Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Forward (+)

3238263 .. 3239912

Gene Structure 1650 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ16205

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1650 bp

ATGTCTACCCGCGTCTTTCTCCTCACACTCGCTCTCTGCATCTCTCTTTCCTCCGCCTCAGCCTCCGCCGCCCGCTTCCCCTCCATCCCCAAGCCCACCGCCACCGCGGCCGTATCTCCGATCCAACAAGCCTGCAAATCCACCCGCTTCCCTGACACATGTCGGGCCTCCCTGACCAAAAAAATCCATGACCTTCCCCCAAACCCTACCCCTCTCCAAACACTTCAACTAGCCCTCAATGTCTCCTCCGACGGCCTCAACACCGCCAAAAAGATGGTTTCCGACATTCTCAAAACCGCCGCCGGCAGCCAGAACCGCACAACCGCCGCCAAAAACTGCCTCGATTTTCTACAAAATTCCGAGTACCGTATTTCACTCACCAATGACGGCCTCTCAAGTGGCCGTACGAAGACCGCACGAGCCGCCATGAGCTCCGCCCTGATGTACCAGTACGACTGCTGGTCCGCCTTCAAGTACGCCAACGACACCCAGTTAGTCGTCTCCACCATGTCGTTCATTAACGACACGCTGACCGTGGACTCCAGCAACGCCCTTAGCATGATCTTCTCCTACGAAAACTTCGGAAACGACACCAAGTCCTGGACCCCGCCCAAGACCGAGCGAGACGGGTTCTGGGAACCTGTCACGCCCGGATCACATAAGTCCCCTCCAGAACTTTCGATACCGAAAAATCTGATGGCGGACGTGACTGTTTGCAAGGACAAAGGTGTTAAGTGTTACCGGACCATACAGGAGGCGGTGAACGCGGCGCCGGAAAACTCCGACAAGAAATTCGTGATTCAGATAAATGCCGGGGTGTATGAAGAGATTGTTAAGGTTCCTCTAGGGAAGAGAAATGTTGTGTTTTTAGGGCAAGGGATGGGCAAAACAGTAATTACAGGTTCTTTGTTTGTGGGCCAGCCGGGAATTTCTACCTACAACTCCGCCACCGTGGGCGTTTACGGCGACGGCTTCATGGCCAGAGACGTCACATTCCAAAACACGGCGGGCCCCGACGCCCAGCAGGAGGTTCACCAGGCGGTGGCTTTCCGATCAGACAGTGATCACTCCATCATAGAAAATTGTGAGTTCCTAGGGCACCAAGACACTCTCTACGCCCACGGCAACCGCCAGTTCTACAAGTCATGCAAAATTCAAGGCAATGTAGATTTTATATTTGGAAACTCGGCTGCCATTTTCGAGGGCTGCACCATCCTCATTGCGCCGAGGCAAGTAAACCCCGAAAAAGGCGAGAAGAATGCCGTGACGGCTCACGGCAGGACCGACCCGGCGCAGGCCACAGGGTTCGTGTTCCGGAACTGCTTCATAAACGGAACGGAAGAGTACATGAAGTTGTACAACATTAACCCCAAAGTGCACTTGAACTTCTTGGGGAGGCCGTGGAAGGAGTTTTCGAGGACGGTGTTCTTGAACTGTAGTATGGAAGCTCTGGTTTCGAAAGAAGGGTGGTTGCCTTGGAGTGAGGAGTTTGCTTTGGCGACGCTTTACTATGGGGAGTATGGGAACTCGGGACCGGGGGCGGATTTGGCCGGTAGGGTGAAGTGGAGTAGCCGGATTCCGGTACAGCATGTTGGTGCATATTCGGTGCAAAATTTTATTCAGGGAGATGAGTGGTTGGTTTCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

549

Amino Acids

60.13

Weight (kDa)

8.15

Isoelectric Point (pI)

