Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003889

Physical Location & Seq

Genomic Coordinates Forward (+)

7479308 .. 7481551

Gene Structure 2244 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1078000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGCCGCCCTCCTTATTTAAACCTCCATGGCGATGGCACTGGCAACGCTGTTCATCTCCTTCTTCTCCCGGCAAAACCAAATTCCTCCTTCTCATGGCTTCCCTCTTCCTCTCTCTCCTCTTCCTCACTCTCTACCTCTCTCTATCCTCCGCCTCCCACCACCACCACCAGCCCACATCCCCCCGACCTCAGATCGAACAAGCCTGCAAAGCCACGCGCTTCCCCGACGCGTGTCAAGCCTCCCTCACCAACCTCGCCACTGACATCAGCACCACCCCTGTCCAGACCATCCAATTCGCCGTCCAACTCTCCGGCGACGGGATCGAGACGGCGCAGGGAATGGTGAAAACCATCCTGGACTCGTCCGCTGGCAACCTAAACCGCACGACGGCTGCGAATAACTGCCTGGAAATCCTCGCTAACTCCCAGTACCGAATTTCTCTCGCCACCGACAGTCTCTCGCTCGGTAAAATTAAAAACGCGCGTGCCTCCATGAGCTCCGCGTTGCTCTACCAGTACGGCTGCTGGTCGGGCCTCAAGTACGCCAACGACACCAACATGGTCAACGAGACGATGTCGTTTCTCGACAGTTTGATCGCCAAATCCAGCAACGCGCTCGGCATGATGGCCTCGTACGACAACTTCGGTAACGACACAAAAATATGGGCCCCGCCCAAGACGGAGCGAGACGGGTTCTGGGAGCGGCTGGAGCACGGAGGTCCCGACCCGGGATTTCGGGGCGGGGTGCCGTCGGGTTTAACGACGAACGTGACGGTGTGCAAGGAGAAATCGTGTGATTACAGGACGGTGCAGGAGGCGGTTAATGCCGCACCGGATAACGCCGCGGGAAACGAGAGGTTCGTGATTGGGATAAAGGCCGGGGTGTACGAAGAGACCGTGAGGGTGCCCTTAGAGAAGCGGAACGTGGTGTTTTTAGGTGATGGGATGGGTGAAACGGTCATTACCGGGTCGTTGAATGTGGGCCAGCCCGGAATTTCCACGTACAACACTGCTACCGTCGGGGTTGTTGGTGATGGGTTCATGGCAAGCGGACTCACAATCCAAAACACAGCAGGTCCCGACACCCACCAAGCGGTTGCTTTCCGATCAGACAGCGACCTCTCCGTGATCGAAAACTGCGAATTCATAGGCAATCAAGACACCCTCTACGCACAAGGCAACCGCCAGTTCTACAAATCATGCACCATCCAAGGCAACGTCGATTTCATCTTCGGAAACTCAGCTTCCATCTTCCAAGACTGCACCATCCTCGTCCGTCCACGGCAACTCCAACCCAAGAAAGGCGAAGACAATGTCGTCACAGCCCACGGAAGAACAGACCCTGCGATGACAACGGGTTTCGTGTTTCGAAACTGCTCGATCAACGGCACGGAAGAGTACATGAAGTTGTACCGGAGCAAGCCGGAGGTGCACAAGAACTACTTGGGGAGGCCGTGGAAGGAGTATTCGAGGACGGTTTTTATAAACTGCAGTATGGAAGCTCTTATTACACCCCAAGGGTGGATGCCTTGGAGTGGGGATTTTGCTCTCAACACCCTTTTTTATGGGGAGTTTGGGAATTCTGGACCGGGTTCTGATTTGTCCCAGAGATGGAACTGGACCAGCAAAATCCCATCTGAACATGTTAATGCGTATTCTGTGCAGAATTTTATTCAGGGAGATGAGTGGATGTCAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

567

Amino Acids

62.18

Weight (kDa)

6.23

Isoelectric Point (pI)

38.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	64 - 207	4e-18	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	256 - 561	1.9e-118	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09760 AT5G09760
