Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Reverse (-)

33621179 .. 33624514

Gene Structure 3336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG295600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGACCACCCACCACAGAAAACCCATAAAACCCAGCTTCGCCTCACTCTCCAAACCATGTTCCTCCCTATCCAAACCCTCCAAATTCCTCTTCATCTCCATGGCCTCCTTCGTCTTCCTCTCTCTCCTAACCCTCACTCTCTTCCTCTCCCTCTCCTCCGCCGCCCGCCACCGCCACCCTTCCGCCACCGCCGCCGCCGTCACTCAGATCCAACTCGCCTGCAAAGCCACCCGCTTCCCCGACACGTGTCAATCCTCCCTCGCCCAGCTCCCTGACCTTCCTCCGAACCCGACCCCAATCGAGACCATCGGGCTAGCCATCCGGGTCTCCTCCGATGGGCTCAAGTCGGCCCAGAAAATGGTGACGGACATTCTTGAAGCGTCCAAGGGGAACCAGAACCGCACCCTCGCCGCGAATAACTGCCTCGAGGTTCTGGACAACTCCGAGTACCGCATTTCGCGGGCGACGGAGGAGCTCTCACGTGGGCGGACCAAGAGCGCACGTGCCGCCTTCAGCGCTGCCCTGCTCTACCAGTACGACTGCTGGTCGGGGCTCAAGTACGCCAACGACACCCAGATGGTCAACAAAACGATGTCGTTTCTCGACGGGCTGCAAAGCAGGTCCAGCAACGCCCTCAGCATGATGTTCTCCTACGACCACTACGGCGACGACACTAGCCTGTGGGCCCCGCCGAAGACCGAGCGTGACGGGTTCTGGGAGCGGGGGTCGGGCTCGGGTCCGGGTTTCTCGGGCGGCGTGCCGAAGAATCTGAAGGCGGACGCGACGGTGTGTAAGGAGGGGTGTGAGTACCGGACGGTGCAGGAGGCGGTGGACAAGGCGCCGGAGAACTTGGAGGCAGGGAAGAGGTTCGTGATTCAGATAAAGACGGGGGTGTACGAGGAGACGGTGAGGGTGGCCTTAGGGAAGCGGAACCTGGTGTTCTTGGGCGATGGGATGGGCAAGACGGTAATTACCGGTTCGTTGAATGTGGGCCAGCCCGGAATCTCCACATACAATACGGCCACCGTCGGAGTTCTTGGTGATGGGTTCATGGCCAGTGGTCTCACAATTGCAAACACAGCAGGCCCTGATGCCCACCAGGCAGTGGCTTTCCGATCAGACAGCGATCTATCCGTGATCGAGAACTGTGAATTCTTAGGAAATCAGGACACTCTATATGCACATGGCAACCGTCAGCTATACAAATCGTGCAAAATCCAAGGCAATGTGGATTTCATCTTCGGAAACTCAGCTGCCATTTTCCAGGACTGCAACATCCTCGTTGCTCCCCGACAACTCAAACCCGAGAAGGGCGAAAACAATGCCATCACAGCCCATGGCAGGACAGACCCTGCACAGTCAACAGGTTTCGTGTTTCAAAATTGCTTGATTAACGGAACTGAAGAGTACATGAAATTGTATTACAGCAAACCCCAAGTGCACAAAAACTACTTGGGGAGGCCGTGGAAGGAGTATTCAAGGACGGTTTTCTTAAATTGCAATATGGAAGCTCTGGTTACCGCGCAGGGGTGGCTGCCTTGGAGTGGGGATTTTGCTTTGAAGACACTTTTCTATGGGGAGTACGGGAATACAGGAACAGGTTCGAATTTGGACCAGAGAGTGACCTGGAGCAGCAAGATTCCGACCGAGCATGTTAGTACATATTCAGTGCAGAATTTTATTCAGGGAGATGAGTGGATGGAGACCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

570

Amino Acids

62.43

Weight (kDa)

8.14

Isoelectric Point (pI)

