FvH4_2g27580

Cyclin_C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001536
Physical Location & Seq
Forward (+)
21848387 .. 21853395
5009 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27580.t2

Sequence Viewer

Length: 1521 bp
ATGTCGACCCGGAACCGCCGTGCACCGCCGTCCTCGGGTAACAGGGCACCGATTCCGCAGAATCCGAACAAGAAGGCCATGGCGGCGAAGCCTCAAGTGGCGAAGAAACGAACGGCGCTGTGTGACGTCACCAACCAAAGAAATGGGTCTCAAACTGGTCCATGGAGCTCTGCTTCTTCTTTGAAGCCTATGGTGCCATGTACAACTAAAGTTGTGAAGACAAAGAAGGAAACATCTACTTCCATTAGTAATCATGGCCTCTCAGGGAATGTTTTTCCTGAATCACTGAATTCAAAGTCAAGTGTCTTGGTTCCTTATAGTGACACGTCAGTTTCAAGAAGTGTCGAGTCTATTGAAACAATTGCCACCAACCATTCTCCCAGCAGCAGGGGTACTGTTTTTCCTGCTTCGAGTAGCATAATACAAGCTCTCAGAAGCAAGGATGTCTCTCCAAGTAGATCAGTTTCTTTGGATGAGAGCATGTCTACTTCTAACTCTGTGAAGAGTCCAGAATTTGAATATATTGACAGTGAGGACATTTCAGAAGTCAAATCTATTGAGAAGAAGACAATAAGCAGCCTCTACACTACAGACATCCCACAAATAGAAGGAATTATCCAGAAGAGAGATGTATTTACAGATACAGTGACAGGAGAAGAAATTGTTGATATCGATAACAATCTTACGGACCCACAGTTCTGTGCAACCATTGCTTCTGACATATACAAGTACTTGCTTGAATCTGAGGTAAATAGAAGGCCCGCAATGGACTTCATAGAAAGGGTGCAGAACGACATTGATGCTAGAATGCGGGCTGTTCTGATTGATTGGCTAATAGAGGTTGCCGAAGAATTCAGGCTGGTACCGGACACACTATTTCTCACTGTCAACTATGTAGATCGTTATCTTTCTGGCAATGCGATAAAAAGGCAACGATTGCAACTGCTGGGTGTTGCCTGCATGATGATTGCAGCTAAATACGAGGAGATTCATGTTCCAGAGGTGGAAGAGTTCTGTTACATTACAGACAATACATACTTCAAAGACGAGGTTTTGCAAATGGAATCTGACGTGTTGAATCATTTGAAGTTTGAAATGACAGCTCCAACAGCTATGTGCTTTTTGAGGCGCTTTTGTTCGGTTGTTGAAAGAACCAGTTGTGAGGTGCCTCCAGTGCTGTTCCAGTGCTTGGCCCACTACATTGCAGAGTTATCTCTTCTAGAATACAACATGCTTGGCTATGCCCCATCGCTGATTGCTGCTTCTGCTACTTTCTTGGCGAATTATATACTTTCTCCTTCAAAGAGGCCTTGGAATTCTACATTAAGACATTACACAAACTACCGAGCCTGTGATTTGTGTGATTGTGTTAAAGTGTTACATCACCTGTGTTGTAATGGCAGCGGTTCTAACTTAGCTGCAGTTCGAGAGAAGTATTGTCAACACAAGTACAAGTTTGTTGCCAAGAAGTACTGCCCTCCGTCAATACCTCCAGAGTTCTTCCACGATCCTAGCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04152 ko04218 ko04914 ko05161 ko05165 ko05169 ko05203 map04110 map04152 map04218 map04914 map05161 map05165 map05169 map05203
Pfam Domains
Protein Families
Cyclin_C Cyclin_N

Protein Analysis

507

Amino Acids

56.67

Weight (kDa)

6.57

Isoelectric Point (pI)