fragaria_vesca	FvH4_1g24090 FvH4_5g14710
malus_domestica	MD02G1238800.v1.1 MD02G1238900.v1.1 MD07G1077900.v1.1 MD07G1078000.v1.1
prunus_persica	Prupe.2G099900_v2.0.a1 Prupe.2G100200_v2.0.a1 Prupe.2G100500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0120271 RchiOBHm_Chr7g0181681
rosa_laevigata	RLG00000005177 RLG00000018546
rosa_multiflora	Rmu_co8370367.1_g000001 Rmu_co8413969.1_g000001 Rmu_sc0000988.1_g000012 Rmu_sc0004523.1_g000012 Rmu_sc0031357.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123580 Rroxscaffold_3G00271810
rosa_rugosa	Rorug02G0228100 Rorug06G0442700
rosa_samantha	Rh2AG284600 Rh2BG295600 Rh2CG273100 Rh2DG309600 Rh7AG045200 Rh7BG044400 Rh7CG045900 Rh7DG044800
rosa_wichuraiana	Rw2G022820 Rw7G003760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1484
AasI	GACNNNNNNGTC	1 cut(s)	1316
Acc36I	ACCTGC	1 cut(s)	1064
AccB1I	GGYRCC	2 cut(s)	745, 904
AccBSI	CCGCTC	1 cut(s)	703
AccII	CGCG	7 cut(s)	217, 230, 482, 484, 503, 614, 845
AclWI	GGATC	1 cut(s)	329
AcsI	RAATTY	6 cut(s)	80, 435, 993, 1142, 1579, 1666
AfaI	GTAC	8 cut(s)	431, 518, 542, 635, 887, 1004, 1400, 1412
AfiI	CCNNNNNNNGG	9 cut(s)	94, 318, 388, 728, 1093, 1301, 1521, 1535, 1611
AflIII	ACRYGT	2 cut(s)	228, 1642
AgsI	TTSAA	1 cut(s)	976
AjnI	CCWGG	2 cut(s)	354, 405
AluBI	AGCT	3 cut(s)	498, 1244, 1502
AluI	AGCT	3 cut(s)	498, 1244, 1502
Alw21I	GWGCWC	3 cut(s)	500, 714, 1434
Alw26I	GTCTC	5 cut(s)	320, 461, 563, 681, 887
Alw44I	GTGCAC	1 cut(s)	1430
AlwI	GGATC	1 cut(s)	329
AlwNI	CAGNNNCTG	1 cut(s)	1343
Ama87I	CYCGRG	1 cut(s)	727
AoxI	GGCC	6 cut(s)	532, 627, 666, 876, 982, 1451
ApaI	GGGCCC	1 cut(s)	670
ApaLI	GTGCAC	1 cut(s)	1430
ApeKI	GCWGC	2 cut(s)	392, 522
ApoI	RAATTY	6 cut(s)	80, 435, 993, 1142, 1579, 1666
ArsI	GACNNNNNNTTYG	2 cut(s)	1299, 1331
AspLEI	GCGC	4 cut(s)	219, 334, 484, 616
AspS9I	GGNCC	8 cut(s)	532, 666, 667, 719, 982, 1076, 1586, 1620
AsuC2I	CCSGG	7 cut(s)	69, 728, 729, 880, 967, 990, 1590
AsuHPI	GGTGA	5 cut(s)	238, 355, 950, 962, 1043
AsuII	TTCGAA	1 cut(s)	1369
AvaI	CYCGRG	1 cut(s)	727
AvaII	GGWCC	4 cut(s)	719, 1076, 1586, 1620
BaeGI	GKGCMC	3 cut(s)	670, 909, 1434
BanI	GGYRCC	2 cut(s)	745, 904
BanII	GRGCYC	2 cut(s)	500, 670
BarI	GAAGNNNNNNTAC	2 cut(s)	626, 658
BbsI	GAAGAC	1 cut(s)	1314
Bbv12I	GWGCWC	3 cut(s)	500, 714, 1434
BbvI	GCAGC	2 cut(s)	379, 509
