Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002861

Physical Location & Seq

Genomic Coordinates Reverse (-)

25430198 .. 25432477

Gene Structure 2280 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g34120.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGGTTTCCACCCTATGATCTCTTTGTTGGTGTTTACCATACTGCGAATCAACAATGCAGTTTCAAATGCTGAGGAAACCCAGACATACATCATACACATGGATCGTTTCCACAAACCCAAGTCTTTCGCAACCCATGAAGCATGGCACAGGTCTACCTTAAATTCGTTAATGTCATCTTCATCTCTGGAAGATCAAGAAAATGAAATGTTGTTGTACTCGTACAACCATGTCATGCATGGCTTCAGTGCCAGGCTCACAGCCTCTCAACTGTCTGAATTGGAGAAATCTCCGGCTCATATTGCTACATACCCGGAGTCTTTTGGCAAGCTCTTCACAACACACAGCTCCAAGTTTCTAGGACTAAGACAAAACTCTGGCTTATTGCTTGCTTCCTCATCGGGCCAAGATGTGATCATAGGGATCATAGATACCGGAGTTTGGCCAGAGAGCGAGAGTTTTAGTGACAGGGGAATGTCTGAAGTGCCGAAAAGATGGAAAGGCGCATGCGAGAATGGAACGGATTTCAGCCCATCCCTCTGCAACAACAAGCTTATTGGGGCTCGATCCTTCAGCAAAGGGCTGGAAGCAGCAGGGATCAAAATATCCACAAAAGATGACTTTGATTCACCAAGGGACTGGTTTGGTCATGGAACTCACACTTCGTCAACAGCTGCAGGTAACTATGTACTTGGCGCAAGTCATTTCGGATATGCAAAAGGCACAGCAAGAGGGGTGGCGCCACGAGCCCATGTTGCCATGTACAAGGTCCTCTGGGCAACAGACTCCGATCAGAGTGCAGCTAGTGATGTGCTTGCAGGCATGGACCAGGCAATTTCTGATGGTGTTGATATAATGTCACTGTCTCTTGGCTTTGAGTCCCTTCCTTATTTCAATGATGTCATTGCCATTGCTTCTCTCTCTGCAATAGAGAAGGGAATTGTCGTGGTCTGTGCTGCTGGCAACGACGGCGCTTACAGTTCCATACACAATGGAGCACCCTGGATCACAACAGTAGGGGCCGGCACGCTTGATCGCACCTTCACTGCTACACTGACTCTGGATAATGGTTTGACAGTTGAAGGAATATCATACTTCCCAGAGAGTGTTTACATTACTGATGCGCCATTGTACTACGGCAAAGACAATGCAAACAAAGAAATATGCAATTATGGGGCGCTGGATCATAAAGAAGTGAAGGGAAAGGTAGTCCTCTGTGATAATACTACTGAGATTGATGTGGAAGGACAAAAGGAAGAGGTACAGAGGGCAGGTGGTTATGCCGGAATCTTCATGACAGATCTCTCATTATTAGACCCAGAAGACTTCTCCATTCCTGCTATCATTGTTCCATTTGCTACAGGTGCCTTGATTAGAGAGTATGCAACACAGGCCGATACAACTCGAGTTAAGACACTGAGTTTTGTGCACACAAAGTTGGGTACAAAACCATCACCGGAAGTGGCGTATTTCTCTTCAAGAGGACCAGACCCGGTCACTCCAAGTATTCTAAAACCAGACGTTCTTGCTCCAGGAGTCGACGTGCTTGCTGCAGTTGCACCAAACAAACCATGCATGCGAGTAAACAATTACGATTTAGTAACAGATTATGAACTGTACTCAGGAACATCAATGGCGGCACCCCATGTCGCCGGAGTGGCAGCTCTGCTAAAGGCTGTTCACCGAGAATGGAGTCCAGCAGCCATCCGATCAGCCATCATGACGACAGCATACTCAACCGGGAAAAATTCTCAGACCACCATACTCAACCAATGGTCGGGTCTTCCCGCCTCGCCTTTAGATTTTGGTGCGGGACACATCAACCCAAACAAAGCCATGAACCCTGGACTCATCTATGACATGGATCTGCAGGATTACATTGAGTTTCTATGTGGCCTTGGGTATAATGCCAAGCAAATGAAAGCTGTTATCAGACGAAGCCATTGGTATTGCAGCTCAACACCAGCTGACCTAAACTACCCTTCTTTCATGGCCATTTTTAACGGAACAGACTCTCCTAAAGCCAAAACCTTCAGCAGGGTTGTGACAAACGTGGGGGATGAAATATCAACCTACAGAGCATATTTAGAGGTTCCGAAAGGAATGAGGATTACAGTAGAGCCTAGTACTCTAGTATTCACTGGCAAAAACCAAGAGCAAGGTTTCAGCTTGAGTGTAGAGGTCGATAGCGATGCCCCGAAGGTGATCTATGGTTATCTCGGATGGATTGACCAGTATAACCACATCGTATCAAGCCCTGTAGTAGCAATCAACTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

760

Amino Acids

82.3

Weight (kDa)

5.51

Isoelectric Point (pI)

39.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	29 - 107	2.1e-17	Peptidase inhibitor I9
Peptidase_S8	PF00082	135 - 582	1.4e-46	Subtilase family
fn3_6	PF17766	657 - 755	2.6e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002861)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34120 FvH4_2g34130 FvH4_2g34150 FvH4_2g34210 FvH4_2g34211 FvH4_2g34212
malus_domestica	MD05G1275700.v1.1 MD05G1275800.v1.1 MD08G1098000.v1.1 MD15G1080200.v1.1 MD15G1080300.v1.1
prunus_persica	Prupe.1G433100_v2.0.a1 Prupe.1G433200_v2.0.a1
pyrus_communis	pycom15g07460
rosa_chinensis	RchiOBHm_Chr6g0308201 RchiOBHm_Chr6g0308211 RchiOBHm_Chr6g0308221
rosa_laevigata	RLG00000010657 RLG00000010658 RLG00000010659
rosa_multiflora	Rmu_sc0005706.1_g000017 Rmu_sc0005706.1_g000018 Rmu_ssc0000355.1_g000001 Rmu_ssc0000355.1_g000006
rosa_roxburghii	Rroxscaffold_7G00160340 Rroxscaffold_7G00160360 Rroxscaffold_7G00160440 Rroxscaffold_7G00160450
rosa_rugosa	Rorug01G0005200 Rorug01G0066300 Rorug06G0364700 Rorug06G0364800 Rorug06G0364900
rosa_samantha	Rh6DG475900 Rh6DG476100 Rh6DG476300
rosa_wichuraiana	Rw6G041330 Rw6G041340 Rw6G041350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1262
Acc36I	ACCTGC	2 cut(s)	668, 1262
AccB1I	GGYRCC	3 cut(s)	739, 1364, 1639
AccI	GTMKAC	2 cut(s)	155, 1539
AciI	CCGC	3 cut(s)	1637, 1788, 1811
AclWI	GGATC	7 cut(s)	111, 431, 561, 605, 1013, 1191, 1872
AcoI	YGGCCR	2 cut(s)	443, 1992
AcsI	RAATTY	2 cut(s)	163, 1747
AcuI	CTGAAG	4 cut(s)	229, 501, 556, 2017
AcyI	GRCGYC	1 cut(s)	740
AfaI	GTAC	9 cut(s)	218, 224, 690, 764, 1133, 1263, 1444, 1619, 2128
AfiI	CCNNNNNNNGG	3 cut(s)	441, 1777, 2037