30.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	69 - 214	6.1e-23	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	260 - 564	5.4e-117	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	425
Acc36I	ACCTGC	1 cut(s)	609
AccB1I	GGYRCC	1 cut(s)	838
AccB7I	CCANNNNNTGG	2 cut(s)	358, 1105
AccBSI	CCGCTC	1 cut(s)	721
AccII	CGCG	4 cut(s)	413, 460, 782, 1523
AclWI	GGATC	1 cut(s)	202
AcoI	YGGCCR	2 cut(s)	1020, 1053
AcsI	RAATTY	4 cut(s)	83, 1151, 1606, 1675
AcuI	CTGAAG	3 cut(s)	496, 791, 1422
AcvI	CACGTG	3 cut(s)	246, 482, 503
AcyI	GRCGYC	1 cut(s)	839
AfaI	GTAC	8 cut(s)	449, 536, 560, 809, 896, 1409, 1583, 1660
AfeI	AGCGCT	1 cut(s)	517
AfiI	CCNNNNNNNGG	7 cut(s)	336, 358, 1105, 1310, 1341, 1527, 1544
AflIII	ACRYGT	2 cut(s)	243, 245
AgeI	ACCGGT	1 cut(s)	974
AgsI	TTSAA	5 cut(s)	377, 985, 1379, 1479, 1561
AhdI	GACNNNNNGTC	1 cut(s)	544
AjnI	CCWGG	4 cut(s)	933, 1098, 1263, 1625
AjuI	GAANNNNNNNTTGG	2 cut(s)	74, 106
AluBI	AGCT	6 cut(s)	36, 268, 475, 1198, 1253, 1511
AluI	AGCT	6 cut(s)	36, 268, 475, 1198, 1253, 1511
Alw21I	GWGCWC	2 cut(s)	477, 1443
Alw26I	GTCTC	5 cut(s)	296, 331, 896, 1067, 1697
Alw44I	GTGCAC	1 cut(s)	1439
AlwI	GGATC	1 cut(s)	202
AlwNI	CAGNNNCTG	2 cut(s)	1088, 1352
Ama87I	CYCGRG	4 cut(s)	425, 733, 748, 1304
Aor51HI	AGCGCT	1 cut(s)	517
AoxI	GGCC	9 cut(s)	102, 348, 684, 915, 991, 1020, 1053, 1084, 1460
ApaI	GGGCCC	1 cut(s)	688
ApaLI	GTGCAC	1 cut(s)	1439
ApeKI	GCWGC	5 cut(s)	518, 610, 1253, 1534, 1632
ApoI	RAATTY	4 cut(s)	83, 1151, 1606, 1675
AsiGI	ACCGGT	1 cut(s)	974
Asp700I	GAANNNNTTC	2 cut(s)	866, 1600
AspLEI	GCGC	4 cut(s)	500, 518, 841, 1525
AspS9I	GGNCC	9 cut(s)	349, 489, 621, 684, 685, 737, 991, 1085, 1612
AsuC2I	CCSGG	3 cut(s)	323, 741, 999
AsuHPI	GGTGA	3 cut(s)	373, 919, 1052
AsuII	TTCGAA	1 cut(s)	1604
AsuNHI	GCTAGC	1 cut(s)	313
AvaI	CYCGRG	4 cut(s)	425, 733, 748, 1304
AvaII	GGWCC	4 cut(s)	489, 621, 737, 1612
AxyI	CCTNAGG	1 cut(s)	919
BaeGI	GKGCMC	2 cut(s)	688, 1443
BalI	TGGCCA	1 cut(s)	1055
BanI	GGYRCC	1 cut(s)	838
BanII	GRGCYC	5 cut(s)	342, 477, 555, 688, 734
BbrPI	CACGTG	3 cut(s)	246, 482, 503
BbsI	GAAGAC	3 cut(s)	106, 701, 1568
Bbv12I	GWGCWC	2 cut(s)	477, 1443
BbvCI	CCTCAGC	1 cut(s)	635
BbvI	GCAGC	5 cut(s)	505, 597, 1240, 1521, 1644
BccI	CCATC	9 cut(s)	314, 326, 329, 571, 944, 949, 1037, 1334, 1693
