Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Reverse (-)

28666054 .. 28669479

Gene Structure 3426 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1238900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGACCTTCAATTTCCACAGAAAACCCACCAAATTTATAAAGCCACCACCATGCTGCTCCTCCATACCCAAATCCCTAATCCTCATTCTCCTCATGGCCTCCCTCCTCTTCCTTTCTCTCCTCTCCCTCACTCTCTTCCTCTCTCTATCCTCCGCTTCCCGCCACCGTCCTCCCGGTCACTCCATCTCCCCTCAACCCCAGATCCAGCTAGCCTGCAAGGCCACGCGCTTCCCAGAAGCGTGCCAAGCTTCCCTGGGCAAGGTCGTCACTGACCCAAATGCCACCCCTCTCGAGACCATCCATTTCGCCGTCCAGGTCGCTGACGACGGCCTCAAAACGGCGCAGGGCATGGTCCACACCATCCTGGACTCGTCCGCAGGGAACATAAACCGATCCACCGCCGCGAAAAACTGCCTCGACGTCCTTGGCAACTCCCACTACCGAATTTCGCTCGCCACCGATGGATTGTCACGTGGTCGCGTTAAAAACGCACGTGCCTCCATGAGCGCCGCGTTGCTCTACCAGTACGACTGCTGGTCGGCTCTCAAGTACGCAAACGACACCCACATGGTAAACGAGACGATGTCGTTTCTCGACTCGCTGATCCGCAAATCCAGCAACGCGCTCAGCATGATCGCGTCGTACGACAACTTCGGAAACGACACAAAGTCGTGGGGCCCGCCGAAAACGGAGCGGGACGGGTTCTGGGAGCGGGTCTCGGGCGGAGGGTCTGACCAGGGATTTCGGGGAGGGATTCCGTCAGGTTTAAAGGCGGACGTGACGGTGTGCAAGGAGAATTCGTGCGATTACAAGACGGTGCAGGAGGCTGTGAATGCCGCGCCGGATAACGCGGGAGGTAAGAGGTTCGTGATTGGGATAAAGGCGGGGGTGTACGAGGAGACCGTAGGGGTGCCCTTAGAGAAGCGGAACGTGGTGTTCTTGGGTGACGGGATAGGCAAAACCGTCATTACGGGCTCATTAAATGTAGGCCAGCCCGGAATTTCCACCTACAACACTGCCACCGTCGGAGTTAACGGTGATGGGTTCATGGCGAGTGGTCTCACAATCCAGAACACCGCAGGTCCCGATGCTCACCAAGCGGTTGCTTTCAGATCAGACAGTGATCTATCCGTAATCGAGAACTGCGAATTCATTGGCAATCAGGACACTCTCTATGCTCACGGCAACCGCCAATTCTACAAGTCGTGCACCATCCACGGTAATGTTGATTTCATCTTTGGTAACTCAGCTTCCATCTTCCAAGACTGCACCATCCTTGTCCGTCCTAGACAACTCAAACCCGAGAAAGGCGAAAACAATGCAGTCACTGCCCAAGGAAGAACAGACCCTGCGCAGTCAACGGGTTTTGTATTTCAAAACTGCTTGATCAACGGCACGGAAGAGTACATGAAGTTATATTGGAGCAAGCCCAAAGTGCACAAAAACTTCTTGGGGAGGCCGTGGAAGGAGTACTCGAGGACGGTGTTTATAAACTGTAATATGGAAGCTCTTCTAACACCACAAGGGTGGATGCCTTGGAGTGGAGATTTTGCTTTGAAAACCCTTTTCTATGGCGAATTCGGGAATTCGGGTGCGGGTTCTGATTTGTCCCAGAGAGTGAACTGGAGCAGCAAGATCCCACCCGAGCATGTTAATACGTATTCTCTGCAGAATTTTATCCAGGGAGATGAGTGGATTAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

569

Amino Acids

61.94

Weight (kDa)

8.44

Isoelectric Point (pI)

33.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	67 - 211	8.8e-20	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	259 - 563	2.5e-120	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	38, 1490
AatII	GACGTC	1 cut(s)	423
Acc16I	TGCGCA	1 cut(s)	1353
Acc36I	ACCTGC	1 cut(s)	1070
AccB1I	GGYRCC	1 cut(s)	910
AccBSI	CCGCTC	2 cut(s)	694, 712
AccII	CGCG	8 cut(s)	226, 404, 480, 512, 623, 638, 839, 851
AclWI	GGATC	4 cut(s)	196, 387, 598, 1630
AcsI	RAATTY	8 cut(s)	32, 444, 796, 999, 1148, 1577, 1585, 1672
AcvI	CACGTG	2 cut(s)	473, 494
AcyI	GRCGYC	1 cut(s)	420
AfaI	GTAC	6 cut(s)	527, 551, 644, 893, 1406, 1472
AfiI	CCNNNNNNNGG	4 cut(s)	259, 337, 1307, 1541
AgsI	TTSAA	3 cut(s)	10, 1376, 1558
AhdI	GACNNNNNGTC	2 cut(s)	535, 667
AjiI	CACGTC	1 cut(s)	778
AjnI	CCWGG	5 cut(s)	252, 312, 363, 735, 1680
AleI	CACNNNNGTG	1 cut(s)	1525
AluBI	AGCT	4 cut(s)	208, 248, 1250, 1508
AluI	AGCT	4 cut(s)	208, 248, 1250, 1508
Alw21I	GWGCWC	2 cut(s)	1211, 1440
Alw26I	GTCTC	5 cut(s)	287, 572, 721, 893, 1064
Alw44I	GTGCAC	2 cut(s)	1207, 1436
AlwI	GGATC	4 cut(s)	196, 387, 598, 1630
AlwNI	CAGNNNCTG	2 cut(s)	1328, 1349
Ama87I	CYCGRG	5 cut(s)	290, 718, 1301, 1474, 1643
AoxI	GGCC	6 cut(s)	96, 219, 328, 676, 988, 1457
ApaI	GGGCCC	1 cut(s)	680
ApaLI	GTGCAC	2 cut(s)	1207, 1436
ApeKI	GCWGC	2 cut(s)	54, 1629
ApoI	RAATTY	8 cut(s)	32, 444, 796, 999, 1148, 1577, 1585, 1672
Asp700I	GAANNNNTTC	1 cut(s)	1509
AspLEI	GCGC	6 cut(s)	228, 343, 509, 625, 841, 1354
AspS9I	GGNCC	4 cut(s)	352, 676, 677, 1082
AsuC2I	CCSGG	2 cut(s)	174, 996
AsuHPI	GGTGA	3 cut(s)	956, 1049, 1085
AsuNHI	GCTAGC	1 cut(s)	208
AvaI	CYCGRG	5 cut(s)	290, 718, 1301, 1474, 1643
AvaII	GGWCC	2 cut(s)	352, 1082
BaeGI	GKGCMC	4 cut(s)	680, 915, 1211, 1440
BanI	GGYRCC	1 cut(s)	910
BanII	GRGCYC	2 cut(s)	680, 977
BarI	GAAGNNNNNNTAC	4 cut(s)	635, 667, 1234, 1266
BbrPI	CACGTG	2 cut(s)	473, 494
Bbv12I	GWGCWC	2 cut(s)	1211, 1440
BbvI	GCAGC	2 cut(s)	41, 1641
BccI	CCATC	8 cut(s)	191, 305, 368, 455, 1034, 1220, 1262, 1280
BceAI	ACGGC	6 cut(s)	293, 343, 354, 1198, 1408, 1444
BcgI	CGANNNNNNTGC	2 cut(s)	1301, 1335
BciT130I	CCWGG	5 cut(s)	254, 314, 365, 737, 1682
BclI	TGATCA	1 cut(s)	1386
BcnI	CCSGG	2 cut(s)	174, 996
BcoDI	GTCTC	5 cut(s)	287, 572, 721, 893, 1064
BfaI	CTAG	2 cut(s)	209, 1287
BfmI	CTRYAG	1 cut(s)	1667
BfoI	RGCGCY	1 cut(s)	510
BfuAI	ACCTGC	1 cut(s)	1070
BglI	GCCNNNNNGGC	1 cut(s)	218
BisI	GCNGC	5 cut(s)	55, 402, 510, 837, 1630
BlpI	GCTNAGC	1 cut(s)	626
BlsI	GCNGC	5 cut(s)	56, 403, 511, 838, 1631
BmcAI	AGTACT	1 cut(s)	1472
Bme1390I	CCNGG	7 cut(s)	174, 254, 314, 365, 737, 996, 1682
