Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002861

Physical Location & Seq

Genomic Coordinates Reverse (-)

5471545 .. 5473830

Gene Structure 2286 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1080300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGATTTGTCCAGCATGACCACACTTCTGTTGTTAATGCTGTTGCAGATCAACAATGCAGTATCGAATGTTGAGGAAATCCAGACGTACATCATTCACATGGATCATTCCCACAAACCCGAATCGTTCTTAACGCACGAAGCGTGGCACAAGTCCACCGTAAAGTCATTGTCGTCTTCGTCTCCTGCAGAAAACGGTGATGGCAGTGAAATGTTGCTGTACTCATACAGCCATGTCATGCATGGGTTCAGTGCAAGGCTCACTCCTTCTCAGTTGTCTAGATTGGAGAAATCCCCGGCTCATGTTGCCACGTATCCCGAGTCTTTTGGCAAGATGTTCACAACTCACAGCCCTAAGTTTCTTGGACTTAGGCAGAACCTCGGTCTATGGCCTAATGCCTCATTTGGCCAAGATGTGATCATAGGAATTATTGATACAGGAATTTGGCCAGAAAGTGAGAGTTTCAGCGATAAGGGAATGTCAGAGGTGCCATTAAGATGGAAAGGCGTGTGCGAGAATGGAACTGCATTCACCACGTCTCTTTGCAACAAGAAGCTCATTGGTGCTCGATCTTTCAGCAAAGGGCTCGAAGCTGCAGGCATACCGATTTCCAGACAATATGACTATGATTCGCCGAGAGACTTCTTCGGTCACGGAACACACACATCATCGACAGCTGCAGGCAACCATGTGCTCAGTGCAAGTCATTTCGGATATGCAAAGGGCACGGCAAGAGGGGTGGCGCCACGCGCCCACGTTGCCATGTACAAGGTCTTGTGGGCGACAGATTCAGAAGAGAGTGCAGCTAGTGATGTGCTTGCCGGGATGGATCAGGCGATTTCTGATGGTGTTGATATCATGTCACTGTCTCTTGGCTTCGACGTCTCTCCTTACTACAATGATATAATTGCCATCAGTTCTCTTTCAGCAGTTGAGAAGGGAATTGCTGTTGTATGTGCTGCTGGGAATGATGGTCCTTCTGATAACTCAACACATAATGGAGCACCCTGGATCACAACAGTAGGAGCTGGCACACTTGATCGTACTTTCACTGCAACATTGACTCTAGACAACGGCTACACAGTTGAAGGAGAATCATACTTCCCCGAAAGCGTTTACATTGCTGATAAACCGTTATACTACGGGAAAGACAGCGTTAGCAAATCAACGTGCAACTTTGGGGCATTAGATCACAAAGAAGTGGCTGGAAAAGTAGTCTTCTGTGATAATCCCCAGGGGGGTGACATTGATGGACAACTTGAAGAGGTAAAAAACGCAGGCGCTTACGCTGCAATCTTTGTGTCAGATATGCCAGATTTGTACGCTGAAGACTTCACCATTCCTGCTTTGGTTCTACCAATGGCGAAAGGCTCTTTAATTAAGGAGTATGCAACAAGAGCCAATACAACTAAAGTTAAAACCCTTACCTTTTTGCACACCAATTTGGGGACAAAACCGGCACCGGAAGTAGCGTCTTTCTCTTCAAGAGGACCTGACCCCATCACCCCAAGCATTCTAAAACCCGACATTCTTGCTCCCGGTGTTGATGTACTCGCTGCAGTTGCACCTAACAAGATGTTGATGGAAGTAAACAACTATAATTTGGCTTCAGACTATCAACTAATGTCAGGGACATCAATGGCAGCACCCCATGTTGCAGGAGTTGTAGCTCTCCTAAAGGCTGTTCACCGCGAATGGAGCCCTGCTGCCATTCGATCAGCGATCATGACCACAGCGTATAACCTCGACAACACTAGAACCACCATAAAAGACCAACGAGACGGTCTTGCTGCCACACCTTTACAATTCGGTGCAGGCCATATCAACCCGAACAGAGCCATGAATCCTGGACTCGTCTATGACATGGATGTGCAGGATTATATCGAGTTTCTATGTGGCCTCGGATACACTACCAAGCAGATGAGCGCTGTGATCCGACGAAATCAATGGAGTTGCAGGCAACAACCTACTGAGCTAAACTACCCTTCTTTTATAGCCATCTTCAACAGCACAGACAACTCTCCGAAAGCAAAAAACTTTACCAGGGTTGTGACAAATGTGGGAGATGATGCATCAAGTTACTTTGCATTCTTGGAGGTTCCTAAAGGAATGAAGATCGCAGTGGAGCCTAGCACTCTAACATTCACGGGACAGAATCAAGAACAAGGTTTCGTGTTGAGAGTAGAGATCGATAGCAACGCTCCGAAGGTGACGTATGGGTATCTGAAATGGATTGATCAACATAACCACATAGTATCAAGCCCGGTAGTGGTCGTAAATTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

762

Amino Acids

82.46

Weight (kDa)

5.59

Isoelectric Point (pI)

40.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	29 - 108	6.4e-16	Peptidase inhibitor I9
Peptidase_S8	PF00082	136 - 590	2.7e-48	Subtilase family
fn3_6	PF17766	658 - 758	2.8e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002861)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34120 FvH4_2g34130 FvH4_2g34150 FvH4_2g34210 FvH4_2g34211 FvH4_2g34212
malus_domestica	MD05G1275700.v1.1 MD05G1275800.v1.1 MD08G1098000.v1.1 MD15G1080200.v1.1 MD15G1080300.v1.1
prunus_persica	Prupe.1G433100_v2.0.a1 Prupe.1G433200_v2.0.a1
pyrus_communis	pycom15g07460
rosa_chinensis	RchiOBHm_Chr6g0308201 RchiOBHm_Chr6g0308211 RchiOBHm_Chr6g0308221
rosa_laevigata	RLG00000010657 RLG00000010658 RLG00000010659
rosa_multiflora	Rmu_sc0005706.1_g000017 Rmu_sc0005706.1_g000018 Rmu_ssc0000355.1_g000001 Rmu_ssc0000355.1_g000006
rosa_roxburghii	Rroxscaffold_7G00160340 Rroxscaffold_7G00160360 Rroxscaffold_7G00160440 Rroxscaffold_7G00160450
rosa_rugosa	Rorug01G0005200 Rorug01G0066300 Rorug06G0364700 Rorug06G0364800 Rorug06G0364900
rosa_samantha	Rh6DG475900 Rh6DG476100 Rh6DG476300
rosa_wichuraiana	Rw6G041330 Rw6G041340 Rw6G041350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	885
AccB1I	GGYRCC	3 cut(s)	487, 742, 1459
AccII	CGCG	2 cut(s)	750, 1692
AciI	CCGC	1 cut(s)	1690
AclWI	GGATC	4 cut(s)	111, 837, 1019, 1927
AcoI	YGGCCR	2 cut(s)	406, 446
AcsI	RAATTY	1 cut(s)	441
AcuI	CTGAAG	2 cut(s)	1347, 1593
