Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0002861

Physical Location & Seq

Genomic Coordinates Forward (+)

64187008 .. 64189086

Gene Structure 2079 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG476100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGTTCTTGTACTCATACAACCATGTCATGCACGGTTTCAGTGCCAGGCTTACAGCTTCTCAACTGTCCGAATTGGAGGAGTCTCCGGCTCATGTTGCCACGTATCCGGAGTCTTTTGGTAAGCTCTTGACAACACACAGCTCCAAGTTTCTAGGACTGAGACGGAACTCAGGCTTATTGCTTGCTTCCTCGTCTGGCCAAGATGTGATCATAGGAATCATAGATACCGGAGTTTGGCCAGAGAGCGAGAGTTTCGGTGATAGGGGAATGTCTGAAGTGCCAAAAAGATGGAAAGGCACTTGCGAGAATGGAACATATTTCAGCCCATCTCTCTGCAACAACAAGCTCATTGGGGCTCGATCCTTTAGCAAAGGCCTGAAAGCAGCAGGAATTAAAATCTCCACAAAAAATGACTTCGATTCACCACGGGACTGGTTTGGTCATGGAAGTCACACTTCGTCAACAACTGCAGGTAACCATGTACCCGGCGCAAGTCATTTCGGATATGCAAAAGGCACGGCAAGAGGGGTGGCGCCACGAGCCCATGTTGCCATGTACAAGGTCGCCTGGGCAACAGACTCGGAAAAGAGTTTCGCTAGTGATGTGCTTGCAGGCATGGACCATGCAATTTCTGATGGTGTTGATATAATGTCACTGTCTCTTGGCATTGACTTCCTTCCTTATTTCAACGATGTCATTGCCATTGCTTCTCTTTCTGCAATCGAACAAGGAATTGTTGTGGTCTGTGCTGCTGGAAATGACGGGAAATACAGTACAACATATAATGGAGCACCCTGGATCACAACAGTAGGAGCCGGAACTCTTGATCGCACCTTCACTGCAACACTGACTCTAGATAATGGTTTGACGATTGAAGGAACATCGTACTTCCCCGAGAGCGTTTACATTACTGATGTGCCATTGTACTACGGCAAAGACAATGCAACGAAAGCAATATGCAATACTGGGTCACTGGATCACAACGAAGTGGCAGGAAAGCTAGTCCTTTGTGATAATACCACCGAGACTCCCGTCAATGGAGTCGACTTCCAAAAGAAGGAGGTGGAGAGGGCAGGTGGTTATGCTGGAATCTTCATGGCAGATCTCTCACTTTTAGAGCCAGAAGACTTCTCCATTCCTGCTATCATTGTTCCATTTGCTACAGGGGCCTTGATTAGAGAGTATGCAACGCAGGTCCATACACCTAGAGTTAAGATCCTGAGTTTTGTGCACACAAATTTGGGTACAAAACCATCACCGGAAGTAGCGTATTTCTCTTCAAGAGGACCAGACCCAATCACTCCTGGTATTCTAAAACCAGACATTCTTGCTCCAGGAGTCGATGTGCTTGCTGCAGTTACACCTAACAACCCATGCATGGAAGTAAACAATTATAATCTAGTGACAGATTATGAACTATATTCAGGAACATCAATGGCGGCACCCCATGTCGCTGGTGTGGCAGCTCTGGTAAAGGCTGTTCACCGAAAATGGAGTCCAGCAGCCATCCGATCGGCCATCATGACCACAGCATACTCAACCGCCAATTCTCACACCAGCATACAAGACCAATGGTCCGGTCTTCCAGCCACACCTTTAGATTTTGGTGCAGGCCACATCAACCCGAATAAGGCCATGAACCCCGGACTAGTCTATGACATGAATGTGCAAGACTACATTGATTTTCTTTGTACCCTAGGATACAATGACAAGCAGATGAAAGCTGTTATTAGACGAAGCCAGTGGACTTGCAGACAGCCCCCTACCGAACTAAACTACCCCTCATTCGTGGCCATCTTCAACAAAACAGACTCTCCAAAAGCCAAAAACTTTAGCAGGGTCGTGACAAACGTGGGGGATGATACCTCCATCTACCGAGCATTTCTAGAAGTTCCTAGAGGAATGAGGATTACAGTAGAGCCTAATTCTCTTACATTCACCGAAAAATATCAGCAGCAAGGTTTTGTTTTGAATGTAGAGATCGATAGTGATGCTCCTAAGGTGATCAACGGTTATCTCAGATGGGTTGATCACCATGATCACATGGTATCAAACCCTGTAGTGGCCATTAATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

