Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Reverse (-)

28663740 .. 28665471

Gene Structure 1732 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1238800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGGCAATGCTGTTCTTCCTCCTCATTGTCCTCATGGCTTCCCTCCTCTTCCTCTCTCTCCTCTCCCTCACTCTCTTCCTCTCTCTCTCCTCCGCCCAGCACCACCACCACCACACTCACCGCCTCCTCACCTCCCCACAACCTCACATCCAACAAGCCTGCAAAGCCACGCGCTTCCCCGACGCCTGTCAAGCCTCCTTGACCAACCTCGTCACTGAACCCACCACCACCCCACTCCAAACCATCCAATTCGCCGTTCAACTCTCCGGCGACGGCGTCAAGACGGCGCAGGGAATGGTCAAGACTATCCTGGACTCGTCCCCGGCCAGCCACAACCGCACGACCGCCGCGAATAACTGCCTCGACATCCTCGCTAACTCCCAGTACCGAATCTCTCTCGCGACCGACAGTCTCTCACGTGGTAAAATTAAAAATGCACGTGCTTCTATGAGCTCCGCATTGCTCTACCAGTACGGGTGCTGGTCGGGCCTCAAGAACGCCAACGACACCAGCATGGTCGACGAGACGATGTCATTTCTCGACAGTTTGATCGGCAAATCCAGCAACGCGCTCGGCATGATGGCCTCGTACGACAACTTCGGCAACGACACAAAAATGTGGGCCCCGCCGAAGACGGAGCGAGACGGGTTCTGGGAGCGGCTGGAGCACGGAGGTCCGGACCCGGGATTTCGAGGTGGGGTGCCGTCGGGTTCAACGGCGAACGTGACGGTGTGCAAGGAGGAATGGTGTAATTACAGGACGGTGCAGGAGGCGGTGAATGCCGCACCAGATAACGCGGGAAACAAAAGGTTCGTGATTGGGATAAAGGGCGGGGTGTACGAGGAGACCGTGAGGGTGGCCTTAGAGAAGCGGAACGTGATGCTTTTGGGTGACGGGATCGGCAAAACGATCATTACCGGGTCGTTGAATGTCGGCCAGCCCGGAATTTCCACCTACAACACTGCCACCGTCGGGGTTCTTGGTGATGGGTTCATGGCAAGCGGGCTCACAATCCAGAACACAGCAGGTCCCGACGCCCGCCAAGCAGTTGCTTTCAGATCCGACAGCGACCTCTCGGTAATCGAAAACTGCGAATTCATAGGCAACCAGGACACTCTTTACGCACAAGGAAACCGACAGTTCTATAAGTTGTGCACCATCCATGGCAACGTCGATTTCATCTTCGGAAAGTCAGCTTCCATCTTCCAAGACTGCACCATCCTTGTCCGTCCGCGGCAACTCCTACCCGAGAAAGGCGAAGACAATGTCGTCACAGCCCACGGAAGAACAGACCCTGCGCTGGCAACGGGTTTCGTGTTTCGAAACTGCTCGATCAACGGCACGGAAGAGTACATGAGGTTGTACCGGAGCAAGCCGCAGGTGCACAAGAACTACTTGGGGAGGCCGTGGAAGGAGTATTCGAGGACGGATTTTATAAACTGCAGTATGGAAGCTCTTATTACACCCCAAGGGTGGATGCCTTGGAGTGGGGATTTCGCTCTCACCACCCTTTATCTTGGGGAGTTTGGGAATTCTGGGGCGGGTTGTGATTTGTCCCAGAGATGGAACTGGACTAGCAAGATCCCATCTCAACATGTTAATACGTATTCTGTGCAGAATTTTATTCAGGGAGATGAGTGGATGTCAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

551

Amino Acids

60.35

Weight (kDa)

6.67

Isoelectric Point (pI)

35.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	50 - 192	3.1e-18	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	241 - 545	1.4e-110	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1436