Bme18I	GGWCC	2 cut(s)	352, 1082
BmeRI	GACNNNNNGTC	2 cut(s)	535, 667
BmeT110I	CYCGRG	5 cut(s)	290, 718, 1301, 1474, 1643
BmgBI	CACGTC	1 cut(s)	778
BmgT120I	GGNCC	4 cut(s)	352, 676, 677, 1082
BmiI	GGNNCC	4 cut(s)	677, 678, 912, 1084
BmrFI	CCNGG	7 cut(s)	174, 254, 314, 365, 737, 996, 1682
BmsI	GCATC	2 cut(s)	1078, 1521
BmtI	GCTAGC	1 cut(s)	212
BplI	GAGNNNNNCTC	2 cut(s)	701, 733
BpmI	CTGGAG	1 cut(s)	1645
Bpu1102I	GCTNAGC	1 cut(s)	626
BpuEI	CTTGAG	1 cut(s)	530
BpuMI	CCSGG	2 cut(s)	174, 996
BsaAI	YACGTR	3 cut(s)	473, 494, 1659
BsaHI	GRCGYC	1 cut(s)	420
BsaI	GGTCTC	4 cut(s)	287, 721, 893, 1064
BsaJI	CCNNGG	9 cut(s)	252, 253, 424, 736, 1216, 1333, 1460, 1535, 1681
BsaXI	ACNNNNNCTCC	4 cut(s)	170, 200, 785, 815
Bsc4I	CCNNNNNNNGG	4 cut(s)	259, 337, 1307, 1541
Bse1I	ACTGG	2 cut(s)	523, 1628
BseBI	CCWGG	5 cut(s)	254, 314, 365, 737, 1682
BseDI	CCNNGG	9 cut(s)	252, 253, 424, 736, 1216, 1333, 1460, 1535, 1681
BseGI	GGATG	5 cut(s)	297, 360, 1212, 1272, 1536
BseLI	CCNNNNNNNGG	4 cut(s)	259, 337, 1307, 1541
BseMII	CTCAG	2 cut(s)	640, 1260
BseNI	ACTGG	2 cut(s)	523, 1628
BseRI	GAGGAG	5 cut(s)	49, 80, 95, 110, 911
BseSI	GKGCMC	4 cut(s)	680, 915, 1211, 1440
BseXI	GCAGC	2 cut(s)	41, 1641
BsgI	GTGCAG	2 cut(s)	839, 1252
Bsh1236I	CGCG	8 cut(s)	226, 404, 480, 512, 623, 638, 839, 851
BshFI	GGCC	6 cut(s)	98, 221, 330, 678, 990, 1459
BshNI	GGYRCC	1 cut(s)	910
BsiHKAI	GWGCWC	2 cut(s)	1211, 1440
BsiHKCI	CYCGRG	5 cut(s)	290, 718, 1301, 1474, 1643
BsiSI	CCGG	3 cut(s)	174, 842, 996
BsiWI	CGTACG	1 cut(s)	642
BslFI	GGGAC	3 cut(s)	710, 1068, 1594
BslI	CCNNNNNNNGG	4 cut(s)	259, 337, 1307, 1541
BsmAI	GTCTC	5 cut(s)	287, 572, 721, 893, 1064
BsmBI	CGTCTC	1 cut(s)	572
BsmFI	GGGAC	3 cut(s)	710, 1068, 1594
BsmI	GAATGC	1 cut(s)	838
BsnI	GGCC	6 cut(s)	98, 221, 330, 678, 990, 1459
Bso31I	GGTCTC	4 cut(s)	287, 721, 893, 1064
BsoBI	CYCGRG	5 cut(s)	290, 718, 1301, 1474, 1643
Bsp120I	GGGCCC	1 cut(s)	676
Bsp1286I	GDGCHC	5 cut(s)	680, 915, 977, 1211, 1440
Bsp143I	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
Bsp1720I	GCTNAGC	1 cut(s)	626
BspANI	GGCC	6 cut(s)	98, 221, 330, 678, 990, 1459
BspCNI	CTCAG	2 cut(s)	639, 1259
BspFNI	CGCG	8 cut(s)	226, 404, 480, 512, 623, 638, 839, 851
BspLI	GGNNCC	4 cut(s)	677, 678, 912, 1084
BspMAI	CTGCAG	1 cut(s)	1671
BspMI	ACCTGC	1 cut(s)	1070
BspOI	GCTAGC	1 cut(s)	212
