Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Reverse (-)

8349749 .. 8351377

Gene Structure 1629 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g14710.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGGCATCCCGGTTTTTTCTCCTCACGCTCACCCTTACTCTCTCCCTCACCCTTTCATCCGCCACTCGCTTCCTCTCAGACTCCAAACTCGCCGCCTCATCTCCGATCGACCAAGCCTGCAAAGCCACCCGCTTCCCTGACACCTGCAAGGCCTCCTTGTCAAATAAAACCCATGACCTTCCCTCTGACCCTAGCCCTCTCCAAACTCTCCAGCTAGCCCTCAATGTCTCCTTCAACGGCCTCAAAACCGCCCAAAATATGGTGGCCGACATTCTCAACTCCGCCGCCGGCAGCGTGAACCGTACAACCGGCGCCAAAAATTGCATTATTAATCTACAAAACTCCGAGTACCGCATATTACTCTCCCAGGACGCCCTCAAAAATGGCCGGACCAAAACCGCACGCGCCGCCATGAGCTCCGCCCTGATCTACCAGTTTGACTGCTGGTACGCCTTCAAGTACGCCAACGACACCCAGTTGGTCATCTCCACCATGTCATTCATCAACGACTCCCTGACCGTCGACACCAGCAACGCCCTCAGCATGATCTTTTCGTATGAAAACTTTGGAAACGACACCAAGTCTTGGTCCCCGCCCAAAACCGAGCGTGACGGATTATGGCAGGTCACGCTCTCTAACAATTCGTCCTCAGACTATTCTGTACCAAAAGATCTGACGGTGAACGTGACTGTTTGTAAGGATGATAAAACTGACTGTTACCGGACCATACAGGCAGCGGTGGACGCGGCGCCGGAAAATTCCAACGAATTTTTTGTGATTAAGATAAATGCCGGGGTGTATGAAGAGATTGTTGGGATTCCTCTAGAGAAGAGGAATGTTGTGTTTCTAGGGGAAGGCATGGGCAAAACAGTAATTACAGGGTCTTTGTATGTGGGCCTGCCAGGAATTGGGACGTACAACTCCGCCACCGTGGCTGTTTACGGCGACGGCTTCATGGCCAGCGGCGTCACATTTGAGAACAAGGCCGGCCCCGACGCCAAGCAGGAGGTCCACCAGGCGGTGGCTTTCCGATCAGACAGTGATCGCTCTATCATCGAGGACTGTGAGTTCCTTGGGCACCAAGACACTCTCTACGCTCGTAGCAACCGCCAGCTCTACAAGTCATGCAGAATCCAAGGCAATGTCGACTTCATATTCGGAAATGCGGCTGCCATGTTCGACAACTGCACCATCCTCATCGAGTCTAGGGAAGTGACCCCAGAGAAAGGGGAGAAGAACGCCGTGACGGCTCACGGCAGGACAGACCCGGCGCAGGCGACGGGGTTCGTGTTCCGGAACTGCTTCATTAACGGGACGGAAGAGTACATGAAGTTGTACAAAAAGGATCCCAAAGTGCATTTGAACTACTTAGGGAGGCCATGGAAGGAGTTTTCGAGGACGGTGTTCTTGAATTGTAGCATGGAAGGTCTGGTTTCAAAGGAAGGGTGGATGCCTTGGAGTGACCAGTTTGCTTTGGCGACACTTTACTATGGGGAGTATGGGAACTCGGGACCGGGGGCAGATTTGGCCGGGAGGGTGAAGTGGAGTAGTGAGATTCCGGCCGAGCATGTTGATGCTTATTCCGTGCAGAATTTCGTTCAGGGAGATGAGTGGTTGGTTTACAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

543

Amino Acids

59.76

Weight (kDa)

5.38

Isoelectric Point (pI)

32.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	36 - 182	5e-18	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	228 - 536	2e-109	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	152
Acc36I	ACCTGC	2 cut(s)	152, 613
