MD08G1098000.v1.1

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002861
Physical Location & Seq
Reverse (-)
8228576 .. 8229283
708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1098000.v1.1.491

Sequence Viewer

Length: 708 bp
ATGCTGCTGCAGATCAACGATGCAGTATCGAATGTTGAGTCCGAATCTTTCTCAACGCACGAGGCGTGGCAGAAGTCCACCTTAAAATCAGTGTCGTCTTCGTCTCCTGCAGAAAATGATGACAGTGAAATGTTGCTGTACTCATACAGCCATGTCGTGCAGGGATTCAGTGCCAGGCTTACTCCTCAGCTGTCTAGATTGGAGATATCCCCAGCTCATGTTGCCATGTATCCCAAGTCTTTTGGAAAGATGTTCACAATTCACAGTCCCAAGTTTCTTGGACTTAGACAGAACCTCGGCTCACGGCCTAATGCATCATTTGGCCAAGATGTGATATTAGGAATTATTGATACAGGAATTTGGCCAGGGAGTGAGAGCTTTGGCGATAAGGGAATGTCAGAAGAGCCAATAGGCGATAAAGGAATGTCAGAAGTGCCAATAGGATGGAAAGGCGAGTGTGAGAATGGAACTGCATTCACCCCGTCTCTCTGCAATAAGAAGCTCATTCGTGCTCTATCTTTCAGCAAAGGGCAGCTGCAGACCAAAGATCATGACTATGATTTGCCGAGAGACTTCTTTGGTCACAGAACACACACACCATCGACAGCTGCAGGCAACCATGTATCATTTCTGATTCGCCGAGAGACTTCTTTGGTCACGGAACACACACATCATCGTCTGCTGCAGGCAACCATGTGTAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

236

Amino Acids

26.02

Weight (kDa)