BspPI	GGATC	4 cut(s)	196, 387, 598, 1630
BspQI	GCTCTTC	1 cut(s)	1515
BspT107I	GGYRCC	1 cut(s)	910
BspTNI	GGTCTC	4 cut(s)	287, 721, 893, 1064
BsrBI	CCGCTC	2 cut(s)	694, 712
BsrI	ACTGG	2 cut(s)	523, 1628
BssECI	CCNNGG	9 cut(s)	252, 253, 424, 736, 1216, 1333, 1460, 1535, 1681
BssMI	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
BssNI	GRCGYC	1 cut(s)	420
BssT1I	CCWWGG	3 cut(s)	424, 1333, 1535
Bst2UI	CCWGG	5 cut(s)	254, 314, 365, 737, 1682
Bst6I	CTCTTC	4 cut(s)	113, 140, 1395, 1515
BstACI	GRCGYC	1 cut(s)	420
BstAPI	GCANNNNNTGC	1 cut(s)	1328
BstBAI	YACGTR	3 cut(s)	473, 494, 1659
BstC8I	GCNNGC	7 cut(s)	210, 214, 241, 453, 680, 992, 1427
BstDEI	CTNAG	3 cut(s)	626, 916, 1246
BstDSI	CCRYGG	2 cut(s)	1216, 1460
BstENI	CCTNNNNNAGG	1 cut(s)	257
BstF5I	GGATG	5 cut(s)	297, 360, 1212, 1272, 1536
BstFNI	CGCG	8 cut(s)	226, 404, 480, 512, 623, 638, 839, 851
BstH2I	RGCGCY	1 cut(s)	510
BstHHI	GCGC	6 cut(s)	228, 343, 509, 625, 841, 1354
BstKTI	GATC	8 cut(s)	204, 395, 606, 636, 1115, 1126, 1389, 1638
BstMAI	GTCTC	5 cut(s)	287, 572, 721, 893, 1064
BstMBI	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
BstMWI	GCNNNNNNNGC	7 cut(s)	218, 245, 615, 833, 1097, 1328, 1435
BstNI	CCWGG	5 cut(s)	254, 314, 365, 737, 1682
BstNSI	RCATGY	1 cut(s)	1652
BstSCI	CCNGG	7 cut(s)	172, 252, 312, 363, 735, 994, 1680
BstSFI	CTRYAG	1 cut(s)	1667
BstSLI	GKGCMC	4 cut(s)	680, 915, 1211, 1440
BstSNI	TACGTA	1 cut(s)	1659
BstUI	CGCG	8 cut(s)	226, 404, 480, 512, 623, 638, 839, 851
BstV1I	GCAGC	2 cut(s)	41, 1641
BstX2I	RGATCY	2 cut(s)	201, 1635
BstXI	CCANNNNNNTGG	1 cut(s)	1527
BstYI	RGATCY	2 cut(s)	201, 1635
BsuRI	GGCC	6 cut(s)	98, 221, 330, 678, 990, 1459
BtgI	CCRYGG	2 cut(s)	1216, 1460
BtrI	CACGTC	1 cut(s)	778
BtsCI	GGATG	5 cut(s)	297, 360, 1212, 1272, 1536
BtsI	GCAGTG	2 cut(s)	1014, 1326
BtsIMutI	CAGTG	4 cut(s)	267, 1014, 1126, 1326
BveI	ACCTGC	1 cut(s)	1070
Cac8I	GCNNGC	7 cut(s)	210, 214, 241, 453, 680, 992, 1427
CaiI	CAGNNNCTG	2 cut(s)	1328, 1349
CfoI	GCGC	6 cut(s)	228, 343, 509, 625, 841, 1354
Cfr13I	GGNCC	4 cut(s)	352, 676, 677, 1082
CseI	GACGC	1 cut(s)	627
Csp6I	GTAC	6 cut(s)	526, 550, 643, 892, 1405, 1471
CviQI	GTAC	6 cut(s)	526, 550, 643, 892, 1405, 1471
DdeI	CTNAG	3 cut(s)	626, 916, 1246
DpnI	GATC	8 cut(s)	203, 394, 605, 635, 1114, 1125, 1388, 1637
DpnII	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
