Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Forward (+)

3042766 .. 3044409

Gene Structure 1644 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G003760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGTCTACCCGCGTCTTTCTCCTCACACTCGCTCTCTGCATCTCTCTTTCCTCCGCCTCCGCCGCCCGCTTCCCCTCCATCCCCAAGCCCACCGCCACCGCCGCCGTATCTCCAATCCAACAAGCCTGCAAATCCACCCGCTTCCCCGACACATGTCGGGCCTCCCTGACCAAAAAAATCCATGACCTTCCCCCAAACCCTACCCCTCTCCAAACTCTTCAACTAGCCCTCAATGTCTCCTCCGACGGCCTCAACACCGCCAAAAAGATGGTTTCCGACATTCTCAAAACCGCCGCCGGCAGCCAGAACCGCACAACCGCCGCCAAAAACTGCCTCGATTTTCTACAAAATTCCGAGTACCGTATTTCACTCACCAATGACGGCCTCTCACGTGGCCGTACCAAGACCGCACGAGCCGCCATGAGCTCCGCCCTGATGTACCAGTACGACTGCTGGTCCGCCTTCAAGTACGCCAACGACACCCAGTTGGTCGTCTCCACCATGTCGTTCATTAACGACACGCTGACCGTGGACTCCAGCAACGCCCTTAGCATGATCTTCTCCTACGAAAACTTCGGAAACGACACCAAGTCCTGGACCCCGCCCAAGACCGAGCGAGACGGGTTATGGGAACCTGTCACGCCCGGATCACAAAAGTCCCCTCCAGAACTTTCGATACCGAAAAATCTGATGGCGGACGTGACTGTTTGCAAGGACAAAGGTGTTAAGTGTTACCGGACCATACAGGAGGCGGTGAACGCGGCGCCGGAAAACTCCGACAAGAAATTCGTGATTCAGATAAATGCCGGGGTGTATGAAGAGATTGTTAAGGTTCCTCTAGGGAAGAGAAATGTTGTGTTTTTAGGGCAAGGGATTGGCAAAACAGTAATTACAGGTTCTTTGTTTGTGGGCCAGCCGGGAATTTCGACCTACAACTCCGCCACCGTGGGCGTCACATTCCAAAACACGGCGGGCCCCGACGCCCAGCAGGAGGTTCACCAGGCGGTGGCTTTCCGATCAGACAGTGATCACTCCATCATAGAAAATTGTGAGTTCCTAGGGCACCAAGACACTCTCTACGCCCATGGCAACCGCCAGTTCTACAAGTCATGCAAAATTCAAGGCAATGTAGATTTTATATTTGGAAACTCGGCTGCCATTTTCGAGGGCTGCACCATCCTCATTGCGCCGAGGCAAGTAAATCCCGAAAAAGGCGAGAAGAATGCCGTGACGGCTCACGGCAGGACGGACCCGGCGCAGGCGACAGGGTTCGTGTTCTGGAACTGCTTCATAAACGGAACGGAAGAGTACATGAAGTTGTACAACATTAACCCCAAAGTGCACTTGAACTTCTTGGGGAGGCCGTGGAAGGAGTTTTCGAGGACGGTGTTCTTGAACTGTAGTATGGAAGCTCTGGTTTCGAAAGAAGGGTGGTTGCCTTGGAGTGAGGAGTTTGCTTTGGCGACGCTTTACTATGGGGAGTATGGGAACTCGGGACCGGGGTCCGATTTGGCCGGGAGGGTGAATTGGAGTAGCCGGATTCCGGTTGAGCATGTTGGTGCATATTCGGTGCAAAATTTTATTCAGGGAGATGAGTGGTTGGTTTCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

537

Amino Acids

58.9

Weight (kDa)

7.89

Isoelectric Point (pI)

31.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	39 - 185	4.1e-20	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	233 - 531	2.5e-102	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	763, 1062
