Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Reverse (-)

64140757 .. 64142400

Gene Structure 1644 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00271810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGTCTACCCGCGTCTTTCTCCTCACACTCGCTCTCTGCATCTCTCTTTCCTCCGCCTCCGCGGCCCGCTTCCGCTCCATCCCCAAGCCCACAGCCACCGCCGCCGTATCTCCGATCCAACAAGCCTGCAAAGCCACCCGCTTCCCTGACACATGTCAGGCCTCCCTGACCAAAAAAATTCATGACCTTCCCCCAAACCCGACCCCTCTGCAAACACTTCAACTAGCCCTCAATGTCTCCTCCGACGGCCTCAACACAGCCAAAAAGATGGTTTCCGACATTCTCAAAACCGCCGCCGGCAGCCAGAACCGCACAACCGCCGCCAAAAACTGCCTCGATTTTCTACAAAATTCCGAGTACCGTATTTCACTCACCAATGACGGCCTCTCACGTGGCCGTATCAAGACCGCACGTGCCGCCATGAGCTCCGCCCTGATGTACCAGTACGACTGCTGGTCCGCATTCAAGTACGCCAACGACACCCAGTTGGTCGTCTCCACCATGTCGTTCATTAACGACACGCTGACCGTGGACTCCAGCAACGCCCTTAGCATGATCTTCTCCTACGAAAACTTCGGAAACGACACCAAGTCCTGGACTCCGCCCAAGACCGAGCGCGACGGGTTCTGGGAACCTGTCACGCCCGGATCACAAAAGTCCCCTTCTGAACTTTCGATACCGAAAAATCTGATGGCGGACGTGACTGTTTGCAAGGACAAAGGTGTTAAGTGTTACCGGACCATACAGGAGGCGGTGAACGCGGCGCCGGAAAACTCCGACAAGAAATTCGTGATTCAGATAAATGCCGGCGTGTATGAAGAGATTGTTAAGGTTCCTCTAGGGAAGAGAAATGTTGTGTTTTTAGGGCAAGGGATGGGCAAAACAGTAATTACAGGATCTTTGTATGTGGGCCAGCCGGGAATTTCGACCTACAACTCCGCCACCGTGGGTGTTTACGGCGACGGCTTCATGGCCAGAGGCGTCACATTCCAAAACACGGCGGGGGCTGACGCCCAGCAGGAGGTTCACCAGGCGGTGGCTTTCCGATCAGACAGTGATCACTCTATCATCGAAAATTGTGAGTTCCTAGGGAACCAAGACACTCTCTACGCCCACGGCAACCGCCAGTTCTACAAGTCATGCAAAATTCAAGGCAATGTAGATTTTATATTTGGAAACTCTGCTGCCATTTTCGAGGGCTGCACCATCCTCATTGCGCCGAGGCAAGTAAACCCCGAAAAAGGCGAGAAGAATGCCGTGACGGCTCACGGCAGGACGGACCCGGCGCAGGCGACAGGGTTCGTGTTCCGGAACTGCTCCATAAGCGGAACGGAAGAGTACATGAAGTTGTACAACATTAACCCCAAAGTGCACTTGAACTTCTTGGGGAGGCCGTGGAAGGAGTTTTCGAGGACGGTGTTCTTGAACTGTAGTATGGAAGCTCTGGTTTCGAAAGAAGGGTGGTTGCCTTGGAGTGAGGAGTTTGCTTTGGCGACGCTTTACTATGGGGAGAATGGGAACTCGGGACCGGGGGCTGATTTGGCCGGGAGGGTGAAGTGGAGTAGCCGGATTCCGGTAGAGCATGTTGGTGCATATTCGGTGCAAAATTTTATTCAGGGAGATGAGTGGTTGGTTTCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

547

Amino Acids

59.83

Weight (kDa)

8.32

Isoelectric Point (pI)

30.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	39 - 185	5.2e-20	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	233 - 541	3e-111	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	763
AccB7I	CCANNNNNTGG	2 cut(s)	594, 1036
AccBSI	CCGCTC	1 cut(s)	75
AccI	GTMKAC	1 cut(s)	5
AccII	CGCG	4 cut(s)	12, 62, 618, 761