AgsI	TTSAA	4 cut(s)	66, 895, 1082, 1479
AjiI	CACGTC	1 cut(s)	1543
AjnI	CCWGG	5 cut(s)	251, 828, 1001, 1531, 1843
AloI	GAACNNNNNNTCC	2 cut(s)	1999, 2031
Alw21I	GWGCWC	2 cut(s)	1000, 1431
Alw26I	GTCTC	1 cut(s)	870
Alw44I	GTGCAC	1 cut(s)	1427
AlwI	GGATC	7 cut(s)	111, 431, 561, 605, 1013, 1191, 1872
Ama87I	CYCGRG	1 cut(s)	1404
AoxI	GGCC	6 cut(s)	403, 443, 1020, 1392, 1894, 1992
ApaLI	GTGCAC	1 cut(s)	1427
ApeKI	GCWGC	8 cut(s)	590, 674, 800, 956, 1550, 1661, 1700, 1953
ApoI	RAATTY	2 cut(s)	163, 1747
AspLEI	GCGC	6 cut(s)	506, 698, 742, 974, 1126, 1180
AspS9I	GGNCC	5 cut(s)	403, 769, 826, 1020, 1484
AsuC2I	CCSGG	3 cut(s)	314, 1493, 1741
AsuHPI	GGTGA	4 cut(s)	621, 1446, 1673, 2215
AvaI	CYCGRG	1 cut(s)	1404
AvaII	GGWCC	3 cut(s)	769, 826, 1484
BaeGI	GKGCMC	1 cut(s)	1431
BaeI	ACNNNNGTAYC	2 cut(s)	423, 456
BalI	TGGCCA	2 cut(s)	445, 1994
BanI	GGYRCC	3 cut(s)	739, 1364, 1639
BanII	GRGCYC	2 cut(s)	565, 751
BauI	CACGAG	1 cut(s)	744
BbsI	GAAGAC	2 cut(s)	1329, 1775
Bbv12I	GWGCWC	2 cut(s)	1000, 1431
BbvCI	CCTCAGC	1 cut(s)	72
BbvI	GCAGC	8 cut(s)	602, 661, 812, 943, 1537, 1673, 1712, 1965
BccI	CCATC	7 cut(s)	489, 541, 836, 1459, 1712, 1724, 2217
BceAI	ACGGC	2 cut(s)	985, 1153
BcgI	CGANNNNNNTGC	6 cut(s)	779, 813, 1529, 1563, 2174, 2208
BciT130I	CCWGG	5 cut(s)	253, 830, 1003, 1533, 1845
BclI	TGATCA	1 cut(s)	414
BcnI	CCSGG	3 cut(s)	314, 1493, 1741
BcoDI	GTCTC	1 cut(s)	870
BfaI	CTAG	5 cut(s)	359, 804, 2124, 2132, 2278
BfmI	CTRYAG	6 cut(s)	675, 1359, 1551, 1868, 2074, 2259
BfoI	RGCGCY	3 cut(s)	743, 975, 1181
BfuAI	ACCTGC	2 cut(s)	668, 1262
BglI	GCCNNNNNGGC	1 cut(s)	1658
BglII	AGATCT	1 cut(s)	1300
BisI	GCNGC	9 cut(s)	591, 675, 801, 957, 1551, 1638, 1662, 1701, 1954
BlsI	GCNGC	9 cut(s)	592, 676, 802, 958, 1552, 1639, 1663, 1702, 1955
BmcAI	AGTACT	1 cut(s)	2128
Bme1390I	CCNGG	8 cut(s)	253, 314, 830, 1003, 1493, 1533, 1741, 1845
Bme18I	GGWCC	3 cut(s)	769, 826, 1484
BmeT110I	CYCGRG	1 cut(s)	1404
BmgBI	CACGTC	1 cut(s)	1543
BmgT120I	GGNCC	5 cut(s)	403, 769, 826, 1020, 1484
BmiI	GGNNCC	5 cut(s)	741, 1021, 1366, 1641, 2094
BmrFI	CCNGG	8 cut(s)	253, 314, 830, 1003, 1493, 1533, 1741, 1845
BmsI	GCATC	2 cut(s)	1111, 2182
BpiI	GAAGAC	2 cut(s)	1329, 1775
BpmI	CTGGAG	1 cut(s)	1515
Bpu10I	CCTNAGC	1 cut(s)	72
BpuEI	CTTGAG	1 cut(s)	2191
BpuMI	CCSGG	3 cut(s)	314, 1493, 1741
BsaHI	GRCGYC	1 cut(s)	740
BsaJI	CCNNGG	4 cut(s)	632, 1001, 1843, 1897
BsaWI	WCCGGW	2 cut(s)	434, 1456
BsaXI	ACNNNNNCTCC	4 cut(s)	1527, 1557, 1999, 2029
Bsc4I	CCNNNNNNNGG	3 cut(s)	441, 1777, 2037
Bse118I	RCCGGY	1 cut(s)	1022
Bse1I	ACTGG	3 cut(s)	644, 2146, 2233
Bse3DI	GCAATG	2 cut(s)	903, 909
BseBI	CCWGG	5 cut(s)	253, 830, 1003, 1533, 1845
BseDI	CCNNGG	4 cut(s)	632, 1001, 1843, 1897
BseGI	GGATG	4 cut(s)	533, 1704, 2065, 2228
BseLI	CCNNNNNNNGG	3 cut(s)	441, 1777, 2037
BseMI	GCAATG	2 cut(s)	903, 909
BseMII	CTCAG	5 cut(s)	63, 1221, 1409, 1635, 1766
BseNI	ACTGG	3 cut(s)	644, 2146, 2233
BseSI	GKGCMC	1 cut(s)	1431
BseXI	GCAGC	8 cut(s)	602, 661, 812, 943, 1537, 1673, 1712, 1965
BsgI	GTGCAG	1 cut(s)	819
BshFI	GGCC	6 cut(s)	405, 445, 1022, 1394, 1896, 1994
BshNI	GGYRCC	3 cut(s)	739, 1364, 1639
BsiHKAI	GWGCWC	2 cut(s)	1000, 1431
BsiHKCI	CYCGRG	1 cut(s)	1404
BsiSI	CCGG	9 cut(s)	293, 314, 435, 1023, 1284, 1457, 1493, 1653, 1740
BslFI	GGGAC	3 cut(s)	650, 865, 1827
BslI	CCNNNNNNNGG	3 cut(s)	441, 1777, 2037
BsmAI	GTCTC	1 cut(s)	870
BsmFI	GGGAC	3 cut(s)	650, 865, 1827
BsnI	GGCC	6 cut(s)	405, 445, 1022, 1394, 1896, 1994
BsoBI	CYCGRG	1 cut(s)	1404
Bsp1286I	GDGCHC	4 cut(s)	565, 751, 1000, 1431
Bsp1407I	TGTACA	1 cut(s)	762
BspACI	CCGC	3 cut(s)	1637, 1788, 1811
BspANI	GGCC	6 cut(s)	405, 445, 1022, 1394, 1896, 1994
BspCNI	CTCAG	5 cut(s)	64, 1222, 1410, 1634, 1765
BspHI	TCATGA	2 cut(s)	1293, 1719
BspLI	GGNNCC	5 cut(s)	741, 1021, 1366, 1641, 2094
BspMAI	CTGCAG	3 cut(s)	679, 1555, 1872
BspMI	ACCTGC	2 cut(s)	668, 1262
BspPI	GGATC	7 cut(s)	111, 431, 561, 605, 1013, 1191, 1872
BspQI	GCTCTTC	1 cut(s)	338
BspT107I	GGYRCC	3 cut(s)	739, 1364, 1639
BsrDI	GCAATG	2 cut(s)	903, 909
BsrFI	RCCGGY	1 cut(s)	1022
BsrGI	TGTACA	1 cut(s)	762
BsrI	ACTGG	3 cut(s)	644, 2146, 2233
BssAI	RCCGGY	1 cut(s)	1022
BssECI	CCNNGG	4 cut(s)	632, 1001, 1843, 1897
BssNI	GRCGYC	1 cut(s)	740
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	2 cut(s)	632, 1897
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	5 cut(s)	253, 830, 1003, 1533, 1845
