, 909, 1263, 1503
BsiWI	CGTACG	1 cut(s)	555
BslFI	GGGAC	3 cut(s)	623, 981, 1507
BslI	CCNNNNNNNGG	4 cut(s)	348, 632, 1220, 1454
BsmAI	GTCTC	4 cut(s)	200, 235, 806, 977
BsmFI	GGGAC	3 cut(s)	623, 981, 1507
BsmI	GAATGC	1 cut(s)	751
BsnI	GGCC	6 cut(s)	5, 134, 243, 592, 903, 1372
Bso31I	GGTCTC	3 cut(s)	235, 806, 977
BsoBI	CYCGRG	6 cut(s)	203, 1214, 1262, 1387, 1408, 1556
Bsp1286I	GDGCHC	3 cut(s)	828, 1124, 1353
Bsp143I	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
Bsp19I	CCATGG	1 cut(s)	1246
BspANI	GGCC	6 cut(s)	5, 134, 243, 592, 903, 1372
BspFNI	CGCG	9 cut(s)	152, 254, 317, 393, 425, 536, 752, 764, 1508
BspLI	GGNNCC	6 cut(s)	353, 591, 624, 643, 825, 997
BspMAI	CTGCAG	1 cut(s)	1584
BspMI	ACCTGC	2 cut(s)	983, 1336
BspOI	GCTAGC	1 cut(s)	125
BspPI	GGATC	2 cut(s)	109, 1543
BspT107I	GGYRCC	2 cut(s)	351, 823
BspTNI	GGTCTC	3 cut(s)	235, 806, 977
BsrBI	CCGCTC	1 cut(s)	607
BsrDI	GCAATG	1 cut(s)	1141
BsrFI	RCCGGY	2 cut(s)	371, 625
BssAI	RCCGGY	2 cut(s)	371, 625
BssMI	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
BssNI	GRCGYC	2 cut(s)	333, 1002
BssT1I	CCWWGG	3 cut(s)	1129, 1246, 1448
Bst2UI	CCWGG	5 cut(s)	167, 278, 650, 1175, 1595
Bst4CI	ACNGT	7 cut(s)	697, 730, 817, 877, 937, 979, 1396
Bst6I	CTCTTC	3 cut(s)	20, 47, 65
BstACI	GRCGYC	2 cut(s)	333, 1002
BstBAI	YACGTR	2 cut(s)	407, 1572
BstC8I	GCNNGC	6 cut(s)	123, 127, 154, 173, 905, 1340
BstDEI	CTNAG	1 cut(s)	579
BstDSI	CCRYGG	3 cut(s)	816, 1246, 1373
BstF5I	GGATG	5 cut(s)	870, 1125, 1185, 1449, 1459
BstFNI	CGCG	9 cut(s)	152, 254, 317, 393, 425, 536, 752, 764, 1508
BstHHI	GCGC	4 cut(s)	256, 538, 754, 1508
BstKTI	GATC	5 cut(s)	117, 519, 1028, 1302, 1551
BstMAI	GTCTC	4 cut(s)	200, 235, 806, 977
BstMBI	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
BstMWI	GCNNNNNNNGC	7 cut(s)	131, 158, 746, 1010, 1241, 1348, 1427
BstNI	CCWGG	5 cut(s)	167, 278, 650, 1175, 1595
BstNSI	RCATGY	1 cut(s)	1565
BstSFI	CTRYAG	1 cut(s)	1580
BstSLI	GKGCMC	3 cut(s)	828, 1124, 1353
BstSNI	TACGTA	1 cut(s)	1572
BstUI	CGCG	9 cut(s)	152, 254, 317, 393, 425, 536, 752, 764, 1508
BstV1I	GCAGC	1 cut(s)	1554
BstX2I	RGATCY	2 cut(s)	114, 1548
BstYI	RGATCY	2 cut(s)	114, 1548
BsuRI	GGCC	6 cut(s)	5, 134, 243, 592, 903, 1372
BtgI	CCRYGG	3 cut(s)	816, 1246, 1373
BtrI	CACGTC	1 cut(s)	691
BtsCI	GGATG	5 cut(s)	870, 1125, 1185, 1449, 1459
BtsI	GCAGTG	1 cut(s)	927
BtsIMutI	CAGTG	2 cut(s)	180, 927