DraI	TTTAAA	1 cut(s)	768
DriI	GACNNNNNGTC	2 cut(s)	535, 667
Eam1104I	CTCTTC	4 cut(s)	113, 140, 1395, 1515
Eam1105I	GACNNNNNGTC	2 cut(s)	535, 667
EarI	CTCTTC	4 cut(s)	113, 140, 1395, 1515
EciI	GGCGGA	2 cut(s)	738, 788
Eco105I	TACGTA	1 cut(s)	1659
Eco130I	CCWWGG	3 cut(s)	424, 1333, 1535
Eco24I	GRGCYC	2 cut(s)	680, 977
Eco31I	GGTCTC	4 cut(s)	287, 721, 893, 1064
Eco47I	GGWCC	2 cut(s)	352, 1082
Eco72I	CACGTG	2 cut(s)	473, 494
Eco88I	CYCGRG	5 cut(s)	290, 718, 1301, 1474, 1643
EcoNI	CCTNNNNNAGG	1 cut(s)	257
EcoO109I	RGGNCCY	2 cut(s)	676, 1082
EcoRI	GAATTC	4 cut(s)	796, 1148, 1577, 1585
EcoRII	CCWGG	5 cut(s)	252, 312, 363, 735, 1680
EcoT14I	CCWWGG	3 cut(s)	424, 1333, 1535
EcoT38I	GRGCYC	2 cut(s)	680, 977
ErhI	CCWWGG	3 cut(s)	424, 1333, 1535
Esp3I	CGTCTC	1 cut(s)	572
FaqI	GGGAC	3 cut(s)	710, 1068, 1594
FauI	CCCGC	7 cut(s)	167, 687, 687, 705, 844, 877, 1588
FbaI	TGATCA	1 cut(s)	1386
Fnu4HI	GCNGC	5 cut(s)	55, 402, 510, 837, 1630
FokI	GGATG	5 cut(s)	284, 347, 1199, 1259, 1543
FriOI	GRGCYC	2 cut(s)	680, 977
Fsp4HI	GCNGC	5 cut(s)	55, 402, 510, 837, 1630
FspBI	CTAG	2 cut(s)	209, 1287
FspI	TGCGCA	1 cut(s)	1353
GlaI	GCGC	6 cut(s)	227, 342, 508, 624, 840, 1353
GluI	GCNGC	5 cut(s)	55, 402, 510, 837, 1630
GsuI	CTGGAG	1 cut(s)	1645
HaeII	RGCGCY	1 cut(s)	510
HaeIII	GGCC	6 cut(s)	98, 221, 330, 678, 990, 1459
HapII	CCGG	3 cut(s)	174, 842, 996
HgaI	GACGC	1 cut(s)	627
HhaI	GCGC	6 cut(s)	228, 343, 509, 625, 841, 1354
Hin1I	GRCGYC	1 cut(s)	420
Hin6I	GCGC	6 cut(s)	226, 341, 507, 623, 839, 1352
HinP1I	GCGC	6 cut(s)	226, 341, 507, 623, 839, 1352
HincII	GTYRAC	2 cut(s)	1033, 1359
HindII	GTYRAC	2 cut(s)	1033, 1359
HindIII	AAGCTT	1 cut(s)	246
HinfI	GANTC	3 cut(s)	368, 596, 754
HpaI	GTTAAC	1 cut(s)	1033
HpaII	CCGG	3 cut(s)	174, 842, 996
HphI	GGTGA	3 cut(s)	956, 1049, 1085
Hpy166II	GTNNAC	8 cut(s)	355, 574, 892, 1033, 1209, 1359, 1438, 1621
Hpy188I	TCNGA	6 cut(s)	656, 733, 1028, 1112, 1117, 1603
Hpy188III	TCNNGA	9 cut(s)	290, 292, 593, 868, 1069, 1085, 1138, 1163, 1582
Hpy8I	GTNNAC	8 cut(s)	355, 574, 892, 1033, 1209, 1359, 1438, 1621
Hpy99I	CGWCG	4 cut(s)	329, 422, 643, 1028
HpyAV	CCTTC	2 cut(s)	16, 1459
HpyCH4IV	ACGT	6 cut(s)	420, 472, 493, 777, 930, 1658
HpyCH4V	TGCA	8 cut(s)	216, 789, 820, 1209, 1269, 1322, 1438, 1669
HpyF10VI	GCNNNNNNNGC	7 cut(s)	218, 245, 615, 833, 1097, 1328, 1435