31.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	41 - 187	5.5e-20	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	235 - 543	1e-113	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	990
AccB1I	GGYRCC	2 cut(s)	769, 1098
AccB7I	CCANNNNNTGG	2 cut(s)	600, 1042
AccI	GTMKAC	1 cut(s)	5
AccII	CGCG	3 cut(s)	12, 107, 767
AccIII	TCCGGA	1 cut(s)	1314
AclWI	GGATC	2 cut(s)	115, 661
AcoI	YGGCCR	4 cut(s)	108, 400, 978, 1548
AcsI	RAATTY	5 cut(s)	355, 791, 927, 1152, 1612
AcyI	GRCGYC	3 cut(s)	770, 987, 1017
AfaI	GTAC	8 cut(s)	365, 406, 446, 452, 476, 1346, 1358, 1584
AfiI	CCNNNNNNNGG	7 cut(s)	600, 1003, 1027, 1042, 1247, 1294, 1579
AflIII	ACRYGT	1 cut(s)	158
AgsI	TTSAA	5 cut(s)	227, 472, 1157, 1384, 1432
AhdI	GACNNNNNGTC	2 cut(s)	460, 595
AjiI	CACGTC	1 cut(s)	706
AjnI	CCWGG	2 cut(s)	599, 1035
AluBI	AGCT	2 cut(s)	432, 1448
AluI	AGCT	2 cut(s)	432, 1448
Alw21I	GWGCWC	2 cut(s)	434, 1380
Alw26I	GTCTC	4 cut(s)	247, 505, 618, 978
Alw44I	GTGCAC	1 cut(s)	1376
AlwI	GGATC	2 cut(s)	115, 661
Ama87I	CYCGRG	1 cut(s)	1528
Aor13HI	TCCGGA	1 cut(s)	1314
ApaI	GGGCCC	1 cut(s)	1013
ApaLI	GTGCAC	1 cut(s)	1376
ApeKI	GCWGC	3 cut(s)	306, 1190, 1206
ApoI	RAATTY	5 cut(s)	355, 791, 927, 1152, 1612
ArsI	GACNNNNNNTTYG	2 cut(s)	776, 808
Asp700I	GAANNNNTTC	1 cut(s)	1322
AspA2I	CCTAGG	1 cut(s)	1093
AspLEI	GCGC	3 cut(s)	772, 1225, 1294
AspS9I	GGNCC	9 cut(s)	165, 462, 603, 744, 916, 1009, 1010, 1281, 1532
AsuC2I	CCSGG	5 cut(s)	651, 814, 924, 1289, 1536
AsuHPI	GGTGA	4 cut(s)	370, 772, 1025, 1570
AsuII	TTCGAA	1 cut(s)	1457
AvaI	CYCGRG	1 cut(s)	1528
AvaII	GGWCC	5 cut(s)	462, 603, 744, 1281, 1532
AvrII	CCTAGG	1 cut(s)	1093
BaeGI	GKGCMC	3 cut(s)	1013, 1101, 1380
BalI	TGGCCA	1 cut(s)	980
BanI	GGYRCC	2 cut(s)	769, 1098
BanII	GRGCYC	2 cut(s)	434, 1013
BauI	CACGAG	1 cut(s)	417
BbsI	GAAGAC	1 cut(s)	416
Bbv12I	GWGCWC	2 cut(s)	434, 1380
BbvCI	CCTCAGC	1 cut(s)	58
BbvI	GCAGC	3 cut(s)	318, 1177, 1193
BccI	CCATC	6 cut(s)	92, 268, 691, 874, 1079, 1220
BcgI	CGANNNNNNTGC	2 cut(s)	1241, 1275
BciT130I	CCWGG	2 cut(s)	601, 1037
