Rmu_sc0005111.1_g000001

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005111.1
OG0003460
Physical Location & Seq
Forward (+)
462 .. 1162
701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005111.1_g000001.1.cds

Sequence Viewer

Length: 612 bp
atgaacttctgggttcctactgaaaccaactcgaagaaatcgaagccagccgtggtctttcttcacggtttcgcagtggacggcatcataacatggcaattccaagtcctatctctcgctagatactacgccgtatacgtacccgatctcatcttcttcggtgaatccattacggataagcccgaccggtcgcctgagtttcaagccgagtgcgtagccaagggtttgaggaagcttggggtggagaggtgcacccttgtggggttgagctatggaggcatggtggggtttaagatggcggagctgtaccccgatttggttgaggcctttgttgtgacttccaccgttatggctttgaacaagtccgttagcgacgccggacttgggaggattgggttctcacggtggtcggattacttgcttcctgactcggccaaaggagtgagaaacctttttgaaattgctgcctacaagtttcctcatcttcctgattgggtctacagacactatttggaagcaatgtttgacaaccaaagggagagggaagaacttctaaaggctttggttatcagtgacgaggatttcactgtggctaattatccacaggtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

203

Amino Acids

23.01

Weight (kDa)

5.44

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 135, 498
AciI CCGC 1 cut(s) 299
AcoI YGGCCR 1 cut(s) 432
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 2 cut(s) 141, 308
AfiI CCNNNNNNNGG 3 cut(s) 261, 316, 384
AgeI ACCGGT 1 cut(s) 186
AgsI TTSAA 3 cut(s) 203, 358, 458
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 3 cut(s) 235, 270, 304
AluI AGCT 3 cut(s) 235, 270, 304
Alw21I GWGCWC 1 cut(s) 254
Alw44I GTGCAC 1 cut(s) 250
AoxI GGCC 2 cut(s) 324, 432
ApaLI GTGCAC 1 cut(s) 250
ApeKI GCWGC 1 cut(s) 464
AsiGI ACCGGT 1 cut(s) 186
Asp700I GAANNNNTTC 1 cut(s) 549
AsuHPI GGTGA 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 451
BccI CCATC 1 cut(s) 289
BceAI ACGGC 3 cut(s) 35, 97, 116
BfaI CTAG 1 cut(s) 120
BfmI CTRYAG 1 cut(s) 499
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
BmiI GGNNCC 1 cut(s) 15
BmsI GCATC 1 cut(s) 93
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 51, 219
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 316, 384
Bse118I RCCGGY 1 cut(s) 186
Bse3DI GCAATG 1 cut(s) 525
BseDI CCNNGG 2 cut(s) 51, 219
BseLI CCNNNNNNNGG 3 cut(s) 261, 316, 384
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 1 cut(s) 186
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 1 cut(s) 451
Bsh1285I CGRYCG 2 cut(s) 187, 191
BshFI GGCC 2 cut(s) 326, 434
BshTI ACCGGT 1 cut(s) 186
BsiEI CGRYCG 2 cut(s) 187, 191
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 2 cut(s) 187, 378
BslI CCNNNNNNNGG 3 cut(s) 261, 316, 384
BsnI GGCC 2 cut(s) 326, 434
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 1 cut(s) 145
BspACI CCGC 1 cut(s) 299
BspANI GGCC 2 cut(s) 326, 434
BspCNI CTCAG 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 2 cut(s) 51, 219
BssMI GATC 1 cut(s) 145
BssNAI GTATAC 1 cut(s) 136
BssNI GRCGYC 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 136
Bst4CI ACNGT 4 cut(s) 68, 346, 405, 589
BstACI GRCGYC 1 cut(s) 375
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 51
BstKTI GATC 1 cut(s) 148
BstMBI GATC 1 cut(s) 145
BstMCI CGRYCG 2 cut(s) 187, 191
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstSFI CTRYAG 1 cut(s) 499
BstSLI GKGCMC 1 cut(s) 254
BstSNI TACGTA 1 cut(s) 139
BstV1I GCAGC 1 cut(s) 451
BstXI CCANNNNNNTGG 1 cut(s) 349
BstZ17I GTATAC 1 cut(s) 136
BsuRI GGCC 2 cut(s) 326, 434
BtgI CCRYGG 1 cut(s) 51
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 3 cut(s) 81, 577, 585
Cac8I GCNNGC 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 186