AccB1I	GGYRCC	3 cut(s)	311, 748, 1077
AccB7I	CCANNNNNTGG	4 cut(s)	259, 585, 908, 1021
AccI	GTMKAC	2 cut(s)	522, 1146
AccII	CGCG	2 cut(s)	405, 746
AccIII	TCCGGA	1 cut(s)	1293
AclWI	GGATC	2 cut(s)	1340, 1353
AcoI	YGGCCR	5 cut(s)	264, 385, 957, 1527, 1560
AcsI	RAATTY	3 cut(s)	757, 767, 1591
AcyI	GRCGYC	5 cut(s)	312, 372, 749, 966, 996
AfaI	GTAC	8 cut(s)	304, 350, 449, 461, 663, 917, 1325, 1337
AfiI	CCNNNNNNNGG	7 cut(s)	259, 585, 908, 1018, 1021, 1226, 1273
AgsI	TTSAA	5 cut(s)	235, 457, 1363, 1411, 1437
AhdI	GACNNNNNGTC	1 cut(s)	580
AjnI	CCWGG	3 cut(s)	366, 901, 1014
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
AloI	GAACNNNNNNTCC	2 cut(s)	1052, 1084
AluBI	AGCT	3 cut(s)	214, 417, 1114
AluI	AGCT	3 cut(s)	214, 417, 1114
Alw21I	GWGCWC	1 cut(s)	419
Alw26I	GTCTC	1 cut(s)	232
AlwI	GGATC	2 cut(s)	1340, 1353
Ama87I	CYCGRG	1 cut(s)	1507
Aor13HI	TCCGGA	1 cut(s)	1293
ApeKI	GCWGC	3 cut(s)	291, 734, 1169
ApoI	RAATTY	3 cut(s)	757, 767, 1591
ArsI	GACNNNNNNTTYG	2 cut(s)	1139, 1171
AseI	ATTAAT	1 cut(s)	330
Asp700I	GAANNNNTTC	1 cut(s)	1301
AspLEI	GCGC	4 cut(s)	314, 407, 751, 1273
AspS9I	GGNCC	7 cut(s)	390, 588, 723, 895, 989, 1009, 1511
AsuC2I	CCSGG	5 cut(s)	10, 793, 1268, 1515, 1531
AsuHPI	GGTGA	4 cut(s)	22, 40, 691, 1549
AsuNHI	GCTAGC	1 cut(s)	214
AvaI	CYCGRG	1 cut(s)	1507
AvaII	GGWCC	5 cut(s)	390, 588, 723, 1009, 1511
BaeGI	GKGCMC	1 cut(s)	1080
BalI	TGGCCA	1 cut(s)	959
BamHI	GGATCC	1 cut(s)	1345
BanI	GGYRCC	3 cut(s)	311, 748, 1077
BanII	GRGCYC	1 cut(s)	419
Bbv12I	GWGCWC	1 cut(s)	419
BbvCI	CCTCAGC	1 cut(s)	539
BbvI	GCAGC	3 cut(s)	303, 746, 1156
BccI	CCATC	1 cut(s)	1199
BceAI	ACGGC	6 cut(s)	253, 958, 964, 1226, 1263, 1270
BciT130I	CCWGG	3 cut(s)	368, 903, 1016
BcnI	CCSGG	5 cut(s)	10, 793, 1268, 1515, 1531
BcoDI	GTCTC	1 cut(s)	232
BfaI	CTAG	6 cut(s)	192, 215, 824, 848, 1206, 1627
BfoI	RGCGCY	2 cut(s)	315, 752
BfuAI	ACCTGC	2 cut(s)	152, 613
BglI	GCCNNNNNGGC	2 cut(s)	932, 1247
BglII	AGATCT	1 cut(s)	670
BisI	GCNGC	9 cut(s)	93, 285, 292, 408, 735, 747, 964, 1167, 1170
BlsI	GCNGC	9 cut(s)	94, 286, 293, 409, 736, 748, 965, 1168, 1171
Bme1390I	CCNGG	8 cut(s)	10, 368, 793, 903, 1016, 1268, 1515, 1531
Bme18I	GGWCC	5 cut(s)	390, 588, 723, 1009, 1511
BmeRI	GACNNNNNGTC	1 cut(s)	580