692

Amino Acids

74.98

Weight (kDa)

5.92

Isoelectric Point (pI)

34.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	3 - 38	7e-06	Peptidase inhibitor I9
Peptidase_S8	PF00082	66 - 517	6.3e-43	Subtilase family
fn3_6	PF17766	590 - 688	5.1e-23	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002861)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34120 FvH4_2g34130 FvH4_2g34150 FvH4_2g34210 FvH4_2g34211 FvH4_2g34212
malus_domestica	MD05G1275700.v1.1 MD05G1275800.v1.1 MD08G1098000.v1.1 MD15G1080200.v1.1 MD15G1080300.v1.1
prunus_persica	Prupe.1G433100_v2.0.a1 Prupe.1G433200_v2.0.a1
pyrus_communis	pycom15g07460
rosa_chinensis	RchiOBHm_Chr6g0308201 RchiOBHm_Chr6g0308211 RchiOBHm_Chr6g0308221
rosa_laevigata	RLG00000010657 RLG00000010658 RLG00000010659
rosa_multiflora	Rmu_sc0005706.1_g000017 Rmu_sc0005706.1_g000018 Rmu_ssc0000355.1_g000001 Rmu_ssc0000355.1_g000006
rosa_roxburghii	Rroxscaffold_7G00160340 Rroxscaffold_7G00160360 Rroxscaffold_7G00160440 Rroxscaffold_7G00160450
rosa_rugosa	Rorug01G0005200 Rorug01G0066300 Rorug06G0364700 Rorug06G0364800 Rorug06G0364900
rosa_samantha	Rh6DG475900 Rh6DG476100 Rh6DG476300
rosa_wichuraiana	Rw6G041330 Rw6G041340 Rw6G041350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1395
AarI	CACCTGC	1 cut(s)	1064
Acc36I	ACCTGC	3 cut(s)	461, 1064, 1183
AccB1I	GGYRCC	2 cut(s)	532, 1441
AccI	GTMKAC	1 cut(s)	1044
AccIII	TCCGGA	1 cut(s)	106
AciI	CCGC	2 cut(s)	1439, 1542
AclWI	GGATC	4 cut(s)	354, 806, 984, 1210
AcoI	YGGCCR	5 cut(s)	196, 236, 1515, 1791, 2064
AcsI	RAATTY	1 cut(s)	1237
AcuI	CTGAAG	1 cut(s)	294
AcyI	GRCGYC	1 cut(s)	533
AfaI	GTAC	8 cut(s)	11, 483, 557, 775, 887, 926, 1246, 1693
AfiI	CCNNNNNNNGG	6 cut(s)	234, 1037, 1057, 1378, 1630, 2062
AgsI	TTSAA	5 cut(s)	688, 875, 1281, 1801, 1972
AhdI	GACNNNNNGTC	1 cut(s)	1573
AhlI	ACTAGT	1 cut(s)	1648