56.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 240 - 367 8.5e-47 Cyclin, N-terminal domain
Cyclin_C PF02984 369 - 490 3.6e-33 Cyclin, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 129
Acc65I GGTACC 1 cut(s) 862
AccB1I GGYRCC 4 cut(s) 46, 193, 862, 1165
AccB7I CCANNNNNTGG 1 cut(s) 1189
AccI GTMKAC 2 cut(s) 5, 485
AciI CCGC 7 cut(s) 16, 26, 56, 83, 762, 811, 1404
AclWI GGATC 1 cut(s) 1502
AcsI RAATTY 4 cut(s) 289, 512, 851, 1315
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 6 cut(s) 202, 394, 731, 864, 1451, 1472
AfiI CCNNNNNNNGG 3 cut(s) 35, 387, 1189
AflIII ACRYGT 2 cut(s) 324, 1071
AjiI CACGTC 2 cut(s) 327, 1072
AloI GAACNNNNNNTCC 2 cut(s) 680, 712
AluBI AGCT 6 cut(s) 168, 428, 974, 1103, 1112, 1418
AluI AGCT 6 cut(s) 168, 428, 974, 1103, 1112, 1418
Alw21I GWGCWC 2 cut(s) 25, 170
Alw26I GTCTC 2 cut(s) 153, 451
Alw44I GTGCAC 1 cut(s) 21
AlwI GGATC 1 cut(s) 1502
Ama87I CYCGRG 1 cut(s) 34
AoxI GGCC 5 cut(s) 75, 256, 758, 1191, 1307
ApaLI GTGCAC 1 cut(s) 21
ApeKI GCWGC 6 cut(s) 384, 576, 971, 1259, 1401, 1418
ApoI RAATTY 4 cut(s) 289, 512, 851, 1315
Asp718I GGTACC 1 cut(s) 862
AspLEI GCGC 2 cut(s) 118, 1131
AspS9I GGNCC 4 cut(s) 158, 688, 759, 1192
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 121, 1376
AvaI CYCGRG 1 cut(s) 34
AvaII GGWCC 2 cut(s) 158, 688
BaeGI GKGCMC 2 cut(s) 25, 49
BanI GGYRCC 4 cut(s) 46, 193, 862, 1165
BanII GRGCYC 1 cut(s) 170
BbsI GAAGAC 2 cut(s) 224, 572
Bbv12I GWGCWC 2 cut(s) 25, 170
BbvI GCAGC 6 cut(s) 396, 588, 983, 1246, 1405, 1413
BccI CCATC 1 cut(s) 1255
BceAI ACGGC 3 cut(s) 3, 13, 129
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 2 cut(s) 153, 451
BfaI CTAG 4 cut(s) 804, 1220, 1512, 1519
BfmI CTRYAG 2 cut(s) 588, 1419
BfoI RGCGCY 2 cut(s) 119, 1132
BglI GCCNNNNNGGC 1 cut(s) 83
BisI GCNGC 7 cut(s) 84, 385, 577, 972, 1260, 1402, 1419
BlsI GCNGC 7 cut(s) 85, 386, 578, 973, 1261, 1403, 1420
BmcAI AGTACT 2 cut(s) 731, 1472
Bme1390I CCNGG 1 cut(s) 10
Bme18I GGWCC 2 cut(s) 158, 688
BmeT110I CYCGRG 1 cut(s) 34
BmgBI CACGTC 2 cut(s) 327, 1072
BmgT120I GGNCC 4 cut(s) 158, 688, 759, 1192
BmiI GGNNCC 7 cut(s) 14, 48, 195, 312, 690, 864, 1167
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 1 cut(s) 790
BpiI GAAGAC 2 cut(s) 224, 572
BpmI CTGGAG 2 cut(s) 1155, 1476
BpuEI CTTGAG 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 672
BsaBI GATNNNNATC 2 cut(s) 678, 903
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 4 cut(s) 33, 78, 161, 1310
BsaWI WCCGGW 1 cut(s) 865
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 387, 1189
Bse1I ACTGG 4 cut(s) 160, 1155, 1172, 1183
Bse3DI GCAATG 4 cut(s) 708, 771, 922, 1200
Bse8I GATNNNNATC 2 cut(s) 678, 903
BseCI ATCGAT 1 cut(s) 672
BseDI CCNNGG 4 cut(s) 33, 78, 161, 1310
BseGI GGATG 3 cut(s) 448, 478, 594
BseJI GATNNNNATC 2 cut(s) 678, 903
BseLI CCNNNNNNNGG 3 cut(s) 35, 387, 1189
BseMI GCAATG 4 cut(s) 708, 771, 922, 1200