AccIII	TCCGGA	1 cut(s)	1308
AclWI	GGATC	3 cut(s)	109, 655, 904
AcoI	YGGCCR	3 cut(s)	394, 972, 1542
AcsI	RAATTY	6 cut(s)	177, 349, 785, 921, 1146, 1606
AcvI	CACGTG	2 cut(s)	392, 413
AcyI	GRCGYC	3 cut(s)	764, 981, 1011
AfaI	GTAC	6 cut(s)	359, 440, 446, 470, 1340, 1352
AfiI	CCNNNNNNNGG	7 cut(s)	594, 997, 1021, 1036, 1241, 1288, 1573
AflIII	ACRYGT	1 cut(s)	152
AgsI	TTSAA	5 cut(s)	221, 466, 1151, 1378, 1426
AhdI	GACNNNNNGTC	2 cut(s)	454, 589
AjiI	CACGTC	1 cut(s)	700
AjnI	CCWGG	2 cut(s)	593, 1029
AluBI	AGCT	2 cut(s)	426, 1442
AluI	AGCT	2 cut(s)	426, 1442
Alw21I	GWGCWC	2 cut(s)	428, 1374
Alw26I	GTCTC	2 cut(s)	241, 499
Alw44I	GTGCAC	1 cut(s)	1370
AlwI	GGATC	3 cut(s)	109, 655, 904
Ama87I	CYCGRG	1 cut(s)	1522
Aor13HI	TCCGGA	1 cut(s)	1308
AoxI	GGCC	9 cut(s)	63, 159, 247, 382, 394, 910, 972, 1391, 1542
ApaLI	GTGCAC	1 cut(s)	1370
ApeKI	GCWGC	3 cut(s)	300, 1184, 1200
ApoI	RAATTY	6 cut(s)	177, 349, 785, 921, 1146, 1606
ArsI	GACNNNNNNTTYG	2 cut(s)	770, 802
AspA2I	CCTAGG	1 cut(s)	1087
AspLEI	GCGC	4 cut(s)	618, 766, 1219, 1288
AspS9I	GGNCC	6 cut(s)	64, 456, 738, 910, 1279, 1526
AsuC2I	CCSGG	5 cut(s)	645, 918, 1283, 1530, 1546
AsuHPI	GGTGA	4 cut(s)	364, 766, 1019, 1564
AsuII	TTCGAA	1 cut(s)	1451
AvaI	CYCGRG	1 cut(s)	1522
AvaII	GGWCC	4 cut(s)	456, 738, 1279, 1526
AvrII	CCTAGG	1 cut(s)	1087
BaeGI	GKGCMC	1 cut(s)	1374
BalI	TGGCCA	1 cut(s)	974
BanI	GGYRCC	1 cut(s)	763
BanII	GRGCYC	1 cut(s)	428
BbrPI	CACGTG	2 cut(s)	392, 413
Bbv12I	GWGCWC	2 cut(s)	428, 1374
BbvI	GCAGC	3 cut(s)	312, 1171, 1187
BccI	CCATC	5 cut(s)	86, 262, 685, 868, 1214
BcgI	CGANNNNNNTGC	4 cut(s)	190, 224, 1235, 1269
BciT130I	CCWGG	2 cut(s)	595, 1031
BclI	TGATCA	1 cut(s)	1057
BcnI	CCSGG	5 cut(s)	645, 918, 1283, 1530, 1546
BcoDI	GTCTC	2 cut(s)	241, 499
BfaI	CTAG	4 cut(s)	224, 839, 1088, 1642
BfmI	CTRYAG	1 cut(s)	1429
BfoI	RGCGCY	1 cut(s)	767
BglI	GCCNNNNNGGC	2 cut(s)	62, 1262
BisI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1185, 1201
BlnI	CCTAGG	1 cut(s)	1087
BlsI	GCNGC	9 cut(s)	64, 103, 295, 302, 322, 418, 763, 1186, 1202
Bme1390I	CCNGG	7 cut(s)	595, 645, 918, 1031, 1283, 1530, 1546
Bme18I	GGWCC	4 cut(s)	456, 738, 1279, 1526
BmeRI	GACNNNNNGTC	2 cut(s)	454, 589
BmeT110I	CYCGRG	1 cut(s)	1522
BmgBI	CACGTC	1 cut(s)	700
BmgT120I	GGNCC	6 cut(s)	64, 456, 738, 910, 1279, 1526
BmiI	GGNNCC	6 cut(s)	633, 765, 834, 1094, 1281, 1527