AccB7I	CCANNNNNTGG	2 cut(s)	594, 1006
AccI	GTMKAC	1 cut(s)	5
AccII	CGCG	2 cut(s)	12, 761
AclWI	GGATC	1 cut(s)	655
AcoI	YGGCCR	2 cut(s)	394, 1512
AcsI	RAATTY	5 cut(s)	349, 785, 921, 1116, 1576
AcvI	CACGTG	1 cut(s)	392
AcyI	GRCGYC	3 cut(s)	764, 951, 981
AfaI	GTAC	7 cut(s)	359, 400, 440, 446, 470, 1310, 1322
AfiI	CCNNNNNNNGG	7 cut(s)	594, 967, 991, 1006, 1211, 1258, 1543
AflIII	ACRYGT	1 cut(s)	152
AgsI	TTSAA	5 cut(s)	221, 466, 1121, 1348, 1396
AhdI	GACNNNNNGTC	2 cut(s)	454, 589
AjiI	CACGTC	1 cut(s)	700
AjnI	CCWGG	2 cut(s)	593, 999
AluBI	AGCT	2 cut(s)	426, 1412
AluI	AGCT	2 cut(s)	426, 1412
Alw21I	GWGCWC	2 cut(s)	428, 1344
Alw26I	GTCTC	3 cut(s)	241, 499, 612
Alw44I	GTGCAC	1 cut(s)	1340
AlwI	GGATC	1 cut(s)	655
Ama87I	CYCGRG	1 cut(s)	1492
AoxI	GGCC	8 cut(s)	159, 247, 382, 394, 910, 973, 1361, 1512
ApaI	GGGCCC	1 cut(s)	977
ApaLI	GTGCAC	1 cut(s)	1340
ApeKI	GCWGC	3 cut(s)	300, 1154, 1170
ApoI	RAATTY	5 cut(s)	349, 785, 921, 1116, 1576
ArsI	GACNNNNNNTTYG	2 cut(s)	770, 802
Asp700I	GAANNNNTTC	1 cut(s)	1286
AspA2I	CCTAGG	1 cut(s)	1057
AspLEI	GCGC	3 cut(s)	766, 1189, 1258
AsuC2I	CCSGG	6 cut(s)	645, 808, 918, 1253, 1500, 1516
AsuHPI	GGTGA	4 cut(s)	364, 766, 989, 1534
AsuII	TTCGAA	1 cut(s)	1421
AvaI	CYCGRG	1 cut(s)	1492
AvaII	GGWCC	6 cut(s)	456, 597, 738, 1249, 1496, 1503
AvrII	CCTAGG	1 cut(s)	1057
BaeGI	GKGCMC	3 cut(s)	977, 1065, 1344
BanI	GGYRCC	2 cut(s)	763, 1062
BanII	GRGCYC	2 cut(s)	428, 977
BauI	CACGAG	1 cut(s)	411
BbrPI	CACGTG	1 cut(s)	392
Bbv12I	GWGCWC	2 cut(s)	428, 1344
BbvI	GCAGC	3 cut(s)	312, 1141, 1157
BccI	CCATC	5 cut(s)	86, 262, 685, 1043, 1184
BceAI	ACGGC	9 cut(s)	89, 262, 381, 397, 984, 1211, 1248, 1255, 1348
BcgI	CGANNNNNNTGC	2 cut(s)	1205, 1239
BciT130I	CCWGG	2 cut(s)	595, 1001
BclI	TGATCA	1 cut(s)	1027
BcnI	CCSGG	6 cut(s)	645, 808, 918, 1253, 1500, 1516
BcoDI	GTCTC	3 cut(s)	241, 499, 612
BfaI	CTAG	4 cut(s)	224, 839, 1058, 1612
BfmI	CTRYAG	1 cut(s)	1399
BfoI	RGCGCY	1 cut(s)	767
BglI	GCCNNNNNGGC	1 cut(s)	1232
BisI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1155, 1171
BlnI	CCTAGG	1 cut(s)	1057
BlsI	GCNGC	9 cut(s)	64, 103, 295, 302, 322, 418, 763, 1156, 1172