BmeT110I	CYCGRG	1 cut(s)	1507
BmgT120I	GGNCC	7 cut(s)	390, 588, 723, 895, 989, 1009, 1511
BmiI	GGNNCC	7 cut(s)	313, 590, 750, 991, 1079, 1347, 1512
BmrFI	CCNGG	8 cut(s)	10, 368, 793, 903, 1016, 1268, 1515, 1531
BmrI	ACTGGG	1 cut(s)	469
BmsI	GCATC	3 cut(s)	14, 1440, 1564
BmtI	GCTAGC	1 cut(s)	218
BmuI	ACTGGG	1 cut(s)	469
BpmI	CTGGAG	1 cut(s)	194
Bpu10I	CCTNAGC	1 cut(s)	539
BpuMI	CCSGG	5 cut(s)	10, 793, 1268, 1515, 1531
BsaHI	GRCGYC	5 cut(s)	312, 372, 749, 966, 996
BsaJI	CCNNGG	8 cut(s)	366, 792, 930, 1072, 1135, 1379, 1454, 1514
BsaWI	WCCGGW	2 cut(s)	720, 1293
Bsc4I	CCNNNNNNNGG	7 cut(s)	259, 585, 908, 1018, 1021, 1226, 1273
Bse118I	RCCGGY	3 cut(s)	287, 308, 986
Bse1I	ACTGG	3 cut(s)	433, 475, 1465
Bse3DI	GCAATG	1 cut(s)	1147
BseAI	TCCGGA	1 cut(s)	1293
BseBI	CCWGG	3 cut(s)	368, 903, 1016
BseDI	CCNNGG	8 cut(s)	366, 792, 930, 1072, 1135, 1379, 1454, 1514
BseGI	GGATG	5 cut(s)	5, 56, 706, 1191, 1455
BseLI	CCNNNNNNNGG	7 cut(s)	259, 585, 908, 1018, 1021, 1226, 1273
BseMI	GCAATG	1 cut(s)	1147
BseMII	CTCAG	3 cut(s)	90, 553, 663
BseNI	ACTGG	3 cut(s)	433, 475, 1465
BseRI	GAGGAG	1 cut(s)	11
BseSI	GKGCMC	1 cut(s)	1080
BseX3I	CGGCCG	1 cut(s)	1560
BseXI	GCAGC	3 cut(s)	303, 746, 1156
BsgI	GTGCAG	2 cut(s)	1171, 1607
Bsh1236I	CGCG	2 cut(s)	405, 746
Bsh1285I	CGRYCG	2 cut(s)	108, 1563
BshNI	GGYRCC	3 cut(s)	311, 748, 1077
BsiEI	CGRYCG	2 cut(s)	108, 1563
BsiHKAI	GWGCWC	1 cut(s)	419
BsiHKCI	CYCGRG	1 cut(s)	1507
BslFI	GGGAC	4 cut(s)	574, 925, 1327, 1524
BslI	CCNNNNNNNGG	7 cut(s)	259, 585, 908, 1018, 1021, 1226, 1273
BsmAI	GTCTC	1 cut(s)	232
BsmFI	GGGAC	4 cut(s)	574, 925, 1327, 1524
BsoBI	CYCGRG	1 cut(s)	1507
Bsp1286I	GDGCHC	2 cut(s)	419, 1080
Bsp13I	TCCGGA	1 cut(s)	1293
Bsp1407I	TGTACA	1 cut(s)	1335
Bsp143I	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
Bsp19I	CCATGG	1 cut(s)	1379
BspCNI	CTCAG	3 cut(s)	89, 552, 662
BspEI	TCCGGA	1 cut(s)	1293
BspFNI	CGCG	2 cut(s)	405, 746
BspLI	GGNNCC	7 cut(s)	313, 590, 750, 991, 1079, 1347, 1512
BspMI	ACCTGC	2 cut(s)	152, 613
BspOI	GCTAGC	1 cut(s)	218
BspPI	GGATC	2 cut(s)	1340, 1353
BspT107I	GGYRCC	3 cut(s)	311, 748, 1077
BsrDI	GCAATG	1 cut(s)	1147
BsrFI	RCCGGY	3 cut(s)	287, 308, 986
BsrGI	TGTACA	1 cut(s)	1335
BsrI	ACTGG	3 cut(s)	433, 475, 1465