BmrFI	CCNGG	7 cut(s)	595, 645, 918, 1031, 1283, 1530, 1546
BmrI	ACTGGG	1 cut(s)	478
BmsI	GCATC	1 cut(s)	48
BmuI	ACTGGG	1 cut(s)	478
BoxI	GACNNNNGTC	1 cut(s)	153
BpmI	CTGGAG	1 cut(s)	520
Bpu10I	CCTNAGC	1 cut(s)	548
Bpu14I	TTCGAA	1 cut(s)	1451
BpuMI	CCSGG	5 cut(s)	645, 918, 1283, 1530, 1546
BsaAI	YACGTR	2 cut(s)	392, 413
BsaHI	GRCGYC	3 cut(s)	764, 981, 1011
BsaJI	CCNNGG	9 cut(s)	60, 528, 945, 1087, 1114, 1220, 1394, 1469, 1529
BsaWI	WCCGGW	3 cut(s)	735, 1308, 1573
Bsc4I	CCNNNNNNNGG	7 cut(s)	594, 997, 1021, 1036, 1241, 1288, 1573
Bse118I	RCCGGY	2 cut(s)	296, 806
Bse1I	ACTGG	3 cut(s)	442, 484, 1126
Bse3DI	GCAATG	2 cut(s)	1162, 1212
BseAI	TCCGGA	1 cut(s)	1308
BseBI	CCWGG	2 cut(s)	595, 1031
BseDI	CCNNGG	9 cut(s)	60, 528, 945, 1087, 1114, 1220, 1394, 1469, 1529
BseGI	GGATG	3 cut(s)	78, 879, 1206
BseLI	CCNNNNNNNGG	7 cut(s)	594, 997, 1021, 1036, 1241, 1288, 1573
BseMI	GCAATG	2 cut(s)	1162, 1212
BseNI	ACTGG	3 cut(s)	442, 484, 1126
BseRI	GAGGAG	3 cut(s)	11, 229, 1493
BseSI	GKGCMC	1 cut(s)	1374
BseXI	GCAGC	3 cut(s)	312, 1171, 1187
BseYI	CCCAGC	1 cut(s)	1014
BsgI	GTGCAG	1 cut(s)	1186
Bsh1236I	CGCG	4 cut(s)	12, 62, 618, 761
BshFI	GGCC	9 cut(s)	65, 161, 249, 384, 396, 912, 974, 1393, 1544
BshNI	GGYRCC	1 cut(s)	763
BsiHKAI	GWGCWC	2 cut(s)	428, 1374
BsiHKCI	CYCGRG	1 cut(s)	1522
BslFI	GGGAC	2 cut(s)	643, 1539
BslI	CCNNNNNNNGG	7 cut(s)	594, 997, 1021, 1036, 1241, 1288, 1573
BsmAI	GTCTC	2 cut(s)	241, 499
BsmBI	CGTCTC	1 cut(s)	499
BsmFI	GGGAC	2 cut(s)	643, 1539
BsmI	GAATGC	2 cut(s)	461, 1258
BsnI	GGCC	9 cut(s)	65, 161, 249, 384, 396, 912, 974, 1393, 1544
BsoBI	CYCGRG	1 cut(s)	1522
Bsp119I	TTCGAA	1 cut(s)	1451
Bsp1286I	GDGCHC	2 cut(s)	428, 1374
Bsp13I	TCCGGA	1 cut(s)	1308
Bsp1407I	TGTACA	1 cut(s)	1350
Bsp143I	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
BspANI	GGCC	9 cut(s)	65, 161, 249, 384, 396, 912, 974, 1393, 1544
BspEI	TCCGGA	1 cut(s)	1308
BspFNI	CGCG	4 cut(s)	12, 62, 618, 761
BspHI	TCATGA	1 cut(s)	181
BspLI	GGNNCC	6 cut(s)	633, 765, 834, 1094, 1281, 1527
BspPI	GGATC	3 cut(s)	109, 655, 904
BspT104I	TTCGAA	1 cut(s)	1451
BspT107I	GGYRCC	1 cut(s)	763
BsrBI	CCGCTC	1 cut(s)	75
BsrDI	GCAATG	2 cut(s)	1162, 1212
BsrFI	RCCGGY	2 cut(s)	296, 806
BsrGI	TGTACA	1 cut(s)	1350
BsrI	ACTGG	3 cut(s)	442, 484, 1126
BssAI	RCCGGY	2 cut(s)	296, 806
BssECI	CCNNGG	9 cut(s)	60, 528, 945, 1087, 1114, 1220, 1394, 1469, 1529