AjnI	CCWGG	5 cut(s)	44, 566, 794, 1303, 1333
AluBI	AGCT	7 cut(s)	56, 124, 141, 346, 1000, 1466, 1724
AluI	AGCT	7 cut(s)	56, 124, 141, 346, 1000, 1466, 1724
Alw21I	GWGCWC	2 cut(s)	793, 1233
Alw26I	GTCTC	4 cut(s)	87, 154, 663, 1019
Alw44I	GTGCAC	1 cut(s)	1229
AlwI	GGATC	4 cut(s)	354, 806, 984, 1210
Ama87I	CYCGRG	1 cut(s)	893
Aor13HI	TCCGGA	1 cut(s)	106
AoxI	GGCC	9 cut(s)	196, 236, 373, 1167, 1515, 1612, 1632, 1791, 2064
ApaLI	GTGCAC	1 cut(s)	1229
ApeKI	GCWGC	6 cut(s)	383, 749, 1352, 1463, 1502, 1954
ApoI	RAATTY	1 cut(s)	1237
ArsI	GACNNNNNNTTYG	2 cut(s)	1480, 1512
AseI	ATTAAT	1 cut(s)	2070
AspA2I	CCTAGG	1 cut(s)	1696
AspLEI	GCGC	2 cut(s)	491, 535
AspS9I	GGNCC	5 cut(s)	619, 1167, 1195, 1286, 1575
AsuC2I	CCSGG	2 cut(s)	486, 1644
AsuHPI	GGTGA	7 cut(s)	269, 414, 1248, 1475, 1930, 2014, 2024
AvaI	CYCGRG	1 cut(s)	893
AvaII	GGWCC	4 cut(s)	619, 1195, 1286, 1575
AvrII	CCTAGG	1 cut(s)	1696
AxyI	CCTNAGG	1 cut(s)	1998
BaeGI	GKGCMC	1 cut(s)	1233
BaeI	ACNNNNGTAYC	2 cut(s)	216, 249
BalI	TGGCCA	4 cut(s)	198, 238, 1793, 2066
BanI	GGYRCC	2 cut(s)	532, 1441
BanII	GRGCYC	2 cut(s)	358, 544
BauI	CACGAG	1 cut(s)	537
BbsI	GAAGAC	2 cut(s)	1131, 1574
Bbv12I	GWGCWC	2 cut(s)	793, 1233
BbvI	GCAGC	6 cut(s)	395, 736, 1339, 1475, 1514, 1966
BccI	CCATC	9 cut(s)	282, 334, 629, 1261, 1514, 1526, 1802, 1877, 2016
BceAI	ACGGC	2 cut(s)	534, 946
BcgI	CGANNNNNNTGC	8 cut(s)	561, 595, 680, 714, 1331, 1365, 1973, 2007
BciT130I	CCWGG	5 cut(s)	46, 568, 796, 1305, 1335
BciVI	GTATCC	2 cut(s)	114, 1694
BclI	TGATCA	4 cut(s)	207, 2004, 2029, 2038
BcnI	CCSGG	2 cut(s)	486, 1644
BcoDI	GTCTC	4 cut(s)	87, 154, 663, 1019
BcuI	ACTAGT	1 cut(s)	1648
BfmI	CTRYAG	4 cut(s)	468, 1161, 1353, 2058
BfoI	RGCGCY	1 cut(s)	536
BfuAI	ACCTGC	3 cut(s)	461, 1064, 1183
BfuI	GTATCC	2 cut(s)	114, 1694
BglII	AGATCT	1 cut(s)	1102
BisI	GCNGC	7 cut(s)	384, 750, 1353, 1440, 1464, 1503, 1955
BlnI	CCTAGG	1 cut(s)	1696
BlsI	GCNGC	7 cut(s)	385, 751, 1354, 1441, 1465, 1504, 1956
Bme1390I	CCNGG	7 cut(s)	46, 486, 568, 796, 1305, 1335, 1644
Bme18I	GGWCC	4 cut(s)	619, 1195, 1286, 1575
BmeRI	GACNNNNNGTC	1 cut(s)	1573
BmeT110I	CYCGRG	1 cut(s)	893