AcyI	GRCGYC	2 cut(s)	743, 882
AfaI	GTAC	6 cut(s)	89, 221, 767, 1045, 1322, 1551
AfeI	AGCGCT	1 cut(s)	1927
AfiI	CCNNNNNNNGG	3 cut(s)	1238, 1446, 2269
AgsI	TTSAA	4 cut(s)	1088, 1262, 1485, 2005
AjiI	CACGTC	1 cut(s)	537
AjnI	CCWGG	4 cut(s)	1007, 1233, 1846, 2042
AluBI	AGCT	7 cut(s)	556, 593, 677, 806, 1028, 1670, 1975
AluI	AGCT	7 cut(s)	556, 593, 677, 806, 1028, 1670, 1975
Alw21I	GWGCWC	3 cut(s)	568, 696, 1006
Alw26I	GTCTC	6 cut(s)	186, 543, 633, 873, 889, 1773
AlwI	GGATC	4 cut(s)	111, 837, 1019, 1927
Ama87I	CYCGRG	1 cut(s)	317
Aor51HI	AGCGCT	1 cut(s)	1927
AoxI	GGCC	5 cut(s)	389, 406, 446, 1816, 1897
ApeKI	GCWGC	9 cut(s)	593, 677, 803, 959, 1289, 1556, 1643, 1706, 1790
ApoI	RAATTY	1 cut(s)	441
AspLEI	GCGC	4 cut(s)	745, 752, 1283, 1928
AspS9I	GGNCC	2 cut(s)	974, 1490
AsuC2I	CCSGG	4 cut(s)	296, 823, 1539, 2264
AsuHPI	GGTGA	7 cut(s)	209, 523, 1253, 1327, 1495, 1679, 2221
AvaI	CYCGRG	1 cut(s)	317
AvaII	GGWCC	2 cut(s)	974, 1490
BaeGI	GKGCMC	1 cut(s)	728
BalI	TGGCCA	2 cut(s)	408, 448
BanI	GGYRCC	3 cut(s)	487, 742, 1459
BanII	GRGCYC	2 cut(s)	588, 1703
BbsI	GAAGAC	3 cut(s)	168, 1210, 1335
Bbv12I	GWGCWC	3 cut(s)	568, 696, 1006
BbvI	GCAGC	9 cut(s)	580, 664, 815, 946, 1276, 1543, 1655, 1693, 1777
BceAI	ACGGC	2 cut(s)	744, 1090
BcgI	CGANNNNNNTGC	4 cut(s)	568, 602, 1514, 1548
BciT130I	CCWGG	4 cut(s)	1009, 1235, 1848, 2044
BciVI	GTATCC	2 cut(s)	324, 1898
BclI	TGATCA	2 cut(s)	417, 2236
BcnI	CCSGG	4 cut(s)	296, 823, 1539, 2264
BcoDI	GTCTC	6 cut(s)	186, 543, 633, 873, 889, 1773
BfaI	CTAG	6 cut(s)	279, 807, 1067, 1755, 2130, 2284
BfmI	CTRYAG	4 cut(s)	186, 594, 678, 1557
BfoI	RGCGCY	3 cut(s)	746, 1284, 1929
BfuI	GTATCC	2 cut(s)	324, 1898
BisI	GCNGC	9 cut(s)	594, 678, 804, 960, 1290, 1557, 1644, 1707, 1791
BlsI	GCNGC	9 cut(s)	595, 679, 805, 961, 1291, 1558, 1645, 1708, 1792
Bme1390I	CCNGG	8 cut(s)	296, 823, 1009, 1235, 1539, 1848, 2044, 2264
Bme18I	GGWCC	2 cut(s)	974, 1490
BmeT110I	CYCGRG	1 cut(s)	317
BmgBI	CACGTC	1 cut(s)	537
BmgT120I	GGNCC	2 cut(s)	974, 1490
BmiI	GGNNCC	6 cut(s)	489, 744, 1461, 1700, 2100, 2127
