RLG00000030776

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003460
Physical Location & Seq
Reverse (-)
67316853 .. 67317212
360 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030776

Sequence Viewer

Length: 360 bp
ATGGCCGATCGCTGTAGACAATTGCAGGGTGACAATGATCCAAGGCCTCATAATATTGAGAAACCTCCTAGAGTCCCGCGGCCGGTGGTCCACCAGGTGTACAGAGTGAATGAGAAGGCTGCTTCAAAAATTCAGACTACAGAGGTTCAACCCTTAGCTGAGATAGTTGAAACTAATGCTATGGTTCCATCCTGTGCAGAAGCAATGCAGGTACAAGATGGTCAAGATTCTTTACAGGTTTCCATGAGGAATATAGAACCTACTGACAATGGGAGAGCTAGAAGTCCAAGGAGTACAGTTTTGCTTCCGAGGCCGGGAGATCTTACTACCAATGAAAACCAGTTTGCCATTTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

13.25

Weight (kDa)

6.09

Isoelectric Point (pI)

60.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
AccI GTMKAC 1 cut(s) 16
AccII CGCG 1 cut(s) 79
AciI CCGC 2 cut(s) 77, 79
AclWI GGATC 1 cut(s) 32
AcoI YGGCCR 2 cut(s) 3, 80
AcsI RAATTY 1 cut(s) 129
AdeI CACNNNGTG 1 cut(s) 97
AfaI GTAC 3 cut(s) 101, 213, 295
AfiI CCNNNNNNNGG 2 cut(s) 82, 314
AgsI TTSAA 3 cut(s) 126, 149, 170
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 2 cut(s) 158, 278
AluI AGCT 2 cut(s) 158, 278
AlwI GGATC 1 cut(s) 32
AoxI GGCC 5 cut(s) 3, 44, 80, 311, 354
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 41
AvaII GGWCC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 106
BccI CCATC 2 cut(s) 196, 212
BciT130I CCWGG 1 cut(s) 95
BcnI CCSGG 1 cut(s) 315
BfaI CTAG 2 cut(s) 69, 279
BfmI CTRYAG 2 cut(s) 13, 138
BfuAI ACCTGC 1 cut(s) 199
BglI GCCNNNNNGGC 1 cut(s) 353
BglII AGATCT 1 cut(s) 319
BisI GCNGC 2 cut(s) 80, 120
BlsI GCNGC 2 cut(s) 81, 121
Bme1390I CCNGG 2 cut(s) 95, 315
Bme18I GGWCC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 2 cut(s) 95, 315
Bpu10I CCTNAGC 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 315
BsaJI CCNNGG 4 cut(s) 41, 77, 287, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 314
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 4 cut(s) 41, 77, 287, 308
BseGI GGATG 1 cut(s) 188
BseLI CCNNNNNNNGG 2 cut(s) 82, 314
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 340
BseX3I CGGCCG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 79
Bsh1285I CGRYCG 2 cut(s) 10, 83
BshFI GGCC 5 cut(s) 5, 46, 82, 313, 356
BsiEI CGRYCG 2 cut(s) 10, 83
BsiSI CCGG 2 cut(s) 83, 314
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 2 cut(s) 82, 314
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 5 cut(s) 5, 46, 82, 313, 356
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 3 cut(s) 7, 37, 319
BspACI CCGC 2 cut(s) 77, 79
BspANI GGCC 5 cut(s) 5, 46, 82, 313, 356
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 186
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 4 cut(s) 41, 77, 287, 308
BssMI GATC 3 cut(s) 7, 37, 319
BssT1I CCWWGG 2 cut(s) 41, 287
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 298
BstAUI TGTACA 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 154, 159
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 1 cut(s) 188
BstFNI CGCG 1 cut(s) 79
BstKTI GATC 3 cut(s) 10, 40, 322
BstMBI GATC 3 cut(s) 7, 37, 319
BstMCI CGRYCG 2 cut(s) 10, 83
BstMWI GCNNNNNNNGC 2 cut(s) 310, 353
BstNI CCWGG 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 93, 313
BstSFI CTRYAG 2 cut(s) 13, 138
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 1 cut(s) 106
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BstZI CGGCCG 1 cut(s) 80
BsuRI GGCC 5 cut(s) 5, 46, 82, 313, 356
BtgI CCRYGG 1 cut(s) 77
BtsCI GGATG 1 cut(s) 188
BveI ACCTGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 88