BceAI	ACGGC	4 cut(s)	182, 679, 1035, 1447
BcgI	CGANNNNNNTGC	4 cut(s)	1261, 1295, 1304, 1338
BciT130I	CCWGG	4 cut(s)	935, 1100, 1265, 1627
BcnI	CCSGG	3 cut(s)	323, 741, 999
BcoDI	GTCTC	5 cut(s)	296, 331, 896, 1067, 1697
BfaI	CTAG	2 cut(s)	314, 675
BfoI	RGCGCY	2 cut(s)	519, 842
BfuAI	ACCTGC	1 cut(s)	609
BglI	GCCNNNNNGGC	1 cut(s)	1100
Bme1390I	CCNGG	7 cut(s)	323, 741, 935, 999, 1100, 1265, 1627
Bme18I	GGWCC	4 cut(s)	489, 621, 737, 1612
BmeRI	GACNNNNNGTC	1 cut(s)	544
BmeT110I	CYCGRG	4 cut(s)	425, 733, 748, 1304
BmgT120I	GGNCC	9 cut(s)	349, 489, 621, 684, 685, 737, 991, 1085, 1612
BmiI	GGNNCC	6 cut(s)	392, 686, 687, 738, 840, 932
BmrFI	CCNGG	7 cut(s)	323, 741, 935, 999, 1100, 1265, 1627
BmsI	GCATC	1 cut(s)	1081
BmtI	GCTAGC	1 cut(s)	317
BoxI	GACNNNNGTC	1 cut(s)	246
BpiI	GAAGAC	3 cut(s)	106, 701, 1568
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
BpmI	CTGGAG	1 cut(s)	1648
Bpu10I	CCTNAGC	1 cut(s)	635
Bpu14I	TTCGAA	1 cut(s)	1604
BpuEI	CTTGAG	2 cut(s)	326, 539
BpuMI	CCSGG	3 cut(s)	323, 741, 999
BsaAI	YACGTR	3 cut(s)	246, 482, 503
BsaHI	GRCGYC	1 cut(s)	839
BsaI	GGTCTC	4 cut(s)	296, 331, 1067, 1697
BsaJI	CCNNGG	6 cut(s)	99, 384, 1219, 1336, 1463, 1538
BsaWI	WCCGGW	2 cut(s)	810, 974
BsaXI	ACNNNNNCTCC	2 cut(s)	710, 740
Bsc4I	CCNNNNNNNGG	7 cut(s)	336, 358, 1105, 1310, 1341, 1527, 1544
Bse118I	RCCGGY	1 cut(s)	974
Bse1I	ACTGG	2 cut(s)	532, 1056
Bse21I	CCTNAGG	1 cut(s)	919
Bse3DI	GCAATG	1 cut(s)	1231
BseBI	CCWGG	4 cut(s)	935, 1100, 1265, 1627
BseDI	CCNNGG	6 cut(s)	99, 384, 1219, 1336, 1463, 1538
BseGI	GGATG	4 cut(s)	318, 960, 1275, 1704
BseLI	CCNNNNNNNGG	7 cut(s)	336, 358, 1105, 1310, 1341, 1527, 1544
BseMI	GCAATG	1 cut(s)	1231
BseMII	CTCAG	3 cut(s)	218, 649, 1263
BseNI	ACTGG	2 cut(s)	532, 1056
BseRI	GAGGAG	4 cut(s)	145, 319, 485, 914
BseSI	GKGCMC	2 cut(s)	688, 1443
BseXI	GCAGC	5 cut(s)	505, 597, 1240, 1521, 1644
BseYI	CCCAGC	2 cut(s)	32, 264
BsgI	GTGCAG	3 cut(s)	839, 1338, 1691
Bsh1236I	CGCG	4 cut(s)	413, 460, 782, 1523
Bsh1285I	CGRYCG	1 cut(s)	1647
BshFI	GGCC	9 cut(s)	104, 350, 686, 917, 993, 1022, 1055, 1086, 1462
BshNI	GGYRCC	1 cut(s)	838
BshTI	ACCGGT	1 cut(s)	974
