MD15G1344300.v1.1

Cyclin_C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001536
Physical Location & Seq
Reverse (-)
40124025 .. 40129413
5389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1344300.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGTCGACCCGGAATCGCCGTCAACCGCCATTGTCATCCTCGTTGTCGGAGACAAAGAAGCCCTCGATTTCGCATCATCCGGACAAGAAGCCCATGGCGGCCAAGACCCAAGTGCTGAAGAAGCGAACTGCTCTCGCTGACGTCACGAACCAGAGGAATAGGTGTCAAAGCGTTCCACCGACTTTAGCTTCGTCGAAGCCCATGGTGCCATGTACAAATAAAATTGCTAAGACGATTGCCAATAAGACGACTGCTAAGACGACAAAGGAAGTGTCATCTTGCACGAGTAATACTGGCTTGTCAGGGAATGTCTTTCAAGCATCGTTGAGTGCAAAATCAAACATCCTGGTTCCTTTCAGTGATACATCATTCTCAGGAACTGATCCGGCTATCAAAGTAATTTGCACTGATCCTTCTCCAAGCAGCAGTGGTACTGTCCTTCCTGCTCTCAGTTGCATAAATCATGCATTCAGAAGCTGGGATGCATCTCCAAGCAGATCAGTTAGTGCTTCAGTTTCTTTGGATGAGAGCATGTCTACATGTGATTCGTTAAATTCTCCAGAATTTGAATATATTGACAATGAGGATGTTTCAGATGTTAAATGGATTGAGAAGAAAACAACTAAGAGCCTATTCATTTCGAACCATCCAGGAAAAGAAGCCAATATCTGGAAGAAAGATATATTTTTCGATATGGAGGCAACAGATAAAGTCGTTGCTATGGATGACAATTTTATGGATCCACAGTTTTGTGCAGCCATTGCTCCTGACATATACAAGCACTTGCGTGAATCTGAGGAAAATAGAAGGCCCTCCATGGACTTTATGGAAACAATCCAGAAAGACATTAATGCCGGCATGCGTGCTATTCTGATTGATTGGCTAGTAGAGGTTGCTGAAGAGTTCAGGCTTGTACCAGAAACTCTATTTCTGACTATCAACTATGTAGACCGTTATCTTTCTGGCAATGTGATGAATAGAAAACAATTGCAGTTGCTGGGGGTTGCCTGCATGATGATTGCAGCTAAATACGAGGAGATTGTTGCTCCCGAGGTGGAACAGTTCTGTTACATTACGGACAACACATACACCAAAAAGGAGGTTTTGCAAATGGAATCCTCTGTCCTGAATCACTTGAAGTTTGAAATGACAGCTCCAACGGCCTTGTGCTTTTTGAGGCGATTTTGTTTTGCTGCTCAAAAGACCAGTAAGGTTCCATCAGAGCATTTCCGGTGCTTGGCCTCCTACATCACAGAGTTGTCTCTTCTAGAATATAGCATGCTTTGTTATGCCCCATCTCTAATAGCTGCTTCTGCTGCCTTCTTGGCAAAGTATATTCTTTCCCCTTTGAAAAAACCTTGGAATTCTACGTTGAGACACTACACACTTTACCAAGCCTCTGATTTGTTTGATTGTGTAAAAGCGCTGCATCAACTGTGTTGTAATGGCTGTGGTTCAAACTTACCTGCAGTTAGGGAGAAGTACTGTCAACATAAATACAAGTTCGTGGCGAAGAAGTACTGTCCCCCATCAATACCTCCAGAGTTCTTCCAGGATTTAAGCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04152 ko04218 ko04914 ko05161 ko05165 ko05169 ko05203 map04110 map04152 map04218 map04914 map05161 map05165 map05169 map05203
Pfam Domains
Protein Families
Cyclin_C Cyclin_N

Protein Analysis

523

Amino Acids

58.47

Weight (kDa)

8.39

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 257 - 384 9.2e-49 Cyclin, N-terminal domain
Cyclin_C PF02984 386 - 506 2.4e-32 Cyclin, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc36I ACCTGC 1 cut(s) 1474
AccB1I GGYRCC 1 cut(s) 205
AccB7I CCANNNNNTGG 1 cut(s) 669
AccI GTMKAC 3 cut(s) 5, 536, 948
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 2 cut(s) 26, 98
AclWI GGATC 4 cut(s) 377, 404, 734, 747
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 3 cut(s) 553, 563, 1363
AcuI CTGAAG 3 cut(s) 137, 495, 918
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 5 cut(s) 214, 433, 915, 1484, 1520