BmrFI	CCNGG	8 cut(s)	296, 823, 1009, 1235, 1539, 1848, 2044, 2264
BmsI	GCATC	2 cut(s)	2059, 2081
BpiI	GAAGAC	3 cut(s)	168, 1210, 1335
BpuMI	CCSGG	4 cut(s)	296, 823, 1539, 2264
Bsa29I	ATCGAT	1 cut(s)	2190
BsaAI	YACGTR	1 cut(s)	312
BsaHI	GRCGYC	2 cut(s)	743, 882
BsaJI	CCNNGG	7 cut(s)	294, 379, 1007, 1233, 1234, 1900, 2043
BsaWI	WCCGGW	1 cut(s)	1462
Bsc4I	CCNNNNNNNGG	3 cut(s)	1238, 1446, 2269
Bse118I	RCCGGY	1 cut(s)	1456
Bse3DI	GCAATG	1 cut(s)	1119
BseBI	CCWGG	4 cut(s)	1009, 1235, 1848, 2044
BseCI	ATCGAT	1 cut(s)	2190
BseDI	CCNNGG	7 cut(s)	294, 379, 1007, 1233, 1234, 1900, 2043
BseGI	GGATG	2 cut(s)	831, 1873
BseLI	CCNNNNNNNGG	3 cut(s)	1238, 1446, 2269
BseMI	GCAATG	1 cut(s)	1119
BseMII	CTCAG	3 cut(s)	284, 709, 1962
BseSI	GKGCMC	1 cut(s)	728
BseXI	GCAGC	9 cut(s)	580, 664, 815, 946, 1276, 1543, 1655, 1693, 1777
BseYI	CCCAGC	1 cut(s)	962
BsgI	GTGCAG	3 cut(s)	822, 1833, 1892
Bsh1236I	CGCG	2 cut(s)	750, 1692
BshFI	GGCC	5 cut(s)	391, 408, 448, 1818, 1899
BshNI	GGYRCC	3 cut(s)	487, 742, 1459
BshVI	ATCGAT	1 cut(s)	2190
BsiHKAI	GWGCWC	3 cut(s)	568, 696, 1006
BsiHKCI	CYCGRG	1 cut(s)	317
BsiSI	CCGG	6 cut(s)	296, 822, 1457, 1463, 1539, 2264
BslFI	GGGAC	3 cut(s)	1462, 1645, 2163
BslI	CCNNNNNNNGG	3 cut(s)	1238, 1446, 2269
BsmAI	GTCTC	6 cut(s)	186, 543, 633, 873, 889, 1773
BsmBI	CGTCTC	4 cut(s)	186, 543, 889, 1773
BsmFI	GGGAC	3 cut(s)	1462, 1645, 2163
BsmI	GAATGC	3 cut(s)	527, 1512, 2087
BsnI	GGCC	5 cut(s)	391, 408, 448, 1818, 1899
BsoBI	CYCGRG	1 cut(s)	317
Bsp1286I	GDGCHC	6 cut(s)	568, 588, 696, 728, 1006, 1703
Bsp1407I	TGTACA	1 cut(s)	765
BspACI	CCGC	1 cut(s)	1690
BspANI	GGCC	5 cut(s)	391, 408, 448, 1818, 1899
BspCNI	CTCAG	3 cut(s)	283, 708, 1963
BspDI	ATCGAT	1 cut(s)	2190
BspFNI	CGCG	2 cut(s)	750, 1692
BspHI	TCATGA	1 cut(s)	1725
BspLI	GGNNCC	6 cut(s)	489, 744, 1461, 1700, 2100, 2127
BspMAI	CTGCAG	4 cut(s)	190, 598, 682, 1561
BspPI	GGATC	4 cut(s)	111, 837, 1019, 1927
BspT107I	GGYRCC	3 cut(s)	487, 742, 1459
BsrDI	GCAATG	1 cut(s)	1119
BsrFI	RCCGGY	1 cut(s)	1456
BsrGI	TGTACA	1 cut(s)	765
BssAI	RCCGGY	1 cut(s)	1456
