Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003460

Physical Location & Seq

Genomic Coordinates Reverse (-)

11795645 .. 11797317

Gene Structure 1673 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1065200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 912 bp

ATGGGGAACATTTTTCCAGTCTACAAGTTTATTCTGCATGGGGTTATGAAGCTAGTAGGGATGAGACCCCAAACAGTGGAAATCGAGCCAGGAACTATCATGAACTTCTGGGTGCCCAATAAAATTACAGAAAAGCAGAAATCCAAGCCCGCCGTGGTATTCATCCATGGGTTTGCAATGGATGGTATCACCACATGGCAATTTCAAGTGCTATCTCTTGTTAAAGACTACGCGGTTTATGTTCCGGACCTTCTCTTCTTTGGAGGCTCCACTACGGATAAACCGGATAGGTCACTGGAGTTTCAAGCGGAGTGTGTGGCAAAGGGTTTGAGGATACTTGGGGTGGAGAGGTGCACCGTGGTGGGGCTGAGCTATGGAGGCATGGTTGGGTTTAAGATGGCTGAGTTGTACCCTAATTTGGTTGAGTCTATGGTGGTGACTTGTTCAGTTATGGCTTTAACCAGGTCAATTACTAATGCATCTCTTGATAGACTTGGGTTCAAATGCTGGTCAGATTACTTGCTTCCCGACTCAGTCAAAGGTGTGAGAAACCTTTTTGAAATTGCTGCCTATAAATTTCCGCATCTCCCAGACTGGATTTACAAACACTATTTAGAGGTTATGTTTGAACACAGAAAAGAGAGTGAGGAACTTTTGGATGCATTGATTGTGGATGACAAGGACTTTACAGCGGCTCATTACCCACAGCGTGTGCATCTTTTGTGGGGAGAGAATGACAAGATTTTCAACATGGAAACTGCCAATAATCTCAAACTCCAACTTGGAGACAACGCAACTCTACAGTCCATAGAGAAGACGGGCCACCTTGTTCAAGTGGAAGGACTATTTGTATACAACAAGGAACTCAAGAAATTTCTCTCTTCCCTGTTAGAAGAAAATGTGCACGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1

Protein Analysis

304

Amino Acids

34.53

Weight (kDa)

5.88

Isoelectric Point (pI)