BssMI	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
BssNI	GRCGYC	3 cut(s)	764, 981, 1011
BssT1I	CCWWGG	2 cut(s)	1087, 1469
Bst2UI	CCWGG	2 cut(s)	595, 1031
Bst4CI	ACNGT	8 cut(s)	362, 529, 706, 886, 946, 1055, 1417, 1430
Bst6I	CTCTTC	3 cut(s)	813, 839, 1329
BstACI	GRCGYC	3 cut(s)	764, 981, 1011
BstAUI	TGTACA	1 cut(s)	1350
BstBAI	YACGTR	2 cut(s)	392, 413
BstBI	TTCGAA	1 cut(s)	1451
BstC8I	GCNNGC	6 cut(s)	67, 127, 298, 808, 914, 1290
BstDEI	CTNAG	1 cut(s)	548
BstDSI	CCRYGG	5 cut(s)	60, 528, 945, 1114, 1394
BstF5I	GGATG	3 cut(s)	78, 879, 1206
BstFNI	CGCG	4 cut(s)	12, 62, 618, 761
BstH2I	RGCGCY	1 cut(s)	767
BstHHI	GCGC	4 cut(s)	618, 766, 1219, 1288
BstKTI	GATC	6 cut(s)	117, 558, 650, 899, 1049, 1060
BstMAI	GTCTC	2 cut(s)	241, 499
BstMBI	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
BstMWI	GCNNNNNNNGC	9 cut(s)	62, 101, 131, 309, 416, 758, 1262, 1323, 1541
BstNI	CCWGG	2 cut(s)	595, 1031
BstNSI	RCATGY	2 cut(s)	156, 1586
BstPAI	GACNNNNGTC	1 cut(s)	153
BstSCI	CCNGG	7 cut(s)	593, 643, 916, 1029, 1281, 1528, 1544
BstSFI	CTRYAG	1 cut(s)	1429
BstSLI	GKGCMC	1 cut(s)	1374
BstUI	CGCG	4 cut(s)	12, 62, 618, 761
BstV1I	GCAGC	3 cut(s)	312, 1171, 1187
BstX2I	RGATCY	1 cut(s)	896
BstXI	CCANNNNNNTGG	1 cut(s)	268
BstYI	RGATCY	1 cut(s)	896
BsuRI	GGCC	9 cut(s)	65, 161, 249, 384, 396, 912, 974, 1393, 1544
BtgI	CCRYGG	5 cut(s)	60, 528, 945, 1114, 1394
BtrI	CACGTC	1 cut(s)	700
BtsCI	GGATG	3 cut(s)	78, 879, 1206
BtsIMutI	CAGTG	1 cut(s)	1060
Cac8I	GCNNGC	6 cut(s)	67, 127, 298, 808, 914, 1290
CciI	TCATGA	1 cut(s)	181
CfoI	GCGC	4 cut(s)	618, 766, 1219, 1288
Cfr10I	RCCGGY	2 cut(s)	296, 806
Cfr13I	GGNCC	6 cut(s)	64, 456, 738, 910, 1279, 1526
Cfr42I	CCGCGG	1 cut(s)	63
CseI	GACGC	3 cut(s)	970, 1019, 1504
Csp6I	GTAC	6 cut(s)	358, 439, 445, 469, 1339, 1351
CviAII	CATG	9 cut(s)	153, 182, 421, 502, 553, 970, 1140, 1342, 1583
CviQI	GTAC	6 cut(s)	358, 439, 445, 469, 1339, 1351
DdeI	CTNAG	1 cut(s)	548
DinI	GGCGCC	1 cut(s)	765
DpnI	GATC	6 cut(s)	116, 557, 649, 898, 1048, 1059
DpnII	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
DriI	GACNNNNNGTC	2 cut(s)	454, 589
EaeI	YGGCCR	3 cut(s)	394, 972, 1542
Eam1104I	CTCTTC	3 cut(s)	813, 839, 1329
Eam1105I	GACNNNNNGTC	2 cut(s)	454, 589
EarI	CTCTTC	3 cut(s)	813, 839, 1329
EciI	GGCGGA	5 cut(s)	43, 418, 591, 710, 928
Ecl136II	GAGCTC	1 cut(s)	426