AarI	CACCTGC	1 cut(s)	1369
AasI	GACNNNNNNGTC	1 cut(s)	1268
Acc36I	ACCTGC	2 cut(s)	1016, 1369
AccB1I	GGYRCC	1 cut(s)	700
AccBSI	CCGCTC	1 cut(s)	658
AccI	GTMKAC	1 cut(s)	519
AccII	CGCG	6 cut(s)	172, 350, 401, 569, 797, 1234
AccIII	TCCGGA	1 cut(s)	676
AclWI	GGATC	3 cut(s)	905, 1053, 1576
AcoI	YGGCCR	2 cut(s)	324, 934
AcsI	RAATTY	4 cut(s)	945, 1094, 1531, 1618
AcvI	CACGTG	2 cut(s)	419, 440
AcyI	GRCGYC	3 cut(s)	183, 276, 1035
AfaI	GTAC	6 cut(s)	386, 473, 590, 839, 1352, 1364
AfiI	CCNNNNNNNGG	7 cut(s)	683, 972, 1253, 1300, 1473, 1487, 1563
AflIII	ACRYGT	1 cut(s)	1594
AgsI	TTSAA	3 cut(s)	260, 714, 928
AhdI	GACNNNNNGTC	1 cut(s)	408
AjnI	CCWGG	2 cut(s)	309, 1107
AjuI	GAANNNNNNNTTGG	2 cut(s)	240, 272
AluBI	AGCT	3 cut(s)	453, 1196, 1454
AluI	AGCT	3 cut(s)	453, 1196, 1454
Alw21I	GWGCWC	4 cut(s)	455, 669, 1157, 1386
Alw26I	GTCTC	4 cut(s)	416, 518, 636, 839
Alw44I	GTGCAC	2 cut(s)	1153, 1382
AlwI	GGATC	3 cut(s)	905, 1053, 1576
AlwNI	CAGNNNCTG	1 cut(s)	1295
Ama87I	CYCGRG	2 cut(s)	682, 1247
Aor13HI	TCCGGA	1 cut(s)	676
AoxI	GGCC	7 cut(s)	324, 487, 582, 621, 858, 934, 1403
ApaI	GGGCCC	1 cut(s)	625
ApaLI	GTGCAC	2 cut(s)	1153, 1382
ApoI	RAATTY	4 cut(s)	945, 1094, 1531, 1618
ArsI	GACNNNNNNTTYG	2 cut(s)	1251, 1283
AspLEI	GCGC	4 cut(s)	174, 289, 571, 1300
AspS9I	GGNCC	6 cut(s)	487, 621, 622, 674, 679, 1028
AsuC2I	CCSGG	5 cut(s)	323, 683, 684, 919, 942
AsuHPI	GGTGA	6 cut(s)	110, 121, 787, 902, 995, 1495
AsuII	TTCGAA	1 cut(s)	1321
AvaI	CYCGRG	2 cut(s)	682, 1247
AvaII	GGWCC	3 cut(s)	674, 679, 1028
BaeGI	GKGCMC	3 cut(s)	625, 1157, 1386
BanI	GGYRCC	1 cut(s)	700
BanII	GRGCYC	3 cut(s)	455, 625, 1008
BarI	GAAGNNNNNNTAC	2 cut(s)	581, 613
BbrPI	CACGTG	2 cut(s)	419, 440
BbsI	GAAGAC	2 cut(s)	638, 1266
Bbv12I	GWGCWC	4 cut(s)	455, 669, 1157, 1386
BccI	CCATC	8 cut(s)	251, 574, 980, 1166, 1208, 1226, 1557, 1594
BceAI	ACGGC	7 cut(s)	239, 289, 300, 688, 732, 1354, 1390
BcgI	CGANNNNNNTGC	2 cut(s)	553, 587
BciT130I	CCWGG	2 cut(s)	311, 1109
BcnI	CCSGG	5 cut(s)	323, 683, 684, 919, 942
BcoDI	GTCTC	4 cut(s)	416, 518, 636, 839