BclI	TGATCA	1 cut(s)	1063
BcnI	CCSGG	5 cut(s)	651, 814, 924, 1289, 1536
BcoDI	GTCTC	4 cut(s)	247, 505, 618, 978
BfaI	CTAG	4 cut(s)	230, 845, 1094, 1648
BfmI	CTRYAG	1 cut(s)	1435
BfoI	RGCGCY	1 cut(s)	773
BglI	GCCNNNNNGGC	2 cut(s)	107, 1268
BisI	GCNGC	9 cut(s)	69, 108, 300, 307, 327, 423, 768, 1191, 1207
BlnI	CCTAGG	1 cut(s)	1093
BlsI	GCNGC	9 cut(s)	70, 109, 301, 308, 328, 424, 769, 1192, 1208
Bme1390I	CCNGG	7 cut(s)	601, 651, 814, 924, 1037, 1289, 1536
Bme18I	GGWCC	5 cut(s)	462, 603, 744, 1281, 1532
BmeRI	GACNNNNNGTC	2 cut(s)	460, 595
BmeT110I	CYCGRG	1 cut(s)	1528
BmgBI	CACGTC	1 cut(s)	706
BmgT120I	GGNCC	9 cut(s)	165, 462, 603, 744, 916, 1009, 1010, 1281, 1532
BmiI	GGNNCC	8 cut(s)	605, 639, 771, 840, 1011, 1012, 1100, 1533
BmrFI	CCNGG	7 cut(s)	601, 651, 814, 924, 1037, 1289, 1536
BmrI	ACTGGG	1 cut(s)	484
BmsI	GCATC	1 cut(s)	48
BmuI	ACTGGG	1 cut(s)	484
BoxI	GACNNNNGTC	1 cut(s)	159
BpiI	GAAGAC	1 cut(s)	416
BpmI	CTGGAG	2 cut(s)	526, 654
Bpu10I	CCTNAGC	2 cut(s)	58, 554
Bpu14I	TTCGAA	1 cut(s)	1457
BpuEI	CTTGAG	1 cut(s)	379
BpuMI	CCSGG	5 cut(s)	651, 814, 924, 1289, 1536
BsaHI	GRCGYC	3 cut(s)	770, 987, 1017
BsaWI	WCCGGW	3 cut(s)	741, 1314, 1579
Bsc4I	CCNNNNNNNGG	7 cut(s)	600, 1003, 1027, 1042, 1247, 1294, 1579
Bse118I	RCCGGY	2 cut(s)	302, 1550
Bse1I	ACTGG	3 cut(s)	448, 490, 1132
Bse3DI	GCAATG	2 cut(s)	1168, 1218
BseAI	TCCGGA	1 cut(s)	1314
BseBI	CCWGG	2 cut(s)	601, 1037
BseGI	GGATG	3 cut(s)	84, 885, 1212
BseLI	CCNNNNNNNGG	7 cut(s)	600, 1003, 1027, 1042, 1247, 1294, 1579
BseMI	GCAATG	2 cut(s)	1168, 1218
BseMII	CTCAG	1 cut(s)	72
BseNI	ACTGG	3 cut(s)	448, 490, 1132
BseRI	GAGGAG	3 cut(s)	11, 235, 1499
BseSI	GKGCMC	3 cut(s)	1013, 1101, 1380
BseX3I	CGGCCG	1 cut(s)	108
BseXI	GCAGC	3 cut(s)	318, 1177, 1193
BseYI	CCCAGC	1 cut(s)	1020
BsgI	GTGCAG	1 cut(s)	1192
Bsh1236I	CGCG	3 cut(s)	12, 107, 767
Bsh1285I	CGRYCG	1 cut(s)	111
BshNI	GGYRCC	2 cut(s)	769, 1098
BsiEI	CGRYCG	1 cut(s)	111
BsiHKAI	GWGCWC	2 cut(s)	434, 1380
BsiHKCI	CYCGRG	1 cut(s)	1528
BsiWI	CGTACG	1 cut(s)	404