BveI	ACCTGC	2 cut(s)	983, 1336
Cac8I	GCNNGC	6 cut(s)	123, 127, 154, 173, 905, 1340
CfoI	GCGC	4 cut(s)	256, 538, 754, 1508
Cfr10I	RCCGGY	2 cut(s)	371, 625
Cfr13I	GGNCC	5 cut(s)	265, 590, 642, 901, 995
Cfr9I	CCCGGG	1 cut(s)	1262
CseI	GACGC	2 cut(s)	158, 1010
Csp6I	GTAC	4 cut(s)	352, 463, 556, 1384
CspAI	ACCGGT	1 cut(s)	371
CviQI	GTAC	4 cut(s)	352, 463, 556, 1384
DdeI	CTNAG	1 cut(s)	579
DpnI	GATC	5 cut(s)	116, 518, 1027, 1301, 1550
DpnII	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
DraI	TTTAAA	1 cut(s)	681
DriI	GACNNNNNGTC	1 cut(s)	448
Eam1104I	CTCTTC	3 cut(s)	20, 47, 65
Eam1105I	GACNNNNNGTC	1 cut(s)	448
EarI	CTCTTC	3 cut(s)	20, 47, 65
EciI	GGCGGA	3 cut(s)	67, 651, 701
Eco105I	TACGTA	1 cut(s)	1572
Eco130I	CCWWGG	3 cut(s)	1129, 1246, 1448
Eco31I	GGTCTC	3 cut(s)	235, 806, 977
Eco47I	GGWCC	3 cut(s)	265, 642, 995
Eco72I	CACGTG	1 cut(s)	407
Eco88I	CYCGRG	6 cut(s)	203, 1214, 1262, 1387, 1408, 1556
EcoO109I	RGGNCCY	1 cut(s)	995
EcoRI	GAATTC	4 cut(s)	709, 1061, 1490, 1498
EcoRII	CCWGG	5 cut(s)	165, 276, 648, 1173, 1593
EcoT14I	CCWWGG	3 cut(s)	1129, 1246, 1448
ErhI	CCWWGG	3 cut(s)	1129, 1246, 1448
FalI	AAGNNNNNCTT	2 cut(s)	1346, 1378
FaqI	GGGAC	3 cut(s)	623, 981, 1507
FauI	CCCGC	5 cut(s)	283, 600, 656, 757, 1501
FbaI	TGATCA	1 cut(s)	1299
Fnu4HI	GCNGC	6 cut(s)	315, 423, 593, 750, 1343, 1543
FokI	GGATG	5 cut(s)	877, 1112, 1172, 1456, 1466
Fsp4HI	GCNGC	6 cut(s)	315, 423, 593, 750, 1343, 1543
FspBI	CTAG	3 cut(s)	122, 830, 1200
GlaI	GCGC	4 cut(s)	255, 537, 753, 1507
GluI	GCNGC	6 cut(s)	315, 423, 593, 750, 1343, 1543
GsaI	CCCAGC	1 cut(s)	801
HaeIII	GGCC	6 cut(s)	5, 134, 243, 592, 903, 1372
HapII	CCGG	8 cut(s)	349, 372, 626, 755, 885, 909, 1263, 1503
HgaI	GACGC	2 cut(s)	158, 1010
HhaI	GCGC	4 cut(s)	256, 538, 754, 1508
Hin1I	GRCGYC	2 cut(s)	333, 1002
Hin6I	GCGC	4 cut(s)	254, 536, 752, 1506
HinP1I	GCGC	4 cut(s)	254, 536, 752, 1506
HincII	GTYRAC	2 cut(s)	487, 1272
HindII	GTYRAC	2 cut(s)	487, 1272
HinfI	GANTC	3 cut(s)	281, 509, 667
HpaII	CCGG	8 cut(s)	349, 372, 626, 755, 885, 909, 1263, 1503
HphI	GGTGA	4 cut(s)	358, 754, 869, 962
Hpy166II	GTNNAC	6 cut(s)	268, 487, 805, 1122, 1272, 1351
Hpy188III	TCNNGA	9 cut(s)	203, 205, 247, 506, 781, 982, 998, 1076, 1495
Hpy8I	GTNNAC	6 cut(s)	268, 487, 805, 1122, 1272, 1351
Hpy99I	CGWCG	5 cut(s)	152, 335, 676, 941, 1004
HpyAV	CCTTC	3 cut(s)	20, 1213, 1372