BfaI	CTAG	1 cut(s)	1575
BfmI	CTRYAG	1 cut(s)	1441
BfuAI	ACCTGC	2 cut(s)	1016, 1369
BisI	GCNGC	5 cut(s)	348, 659, 783, 1235, 1376
BlsI	GCNGC	5 cut(s)	349, 660, 784, 1236, 1377
Bme1390I	CCNGG	7 cut(s)	311, 323, 683, 684, 919, 942, 1109
Bme18I	GGWCC	3 cut(s)	674, 679, 1028
BmeRI	GACNNNNNGTC	1 cut(s)	408
BmeT110I	CYCGRG	2 cut(s)	682, 1247
BmgT120I	GGNCC	6 cut(s)	487, 621, 622, 674, 679, 1028
BmiI	GGNNCC	5 cut(s)	623, 624, 681, 702, 1030
BmrFI	CCNGG	7 cut(s)	311, 323, 683, 684, 919, 942, 1109
BmrI	ACTGGG	1 cut(s)	376
BmsI	GCATC	2 cut(s)	870, 1467
BmuI	ACTGGG	1 cut(s)	376
BoxI	GACNNNNGTC	1 cut(s)	186
BpiI	GAAGAC	2 cut(s)	638, 1266
BpmI	CTGGAG	1 cut(s)	683
Bpu14I	TTCGAA	1 cut(s)	1321
BpuEI	CTTGAG	1 cut(s)	476
BpuMI	CCSGG	5 cut(s)	323, 683, 684, 919, 942
BsaAI	YACGTR	3 cut(s)	419, 440, 1605
BsaHI	GRCGYC	3 cut(s)	183, 276, 1035
BsaI	GGTCTC	1 cut(s)	839
BsaJI	CCNNGG	8 cut(s)	321, 682, 1162, 1232, 1279, 1406, 1468, 1481
BsaWI	WCCGGW	2 cut(s)	676, 1365
BsaXI	ACNNNNNCTCC	2 cut(s)	731, 761
Bsc4I	CCNNNNNNNGG	7 cut(s)	683, 972, 1253, 1300, 1473, 1487, 1563
Bse1I	ACTGG	3 cut(s)	382, 469, 1574
Bse3DI	GCAATG	2 cut(s)	12, 458
BseAI	TCCGGA	1 cut(s)	676
BseBI	CCWGG	2 cut(s)	311, 1109
BseDI	CCNNGG	8 cut(s)	321, 682, 1162, 1232, 1279, 1406, 1468, 1481
BseGI	GGATG	7 cut(s)	147, 243, 366, 1158, 1218, 1482, 1647
BseLI	CCNNNNNNNGG	7 cut(s)	683, 972, 1253, 1300, 1473, 1487, 1563
BseMI	GCAATG	2 cut(s)	12, 458
BseNI	ACTGG	3 cut(s)	382, 469, 1574
BseRI	GAGGAG	6 cut(s)	11, 35, 50, 79, 116, 857
BseSI	GKGCMC	3 cut(s)	625, 1157, 1386
BseYI	CCCAGC	1 cut(s)	96
BsgI	GTGCAG	3 cut(s)	785, 1198, 1634
Bsh1236I	CGCG	6 cut(s)	172, 350, 401, 569, 797, 1234
Bsh1285I	CGRYCG	2 cut(s)	345, 405
BshFI	GGCC	7 cut(s)	326, 489, 584, 623, 860, 936, 1405
BshNI	GGYRCC	1 cut(s)	700
BsiEI	CGRYCG	2 cut(s)	345, 405
BsiHKAI	GWGCWC	4 cut(s)	455, 669, 1157, 1386
BsiHKCI	CYCGRG	2 cut(s)	682, 1247
BsiSI	CCGG	7 cut(s)	267, 323, 677, 683, 918, 942, 1366
BsiWI	CGTACG	1 cut(s)	588
BslFI	GGGAC	3 cut(s)	304, 1014, 1540
BslI	CCNNNNNNNGG	7 cut(s)	683, 972, 1253, 1300, 1473, 1487, 1563
BsmAI	GTCTC	4 cut(s)	416, 518, 636, 839