AfeI AGCGCT 1 cut(s) 1425
AfiI CCNNNNNNNGG 2 cut(s) 669, 1237
AflIII ACRYGT 1 cut(s) 539
AgsI TTSAA 6 cut(s) 317, 569, 1138, 1145, 1351, 1458
AjnI CCWGG 3 cut(s) 345, 649, 1551
AleI CACNNNNGTG 1 cut(s) 786
AluBI AGCT 5 cut(s) 188, 477, 1025, 1154, 1307
AluI AGCT 5 cut(s) 188, 477, 1025, 1154, 1307
Alw26I GTCTC 3 cut(s) 44, 1266, 1369
AlwI GGATC 4 cut(s) 377, 404, 734, 747
AlwNI CAGNNNCTG 3 cut(s) 380, 477, 997
Ama87I CYCGRG 1 cut(s) 1049
Aor13HI TCCGGA 1 cut(s) 79
Aor51HI AGCGCT 1 cut(s) 1425
AoxI GGCC 4 cut(s) 99, 809, 1161, 1239
ApeKI GCWGC 7 cut(s) 423, 755, 1022, 1193, 1307, 1316, 1426
ApoI RAATTY 3 cut(s) 553, 563, 1363
ArsI GACNNNNNNTTYG 4 cut(s) 172, 204, 257, 289
AseI ATTAAT 1 cut(s) 849
AspLEI GCGC 1 cut(s) 1426
AspS9I GGNCC 1 cut(s) 810
AsuC2I CCSGG 1 cut(s) 10
AsuII TTCGAA 1 cut(s) 641
AvaI CYCGRG 1 cut(s) 1049
BamHI GGATCC 1 cut(s) 739
BanI GGYRCC 1 cut(s) 205
BauI CACGAG 1 cut(s) 283
BbvI GCAGC 7 cut(s) 435, 767, 1034, 1180, 1294, 1303, 1413
BccI CCATC 4 cut(s) 654, 1225, 1303, 1537
BceAI ACGGC 2 cut(s) 3, 1176
BciT130I CCWGG 3 cut(s) 347, 651, 1553
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 3 cut(s) 44, 1266, 1369
BfaI CTAG 3 cut(s) 884, 1268, 1567
BfmI CTRYAG 1 cut(s) 1467
BfoI RGCGCY 1 cut(s) 1427
BfuAI ACCTGC 1 cut(s) 1474
BglI GCCNNNNNGGC 1 cut(s) 1325
BisI GCNGC 8 cut(s) 99, 424, 756, 1023, 1194, 1308, 1317, 1427
BlsI GCNGC 8 cut(s) 100, 425, 757, 1024, 1195, 1309, 1318, 1428
BmcAI AGTACT 2 cut(s) 1484, 1520
Bme1390I CCNGG 4 cut(s) 10, 347, 651, 1553
BmeT110I CYCGRG 1 cut(s) 1049
BmgT120I GGNCC 1 cut(s) 810
BmiI GGNNCC 4 cut(s) 207, 351, 741, 1215
BmrFI CCNGG 4 cut(s) 10, 347, 651, 1553
BmsI GCATC 5 cut(s) 82, 329, 472, 494, 1438
BpmI CTGGAG 2 cut(s) 543, 1524
Bpu14I TTCGAA 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 5 cut(s) 93, 201, 816, 1050, 1358
BsaWI WCCGGW 2 cut(s) 79, 1230
Bsc4I CCNNNNNNNGG 2 cut(s) 669, 1237
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 2 cut(s) 298, 1206
Bse3DI GCAATG 2 cut(s) 759, 973
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 3 cut(s) 347, 651, 1553
BseDI CCNNGG 5 cut(s) 93, 201, 816, 1050, 1358
BseGI GGATG 8 cut(s) 35, 76, 342, 487, 529, 592, 646, 730
BseLI CCNNNNNNNGG 2 cut(s) 669, 1237
BseMI GCAATG 2 cut(s) 759, 973
BseMII CTCAG 3 cut(s) 387, 463, 786
BseNI ACTGG 2 cut(s) 298, 1206
BseRI GAGGAG 1 cut(s) 1049
BseXI GCAGC 7 cut(s) 435, 767, 1034, 1180, 1294, 1303, 1413
BseYI CCCAGC 2 cut(s) 477, 997
BsgI GTGCAG 1 cut(s) 774
BshFI GGCC 4 cut(s) 101, 811, 1163, 1241
BshNI GGYRCC 1 cut(s) 205
BsiHKCI CYCGRG 1 cut(s) 1049
BsiSI CCGG 5 cut(s) 10, 80, 386, 855, 1231
BslFI GGGAC 1 cut(s) 1509
BslI CCNNNNNNNGG 2 cut(s) 669, 1237
BsmAI GTCTC 3 cut(s) 44, 1266, 1369
BsmFI GGGAC 1 cut(s) 1509
