Rroxscaffold_175G00431960

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000175
OG0003460
Physical Location & Seq
Forward (+)
75264 .. 76294
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_175G00431960.1

Sequence Viewer

Length: 735 bp
ATGGGAAACATTTTTGCAATATACAAGCCCATCTTGCATGGACTAATGAAGCTGGCCGGGGTGAGACCCCAAACCCTAGAGATCGAACCAGGAACTGTAATGAACTTCTGGGTTCCTACTGAAACCAACTCGAAGAAATCGAAGCCAGCCGTGGTCTTTCTTCACGGTTTCGCAGTGGACGGCATCATTACATGGCAATTCCAAGTCCTATCTCTCGCCAGATACTACGCCGTTTACGTACCCGATCTCATCTTCTTCGGTGGATCCATTACGGATAAGCCCGACCGGTCGCCGGAGTTTCAAGCCGAGTGCGTAGCCAAGGGTTTGAGGAAGCTTGGGGTGGAGAGGTGCACCCTTGTGGGGTTGAGCTATGGAGGCATGGTGGGGTTTAAGATGGCGGAGCTGTACCCCGATTTGGTTGAGGCCTTTGTTGTGACTTGCTCCGTTATGGCTTTGAACAAGTCCGTTAGCGACGCCGGACTTGGGAGAATTGGGTTCTCACGGTGGTCGGATTACTTGCTTGCTGACTCGGTCAAAGGAGTGAGAAACATTTTTGAAATTGCTGCCTACAAGTTTCCTCATCTTCCTGATTGGGTATACAGACACTATTTGGAAGTAATGTTTGACAACCGAAGGGAGAGGGAAGAACTTCTAGAGGCTTTGGTCGTCAGTGACGAGGATTTCACCGTGGCTAATTATCCACAGTTCCACCATGCATGCATGATGCATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

244

Amino Acids

27.59

Weight (kDa)