BslFI	GGGAC	2 cut(s)	649, 1545
BslI	CCNNNNNNNGG	7 cut(s)	600, 1003, 1027, 1042, 1247, 1294, 1579
BsmAI	GTCTC	4 cut(s)	247, 505, 618, 978
BsmBI	CGTCTC	3 cut(s)	505, 618, 978
BsmFI	GGGAC	2 cut(s)	649, 1545
BsmI	GAATGC	1 cut(s)	1264
BsoBI	CYCGRG	1 cut(s)	1528
Bsp119I	TTCGAA	1 cut(s)	1457
Bsp120I	GGGCCC	1 cut(s)	1009
Bsp1286I	GDGCHC	4 cut(s)	434, 1013, 1101, 1380
Bsp13I	TCCGGA	1 cut(s)	1314
Bsp1407I	TGTACA	1 cut(s)	1356
Bsp143I	GATC	5 cut(s)	120, 561, 653, 1052, 1063
BspCNI	CTCAG	1 cut(s)	71
BspEI	TCCGGA	1 cut(s)	1314
BspFNI	CGCG	3 cut(s)	12, 107, 767
BspLI	GGNNCC	8 cut(s)	605, 639, 771, 840, 1011, 1012, 1100, 1533
BspPI	GGATC	2 cut(s)	115, 661
BspT104I	TTCGAA	1 cut(s)	1457
BspT107I	GGYRCC	2 cut(s)	769, 1098
BsrDI	GCAATG	2 cut(s)	1168, 1218
BsrFI	RCCGGY	2 cut(s)	302, 1550
BsrGI	TGTACA	1 cut(s)	1356
BsrI	ACTGG	3 cut(s)	448, 490, 1132
BssAI	RCCGGY	2 cut(s)	302, 1550
BssMI	GATC	5 cut(s)	120, 561, 653, 1052, 1063
BssNI	GRCGYC	3 cut(s)	770, 987, 1017
BssSI	CACGAG	1 cut(s)	417
BssT1I	CCWWGG	2 cut(s)	1093, 1475
Bst2BI	CACGAG	1 cut(s)	417
Bst2UI	CCWGG	2 cut(s)	601, 1037
Bst4CI	ACNGT	8 cut(s)	368, 535, 712, 892, 952, 1061, 1423, 1436
Bst6I	CTCTTC	3 cut(s)	819, 845, 1335
BstACI	GRCGYC	3 cut(s)	770, 987, 1017
BstAUI	TGTACA	1 cut(s)	1356
BstBI	TTCGAA	1 cut(s)	1457
BstC8I	GCNNGC	6 cut(s)	73, 133, 304, 920, 1009, 1296
BstDEI	CTNAG	2 cut(s)	58, 554
BstDSI	CCRYGG	5 cut(s)	105, 534, 951, 1120, 1400
BstF5I	GGATG	3 cut(s)	84, 885, 1212
BstFNI	CGCG	3 cut(s)	12, 107, 767
BstH2I	RGCGCY	1 cut(s)	773
BstHHI	GCGC	3 cut(s)	772, 1225, 1294
BstKTI	GATC	5 cut(s)	123, 564, 656, 1055, 1066
BstMAI	GTCTC	4 cut(s)	247, 505, 618, 978
BstMBI	GATC	5 cut(s)	120, 561, 653, 1052, 1063
BstMCI	CGRYCG	1 cut(s)	111
BstMWI	GCNNNNNNNGC	8 cut(s)	68, 107, 315, 422, 764, 963, 1268, 1547
BstNI	CCWGG	2 cut(s)	601, 1037
BstNSI	RCATGY	2 cut(s)	162, 1592
BstPAI	GACNNNNGTC	1 cut(s)	159
BstSCI	CCNGG	7 cut(s)	599, 649, 812, 922, 1035, 1287, 1534
BstSFI	CTRYAG	1 cut(s)	1435
BstSLI	GKGCMC	3 cut(s)	1013, 1101, 1380