Bme1390I	CCNGG	8 cut(s)	595, 645, 808, 918, 1001, 1253, 1500, 1516
Bme18I	GGWCC	6 cut(s)	456, 597, 738, 1249, 1496, 1503
BmeRI	GACNNNNNGTC	2 cut(s)	454, 589
BmeT110I	CYCGRG	1 cut(s)	1492
BmgBI	CACGTC	1 cut(s)	700
BmrFI	CCNGG	8 cut(s)	595, 645, 808, 918, 1001, 1253, 1500, 1516
BmrI	ACTGGG	1 cut(s)	478
BmsI	GCATC	1 cut(s)	48
BmuI	ACTGGG	1 cut(s)	478
BoxI	GACNNNNGTC	2 cut(s)	153, 1501
BpmI	CTGGAG	2 cut(s)	520, 648
Bpu10I	CCTNAGC	1 cut(s)	548
Bpu14I	TTCGAA	1 cut(s)	1421
BpuMI	CCSGG	6 cut(s)	645, 808, 918, 1253, 1500, 1516
BsaAI	YACGTR	1 cut(s)	392
BsaHI	GRCGYC	3 cut(s)	764, 951, 981
BsaJI	CCNNGG	9 cut(s)	528, 807, 945, 1057, 1084, 1190, 1364, 1439, 1499
BsaWI	WCCGGW	2 cut(s)	735, 1543
Bsc4I	CCNNNNNNNGG	7 cut(s)	594, 967, 991, 1006, 1211, 1258, 1543
Bse118I	RCCGGY	1 cut(s)	296
Bse1I	ACTGG	3 cut(s)	442, 484, 1096
Bse3DI	GCAATG	2 cut(s)	1132, 1182
BseBI	CCWGG	2 cut(s)	595, 1001
BseDI	CCNNGG	9 cut(s)	528, 807, 945, 1057, 1084, 1190, 1364, 1439, 1499
BseGI	GGATG	2 cut(s)	78, 1176
BseLI	CCNNNNNNNGG	7 cut(s)	594, 967, 991, 1006, 1211, 1258, 1543
BseMI	GCAATG	2 cut(s)	1132, 1182
BseNI	ACTGG	3 cut(s)	442, 484, 1096
BseRI	GAGGAG	3 cut(s)	11, 229, 1463
BseSI	GKGCMC	3 cut(s)	977, 1065, 1344
BseXI	GCAGC	3 cut(s)	312, 1141, 1157
BseYI	CCCAGC	1 cut(s)	984
BsgI	GTGCAG	1 cut(s)	1156
Bsh1236I	CGCG	2 cut(s)	12, 761
BshFI	GGCC	8 cut(s)	161, 249, 384, 396, 912, 975, 1363, 1514
BshNI	GGYRCC	2 cut(s)	763, 1062
BsiHKAI	GWGCWC	2 cut(s)	428, 1344
BsiHKCI	CYCGRG	1 cut(s)	1492
BslFI	GGGAC	2 cut(s)	643, 1509
BslI	CCNNNNNNNGG	7 cut(s)	594, 967, 991, 1006, 1211, 1258, 1543
BsmAI	GTCTC	3 cut(s)	241, 499, 612
BsmBI	CGTCTC	2 cut(s)	499, 612
BsmFI	GGGAC	2 cut(s)	643, 1509
BsmI	GAATGC	1 cut(s)	1228
BsnI	GGCC	8 cut(s)	161, 249, 384, 396, 912, 975, 1363, 1514
BsoBI	CYCGRG	1 cut(s)	1492
Bsp119I	TTCGAA	1 cut(s)	1421
Bsp120I	GGGCCC	1 cut(s)	973
Bsp1286I	GDGCHC	4 cut(s)	428, 977, 1065, 1344
Bsp1407I	TGTACA	1 cut(s)	1320
Bsp143I	GATC	4 cut(s)	555, 647, 1016, 1027
Bsp19I	CCATGG	1 cut(s)	1084
BspANI	GGCC	8 cut(s)	161, 249, 384, 396, 912, 975, 1363, 1514
BspFNI	CGCG	2 cut(s)	12, 761
BspPI	GGATC	1 cut(s)	655
BspT104I	TTCGAA	1 cut(s)	1421
BspT107I	GGYRCC	2 cut(s)	763, 1062