BssAI	RCCGGY	3 cut(s)	287, 308, 986
BssECI	CCNNGG	8 cut(s)	366, 792, 930, 1072, 1135, 1379, 1454, 1514
BssMI	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
BssNI	GRCGYC	5 cut(s)	312, 372, 749, 966, 996
BssT1I	CCWWGG	4 cut(s)	1072, 1135, 1379, 1454
Bst2UI	CCWGG	3 cut(s)	368, 903, 1016
Bst6I	CTCTTC	3 cut(s)	798, 824, 1314
BstACI	GRCGYC	5 cut(s)	312, 372, 749, 966, 996
BstAUI	TGTACA	1 cut(s)	1335
BstC8I	GCNNGC	9 cut(s)	118, 216, 289, 403, 899, 961, 988, 1112, 1275
BstDEI	CTNAG	4 cut(s)	76, 539, 649, 1369
BstDSI	CCRYGG	2 cut(s)	930, 1379
BstF5I	GGATG	5 cut(s)	5, 56, 706, 1191, 1455
BstFNI	CGCG	2 cut(s)	405, 746
BstH2I	RGCGCY	2 cut(s)	315, 752
BstHHI	GCGC	4 cut(s)	314, 407, 751, 1273
BstKTI	GATC	7 cut(s)	108, 429, 549, 673, 1034, 1045, 1348
BstMAI	GTCTC	1 cut(s)	232
BstMBI	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
BstMCI	CGRYCG	2 cut(s)	108, 1563
BstMWI	GCNNNNNNNGC	7 cut(s)	122, 407, 628, 743, 932, 1247, 1526
BstNI	CCWGG	3 cut(s)	368, 903, 1016
BstNSI	RCATGY	1 cut(s)	1571
BstSCI	CCNGG	8 cut(s)	8, 366, 791, 901, 1014, 1266, 1513, 1529
BstSLI	GKGCMC	1 cut(s)	1080
BstUI	CGCG	2 cut(s)	405, 746
BstV1I	GCAGC	3 cut(s)	303, 746, 1156
BstX2I	RGATCY	2 cut(s)	670, 1345
BstYI	RGATCY	2 cut(s)	670, 1345
BstZI	CGGCCG	1 cut(s)	1560
BtgI	CCRYGG	2 cut(s)	930, 1379
BtsCI	GGATG	5 cut(s)	5, 56, 706, 1191, 1455
BtsIMutI	CAGTG	1 cut(s)	1045
BveI	ACCTGC	2 cut(s)	152, 613
Cac8I	GCNNGC	9 cut(s)	118, 216, 289, 403, 899, 961, 988, 1112, 1275
CfoI	GCGC	4 cut(s)	314, 407, 751, 1273
Cfr10I	RCCGGY	3 cut(s)	287, 308, 986
Cfr13I	GGNCC	7 cut(s)	390, 588, 723, 895, 989, 1009, 1511
CseI	GACGC	4 cut(s)	380, 752, 955, 1004
Csp6I	GTAC	8 cut(s)	303, 349, 448, 460, 662, 916, 1324, 1336
CviQI	GTAC	8 cut(s)	303, 349, 448, 460, 662, 916, 1324, 1336
DdeI	CTNAG	4 cut(s)	76, 539, 649, 1369
DinI	GGCGCC	2 cut(s)	313, 750
DpnI	GATC	7 cut(s)	107, 428, 548, 672, 1033, 1044, 1347
DpnII	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
DriI	GACNNNNNGTC	1 cut(s)	580
EaeI	YGGCCR	5 cut(s)	264, 385, 957, 1527, 1560
EagI	CGGCCG	1 cut(s)	1560
Eam1104I	CTCTTC	3 cut(s)	798, 824, 1314
Eam1105I	GACNNNNNGTC	1 cut(s)	580
EarI	CTCTTC	3 cut(s)	798, 824, 1314
EciI	GGCGGA	4 cut(s)	49, 271, 409, 913