HpyCH4III	ACNGT	7 cut(s)	697, 730, 817, 877, 937, 979, 1396
HpyCH4IV	ACGT	5 cut(s)	333, 406, 690, 843, 1571
HpyCH4V	TGCA	9 cut(s)	129, 702, 733, 1122, 1182, 1235, 1351, 1430, 1582
HpyF10VI	GCNNNNNNNGC	7 cut(s)	131, 158, 746, 1010, 1241, 1348, 1427
HpyF3I	CTNAG	1 cut(s)	579
HpySE526I	ACGT	5 cut(s)	333, 406, 690, 843, 1571
Hsp92I	GRCGYC	2 cut(s)	333, 1002
HspAI	GCGC	4 cut(s)	254, 536, 752, 1506
KpnI	GGTACC	1 cut(s)	355
Ksp22I	TGATCA	1 cut(s)	1299
Kzo9I	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
LmnI	GCTCC	3 cut(s)	622, 1335, 1539
Lsp1109I	GCAGC	1 cut(s)	1554
LweI	GCATC	1 cut(s)	1434
MaeI	CTAG	3 cut(s)	122, 830, 1200
MaeII	ACGT	5 cut(s)	333, 406, 690, 843, 1571
MaeIII	GTNAC	4 cut(s)	178, 691, 857, 1237
MalI	GATC	5 cut(s)	116, 518, 1027, 1301, 1550
MbiI	CCGCTC	1 cut(s)	607
MboI	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
MboII	GAAGA	7 cut(s)	7, 34, 52, 1141, 1162, 1263, 1325
MflI	RGATCY	2 cut(s)	114, 1548
MhlI	GDGCHC	3 cut(s)	828, 1124, 1353
MluCI	AATT	7 cut(s)	357, 709, 1061, 1106, 1490, 1498, 1585
MluI	ACGCGT	1 cut(s)	150
MlyI	GAGTC	2 cut(s)	275, 503
MmeI	TCCRAC	2 cut(s)	669, 919
MseI	TTAA	4 cut(s)	396, 680, 1566, 1611
MslI	CAYNNNNRTG	1 cut(s)	479
MspI	CCGG	8 cut(s)	349, 372, 626, 755, 885, 909, 1263, 1503
Mva1269I	GAATGC	1 cut(s)	751
MvaI	CCWGG	5 cut(s)	167, 278, 650, 1175, 1595
MvnI	CGCG	9 cut(s)	152, 254, 317, 393, 425, 536, 752, 764, 1508
MwoI	GCNNNNNNNGC	7 cut(s)	131, 158, 746, 1010, 1241, 1348, 1427
NciI	CCSGG	5 cut(s)	349, 886, 909, 1263, 1264
NcoI	CCATGG	1 cut(s)	1246
NdeII	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
NheI	GCTAGC	1 cut(s)	121
NlaIV	GGNNCC	6 cut(s)	353, 591, 624, 643, 825, 997
NmeAIII	GCCGAG	1 cut(s)	519
NmuCI	GTSAC	4 cut(s)	178, 691, 857, 1237
NspI	RCATGY	1 cut(s)	1565
PaeR7I	CTCGAG	3 cut(s)	203, 1387, 1408
PaqCI	CACCTGC	1 cut(s)	1336
PasI	CCCWGGG	1 cut(s)	166
PcsI	WCGNNNNNNNCGW	2 cut(s)	813, 1056
PctI	GAATGC	1 cut(s)	751
PfeI	GAWTC	1 cut(s)	667
Pfl23II	CGTACG	1 cut(s)	555
PflFI	GACNNNGTC	1 cut(s)	496
PfoI	TCCNGGA	2 cut(s)	276, 1173
PinAI	ACCGGT	1 cut(s)	371
PkrI	GCNGC	6 cut(s)	316, 424, 594, 751, 1344, 1544
PleI	GAGTC	2 cut(s)	275, 503
PmaCI	CACGTG	1 cut(s)	407
PmlI	CACGTG	1 cut(s)	407
PpsI	GAGTC	2 cut(s)	275, 503
Ppu21I	YACGTR	2 cut(s)	407, 1572
PpuMI	RGGWCCY	1 cut(s)	995
Psp5II	RGGWCCY	1 cut(s)	995
Psp6I	CCWGG	5 cut(s)	165, 276, 648, 1173, 1593