BceAI	ACGGC	8 cut(s)	284, 345, 405, 535, 733, 1298, 1402, 1438
BcgI	CGANNNNNNTGC	4 cut(s)	598, 632, 1252, 1286
BciT130I	CCWGG	2 cut(s)	356, 407
BcnI	CCSGG	7 cut(s)	69, 728, 729, 880, 967, 990, 1590
BcoDI	GTCTC	5 cut(s)	320, 461, 563, 681, 887
BfmI	CTRYAG	1 cut(s)	1489
BfuAI	ACCTGC	1 cut(s)	1064
BisI	GCNGC	6 cut(s)	5, 393, 523, 704, 828, 843
BlsI	GCNGC	6 cut(s)	6, 394, 524, 705, 829, 844
Bme1390I	CCNGG	9 cut(s)	69, 356, 407, 728, 729, 880, 967, 990, 1590
Bme18I	GGWCC	4 cut(s)	719, 1076, 1586, 1620
BmeT110I	CYCGRG	1 cut(s)	727
BmgT120I	GGNCC	8 cut(s)	532, 666, 667, 719, 982, 1076, 1586, 1620
BmiI	GGNNCC	6 cut(s)	668, 669, 721, 747, 906, 1078
BmrFI	CCNGG	9 cut(s)	69, 356, 407, 728, 729, 880, 967, 990, 1590
BmrI	ACTGGG	1 cut(s)	421
BmsI	GCATC	1 cut(s)	1515
BmuI	ACTGGG	1 cut(s)	421
BoxI	GACNNNNGTC	1 cut(s)	231
BpiI	GAAGAC	1 cut(s)	1314
BpmI	CTGGAG	1 cut(s)	728
Bpu14I	TTCGAA	1 cut(s)	1369
BpuEI	CTTGAG	1 cut(s)	521
BpuMI	CCSGG	7 cut(s)	69, 728, 729, 880, 967, 990, 1590
BsaAI	YACGTR	1 cut(s)	1002
BsaI	GGTCTC	1 cut(s)	887
BsaWI	WCCGGW	2 cut(s)	832, 1413
BsaXI	ACNNNNNCTCC	4 cut(s)	776, 806, 1272, 1302
Bsc4I	CCNNNNNNNGG	9 cut(s)	94, 318, 388, 728, 1093, 1301, 1521, 1535, 1611
Bse1I	ACTGG	5 cut(s)	44, 427, 514, 1186, 1622
BseBI	CCWGG	2 cut(s)	356, 407
BseGI	GGATG	8 cut(s)	176, 288, 351, 951, 1206, 1266, 1530, 1695
BseLI	CCNNNNNNNGG	9 cut(s)	94, 318, 388, 728, 1093, 1301, 1521, 1535, 1611
BseMII	CTCAG	2 cut(s)	203, 1254
BseNI	ACTGG	5 cut(s)	44, 427, 514, 1186, 1622
BseRI	GAGGAG	1 cut(s)	107
BseSI	GKGCMC	3 cut(s)	670, 909, 1434
BseXI	GCAGC	2 cut(s)	379, 509
BsgI	GTGCAG	3 cut(s)	830, 1246, 1682
Bsh1236I	CGCG	7 cut(s)	217, 230, 482, 484, 503, 614, 845
BshFI	GGCC	6 cut(s)	534, 629, 668, 878, 984, 1453
BshNI	GGYRCC	2 cut(s)	745, 904
BsiHKAI	GWGCWC	3 cut(s)	500, 714, 1434
BsiHKCI	CYCGRG	1 cut(s)	727
BsiWI	CGTACG	1 cut(s)	633
BslFI	GGGAC	3 cut(s)	705, 1062, 1588
BslI	CCNNNNNNNGG	9 cut(s)	94, 318, 388, 728, 1093, 1301, 1521, 1535, 1611
BsmAI	GTCTC	5 cut(s)	320, 461, 563, 681, 887
BsmBI	CGTCTC	3 cut(s)	320, 563, 681
BsmFI	GGGAC	3 cut(s)	705, 1062, 1588
BsnI	GGCC	6 cut(s)	534, 629, 668, 878, 984, 1453
Bso31I	GGTCTC	1 cut(s)	887
BsoBI	CYCGRG	1 cut(s)	727
Bsp119I	TTCGAA	1 cut(s)	1369
Bsp120I	GGGCCC	1 cut(s)	666
Bsp1286I	GDGCHC	5 cut(s)	500, 670, 714, 909, 1434