Eco130I	CCWWGG	2 cut(s)	1087, 1469
Eco147I	AGGCCT	1 cut(s)	161
Eco24I	GRGCYC	1 cut(s)	428
Eco47I	GGWCC	4 cut(s)	456, 738, 1279, 1526
Eco53kI	GAGCTC	1 cut(s)	426
Eco72I	CACGTG	2 cut(s)	392, 413
Eco88I	CYCGRG	1 cut(s)	1522
EcoICRI	GAGCTC	1 cut(s)	426
EcoRII	CCWGG	2 cut(s)	593, 1029
EcoT14I	CCWWGG	2 cut(s)	1087, 1469
EcoT38I	GRGCYC	1 cut(s)	428
EgeI	GGCGCC	1 cut(s)	765
EheI	GGCGCC	1 cut(s)	765
ErhI	CCWWGG	2 cut(s)	1087, 1469
Esp3I	CGTCTC	1 cut(s)	499
FaeI	CATG	9 cut(s)	156, 185, 424, 505, 556, 973, 1143, 1345, 1586
FaqI	GGGAC	2 cut(s)	643, 1539
FatI	CATG	9 cut(s)	152, 181, 420, 501, 552, 969, 1139, 1341, 1582
FauI	CCCGC	4 cut(s)	17, 74, 146, 994
FbaI	TGATCA	1 cut(s)	1057
FblI	GTMKAC	1 cut(s)	5
Fnu4HI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1185, 1201
FokI	GGATG	3 cut(s)	65, 886, 1193
FriOI	GRGCYC	1 cut(s)	428
Fsp4HI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1185, 1201
FspBI	CTAG	4 cut(s)	224, 839, 1088, 1642
GlaI	GCGC	4 cut(s)	617, 765, 1218, 1287
GluI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1185, 1201
GsaI	CCCAGC	1 cut(s)	1018
GsuI	CTGGAG	1 cut(s)	520
HaeII	RGCGCY	1 cut(s)	767
HaeIII	GGCC	9 cut(s)	65, 161, 249, 384, 396, 912, 974, 1393, 1544
HgaI	GACGC	3 cut(s)	970, 1019, 1504
HhaI	GCGC	4 cut(s)	618, 766, 1219, 1288
Hin1I	GRCGYC	3 cut(s)	764, 981, 1011
Hin1II	CATG	9 cut(s)	156, 185, 424, 505, 556, 973, 1143, 1345, 1586
Hin6I	GCGC	4 cut(s)	616, 764, 1217, 1286
HinP1I	GCGC	4 cut(s)	616, 764, 1217, 1286
HinfI	GANTC	4 cut(s)	533, 598, 793, 1570
HphI	GGTGA	4 cut(s)	364, 766, 1019, 1564
Hpy166II	GTNNAC	7 cut(s)	6, 532, 757, 955, 1027, 1231, 1372
Hpy188III	TCNNGA	6 cut(s)	182, 403, 790, 1309, 1423, 1524
Hpy8I	GTNNAC	7 cut(s)	6, 532, 757, 955, 1027, 1231, 1372
Hpy99I	CGWCG	4 cut(s)	248, 623, 965, 1498
HpyAV	CCTTC	4 cut(s)	197, 672, 1393, 1451
HpyCH4III	ACNGT	8 cut(s)	362, 529, 706, 886, 946, 1055, 1417, 1430
HpyCH4IV	ACGT	3 cut(s)	391, 412, 699
HpyCH4V	TGCA	9 cut(s)	39, 129, 211, 711, 1143, 1203, 1372, 1592, 1603
HpyF10VI	GCNNNNNNNGC	9 cut(s)	62, 101, 131, 309, 416, 758, 1262, 1323, 1541
HpyF3I	CTNAG	1 cut(s)	548
HpySE526I	ACGT	3 cut(s)	391, 412, 699
Hsp92I	GRCGYC	3 cut(s)	764, 981, 1011
Hsp92II	CATG	9 cut(s)	156, 185, 424, 505, 556, 973, 1143, 1345, 1586
HspAI	GCGC	4 cut(s)	616, 764, 1217, 1286
KasI	GGCGCC	1 cut(s)	763
Kpn2I	TCCGGA	1 cut(s)	1308