HpyF3I	CTNAG	3 cut(s)	626, 916, 1246
HpySE526I	ACGT	6 cut(s)	420, 472, 493, 777, 930, 1658
Hsp92I	GRCGYC	1 cut(s)	420
HspAI	GCGC	6 cut(s)	226, 341, 507, 623, 839, 1352
Ksp22I	TGATCA	1 cut(s)	1386
KspAI	GTTAAC	1 cut(s)	1033
Kzo9I	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
LguI	GCTCTTC	1 cut(s)	1515
LmnI	GCTCC	5 cut(s)	62, 691, 709, 1422, 1626
Lsp1109I	GCAGC	2 cut(s)	41, 1641
LweI	GCATC	2 cut(s)	1078, 1521
MaeI	CTAG	2 cut(s)	209, 1287
MaeII	ACGT	6 cut(s)	420, 472, 493, 777, 930, 1658
MaeIII	GTNAC	7 cut(s)	176, 265, 468, 778, 944, 1241, 1324
MalI	GATC	8 cut(s)	203, 394, 605, 635, 1114, 1125, 1388, 1637
MbiI	CCGCTC	2 cut(s)	694, 712
MboI	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
MboII	GAAGA	6 cut(s)	100, 127, 1249, 1350, 1412, 1502
MflI	RGATCY	2 cut(s)	201, 1635
MhlI	GDGCHC	5 cut(s)	680, 915, 977, 1211, 1440
MlyI	GAGTC	2 cut(s)	362, 590
MmeI	TCCRAC	1 cut(s)	1006
MroXI	GAANNNNTTC	1 cut(s)	1509
MseI	TTAA	6 cut(s)	483, 767, 980, 1032, 1653, 1698
MslI	CAYNNNNRTG	4 cut(s)	49, 566, 1221, 1525
MspI	CCGG	3 cut(s)	174, 842, 996
MspR9I	CCNGG	7 cut(s)	174, 254, 314, 365, 737, 996, 1682
Mva1269I	GAATGC	1 cut(s)	838
MvaI	CCWGG	5 cut(s)	254, 314, 365, 737, 1682
MvnI	CGCG	8 cut(s)	226, 404, 480, 512, 623, 638, 839, 851
MwoI	GCNNNNNNNGC	7 cut(s)	218, 245, 615, 833, 1097, 1328, 1435
NciI	CCSGG	2 cut(s)	174, 996
NdeII	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
NheI	GCTAGC	1 cut(s)	208
NlaIV	GGNNCC	4 cut(s)	677, 678, 912, 1084
NmuCI	GTSAC	6 cut(s)	176, 265, 468, 778, 944, 1324
NsbI	TGCGCA	1 cut(s)	1353
NspI	RCATGY	1 cut(s)	1652
OliI	CACNNNNGTG	1 cut(s)	1525
PaeR7I	CTCGAG	2 cut(s)	290, 1474
PasI	CCCWGGG	1 cut(s)	253
PciSI	GCTCTTC	1 cut(s)	1515
PcsI	WCGNNNNNNNCGW	3 cut(s)	642, 900, 1143
PctI	GAATGC	1 cut(s)	838
PdmI	GAANNNNTTC	1 cut(s)	1509
PfeI	GAWTC	1 cut(s)	754
Pfl23II	CGTACG	1 cut(s)	642
PflFI	GACNNNGTC	1 cut(s)	583
PfoI	TCCNGGA	1 cut(s)	363
PkrI	GCNGC	5 cut(s)	56, 403, 511, 838, 1631
PleI	GAGTC	2 cut(s)	362, 590
PmaCI	CACGTG	2 cut(s)	473, 494
PmlI	CACGTG	2 cut(s)	473, 494
PpsI	GAGTC	2 cut(s)	362, 590
Ppu21I	YACGTR	3 cut(s)	473, 494, 1659
PpuMI	RGGWCCY	1 cut(s)	1082
PsiI	TTATAA	2 cut(s)	38, 1490
Psp5II	RGGWCCY	1 cut(s)	1082
Psp6I	CCWGG	5 cut(s)	252, 312, 363, 735, 1680
PspCI	CACGTG	2 cut(s)	473, 494
PspGI	CCWGG	5 cut(s)	252, 312, 363, 735, 1680