Bsp143I	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
Bsp19I	CCATGG	1 cut(s)	26
BspANI	GGCC	6 cut(s)	534, 629, 668, 878, 984, 1453
BspCNI	CTCAG	2 cut(s)	202, 1253
BspFNI	CGCG	7 cut(s)	217, 230, 482, 484, 503, 614, 845
BspLI	GGNNCC	6 cut(s)	668, 669, 721, 747, 906, 1078
BspMAI	CTGCAG	1 cut(s)	1493
BspMI	ACCTGC	1 cut(s)	1064
BspPI	GGATC	1 cut(s)	329
BspT104I	TTCGAA	1 cut(s)	1369
BspT107I	GGYRCC	2 cut(s)	745, 904
BspTNI	GGTCTC	1 cut(s)	887
BsrBI	CCGCTC	1 cut(s)	703
BsrI	ACTGG	5 cut(s)	44, 427, 514, 1186, 1622
BssMI	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
BssT1I	CCWWGG	4 cut(s)	26, 1210, 1516, 1529
Bst2UI	CCWGG	2 cut(s)	356, 407
Bst4CI	ACNGT	8 cut(s)	455, 590, 775, 808, 898, 958, 1018, 1477
Bst6I	CTCTTC	4 cut(s)	110, 125, 885, 1389
BstBAI	YACGTR	1 cut(s)	1002
BstBI	TTCGAA	1 cut(s)	1369
BstC8I	GCNNGC	6 cut(s)	205, 370, 486, 986, 1048, 1421
BstDEI	CTNAG	3 cut(s)	189, 910, 1240
BstDSI	CCRYGG	5 cut(s)	26, 843, 1280, 1327, 1454
BstF5I	GGATG	8 cut(s)	176, 288, 351, 951, 1206, 1266, 1530, 1695
BstFNI	CGCG	7 cut(s)	217, 230, 482, 484, 503, 614, 845
BstHHI	GCGC	4 cut(s)	219, 334, 484, 616
BstKTI	GATC	6 cut(s)	195, 324, 597, 1109, 1131, 1383
BstMAI	GTCTC	5 cut(s)	320, 461, 563, 681, 887
BstMBI	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
BstMWI	GCNNNNNNNGC	6 cut(s)	209, 236, 531, 709, 824, 1429
BstNI	CCWGG	2 cut(s)	356, 407
BstNSI	RCATGY	1 cut(s)	1646
BstPAI	GACNNNNGTC	1 cut(s)	231
BstSCI	CCNGG	9 cut(s)	67, 354, 405, 726, 727, 878, 965, 988, 1588
BstSFI	CTRYAG	1 cut(s)	1489
BstSLI	GKGCMC	3 cut(s)	670, 909, 1434
BstUI	CGCG	7 cut(s)	217, 230, 482, 484, 503, 614, 845
BstV1I	GCAGC	2 cut(s)	379, 509
BstV2I	GAAGAC	1 cut(s)	1314
BstXI	CCANNNNNNTGG	1 cut(s)	33
BsuRI	GGCC	6 cut(s)	534, 629, 668, 878, 984, 1453
BtgI	CCRYGG	5 cut(s)	26, 843, 1280, 1327, 1454
BtgZI	GCGATG	2 cut(s)	46, 1361
BtsCI	GGATG	8 cut(s)	176, 288, 351, 951, 1206, 1266, 1530, 1695
BtsI	GCAGTG	1 cut(s)	1008
BtsIMutI	CAGTG	3 cut(s)	37, 258, 1008
BveI	ACCTGC	1 cut(s)	1064
Cac8I	GCNNGC	6 cut(s)	205, 370, 486, 986, 1048, 1421
CaiI	CAGNNNCTG	1 cut(s)	1343
CfoI	GCGC	4 cut(s)	219, 334, 484, 616
Cfr13I	GGNCC	8 cut(s)	532, 666, 667, 719, 982, 1076, 1586, 1620
Cfr42I	CCGCGG	1 cut(s)	846
Cfr9I	CCCGGG	1 cut(s)	727
CseI	GACGC	1 cut(s)	236
Csp6I	GTAC	8 cut(s)	430, 517, 541, 634, 886, 1003, 1399, 1411