PspCI	CACGTG	1 cut(s)	407
PspFI	CCCAGC	1 cut(s)	797
PspGI	CCWGG	5 cut(s)	165, 276, 648, 1173, 1593
PspLI	CGTACG	1 cut(s)	555
PspN4I	GGNNCC	6 cut(s)	353, 591, 624, 643, 825, 997
PspPI	GGNCC	5 cut(s)	265, 590, 642, 901, 995
PspPPI	RGGWCCY	1 cut(s)	995
PspXI	VCTCGAGB	2 cut(s)	1387, 1408
PstI	CTGCAG	1 cut(s)	1584
PsuI	RGATCY	2 cut(s)	114, 1548
PsyI	GACNNNGTC	1 cut(s)	496
RsaI	GTAC	4 cut(s)	353, 464, 557, 1385
RsaNI	GTAC	4 cut(s)	352, 463, 556, 1384
RseI	CAYNNNNRTG	1 cut(s)	479
SaqAI	TTAA	4 cut(s)	396, 680, 1566, 1611
SatI	GCNGC	6 cut(s)	315, 423, 593, 750, 1343, 1543
Sau3AI	GATC	5 cut(s)	114, 516, 1025, 1299, 1548
Sau96I	GGNCC	5 cut(s)	265, 590, 642, 901, 995
ScaI	AGTACT	1 cut(s)	1385
SchI	GAGTC	2 cut(s)	275, 503
SduI	GDGCHC	3 cut(s)	828, 1124, 1353
SfaNI	GCATC	1 cut(s)	1434
SfcI	CTRYAG	1 cut(s)	1580
Sfr274I	CTCGAG	3 cut(s)	203, 1387, 1408
SgrAI	CRCCGGYG	1 cut(s)	371
SinI	GGWCC	3 cut(s)	265, 642, 995
SlaI	CTCGAG	3 cut(s)	203, 1387, 1408
SmaI	CCCGGG	1 cut(s)	1264
SmiMI	CAYNNNNRTG	1 cut(s)	479
SmlI	CTYRAG	5 cut(s)	203, 245, 458, 1387, 1408
SmoI	CTYRAG	5 cut(s)	203, 245, 458, 1387, 1408
SnaBI	TACGTA	1 cut(s)	1572
Sse9I	AATT	7 cut(s)	357, 709, 1061, 1106, 1490, 1498, 1585
SspMI	CTAG	3 cut(s)	122, 830, 1200
StyI	CCWWGG	3 cut(s)	1129, 1246, 1448
TaaI	ACNGT	7 cut(s)	697, 730, 817, 877, 937, 979, 1396
TaiI	ACGT	5 cut(s)	336, 409, 693, 846, 1574
TaqI	TCGA	7 cut(s)	204, 330, 507, 1050, 1209, 1388, 1409
TasI	AATT	7 cut(s)	357, 709, 1061, 1106, 1490, 1498, 1585
TatI	WGTACW	1 cut(s)	1383
TauI	GCSGC	5 cut(s)	317, 425, 595, 752, 1345
TfiI	GAWTC	1 cut(s)	667
Tru1I	TTAA	4 cut(s)	396, 680, 1566, 1611
Tru9I	TTAA	4 cut(s)	396, 680, 1566, 1611
TscAI	CASTG	2 cut(s)	187, 934
TseFI	GTSAC	4 cut(s)	178, 691, 857, 1237
TseI	GCWGC	1 cut(s)	1542
Tsp45I	GTSAC	4 cut(s)	178, 691, 857, 1237
TspDTI	ATGAA	6 cut(s)	203, 949, 1054, 1135, 1337, 1390
TspGWI	ACGGA	4 cut(s)	660, 1033, 1325, 1500
TspMI	CCCGGG	1 cut(s)	1262
TspRI	CASTG	2 cut(s)	187, 934
Tth111I	GACNNNGTC	1 cut(s)	496
VneI	GTGCAC	2 cut(s)	1120, 1349
VpaK11BI	GGWCC	3 cut(s)	265, 642, 995
XapI	RAATTY	6 cut(s)	357, 709, 1061, 1490, 1498, 1585
XceI	RCATGY	1 cut(s)	1565
XhoI	CTCGAG	3 cut(s)	203, 1387, 1408
XmaI	CCCGGG	1 cut(s)	1262
XspI	CTAG	3 cut(s)	122, 830, 1200
ZraI	GACGTC	1 cut(s)	334
ZrmI	AGTACT	1 cut(s)	1385

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.