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 4 cut(s) 101, 811, 1163, 1241
BsoBI CYCGRG 1 cut(s) 1049
Bsp119I TTCGAA 1 cut(s) 641
Bsp13I TCCGGA 1 cut(s) 79
Bsp1407I TGTACA 1 cut(s) 212
Bsp143I GATC 4 cut(s) 382, 409, 497, 739
Bsp19I CCATGG 3 cut(s) 93, 201, 816
BspACI CCGC 2 cut(s) 26, 98
BspANI GGCC 4 cut(s) 101, 811, 1163, 1241
BspCNI CTCAG 3 cut(s) 386, 462, 787
BspEI TCCGGA 1 cut(s) 79
BspLI GGNNCC 4 cut(s) 207, 351, 741, 1215
BspMAI CTGCAG 1 cut(s) 1471
BspMI ACCTGC 1 cut(s) 1474
BspPI GGATC 4 cut(s) 377, 404, 734, 747
BspT104I TTCGAA 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 205
BsrDI GCAATG 2 cut(s) 759, 973
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 212
BsrI ACTGG 2 cut(s) 298, 1206
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 5 cut(s) 93, 201, 816, 1050, 1358
BssMI GATC 4 cut(s) 382, 409, 497, 739
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 283
BssT1I CCWWGG 4 cut(s) 93, 201, 816, 1358
Bst2BI CACGAG 1 cut(s) 283
Bst2UI CCWGG 3 cut(s) 347, 651, 1553
Bst4CI ACNGT 7 cut(s) 436, 747, 953, 1062, 1437, 1487, 1523
Bst6I CTCTTC 2 cut(s) 894, 1269
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 761
BstAUI TGTACA 1 cut(s) 212
BstBI TTCGAA 1 cut(s) 641
BstC8I GCNNGC 5 cut(s) 856, 860, 864, 1009, 1280
BstDEI CTNAG 6 cut(s) 228, 255, 373, 449, 624, 795
BstDSI CCRYGG 3 cut(s) 93, 201, 816
BstF5I GGATG 8 cut(s) 35, 76, 342, 487, 529, 592, 646, 730
BstH2I RGCGCY 1 cut(s) 1427
BstHHI GCGC 1 cut(s) 1426
BstKTI GATC 4 cut(s) 385, 412, 500, 742
BstMAI GTCTC 3 cut(s) 44, 1266, 1369
BstMBI GATC 4 cut(s) 382, 409, 497, 739
BstMWI GCNNNNNNNGC 7 cut(s) 121, 205, 761, 1160, 1313, 1316, 1325
BstNI CCWGG 3 cut(s) 347, 651, 1553
BstNSI RCATGY 4 cut(s) 535, 543, 862, 1282
BstSCI CCNGG 4 cut(s) 8, 345, 649, 1551
BstSFI CTRYAG 1 cut(s) 1467
BstV1I GCAGC 7 cut(s) 435, 767, 1034, 1180, 1294, 1303, 1413
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
BsuRI GGCC 4 cut(s) 101, 811, 1163, 1241
BtgI CCRYGG 3 cut(s) 93, 201, 816
BtsCI GGATG 8 cut(s) 35, 76, 342, 487, 529, 592, 646, 730
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 3 cut(s) 364, 405, 433
BveI ACCTGC 1 cut(s) 1474
Cac8I GCNNGC 5 cut(s) 856, 860, 864, 1009, 1280
CaiI CAGNNNCTG 3 cut(s) 380, 477, 997
CfoI GCGC 1 cut(s) 1426
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 1 cut(s) 810
Csp6I GTAC 5 cut(s) 213, 432, 914, 1483, 1519
CviQI GTAC 5 cut(s) 213, 432, 914, 1483, 1519
DdeI CTNAG 6 cut(s) 228, 255, 373, 449, 624, 795
DpnI GATC 4 cut(s) 384, 411, 499, 741
DpnII GATC 4 cut(s) 382, 409, 497, 739
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 2 cut(s) 894, 1269
EarI CTCTTC 2 cut(s) 894, 1269
Eco130I CCWWGG 4 cut(s) 93, 201, 816, 1358
Eco47III AGCGCT 1 cut(s) 1425