CviAII	CATG	9 cut(s)	27, 94, 493, 559, 622, 1042, 1200, 1402, 1643
CviQI	GTAC	8 cut(s)	430, 517, 541, 634, 886, 1003, 1399, 1411
DdeI	CTNAG	3 cut(s)	189, 910, 1240
DpnI	GATC	6 cut(s)	194, 323, 596, 1108, 1130, 1382
DpnII	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
DraI	TTTAAA	1 cut(s)	19
DrdI	GACNNNNNNGTC	1 cut(s)	1316
DseDI	GACNNNNNNGTC	1 cut(s)	1316
Eam1104I	CTCTTC	4 cut(s)	110, 125, 885, 1389
EarI	CTCTTC	4 cut(s)	110, 125, 885, 1389
EciI	GGCGGA	1 cut(s)	139
Ecl136II	GAGCTC	1 cut(s)	498
Eco130I	CCWWGG	4 cut(s)	26, 1210, 1516, 1529
Eco24I	GRGCYC	2 cut(s)	500, 670
Eco31I	GGTCTC	1 cut(s)	887
Eco47I	GGWCC	4 cut(s)	719, 1076, 1586, 1620
Eco53kI	GAGCTC	1 cut(s)	498
Eco88I	CYCGRG	1 cut(s)	727
EcoICRI	GAGCTC	1 cut(s)	498
EcoO109I	RGGNCCY	3 cut(s)	667, 719, 1076
EcoRI	GAATTC	2 cut(s)	1142, 1579
EcoRII	CCWGG	2 cut(s)	354, 405
EcoT14I	CCWWGG	4 cut(s)	26, 1210, 1516, 1529
EcoT38I	GRGCYC	2 cut(s)	500, 670
ErhI	CCWWGG	4 cut(s)	26, 1210, 1516, 1529
Esp3I	CGTCTC	3 cut(s)	320, 563, 681
FaeI	CATG	9 cut(s)	30, 97, 496, 562, 625, 1045, 1203, 1405, 1646
FalI	AAGNNNNNCTT	2 cut(s)	1427, 1459
FaqI	GGGAC	3 cut(s)	705, 1062, 1588
FatI	CATG	9 cut(s)	26, 93, 492, 558, 621, 1041, 1199, 1401, 1642
FauI	CCCGC	3 cut(s)	678, 734, 838
Fnu4HI	GCNGC	6 cut(s)	5, 393, 523, 704, 828, 843
FokI	GGATG	7 cut(s)	163, 275, 338, 958, 1193, 1253, 1537
FriOI	GRGCYC	2 cut(s)	500, 670
Fsp4HI	GCNGC	6 cut(s)	5, 393, 523, 704, 828, 843
GlaI	GCGC	4 cut(s)	218, 333, 483, 615
GluI	GCNGC	6 cut(s)	5, 393, 523, 704, 828, 843
GsuI	CTGGAG	1 cut(s)	728
HaeIII	GGCC	6 cut(s)	534, 629, 668, 878, 984, 1453
HgaI	GACGC	1 cut(s)	236
HhaI	GCGC	4 cut(s)	219, 334, 484, 616
Hin1II	CATG	9 cut(s)	30, 97, 496, 562, 625, 1045, 1203, 1405, 1646
Hin6I	GCGC	4 cut(s)	217, 332, 482, 614
HinP1I	GCGC	4 cut(s)	217, 332, 482, 614
HincII	GTYRAC	2 cut(s)	565, 1695
HindII	GTYRAC	2 cut(s)	565, 1695
HinfI	GANTC	2 cut(s)	359, 1053
HphI	GGTGA	5 cut(s)	238, 355, 950, 962, 1043
Hpy166II	GTNNAC	5 cut(s)	565, 886, 1280, 1432, 1695
Hpy188I	TCNGA	6 cut(s)	192, 1106, 1111, 1235, 1597, 1639
Hpy188III	TCNNGA	8 cut(s)	283, 325, 584, 722, 862, 1079, 1157, 1584
Hpy8I	GTNNAC	5 cut(s)	565, 886, 1280, 1432, 1695
Hpy99I	CGWCG	7 cut(s)	230, 320, 391, 754, 766, 1022, 1223
HpyAV	CCTTC	3 cut(s)	69, 98, 1453