BsmBI	CGTCTC	2 cut(s)	518, 636
BsmFI	GGGAC	3 cut(s)	304, 1014, 1540
BsmI	GAATGC	1 cut(s)	784
BsnI	GGCC	7 cut(s)	326, 489, 584, 623, 860, 936, 1405
Bso31I	GGTCTC	1 cut(s)	839
BsoBI	CYCGRG	2 cut(s)	682, 1247
Bsp119I	TTCGAA	1 cut(s)	1321
Bsp120I	GGGCCC	1 cut(s)	621
Bsp1286I	GDGCHC	6 cut(s)	455, 625, 669, 1008, 1157, 1386
Bsp13I	TCCGGA	1 cut(s)	676
Bsp143I	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
Bsp19I	CCATGG	1 cut(s)	1162
Bsp68I	TCGCGA	1 cut(s)	401
BspANI	GGCC	7 cut(s)	326, 489, 584, 623, 860, 936, 1405
BspEI	TCCGGA	1 cut(s)	676
BspFNI	CGCG	6 cut(s)	172, 350, 401, 569, 797, 1234
BspLI	GGNNCC	5 cut(s)	623, 624, 681, 702, 1030
BspMAI	CTGCAG	1 cut(s)	1445
BspMI	ACCTGC	2 cut(s)	1016, 1369
BspPI	GGATC	3 cut(s)	905, 1053, 1576
BspT104I	TTCGAA	1 cut(s)	1321
BspT107I	GGYRCC	1 cut(s)	700
BspTNI	GGTCTC	1 cut(s)	839
BsrBI	CCGCTC	1 cut(s)	658
BsrDI	GCAATG	2 cut(s)	12, 458
BsrI	ACTGG	3 cut(s)	382, 469, 1574
BssECI	CCNNGG	8 cut(s)	321, 682, 1162, 1232, 1279, 1406, 1468, 1481
BssMI	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
BssNI	GRCGYC	3 cut(s)	183, 276, 1035
BssT1I	CCWWGG	3 cut(s)	1162, 1468, 1481
Bst2UI	CCWGG	2 cut(s)	311, 1109
Bst4CI	ACNGT	7 cut(s)	410, 545, 730, 763, 850, 970, 1140
Bst6I	CTCTTC	3 cut(s)	53, 80, 1341
BstACI	GRCGYC	3 cut(s)	183, 276, 1035
BstBAI	YACGTR	3 cut(s)	419, 440, 1605
BstBI	TTCGAA	1 cut(s)	1321
BstC8I	GCNNGC	8 cut(s)	160, 328, 938, 1000, 1004, 1039, 1302, 1373
BstDEI	CTNAG	1 cut(s)	862
BstDSI	CCRYGG	4 cut(s)	1162, 1232, 1279, 1406
BstF5I	GGATG	7 cut(s)	147, 243, 366, 1158, 1218, 1482, 1647
BstFNI	CGCG	6 cut(s)	172, 350, 401, 569, 797, 1234
BstHHI	GCGC	4 cut(s)	174, 289, 571, 1300
BstKTI	GATC	6 cut(s)	552, 900, 912, 1061, 1335, 1584
BstMAI	GTCTC	4 cut(s)	416, 518, 636, 839
BstMBI	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
BstMCI	CGRYCG	2 cut(s)	345, 405
BstMWI	GCNNNNNNNGC	9 cut(s)	164, 191, 336, 486, 561, 664, 779, 1043, 1381
BstNI	CCWGG	2 cut(s)	311, 1109
BstNSI	RCATGY	1 cut(s)	1598
BstPAI	GACNNNNGTC	1 cut(s)	186
BstSCI	CCNGG	7 cut(s)	309, 321, 681, 682, 917, 940, 1107
BstSFI	CTRYAG	1 cut(s)	1441