Eco57I CTGAAG 3 cut(s) 137, 495, 918
Eco88I CYCGRG 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 810
EcoRI GAATTC 1 cut(s) 1363
EcoRII CCWGG 3 cut(s) 345, 649, 1551
EcoT14I CCWWGG 4 cut(s) 93, 201, 816, 1358
EcoT22I ATGCAT 2 cut(s) 469, 487
ErhI CCWWGG 4 cut(s) 93, 201, 816, 1358
FaqI GGGAC 1 cut(s) 1509
FblI GTMKAC 3 cut(s) 5, 536, 948
Fnu4HI GCNGC 8 cut(s) 99, 424, 756, 1023, 1194, 1308, 1317, 1427
FokI GGATG 8 cut(s) 22, 63, 329, 494, 536, 599, 633, 737
Fsp4HI GCNGC 8 cut(s) 99, 424, 756, 1023, 1194, 1308, 1317, 1427
FspBI CTAG 3 cut(s) 884, 1268, 1567
GlaI GCGC 1 cut(s) 1425
GluI GCNGC 8 cut(s) 99, 424, 756, 1023, 1194, 1308, 1317, 1427
GsaI CCCAGC 2 cut(s) 481, 1001
GsuI CTGGAG 2 cut(s) 543, 1524
HaeII RGCGCY 1 cut(s) 1427
HaeIII GGCC 4 cut(s) 101, 811, 1163, 1241
HapII CCGG 5 cut(s) 10, 80, 386, 855, 1231
HhaI GCGC 1 cut(s) 1426
Hin1I GRCGYC 1 cut(s) 141
Hin6I GCGC 1 cut(s) 1424
HinP1I GCGC 1 cut(s) 1424
HincII GTYRAC 3 cut(s) 6, 23, 1490
HindII GTYRAC 3 cut(s) 6, 23, 1490
HinfI GANTC 5 cut(s) 13, 545, 791, 1115, 1129
HpaII CCGG 5 cut(s) 10, 80, 386, 855, 1231
Hpy166II GTNNAC 5 cut(s) 6, 23, 537, 949, 1490
Hpy188I TCNGA 8 cut(s) 49, 473, 595, 796, 873, 933, 1222, 1402
Hpy8I GTNNAC 5 cut(s) 6, 23, 537, 949, 1490
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 4 cut(s) 423, 449, 801, 1330
HpyCH4III ACNGT 7 cut(s) 436, 747, 953, 1062, 1437, 1487, 1523
HpyCH4IV ACGT 2 cut(s) 141, 1370
HpyF10VI GCNNNNNNNGC 7 cut(s) 121, 205, 761, 1160, 1313, 1316, 1325
HpyF3I CTNAG 6 cut(s) 228, 255, 373, 449, 624, 795
HpySE526I ACGT 2 cut(s) 141, 1370
Hsp92I GRCGYC 1 cut(s) 141
HspAI GCGC 1 cut(s) 1424
Kpn2I TCCGGA 1 cut(s) 79
KroI GCCGGC 1 cut(s) 854
KroNI GCCGGC 1 cut(s) 856
Kzo9I GATC 4 cut(s) 382, 409, 497, 739
LmnI GCTCC 3 cut(s) 769, 1051, 1159
Lsp1109I GCAGC 7 cut(s) 435, 767, 1034, 1180, 1294, 1303, 1413
LweI GCATC 5 cut(s) 82, 329, 472, 494, 1438
MaeI CTAG 3 cut(s) 884, 1268, 1567
MaeII ACGT 2 cut(s) 141, 1370
MaeIII GTNAC 2 cut(s) 142, 1067
MalI GATC 4 cut(s) 384, 411, 499, 741
MboI GATC 4 cut(s) 382, 409, 497, 739
MboII GAAGA 7 cut(s) 130, 625, 685, 911, 1256, 1525, 1540
MfeI CAATTG 1 cut(s) 986
MflI RGATCY 1 cut(s) 739
MluCI AATT 7 cut(s) 222, 399, 553, 563, 730, 986, 1363
MmeI TCCRAC 2 cut(s) 27, 1181
Mph1103I ATGCAT 2 cut(s) 469, 487
MroI TCCGGA 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 854
MseI TTAA 4 cut(s) 551, 600, 849, 1559
MslI CAYNNNNRTG 1 cut(s) 786
MspI CCGG 5 cut(s) 10, 80, 386, 855, 1231
MspR9I CCNGG 4 cut(s) 10, 347, 651, 1553
MunI CAATTG 1 cut(s) 986
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 3 cut(s) 347, 651, 1553
MwoI GCNNNNNNNGC 7 cut(s) 121, 205, 761, 1160, 1313, 1316, 1325
NaeI GCCGGC 1 cut(s) 856