BstUI	CGCG	3 cut(s)	12, 107, 767
BstV1I	GCAGC	3 cut(s)	318, 1177, 1193
BstV2I	GAAGAC	1 cut(s)	416
BstXI	CCANNNNNNTGG	1 cut(s)	274
BstZI	CGGCCG	1 cut(s)	108
BtgI	CCRYGG	5 cut(s)	105, 534, 951, 1120, 1400
BtrI	CACGTC	1 cut(s)	706
BtsCI	GGATG	3 cut(s)	84, 885, 1212
BtsIMutI	CAGTG	1 cut(s)	1066
Cac8I	GCNNGC	6 cut(s)	73, 133, 304, 920, 1009, 1296
CfoI	GCGC	3 cut(s)	772, 1225, 1294
Cfr10I	RCCGGY	2 cut(s)	302, 1550
Cfr13I	GGNCC	9 cut(s)	165, 462, 603, 744, 916, 1009, 1010, 1281, 1532
Cfr42I	CCGCGG	1 cut(s)	108
CseI	GACGC	2 cut(s)	1025, 1510
Csp6I	GTAC	8 cut(s)	364, 405, 445, 451, 475, 1345, 1357, 1583
CviAII	CATG	9 cut(s)	159, 188, 427, 508, 559, 976, 1146, 1348, 1589
CviQI	GTAC	8 cut(s)	364, 405, 445, 451, 475, 1345, 1357, 1583
DdeI	CTNAG	2 cut(s)	58, 554
DinI	GGCGCC	1 cut(s)	771
DpnI	GATC	5 cut(s)	122, 563, 655, 1054, 1065
DpnII	GATC	5 cut(s)	120, 561, 653, 1052, 1063
DriI	GACNNNNNGTC	2 cut(s)	460, 595
EaeI	YGGCCR	4 cut(s)	108, 400, 978, 1548
EagI	CGGCCG	1 cut(s)	108
Eam1104I	CTCTTC	3 cut(s)	819, 845, 1335
Eam1105I	GACNNNNNGTC	2 cut(s)	460, 595
EarI	CTCTTC	3 cut(s)	819, 845, 1335
EciI	GGCGGA	7 cut(s)	43, 55, 424, 454, 716, 934, 1556
Ecl136II	GAGCTC	1 cut(s)	432
EclXI	CGGCCG	1 cut(s)	108
Eco130I	CCWWGG	2 cut(s)	1093, 1475
Eco24I	GRGCYC	2 cut(s)	434, 1013
Eco47I	GGWCC	5 cut(s)	462, 603, 744, 1281, 1532
Eco52I	CGGCCG	1 cut(s)	108
Eco53kI	GAGCTC	1 cut(s)	432
Eco88I	CYCGRG	1 cut(s)	1528
EcoICRI	GAGCTC	1 cut(s)	432
EcoO109I	RGGNCCY	1 cut(s)	1010
EcoRII	CCWGG	2 cut(s)	599, 1035
EcoT14I	CCWWGG	2 cut(s)	1093, 1475
EcoT38I	GRGCYC	2 cut(s)	434, 1013
EgeI	GGCGCC	1 cut(s)	771
EheI	GGCGCC	1 cut(s)	771
ErhI	CCWWGG	2 cut(s)	1093, 1475
Esp3I	CGTCTC	3 cut(s)	505, 618, 978
FaeI	CATG	9 cut(s)	162, 191, 430, 511, 562, 979, 1149, 1351, 1592
FaqI	GGGAC	2 cut(s)	649, 1545
FatI	CATG	9 cut(s)	158, 187, 426, 507, 558, 975, 1145, 1347, 1588
FauI	CCCGC	5 cut(s)	17, 80, 152, 615, 1000
FbaI	TGATCA	1 cut(s)	1063
FblI	GTMKAC	1 cut(s)	5
Fnu4HI	GCNGC	9 cut(s)	69, 108, 300, 307, 327, 423, 768, 1191, 1207