BstSLI	GKGCMC	3 cut(s)	625, 1157, 1386
BstSNI	TACGTA	1 cut(s)	1605
BstUI	CGCG	6 cut(s)	172, 350, 401, 569, 797, 1234
BstV2I	GAAGAC	2 cut(s)	638, 1266
BstX2I	RGATCY	2 cut(s)	1058, 1581
BstYI	RGATCY	2 cut(s)	1058, 1581
BsuRI	GGCC	7 cut(s)	326, 489, 584, 623, 860, 936, 1405
BtgI	CCRYGG	4 cut(s)	1162, 1232, 1279, 1406
BtsCI	GGATG	7 cut(s)	147, 243, 366, 1158, 1218, 1482, 1647
BtsI	GCAGTG	1 cut(s)	960
BtsIMutI	CAGTG	2 cut(s)	213, 960
BtuMI	TCGCGA	1 cut(s)	401
BveI	ACCTGC	2 cut(s)	1016, 1369
Cac8I	GCNNGC	8 cut(s)	160, 328, 938, 1000, 1004, 1039, 1302, 1373
CaiI	CAGNNNCTG	1 cut(s)	1295
CfoI	GCGC	4 cut(s)	174, 289, 571, 1300
Cfr13I	GGNCC	6 cut(s)	487, 621, 622, 674, 679, 1028
Cfr42I	CCGCGG	1 cut(s)	1235
Cfr9I	CCCGGG	1 cut(s)	682
CseI	GACGC	3 cut(s)	191, 265, 1043
Csp6I	GTAC	6 cut(s)	385, 472, 589, 838, 1351, 1363
CviAII	CATG	7 cut(s)	34, 514, 577, 994, 1163, 1354, 1595
CviQI	GTAC	6 cut(s)	385, 472, 589, 838, 1351, 1363
DdeI	CTNAG	1 cut(s)	862
DpnI	GATC	6 cut(s)	551, 899, 911, 1060, 1334, 1583
DpnII	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
DrdI	GACNNNNNNGTC	1 cut(s)	1268
DriI	GACNNNNNGTC	1 cut(s)	408
DseDI	GACNNNNNNGTC	1 cut(s)	1268
EaeI	YGGCCR	2 cut(s)	324, 934
Eam1104I	CTCTTC	3 cut(s)	53, 80, 1341
Eam1105I	GACNNNNNGTC	1 cut(s)	408
EarI	CTCTTC	3 cut(s)	53, 80, 1341
EciI	GGCGGA	1 cut(s)	82
Ecl136II	GAGCTC	1 cut(s)	453
Eco105I	TACGTA	1 cut(s)	1605
Eco130I	CCWWGG	3 cut(s)	1162, 1468, 1481
Eco24I	GRGCYC	3 cut(s)	455, 625, 1008
Eco31I	GGTCTC	1 cut(s)	839
Eco47I	GGWCC	3 cut(s)	674, 679, 1028
Eco53kI	GAGCTC	1 cut(s)	453
Eco72I	CACGTG	2 cut(s)	419, 440
Eco88I	CYCGRG	2 cut(s)	682, 1247
EcoICRI	GAGCTC	1 cut(s)	453
EcoO109I	RGGNCCY	2 cut(s)	622, 1028
EcoRI	GAATTC	2 cut(s)	1094, 1531
EcoRII	CCWGG	2 cut(s)	309, 1107
EcoT14I	CCWWGG	3 cut(s)	1162, 1468, 1481
EcoT38I	GRGCYC	3 cut(s)	455, 625, 1008
ErhI	CCWWGG	3 cut(s)	1162, 1468, 1481
Esp3I	CGTCTC	2 cut(s)	518, 636
FaeI	CATG	7 cut(s)	37, 517, 580, 997, 1166, 1357, 1598
FalI	AAGNNNNNCTT	2 cut(s)	1379, 1411
FaqI	GGGAC	3 cut(s)	304, 1014, 1540
FatI	CATG	7 cut(s)	33, 513, 576, 993, 1162, 1353, 1594
FauI	CCCGC	6 cut(s)	633, 790, 824, 995, 1046, 1534