KroI	GCCGGC	2 cut(s)	296, 806
KroNI	GCCGGC	2 cut(s)	298, 808
Ksp22I	TGATCA	1 cut(s)	1057
KspI	CCGCGG	1 cut(s)	63
Kzo9I	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
LmnI	GCTCC	3 cut(s)	80, 431, 1322
Lsp1109I	GCAGC	3 cut(s)	312, 1171, 1187
LweI	GCATC	1 cut(s)	48
MaeI	CTAG	4 cut(s)	224, 839, 1088, 1642
MaeII	ACGT	3 cut(s)	391, 412, 699
MaeIII	GTNAC	5 cut(s)	637, 700, 731, 982, 1258
MalI	GATC	6 cut(s)	116, 557, 649, 898, 1048, 1059
MbiI	CCGCTC	1 cut(s)	75
MboI	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
MboII	GAAGA	5 cut(s)	550, 830, 856, 1261, 1346
MflI	RGATCY	1 cut(s)	896
MhlI	GDGCHC	2 cut(s)	428, 1374
MlsI	TGGCCA	1 cut(s)	974
MluCI	AATT	8 cut(s)	177, 349, 785, 888, 921, 1075, 1146, 1606
MluNI	TGGCCA	1 cut(s)	974
Mly113I	GGCGCC	1 cut(s)	764
MlyI	GAGTC	2 cut(s)	527, 592
MmeI	TCCRAC	4 cut(s)	142, 267, 300, 801
Mox20I	TGGCCA	1 cut(s)	974
MroI	TCCGGA	1 cut(s)	1308
MroNI	GCCGGC	2 cut(s)	296, 806
MscI	TGGCCA	1 cut(s)	974
MseI	TTAA	4 cut(s)	513, 726, 828, 1359
MslI	CAYNNNNRTG	1 cut(s)	1587
Msp20I	TGGCCA	1 cut(s)	974
MspA1I	CMGCKG	1 cut(s)	62
MspR9I	CCNGG	7 cut(s)	595, 645, 918, 1031, 1283, 1530, 1546
Mva1269I	GAATGC	2 cut(s)	461, 1258
MvaI	CCWGG	2 cut(s)	595, 1031
MvnI	CGCG	4 cut(s)	12, 62, 618, 761
MwoI	GCNNNNNNNGC	9 cut(s)	62, 101, 131, 309, 416, 758, 1262, 1323, 1541
NaeI	GCCGGC	2 cut(s)	298, 808
NarI	GGCGCC	1 cut(s)	764
NciI	CCSGG	5 cut(s)	645, 918, 1283, 1530, 1546
NdeII	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
NgoMIV	GCCGGC	2 cut(s)	296, 806
NlaIII	CATG	9 cut(s)	156, 185, 424, 505, 556, 973, 1143, 1345, 1586
NlaIV	GGNNCC	6 cut(s)	633, 765, 834, 1094, 1281, 1527
NmeAIII	GCCGAG	1 cut(s)	1245
NmuCI	GTSAC	4 cut(s)	637, 700, 982, 1258
NspI	RCATGY	2 cut(s)	156, 1586
NspV	TTCGAA	1 cut(s)	1451
PagI	TCATGA	1 cut(s)	181
PceI	AGGCCT	1 cut(s)	161
PciI	ACATGT	1 cut(s)	152
PctI	GAATGC	2 cut(s)	461, 1258
PdiI	GCCGGC	2 cut(s)	298, 808
PfeI	GAWTC	2 cut(s)	793, 1570
PflMI	CCANNNNNTGG	2 cut(s)	594, 1036
PfoI	TCCNGGA	1 cut(s)	593
PkrI	GCNGC	9 cut(s)	64, 103, 295, 302, 322, 418, 763, 1186, 1202
PleI	GAGTC	2 cut(s)	527, 592
PluTI	GGCGCC	1 cut(s)	767
PmaCI	CACGTG	2 cut(s)	392, 413
PmlI	CACGTG	2 cut(s)	392, 413
PpsI	GAGTC	2 cut(s)	527, 592
Ppu21I	YACGTR	2 cut(s)	392, 413
PscI	ACATGT	1 cut(s)	152
PshAI	GACNNNNGTC	1 cut(s)	153
Psp124BI	GAGCTC	1 cut(s)	428
Psp6I	CCWGG	2 cut(s)	593, 1029