BsiEI	CGRYCG	1 cut(s)	1647
BsiHKAI	GWGCWC	2 cut(s)	477, 1443
BsiHKCI	CYCGRG	4 cut(s)	425, 733, 748, 1304
BsiSI	CCGG	6 cut(s)	322, 740, 811, 842, 975, 999
BslI	CCNNNNNNNGG	7 cut(s)	336, 358, 1105, 1310, 1341, 1527, 1544
BsmAI	GTCTC	5 cut(s)	296, 331, 896, 1067, 1697
BsmBI	CGTCTC	1 cut(s)	896
BsnI	GGCC	9 cut(s)	104, 350, 686, 917, 993, 1022, 1055, 1086, 1462
Bso31I	GGTCTC	4 cut(s)	296, 331, 1067, 1697
BsoBI	CYCGRG	4 cut(s)	425, 733, 748, 1304
Bsp119I	TTCGAA	1 cut(s)	1604
Bsp120I	GGGCCC	1 cut(s)	684
Bsp1286I	GDGCHC	6 cut(s)	342, 477, 555, 688, 734, 1443
Bsp143I	GATC	4 cut(s)	207, 1115, 1126, 1137
Bsp19I	CCATGG	2 cut(s)	99, 1336
BspANI	GGCC	9 cut(s)	104, 350, 686, 917, 993, 1022, 1055, 1086, 1462
BspCNI	CTCAG	3 cut(s)	217, 648, 1262
BspFNI	CGCG	4 cut(s)	413, 460, 782, 1523
BspLI	GGNNCC	6 cut(s)	392, 686, 687, 738, 840, 932
BspMI	ACCTGC	1 cut(s)	609
BspOI	GCTAGC	1 cut(s)	317
BspPI	GGATC	1 cut(s)	202
BspT104I	TTCGAA	1 cut(s)	1604
BspT107I	GGYRCC	1 cut(s)	838
BspTNI	GGTCTC	4 cut(s)	296, 331, 1067, 1697
BsrBI	CCGCTC	1 cut(s)	721
BsrDI	GCAATG	1 cut(s)	1231
BsrFI	RCCGGY	1 cut(s)	974
BsrI	ACTGG	2 cut(s)	532, 1056
BssAI	RCCGGY	1 cut(s)	974
BssECI	CCNNGG	6 cut(s)	99, 384, 1219, 1336, 1463, 1538
BssMI	GATC	4 cut(s)	207, 1115, 1126, 1137
BssNI	GRCGYC	1 cut(s)	839
BssT1I	CCWWGG	5 cut(s)	99, 384, 1219, 1336, 1538
Bst2UI	CCWGG	4 cut(s)	935, 1100, 1265, 1627
Bst4CI	ACNGT	9 cut(s)	787, 817, 907, 967, 1027, 1148, 1193, 1359, 1486
Bst6I	CTCTTC	4 cut(s)	95, 146, 857, 1398
BstACI	GRCGYC	1 cut(s)	839
BstBAI	YACGTR	3 cut(s)	246, 482, 503
BstBI	TTCGAA	1 cut(s)	1604
BstC8I	GCNNGC	7 cut(s)	166, 220, 315, 462, 758, 995, 1084
BstDEI	CTNAG	5 cut(s)	204, 635, 919, 1156, 1249
BstDSI	CCRYGG	3 cut(s)	99, 1336, 1463
BstEII	GGTNACC	1 cut(s)	1516
BstF5I	GGATG	4 cut(s)	318, 960, 1275, 1704
BstFNI	CGCG	4 cut(s)	413, 460, 782, 1523
BstH2I	RGCGCY	2 cut(s)	519, 842
BstHHI	GCGC	4 cut(s)	500, 518, 841, 1525
BstKTI	GATC	4 cut(s)	210, 1118, 1129, 1140
BstMAI	GTCTC	5 cut(s)	296, 331, 896, 1067, 1697
BstMBI	GATC	4 cut(s)	207, 1115, 1126, 1137
BstMCI	CGRYCG	1 cut(s)	1647
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 224, 506, 515, 624, 1079, 1100, 1331, 1531