BmgT120I	GGNCC	5 cut(s)	619, 1167, 1195, 1286, 1575
BmiI	GGNNCC	4 cut(s)	534, 814, 1168, 1443
BmrFI	CCNGG	7 cut(s)	46, 486, 568, 796, 1305, 1335, 1644
BmrI	ACTGGG	1 cut(s)	975
BmsI	GCATC	1 cut(s)	1981
BmuI	ACTGGG	1 cut(s)	975
BoxI	GACNNNNGTC	1 cut(s)	1031
BpiI	GAAGAC	2 cut(s)	1131, 1574
BpmI	CTGGAG	1 cut(s)	1317
BpuMI	CCSGG	2 cut(s)	486, 1644
Bsa29I	ATCGAT	1 cut(s)	1983
BsaAI	YACGTR	1 cut(s)	102
BsaHI	GRCGYC	1 cut(s)	533
BsaJI	CCNNGG	5 cut(s)	425, 567, 794, 1642, 1696
BsaWI	WCCGGW	4 cut(s)	106, 227, 1258, 1577
BsaXI	ACNNNNNCTCC	6 cut(s)	1012, 1042, 1329, 1359, 1798, 1828
Bsc4I	CCNNNNNNNGG	6 cut(s)	234, 1037, 1057, 1378, 1630, 2062
Bse1I	ACTGG	4 cut(s)	437, 970, 978, 1741
Bse21I	CCTNAGG	1 cut(s)	1998
Bse3DI	GCAATG	2 cut(s)	696, 702
BseAI	TCCGGA	1 cut(s)	106
BseBI	CCWGG	5 cut(s)	46, 568, 796, 1305, 1335
BseCI	ATCGAT	1 cut(s)	1983
BseDI	CCNNGG	5 cut(s)	425, 567, 794, 1642, 1696
BseGI	GGATG	2 cut(s)	1506, 1864
BseLI	CCNNNNNNNGG	6 cut(s)	234, 1037, 1057, 1378, 1630, 2062
BseMI	GCAATG	2 cut(s)	696, 702
BseMII	CTCAG	4 cut(s)	149, 183, 1211, 2032
BseNI	ACTGG	4 cut(s)	437, 970, 978, 1741
BseRI	GAGGAG	1 cut(s)	92
BseSI	GKGCMC	1 cut(s)	1233
BseXI	GCAGC	6 cut(s)	395, 736, 1339, 1475, 1514, 1966
BsgI	GTGCAG	1 cut(s)	1629
Bsh1285I	CGRYCG	1 cut(s)	1514
BshFI	GGCC	9 cut(s)	198, 238, 375, 1169, 1517, 1614, 1634, 1793, 2066
BshNI	GGYRCC	2 cut(s)	532, 1441
BshVI	ATCGAT	1 cut(s)	1983
BsiEI	CGRYCG	1 cut(s)	1514
BsiHKAI	GWGCWC	2 cut(s)	793, 1233
BsiHKCI	CYCGRG	1 cut(s)	893
BsiSI	CCGG	8 cut(s)	86, 107, 228, 486, 816, 1259, 1578, 1644
BslFI	GGGAC	1 cut(s)	443
BslI	CCNNNNNNNGG	6 cut(s)	234, 1037, 1057, 1378, 1630, 2062
BsmAI	GTCTC	4 cut(s)	87, 154, 663, 1019
BsmBI	CGTCTC	1 cut(s)	154
BsmFI	GGGAC	1 cut(s)	443
BsnI	GGCC	9 cut(s)	198, 238, 375, 1169, 1517, 1614, 1634, 1793, 2066
BsoBI	CYCGRG	1 cut(s)	893
Bsp1286I	GDGCHC	4 cut(s)	358, 544, 793, 1233
Bsp13I	TCCGGA	1 cut(s)	106
Bsp1407I	TGTACA	1 cut(s)	555
BspACI	CCGC	2 cut(s)	1439, 1542
BspANI	GGCC	9 cut(s)	198, 238, 375, 1169, 1517, 1614, 1634, 1793, 2066
BspCNI	CTCAG	4 cut(s)	150, 182, 1212, 2031
BspDI	ATCGAT	1 cut(s)	1983
BspEI	TCCGGA	1 cut(s)	106
BspHI	TCATGA	1 cut(s)	1521