HpyCH4III	ACNGT	8 cut(s)	455, 590, 775, 808, 898, 958, 1018, 1477
HpyCH4IV	ACGT	4 cut(s)	768, 924, 1001, 1218
HpyCH4V	TGCA	8 cut(s)	207, 780, 811, 1203, 1263, 1432, 1491, 1663
HpyF10VI	GCNNNNNNNGC	6 cut(s)	209, 236, 531, 709, 824, 1429
HpyF3I	CTNAG	3 cut(s)	189, 910, 1240
HpySE526I	ACGT	4 cut(s)	768, 924, 1001, 1218
Hsp92II	CATG	9 cut(s)	30, 97, 496, 562, 625, 1045, 1203, 1405, 1646
HspAI	GCGC	4 cut(s)	217, 332, 482, 614
KspI	CCGCGG	1 cut(s)	846
Kzo9I	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
LmnI	GCTCC	5 cut(s)	503, 682, 700, 709, 1416
Lsp1109I	GCAGC	2 cut(s)	379, 509
LweI	GCATC	1 cut(s)	1515
MaeII	ACGT	4 cut(s)	768, 924, 1001, 1218
MaeIII	GTNAC	3 cut(s)	647, 769, 1318
MalI	GATC	6 cut(s)	194, 323, 596, 1108, 1130, 1382
MbiI	CCGCTC	1 cut(s)	703
MboI	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
MboII	GAAGA	9 cut(s)	54, 97, 112, 902, 1222, 1243, 1319, 1344, 1406
MhlI	GDGCHC	5 cut(s)	500, 670, 714, 909, 1434
MluCI	AATT	8 cut(s)	80, 293, 435, 471, 993, 1142, 1579, 1666
MluI	ACGCGT	1 cut(s)	228
MlyI	GAGTC	2 cut(s)	353, 1047
MmeI	TCCRAC	2 cut(s)	328, 1315
MseI	TTAA	5 cut(s)	18, 474, 758, 822, 1647
MslI	CAYNNNNRTG	3 cut(s)	31, 557, 1647
MspA1I	CMGCKG	2 cut(s)	368, 845
MspR9I	CCNGG	9 cut(s)	69, 356, 407, 728, 729, 880, 967, 990, 1590
MvaI	CCWGG	2 cut(s)	356, 407
MvnI	CGCG	7 cut(s)	217, 230, 482, 484, 503, 614, 845
MwoI	GCNNNNNNNGC	6 cut(s)	209, 236, 531, 709, 824, 1429
NciI	CCSGG	7 cut(s)	69, 728, 729, 880, 967, 990, 1590
NcoI	CCATGG	1 cut(s)	26
NdeII	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
NlaIII	CATG	9 cut(s)	30, 97, 496, 562, 625, 1045, 1203, 1405, 1646
NlaIV	GGNNCC	6 cut(s)	668, 669, 721, 747, 906, 1078
NmeAIII	GCCGAG	1 cut(s)	597
NmuCI	GTSAC	2 cut(s)	769, 1318
NspI	RCATGY	1 cut(s)	1646
NspV	TTCGAA	1 cut(s)	1369
PciI	ACATGT	1 cut(s)	1642
PcsI	WCGNNNNNNNCGW	6 cut(s)	392, 720, 758, 858, 894, 1137
Pfl23II	CGTACG	1 cut(s)	633
PflFI	GACNNNGTC	2 cut(s)	574, 1313
PfoI	TCCNGGA	1 cut(s)	354
PkrI	GCNGC	6 cut(s)	6, 394, 524, 705, 829, 844
PleI	GAGTC	2 cut(s)	353, 1047
PpsI	GAGTC	2 cut(s)	353, 1047
Ppu21I	YACGTR	1 cut(s)	1002
PpuMI	RGGWCCY	2 cut(s)	719, 1076
PscI	ACATGT	1 cut(s)	1642
PshAI	GACNNNNGTC	1 cut(s)	231
PsiI	TTATAA	1 cut(s)	1484
Psp124BI	GAGCTC	1 cut(s)	500
Psp5II	RGGWCCY	2 cut(s)	719, 1076
Psp6I	CCWGG	2 cut(s)	354, 405