6.17

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 49 - 175 3.6e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 51 - 178 8.4e-10 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 597
AciI CCGC 1 cut(s) 398
AclWI GGATC 2 cut(s) 258, 271
AcoI YGGCCR 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 2 cut(s) 240, 407
AfiI CCNNNNNNNGG 4 cut(s) 292, 360, 415, 483
AgeI ACCGGT 1 cut(s) 285
AgsI TTSAA 3 cut(s) 302, 457, 557
AjnI CCWGG 1 cut(s) 88
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 4 cut(s) 52, 334, 369, 403
AluI AGCT 4 cut(s) 52, 334, 369, 403
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 58
Alw44I GTGCAC 1 cut(s) 349
AlwI GGATC 2 cut(s) 258, 271
AlwNI CAGNNNCTG 1 cut(s) 95
AoxI GGCC 2 cut(s) 54, 423
ApaLI GTGCAC 1 cut(s) 349
ApeKI GCWGC 1 cut(s) 563
AsiGI ACCGGT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 648
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 73, 676
BaeGI GKGCMC 1 cut(s) 353
BamHI GGATCC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 550
BccI CCATC 2 cut(s) 38, 388
BceAI ACGGC 3 cut(s) 134, 196, 215
BciT130I CCWGG 1 cut(s) 90
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 2 cut(s) 77, 653
BisI GCNGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 565
Bme1390I CCNGG 2 cut(s) 58, 90
BmiI GGNNCC 2 cut(s) 114, 265
BmrFI CCNGG 2 cut(s) 58, 90
BmsI GCATC 2 cut(s) 192, 714
BpuMI CCSGG 1 cut(s) 58
BsaAI YACGTR 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 58
BsaJI CCNNGG 4 cut(s) 57, 150, 318, 687
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 4 cut(s) 292, 360, 415, 483
Bse118I RCCGGY 1 cut(s) 285
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 4 cut(s) 57, 150, 318, 687
BseLI CCNNNNNNNGG 4 cut(s) 292, 360, 415, 483
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 1 cut(s) 550
Bsh1285I CGRYCG 2 cut(s) 286, 290
BshFI GGCC 2 cut(s) 56, 425
BshTI ACCGGT 1 cut(s) 285
BsiEI CGRYCG 2 cut(s) 286, 290
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 4 cut(s) 57, 286, 293, 477
BslI CCNNNNNNNGG 4 cut(s) 292, 360, 415, 483
BsmAI GTCTC 1 cut(s) 58
BsnI GGCC 2 cut(s) 56, 425
Bso31I GGTCTC 1 cut(s) 58
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 81, 244, 263
BspACI CCGC 1 cut(s) 398
BspANI GGCC 2 cut(s) 56, 425
BspLI GGNNCC 2 cut(s) 114, 265
BspPI GGATC 2 cut(s) 258, 271
BspTNI GGTCTC 1 cut(s) 58
BsrFI RCCGGY 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 285
BssECI CCNNGG 4 cut(s) 57, 150, 318, 687
BssMI GATC 3 cut(s) 81, 244, 263
BssNAI GTATAC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 318
Bst1107I GTATAC 1 cut(s) 598
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 5 cut(s) 97, 167, 504, 688, 705
BstACI GRCGYC 1 cut(s) 474
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 54, 147, 522, 718
BstDSI CCRYGG 2 cut(s) 150, 687
BstKTI GATC 3 cut(s) 84, 247, 266
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 3 cut(s) 81, 244, 263
BstMCI CGRYCG 2 cut(s) 286, 290
BstMWI GCNNNNNNNGC 2 cut(s) 34, 375
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 2 cut(s) 56, 88
BstSLI GKGCMC 1 cut(s) 353
BstSNI TACGTA 1 cut(s) 238
BstV1I GCAGC 1 cut(s) 550
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BstZ17I GTATAC 1 cut(s) 598
BsuRI GGCC 2 cut(s) 56, 425
BtgI CCRYGG 2 cut(s) 150, 687
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 180, 676
Cac8I GCNNGC 4 cut(s) 54, 147, 522, 718
CaiI CAGNNNCTG 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 285
CseI GACGC 1 cut(s) 482
Csp6I GTAC 2 cut(s) 239, 406
CspAI ACCGGT 1 cut(s) 285
CviAII CATG 6 cut(s) 38, 192, 379, 713, 717, 721
CviQI GTAC 2 cut(s) 239, 406
DpnI GATC 3 cut(s) 83, 246, 265
DpnII GATC 3 cut(s) 81, 244, 263
EaeI YGGCCR 1 cut(s) 54
EciI GGCGGA 1 cut(s) 413
Eco105I TACGTA 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 318
Eco147I AGGCCT 1 cut(s) 425
Eco31I GGTCTC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 318