BseMII CTCAG 3 cut(s) 276, 445, 735
BseNI ACTGG 4 cut(s) 160, 1155, 1172, 1183
BseRI GAGGAG 1 cut(s) 998
BseSI GKGCMC 2 cut(s) 25, 49
BseXI GCAGC 6 cut(s) 396, 588, 983, 1246, 1405, 1413
BseYI CCCAGC 2 cut(s) 380, 946
BsgI GTGCAG 1 cut(s) 806
BshFI GGCC 5 cut(s) 77, 258, 760, 1193, 1309
BshNI GGYRCC 4 cut(s) 46, 193, 862, 1165
BshVI ATCGAT 1 cut(s) 672
BsiHKAI GWGCWC 2 cut(s) 25, 170
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 2 cut(s) 10, 866
BslI CCNNNNNNNGG 3 cut(s) 35, 387, 1189
BsmAI GTCTC 2 cut(s) 153, 451
BsmI GAATGC 1 cut(s) 813
BsnI GGCC 5 cut(s) 77, 258, 760, 1193, 1309
Bso31I GGTCTC 1 cut(s) 153
BsoBI CYCGRG 1 cut(s) 34
Bsp1286I GDGCHC 3 cut(s) 25, 49, 170
Bsp1407I TGTACA 1 cut(s) 200
Bsp143I GATC 3 cut(s) 458, 898, 1507
Bsp19I CCATGG 2 cut(s) 78, 161
BspACI CCGC 7 cut(s) 16, 26, 56, 83, 762, 811, 1404
BspANI GGCC 5 cut(s) 77, 258, 760, 1193, 1309
BspCNI CTCAG 3 cut(s) 275, 444, 736
BspDI ATCGAT 1 cut(s) 672
BspLI GGNNCC 7 cut(s) 14, 48, 195, 312, 690, 864, 1167
BspMAI CTGCAG 1 cut(s) 1423
BspPI GGATC 1 cut(s) 1502
BspT107I GGYRCC 4 cut(s) 46, 193, 862, 1165
BspTNI GGTCTC 1 cut(s) 153
BsrDI GCAATG 4 cut(s) 708, 771, 922, 1200
BsrGI TGTACA 1 cut(s) 200
BsrI ACTGG 4 cut(s) 160, 1155, 1172, 1183
BssECI CCNNGG 4 cut(s) 33, 78, 161, 1310
BssMI GATC 3 cut(s) 458, 898, 1507
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 3 cut(s) 78, 161, 1310
Bst4CI ACNGT 5 cut(s) 397, 530, 646, 696, 886
Bst6I CTCTTC 4 cut(s) 497, 617, 1002, 1221
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 2 cut(s) 710, 937
BstAUI TGTACA 1 cut(s) 200
BstC8I GCNNGC 3 cut(s) 762, 813, 958
BstDEI CTNAG 4 cut(s) 262, 431, 744, 1414
BstDSI CCRYGG 2 cut(s) 78, 161
BstF5I GGATG 3 cut(s) 448, 478, 594
BstH2I RGCGCY 2 cut(s) 119, 1132
BstHHI GCGC 2 cut(s) 118, 1131
BstKTI GATC 3 cut(s) 461, 901, 1510
BstMAI GTCTC 2 cut(s) 153, 451
BstMBI GATC 3 cut(s) 458, 898, 1507
BstMWI GCNNNNNNNGC 7 cut(s) 83, 193, 710, 937, 1109, 1174, 1265
BstNSI RCATGY 2 cut(s) 484, 1234
BstSCI CCNGG 1 cut(s) 8
BstSFI CTRYAG 2 cut(s) 588, 1419
BstSLI GKGCMC 2 cut(s) 25, 49
BstV1I GCAGC 6 cut(s) 396, 588, 983, 1246, 1405, 1413
BstV2I GAAGAC 2 cut(s) 224, 572
BstXI CCANNNNNNTGG 1 cut(s) 143
Bsu15I ATCGAT 1 cut(s) 672
BsuRI GGCC 5 cut(s) 77, 258, 760, 1193, 1309
BsuTUI ATCGAT 1 cut(s) 672
BtgI CCRYGG 2 cut(s) 78, 161
BtgZI GCGATG 1 cut(s) 1233
BtrI CACGTC 2 cut(s) 327, 1072
BtsCI GGATG 3 cut(s) 448, 478, 594
BtsIMutI CAGTG 6 cut(s) 284, 535, 651, 882, 1179, 1190
Cac8I GCNNGC 3 cut(s) 762, 813, 958
CfoI GCGC 2 cut(s) 118, 1131
Cfr13I GGNCC 4 cut(s) 158, 688, 759, 1192
ClaI ATCGAT 1 cut(s) 672
Csp6I GTAC 6 cut(s) 201, 393, 730, 863, 1450, 1471
CviAII CATG 8 cut(s) 79, 162, 198, 254, 481, 961, 992, 1231
CviQI GTAC 6 cut(s) 201, 393, 730, 863, 1450, 1471
DdeI CTNAG 4 cut(s) 262, 431, 744, 1414
DpnI GATC 3 cut(s) 460, 900, 1509
DpnII GATC 3 cut(s) 458, 898, 1507
Eam1104I CTCTTC 4 cut(s) 497, 617, 1002, 1221