FblI	GTMKAC	1 cut(s)	519
Fnu4HI	GCNGC	5 cut(s)	348, 659, 783, 1235, 1376
FokI	GGATG	6 cut(s)	134, 230, 353, 1145, 1205, 1489
FriOI	GRGCYC	3 cut(s)	455, 625, 1008
Fsp4HI	GCNGC	5 cut(s)	348, 659, 783, 1235, 1376
FspBI	CTAG	1 cut(s)	1575
GlaI	GCGC	4 cut(s)	173, 288, 570, 1299
GluI	GCNGC	5 cut(s)	348, 659, 783, 1235, 1376
GsaI	CCCAGC	1 cut(s)	100
GsuI	CTGGAG	1 cut(s)	683
HaeIII	GGCC	7 cut(s)	326, 489, 584, 623, 860, 936, 1405
HapII	CCGG	7 cut(s)	267, 323, 677, 683, 918, 942, 1366
HgaI	GACGC	3 cut(s)	191, 265, 1043
HhaI	GCGC	4 cut(s)	174, 289, 571, 1300
Hin1I	GRCGYC	3 cut(s)	183, 276, 1035
Hin1II	CATG	7 cut(s)	37, 517, 580, 997, 1166, 1357, 1598
Hin6I	GCGC	4 cut(s)	172, 287, 569, 1298
HinP1I	GCGC	4 cut(s)	172, 287, 569, 1298
HincII	GTYRAC	2 cut(s)	520, 1647
HindII	GTYRAC	2 cut(s)	520, 1647
HinfI	GANTC	2 cut(s)	314, 390
HpaII	CCGG	7 cut(s)	267, 323, 677, 683, 918, 942, 1366
HphI	GGTGA	6 cut(s)	110, 121, 787, 902, 995, 1495
Hpy166II	GTNNAC	5 cut(s)	520, 838, 1155, 1384, 1647
Hpy188I	TCNGA	3 cut(s)	1058, 1063, 1187
Hpy188III	TCNNGA	9 cut(s)	280, 301, 400, 493, 539, 677, 814, 1015, 1031
Hpy8I	GTNNAC	5 cut(s)	520, 838, 1155, 1384, 1647
Hpy99I	CGWCG	7 cut(s)	185, 275, 524, 709, 974, 1037, 1175
HpyAV	CCTTC	1 cut(s)	1405
HpyCH4III	ACNGT	7 cut(s)	410, 545, 730, 763, 850, 970, 1140
HpyCH4IV	ACGT	6 cut(s)	418, 439, 723, 876, 1170, 1604
HpyCH4V	TGCA	9 cut(s)	162, 437, 735, 766, 1155, 1215, 1384, 1443, 1615
HpyF10VI	GCNNNNNNNGC	9 cut(s)	164, 191, 336, 486, 561, 664, 779, 1043, 1381
HpyF3I	CTNAG	1 cut(s)	862
HpySE526I	ACGT	6 cut(s)	418, 439, 723, 876, 1170, 1604
Hsp92I	GRCGYC	3 cut(s)	183, 276, 1035
Hsp92II	CATG	7 cut(s)	37, 517, 580, 997, 1166, 1357, 1598
HspAI	GCGC	4 cut(s)	172, 287, 569, 1298
Kpn2I	TCCGGA	1 cut(s)	676
KspI	CCGCGG	1 cut(s)	1235
Kzo9I	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
LmnI	GCTCC	5 cut(s)	458, 637, 655, 664, 1368
LweI	GCATC	2 cut(s)	870, 1467
MaeI	CTAG	1 cut(s)	1575
MaeII	ACGT	6 cut(s)	418, 439, 723, 876, 1170, 1604
MaeIII	GTNAC	4 cut(s)	211, 724, 890, 1270
MalI	GATC	6 cut(s)	551, 899, 911, 1060, 1334, 1583
MbiI	CCGCTC	1 cut(s)	658
