FvH4_2g08950

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003460
Physical Location & Seq
Forward (+)
7851601 .. 7852101
501 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08950.t1

Sequence Viewer

Length: 501 bp
ATGAAGCTAGCAGGGGTGAGACCCCAAACCCTAGAGATCGAACCAGGAACTGTAATGAACTTCTGGGTTCCTTCTGAAACCAACTCGAAAAAGTCGAAGCCAGCCGTGGTCTTTCTTCACGGGTTTGCAGTGGACGGCATCATAACATGGCAATTTCAAGTCCTATCTCTCGCCAGAGACTACGCCGTTTACGTACCCGATCTTATCTTCTTCGGTGGATCCATCACGGATAAACCCGACCGGTCGCCGGAGTTCCAAGCCGAGTGTGTGGCGAAGGGTTTGAGGAAGCTTGGGGTGAAGAGGTGCACCCTTGTGGGGTTGAGCTACGGAGGCATGGTGGGGTTTAAGATGGCGGAGCTGTACCCTGATATGGTTGAGGCCGTTGTCTTGACTTGCTCCGTTATGGCTTTGACCAAGTCCGTTAGCGACGCCGGACTTGGGAGGATTGGGTTCTCACGGTGGACGGGTTACTTGCTTCCCGACTCTGTCAAAGGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

167

Amino Acids

18.07

Weight (kDa)