Bst4CI	ACNGT	7 cut(s)	273, 864, 980, 1015, 1078, 1617, 2116
Bst6I	CTCTTC	3 cut(s)	338, 1251, 1480
BstACI	GRCGYC	1 cut(s)	740
BstAUI	TGTACA	1 cut(s)	762
BstDEI	CTNAG	6 cut(s)	72, 365, 1230, 1418, 1621, 1752
BstENI	CCTNNNNNAGG	1 cut(s)	2035
BstF5I	GGATG	4 cut(s)	533, 1704, 2065, 2228
BstH2I	RGCGCY	3 cut(s)	743, 975, 1181
BstHHI	GCGC	6 cut(s)	506, 698, 742, 974, 1126, 1180
BstMAI	GTCTC	1 cut(s)	870
BstMWI	GCNNNNNNNGC	8 cut(s)	302, 746, 755, 969, 1364, 1391, 1556, 1658
BstNI	CCWGG	5 cut(s)	253, 830, 1003, 1533, 1845
BstNSI	RCATGY	2 cut(s)	510, 1579
BstSCI	CCNGG	8 cut(s)	251, 312, 828, 1001, 1491, 1531, 1739, 1843
BstSFI	CTRYAG	6 cut(s)	675, 1359, 1551, 1868, 2074, 2259
BstSLI	GKGCMC	1 cut(s)	1431
BstV1I	GCAGC	8 cut(s)	602, 661, 812, 943, 1537, 1673, 1712, 1965
BstV2I	GAAGAC	2 cut(s)	1329, 1775
BstX2I	RGATCY	2 cut(s)	1300, 1864
BstXI	CCANNNNNNTGG	1 cut(s)	639
BstYI	RGATCY	2 cut(s)	1300, 1864
BsuRI	GGCC	6 cut(s)	405, 445, 1022, 1394, 1896, 1994
BtgZI	GCGATG	1 cut(s)	2205
BtrI	CACGTC	1 cut(s)	1543
BtsCI	GGATG	4 cut(s)	533, 1704, 2065, 2228
BtsI	GCAGTG	1 cut(s)	1044
BtsIMutI	CAGTG	6 cut(s)	253, 860, 1044, 1052, 1415, 2139
BveI	ACCTGC	2 cut(s)	668, 1262
CciI	TCATGA	2 cut(s)	1293, 1719
CfoI	GCGC	6 cut(s)	506, 698, 742, 974, 1126, 1180
Cfr10I	RCCGGY	1 cut(s)	1022
Cfr13I	GGNCC	5 cut(s)	403, 769, 826, 1020, 1484
Csp6I	GTAC	9 cut(s)	217, 223, 689, 763, 1132, 1262, 1443, 1618, 2127
CspCI	CAANNNNNGTGG	2 cut(s)	717, 752
CviQI	GTAC	9 cut(s)	217, 223, 689, 763, 1132, 1262, 1443, 1618, 2127
DdeI	CTNAG	6 cut(s)	72, 365, 1230, 1418, 1621, 1752
DinI	GGCGCC	1 cut(s)	741
EaeI	YGGCCR	2 cut(s)	443, 1992
Eam1104I	CTCTTC	3 cut(s)	338, 1251, 1480
EarI	CTCTTC	3 cut(s)	338, 1251, 1480
Eco130I	CCWWGG	2 cut(s)	632, 1897
Eco24I	GRGCYC	2 cut(s)	565, 751
Eco47I	GGWCC	3 cut(s)	769, 826, 1484
Eco57I	CTGAAG	4 cut(s)	229, 501, 556, 2017
Eco88I	CYCGRG	1 cut(s)	1404
EcoNI	CCTNNNNNAGG	1 cut(s)	2035
EcoO109I	RGGNCCY	1 cut(s)	769
EcoRII	CCWGG	5 cut(s)	251, 828, 1001, 1531, 1843
EcoT14I	CCWWGG	2 cut(s)	632, 1897
EcoT22I	ATGCAT	2 cut(s)	240, 1577
EcoT38I	GRGCYC	2 cut(s)	565, 751
EgeI	GGCGCC	1 cut(s)	741
EheI	GGCGCC	1 cut(s)	741
ErhI	CCWWGG	2 cut(s)	632, 1897