FokI	GGATG	3 cut(s)	71, 892, 1199
FriOI	GRGCYC	2 cut(s)	434, 1013
Fsp4HI	GCNGC	9 cut(s)	69, 108, 300, 307, 327, 423, 768, 1191, 1207
FspBI	CTAG	4 cut(s)	230, 845, 1094, 1648
GlaI	GCGC	3 cut(s)	771, 1224, 1293
GluI	GCNGC	9 cut(s)	69, 108, 300, 307, 327, 423, 768, 1191, 1207
GsaI	CCCAGC	1 cut(s)	1024
GsuI	CTGGAG	2 cut(s)	526, 654
HaeII	RGCGCY	1 cut(s)	773
HgaI	GACGC	2 cut(s)	1025, 1510
HhaI	GCGC	3 cut(s)	772, 1225, 1294
Hin1I	GRCGYC	3 cut(s)	770, 987, 1017
Hin1II	CATG	9 cut(s)	162, 191, 430, 511, 562, 979, 1149, 1351, 1592
Hin6I	GCGC	3 cut(s)	770, 1223, 1292
HinP1I	GCGC	3 cut(s)	770, 1223, 1292
HinfI	GANTC	3 cut(s)	539, 799, 1576
HphI	GGTGA	4 cut(s)	370, 772, 1025, 1570
Hpy166II	GTNNAC	7 cut(s)	6, 538, 763, 961, 1033, 1237, 1378
Hpy188III	TCNNGA	5 cut(s)	671, 796, 1315, 1429, 1530
Hpy8I	GTNNAC	7 cut(s)	6, 538, 763, 961, 1033, 1237, 1378
Hpy99I	CGWCG	4 cut(s)	254, 971, 1019, 1504
HpyAV	CCTTC	4 cut(s)	203, 478, 1399, 1457
HpyCH4III	ACNGT	8 cut(s)	368, 535, 712, 892, 952, 1061, 1423, 1436
HpyCH4IV	ACGT	2 cut(s)	705, 987
HpyCH4V	TGCA	8 cut(s)	39, 135, 717, 1149, 1209, 1378, 1598, 1609
HpyF10VI	GCNNNNNNNGC	8 cut(s)	68, 107, 315, 422, 764, 963, 1268, 1547
HpyF3I	CTNAG	2 cut(s)	58, 554
HpySE526I	ACGT	2 cut(s)	705, 987
Hsp92I	GRCGYC	3 cut(s)	770, 987, 1017
Hsp92II	CATG	9 cut(s)	162, 191, 430, 511, 562, 979, 1149, 1351, 1592
HspAI	GCGC	3 cut(s)	770, 1223, 1292
KasI	GGCGCC	1 cut(s)	769
Kpn2I	TCCGGA	1 cut(s)	1314
KroI	GCCGGC	1 cut(s)	302
KroNI	GCCGGC	1 cut(s)	304
Ksp22I	TGATCA	1 cut(s)	1063
KspI	CCGCGG	1 cut(s)	108
Kzo9I	GATC	5 cut(s)	120, 561, 653, 1052, 1063
LmnI	GCTCC	1 cut(s)	437
Lsp1109I	GCAGC	3 cut(s)	318, 1177, 1193
LweI	GCATC	1 cut(s)	48
MaeI	CTAG	4 cut(s)	230, 845, 1094, 1648
MaeII	ACGT	2 cut(s)	705, 987
MaeIII	GTNAC	5 cut(s)	643, 706, 737, 988, 1264
MalI	GATC	5 cut(s)	122, 563, 655, 1054, 1065
MboI	GATC	5 cut(s)	120, 561, 653, 1052, 1063
MboII	GAAGA	6 cut(s)	421, 556, 836, 862, 1267, 1352
MhlI	GDGCHC	4 cut(s)	434, 1013, 1101, 1380
MlsI	TGGCCA	1 cut(s)	980