BstNI	CCWGG	4 cut(s)	935, 1100, 1265, 1627
BstNSI	RCATGY	1 cut(s)	1655
BstPAI	GACNNNNGTC	1 cut(s)	246
BstPI	GGTNACC	1 cut(s)	1516
BstSCI	CCNGG	7 cut(s)	321, 739, 933, 997, 1098, 1263, 1625
BstSLI	GKGCMC	2 cut(s)	688, 1443
BstUI	CGCG	4 cut(s)	413, 460, 782, 1523
BstV1I	GCAGC	5 cut(s)	505, 597, 1240, 1521, 1644
BstV2I	GAAGAC	3 cut(s)	106, 701, 1568
BstX2I	RGATCY	1 cut(s)	207
BstYI	RGATCY	1 cut(s)	207
Bsu36I	CCTNAGG	1 cut(s)	919
BsuRI	GGCC	9 cut(s)	104, 350, 686, 917, 993, 1022, 1055, 1086, 1462
BtgI	CCRYGG	3 cut(s)	99, 1336, 1463
BtgZI	GCGATG	1 cut(s)	963
BtsCI	GGATG	4 cut(s)	318, 960, 1275, 1704
BtsI	GCAGTG	1 cut(s)	1110
BtsIMutI	CAGTG	3 cut(s)	1063, 1110, 1674
BveI	ACCTGC	1 cut(s)	609
Cac8I	GCNNGC	7 cut(s)	166, 220, 315, 462, 758, 995, 1084
CaiI	CAGNNNCTG	2 cut(s)	1088, 1352
CfoI	GCGC	4 cut(s)	500, 518, 841, 1525
Cfr10I	RCCGGY	1 cut(s)	974
Cfr13I	GGNCC	9 cut(s)	349, 489, 621, 684, 685, 737, 991, 1085, 1612
CseI	GACGC	2 cut(s)	369, 788
CsiI	ACCWGGT	1 cut(s)	933
Csp6I	GTAC	8 cut(s)	448, 535, 559, 808, 895, 1408, 1582, 1659
CspAI	ACCGGT	1 cut(s)	974
CviAII	CATG	8 cut(s)	57, 100, 640, 1051, 1184, 1337, 1411, 1652
CviQI	GTAC	8 cut(s)	448, 535, 559, 808, 895, 1408, 1582, 1659
DdeI	CTNAG	5 cut(s)	204, 635, 919, 1156, 1249
DinI	GGCGCC	1 cut(s)	840
DpnI	GATC	4 cut(s)	209, 1117, 1128, 1139
DpnII	GATC	4 cut(s)	207, 1115, 1126, 1137
DriI	GACNNNNNGTC	1 cut(s)	544
EaeI	YGGCCR	2 cut(s)	1020, 1053
Eam1104I	CTCTTC	4 cut(s)	95, 146, 857, 1398
Eam1105I	GACNNNNNGTC	1 cut(s)	544
EarI	CTCTTC	4 cut(s)	95, 146, 857, 1398
EciI	GGCGGA	4 cut(s)	148, 172, 502, 791
Ecl136II	GAGCTC	1 cut(s)	475
Eco130I	CCWWGG	5 cut(s)	99, 384, 1219, 1336, 1538
Eco24I	GRGCYC	5 cut(s)	342, 477, 555, 688, 734
Eco31I	GGTCTC	4 cut(s)	296, 331, 1067, 1697
Eco47I	GGWCC	4 cut(s)	489, 621, 737, 1612
Eco47III	AGCGCT	1 cut(s)	517
Eco53kI	GAGCTC	1 cut(s)	475
Eco57I	CTGAAG	3 cut(s)	496, 791, 1422
Eco72I	CACGTG	3 cut(s)	246, 482, 503
Eco81I	CCTNAGG	1 cut(s)	919
Eco88I	CYCGRG	4 cut(s)	425, 733, 748, 1304
Eco91I	GGTNACC	1 cut(s)	1516
EcoICRI	GAGCTC	1 cut(s)	475
EcoO109I	RGGNCCY	2 cut(s)	685, 1085
EcoO65I	GGTNACC	1 cut(s)	1516
EcoRI	GAATTC	1 cut(s)	1151