MboI	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
MboII	GAAGA	9 cut(s)	7, 40, 67, 643, 1174, 1195, 1271, 1296, 1358
MflI	RGATCY	2 cut(s)	1058, 1581
MhlI	GDGCHC	6 cut(s)	455, 625, 669, 1008, 1157, 1386
MluCI	AATT	7 cut(s)	248, 426, 751, 945, 1094, 1531, 1618
MlyI	GAGTC	1 cut(s)	308
MmeI	TCCRAC	2 cut(s)	175, 1086
MroI	TCCGGA	1 cut(s)	676
MseI	TTAA	2 cut(s)	429, 1599
MslI	CAYNNNNRTG	2 cut(s)	512, 614
MspA1I	CMGCKG	1 cut(s)	1234
MspI	CCGG	7 cut(s)	267, 323, 677, 683, 918, 942, 1366
MspR9I	CCNGG	7 cut(s)	311, 323, 683, 684, 919, 942, 1109
Mva1269I	GAATGC	1 cut(s)	784
MvaI	CCWGG	2 cut(s)	311, 1109
MvnI	CGCG	6 cut(s)	172, 350, 401, 569, 797, 1234
MwoI	GCNNNNNNNGC	9 cut(s)	164, 191, 336, 486, 561, 664, 779, 1043, 1381
NciI	CCSGG	5 cut(s)	323, 683, 684, 919, 942
NcoI	CCATGG	1 cut(s)	1162
NdeII	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
NlaIII	CATG	7 cut(s)	37, 517, 580, 997, 1166, 1357, 1598
NlaIV	GGNNCC	5 cut(s)	623, 624, 681, 702, 1030
NmeAIII	GCCGAG	1 cut(s)	552
NmuCI	GTSAC	4 cut(s)	211, 724, 890, 1270
NruI	TCGCGA	1 cut(s)	401
NspI	RCATGY	1 cut(s)	1598
NspV	TTCGAA	1 cut(s)	1321
PaqCI	CACCTGC	1 cut(s)	1369
PciI	ACATGT	1 cut(s)	1594
PcsI	WCGNNNNNNNCGW	3 cut(s)	347, 846, 1089
PctI	GAATGC	1 cut(s)	784
PfeI	GAWTC	1 cut(s)	390
Pfl23II	CGTACG	1 cut(s)	588
PflFI	GACNNNGTC	3 cut(s)	275, 529, 1265
PfoI	TCCNGGA	1 cut(s)	309
PkrI	GCNGC	5 cut(s)	349, 660, 784, 1236, 1377
PleI	GAGTC	1 cut(s)	308
PmaCI	CACGTG	2 cut(s)	419, 440
PmlI	CACGTG	2 cut(s)	419, 440
PpsI	GAGTC	1 cut(s)	308
Ppu21I	YACGTR	3 cut(s)	419, 440, 1605
PpuMI	RGGWCCY	1 cut(s)	1028
PscI	ACATGT	1 cut(s)	1594
PshAI	GACNNNNGTC	1 cut(s)	186
PsiI	TTATAA	1 cut(s)	1436
Psp124BI	GAGCTC	1 cut(s)	455
Psp5II	RGGWCCY	1 cut(s)	1028
Psp6I	CCWGG	2 cut(s)	309, 1107
PspCI	CACGTG	2 cut(s)	419, 440
PspFI	CCCAGC	1 cut(s)	96
PspGI	CCWGG	2 cut(s)	309, 1107
PspLI	CGTACG	1 cut(s)	588
PspN4I	GGNNCC	5 cut(s)	623, 624, 681, 702, 1030
PspOMI	GGGCCC	1 cut(s)	621
PspPI	GGNCC	6 cut(s)	487, 621, 622, 674, 679, 1028
PspPPI	RGGWCCY	1 cut(s)	1028
PstI	CTGCAG	1 cut(s)	1445
PstNI	CAGNNNCTG	1 cut(s)	1295
PsuI	RGATCY	2 cut(s)	1058, 1581