Ecl136II	GAGCTC	1 cut(s)	417
EclXI	CGGCCG	1 cut(s)	1560
Eco130I	CCWWGG	4 cut(s)	1072, 1135, 1379, 1454
Eco147I	AGGCCT	1 cut(s)	152
Eco24I	GRGCYC	1 cut(s)	419
Eco47I	GGWCC	5 cut(s)	390, 588, 723, 1009, 1511
Eco52I	CGGCCG	1 cut(s)	1560
Eco53kI	GAGCTC	1 cut(s)	417
Eco88I	CYCGRG	1 cut(s)	1507
EcoICRI	GAGCTC	1 cut(s)	417
EcoRII	CCWGG	3 cut(s)	366, 901, 1014
EcoT14I	CCWWGG	4 cut(s)	1072, 1135, 1379, 1454
EcoT38I	GRGCYC	1 cut(s)	419
EgeI	GGCGCC	2 cut(s)	313, 750
EheI	GGCGCC	2 cut(s)	313, 750
ErhI	CCWWGG	4 cut(s)	1072, 1135, 1379, 1454
FalI	AAGNNNNNCTT	2 cut(s)	140, 172
FaqI	GGGAC	4 cut(s)	574, 925, 1327, 1524
FauI	CCCGC	2 cut(s)	137, 600
FblI	GTMKAC	2 cut(s)	522, 1146
Fnu4HI	GCNGC	9 cut(s)	93, 285, 292, 408, 735, 747, 964, 1167, 1170
FokI	GGATG	4 cut(s)	43, 713, 1178, 1462
FriOI	GRGCYC	1 cut(s)	419
FseI	GGCCGGCC	1 cut(s)	990
Fsp4HI	GCNGC	9 cut(s)	93, 285, 292, 408, 735, 747, 964, 1167, 1170
FspBI	CTAG	6 cut(s)	192, 215, 824, 848, 1206, 1627
GlaI	GCGC	4 cut(s)	313, 406, 750, 1272
GluI	GCNGC	9 cut(s)	93, 285, 292, 408, 735, 747, 964, 1167, 1170
GsuI	CTGGAG	1 cut(s)	194
HaeII	RGCGCY	2 cut(s)	315, 752
HgaI	GACGC	4 cut(s)	380, 752, 955, 1004
HhaI	GCGC	4 cut(s)	314, 407, 751, 1273
Hin1I	GRCGYC	5 cut(s)	312, 372, 749, 966, 996
Hin6I	GCGC	4 cut(s)	312, 405, 749, 1271
HinP1I	GCGC	4 cut(s)	312, 405, 749, 1271
HincII	GTYRAC	2 cut(s)	523, 1147
HindII	GTYRAC	2 cut(s)	523, 1147
HinfI	GANTC	6 cut(s)	80, 509, 817, 1131, 1202, 1555
HphI	GGTGA	4 cut(s)	22, 40, 691, 1549
Hpy166II	GTNNAC	8 cut(s)	298, 523, 682, 742, 940, 1012, 1147, 1621
Hpy188I	TCNGA	9 cut(s)	79, 105, 187, 346, 652, 675, 1031, 1036, 1160
Hpy188III	TCNNGA	4 cut(s)	824, 1294, 1408, 1509
Hpy8I	GTNNAC	8 cut(s)	298, 523, 682, 742, 940, 1012, 1147, 1621
Hpy99I	CGWCG	4 cut(s)	524, 950, 998, 1282
HpyAV	CCTTC	7 cut(s)	188, 241, 463, 848, 1378, 1418, 1436
HpyCH4IV	ACGT	2 cut(s)	684, 914
HpyCH4V	TGCA	7 cut(s)	120, 147, 324, 1128, 1188, 1357, 1588
HpyF10VI	GCNNNNNNNGC	7 cut(s)	122, 407, 628, 743, 932, 1247, 1526
HpyF3I	CTNAG	4 cut(s)	76, 539, 649, 1369
HpySE526I	ACGT	2 cut(s)	684, 914
Hsp92I	GRCGYC	5 cut(s)	312, 372, 749, 966, 996
HspAI	GCGC	4 cut(s)	312, 405, 749, 1271
KasI	GGCGCC	2 cut(s)	311, 748
Kpn2I	TCCGGA	1 cut(s)	1293