EcoRII	CCWGG	4 cut(s)	933, 1098, 1263, 1625
EcoT14I	CCWWGG	5 cut(s)	99, 384, 1219, 1336, 1538
EcoT38I	GRGCYC	5 cut(s)	342, 477, 555, 688, 734
EgeI	GGCGCC	1 cut(s)	840
EheI	GGCGCC	1 cut(s)	840
ErhI	CCWWGG	5 cut(s)	99, 384, 1219, 1336, 1538
Esp3I	CGTCTC	1 cut(s)	896
FaeI	CATG	8 cut(s)	60, 103, 643, 1054, 1187, 1340, 1414, 1655
FatI	CATG	8 cut(s)	56, 99, 639, 1050, 1183, 1336, 1410, 1651
FauI	CCCGC	5 cut(s)	173, 239, 453, 696, 714
FokI	GGATG	3 cut(s)	305, 967, 1262
FriOI	GRGCYC	5 cut(s)	342, 477, 555, 688, 734
FspBI	CTAG	2 cut(s)	314, 675
GlaI	GCGC	4 cut(s)	499, 517, 840, 1524
GsaI	CCCAGC	2 cut(s)	36, 268
GsuI	CTGGAG	1 cut(s)	1648
HaeII	RGCGCY	2 cut(s)	519, 842
HaeIII	GGCC	9 cut(s)	104, 350, 686, 917, 993, 1022, 1055, 1086, 1462
HapII	CCGG	6 cut(s)	322, 740, 811, 842, 975, 999
HgaI	GACGC	2 cut(s)	369, 788
HhaI	GCGC	4 cut(s)	500, 518, 841, 1525
Hin1I	GRCGYC	1 cut(s)	839
Hin1II	CATG	8 cut(s)	60, 103, 643, 1054, 1187, 1340, 1414, 1655
Hin6I	GCGC	4 cut(s)	498, 516, 839, 1523
HinP1I	GCGC	4 cut(s)	498, 516, 839, 1523
HincII	GTYRAC	2 cut(s)	583, 1362
HindII	GTYRAC	2 cut(s)	583, 1362
HinfI	GANTC	4 cut(s)	766, 874, 1002, 1639
HpaII	CCGG	6 cut(s)	322, 740, 811, 842, 975, 999
HphI	GGTGA	3 cut(s)	373, 919, 1052
Hpy166II	GTNNAC	5 cut(s)	583, 832, 895, 1362, 1441
Hpy188III	TCNNGA	7 cut(s)	301, 374, 434, 602, 871, 1141, 1166
Hpy8I	GTNNAC	5 cut(s)	583, 832, 895, 1362, 1441
Hpy99I	CGWCG	5 cut(s)	469, 608, 671, 787, 1031
HpyAV	CCTTC	7 cut(s)	118, 189, 287, 520, 766, 1303, 1462
HpyCH4III	ACNGT	9 cut(s)	787, 817, 907, 967, 1027, 1148, 1193, 1359, 1486
HpyCH4IV	ACGT	3 cut(s)	245, 481, 502
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 224, 506, 515, 624, 1079, 1100, 1331, 1531
HpyF3I	CTNAG	5 cut(s)	204, 635, 919, 1156, 1249
HpySE526I	ACGT	3 cut(s)	245, 481, 502
Hsp92I	GRCGYC	1 cut(s)	839
Hsp92II	CATG	8 cut(s)	60, 103, 643, 1054, 1187, 1340, 1414, 1655
HspAI	GCGC	4 cut(s)	498, 516, 839, 1523
KasI	GGCGCC	1 cut(s)	838
Kzo9I	GATC	4 cut(s)	207, 1115, 1126, 1137
LmnI	GCTCC	5 cut(s)	273, 472, 718, 1291, 1629
Lsp1109I	GCAGC	5 cut(s)	505, 597, 1240, 1521, 1644
LweI	GCATC	1 cut(s)	1081
MabI	ACCWGGT	1 cut(s)	933
MaeI	CTAG	2 cut(s)	314, 675