PspGI	CCWGG	2 cut(s)	354, 405
PspLI	CGTACG	1 cut(s)	633
PspN4I	GGNNCC	6 cut(s)	668, 669, 721, 747, 906, 1078
PspOMI	GGGCCC	1 cut(s)	666
PspPI	GGNCC	8 cut(s)	532, 666, 667, 719, 982, 1076, 1586, 1620
PspPPI	RGGWCCY	2 cut(s)	719, 1076
PstI	CTGCAG	1 cut(s)	1493
PstNI	CAGNNNCTG	1 cut(s)	1343
PsyI	GACNNNGTC	2 cut(s)	574, 1313
RsaI	GTAC	8 cut(s)	431, 518, 542, 635, 887, 1004, 1400, 1412
RsaNI	GTAC	8 cut(s)	430, 517, 541, 634, 886, 1003, 1399, 1411
RseI	CAYNNNNRTG	3 cut(s)	31, 557, 1647
SacI	GAGCTC	1 cut(s)	500
SacII	CCGCGG	1 cut(s)	846
SaqAI	TTAA	5 cut(s)	18, 474, 758, 822, 1647
SatI	GCNGC	6 cut(s)	5, 393, 523, 704, 828, 843
Sau3AI	GATC	6 cut(s)	192, 321, 594, 1106, 1128, 1380
Sau96I	GGNCC	8 cut(s)	532, 666, 667, 719, 982, 1076, 1586, 1620
SchI	GAGTC	2 cut(s)	353, 1047
ScrFI	CCNGG	9 cut(s)	69, 356, 407, 728, 729, 880, 967, 990, 1590
SduI	GDGCHC	5 cut(s)	500, 670, 714, 909, 1434
SfaNI	GCATC	1 cut(s)	1515
SfcI	CTRYAG	1 cut(s)	1489
Sfr303I	CCGCGG	1 cut(s)	846
SfuI	TTCGAA	1 cut(s)	1369
SgrBI	CCGCGG	1 cut(s)	846
SinI	GGWCC	4 cut(s)	719, 1076, 1586, 1620
SmaI	CCCGGG	1 cut(s)	729
SmiMI	CAYNNNNRTG	3 cut(s)	31, 557, 1647
SmlI	CTYRAG	1 cut(s)	536
SmoI	CTYRAG	1 cut(s)	536
Sse9I	AATT	8 cut(s)	80, 293, 435, 471, 993, 1142, 1579, 1666
SstI	GAGCTC	1 cut(s)	500
StyD4I	CCNGG	9 cut(s)	67, 354, 405, 726, 727, 878, 965, 988, 1588
StyI	CCWWGG	4 cut(s)	26, 1210, 1516, 1529
TaaI	ACNGT	8 cut(s)	455, 590, 775, 808, 898, 958, 1018, 1477
TaiI	ACGT	4 cut(s)	771, 927, 1004, 1221
TaqI	TCGA	8 cut(s)	195, 324, 585, 1131, 1221, 1369, 1379, 1469
TasI	AATT	8 cut(s)	80, 293, 435, 471, 993, 1142, 1579, 1666
TatI	WGTACW	1 cut(s)	1398
TauI	GCSGC	4 cut(s)	7, 706, 830, 845
Tru1I	TTAA	5 cut(s)	18, 474, 758, 822, 1647
Tru9I	TTAA	5 cut(s)	18, 474, 758, 822, 1647
TscAI	CASTG	3 cut(s)	44, 265, 1015
TseFI	GTSAC	2 cut(s)	769, 1318
TseI	GCWGC	2 cut(s)	392, 522
Tsp45I	GTSAC	2 cut(s)	769, 1318
TspDTI	ATGAA	5 cut(s)	42, 1030, 1135, 1216, 1418
TspGWI	ACGGA	6 cut(s)	695, 729, 1114, 1265, 1344, 1406
TspMI	CCCGGG	1 cut(s)	727
TspRI	CASTG	3 cut(s)	44, 265, 1015
Tth111I	GACNNNGTC	2 cut(s)	574, 1313
VneI	GTGCAC	1 cut(s)	1430
VpaK11BI	GGWCC	4 cut(s)	719, 1076, 1586, 1620
XapI	RAATTY	6 cut(s)	80, 435, 993, 1142, 1579, 1666
XceI	RCATGY	1 cut(s)	1646
XmaI	CCCGGG	1 cut(s)	727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.