BssECI	CCNNGG	7 cut(s)	294, 379, 1007, 1233, 1234, 1900, 2043
BssNI	GRCGYC	2 cut(s)	743, 882
Bst2UI	CCWGG	4 cut(s)	1009, 1235, 1848, 2044
Bst4CI	ACNGT	7 cut(s)	160, 197, 867, 1021, 1084, 1134, 1784
Bst6I	CTCTTC	3 cut(s)	789, 1257, 1486
BstACI	GRCGYC	2 cut(s)	743, 882
BstAUI	TGTACA	1 cut(s)	765
BstBAI	YACGTR	1 cut(s)	312
BstC8I	GCNNGC	7 cut(s)	598, 682, 819, 1030, 1279, 1816, 1958
BstDEI	CTNAG	5 cut(s)	270, 354, 368, 695, 1971
BstF5I	GGATG	2 cut(s)	831, 1873
BstFNI	CGCG	2 cut(s)	750, 1692
BstH2I	RGCGCY	3 cut(s)	746, 1284, 1929
BstHHI	GCGC	4 cut(s)	745, 752, 1283, 1928
BstMAI	GTCTC	6 cut(s)	186, 543, 633, 873, 889, 1773
BstMWI	GCNNNNNNNGC	7 cut(s)	305, 749, 758, 1289, 1397, 1562, 1698
BstNI	CCWGG	4 cut(s)	1009, 1235, 1848, 2044
BstSCI	CCNGG	8 cut(s)	294, 821, 1007, 1233, 1537, 1846, 2042, 2262
BstSFI	CTRYAG	4 cut(s)	186, 594, 678, 1557
BstSLI	GKGCMC	1 cut(s)	728
BstUI	CGCG	2 cut(s)	750, 1692
BstV1I	GCAGC	9 cut(s)	580, 664, 815, 946, 1276, 1543, 1655, 1693, 1777
BstV2I	GAAGAC	3 cut(s)	168, 1210, 1335
BstXI	CCANNNNNNTGG	1 cut(s)	498
Bsu15I	ATCGAT	1 cut(s)	2190
BsuI	GTATCC	2 cut(s)	324, 1898
BsuRI	GGCC	5 cut(s)	391, 408, 448, 1818, 1899
BsuTUI	ATCGAT	1 cut(s)	2190
BtrI	CACGTC	1 cut(s)	537
BtsCI	GGATG	2 cut(s)	831, 1873
BtsI	GCAGTG	3 cut(s)	211, 1050, 2127
BtsIMutI	CAGTG	6 cut(s)	211, 256, 703, 863, 1050, 2127
Cac8I	GCNNGC	7 cut(s)	598, 682, 819, 1030, 1279, 1816, 1958
CciI	TCATGA	1 cut(s)	1725
CfoI	GCGC	4 cut(s)	745, 752, 1283, 1928
Cfr10I	RCCGGY	1 cut(s)	1456
Cfr13I	GGNCC	2 cut(s)	974, 1490
ClaI	ATCGAT	1 cut(s)	2190
CseI	GACGC	1 cut(s)	1461
Csp6I	GTAC	6 cut(s)	88, 220, 766, 1044, 1321, 1550
CspCI	CAANNNNNGTGG	2 cut(s)	720, 755
CviQI	GTAC	6 cut(s)	88, 220, 766, 1044, 1321, 1550
DdeI	CTNAG	5 cut(s)	270, 354, 368, 695, 1971
DinI	GGCGCC	1 cut(s)	744
EaeI	YGGCCR	2 cut(s)	406, 446
Eam1104I	CTCTTC	3 cut(s)	789, 1257, 1486
EarI	CTCTTC	3 cut(s)	789, 1257, 1486
Eco24I	GRGCYC	2 cut(s)	588, 1703
Eco32I	GATATC	1 cut(s)	856
Eco47I	GGWCC	2 cut(s)	974, 1490
Eco47III	AGCGCT	1 cut(s)	1927
Eco57I	CTGAAG	2 cut(s)	1347, 1593