FaqI	GGGAC	3 cut(s)	650, 865, 1827
FauI	CCCGC	2 cut(s)	1795, 1804
FbaI	TGATCA	1 cut(s)	414
FblI	GTMKAC	2 cut(s)	155, 1539
Fnu4HI	GCNGC	9 cut(s)	591, 675, 801, 957, 1551, 1638, 1662, 1701, 1954
FokI	GGATG	4 cut(s)	520, 1691, 2072, 2235
FriOI	GRGCYC	2 cut(s)	565, 751
Fsp4HI	GCNGC	9 cut(s)	591, 675, 801, 957, 1551, 1638, 1662, 1701, 1954
FspBI	CTAG	5 cut(s)	359, 804, 2124, 2132, 2278
GlaI	GCGC	6 cut(s)	505, 697, 741, 973, 1125, 1179
GluI	GCNGC	9 cut(s)	591, 675, 801, 957, 1551, 1638, 1662, 1701, 1954
GsuI	CTGGAG	1 cut(s)	1515
HaeII	RGCGCY	3 cut(s)	743, 975, 1181
HaeIII	GGCC	6 cut(s)	405, 445, 1022, 1394, 1896, 1994
HapII	CCGG	9 cut(s)	293, 314, 435, 1023, 1284, 1457, 1493, 1653, 1740
HhaI	GCGC	6 cut(s)	506, 698, 742, 974, 1126, 1180
Hin1I	GRCGYC	1 cut(s)	740
Hin6I	GCGC	6 cut(s)	504, 696, 740, 972, 1124, 1178
HinP1I	GCGC	6 cut(s)	504, 696, 740, 972, 1124, 1178
HincII	GTYRAC	2 cut(s)	669, 1540
HindII	GTYRAC	2 cut(s)	669, 1540
HindIII	AAGCTT	1 cut(s)	551
HpaII	CCGG	9 cut(s)	293, 314, 435, 1023, 1284, 1457, 1493, 1653, 1740
HphI	GGTGA	4 cut(s)	621, 1446, 1673, 2215
Hpy166II	GTNNAC	8 cut(s)	36, 156, 669, 1111, 1429, 1540, 1585, 1681
Hpy188III	TCNNGA	7 cut(s)	188, 197, 1061, 1294, 1479, 1623, 1720
Hpy8I	GTNNAC	8 cut(s)	36, 156, 669, 1111, 1429, 1540, 1585, 1681
Hpy99I	CGWCG	2 cut(s)	971, 1544
HpyCH4III	ACNGT	7 cut(s)	273, 864, 980, 1015, 1078, 1617, 2116
HpyCH4IV	ACGT	3 cut(s)	1521, 1542, 2052
HpyF10VI	GCNNNNNNNGC	8 cut(s)	302, 746, 755, 969, 1364, 1391, 1556, 1658
HpyF3I	CTNAG	6 cut(s)	72, 365, 1230, 1418, 1621, 1752
HpySE526I	ACGT	3 cut(s)	1521, 1542, 2052
Hsp92I	GRCGYC	1 cut(s)	740
HspAI	GCGC	6 cut(s)	504, 696, 740, 972, 1124, 1178
KasI	GGCGCC	1 cut(s)	739
KroI	GCCGGC	1 cut(s)	1022
KroNI	GCCGGC	1 cut(s)	1024
Ksp22I	TGATCA	1 cut(s)	414
LguI	GCTCTTC	1 cut(s)	338
LmnI	GCTCC	3 cut(s)	353, 995, 1534
Lsp1109I	GCAGC	8 cut(s)	602, 661, 812, 943, 1537, 1673, 1712, 1965
LweI	GCATC	2 cut(s)	1111, 2182
MaeI	CTAG	5 cut(s)	359, 804, 2124, 2132, 2278
MaeII	ACGT	3 cut(s)	1521, 1542, 2052
MaeIII	GTNAC	6 cut(s)	464, 680, 858, 1495, 1600, 2044
MboII	GAAGA	8 cut(s)	171, 203, 325, 1268, 1282, 1334, 1467, 1775
MflI	RGATCY	2 cut(s)	1300, 1864