BspLI	GGNNCC	4 cut(s)	534, 814, 1168, 1443
BspMAI	CTGCAG	2 cut(s)	472, 1357
BspMI	ACCTGC	3 cut(s)	461, 1064, 1183
BspPI	GGATC	4 cut(s)	354, 806, 984, 1210
BspT107I	GGYRCC	2 cut(s)	532, 1441
BsrDI	GCAATG	2 cut(s)	696, 702
BsrGI	TGTACA	1 cut(s)	555
BsrI	ACTGG	4 cut(s)	437, 970, 978, 1741
BssECI	CCNNGG	5 cut(s)	425, 567, 794, 1642, 1696
BssNI	GRCGYC	1 cut(s)	533
BssSI	CACGAG	1 cut(s)	537
BssT1I	CCWWGG	1 cut(s)	1696
Bst2BI	CACGAG	1 cut(s)	537
Bst2UI	CCWGG	5 cut(s)	46, 568, 796, 1305, 1335
Bst4CI	ACNGT	7 cut(s)	35, 66, 657, 773, 808, 1915, 2012
Bst6I	CTCTTC	1 cut(s)	1282
BstACI	GRCGYC	1 cut(s)	533
BstAUI	TGTACA	1 cut(s)	555
BstBAI	YACGTR	1 cut(s)	102
BstC8I	GCNNGC	5 cut(s)	183, 609, 613, 1350, 1612
BstDEI	CTNAG	5 cut(s)	158, 169, 1220, 1998, 2018
BstDSI	CCRYGG	1 cut(s)	425
BstEII	GGTNACC	1 cut(s)	473
BstF5I	GGATG	2 cut(s)	1506, 1864
BstH2I	RGCGCY	1 cut(s)	536
BstHHI	GCGC	2 cut(s)	491, 535
BstMAI	GTCTC	4 cut(s)	87, 154, 663, 1019
BstMCI	CGRYCG	1 cut(s)	1514
BstMWI	GCNNNNNNNGC	6 cut(s)	95, 539, 548, 950, 1166, 1460
BstNI	CCWGG	5 cut(s)	46, 568, 796, 1305, 1335
BstPAI	GACNNNNGTC	1 cut(s)	1031
BstPI	GGTNACC	1 cut(s)	473
BstSCI	CCNGG	7 cut(s)	44, 484, 566, 794, 1303, 1333, 1642
BstSFI	CTRYAG	4 cut(s)	468, 1161, 1353, 2058
BstSLI	GKGCMC	1 cut(s)	1233
BstV1I	GCAGC	6 cut(s)	395, 736, 1339, 1475, 1514, 1966
BstV2I	GAAGAC	2 cut(s)	1131, 1574
BstX2I	RGATCY	2 cut(s)	1102, 1215
BstXI	CCANNNNNNTGG	3 cut(s)	288, 432, 1454
BstYI	RGATCY	2 cut(s)	1102, 1215
Bsu15I	ATCGAT	1 cut(s)	1983
Bsu36I	CCTNAGG	1 cut(s)	1998
BsuI	GTATCC	2 cut(s)	114, 1694
BsuRI	GGCC	9 cut(s)	198, 238, 375, 1169, 1517, 1614, 1634, 1793, 2066
BsuTUI	ATCGAT	1 cut(s)	1983
BtgI	CCRYGG	1 cut(s)	425
BtsCI	GGATG	2 cut(s)	1506, 1864
BtsI	GCAGTG	1 cut(s)	837
BtsIMutI	CAGTG	6 cut(s)	46, 653, 837, 845, 971, 1748
BveI	ACCTGC	3 cut(s)	461, 1064, 1183
Cac8I	GCNNGC	5 cut(s)	183, 609, 613, 1350, 1612
CciI	TCATGA	1 cut(s)	1521
CfoI	GCGC	2 cut(s)	491, 535
Cfr13I	GGNCC	5 cut(s)	619, 1167, 1195, 1286, 1575
ClaI	ATCGAT	1 cut(s)	1983
Csp6I	GTAC	8 cut(s)	10, 482, 556, 774, 886, 925, 1245, 1692
CspCI	CAANNNNNGTGG	2 cut(s)	510, 545