MluCI	AATT	7 cut(s)	355, 791, 894, 927, 1081, 1152, 1612
MluNI	TGGCCA	1 cut(s)	980
Mly113I	GGCGCC	1 cut(s)	770
MlyI	GAGTC	1 cut(s)	533
MmeI	TCCRAC	4 cut(s)	148, 273, 306, 807
Mox20I	TGGCCA	1 cut(s)	980
MroI	TCCGGA	1 cut(s)	1314
MroNI	GCCGGC	1 cut(s)	302
MroXI	GAANNNNTTC	1 cut(s)	1322
MscI	TGGCCA	1 cut(s)	980
MseI	TTAA	4 cut(s)	519, 732, 834, 1365
MslI	CAYNNNNRTG	1 cut(s)	1593
Msp20I	TGGCCA	1 cut(s)	980
MspA1I	CMGCKG	1 cut(s)	107
MspR9I	CCNGG	7 cut(s)	601, 651, 814, 924, 1037, 1289, 1536
Mva1269I	GAATGC	1 cut(s)	1264
MvaI	CCWGG	2 cut(s)	601, 1037
MvnI	CGCG	3 cut(s)	12, 107, 767
MwoI	GCNNNNNNNGC	8 cut(s)	68, 107, 315, 422, 764, 963, 1268, 1547
NaeI	GCCGGC	1 cut(s)	304
NarI	GGCGCC	1 cut(s)	770
NciI	CCSGG	5 cut(s)	651, 814, 924, 1289, 1536
NdeII	GATC	5 cut(s)	120, 561, 653, 1052, 1063
NgoMIV	GCCGGC	1 cut(s)	302
NlaIII	CATG	9 cut(s)	162, 191, 430, 511, 562, 979, 1149, 1351, 1592
NlaIV	GGNNCC	8 cut(s)	605, 639, 771, 840, 1011, 1012, 1100, 1533
NmeAIII	GCCGAG	2 cut(s)	1166, 1251
NmuCI	GTSAC	4 cut(s)	643, 706, 988, 1264
NspI	RCATGY	2 cut(s)	162, 1592
NspV	TTCGAA	1 cut(s)	1457
PciI	ACATGT	1 cut(s)	158
PcsI	WCGNNNNNNNCGW	1 cut(s)	1281
PctI	GAATGC	1 cut(s)	1264
PdiI	GCCGGC	1 cut(s)	304
PdmI	GAANNNNTTC	1 cut(s)	1322
PfeI	GAWTC	2 cut(s)	799, 1576
Pfl23II	CGTACG	1 cut(s)	404
PflMI	CCANNNNNTGG	2 cut(s)	600, 1042
PfoI	TCCNGGA	1 cut(s)	599
PkrI	GCNGC	9 cut(s)	70, 109, 301, 308, 328, 424, 769, 1192, 1208
PleI	GAGTC	1 cut(s)	533
PluTI	GGCGCC	1 cut(s)	773
PpsI	GAGTC	1 cut(s)	533
PscI	ACATGT	1 cut(s)	158
PshAI	GACNNNNGTC	1 cut(s)	159
Psp124BI	GAGCTC	1 cut(s)	434
Psp6I	CCWGG	2 cut(s)	599, 1035
PspFI	CCCAGC	1 cut(s)	1020
PspGI	CCWGG	2 cut(s)	599, 1035
PspLI	CGTACG	1 cut(s)	404
PspN4I	GGNNCC	8 cut(s)	605, 639, 771, 840, 1011, 1012, 1100, 1533
PspOMI	GGGCCC	1 cut(s)	1009
PspPI	GGNCC	9 cut(s)	165, 462, 603, 744, 916, 1009, 1010, 1281, 1532
PsrI	GAACNNNNNNTAC	2 cut(s)	666, 698
RsaI	GTAC	8 cut(s)	365, 406, 446, 452, 476, 1346, 1358, 1584
RsaNI	GTAC	8 cut(s)	364, 405, 445, 451, 475, 1345, 1357, 1583