Cfr42I CCGCGG 1 cut(s) 80
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 3 cut(s) 100, 212, 294
CviAII CATG 1 cut(s) 244
CviJI RGCY 8 cut(s) 5, 46, 82, 119, 158, 278, 313, 356
CviKI_1 RGCY 8 cut(s) 5, 46, 82, 119, 158, 278, 313, 356
CviQI GTAC 3 cut(s) 100, 212, 294
DdeI CTNAG 2 cut(s) 154, 159
DpnI GATC 3 cut(s) 9, 39, 321
DpnII GATC 3 cut(s) 7, 37, 319
DraIII CACNNNGTG 1 cut(s) 97
EaeI YGGCCR 2 cut(s) 3, 80
EagI CGGCCG 1 cut(s) 80
EclXI CGGCCG 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 41, 287
Eco147I AGGCCT 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 88
Eco52I CGGCCG 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 2 cut(s) 41, 287
ErhI CCWWGG 2 cut(s) 41, 287
FaeI CATG 1 cut(s) 247
FaiI YATR 4 cut(s) 51, 182, 245, 254
FaqI GGGAC 1 cut(s) 59
FatI CATG 1 cut(s) 243
FauI CCCGC 1 cut(s) 84
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 80, 120
FokI GGATG 1 cut(s) 175
Fsp4HI GCNGC 2 cut(s) 80, 120
FspBI CTAG 2 cut(s) 69, 279
GluI GCNGC 2 cut(s) 80, 120
HaeIII GGCC 5 cut(s) 5, 46, 82, 313, 356
HapII CCGG 2 cut(s) 83, 314
Hin1II CATG 1 cut(s) 247
HinfI GANTC 2 cut(s) 72, 227
HpaII CCGG 2 cut(s) 83, 314
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 3 cut(s) 17, 91, 100
Hpy188I TCNGA 2 cut(s) 135, 309
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 3 cut(s) 17, 91, 100
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4V TGCA 3 cut(s) 25, 197, 208
HpyF10VI GCNNNNNNNGC 2 cut(s) 310, 353
HpyF3I CTNAG 2 cut(s) 154, 159
Hsp92II CATG 1 cut(s) 247
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 3 cut(s) 7, 37, 319
LpnPI CCDG 9 cut(s) 11, 80, 96, 107, 194, 205, 221, 327, 353
Lsp1109I GCAGC 1 cut(s) 106
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 2 cut(s) 69, 279
MaeIII GTNAC 1 cut(s) 29
MalI GATC 3 cut(s) 9, 39, 321
MboI GATC 3 cut(s) 7, 37, 319
MfeI CAATTG 1 cut(s) 20
MflI RGATCY 1 cut(s) 319
MluCI AATT 2 cut(s) 20, 129
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 5 cut(s) 57, 75, 136, 240, 303
MspA1I CMGCKG 1 cut(s) 79
MspI CCGG 2 cut(s) 83, 314
MspR9I CCNGG 2 cut(s) 95, 315
MunI CAATTG 1 cut(s) 20
MvaI CCWGG 1 cut(s) 95
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 310, 353
NciI CCSGG 1 cut(s) 315
NdeII GATC 3 cut(s) 7, 37, 319
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 29
PceI AGGCCT 1 cut(s) 46
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 81, 121
Ple19I CGATCG 1 cut(s) 10
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 1 cut(s) 88
PsuI RGATCY 1 cut(s) 319
PvuI CGATCG 1 cut(s) 10
RsaI GTAC 3 cut(s) 101, 213, 295
RsaNI GTAC 3 cut(s) 100, 212, 294
SacII CCGCGG 1 cut(s) 80
SatI GCNGC 2 cut(s) 80, 120
Sau3AI GATC 3 cut(s) 7, 37, 319
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 95, 315
SetI ASST 8 cut(s) 67, 99, 147, 160, 213, 240, 262, 280
SexAI ACCWGGT 1 cut(s) 93
SfcI CTRYAG 2 cut(s) 13, 138
Sfr303I CCGCGG 1 cut(s) 80
SgrBI CCGCGG 1 cut(s) 80
SinI GGWCC 1 cut(s) 88
Sse9I AATT 2 cut(s) 20, 129
SseBI AGGCCT 1 cut(s) 46
SsiI CCGC 2 cut(s) 77, 79
SspI AATATT 1 cut(s) 55
SspMI CTAG 2 cut(s) 69, 279
StuI AGGCCT 1 cut(s) 46
StyD4I CCNGG 2 cut(s) 93, 313
StyI CCWWGG 2 cut(s) 41, 287
TaaI ACNGT 1 cut(s) 298
TasI AATT 2 cut(s) 20, 129
TatI WGTACW 2 cut(s) 99, 293
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 1 cut(s) 227
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 348
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 1 cut(s) 129
XmiI GTMKAC 1 cut(s) 16
XspI CTAG 2 cut(s) 69, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.