CviQI	GTAC	8 cut(s)	10, 482, 556, 774, 886, 925, 1245, 1692
DdeI	CTNAG	5 cut(s)	158, 169, 1220, 1998, 2018
DinI	GGCGCC	1 cut(s)	534
DriI	GACNNNNNGTC	1 cut(s)	1573
EaeI	YGGCCR	5 cut(s)	196, 236, 1515, 1791, 2064
Eam1104I	CTCTTC	1 cut(s)	1282
Eam1105I	GACNNNNNGTC	1 cut(s)	1573
EarI	CTCTTC	1 cut(s)	1282
Eco130I	CCWWGG	1 cut(s)	1696
Eco147I	AGGCCT	1 cut(s)	375
Eco24I	GRGCYC	2 cut(s)	358, 544
Eco47I	GGWCC	4 cut(s)	619, 1195, 1286, 1575
Eco57I	CTGAAG	1 cut(s)	294
Eco81I	CCTNAGG	1 cut(s)	1998
Eco88I	CYCGRG	1 cut(s)	893
Eco91I	GGTNACC	1 cut(s)	473
EcoO109I	RGGNCCY	1 cut(s)	1167
EcoO65I	GGTNACC	1 cut(s)	473
EcoRII	CCWGG	5 cut(s)	44, 566, 794, 1303, 1333
EcoT14I	CCWWGG	1 cut(s)	1696
EcoT22I	ATGCAT	1 cut(s)	1379
EcoT38I	GRGCYC	2 cut(s)	358, 544
EgeI	GGCGCC	1 cut(s)	534
EheI	GGCGCC	1 cut(s)	534
ErhI	CCWWGG	1 cut(s)	1696
Esp3I	CGTCTC	1 cut(s)	154
FalI	AAGNNNNNCTT	2 cut(s)	439, 471
FaqI	GGGAC	1 cut(s)	443
FbaI	TGATCA	4 cut(s)	207, 2004, 2029, 2038
FblI	GTMKAC	1 cut(s)	1044
Fnu4HI	GCNGC	7 cut(s)	384, 750, 1353, 1440, 1464, 1503, 1955
FokI	GGATG	2 cut(s)	1493, 1871
FriOI	GRGCYC	2 cut(s)	358, 544
Fsp4HI	GCNGC	7 cut(s)	384, 750, 1353, 1440, 1464, 1503, 1955
GlaI	GCGC	2 cut(s)	490, 534
GluI	GCNGC	7 cut(s)	384, 750, 1353, 1440, 1464, 1503, 1955
GsuI	CTGGAG	1 cut(s)	1317
HaeII	RGCGCY	1 cut(s)	536
HaeIII	GGCC	9 cut(s)	198, 238, 375, 1169, 1517, 1614, 1634, 1793, 2066
HapII	CCGG	8 cut(s)	86, 107, 228, 486, 816, 1259, 1578, 1644
HhaI	GCGC	2 cut(s)	491, 535
Hin1I	GRCGYC	1 cut(s)	533
Hin6I	GCGC	2 cut(s)	489, 533
HinP1I	GCGC	2 cut(s)	489, 533
HincII	GTYRAC	2 cut(s)	462, 1045
HindII	GTYRAC	2 cut(s)	462, 1045
HpaII	CCGG	8 cut(s)	86, 107, 228, 486, 816, 1259, 1578, 1644
HphI	GGTGA	7 cut(s)	269, 414, 1248, 1475, 1930, 2014, 2024
Hpy166II	GTNNAC	7 cut(s)	462, 904, 1045, 1231, 1387, 1483, 1746
Hpy188I	TCNGA	7 cut(s)	70, 274, 503, 583, 634, 1511, 2021
Hpy8I	GTNNAC	7 cut(s)	462, 904, 1045, 1231, 1387, 1483, 1746
HpyAV	CCTTC	4 cut(s)	686, 844, 869, 1051
HpyCH4III	ACNGT	7 cut(s)	35, 66, 657, 773, 808, 1915, 2012
HpyCH4IV	ACGT	2 cut(s)	101, 1851
HpyF10VI	GCNNNNNNNGC	6 cut(s)	95, 539, 548, 950, 1166, 1460