EcoT22I ATGCAT 3 cut(s) 718, 722, 729
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 6 cut(s) 41, 195, 382, 716, 720, 724
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FatI CATG 6 cut(s) 37, 191, 378, 712, 716, 720
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 1 cut(s) 564
Fsp4HI GCNGC 1 cut(s) 564
FspBI CTAG 2 cut(s) 77, 653
GluI GCNGC 1 cut(s) 564
HaeIII GGCC 2 cut(s) 56, 425
HapII CCGG 4 cut(s) 57, 286, 293, 477
HgaI GACGC 1 cut(s) 482
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 6 cut(s) 41, 195, 382, 716, 720, 724
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 1 cut(s) 527
HpaII CCGG 4 cut(s) 57, 286, 293, 477
HphI GGTGA 2 cut(s) 73, 676
Hpy166II GTNNAC 4 cut(s) 178, 235, 351, 598
Hpy188I TCNGA 1 cut(s) 511
Hpy188III TCNNGA 2 cut(s) 587, 653
Hpy8I GTNNAC 4 cut(s) 178, 235, 351, 598
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 1 cut(s) 627
HpyCH4III ACNGT 5 cut(s) 97, 167, 504, 688, 705
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 6 cut(s) 17, 37, 351, 716, 720, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 375
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 6 cut(s) 41, 195, 382, 716, 720, 724
Kzo9I GATC 3 cut(s) 81, 244, 263
LmnI GCTCC 2 cut(s) 400, 446
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 2 cut(s) 192, 714
MaeI CTAG 2 cut(s) 77, 653
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 433, 671
MalI GATC 3 cut(s) 83, 246, 265
MboI GATC 3 cut(s) 81, 244, 263
MboII GAAGA 6 cut(s) 145, 152, 244, 247, 575, 656
MflI RGATCY 1 cut(s) 263
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 4 cut(s) 197, 489, 558, 694
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 8 cut(s) 321, 339, 368, 415, 588, 633, 649, 670
Mph1103I ATGCAT 3 cut(s) 718, 722, 729
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 356
MspI CCGG 4 cut(s) 57, 286, 293, 477
MspR9I CCNGG 2 cut(s) 58, 90
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 34, 375
NciI CCSGG 1 cut(s) 58
NdeII GATC 3 cut(s) 81, 244, 263
NlaIII CATG 6 cut(s) 41, 195, 382, 716, 720, 724
NlaIV GGNNCC 2 cut(s) 114, 265
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 433, 671
NsiI ATGCAT 3 cut(s) 718, 722, 729
NspI RCATGY 1 cut(s) 720
OliI CACNNNNGTG 1 cut(s) 356
PaeI GCATGC 1 cut(s) 720
PceI AGGCCT 1 cut(s) 425
PcsI WCGNNNNNNNCGW 3 cut(s) 137, 234, 672
PdmI GAANNNNTTC 1 cut(s) 648
PflFI GACNNNGTC 1 cut(s) 530
PinAI ACCGGT 1 cut(s) 285
PkrI GCNGC 1 cut(s) 565
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 2 cut(s) 114, 265
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 530
RsaI GTAC 2 cut(s) 240, 407
RsaNI GTAC 2 cut(s) 239, 406
RseI CAYNNNNRTG 1 cut(s) 356
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 1 cut(s) 564
Sau3AI GATC 3 cut(s) 81, 244, 263
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 2 cut(s) 58, 90
SduI GDGCHC 1 cut(s) 353
SetI ASST 6 cut(s) 54, 240, 336, 350, 371, 405
SfaNI GCATC 2 cut(s) 192, 714
SmiMI CAYNNNNRTG 1 cut(s) 356
SnaBI TACGTA 1 cut(s) 238
SphI GCATGC 1 cut(s) 720
Sse9I AATT 4 cut(s) 197, 489, 558, 694
SseBI AGGCCT 1 cut(s) 425
SsiI CCGC 1 cut(s) 398
SspMI CTAG 2 cut(s) 77, 653
StuI AGGCCT 1 cut(s) 425
StyD4I CCNGG 2 cut(s) 56, 88
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 5 cut(s) 97, 167, 504, 688, 705
TaiI ACGT 1 cut(s) 240
TaqI TCGA 3 cut(s) 84, 131, 140
TaqII GACCGA 1 cut(s) 520
TasI AATT 4 cut(s) 197, 489, 558, 694
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 2 cut(s) 180, 676
TseFI GTSAC 2 cut(s) 433, 671
TseI GCWGC 1 cut(s) 563
Tsp45I GTSAC 2 cut(s) 433, 671
TspDTI ATGAA 2 cut(s) 62, 116
TspGWI ACGGA 3 cut(s) 287, 433, 454
TspRI CASTG 2 cut(s) 180, 676
Tth111I GACNNNGTC 1 cut(s) 530
VneI GTGCAC 1 cut(s) 349
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 2 cut(s) 77, 653
Zsp2I ATGCAT 3 cut(s) 718, 722, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.