RseI	CAYNNNNRTG	1 cut(s)	1593
SacI	GAGCTC	1 cut(s)	434
SacII	CCGCGG	1 cut(s)	108
SaqAI	TTAA	4 cut(s)	519, 732, 834, 1365
SatI	GCNGC	9 cut(s)	69, 108, 300, 307, 327, 423, 768, 1191, 1207
Sau3AI	GATC	5 cut(s)	120, 561, 653, 1052, 1063
Sau96I	GGNCC	9 cut(s)	165, 462, 603, 744, 916, 1009, 1010, 1281, 1532
SchI	GAGTC	1 cut(s)	533
ScrFI	CCNGG	7 cut(s)	601, 651, 814, 924, 1037, 1289, 1536
SduI	GDGCHC	4 cut(s)	434, 1013, 1101, 1380
SfaNI	GCATC	1 cut(s)	48
SfcI	CTRYAG	1 cut(s)	1435
SfoI	GGCGCC	1 cut(s)	771
Sfr303I	CCGCGG	1 cut(s)	108
SfuI	TTCGAA	1 cut(s)	1457
SgrBI	CCGCGG	1 cut(s)	108
SinI	GGWCC	5 cut(s)	462, 603, 744, 1281, 1532
SmiMI	CAYNNNNRTG	1 cut(s)	1593
SmlI	CTYRAG	1 cut(s)	394
SmoI	CTYRAG	1 cut(s)	394
Sse9I	AATT	7 cut(s)	355, 791, 894, 927, 1081, 1152, 1612
SspDI	GGCGCC	1 cut(s)	769
SspMI	CTAG	4 cut(s)	230, 845, 1094, 1648
SstI	GAGCTC	1 cut(s)	434
StyD4I	CCNGG	7 cut(s)	599, 649, 812, 922, 1035, 1287, 1534
StyI	CCWWGG	2 cut(s)	1093, 1475
TaaI	ACNGT	8 cut(s)	368, 535, 712, 892, 952, 1061, 1423, 1436
TaiI	ACGT	2 cut(s)	708, 990
TaqI	TCGA	5 cut(s)	342, 680, 1200, 1415, 1457
TaqII	GACCGA	2 cut(s)	632, 1298
TasI	AATT	7 cut(s)	355, 791, 894, 927, 1081, 1152, 1612
TatI	WGTACW	2 cut(s)	1344, 1356
TauI	GCSGC	6 cut(s)	71, 110, 302, 329, 425, 770
TfiI	GAWTC	2 cut(s)	799, 1576
Tru1I	TTAA	4 cut(s)	519, 732, 834, 1365
Tru9I	TTAA	4 cut(s)	519, 732, 834, 1365
TscAI	CASTG	1 cut(s)	1066
TseFI	GTSAC	4 cut(s)	643, 706, 988, 1264
TseI	GCWGC	3 cut(s)	306, 1190, 1206
Tsp45I	GTSAC	4 cut(s)	643, 706, 988, 1264
TspDTI	ATGAA	5 cut(s)	505, 837, 964, 1315, 1364
TspGWI	ACGGA	2 cut(s)	1347, 1352
TspRI	CASTG	1 cut(s)	1066
Van91I	CCANNNNNTGG	2 cut(s)	600, 1042
VneI	GTGCAC	1 cut(s)	1376
VpaK11BI	GGWCC	5 cut(s)	462, 603, 744, 1281, 1532
XapI	RAATTY	5 cut(s)	355, 791, 927, 1152, 1612
XceI	RCATGY	2 cut(s)	162, 1592
XmaJI	CCTAGG	1 cut(s)	1093
XmiI	GTMKAC	1 cut(s)	5
XmnI	GAANNNNTTC	1 cut(s)	1322
XspI	CTAG	4 cut(s)	230, 845, 1094, 1648
ZraI	GACGTC	1 cut(s)	988

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.