36.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	50 - 149	7.3e-17	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	52 - 279	1.8e-11	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003460)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08950 FvH4_4g26240 FvH4_4g26240
malus_domestica	MD05G1065200.v1.1 MD10G1074900.v1.1
prunus_persica	Prupe.8G095900_v2.0.a1 Prupe.8G095900_v2.0.a1 Prupe.8G095900_v2.0.a1 Prupe.8G095900_v2.0.a1 Prupe.8G095900_v2.0.a1 Prupe.8G095900_v2.0.a1 Prupe.8G096000_v2.0.a1 Prupe.8G096000_v2.0.a1
pyrus_communis	pycom05g05650
rosa_chinensis	RchiOBHm_Chr6g0264071 RchiOBHm_Chr6g0264591
rosa_laevigata	RLG00000014242 RLG00000025226 RLG00000030776
rosa_multiflora	Rmu_sc0000578.1_g000025 Rmu_sc0005111.1_g000001 Rmu_sc0006339.1_g000003
rosa_roxburghii	Rroxscaffold_175G00431960 Rroxscaffold_7G00203310
rosa_samantha	Rh6AG129100 Rh6AG133500 Rh6BG124700 Rh6BG131100 Rh6CG123400 Rh6CG129200 Rh6CG129600 Rh6DG110400 Rh6DG116400 Rh6DG116600
rosa_wichuraiana	Rw6G011150 Rw6G011580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	112
AccB7I	CCANNNNNTGG	1 cut(s)	76
AccI	GTMKAC	2 cut(s)	21, 852
AccII	CGCG	1 cut(s)	233
AccIII	TCCGGA	1 cut(s)	244
AciI	CCGC	5 cut(s)	150, 233, 308, 581, 692
AcsI	RAATTY	2 cut(s)	575, 872
AdeI	CACNNNGTG	1 cut(s)	710
AfaI	GTAC	1 cut(s)	410
AfiI	CCNNNNNNNGG	3 cut(s)	76, 363, 418
AgsI	TTSAA	7 cut(s)	206, 305, 502, 560, 629, 748, 833
AjnI	CCWGG	2 cut(s)	88, 461
AleI	CACNNNNGTG	1 cut(s)	359
AluBI	AGCT	2 cut(s)	52, 372
AluI	AGCT	2 cut(s)	52, 372
Alw21I	GWGCWC	2 cut(s)	356, 906
Alw26I	GTCTC	2 cut(s)	58, 780
Alw44I	GTGCAC	2 cut(s)	352, 902
Aor13HI	TCCGGA	1 cut(s)	244
AoxI	GGCC	1 cut(s)	820
ApaLI	GTGCAC	2 cut(s)	352, 902
ApeKI	GCWGC	1 cut(s)	566
ApoI	RAATTY	2 cut(s)	575, 872
AspS9I	GGNCC	2 cut(s)	247, 820
AsuHPI	GGTGA	2 cut(s)	181, 448
AvaII	GGWCC	1 cut(s)	247
BaeGI	GKGCMC	3 cut(s)	117, 356, 906
BanI	GGYRCC	1 cut(s)	112
BbsI	GAAGAC	1 cut(s)	821
Bbv12I	GWGCWC	2 cut(s)	356, 906
BbvI	GCAGC	1 cut(s)	553
BccI	CCATC	2 cut(s)	176, 391
BceAI	ACGGC	1 cut(s)	137
BciT130I	CCWGG	2 cut(s)	90, 463
BciVI	GTATCC	1 cut(s)	327
BcoDI	GTCTC	2 cut(s)	58, 780
BfaI	CTAG	1 cut(s)	53
BfmI	CTRYAG	1 cut(s)	800
BfuI	GTATCC	1 cut(s)	327
BisI	GCNGC	2 cut(s)	567, 693
BlpI	GCTNAGC	1 cut(s)	368
BlsI	GCNGC	2 cut(s)	568, 694
Bme1390I	CCNGG	2 cut(s)	90, 463
Bme18I	GGWCC	1 cut(s)	247
BmgT120I	GGNCC	2 cut(s)	247, 820
BmiI	GGNNCC	2 cut(s)	114, 268
BmrFI	CCNGG	2 cut(s)	90, 463
BmsI	GCATC	4 cut(s)	488, 592, 649, 724
BpiI	GAAGAC	1 cut(s)	821
BpmI	CTGGAG	1 cut(s)	317
Bpu1102I	GCTNAGC	1 cut(s)	368
BpuEI	CTTGAG	1 cut(s)	851
BsaI	GGTCTC	1 cut(s)	58
BsaJI	CCNNGG	3 cut(s)	153, 166, 357
BsaWI	WCCGGW	2 cut(s)	244, 283
BsaXI	ACNNNNNCTCC	2 cut(s)	369, 399
Bsc4I	CCNNNNNNNGG	3 cut(s)	76, 363, 418
Bse1I	ACTGG	3 cut(s)	17, 300, 599
Bse3DI	GCAATG	1 cut(s)	183
BseAI	TCCGGA	1 cut(s)	244
BseBI	CCWGG	2 cut(s)	90, 463
BseDI	CCNNGG	3 cut(s)	153, 166, 357