KroI	GCCGGC	2 cut(s)	287, 986
KroNI	GCCGGC	2 cut(s)	289, 988
Kzo9I	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
LmnI	GCTCC	1 cut(s)	422
Lsp1109I	GCAGC	3 cut(s)	303, 746, 1156
LweI	GCATC	3 cut(s)	14, 1440, 1564
MaeI	CTAG	6 cut(s)	192, 215, 824, 848, 1206, 1627
MaeII	ACGT	2 cut(s)	684, 914
MaeIII	GTNAC	8 cut(s)	608, 625, 685, 716, 967, 1213, 1243, 1460
MalI	GATC	7 cut(s)	107, 428, 548, 672, 1033, 1044, 1347
MboI	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
MboII	GAAGA	4 cut(s)	815, 841, 1246, 1331
MflI	RGATCY	2 cut(s)	670, 1345
MhlI	GDGCHC	2 cut(s)	419, 1080
MlsI	TGGCCA	1 cut(s)	959
MluCI	AATT	8 cut(s)	319, 640, 757, 767, 873, 906, 1411, 1591
MluNI	TGGCCA	1 cut(s)	959
Mly113I	GGCGCC	2 cut(s)	312, 749
MlyI	GAGTC	3 cut(s)	74, 503, 1211
MmeI	TCCRAC	1 cut(s)	786
Mox20I	TGGCCA	1 cut(s)	959
MroI	TCCGGA	1 cut(s)	1293
MroNI	GCCGGC	2 cut(s)	287, 986
MroXI	GAANNNNTTC	1 cut(s)	1301
MscI	TGGCCA	1 cut(s)	959
MseI	TTAA	3 cut(s)	330, 780, 1308
MslI	CAYNNNNRTG	1 cut(s)	1572
Msp20I	TGGCCA	1 cut(s)	959
MspA1I	CMGCKG	2 cut(s)	737, 963
MspR9I	CCNGG	8 cut(s)	10, 368, 793, 903, 1016, 1268, 1515, 1531
MvaI	CCWGG	3 cut(s)	368, 903, 1016
MvnI	CGCG	2 cut(s)	405, 746
MwoI	GCNNNNNNNGC	7 cut(s)	122, 407, 628, 743, 932, 1247, 1526
NaeI	GCCGGC	2 cut(s)	289, 988
NarI	GGCGCC	2 cut(s)	312, 749
NciI	CCSGG	5 cut(s)	10, 793, 1268, 1515, 1531
NcoI	CCATGG	1 cut(s)	1379
NdeII	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
NgoMIV	GCCGGC	2 cut(s)	287, 986
NheI	GCTAGC	1 cut(s)	214
NlaIV	GGNNCC	7 cut(s)	313, 590, 750, 991, 1079, 1347, 1512
NmeAIII	GCCGAG	1 cut(s)	1588
NmuCI	GTSAC	7 cut(s)	608, 625, 685, 967, 1213, 1243, 1460
NspI	RCATGY	1 cut(s)	1571
PaqCI	CACCTGC	1 cut(s)	152
PceI	AGGCCT	1 cut(s)	152
PdiI	GCCGGC	2 cut(s)	289, 988
PdmI	GAANNNNTTC	1 cut(s)	1301
PfeI	GAWTC	3 cut(s)	817, 1131, 1555
PflMI	CCANNNNNTGG	4 cut(s)	259, 585, 908, 1021
PkrI	GCNGC	9 cut(s)	94, 286, 293, 409, 736, 748, 965, 1168, 1171
Ple19I	CGATCG	1 cut(s)	108
PleI	GAGTC	3 cut(s)	74, 503, 1210
PluTI	GGCGCC	2 cut(s)	315, 752
PpsI	GAGTC	3 cut(s)	74, 503, 1210
PshBI	ATTAAT	1 cut(s)	330
Psp124BI	GAGCTC	1 cut(s)	419
Psp6I	CCWGG	3 cut(s)	366, 901, 1014
PspGI	CCWGG	3 cut(s)	366, 901, 1014
PspN4I	GGNNCC	7 cut(s)	313, 590, 750, 991, 1079, 1347, 1512