MhlI	GDGCHC	4 cut(s)	565, 751, 1000, 1431
MlsI	TGGCCA	2 cut(s)	445, 1994
MluCI	AATT	7 cut(s)	163, 278, 834, 939, 1168, 1588, 1747
MluNI	TGGCCA	2 cut(s)	445, 1994
Mly113I	GGCGCC	1 cut(s)	740
MlyI	GAGTC	8 cut(s)	326, 779, 887, 1051, 1545, 1702, 1842, 2006
Mox20I	TGGCCA	2 cut(s)	445, 1994
Mph1103I	ATGCAT	2 cut(s)	240, 1577
MroNI	GCCGGC	1 cut(s)	1022
MscI	TGGCCA	2 cut(s)	445, 1994
MseI	TTAA	4 cut(s)	161, 170, 1410, 2001
MslI	CAYNNNNRTG	1 cut(s)	98
Msp20I	TGGCCA	2 cut(s)	445, 1994
MspA1I	CMGCKG	2 cut(s)	674, 1967
MspI	CCGG	9 cut(s)	293, 314, 435, 1023, 1284, 1457, 1493, 1653, 1740
MspR9I	CCNGG	8 cut(s)	253, 314, 830, 1003, 1493, 1533, 1741, 1845
MvaI	CCWGG	5 cut(s)	253, 830, 1003, 1533, 1845
MwoI	GCNNNNNNNGC	8 cut(s)	302, 746, 755, 969, 1364, 1391, 1556, 1658
NaeI	GCCGGC	1 cut(s)	1024
NarI	GGCGCC	1 cut(s)	740
NciI	CCSGG	3 cut(s)	314, 1493, 1741
NgoMIV	GCCGGC	1 cut(s)	1022
NlaIV	GGNNCC	5 cut(s)	741, 1021, 1366, 1641, 2094
NmuCI	GTSAC	4 cut(s)	464, 858, 1495, 2044
NsiI	ATGCAT	2 cut(s)	240, 1577
NspI	RCATGY	2 cut(s)	510, 1579
PaeI	GCATGC	2 cut(s)	510, 1579
PaeR7I	CTCGAG	1 cut(s)	1404
PagI	TCATGA	2 cut(s)	1293, 1719
PaqCI	CACCTGC	1 cut(s)	1262
PciSI	GCTCTTC	1 cut(s)	338
PdiI	GCCGGC	1 cut(s)	1024
PfeI	GAWTC	3 cut(s)	48, 626, 1287
PflFI	GACNNNGTC	1 cut(s)	1493
PfoI	TCCNGGA	1 cut(s)	1531
PkrI	GCNGC	9 cut(s)	592, 676, 802, 958, 1552, 1639, 1663, 1702, 1955
PleI	GAGTC	8 cut(s)	325, 779, 886, 1051, 1544, 1701, 1842, 2006
PluTI	GGCGCC	1 cut(s)	743
PpsI	GAGTC	8 cut(s)	325, 779, 886, 1051, 1544, 1701, 1842, 2006
PpuMI	RGGWCCY	1 cut(s)	769
Psp5II	RGGWCCY	1 cut(s)	769
Psp6I	CCWGG	5 cut(s)	251, 828, 1001, 1531, 1843
PspGI	CCWGG	5 cut(s)	251, 828, 1001, 1531, 1843
PspN4I	GGNNCC	5 cut(s)	741, 1021, 1366, 1641, 2094
PspPI	GGNCC	5 cut(s)	403, 769, 826, 1020, 1484
PspPPI	RGGWCCY	1 cut(s)	769
PspXI	VCTCGAGB	1 cut(s)	1404
PstI	CTGCAG	3 cut(s)	679, 1555, 1872
PsuI	RGATCY	2 cut(s)	1300, 1864
PsyI	GACNNNGTC	1 cut(s)	1493
PvuII	CAGCTG	2 cut(s)	674, 1967
RsaI	GTAC	9 cut(s)	218, 224, 690, 764, 1133, 1263, 1444, 1619, 2128
RsaNI	GTAC	9 cut(s)	217, 223, 689, 763, 1132, 1262, 1443, 1618, 2127
RseI	CAYNNNNRTG	1 cut(s)	98
SalI	GTCGAC	1 cut(s)	1538