8.77

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 34 - 135 1.7e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 36 - 140 8.9e-10 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 353
AclWI GGATC 2 cut(s) 213, 226
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 2 cut(s) 195, 362
AfiI CCNNNNNNNGG 4 cut(s) 247, 315, 370, 438
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 1 cut(s) 158
AjnI CCWGG 1 cut(s) 43
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 4 cut(s) 7, 289, 324, 358
AluI AGCT 4 cut(s) 7, 289, 324, 358
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 2 cut(s) 13, 171
Alw44I GTGCAC 1 cut(s) 304
AlwI GGATC 2 cut(s) 213, 226
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 1 cut(s) 378
ApaLI GTGCAC 1 cut(s) 304
AsiGI ACCGGT 1 cut(s) 240
AsuHPI GGTGA 2 cut(s) 28, 307
AsuNHI GCTAGC 1 cut(s) 7
BaeGI GKGCMC 1 cut(s) 308
BamHI GGATCC 1 cut(s) 218
Bbv12I GWGCWC 1 cut(s) 308
BccI CCATC 2 cut(s) 230, 343
BceAI ACGGC 4 cut(s) 89, 151, 170, 365
BciT130I CCWGG 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 13, 171
BfaI CTAG 2 cut(s) 8, 32
Bme1390I CCNGG 1 cut(s) 45
BmiI GGNNCC 2 cut(s) 69, 220
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 1 cut(s) 147
BmtI GCTAGC 1 cut(s) 11
BsaAI YACGTR 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 105
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 315, 370, 438
Bse118I RCCGGY 1 cut(s) 240
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 105
BseLI CCNNNNNNNGG 4 cut(s) 247, 315, 370, 438
BseSI GKGCMC 1 cut(s) 308
Bsh1285I CGRYCG 2 cut(s) 241, 245
BshFI GGCC 1 cut(s) 380
BshTI ACCGGT 1 cut(s) 240
BsiEI CGRYCG 2 cut(s) 241, 245
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 3 cut(s) 241, 248, 432
BslI CCNNNNNNNGG 4 cut(s) 247, 315, 370, 438
BsmAI GTCTC 2 cut(s) 13, 171
BsnI GGCC 1 cut(s) 380
Bso31I GGTCTC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 3 cut(s) 36, 199, 218
BspACI CCGC 1 cut(s) 353
BspANI GGCC 1 cut(s) 380
BspLI GGNNCC 2 cut(s) 69, 220
BspOI GCTAGC 1 cut(s) 11
BspPI GGATC 2 cut(s) 213, 226
BspTNI GGTCTC 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 3 cut(s) 36, 199, 218
BssNI GRCGYC 1 cut(s) 429
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 52, 459
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 429
BstBAI YACGTR 1 cut(s) 193
BstC8I GCNNGC 2 cut(s) 9, 102
BstDSI CCRYGG 1 cut(s) 105
BstKTI GATC 3 cut(s) 39, 202, 221
BstMAI GTCTC 2 cut(s) 13, 171
BstMBI GATC 3 cut(s) 36, 199, 218
BstMCI CGRYCG 2 cut(s) 241, 245
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 43
BstSLI GKGCMC 1 cut(s) 308
BstSNI TACGTA 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BsuRI GGCC 1 cut(s) 380
BtgI CCRYGG 1 cut(s) 105
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 9, 102
CaiI CAGNNNCTG 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 240
CseI GACGC 1 cut(s) 437
Csp6I GTAC 2 cut(s) 194, 361
CspAI ACCGGT 1 cut(s) 240
CviAII CATG 2 cut(s) 147, 334
CviJI RGCY 9 cut(s) 7, 100, 104, 260, 289, 324, 358, 380, 407
CviKI_1 RGCY 9 cut(s) 7, 100, 104, 260, 289, 324, 358, 380, 407
CviQI GTAC 2 cut(s) 194, 361
DpnI GATC 3 cut(s) 38, 201, 220
DpnII GATC 3 cut(s) 36, 199, 218
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 1 cut(s) 368
Eco105I TACGTA 1 cut(s) 193
Eco31I GGTCTC 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 2 cut(s) 150, 337
FaiI YATR 5 cut(s) 143, 148, 335, 371, 404
FatI CATG 2 cut(s) 146, 333
FspBI CTAG 2 cut(s) 8, 32
HaeIII GGCC 1 cut(s) 380
HapII CCGG 3 cut(s) 241, 248, 432
HgaI GACGC 1 cut(s) 437
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 2 cut(s) 150, 337
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 2 cut(s) 482, 495
HpaII CCGG 3 cut(s) 241, 248, 432
HphI GGTGA 2 cut(s) 28, 307
Hpy166II GTNNAC 4 cut(s) 133, 190, 306, 462
Hpy188I TCNGA 2 cut(s) 76, 500
Hpy188III TCNNGA 2 cut(s) 388, 479
Hpy8I GTNNAC 4 cut(s) 133, 190, 306, 462
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 2 cut(s) 81, 268
HpyCH4III ACNGT 2 cut(s) 52, 459
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 128, 306
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 2 cut(s) 150, 337
Kzo9I GATC 3 cut(s) 36, 199, 218
LmnI GCTCC 2 cut(s) 355, 401
LpnPI CCDG 9 cut(s) 30, 49, 57, 114, 187, 254, 261, 378, 445
LweI GCATC 1 cut(s) 147
MaeI CTAG 2 cut(s) 8, 32
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 467
MalI GATC 3 cut(s) 38, 201, 220
MboI GATC 3 cut(s) 36, 199, 218
MboII GAAGA 4 cut(s) 107, 199, 202, 310
MflI RGATCY 1 cut(s) 218
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 1 cut(s) 152
MlyI GAGTC 1 cut(s) 476
MnlI CCTC 5 cut(s) 276, 294, 323, 370, 435
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 311
MspI CCGG 3 cut(s) 241, 248, 432
MspR9I CCNGG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 330
NdeII GATC 3 cut(s) 36, 199, 218
NheI GCTAGC 1 cut(s) 7
NlaIII CATG 2 cut(s) 150, 337
NlaIV GGNNCC 2 cut(s) 69, 220
NmeAIII GCCGAG 1 cut(s) 286
OliI CACNNNNGTG 1 cut(s) 311
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 189
PflFI GACNNNGTC 2 cut(s) 415, 485
PinAI ACCGGT 1 cut(s) 240
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
Ppu21I YACGTR 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 69, 220
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 218
PsyI GACNNNGTC 2 cut(s) 415, 485
RsaI GTAC 2 cut(s) 195, 362
RsaNI GTAC 2 cut(s) 194, 361
RseI CAYNNNNRTG 1 cut(s) 311
SaqAI TTAA 1 cut(s) 345
Sau3AI GATC 3 cut(s) 36, 199, 218
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 1 cut(s) 308
SetI ASST 6 cut(s) 9, 195, 291, 305, 326, 360
SfaNI GCATC 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 311
SnaBI TACGTA 1 cut(s) 193
Sse9I AATT 1 cut(s) 152
SsiI CCGC 1 cut(s) 353
SspMI CTAG 2 cut(s) 8, 32
StyD4I CCNGG 1 cut(s) 43
TaaI ACNGT 2 cut(s) 52, 459
TaiI ACGT 1 cut(s) 195
TaqI TCGA 3 cut(s) 39, 86, 95
TasI AATT 1 cut(s) 152
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 1 cut(s) 135
TspDTI ATGAA 2 cut(s) 17, 71
TspGWI ACGGA 4 cut(s) 242, 342, 388, 409
TspRI CASTG 1 cut(s) 135
Tth111I GACNNNGTC 2 cut(s) 415, 485
VneI GTGCAC 1 cut(s) 304
XspI CTAG 2 cut(s) 8, 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.