PspPI	GGNCC	7 cut(s)	390, 588, 723, 895, 989, 1009, 1511
PsuI	RGATCY	2 cut(s)	670, 1345
PvuI	CGATCG	1 cut(s)	108
RigI	GGCCGGCC	1 cut(s)	990
RsaI	GTAC	8 cut(s)	304, 350, 449, 461, 663, 917, 1325, 1337
RsaNI	GTAC	8 cut(s)	303, 349, 448, 460, 662, 916, 1324, 1336
RseI	CAYNNNNRTG	1 cut(s)	1572
SacI	GAGCTC	1 cut(s)	419
SalI	GTCGAC	2 cut(s)	521, 1145
SaqAI	TTAA	3 cut(s)	330, 780, 1308
SatI	GCNGC	9 cut(s)	93, 285, 292, 408, 735, 747, 964, 1167, 1170
Sau3AI	GATC	7 cut(s)	105, 426, 546, 670, 1031, 1042, 1345
Sau96I	GGNCC	7 cut(s)	390, 588, 723, 895, 989, 1009, 1511
SchI	GAGTC	3 cut(s)	74, 503, 1211
ScrFI	CCNGG	8 cut(s)	10, 368, 793, 903, 1016, 1268, 1515, 1531
SduI	GDGCHC	2 cut(s)	419, 1080
SfaNI	GCATC	3 cut(s)	14, 1440, 1564
SfoI	GGCGCC	2 cut(s)	313, 750
SinI	GGWCC	5 cut(s)	390, 588, 723, 1009, 1511
SmiMI	CAYNNNNRTG	1 cut(s)	1572
Sse9I	AATT	8 cut(s)	319, 640, 757, 767, 873, 906, 1411, 1591
SseBI	AGGCCT	1 cut(s)	152
SspDI	GGCGCC	2 cut(s)	311, 748
SspMI	CTAG	6 cut(s)	192, 215, 824, 848, 1206, 1627
SstI	GAGCTC	1 cut(s)	419
StuI	AGGCCT	1 cut(s)	152
StyD4I	CCNGG	8 cut(s)	8, 366, 791, 901, 1014, 1266, 1513, 1529
StyI	CCWWGG	4 cut(s)	1072, 1135, 1379, 1454
TaiI	ACGT	2 cut(s)	687, 917
TaqI	TCGA	7 cut(s)	108, 522, 1056, 1146, 1179, 1200, 1394
TasI	AATT	8 cut(s)	319, 640, 757, 767, 873, 906, 1411, 1591
TatI	WGTACW	2 cut(s)	1323, 1335
TauI	GCSGC	6 cut(s)	95, 287, 410, 749, 966, 1169
TfiI	GAWTC	3 cut(s)	817, 1131, 1555
Tru1I	TTAA	3 cut(s)	330, 780, 1308
Tru9I	TTAA	3 cut(s)	330, 780, 1308
TscAI	CASTG	1 cut(s)	1045
TseFI	GTSAC	7 cut(s)	608, 625, 685, 967, 1213, 1243, 1460
TseI	GCWGC	3 cut(s)	291, 734, 1169
Tsp45I	GTSAC	7 cut(s)	608, 625, 685, 967, 1213, 1243, 1460
TspDTI	ATGAA	8 cut(s)	45, 490, 573, 816, 943, 1141, 1294, 1343
TspGWI	ACGGA	3 cut(s)	627, 1331, 1573
TspRI	CASTG	1 cut(s)	1045
Van91I	CCANNNNNTGG	4 cut(s)	259, 585, 908, 1021
VpaK11BI	GGWCC	5 cut(s)	390, 588, 723, 1009, 1511
VspI	ATTAAT	1 cut(s)	330
XapI	RAATTY	3 cut(s)	757, 767, 1591
XbaI	TCTAGA	1 cut(s)	823
XceI	RCATGY	1 cut(s)	1571
XmiI	GTMKAC	2 cut(s)	522, 1146
XmnI	GAANNNNTTC	1 cut(s)	1301
XspI	CTAG	6 cut(s)	192, 215, 824, 848, 1206, 1627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.