Eco88I	CYCGRG	1 cut(s)	317
EcoO109I	RGGNCCY	1 cut(s)	1490
EcoRII	CCWGG	4 cut(s)	1007, 1233, 1846, 2042
EcoRV	GATATC	1 cut(s)	856
EcoT22I	ATGCAT	2 cut(s)	243, 2074
EcoT38I	GRGCYC	2 cut(s)	588, 1703
EgeI	GGCGCC	1 cut(s)	744
EheI	GGCGCC	1 cut(s)	744
Esp3I	CGTCTC	4 cut(s)	186, 543, 889, 1773
FaqI	GGGAC	3 cut(s)	1462, 1645, 2163
FbaI	TGATCA	2 cut(s)	417, 2236
Fnu4HI	GCNGC	9 cut(s)	594, 678, 804, 960, 1290, 1557, 1644, 1707, 1791
FokI	GGATG	2 cut(s)	838, 1880
FriOI	GRGCYC	2 cut(s)	588, 1703
Fsp4HI	GCNGC	9 cut(s)	594, 678, 804, 960, 1290, 1557, 1644, 1707, 1791
FspBI	CTAG	6 cut(s)	279, 807, 1067, 1755, 2130, 2284
GlaI	GCGC	4 cut(s)	744, 751, 1282, 1927
GluI	GCNGC	9 cut(s)	594, 678, 804, 960, 1290, 1557, 1644, 1707, 1791
GsaI	CCCAGC	1 cut(s)	966
HaeII	RGCGCY	3 cut(s)	746, 1284, 1929
HaeIII	GGCC	5 cut(s)	391, 408, 448, 1818, 1899
HapII	CCGG	6 cut(s)	296, 822, 1457, 1463, 1539, 2264
HgaI	GACGC	1 cut(s)	1461
HhaI	GCGC	4 cut(s)	745, 752, 1283, 1928
Hin1I	GRCGYC	2 cut(s)	743, 882
Hin6I	GCGC	4 cut(s)	743, 750, 1281, 1926
HinP1I	GCGC	4 cut(s)	743, 750, 1281, 1926
HinfI	GANTC	9 cut(s)	122, 320, 629, 788, 1063, 1094, 1843, 1851, 2155
HpaII	CCGG	6 cut(s)	296, 822, 1457, 1463, 1539, 2264
HphI	GGTGA	7 cut(s)	209, 523, 1253, 1327, 1495, 1679, 2221
Hpy166II	GTNNAC	5 cut(s)	156, 339, 1117, 1591, 1687
Hpy188III	TCNNGA	8 cut(s)	82, 279, 317, 612, 1067, 1485, 1726, 2159
Hpy8I	GTNNAC	5 cut(s)	156, 339, 1117, 1591, 1687
Hpy99I	CGWCG	2 cut(s)	884, 1941
HpyAV	CCTTC	6 cut(s)	276, 931, 987, 1082, 1995, 2200
HpyCH4III	ACNGT	7 cut(s)	160, 197, 867, 1021, 1084, 1134, 1784
HpyCH4IV	ACGT	7 cut(s)	86, 311, 536, 756, 882, 1169, 2213
HpyF10VI	GCNNNNNNNGC	7 cut(s)	305, 749, 758, 1289, 1397, 1562, 1698
HpyF3I	CTNAG	5 cut(s)	270, 354, 368, 695, 1971
HpySE526I	ACGT	7 cut(s)	86, 311, 536, 756, 882, 1169, 2213
Hsp92I	GRCGYC	2 cut(s)	743, 882
HspAI	GCGC	4 cut(s)	743, 750, 1281, 1926
KasI	GGCGCC	1 cut(s)	742
Ksp22I	TGATCA	2 cut(s)	417, 2236
LmnI	GCTCC	6 cut(s)	1001, 1025, 1540, 1698, 2125, 2206