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 3 cut(s) 93, 201, 816
NdeII GATC 4 cut(s) 382, 409, 497, 739
NgoMIV GCCGGC 1 cut(s) 854
NlaIV GGNNCC 4 cut(s) 207, 351, 741, 1215
NmuCI GTSAC 1 cut(s) 142
NsiI ATGCAT 2 cut(s) 469, 487
NspI RCATGY 4 cut(s) 535, 543, 862, 1282
NspV TTCGAA 1 cut(s) 641
OliI CACNNNNGTG 1 cut(s) 786
PaeI GCATGC 2 cut(s) 862, 1282
PciI ACATGT 1 cut(s) 539
PctI GAATGC 1 cut(s) 467
PdiI GCCGGC 1 cut(s) 856
PfeI GAWTC 5 cut(s) 13, 545, 791, 1115, 1129
PflMI CCANNNNNTGG 1 cut(s) 669
PfoI TCCNGGA 2 cut(s) 649, 1551
PkrI GCNGC 8 cut(s) 100, 425, 757, 1024, 1195, 1309, 1318, 1428
PscI ACATGT 1 cut(s) 539
PshBI ATTAAT 1 cut(s) 849
Psp6I CCWGG 3 cut(s) 345, 649, 1551
PspFI CCCAGC 2 cut(s) 477, 997
PspGI CCWGG 3 cut(s) 345, 649, 1551
PspN4I GGNNCC 4 cut(s) 207, 351, 741, 1215
PspPI GGNCC 1 cut(s) 810
PstI CTGCAG 1 cut(s) 1471
PstNI CAGNNNCTG 3 cut(s) 380, 477, 997
PsuI RGATCY 1 cut(s) 739
RsaI GTAC 5 cut(s) 214, 433, 915, 1484, 1520
RsaNI GTAC 5 cut(s) 213, 432, 914, 1483, 1519
RseI CAYNNNNRTG 1 cut(s) 786
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 551, 600, 849, 1559
SatI GCNGC 8 cut(s) 99, 424, 756, 1023, 1194, 1308, 1317, 1427
Sau3AI GATC 4 cut(s) 382, 409, 497, 739
Sau96I GGNCC 1 cut(s) 810
ScaI AGTACT 2 cut(s) 1484, 1520
ScrFI CCNGG 4 cut(s) 10, 347, 651, 1553
SfaNI GCATC 5 cut(s) 82, 329, 472, 494, 1438
SfcI CTRYAG 1 cut(s) 1467
SfuI TTCGAA 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 786
SphI GCATGC 2 cut(s) 862, 1282
Sse9I AATT 7 cut(s) 222, 399, 553, 563, 730, 986, 1363
SsiI CCGC 2 cut(s) 26, 98
SspMI CTAG 3 cut(s) 884, 1268, 1567
StyD4I CCNGG 4 cut(s) 8, 345, 649, 1551
StyI CCWWGG 4 cut(s) 93, 201, 816, 1358
TaaI ACNGT 7 cut(s) 436, 747, 953, 1062, 1437, 1487, 1523
TaiI ACGT 2 cut(s) 144, 1373
TaqI TCGA 5 cut(s) 5, 65, 194, 641, 690
TasI AATT 7 cut(s) 222, 399, 553, 563, 730, 986, 1363
TatI WGTACW 3 cut(s) 212, 1482, 1518
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 5 cut(s) 13, 545, 791, 1115, 1129
Tru1I TTAA 4 cut(s) 551, 600, 849, 1559
Tru9I TTAA 4 cut(s) 551, 600, 849, 1559
TscAI CASTG 3 cut(s) 364, 412, 433
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 7 cut(s) 423, 755, 1022, 1193, 1307, 1316, 1426
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 2 cut(s) 625, 989
TspGWI ACGGA 1 cut(s) 1091
TspRI CASTG 3 cut(s) 364, 412, 433
Van91I CCANNNNNTGG 1 cut(s) 669
VspI ATTAAT 1 cut(s) 849
XapI RAATTY 3 cut(s) 553, 563, 1363
XbaI TCTAGA 1 cut(s) 1267
XceI RCATGY 4 cut(s) 535, 543, 862, 1282
XcmI CCANNNNNNNNNTGG 1 cut(s) 823
XmiI GTMKAC 3 cut(s) 5, 536, 948
XspI CTAG 3 cut(s) 884, 1268, 1567
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 2 cut(s) 1484, 1520
Zsp2I ATGCAT 2 cut(s) 469, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.