PspLI	CGTACG	1 cut(s)	642
PspN4I	GGNNCC	4 cut(s)	677, 678, 912, 1084
PspOMI	GGGCCC	1 cut(s)	676
PspPI	GGNCC	4 cut(s)	352, 676, 677, 1082
PspPPI	RGGWCCY	1 cut(s)	1082
PspXI	VCTCGAGB	1 cut(s)	1474
PstI	CTGCAG	1 cut(s)	1671
PstNI	CAGNNNCTG	2 cut(s)	1328, 1349
PsuI	RGATCY	2 cut(s)	201, 1635
PsyI	GACNNNGTC	1 cut(s)	583
RsaI	GTAC	6 cut(s)	527, 551, 644, 893, 1406, 1472
RsaNI	GTAC	6 cut(s)	526, 550, 643, 892, 1405, 1471
RseI	CAYNNNNRTG	4 cut(s)	49, 566, 1221, 1525
SapI	GCTCTTC	1 cut(s)	1515
SaqAI	TTAA	6 cut(s)	483, 767, 980, 1032, 1653, 1698
SatI	GCNGC	5 cut(s)	55, 402, 510, 837, 1630
Sau3AI	GATC	8 cut(s)	201, 392, 603, 633, 1112, 1123, 1386, 1635
Sau96I	GGNCC	4 cut(s)	352, 676, 677, 1082
ScaI	AGTACT	1 cut(s)	1472
SchI	GAGTC	2 cut(s)	362, 590
ScrFI	CCNGG	7 cut(s)	174, 254, 314, 365, 737, 996, 1682
SduI	GDGCHC	5 cut(s)	680, 915, 977, 1211, 1440
SfaNI	GCATC	2 cut(s)	1078, 1521
SfcI	CTRYAG	1 cut(s)	1667
Sfr274I	CTCGAG	2 cut(s)	290, 1474
SinI	GGWCC	2 cut(s)	352, 1082
SlaI	CTCGAG	2 cut(s)	290, 1474
SmiMI	CAYNNNNRTG	4 cut(s)	49, 566, 1221, 1525
SmlI	CTYRAG	3 cut(s)	290, 545, 1474
SmoI	CTYRAG	3 cut(s)	290, 545, 1474
SnaBI	TACGTA	1 cut(s)	1659
SspMI	CTAG	2 cut(s)	209, 1287
StyD4I	CCNGG	7 cut(s)	172, 252, 312, 363, 735, 994, 1680
StyI	CCWWGG	3 cut(s)	424, 1333, 1535
TaiI	ACGT	6 cut(s)	423, 475, 496, 780, 933, 1661
TaqI	TCGA	5 cut(s)	291, 417, 594, 1137, 1475
TatI	WGTACW	2 cut(s)	1404, 1470
TauI	GCSGC	3 cut(s)	404, 512, 839
TfiI	GAWTC	1 cut(s)	754
Tru1I	TTAA	6 cut(s)	483, 767, 980, 1032, 1653, 1698
Tru9I	TTAA	6 cut(s)	483, 767, 980, 1032, 1653, 1698
TscAI	CASTG	4 cut(s)	274, 1021, 1126, 1333
TseFI	GTSAC	6 cut(s)	176, 265, 468, 778, 944, 1324
TseI	GCWGC	2 cut(s)	54, 1629
Tsp45I	GTSAC	6 cut(s)	176, 265, 468, 778, 944, 1324
TspDTI	ATGAA	4 cut(s)	1036, 1141, 1222, 1424
TspGWI	ACGGA	5 cut(s)	704, 747, 1120, 1271, 1412
TspRI	CASTG	4 cut(s)	274, 1021, 1126, 1333
Tth111I	GACNNNGTC	1 cut(s)	583
VneI	GTGCAC	2 cut(s)	1207, 1436
VpaK11BI	GGWCC	2 cut(s)	352, 1082
XagI	CCTNNNNNAGG	1 cut(s)	257
XapI	RAATTY	8 cut(s)	32, 444, 796, 999, 1148, 1577, 1585, 1672
XceI	RCATGY	1 cut(s)	1652
XhoI	CTCGAG	2 cut(s)	290, 1474
XmnI	GAANNNNTTC	1 cut(s)	1509
XspI	CTAG	2 cut(s)	209, 1287
ZraI	GACGTC	1 cut(s)	421
ZrmI	AGTACT	1 cut(s)	1472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.