Lsp1109I	GCAGC	9 cut(s)	580, 664, 815, 946, 1276, 1543, 1655, 1693, 1777
LweI	GCATC	2 cut(s)	2059, 2081
MaeI	CTAG	6 cut(s)	279, 807, 1067, 1755, 2130, 2284
MaeII	ACGT	7 cut(s)	86, 311, 536, 756, 882, 1169, 2213
MaeIII	GTNAC	6 cut(s)	650, 861, 1241, 2050, 2078, 2209
MboII	GAAGA	9 cut(s)	168, 637, 806, 1210, 1274, 1340, 1473, 1993, 2125
MhlI	GDGCHC	6 cut(s)	568, 588, 696, 728, 1006, 1703
MlsI	TGGCCA	2 cut(s)	408, 448
MluCI	AATT	9 cut(s)	426, 441, 906, 942, 1377, 1441, 1600, 1805, 2278
MluNI	TGGCCA	2 cut(s)	408, 448
Mly113I	GGCGCC	1 cut(s)	743
MlyI	GAGTC	3 cut(s)	329, 1057, 1845
MmeI	TCCRAC	1 cut(s)	1960
Mox20I	TGGCCA	2 cut(s)	408, 448
Mph1103I	ATGCAT	2 cut(s)	243, 2074
MscI	TGGCCA	2 cut(s)	408, 448
MseI	TTAA	6 cut(s)	35, 131, 494, 1376, 1380, 1416
MslI	CAYNNNNRTG	3 cut(s)	98, 496, 1868
Msp20I	TGGCCA	2 cut(s)	408, 448
MspA1I	CMGCKG	1 cut(s)	677
MspI	CCGG	6 cut(s)	296, 822, 1457, 1463, 1539, 2264
MspR9I	CCNGG	8 cut(s)	296, 823, 1009, 1235, 1539, 1848, 2044, 2264
Mva1269I	GAATGC	3 cut(s)	527, 1512, 2087
MvaI	CCWGG	4 cut(s)	1009, 1235, 1848, 2044
MvnI	CGCG	2 cut(s)	750, 1692
MwoI	GCNNNNNNNGC	7 cut(s)	305, 749, 758, 1289, 1397, 1562, 1698
NarI	GGCGCC	1 cut(s)	743
NciI	CCSGG	4 cut(s)	296, 823, 1539, 2264
NlaIV	GGNNCC	6 cut(s)	489, 744, 1461, 1700, 2100, 2127
NmeAIII	GCCGAG	1 cut(s)	660
NmuCI	GTSAC	5 cut(s)	650, 861, 1241, 2050, 2209
NsiI	ATGCAT	2 cut(s)	243, 2074
PacI	TTAATTAA	1 cut(s)	1380
PagI	TCATGA	1 cut(s)	1725
PasI	CCCWGGG	1 cut(s)	1234
PcsI	WCGNNNNNNNCGW	1 cut(s)	140
PctI	GAATGC	3 cut(s)	527, 1512, 2087
PfeI	GAWTC	6 cut(s)	122, 629, 788, 1094, 1843, 2155
PfoI	TCCNGGA	1 cut(s)	1846
PkrI	GCNGC	9 cut(s)	595, 679, 805, 961, 1291, 1558, 1645, 1708, 1792
PleI	GAGTC	3 cut(s)	328, 1057, 1845
PluTI	GGCGCC	1 cut(s)	746
PpsI	GAGTC	3 cut(s)	328, 1057, 1845
Ppu21I	YACGTR	1 cut(s)	312
PpuMI	RGGWCCY	1 cut(s)	1490
Psp5II	RGGWCCY	1 cut(s)	1490
Psp6I	CCWGG	4 cut(s)	1007, 1233, 1846, 2042
PspFI	CCCAGC	1 cut(s)	962
PspGI	CCWGG	4 cut(s)	1007, 1233, 1846, 2042
PspN4I	GGNNCC	6 cut(s)	489, 744, 1461, 1700, 2100, 2127
PspPI	GGNCC	2 cut(s)	974, 1490