PspCI	CACGTG	2 cut(s)	392, 413
PspFI	CCCAGC	1 cut(s)	1014
PspGI	CCWGG	2 cut(s)	593, 1029
PspN4I	GGNNCC	6 cut(s)	633, 765, 834, 1094, 1281, 1527
PspPI	GGNCC	6 cut(s)	64, 456, 738, 910, 1279, 1526
PsrI	GAACNNNNNNTAC	2 cut(s)	660, 692
PsuI	RGATCY	1 cut(s)	896
RsaI	GTAC	6 cut(s)	359, 440, 446, 470, 1340, 1352
RsaNI	GTAC	6 cut(s)	358, 439, 445, 469, 1339, 1351
RseI	CAYNNNNRTG	1 cut(s)	1587
SacI	GAGCTC	1 cut(s)	428
SacII	CCGCGG	1 cut(s)	63
SaqAI	TTAA	4 cut(s)	513, 726, 828, 1359
SatI	GCNGC	9 cut(s)	63, 102, 294, 301, 321, 417, 762, 1185, 1201
Sau3AI	GATC	6 cut(s)	114, 555, 647, 896, 1046, 1057
Sau96I	GGNCC	6 cut(s)	64, 456, 738, 910, 1279, 1526
SchI	GAGTC	2 cut(s)	527, 592
ScrFI	CCNGG	7 cut(s)	595, 645, 918, 1031, 1283, 1530, 1546
SduI	GDGCHC	2 cut(s)	428, 1374
SfaNI	GCATC	1 cut(s)	48
SfcI	CTRYAG	1 cut(s)	1429
SfoI	GGCGCC	1 cut(s)	765
Sfr303I	CCGCGG	1 cut(s)	63
SfuI	TTCGAA	1 cut(s)	1451
SgrBI	CCGCGG	1 cut(s)	63
SinI	GGWCC	4 cut(s)	456, 738, 1279, 1526
SmiMI	CAYNNNNRTG	1 cut(s)	1587
Sse9I	AATT	8 cut(s)	177, 349, 785, 888, 921, 1075, 1146, 1606
SseBI	AGGCCT	1 cut(s)	161
SspDI	GGCGCC	1 cut(s)	763
SspMI	CTAG	4 cut(s)	224, 839, 1088, 1642
SstI	GAGCTC	1 cut(s)	428
StuI	AGGCCT	1 cut(s)	161
StyD4I	CCNGG	7 cut(s)	593, 643, 916, 1029, 1281, 1528, 1544
StyI	CCWWGG	2 cut(s)	1087, 1469
TaaI	ACNGT	8 cut(s)	362, 529, 706, 886, 946, 1055, 1417, 1430
TaiI	ACGT	3 cut(s)	394, 415, 702
TaqI	TCGA	7 cut(s)	336, 674, 926, 1071, 1194, 1409, 1451
TaqII	GACCGA	1 cut(s)	626
TasI	AATT	8 cut(s)	177, 349, 785, 888, 921, 1075, 1146, 1606
TatI	WGTACW	2 cut(s)	1338, 1350
TauI	GCSGC	6 cut(s)	65, 104, 296, 323, 419, 764
TfiI	GAWTC	2 cut(s)	793, 1570
Tru1I	TTAA	4 cut(s)	513, 726, 828, 1359
Tru9I	TTAA	4 cut(s)	513, 726, 828, 1359
TscAI	CASTG	1 cut(s)	1060
TseFI	GTSAC	4 cut(s)	637, 700, 982, 1258
TseI	GCWGC	3 cut(s)	300, 1184, 1200
Tsp45I	GTSAC	4 cut(s)	637, 700, 982, 1258
TspDTI	ATGAA	5 cut(s)	170, 499, 831, 958, 1358
TspGWI	ACGGA	2 cut(s)	1292, 1346
TspRI	CASTG	1 cut(s)	1060
Van91I	CCANNNNNTGG	2 cut(s)	594, 1036
VneI	GTGCAC	1 cut(s)	1370
VpaK11BI	GGWCC	4 cut(s)	456, 738, 1279, 1526
XapI	RAATTY	6 cut(s)	177, 349, 785, 921, 1146, 1606
XceI	RCATGY	2 cut(s)	156, 1586
XmaJI	CCTAGG	1 cut(s)	1087
XmiI	GTMKAC	1 cut(s)	5
XspI	CTAG	4 cut(s)	224, 839, 1088, 1642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.