HpyF3I	CTNAG	5 cut(s)	158, 169, 1220, 1998, 2018
HpySE526I	ACGT	2 cut(s)	101, 1851
Hsp92I	GRCGYC	1 cut(s)	533
HspAI	GCGC	2 cut(s)	489, 533
KasI	GGCGCC	1 cut(s)	532
Kpn2I	TCCGGA	1 cut(s)	106
Ksp22I	TGATCA	4 cut(s)	207, 2004, 2029, 2038
LmnI	GCTCC	5 cut(s)	146, 788, 812, 1336, 1999
Lsp1109I	GCAGC	6 cut(s)	395, 736, 1339, 1475, 1514, 1966
LweI	GCATC	1 cut(s)	1981
MaeII	ACGT	2 cut(s)	101, 1851
MaeIII	GTNAC	7 cut(s)	449, 473, 651, 969, 1357, 1402, 1843
MboII	GAAGA	5 cut(s)	1084, 1136, 1269, 1574, 1789
MflI	RGATCY	2 cut(s)	1102, 1215
MhlI	GDGCHC	4 cut(s)	358, 544, 793, 1233
MlsI	TGGCCA	4 cut(s)	198, 238, 1793, 2066
MluCI	AATT	8 cut(s)	71, 390, 627, 732, 1237, 1390, 1546, 1924
MluNI	TGGCCA	4 cut(s)	198, 238, 1793, 2066
Mly113I	GGCGCC	1 cut(s)	533
MlyI	GAGTC	9 cut(s)	89, 119, 572, 844, 1021, 1050, 1347, 1504, 1805
Mox20I	TGGCCA	4 cut(s)	198, 238, 1793, 2066
Mph1103I	ATGCAT	1 cut(s)	1379
MroI	TCCGGA	1 cut(s)	106
MscI	TGGCCA	4 cut(s)	198, 238, 1793, 2066
MseI	TTAA	3 cut(s)	393, 1212, 2070
MslI	CAYNNNNRTG	1 cut(s)	1664
Msp20I	TGGCCA	4 cut(s)	198, 238, 1793, 2066
MspI	CCGG	8 cut(s)	86, 107, 228, 486, 816, 1259, 1578, 1644
MspR9I	CCNGG	7 cut(s)	46, 486, 568, 796, 1305, 1335, 1644
MvaI	CCWGG	5 cut(s)	46, 568, 796, 1305, 1335
MwoI	GCNNNNNNNGC	6 cut(s)	95, 539, 548, 950, 1166, 1460
NarI	GGCGCC	1 cut(s)	533
NciI	CCSGG	2 cut(s)	486, 1644
NlaIV	GGNNCC	4 cut(s)	534, 814, 1168, 1443
NmuCI	GTSAC	5 cut(s)	449, 651, 969, 1402, 1843
NsiI	ATGCAT	1 cut(s)	1379
PagI	TCATGA	1 cut(s)	1521
PaqCI	CACCTGC	1 cut(s)	1064
PceI	AGGCCT	1 cut(s)	375
PcsI	WCGNNNNNNNCGW	1 cut(s)	1848
PfeI	GAWTC	3 cut(s)	216, 419, 1089
PfoI	TCCNGGA	1 cut(s)	1333
PkrI	GCNGC	7 cut(s)	385, 751, 1354, 1441, 1465, 1504, 1956
Ple19I	CGATCG	1 cut(s)	1514
PleI	GAGTC	9 cut(s)	88, 118, 572, 844, 1021, 1049, 1346, 1503, 1805
PluTI	GGCGCC	1 cut(s)	536
PpsI	GAGTC	9 cut(s)	88, 118, 572, 844, 1021, 1049, 1346, 1503, 1805
Ppu21I	YACGTR	1 cut(s)	102
PshAI	GACNNNNGTC	1 cut(s)	1031
PshBI	ATTAAT	1 cut(s)	2070
PsiI	TTATAA	1 cut(s)	1395
Psp6I	CCWGG	5 cut(s)	44, 566, 794, 1303, 1333
PspEI	GGTNACC	1 cut(s)	473
PspGI	CCWGG	5 cut(s)	44, 566, 794, 1303, 1333