6.4

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 14 - 63 1.7e-08 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 322, 362
AcsI RAATTY 1 cut(s) 357
AfaI GTAC 1 cut(s) 140
AjnI CCWGG 2 cut(s) 173, 364
AluBI AGCT 6 cut(s) 190, 215, 378, 502, 535, 608
AluI AGCT 6 cut(s) 190, 215, 378, 502, 535, 608
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 4 cut(s) 108, 489, 564, 638
AoxI GGCC 3 cut(s) 305, 322, 362
ApeKI GCWGC 6 cut(s) 4, 7, 532, 535, 608, 682
ApoI RAATTY 1 cut(s) 357
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AsuHPI GGTGA 1 cut(s) 469
BalI TGGCCA 2 cut(s) 324, 364
BauI CACGAG 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 514
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 4 cut(s) 522, 544, 595, 669
BccI CCATC 2 cut(s) 438, 607
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 2 cut(s) 175, 366
BciVI GTATCC 1 cut(s) 240
BcoDI GTCTC 4 cut(s) 108, 489, 564, 638
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 5 cut(s) 8, 108, 536, 609, 683
BfuI GTATCC 1 cut(s) 240
BisI GCNGC 6 cut(s) 5, 8, 533, 536, 609, 683
BlsI GCNGC 6 cut(s) 6, 9, 534, 537, 610, 684
Bme1390I CCNGG 2 cut(s) 175, 366
BmrFI CCNGG 2 cut(s) 175, 366
BmsI GCATC 2 cut(s) 10, 323
BpiI GAAGAC 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 295, 365
BseBI CCWGG 2 cut(s) 175, 366
BseDI CCNNGG 2 cut(s) 295, 365
BseGI GGATG 1 cut(s) 449
BseMII CTCAG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 4 cut(s) 522, 544, 595, 669
BseYI CCCAGC 1 cut(s) 211
BsgI GTGCAG 1 cut(s) 179
BshFI GGCC 3 cut(s) 307, 324, 364
BsiHKAI GWGCWC 1 cut(s) 514
BslFI GGGAC 1 cut(s) 252
BsmAI GTCTC 4 cut(s) 108, 489, 564, 638
BsmBI CGTCTC 2 cut(s) 108, 489
BsmFI GGGAC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 3 cut(s) 307, 324, 364
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 2 cut(s) 12, 547
BspANI GGCC 3 cut(s) 307, 324, 364
BspCNI CTCAG 1 cut(s) 199
BspHI TCATGA 1 cut(s) 550
BspMAI CTGCAG 5 cut(s) 12, 112, 540, 613, 687
BspQI GCTCTTC 1 cut(s) 396
BssECI CCNNGG 2 cut(s) 295, 365
BssMI GATC 2 cut(s) 12, 547
BssSI CACGAG 1 cut(s) 59
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 175, 366
Bst4CI ACNGT 2 cut(s) 125, 266
Bst6I CTCTTC 1 cut(s) 396
BstC8I GCNNGC 2 cut(s) 613, 687
BstDEI CTNAG 2 cut(s) 186, 284
BstF5I GGATG 1 cut(s) 449
BstKTI GATC 2 cut(s) 15, 550
BstMAI GTCTC 4 cut(s) 108, 489, 564, 638
BstMBI GATC 2 cut(s) 12, 547
BstMWI GCNNNNNNNGC 1 cut(s) 221
BstNI CCWGG 2 cut(s) 175, 366
BstSCI CCNGG 2 cut(s) 173, 364
BstSFI CTRYAG 5 cut(s) 8, 108, 536, 609, 683
BstV1I GCAGC 4 cut(s) 522, 544, 595, 669
BstV2I GAAGAC 1 cut(s) 90
BstXI CCANNNNNNTGG 1 cut(s) 444
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 3 cut(s) 307, 324, 364
BtsCI GGATG 1 cut(s) 449
BtsIMutI CAGTG 3 cut(s) 96, 130, 175
Cac8I GCNNGC 2 cut(s) 613, 687
CciI TCATGA 1 cut(s) 550
Csp6I GTAC 1 cut(s) 139
CviAII CATG 6 cut(s) 152, 218, 226, 551, 620, 694
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 186, 284
DpnI GATC 2 cut(s) 14, 549
DpnII GATC 2 cut(s) 12, 547
EaeI YGGCCR 2 cut(s) 322, 362
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco32I GATATC 1 cut(s) 207
EcoRII CCWGG 2 cut(s) 173, 364
EcoRV GATATC 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 316
Esp3I CGTCTC 2 cut(s) 108, 489
FaeI CATG 6 cut(s) 155, 221, 229, 554, 623, 697
FaiI YATR 8 cut(s) 145, 153, 219, 227, 552, 558, 621, 695
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 1 cut(s) 252
FatI CATG 6 cut(s) 151, 217, 225, 550, 619, 693
Fnu4HI GCNGC 6 cut(s) 5, 8, 533, 536, 609, 683
FokI GGATG 1 cut(s) 456
Fsp4HI GCNGC 6 cut(s) 5, 8, 533, 536, 609, 683
FspBI CTAG 1 cut(s) 195
GluI GCNGC 6 cut(s) 5, 8, 533, 536, 609, 683
GsaI CCCAGC 1 cut(s) 215
HaeIII GGCC 3 cut(s) 307, 324, 364
Hin1II CATG 6 cut(s) 155, 221, 229, 554, 623, 697
HinfI GANTC 4 cut(s) 38, 44, 165, 634
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 2 cut(s) 78, 255
Hpy188I TCNGA 5 cut(s) 43, 400, 430, 633, 707
Hpy188III TCNNGA 2 cut(s) 195, 551
Hpy8I GTNNAC 2 cut(s) 78, 255
HpyCH4III ACNGT 2 cut(s) 125, 266
HpyF10VI GCNNNNNNNGC 1 cut(s) 221
HpyF3I CTNAG 2 cut(s) 186, 284
Hsp92II CATG 6 cut(s) 155, 221, 229, 554, 623, 697
Kzo9I GATC 2 cut(s) 12, 547
LguI GCTCTTC 1 cut(s) 396
Lsp1109I GCAGC 4 cut(s) 522, 544, 595, 669
LweI GCATC 2 cut(s) 10, 323
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 581, 655
MalI GATC 2 cut(s) 14, 549
MboI GATC 2 cut(s) 12, 547
MboII GAAGA 2 cut(s) 90, 413
MhlI GDGCHC 1 cut(s) 514
MlsI TGGCCA 2 cut(s) 324, 364
MluCI AATT 4 cut(s) 258, 342, 357, 700
MluNI TGGCCA 2 cut(s) 324, 364
MlyI GAGTC 1 cut(s) 47
MnlI CCTC 3 cut(s) 55, 195, 305
Mox20I TGGCCA 2 cut(s) 324, 364
Mph1103I ATGCAT 1 cut(s) 316
MscI TGGCCA 2 cut(s) 324, 364
MseI TTAA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 2 cut(s) 324, 364
MspA1I CMGCKG 3 cut(s) 190, 535, 608
MspR9I CCNGG 2 cut(s) 175, 366
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 2 cut(s) 175, 366
MwoI GCNNNNNNNGC 1 cut(s) 221
NdeII GATC 2 cut(s) 12, 547
NlaIII CATG 6 cut(s) 155, 221, 229, 554, 623, 697
NmeAIII GCCGAG 3 cut(s) 276, 591, 665
NmuCI GTSAC 2 cut(s) 581, 655
NsiI ATGCAT 1 cut(s) 316
PagI TCATGA 1 cut(s) 550
PciSI GCTCTTC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PctI GAATGC 1 cut(s) 473
PfeI GAWTC 3 cut(s) 44, 165, 634
PkrI GCNGC 6 cut(s) 6, 9, 534, 537, 610, 684
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 173, 364
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 2 cut(s) 173, 364
PstI CTGCAG 5 cut(s) 12, 112, 540, 613, 687
PvuII CAGCTG 3 cut(s) 190, 535, 608
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 555
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 6 cut(s) 5, 8, 533, 536, 609, 683
Sau3AI GATC 2 cut(s) 12, 547
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 175, 366
SduI GDGCHC 1 cut(s) 514
SetI ASST 8 cut(s) 83, 192, 217, 297, 380, 504, 537, 610
SfaNI GCATC 2 cut(s) 10, 323
SfcI CTRYAG 5 cut(s) 8, 108, 536, 609, 683
SmiMI CAYNNNNRTG 1 cut(s) 555
Sse9I AATT 4 cut(s) 258, 342, 357, 700
SspMI CTAG 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 173, 364
TaaI ACNGT 2 cut(s) 125, 266
TaqI TCGA 2 cut(s) 29, 602
TasI AATT 4 cut(s) 258, 342, 357, 700
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 3 cut(s) 44, 165, 634
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 3 cut(s) 96, 130, 175
TseFI GTSAC 2 cut(s) 581, 655
TseI GCWGC 6 cut(s) 4, 7, 532, 535, 608, 682
Tsp45I GTSAC 2 cut(s) 581, 655
TspGWI ACGGA 1 cut(s) 674
TspRI CASTG 3 cut(s) 96, 130, 175
XapI RAATTY 1 cut(s) 357
XbaI TCTAGA 1 cut(s) 194
XspI CTAG 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.