BsrDI	GCAATG	2 cut(s)	1132, 1182
BsrFI	RCCGGY	1 cut(s)	296
BsrGI	TGTACA	1 cut(s)	1320
BsrI	ACTGG	3 cut(s)	442, 484, 1096
BssAI	RCCGGY	1 cut(s)	296
BssECI	CCNNGG	9 cut(s)	528, 807, 945, 1057, 1084, 1190, 1364, 1439, 1499
BssMI	GATC	4 cut(s)	555, 647, 1016, 1027
BssNI	GRCGYC	3 cut(s)	764, 951, 981
BssSI	CACGAG	1 cut(s)	411
BssT1I	CCWWGG	3 cut(s)	1057, 1084, 1439
Bst2BI	CACGAG	1 cut(s)	411
Bst2UI	CCWGG	2 cut(s)	595, 1001
Bst4CI	ACNGT	8 cut(s)	362, 529, 706, 886, 946, 1025, 1387, 1400
Bst6I	CTCTTC	4 cut(s)	222, 813, 839, 1299
BstACI	GRCGYC	3 cut(s)	764, 951, 981
BstAUI	TGTACA	1 cut(s)	1320
BstBAI	YACGTR	1 cut(s)	392
BstBI	TTCGAA	1 cut(s)	1421
BstC8I	GCNNGC	6 cut(s)	67, 127, 298, 914, 973, 1260
BstDEI	CTNAG	1 cut(s)	548
BstDSI	CCRYGG	4 cut(s)	528, 945, 1084, 1364
BstF5I	GGATG	2 cut(s)	78, 1176
BstFNI	CGCG	2 cut(s)	12, 761
BstH2I	RGCGCY	1 cut(s)	767
BstHHI	GCGC	3 cut(s)	766, 1189, 1258
BstKTI	GATC	4 cut(s)	558, 650, 1019, 1030
BstMAI	GTCTC	3 cut(s)	241, 499, 612
BstMBI	GATC	4 cut(s)	555, 647, 1016, 1027
BstMWI	GCNNNNNNNGC	6 cut(s)	62, 101, 309, 416, 758, 1232
BstNI	CCWGG	2 cut(s)	595, 1001
BstNSI	RCATGY	2 cut(s)	156, 1556
BstPAI	GACNNNNGTC	2 cut(s)	153, 1501
BstSCI	CCNGG	8 cut(s)	593, 643, 806, 916, 999, 1251, 1498, 1514
BstSFI	CTRYAG	1 cut(s)	1399
BstSLI	GKGCMC	3 cut(s)	977, 1065, 1344
BstUI	CGCG	2 cut(s)	12, 761
BstV1I	GCAGC	3 cut(s)	312, 1141, 1157
BstXI	CCANNNNNNTGG	1 cut(s)	268
BsuRI	GGCC	8 cut(s)	161, 249, 384, 396, 912, 975, 1363, 1514
BtgI	CCRYGG	4 cut(s)	528, 945, 1084, 1364
BtrI	CACGTC	1 cut(s)	700
BtsCI	GGATG	2 cut(s)	78, 1176
BtsIMutI	CAGTG	1 cut(s)	1030
Cac8I	GCNNGC	6 cut(s)	67, 127, 298, 914, 973, 1260
CfoI	GCGC	3 cut(s)	766, 1189, 1258
Cfr10I	RCCGGY	1 cut(s)	296
CseI	GACGC	3 cut(s)	940, 989, 1474
Csp6I	GTAC	7 cut(s)	358, 399, 439, 445, 469, 1309, 1321
CviAII	CATG	9 cut(s)	153, 182, 421, 502, 553, 1085, 1110, 1312, 1553
CviQI	GTAC	7 cut(s)	358, 399, 439, 445, 469, 1309, 1321
DdeI	CTNAG	1 cut(s)	548
DinI	GGCGCC	1 cut(s)	765
DpnI	GATC	4 cut(s)	557, 649, 1018, 1029
DpnII	GATC	4 cut(s)	555, 647, 1016, 1027
DriI	GACNNNNNGTC	2 cut(s)	454, 589
EaeI	YGGCCR	2 cut(s)	394, 1512
Eam1104I	CTCTTC	4 cut(s)	222, 813, 839, 1299