PsyI	GACNNNGTC	3 cut(s)	275, 529, 1265
RruI	TCGCGA	1 cut(s)	401
RsaI	GTAC	6 cut(s)	386, 473, 590, 839, 1352, 1364
RsaNI	GTAC	6 cut(s)	385, 472, 589, 838, 1351, 1363
RseI	CAYNNNNRTG	2 cut(s)	512, 614
SacI	GAGCTC	1 cut(s)	455
SacII	CCGCGG	1 cut(s)	1235
SalI	GTCGAC	1 cut(s)	518
SaqAI	TTAA	2 cut(s)	429, 1599
SatI	GCNGC	5 cut(s)	348, 659, 783, 1235, 1376
Sau3AI	GATC	6 cut(s)	549, 897, 909, 1058, 1332, 1581
Sau96I	GGNCC	6 cut(s)	487, 621, 622, 674, 679, 1028
SchI	GAGTC	1 cut(s)	308
ScrFI	CCNGG	7 cut(s)	311, 323, 683, 684, 919, 942, 1109
SduI	GDGCHC	6 cut(s)	455, 625, 669, 1008, 1157, 1386
SfaNI	GCATC	2 cut(s)	870, 1467
SfcI	CTRYAG	1 cut(s)	1441
Sfr303I	CCGCGG	1 cut(s)	1235
SfuI	TTCGAA	1 cut(s)	1321
SgrBI	CCGCGG	1 cut(s)	1235
SinI	GGWCC	3 cut(s)	674, 679, 1028
SmaI	CCCGGG	1 cut(s)	684
SmiMI	CAYNNNNRTG	2 cut(s)	512, 614
SmlI	CTYRAG	1 cut(s)	491
SmoI	CTYRAG	1 cut(s)	491
SnaBI	TACGTA	1 cut(s)	1605
Sse9I	AATT	7 cut(s)	248, 426, 751, 945, 1094, 1531, 1618
SspMI	CTAG	1 cut(s)	1575
SstI	GAGCTC	1 cut(s)	455
StyD4I	CCNGG	7 cut(s)	309, 321, 681, 682, 917, 940, 1107
StyI	CCWWGG	3 cut(s)	1162, 1468, 1481
TaaI	ACNGT	7 cut(s)	410, 545, 730, 763, 850, 970, 1140
TaiI	ACGT	6 cut(s)	421, 442, 726, 879, 1173, 1607
TaqI	TCGA	9 cut(s)	363, 519, 540, 691, 1083, 1173, 1321, 1331, 1421
TaqII	GACCGA	1 cut(s)	419
TasI	AATT	7 cut(s)	248, 426, 751, 945, 1094, 1531, 1618
TatI	WGTACW	1 cut(s)	1350
TauI	GCSGC	5 cut(s)	350, 661, 785, 1237, 1378
TfiI	GAWTC	1 cut(s)	390
Tru1I	TTAA	2 cut(s)	429, 1599
Tru9I	TTAA	2 cut(s)	429, 1599
TscAI	CASTG	2 cut(s)	220, 967
TseFI	GTSAC	4 cut(s)	211, 724, 890, 1270
Tsp45I	GTSAC	4 cut(s)	211, 724, 890, 1270
TspDTI	ATGAA	3 cut(s)	982, 1087, 1168
TspGWI	ACGGA	6 cut(s)	650, 684, 1217, 1296, 1358, 1442
TspMI	CCCGGG	1 cut(s)	682
TspRI	CASTG	2 cut(s)	220, 967
Tth111I	GACNNNGTC	3 cut(s)	275, 529, 1265
VneI	GTGCAC	2 cut(s)	1153, 1382
VpaK11BI	GGWCC	3 cut(s)	674, 679, 1028
XapI	RAATTY	4 cut(s)	945, 1094, 1531, 1618
XceI	RCATGY	1 cut(s)	1598
XmaI	CCCGGG	1 cut(s)	682
XmiI	GTMKAC	1 cut(s)	519
XspI	CTAG	1 cut(s)	1575

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.