EarI CTCTTC 4 cut(s) 497, 617, 1002, 1221
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 3 cut(s) 78, 161, 1310
Eco147I AGGCCT 1 cut(s) 1309
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 153
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 2 cut(s) 158, 688
Eco53kI GAGCTC 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 168
EcoRI GAATTC 3 cut(s) 289, 851, 1315
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 3 cut(s) 78, 161, 1310
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 3 cut(s) 78, 161, 1310
FaeI CATG 8 cut(s) 82, 165, 201, 257, 484, 964, 995, 1234
FatI CATG 8 cut(s) 78, 161, 197, 253, 480, 960, 991, 1230
FauI CCCGC 2 cut(s) 769, 804
FblI GTMKAC 2 cut(s) 5, 485
Fnu4HI GCNGC 7 cut(s) 84, 385, 577, 972, 1260, 1402, 1419
FokI GGATG 3 cut(s) 455, 485, 581
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 7 cut(s) 84, 385, 577, 972, 1260, 1402, 1419
FspBI CTAG 4 cut(s) 804, 1220, 1512, 1519
GlaI GCGC 2 cut(s) 117, 1130
GluI GCNGC 7 cut(s) 84, 385, 577, 972, 1260, 1402, 1419
GsaI CCCAGC 2 cut(s) 384, 950
GsuI CTGGAG 2 cut(s) 1155, 1476
HaeII RGCGCY 2 cut(s) 119, 1132
HaeIII GGCC 5 cut(s) 77, 258, 760, 1193, 1309
HapII CCGG 2 cut(s) 10, 866
HhaI GCGC 2 cut(s) 118, 1131
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 8 cut(s) 82, 165, 201, 257, 484, 964, 995, 1234
Hin6I GCGC 2 cut(s) 116, 1129
HinP1I GCGC 2 cut(s) 116, 1129
HincII GTYRAC 3 cut(s) 6, 889, 1442
HindII GTYRAC 3 cut(s) 6, 889, 1442
HinfI GANTC 9 cut(s) 52, 61, 281, 347, 505, 740, 988, 1064, 1078
HpaII CCGG 2 cut(s) 10, 866
HphI GGTGA 2 cut(s) 121, 1376
Hpy166II GTNNAC 5 cut(s) 6, 23, 486, 889, 1442
Hpy188I TCNGA 7 cut(s) 66, 434, 544, 718, 745, 822, 1069
Hpy188III TCNNGA 8 cut(s) 278, 336, 509, 619, 998, 1220, 1427, 1493
Hpy8I GTNNAC 5 cut(s) 6, 23, 486, 889, 1442
HpyAV CCTTC 5 cut(s) 67, 220, 602, 750, 1308
HpyCH4III ACNGT 5 cut(s) 397, 530, 646, 696, 886
HpyCH4IV ACGT 3 cut(s) 126, 326, 1071
HpyCH4V TGCA 9 cut(s) 23, 704, 787, 940, 960, 971, 1057, 1205, 1421
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 193, 710, 937, 1109, 1174, 1265
HpyF3I CTNAG 4 cut(s) 262, 431, 744, 1414
HpySE526I ACGT 3 cut(s) 126, 326, 1071
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 8 cut(s) 82, 165, 201, 257, 484, 964, 995, 1234
HspAI GCGC 2 cut(s) 116, 1129
KpnI GGTACC 1 cut(s) 866
Kzo9I GATC 3 cut(s) 458, 898, 1507
LmnI GCTCC 2 cut(s) 165, 1108
Lsp1109I GCAGC 6 cut(s) 396, 588, 983, 1246, 1405, 1413
LweI GCATC 1 cut(s) 790
MaeI CTAG 4 cut(s) 804, 1220, 1512, 1519
MaeII ACGT 3 cut(s) 126, 326, 1071
MaeIII GTNAC 7 cut(s) 38, 122, 127, 320, 646, 1016, 1377
MalI GATC 3 cut(s) 460, 900, 1509
MboI GATC 3 cut(s) 458, 898, 1507
MfeI CAATTG 1 cut(s) 360
MhlI GDGCHC 3 cut(s) 25, 49, 170
MluCI AATT 8 cut(s) 289, 360, 512, 612, 660, 851, 1282, 1315
MlyI GAGTC 2 cut(s) 356, 514
MmeI TCCRAC 1 cut(s) 1130
MseI TTAA 2 cut(s) 1325, 1371