MaeII	ACGT	3 cut(s)	245, 481, 502
MaeIII	GTNAC	5 cut(s)	199, 361, 704, 1516, 1621
MalI	GATC	4 cut(s)	209, 1117, 1128, 1139
MbiI	CCGCTC	1 cut(s)	721
MboI	GATC	4 cut(s)	207, 1115, 1126, 1137
MboII	GAAGA	9 cut(s)	82, 106, 133, 706, 775, 874, 1231, 1415, 1573
MfeI	CAATTG	1 cut(s)	1068
MflI	RGATCY	1 cut(s)	207
MhlI	GDGCHC	6 cut(s)	342, 477, 555, 688, 734, 1443
MlsI	TGGCCA	1 cut(s)	1055
MluCI	AATT	9 cut(s)	83, 969, 1068, 1151, 1381, 1415, 1495, 1606, 1675
MluNI	TGGCCA	1 cut(s)	1055
Mly113I	GGCGCC	1 cut(s)	839
MmeI	TCCRAC	3 cut(s)	235, 1009, 1667
Mox20I	TGGCCA	1 cut(s)	1055
MroXI	GAANNNNTTC	2 cut(s)	866, 1600
MscI	TGGCCA	1 cut(s)	1055
MseI	TTAA	2 cut(s)	1392, 1493
MslI	CAYNNNNRTG	2 cut(s)	98, 575
Msp20I	TGGCCA	1 cut(s)	1055
MspA1I	CMGCKG	1 cut(s)	1253
MspI	CCGG	6 cut(s)	322, 740, 811, 842, 975, 999
MspR9I	CCNGG	7 cut(s)	323, 741, 935, 999, 1100, 1265, 1627
MunI	CAATTG	1 cut(s)	1068
MvaI	CCWGG	4 cut(s)	935, 1100, 1265, 1627
MvnI	CGCG	4 cut(s)	413, 460, 782, 1523
MwoI	GCNNNNNNNGC	9 cut(s)	191, 224, 506, 515, 624, 1079, 1100, 1331, 1531
NarI	GGCGCC	1 cut(s)	839
NciI	CCSGG	3 cut(s)	323, 741, 999
NcoI	CCATGG	2 cut(s)	99, 1336
NdeII	GATC	4 cut(s)	207, 1115, 1126, 1137
NheI	GCTAGC	1 cut(s)	313
NlaIII	CATG	8 cut(s)	60, 103, 643, 1054, 1187, 1340, 1414, 1655
NlaIV	GGNNCC	6 cut(s)	392, 686, 687, 738, 840, 932
NmuCI	GTSAC	4 cut(s)	199, 361, 704, 1621
NspI	RCATGY	1 cut(s)	1655
NspV	TTCGAA	1 cut(s)	1604
PaeR7I	CTCGAG	1 cut(s)	425
PdmI	GAANNNNTTC	2 cut(s)	866, 1600
PfeI	GAWTC	4 cut(s)	766, 874, 1002, 1639
PflMI	CCANNNNNTGG	2 cut(s)	358, 1105
PfoI	TCCNGGA	1 cut(s)	1263
PinAI	ACCGGT	1 cut(s)	974
PluTI	GGCGCC	1 cut(s)	842
PmaCI	CACGTG	3 cut(s)	246, 482, 503
PmlI	CACGTG	3 cut(s)	246, 482, 503
Ppu21I	YACGTR	3 cut(s)	246, 482, 503
PshAI	GACNNNNGTC	1 cut(s)	246
Psp124BI	GAGCTC	1 cut(s)	477
Psp6I	CCWGG	4 cut(s)	933, 1098, 1263, 1625
PspCI	CACGTG	3 cut(s)	246, 482, 503
PspEI	GGTNACC	1 cut(s)	1516
PspFI	CCCAGC	2 cut(s)	32, 264
PspGI	CCWGG	4 cut(s)	933, 1098, 1263, 1625
PspN4I	GGNNCC	6 cut(s)	392, 686, 687, 738, 840, 932
PspOMI	GGGCCC	1 cut(s)	684
PspPI	GGNCC	9 cut(s)	349, 489, 621, 684, 685, 737, 991, 1085, 1612