Eam1105I	GACNNNNNGTC	2 cut(s)	454, 589
EarI	CTCTTC	4 cut(s)	222, 813, 839, 1299
EciI	GGCGGA	6 cut(s)	43, 49, 418, 448, 710, 928
Ecl136II	GAGCTC	1 cut(s)	426
Eco130I	CCWWGG	3 cut(s)	1057, 1084, 1439
Eco24I	GRGCYC	2 cut(s)	428, 977
Eco47I	GGWCC	6 cut(s)	456, 597, 738, 1249, 1496, 1503
Eco53kI	GAGCTC	1 cut(s)	426
Eco72I	CACGTG	1 cut(s)	392
Eco88I	CYCGRG	1 cut(s)	1492
EcoICRI	GAGCTC	1 cut(s)	426
EcoO109I	RGGNCCY	1 cut(s)	974
EcoRII	CCWGG	2 cut(s)	593, 999
EcoT14I	CCWWGG	3 cut(s)	1057, 1084, 1439
EcoT38I	GRGCYC	2 cut(s)	428, 977
EgeI	GGCGCC	1 cut(s)	765
EheI	GGCGCC	1 cut(s)	765
ErhI	CCWWGG	3 cut(s)	1057, 1084, 1439
Esp3I	CGTCTC	2 cut(s)	499, 612
FaeI	CATG	9 cut(s)	156, 185, 424, 505, 556, 1088, 1113, 1315, 1556
FaqI	GGGAC	2 cut(s)	643, 1509
FatI	CATG	9 cut(s)	152, 181, 420, 501, 552, 1084, 1109, 1311, 1552
FauI	CCCGC	5 cut(s)	17, 74, 146, 609, 964
FbaI	TGATCA	1 cut(s)	1027
FblI	GTMKAC	1 cut(s)	5
Fnu4HI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1155, 1171
FokI	GGATG	2 cut(s)	65, 1163
FriOI	GRGCYC	2 cut(s)	428, 977
Fsp4HI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1155, 1171
FspBI	CTAG	4 cut(s)	224, 839, 1058, 1612
GlaI	GCGC	3 cut(s)	765, 1188, 1257
GluI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1155, 1171
GsaI	CCCAGC	1 cut(s)	988
GsuI	CTGGAG	2 cut(s)	520, 648
HaeII	RGCGCY	1 cut(s)	767
HaeIII	GGCC	8 cut(s)	161, 249, 384, 396, 912, 975, 1363, 1514
HgaI	GACGC	3 cut(s)	940, 989, 1474
HhaI	GCGC	3 cut(s)	766, 1189, 1258
Hin1I	GRCGYC	3 cut(s)	764, 951, 981
Hin1II	CATG	9 cut(s)	156, 185, 424, 505, 556, 1088, 1113, 1315, 1556
Hin6I	GCGC	3 cut(s)	764, 1187, 1256
HinP1I	GCGC	3 cut(s)	764, 1187, 1256
HinfI	GANTC	3 cut(s)	533, 793, 1540
HphI	GGTGA	4 cut(s)	364, 766, 989, 1534
Hpy166II	GTNNAC	5 cut(s)	6, 532, 757, 997, 1342
Hpy188III	TCNNGA	6 cut(s)	665, 790, 1205, 1279, 1393, 1494
Hpy8I	GTNNAC	5 cut(s)	6, 532, 757, 997, 1342
Hpy99I	CGWCG	3 cut(s)	248, 983, 1468
HpyAV	CCTTC	4 cut(s)	197, 472, 1363, 1421
HpyCH4III	ACNGT	8 cut(s)	362, 529, 706, 886, 946, 1025, 1387, 1400
HpyCH4IV	ACGT	2 cut(s)	391, 699
HpyCH4V	TGCA	8 cut(s)	39, 129, 711, 1113, 1173, 1342, 1562, 1573