PspN4I	GGNNCC	4 cut(s)	534, 814, 1168, 1443
PspPI	GGNCC	5 cut(s)	619, 1167, 1195, 1286, 1575
PsrI	GAACNNNNNNTAC	2 cut(s)	1464, 1496
PstI	CTGCAG	2 cut(s)	472, 1357
PsuI	RGATCY	2 cut(s)	1102, 1215
PvuI	CGATCG	1 cut(s)	1514
RsaI	GTAC	8 cut(s)	11, 483, 557, 775, 887, 926, 1246, 1693
RsaNI	GTAC	8 cut(s)	10, 482, 556, 774, 886, 925, 1245, 1692
RseI	CAYNNNNRTG	1 cut(s)	1664
SalI	GTCGAC	1 cut(s)	1043
SaqAI	TTAA	3 cut(s)	393, 1212, 2070
SatI	GCNGC	7 cut(s)	384, 750, 1353, 1440, 1464, 1503, 1955
Sau96I	GGNCC	5 cut(s)	619, 1167, 1195, 1286, 1575
SchI	GAGTC	9 cut(s)	89, 119, 572, 844, 1021, 1050, 1347, 1504, 1805
ScrFI	CCNGG	7 cut(s)	46, 486, 568, 796, 1305, 1335, 1644
SduI	GDGCHC	4 cut(s)	358, 544, 793, 1233
SfaNI	GCATC	1 cut(s)	1981
SfcI	CTRYAG	4 cut(s)	468, 1161, 1353, 2058
SfoI	GGCGCC	1 cut(s)	534
SinI	GGWCC	4 cut(s)	619, 1195, 1286, 1575
SmiMI	CAYNNNNRTG	1 cut(s)	1664
SpeI	ACTAGT	1 cut(s)	1648
Sse9I	AATT	8 cut(s)	71, 390, 627, 732, 1237, 1390, 1546, 1924
SseBI	AGGCCT	1 cut(s)	375
SsiI	CCGC	2 cut(s)	1439, 1542
SspDI	GGCGCC	1 cut(s)	532
StuI	AGGCCT	1 cut(s)	375
StyD4I	CCNGG	7 cut(s)	44, 484, 566, 794, 1303, 1333, 1642
StyI	CCWWGG	1 cut(s)	1696
TaaI	ACNGT	7 cut(s)	35, 66, 657, 773, 808, 1915, 2012
TaiI	ACGT	2 cut(s)	104, 1854
TaqI	TCGA	6 cut(s)	358, 417, 723, 1044, 1341, 1983
TasI	AATT	8 cut(s)	71, 390, 627, 732, 1237, 1390, 1546, 1924
TatI	WGTACW	4 cut(s)	9, 555, 773, 924
TauI	GCSGC	1 cut(s)	1442
TfiI	GAWTC	3 cut(s)	216, 419, 1089
Tru1I	TTAA	3 cut(s)	393, 1212, 2070
Tru9I	TTAA	3 cut(s)	393, 1212, 2070
TscAI	CASTG	6 cut(s)	46, 660, 844, 852, 978, 1748
TseFI	GTSAC	5 cut(s)	449, 651, 969, 1402, 1843
TseI	GCWGC	6 cut(s)	383, 749, 1352, 1463, 1502, 1954
Tsp45I	GTSAC	5 cut(s)	449, 651, 969, 1402, 1843
TspDTI	ATGAA	5 cut(s)	1084, 1428, 1652, 1676, 1733
TspGWI	ACGGA	1 cut(s)	178
TspRI	CASTG	6 cut(s)	46, 660, 844, 852, 978, 1748
VneI	GTGCAC	1 cut(s)	1229
VpaK11BI	GGWCC	4 cut(s)	619, 1195, 1286, 1575
VspI	ATTAAT	1 cut(s)	2070
XapI	RAATTY	1 cut(s)	1237
XbaI	TCTAGA	2 cut(s)	853, 1885
XmaJI	CCTAGG	1 cut(s)	1696
XmiI	GTMKAC	1 cut(s)	1044
Zsp2I	ATGCAT	1 cut(s)	1379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.