MspA1I CMGCKG 1 cut(s) 1404
MspI CCGG 2 cut(s) 10, 866
MspR9I CCNGG 1 cut(s) 10
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 813
MwoI GCNNNNNNNGC 7 cut(s) 83, 193, 710, 937, 1109, 1174, 1265
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 2 cut(s) 78, 161
NdeII GATC 3 cut(s) 458, 898, 1507
NlaIII CATG 8 cut(s) 82, 165, 201, 257, 484, 964, 995, 1234
NlaIV GGNNCC 7 cut(s) 14, 48, 195, 312, 690, 864, 1167
NmuCI GTSAC 4 cut(s) 122, 127, 320, 646
NspI RCATGY 2 cut(s) 484, 1234
PceI AGGCCT 1 cut(s) 1309
PctI GAATGC 1 cut(s) 813
PfeI GAWTC 7 cut(s) 52, 61, 281, 740, 988, 1064, 1078
PflMI CCANNNNNTGG 1 cut(s) 1189
PkrI GCNGC 7 cut(s) 85, 386, 578, 973, 1261, 1403, 1420
PleI GAGTC 2 cut(s) 355, 513
PpsI GAGTC 2 cut(s) 355, 513
Psp124BI GAGCTC 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 380, 946
PspN4I GGNNCC 7 cut(s) 14, 48, 195, 312, 690, 864, 1167
PspPI GGNCC 4 cut(s) 158, 688, 759, 1192
PstI CTGCAG 1 cut(s) 1423
RsaI GTAC 6 cut(s) 202, 394, 731, 864, 1451, 1472
RsaNI GTAC 6 cut(s) 201, 393, 730, 863, 1450, 1471
SacI GAGCTC 1 cut(s) 170
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 1325, 1371
SatI GCNGC 7 cut(s) 84, 385, 577, 972, 1260, 1402, 1419
Sau3AI GATC 3 cut(s) 458, 898, 1507
Sau96I GGNCC 4 cut(s) 158, 688, 759, 1192
ScaI AGTACT 2 cut(s) 731, 1472
SchI GAGTC 2 cut(s) 356, 514
ScrFI CCNGG 1 cut(s) 10
SduI GDGCHC 3 cut(s) 25, 49, 170
SfaNI GCATC 1 cut(s) 790
SfcI CTRYAG 2 cut(s) 588, 1419
SinI GGWCC 2 cut(s) 158, 688
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
Sse9I AATT 8 cut(s) 289, 360, 512, 612, 660, 851, 1282, 1315
SseBI AGGCCT 1 cut(s) 1309
SsiI CCGC 7 cut(s) 16, 26, 56, 83, 762, 811, 1404
SspMI CTAG 4 cut(s) 804, 1220, 1512, 1519
SstI GAGCTC 1 cut(s) 170
StuI AGGCCT 1 cut(s) 1309
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 3 cut(s) 78, 161, 1310
TaaI ACNGT 5 cut(s) 397, 530, 646, 696, 886
TaiI ACGT 3 cut(s) 129, 329, 1074
TaqI TCGA 5 cut(s) 5, 345, 410, 672, 1426
TasI AATT 8 cut(s) 289, 360, 512, 612, 660, 851, 1282, 1315
TatI WGTACW 4 cut(s) 200, 729, 1449, 1470
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 7 cut(s) 52, 61, 281, 740, 988, 1064, 1078
Tru1I TTAA 2 cut(s) 1325, 1371
Tru9I TTAA 2 cut(s) 1325, 1371
TscAI CASTG 6 cut(s) 291, 535, 651, 889, 1179, 1190
TseFI GTSAC 4 cut(s) 122, 127, 320, 646
TseI GCWGC 6 cut(s) 384, 576, 971, 1259, 1401, 1418
Tsp45I GTSAC 4 cut(s) 122, 127, 320, 646
TspDTI ATGAA 2 cut(s) 763, 980
TspGWI ACGGA 2 cut(s) 701, 1470
TspRI CASTG 6 cut(s) 291, 535, 651, 889, 1179, 1190
Van91I CCANNNNNTGG 1 cut(s) 1189
VneI GTGCAC 1 cut(s) 21
VpaK11BI GGWCC 2 cut(s) 158, 688
XapI RAATTY 4 cut(s) 289, 512, 851, 1315
XbaI TCTAGA 1 cut(s) 1219
XceI RCATGY 2 cut(s) 484, 1234
XmiI GTMKAC 2 cut(s) 5, 485
XspI CTAG 4 cut(s) 804, 1220, 1512, 1519
ZraI GACGTC 1 cut(s) 127
ZrmI AGTACT 2 cut(s) 731, 1472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.