HpyF10VI	GCNNNNNNNGC	6 cut(s)	62, 101, 309, 416, 758, 1232
HpyF3I	CTNAG	1 cut(s)	548
HpySE526I	ACGT	2 cut(s)	391, 699
Hsp92I	GRCGYC	3 cut(s)	764, 951, 981
Hsp92II	CATG	9 cut(s)	156, 185, 424, 505, 556, 1088, 1113, 1315, 1556
HspAI	GCGC	3 cut(s)	764, 1187, 1256
KasI	GGCGCC	1 cut(s)	763
KroI	GCCGGC	1 cut(s)	296
KroNI	GCCGGC	1 cut(s)	298
Ksp22I	TGATCA	1 cut(s)	1027
Kzo9I	GATC	4 cut(s)	555, 647, 1016, 1027
LmnI	GCTCC	1 cut(s)	431
Lsp1109I	GCAGC	3 cut(s)	312, 1141, 1157
LweI	GCATC	1 cut(s)	48
MaeI	CTAG	4 cut(s)	224, 839, 1058, 1612
MaeII	ACGT	2 cut(s)	391, 699
MaeIII	GTNAC	5 cut(s)	637, 700, 731, 952, 1228
MalI	GATC	4 cut(s)	557, 649, 1018, 1029
MboI	GATC	4 cut(s)	555, 647, 1016, 1027
MboII	GAAGA	6 cut(s)	209, 550, 830, 856, 1231, 1316
MhlI	GDGCHC	4 cut(s)	428, 977, 1065, 1344
MluCI	AATT	8 cut(s)	349, 785, 888, 921, 1045, 1116, 1525, 1576
Mly113I	GGCGCC	1 cut(s)	764
MlyI	GAGTC	1 cut(s)	527
MmeI	TCCRAC	4 cut(s)	142, 267, 300, 801
MroNI	GCCGGC	1 cut(s)	296
MroXI	GAANNNNTTC	1 cut(s)	1286
MseI	TTAA	4 cut(s)	513, 726, 828, 1329
MslI	CAYNNNNRTG	1 cut(s)	1557
MspR9I	CCNGG	8 cut(s)	595, 645, 808, 918, 1001, 1253, 1500, 1516
Mva1269I	GAATGC	1 cut(s)	1228
MvaI	CCWGG	2 cut(s)	595, 1001
MvnI	CGCG	2 cut(s)	12, 761
MwoI	GCNNNNNNNGC	6 cut(s)	62, 101, 309, 416, 758, 1232
NaeI	GCCGGC	1 cut(s)	298
NarI	GGCGCC	1 cut(s)	764
NciI	CCSGG	6 cut(s)	645, 808, 918, 1253, 1500, 1516
NcoI	CCATGG	1 cut(s)	1084
NdeII	GATC	4 cut(s)	555, 647, 1016, 1027
NgoMIV	GCCGGC	1 cut(s)	296
NlaIII	CATG	9 cut(s)	156, 185, 424, 505, 556, 1088, 1113, 1315, 1556
NmeAIII	GCCGAG	2 cut(s)	1130, 1215
NmuCI	GTSAC	4 cut(s)	637, 700, 952, 1228
NspI	RCATGY	2 cut(s)	156, 1556
NspV	TTCGAA	1 cut(s)	1421
PciI	ACATGT	1 cut(s)	152
PctI	GAATGC	1 cut(s)	1228
PdiI	GCCGGC	1 cut(s)	298
PdmI	GAANNNNTTC	1 cut(s)	1286
PfeI	GAWTC	2 cut(s)	793, 1540
PflMI	CCANNNNNTGG	2 cut(s)	594, 1006
PfoI	TCCNGGA	1 cut(s)	593
PkrI	GCNGC	9 cut(s)	64, 103, 295, 302, 322, 418, 763, 1156, 1172
PleI	GAGTC	1 cut(s)	527
PluTI	GGCGCC	1 cut(s)	767
PmaCI	CACGTG	1 cut(s)	392
PmlI	CACGTG	1 cut(s)	392
PpsI	GAGTC	1 cut(s)	527
Ppu21I	YACGTR	1 cut(s)	392
PscI	ACATGT	1 cut(s)	152
PshAI	GACNNNNGTC	2 cut(s)	153, 1501