PspXI	VCTCGAGB	1 cut(s)	425
PstNI	CAGNNNCTG	2 cut(s)	1088, 1352
PsuI	RGATCY	1 cut(s)	207
PvuII	CAGCTG	1 cut(s)	1253
RsaI	GTAC	8 cut(s)	449, 536, 560, 809, 896, 1409, 1583, 1660
RsaNI	GTAC	8 cut(s)	448, 535, 559, 808, 895, 1408, 1582, 1659
RseI	CAYNNNNRTG	2 cut(s)	98, 575
SacI	GAGCTC	1 cut(s)	477
SaqAI	TTAA	2 cut(s)	1392, 1493
Sau3AI	GATC	4 cut(s)	207, 1115, 1126, 1137
Sau96I	GGNCC	9 cut(s)	349, 489, 621, 684, 685, 737, 991, 1085, 1612
ScrFI	CCNGG	7 cut(s)	323, 741, 935, 999, 1100, 1265, 1627
SduI	GDGCHC	6 cut(s)	342, 477, 555, 688, 734, 1443
SexAI	ACCWGGT	1 cut(s)	933
SfaNI	GCATC	1 cut(s)	1081
SfoI	GGCGCC	1 cut(s)	840
Sfr274I	CTCGAG	1 cut(s)	425
SfuI	TTCGAA	1 cut(s)	1604
SinI	GGWCC	4 cut(s)	489, 621, 737, 1612
SlaI	CTCGAG	1 cut(s)	425
SmiMI	CAYNNNNRTG	2 cut(s)	98, 575
SmlI	CTYRAG	3 cut(s)	341, 425, 554
SmoI	CTYRAG	3 cut(s)	341, 425, 554
Sse9I	AATT	9 cut(s)	83, 969, 1068, 1151, 1381, 1415, 1495, 1606, 1675
SspDI	GGCGCC	1 cut(s)	838
SspMI	CTAG	2 cut(s)	314, 675
SstI	GAGCTC	1 cut(s)	477
StyD4I	CCNGG	7 cut(s)	321, 739, 933, 997, 1098, 1263, 1625
StyI	CCWWGG	5 cut(s)	99, 384, 1219, 1336, 1538
TaaI	ACNGT	9 cut(s)	787, 817, 907, 967, 1027, 1148, 1193, 1359, 1486
TaiI	ACGT	3 cut(s)	248, 484, 505
TaqI	TCGA	5 cut(s)	300, 426, 603, 1140, 1604
TaqII	GACCGA	2 cut(s)	713, 1661
TasI	AATT	9 cut(s)	83, 969, 1068, 1151, 1381, 1415, 1495, 1606, 1675
TatI	WGTACW	2 cut(s)	1407, 1658
TauI	GCSGC	6 cut(s)	164, 194, 197, 413, 509, 756
TfiI	GAWTC	4 cut(s)	766, 874, 1002, 1639
Tru1I	TTAA	2 cut(s)	1392, 1493
Tru9I	TTAA	2 cut(s)	1392, 1493
TscAI	CASTG	3 cut(s)	1063, 1110, 1674
TseFI	GTSAC	4 cut(s)	199, 361, 704, 1621
TseI	GCWGC	5 cut(s)	518, 610, 1253, 1534, 1632
Tsp45I	GTSAC	4 cut(s)	199, 361, 704, 1621
TspDTI	ATGAA	4 cut(s)	82, 1039, 1225, 1427
TspGWI	ACGGA	4 cut(s)	380, 482, 1123, 1410
TspRI	CASTG	3 cut(s)	1063, 1110, 1674
Van91I	CCANNNNNTGG	2 cut(s)	358, 1105
VneI	GTGCAC	1 cut(s)	1439
VpaK11BI	GGWCC	4 cut(s)	489, 621, 737, 1612
XapI	RAATTY	4 cut(s)	83, 1151, 1606, 1675
XceI	RCATGY	1 cut(s)	1655
XhoI	CTCGAG	1 cut(s)	425
XmnI	GAANNNNTTC	2 cut(s)	866, 1600
XspI	CTAG	2 cut(s)	314, 675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.