PspPPI	RGGWCCY	1 cut(s)	1490
PstI	CTGCAG	4 cut(s)	190, 598, 682, 1561
PvuII	CAGCTG	1 cut(s)	677
RsaI	GTAC	6 cut(s)	89, 221, 767, 1045, 1322, 1551
RsaNI	GTAC	6 cut(s)	88, 220, 766, 1044, 1321, 1550
RseI	CAYNNNNRTG	3 cut(s)	98, 496, 1868
SaqAI	TTAA	6 cut(s)	35, 131, 494, 1376, 1380, 1416
SatI	GCNGC	9 cut(s)	594, 678, 804, 960, 1290, 1557, 1644, 1707, 1791
Sau96I	GGNCC	2 cut(s)	974, 1490
SchI	GAGTC	3 cut(s)	329, 1057, 1845
ScrFI	CCNGG	8 cut(s)	296, 823, 1009, 1235, 1539, 1848, 2044, 2264
SduI	GDGCHC	6 cut(s)	568, 588, 696, 728, 1006, 1703
SfaNI	GCATC	2 cut(s)	2059, 2081
SfcI	CTRYAG	4 cut(s)	186, 594, 678, 1557
SfoI	GGCGCC	1 cut(s)	744
SinI	GGWCC	2 cut(s)	974, 1490
SmiMI	CAYNNNNRTG	3 cut(s)	98, 496, 1868
Sse9I	AATT	9 cut(s)	426, 441, 906, 942, 1377, 1441, 1600, 1805, 2278
SsiI	CCGC	1 cut(s)	1690
SspDI	GGCGCC	1 cut(s)	742
SspMI	CTAG	6 cut(s)	279, 807, 1067, 1755, 2130, 2284
StyD4I	CCNGG	8 cut(s)	294, 821, 1007, 1233, 1537, 1846, 2042, 2262
TaaI	ACNGT	7 cut(s)	160, 197, 867, 1021, 1084, 1134, 1784
TaiI	ACGT	7 cut(s)	89, 314, 539, 759, 885, 1172, 2216
TaqI	TCGA	9 cut(s)	65, 568, 588, 671, 879, 1714, 1746, 1884, 2190
TaqII	GACCGA	2 cut(s)	371, 638
TasI	AATT	9 cut(s)	426, 441, 906, 942, 1377, 1441, 1600, 1805, 2278
TatI	WGTACW	3 cut(s)	219, 765, 1549
TfiI	GAWTC	6 cut(s)	122, 629, 788, 1094, 1843, 2155
Tru1I	TTAA	6 cut(s)	35, 131, 494, 1376, 1380, 1416
Tru9I	TTAA	6 cut(s)	35, 131, 494, 1376, 1380, 1416
TscAI	CASTG	6 cut(s)	211, 256, 703, 870, 1057, 2127
TseFI	GTSAC	5 cut(s)	650, 861, 1241, 2050, 2209
TseI	GCWGC	9 cut(s)	593, 677, 803, 959, 1289, 1556, 1643, 1706, 1790
Tsp45I	GTSAC	5 cut(s)	650, 861, 1241, 2050, 2209
TspDTI	ATGAA	2 cut(s)	1856, 2126
TspGWI	ACGGA	1 cut(s)	669
TspRI	CASTG	6 cut(s)	211, 256, 703, 870, 1057, 2127
VpaK11BI	GGWCC	2 cut(s)	974, 1490
XapI	RAATTY	1 cut(s)	441
XbaI	TCTAGA	2 cut(s)	278, 1066
XcmI	CCANNNNNNNNNTGG	2 cut(s)	239, 1345
XspI	CTAG	6 cut(s)	279, 807, 1067, 1755, 2130, 2284
ZraI	GACGTC	1 cut(s)	883
Zsp2I	ATGCAT	2 cut(s)	243, 2074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.