Psp124BI	GAGCTC	1 cut(s)	428
Psp6I	CCWGG	2 cut(s)	593, 999
PspCI	CACGTG	1 cut(s)	392
PspFI	CCCAGC	1 cut(s)	984
PspGI	CCWGG	2 cut(s)	593, 999
PspOMI	GGGCCC	1 cut(s)	973
PsrI	GAACNNNNNNTAC	2 cut(s)	660, 692
RsaI	GTAC	7 cut(s)	359, 400, 440, 446, 470, 1310, 1322
RsaNI	GTAC	7 cut(s)	358, 399, 439, 445, 469, 1309, 1321
RseI	CAYNNNNRTG	1 cut(s)	1557
SacI	GAGCTC	1 cut(s)	428
SaqAI	TTAA	4 cut(s)	513, 726, 828, 1329
SatI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1155, 1171
Sau3AI	GATC	4 cut(s)	555, 647, 1016, 1027
SchI	GAGTC	1 cut(s)	527
ScrFI	CCNGG	8 cut(s)	595, 645, 808, 918, 1001, 1253, 1500, 1516
SduI	GDGCHC	4 cut(s)	428, 977, 1065, 1344
SfaNI	GCATC	1 cut(s)	48
SfcI	CTRYAG	1 cut(s)	1399
SfoI	GGCGCC	1 cut(s)	765
SfuI	TTCGAA	1 cut(s)	1421
SinI	GGWCC	6 cut(s)	456, 597, 738, 1249, 1496, 1503
SmiMI	CAYNNNNRTG	1 cut(s)	1557
Sse9I	AATT	8 cut(s)	349, 785, 888, 921, 1045, 1116, 1525, 1576
SspDI	GGCGCC	1 cut(s)	763
SspMI	CTAG	4 cut(s)	224, 839, 1058, 1612
SstI	GAGCTC	1 cut(s)	428
StyD4I	CCNGG	8 cut(s)	593, 643, 806, 916, 999, 1251, 1498, 1514
StyI	CCWWGG	3 cut(s)	1057, 1084, 1439
TaaI	ACNGT	8 cut(s)	362, 529, 706, 886, 946, 1025, 1387, 1400
TaiI	ACGT	2 cut(s)	394, 702
TaqI	TCGA	6 cut(s)	336, 674, 926, 1164, 1379, 1421
TaqII	GACCGA	1 cut(s)	626
TasI	AATT	8 cut(s)	349, 785, 888, 921, 1045, 1116, 1525, 1576
TatI	WGTACW	2 cut(s)	1308, 1320
TauI	GCSGC	6 cut(s)	65, 104, 296, 323, 419, 764
TfiI	GAWTC	2 cut(s)	793, 1540
Tru1I	TTAA	4 cut(s)	513, 726, 828, 1329
Tru9I	TTAA	4 cut(s)	513, 726, 828, 1329
TscAI	CASTG	1 cut(s)	1030
TseFI	GTSAC	4 cut(s)	637, 700, 952, 1228
TseI	GCWGC	3 cut(s)	300, 1154, 1170
Tsp45I	GTSAC	4 cut(s)	637, 700, 952, 1228
TspDTI	ATGAA	4 cut(s)	499, 831, 1279, 1328
TspGWI	ACGGA	3 cut(s)	1262, 1311, 1316
TspRI	CASTG	1 cut(s)	1030
Van91I	CCANNNNNTGG	2 cut(s)	594, 1006
VneI	GTGCAC	1 cut(s)	1340
VpaK11BI	GGWCC	6 cut(s)	456, 597, 738, 1249, 1496, 1503
XapI	RAATTY	5 cut(s)	349, 785, 921, 1116, 1576
XceI	RCATGY	2 cut(s)	156, 1556
XmaJI	CCTAGG	1 cut(s)	1057
XmiI	GTMKAC	1 cut(s)	5
XmnI	GAANNNNTTC	1 cut(s)	1286
XspI	CTAG	4 cut(s)	224, 839, 1058, 1612

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.