CseI GACGC 1 cut(s) 383
Csp6I GTAC 2 cut(s) 140, 307
CspAI ACCGGT 1 cut(s) 186
CviAII CATG 2 cut(s) 93, 280
CviQI GTAC 2 cut(s) 140, 307
DdeI CTNAG 1 cut(s) 195
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
EaeI YGGCCR 1 cut(s) 432
EciI GGCGGA 1 cut(s) 314
Eco105I TACGTA 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 219
Eco147I AGGCCT 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 2 cut(s) 96, 283
FaiI YATR 6 cut(s) 89, 94, 136, 273, 281, 350
FatI CATG 2 cut(s) 92, 279
FblI GTMKAC 2 cut(s) 135, 498
Fnu4HI GCNGC 1 cut(s) 465
Fsp4HI GCNGC 1 cut(s) 465
FspBI CTAG 1 cut(s) 120
GluI GCNGC 1 cut(s) 465
HaeIII GGCC 2 cut(s) 326, 434
HapII CCGG 2 cut(s) 187, 378
HgaI GACGC 1 cut(s) 383
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 2 cut(s) 96, 283
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 2 cut(s) 164, 428
HpaII CCGG 2 cut(s) 187, 378
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 4 cut(s) 79, 136, 252, 499
Hpy188I TCNGA 1 cut(s) 412
Hpy188III TCNNGA 2 cut(s) 425, 488
Hpy8I GTNNAC 4 cut(s) 79, 136, 252, 499
Hpy99I CGWCG 1 cut(s) 377
HpyCH4III ACNGT 4 cut(s) 68, 346, 405, 589
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 1 cut(s) 252
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 2 cut(s) 96, 283
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 1 cut(s) 301
LpnPI CCDG 7 cut(s) 60, 200, 207, 391, 438, 501, 590
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 120
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 334, 572
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 6 cut(s) 46, 53, 145, 148, 476, 557
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 3 cut(s) 98, 459, 595
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 8 cut(s) 222, 240, 269, 316, 381, 489, 534, 571
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 2 cut(s) 257, 347
MspI CCGG 2 cut(s) 187, 378
MwoI GCNNNNNNNGC 1 cut(s) 276
NdeII GATC 1 cut(s) 145
NlaIII CATG 2 cut(s) 96, 283
NlaIV GGNNCC 1 cut(s) 15
NmeAIII GCCGAG 2 cut(s) 232, 410
NmuCI GTSAC 2 cut(s) 334, 572
OliI CACNNNNGTG 1 cut(s) 257
PceI AGGCCT 1 cut(s) 326
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 135
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 186
PkrI GCNGC 1 cut(s) 466
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 15
RsaI GTAC 2 cut(s) 141, 308
RsaNI GTAC 2 cut(s) 140, 307
RseI CAYNNNNRTG 2 cut(s) 257, 347
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 1 cut(s) 145
SchI GAGTC 1 cut(s) 422
SduI GDGCHC 1 cut(s) 254
SetI ASST 7 cut(s) 141, 237, 251, 272, 306, 453, 609
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 1 cut(s) 499
SmiMI CAYNNNNRTG 2 cut(s) 257, 347
SnaBI TACGTA 1 cut(s) 139
Sse9I AATT 3 cut(s) 98, 459, 595
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 1 cut(s) 299
SspMI CTAG 1 cut(s) 120
StuI AGGCCT 1 cut(s) 326
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 68, 346, 405, 589
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 32, 41
TasI AATT 3 cut(s) 98, 459, 595
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 3 cut(s) 81, 577, 592
TseFI GTSAC 2 cut(s) 334, 572
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 2 cut(s) 334, 572
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 188, 355
TspRI CASTG 3 cut(s) 81, 577, 592
VneI GTGCAC 1 cut(s) 250
XmiI GTMKAC 2 cut(s) 135, 498
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.