SapI	GCTCTTC	1 cut(s)	338
SaqAI	TTAA	4 cut(s)	161, 170, 1410, 2001
SatI	GCNGC	9 cut(s)	591, 675, 801, 957, 1551, 1638, 1662, 1701, 1954
Sau96I	GGNCC	5 cut(s)	403, 769, 826, 1020, 1484
ScaI	AGTACT	1 cut(s)	2128
SchI	GAGTC	8 cut(s)	326, 779, 887, 1051, 1545, 1702, 1842, 2006
ScrFI	CCNGG	8 cut(s)	253, 314, 830, 1003, 1493, 1533, 1741, 1845
SduI	GDGCHC	4 cut(s)	565, 751, 1000, 1431
SfaNI	GCATC	2 cut(s)	1111, 2182
SfcI	CTRYAG	6 cut(s)	675, 1359, 1551, 1868, 2074, 2259
SfoI	GGCGCC	1 cut(s)	741
Sfr274I	CTCGAG	1 cut(s)	1404
SinI	GGWCC	3 cut(s)	769, 826, 1484
SlaI	CTCGAG	1 cut(s)	1404
SmiMI	CAYNNNNRTG	1 cut(s)	98
SmlI	CTYRAG	2 cut(s)	1404, 2170
SmoI	CTYRAG	2 cut(s)	1404, 2170
SphI	GCATGC	2 cut(s)	510, 1579
Sse9I	AATT	7 cut(s)	163, 278, 834, 939, 1168, 1588, 1747
SsiI	CCGC	3 cut(s)	1637, 1788, 1811
SspDI	GGCGCC	1 cut(s)	739
SspMI	CTAG	5 cut(s)	359, 804, 2124, 2132, 2278
StyD4I	CCNGG	8 cut(s)	251, 312, 828, 1001, 1491, 1531, 1739, 1843
StyI	CCWWGG	2 cut(s)	632, 1897
TaaI	ACNGT	7 cut(s)	273, 864, 980, 1015, 1078, 1617, 2116
TaiI	ACGT	3 cut(s)	1524, 1545, 2055
TaqI	TCGA	4 cut(s)	565, 1405, 1539, 2184
TasI	AATT	7 cut(s)	163, 278, 834, 939, 1168, 1588, 1747
TatI	WGTACW	6 cut(s)	216, 688, 762, 1131, 1617, 2126
TauI	GCSGC	1 cut(s)	1640
TfiI	GAWTC	3 cut(s)	48, 626, 1287
Tru1I	TTAA	4 cut(s)	161, 170, 1410, 2001
Tru9I	TTAA	4 cut(s)	161, 170, 1410, 2001
TscAI	CASTG	6 cut(s)	253, 867, 1051, 1059, 1422, 2146
TseFI	GTSAC	4 cut(s)	464, 858, 1495, 2044
TseI	GCWGC	8 cut(s)	590, 674, 800, 956, 1550, 1661, 1700, 1953
Tsp45I	GTSAC	4 cut(s)	464, 858, 1495, 2044
TspDTI	ATGAA	9 cut(s)	153, 171, 219, 1282, 1626, 1853, 1934, 1978, 2076
TspGWI	ACGGA	2 cut(s)	536, 2019
TspRI	CASTG	6 cut(s)	253, 867, 1051, 1059, 1422, 2146
Tth111I	GACNNNGTC	1 cut(s)	1493
VneI	GTGCAC	1 cut(s)	1427
VpaK11BI	GGWCC	3 cut(s)	769, 826, 1484
XagI	CCTNNNNNAGG	1 cut(s)	2035
XapI	RAATTY	2 cut(s)	163, 1747
XceI	RCATGY	2 cut(s)	510, 1579
XcmI	CCANNNNNNNNNTGG	1 cut(s)	236
XhoI	CTCGAG	1 cut(s)	1404
XmiI	GTMKAC	2 cut(s)	155, 1539
XspI	CTAG	5 cut(s)	359, 804, 2124, 2132, 2278
ZrmI	AGTACT	1 cut(s)	2128
Zsp2I	ATGCAT	2 cut(s)	240, 1577

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.