BseGI	GGATG	5 cut(s)	66, 162, 187, 664, 679
BseLI	CCNNNNNNNGG	3 cut(s)	76, 363, 418
BseMI	GCAATG	1 cut(s)	183
BseMII	CTCAG	3 cut(s)	359, 393, 546
BseNI	ACTGG	3 cut(s)	17, 300, 599
BseSI	GKGCMC	3 cut(s)	117, 356, 906
BseXI	GCAGC	1 cut(s)	553
Bsh1236I	CGCG	1 cut(s)	233
BshFI	GGCC	1 cut(s)	822
BshNI	GGYRCC	1 cut(s)	112
BsiHKAI	GWGCWC	2 cut(s)	356, 906
BsiSI	CCGG	2 cut(s)	245, 284
BslI	CCNNNNNNNGG	3 cut(s)	76, 363, 418
BsmAI	GTCTC	2 cut(s)	58, 780
BsnI	GGCC	1 cut(s)	822
Bso31I	GGTCTC	1 cut(s)	58
Bsp1286I	GDGCHC	3 cut(s)	117, 356, 906
Bsp13I	TCCGGA	1 cut(s)	244
Bsp1720I	GCTNAGC	1 cut(s)	368
Bsp19I	CCATGG	1 cut(s)	166
BspACI	CCGC	5 cut(s)	150, 233, 308, 581, 692
BspANI	GGCC	1 cut(s)	822
BspCNI	CTCAG	3 cut(s)	360, 394, 545
BspEI	TCCGGA	1 cut(s)	244
BspFNI	CGCG	1 cut(s)	233
BspHI	TCATGA	1 cut(s)	99
BspLI	GGNNCC	2 cut(s)	114, 268
BspT107I	GGYRCC	1 cut(s)	112
BspTNI	GGTCTC	1 cut(s)	58
BsrDI	GCAATG	1 cut(s)	183
BsrI	ACTGG	3 cut(s)	17, 300, 599
BssECI	CCNNGG	3 cut(s)	153, 166, 357
BssNAI	GTATAC	1 cut(s)	853
BssT1I	CCWWGG	1 cut(s)	166
Bst1107I	GTATAC	1 cut(s)	853
Bst2UI	CCWGG	2 cut(s)	90, 463
Bst4CI	ACNGT	3 cut(s)	76, 358, 804
Bst6I	CTCTTC	2 cut(s)	260, 886
BstC8I	GCNNGC	1 cut(s)	150
BstDEI	CTNAG	3 cut(s)	368, 402, 532
BstDSI	CCRYGG	3 cut(s)	153, 166, 357
BstF5I	GGATG	5 cut(s)	66, 162, 187, 664, 679
BstFNI	CGCG	1 cut(s)	233
BstMAI	GTCTC	2 cut(s)	58, 780
BstMWI	GCNNNNNNNGC	1 cut(s)	378
BstNI	CCWGG	2 cut(s)	90, 463
BstSCI	CCNGG	2 cut(s)	88, 461
BstSFI	CTRYAG	1 cut(s)	800
BstSLI	GKGCMC	3 cut(s)	117, 356, 906
BstUI	CGCG	1 cut(s)	233
BstV1I	GCAGC	1 cut(s)	553
BstV2I	GAAGAC	1 cut(s)	821
BstZ17I	GTATAC	1 cut(s)	853
BsuI	GTATCC	1 cut(s)	327
BsuRI	GGCC	1 cut(s)	822
BtgI	CCRYGG	3 cut(s)	153, 166, 357
BtsCI	GGATG	5 cut(s)	66, 162, 187, 664, 679
BtsIMutI	CAGTG	2 cut(s)	81, 293
Cac8I	GCNNGC	1 cut(s)	150
CciI	TCATGA	1 cut(s)	99
Cfr13I	GGNCC	2 cut(s)	247, 820
CsiI	ACCWGGT	1 cut(s)	461
Csp6I	GTAC	1 cut(s)	409
CviAII	CATG	6 cut(s)	38, 100, 167, 195, 382, 751
CviQI	GTAC	1 cut(s)	409
DdeI	CTNAG	3 cut(s)	368, 402, 532
DraIII	CACNNNGTG	1 cut(s)	710
Eam1104I	CTCTTC	2 cut(s)	260, 886
EarI	CTCTTC	2 cut(s)	260, 886
Eco130I	CCWWGG	1 cut(s)	166
Eco31I	GGTCTC	1 cut(s)	58
Eco47I	GGWCC	1 cut(s)	247
EcoRII	CCWGG	2 cut(s)	88, 461
EcoT14I	CCWWGG	1 cut(s)	166
EcoT22I	ATGCAT	2 cut(s)	481, 664
ErhI	CCWWGG	1 cut(s)	166
FaeI	CATG	6 cut(s)	41, 103, 170, 198, 385, 754
FatI	CATG	6 cut(s)	37, 99, 166, 194, 381, 750
FauI	CCCGC	1 cut(s)	157
FblI	GTMKAC	2 cut(s)	21, 852
Fnu4HI	GCNGC	2 cut(s)	567, 693
FokI	GGATG	5 cut(s)	73, 149, 194, 671, 686
Fsp4HI	GCNGC	2 cut(s)	567, 693
FspBI	CTAG	1 cut(s)	53
GluI	GCNGC	2 cut(s)	567, 693
GsuI	CTGGAG	1 cut(s)	317
HaeIII	GGCC	1 cut(s)	822
HapII	CCGG	2 cut(s)	245, 284
Hin1II	CATG	6 cut(s)	41, 103, 170, 198, 385, 754
HinfI	GANTC	2 cut(s)	425, 530
HpaII	CCGG	2 cut(s)	245, 284
HphI	GGTGA	2 cut(s)	181, 448
Hpy166II	GTNNAC	4 cut(s)	22, 354, 853, 904
Hpy188I	TCNGA	1 cut(s)	514
Hpy188III	TCNNGA	5 cut(s)	100, 245, 485, 527, 868
Hpy8I	GTNNAC	4 cut(s)	22, 354, 853, 904
HpyAV	CCTTC	2 cut(s)	260, 833
HpyCH4III	ACNGT	3 cut(s)	76, 358, 804
HpyCH4V	TGCA	7 cut(s)	37, 176, 354, 479, 662, 715, 904
HpyF10VI	GCNNNNNNNGC	1 cut(s)	378
HpyF3I	CTNAG	3 cut(s)	368, 402, 532
Hsp92II	CATG	6 cut(s)	41, 103, 170, 198, 385, 754
Kpn2I	TCCGGA	1 cut(s)	244
LmnI	GCTCC	1 cut(s)	272
Lsp1109I	GCAGC	1 cut(s)	553
LweI	GCATC	4 cut(s)	488, 592, 649, 724
MabI	ACCWGGT	1 cut(s)	461
MaeI	CTAG	1 cut(s)	53
MaeIII	GTNAC	2 cut(s)	291, 436
MboII	GAAGA	4 cut(s)	247, 826, 873, 905
MhlI	GDGCHC	3 cut(s)	117, 356, 906
MluCI	AATT	7 cut(s)	123, 200, 415, 468, 561, 575, 872
MlyI	GAGTC	2 cut(s)	434, 524
MmeI	TCCRAC	1 cut(s)	802
MnlI	CCTC	6 cut(s)	257, 324, 342, 371, 610, 640
Mph1103I	ATGCAT	2 cut(s)	481, 664
MroI	TCCGGA	1 cut(s)	244
MseI	TTAA	3 cut(s)	222, 393, 458
MslI	CAYNNNNRTG	1 cut(s)	359
MspA1I	CMGCKG	1 cut(s)	692
MspI	CCGG	2 cut(s)	245, 284
MspR9I	CCNGG	2 cut(s)	90, 463
MvaI	CCWGG	2 cut(s)	90, 463
MvnI	CGCG	1 cut(s)	233
MwoI	GCNNNNNNNGC	1 cut(s)	378
NcoI	CCATGG	1 cut(s)	166
NlaIII	CATG	6 cut(s)	41, 103, 170, 198, 385, 754
NlaIV	GGNNCC	2 cut(s)	114, 268
NmuCI	GTSAC	2 cut(s)	291, 436
NsiI	ATGCAT	2 cut(s)	481, 664
OliI	CACNNNNGTG	1 cut(s)	359
PagI	TCATGA	1 cut(s)	99
PflFI	GACNNNGTC	1 cut(s)	533
PflMI	CCANNNNNTGG	1 cut(s)	76
PkrI	GCNGC	2 cut(s)	568, 694
PleI	GAGTC	2 cut(s)	433, 524
PpsI	GAGTC	2 cut(s)	433, 524
Psp6I	CCWGG	2 cut(s)	88, 461
PspGI	CCWGG	2 cut(s)	88, 461
PspN4I	GGNNCC	2 cut(s)	114, 268
PspPI	GGNCC	2 cut(s)	247, 820
PsyI	GACNNNGTC	1 cut(s)	533
RsaI	GTAC	1 cut(s)	410
RsaNI	GTAC	1 cut(s)	409
RseI	CAYNNNNRTG	1 cut(s)	359
SaqAI	TTAA	3 cut(s)	222, 393, 458
SatI	GCNGC	2 cut(s)	567, 693
Sau96I	GGNCC	2 cut(s)	247, 820
SchI	GAGTC	2 cut(s)	434, 524
ScrFI	CCNGG	2 cut(s)	90, 463
SduI	GDGCHC	3 cut(s)	117, 356, 906
SexAI	ACCWGGT	1 cut(s)	461
SfaNI	GCATC	4 cut(s)	488, 592, 649, 724
SfcI	CTRYAG	1 cut(s)	800
SinI	GGWCC	1 cut(s)	247
SmiMI	CAYNNNNRTG	1 cut(s)	359
SmlI	CTYRAG	1 cut(s)	866
SmoI	CTYRAG	1 cut(s)	866
Sse9I	AATT	7 cut(s)	123, 200, 415, 468, 561, 575, 872
SsiI	CCGC	5 cut(s)	150, 233, 308, 581, 692
SspMI	CTAG	1 cut(s)	53
StyD4I	CCNGG	2 cut(s)	88, 461
StyI	CCWWGG	1 cut(s)	166
TaaI	ACNGT	3 cut(s)	76, 358, 804
TaqI	TCGA	1 cut(s)	84
TasI	AATT	7 cut(s)	123, 200, 415, 468, 561, 575, 872
TauI	GCSGC	1 cut(s)	695
Tru1I	TTAA	3 cut(s)	222, 393, 458
Tru9I	TTAA	3 cut(s)	222, 393, 458
TscAI	CASTG	2 cut(s)	81, 300
TseFI	GTSAC	2 cut(s)	291, 436
TseI	GCWGC	1 cut(s)	566
Tsp45I	GTSAC	2 cut(s)	291, 436
TspDTI	ATGAA	3 cut(s)	62, 116, 151
TspGWI	ACGGA	1 cut(s)	290
TspRI	CASTG	2 cut(s)	81, 300
Tth111I	GACNNNGTC	1 cut(s)	533
Van91I	CCANNNNNTGG	1 cut(s)	76
VneI	GTGCAC	2 cut(s)	352, 902
VpaK11BI	GGWCC	1 cut(s)	247
XapI	RAATTY	2 cut(s)	575, 872
XcmI	CCANNNNNNNNNTGG	1 cut(s)	151
XmiI	GTMKAC	2 cut(s)	21, 852
XspI	CTAG	1 cut(s)	53
Zsp2I	ATGCAT	2 cut(s)	481, 664

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.