MD15G1080200.v1.1

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002861
Physical Location & Seq
Reverse (-)
5451448 .. 5452074
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1080200.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGATTTGTCCAGCATGACCACACTTCTGTTGTTAACGCTGTTGCAGATCAACAATGCAGTATCGAATGTTGAGGAAATCCAGACGTACATCATTCACATGGATCATTCCCACAAACCCGAATCGTTCTTAACGCACGAAGCTTGGCACAAGTCCACCGTAAAGTCATTGTCGTCTTCGTCTCCTGCAGAAAACGGTGATGGCAGTGAAATGTTGCTGTACTCATACAGCCATGTCATGCATGGGTTCAGTGCAAGGCTCACTCCTTCTCAGCTGTCTAGATTGGAGAAATCCCCGGCTCATGTTGCCACGTATCCCGAGTTTTTTGGCAAGATGTTCACAACTCACGGCCCTAAGTTTCTTGGACTTAGGCAGAATCTCGGTTTATGGCCTAATGCCTCATTTGGCCAAGATGTGATCATAGGTATTCTTGATTCAGGAATTTGGCCAGAAAGTGAAAGTTTCGGCGATAAGGGAATGTCAGAGGTGCCATTAAGATGGAAAGGCGTGTGCGAGAATGGAACTGCATTTACCCCGTCTCTCTGCAACAAGAAGCTCATTGGTGCTCGATCTTTCAGCAAAGGGCACAAGGCTGCAGGTATACCGATTAGCAAAGAATATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

209

Amino Acids

22.94

Weight (kDa)

6.55

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 29 - 107 9.1e-17 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 601
AclWI GGATC 1 cut(s) 111
AcoI YGGCCR 2 cut(s) 406, 446
AcsI RAATTY 1 cut(s) 441
AfaI GTAC 2 cut(s) 89, 221
AluBI AGCT 3 cut(s) 143, 274, 556
AluI AGCT 3 cut(s) 143, 274, 556
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 186, 543
AlwI GGATC 1 cut(s) 111
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 4 cut(s) 349, 389, 406, 446
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 1 cut(s) 441
AspS9I GGNCC 1 cut(s) 350
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 317
BaeGI GKGCMC 1 cut(s) 588
BalI TGGCCA 2 cut(s) 408, 448
BanI GGYRCC 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 568
BbvI GCAGC 1 cut(s) 580
BccI CCATC 2 cut(s) 194, 492
BceAI ACGGC 1 cut(s) 364
BciVI GTATCC 1 cut(s) 324
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 186, 543
BfaI CTAG 2 cut(s) 279, 625
BfmI CTRYAG 2 cut(s) 186, 594
BfuAI ACCTGC 1 cut(s) 587
BfuI GTATCC 1 cut(s) 324
BisI GCNGC 1 cut(s) 594
BlsI GCNGC 1 cut(s) 595
Bme1390I CCNGG 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 1 cut(s) 296
BpiI GAAGAC 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 296
BsaAI YACGTR 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 284
BseSI GKGCMC 1 cut(s) 588
BseXI GCAGC 1 cut(s) 580
BshFI GGCC 4 cut(s) 351, 391, 408, 448
BshNI GGYRCC 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 568
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 1 cut(s) 296
BsmAI GTCTC 2 cut(s) 186, 543
BsmBI CGTCTC 2 cut(s) 186, 543
BsnI GGCC 4 cut(s) 351, 391, 408, 448
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 568, 588
Bsp143I GATC 4 cut(s) 48, 103, 417, 569
BspANI GGCC 4 cut(s) 351, 391, 408, 448
BspCNI CTCAG 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 489
BspMAI CTGCAG 2 cut(s) 190, 598
BspMI ACCTGC 1 cut(s) 587
BspPI GGATC 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 487
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 4 cut(s) 48, 103, 417, 569
BssNAI GTATAC 1 cut(s) 602
Bst1107I GTATAC 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 160, 197
BstBAI YACGTR 1 cut(s) 312
BstDEI CTNAG 3 cut(s) 270, 354, 368
BstKTI GATC 4 cut(s) 51, 106, 420, 572
BstMAI GTCTC 2 cut(s) 186, 543
BstMBI GATC 4 cut(s) 48, 103, 417, 569
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 2 cut(s) 186, 594
BstSLI GKGCMC 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 498
BstZ17I GTATAC 1 cut(s) 602
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 4 cut(s) 351, 391, 408, 448
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 211, 256
BveI ACCTGC 1 cut(s) 587
Cfr13I GGNCC 1 cut(s) 350
Csp6I GTAC 2 cut(s) 88, 220
CviAII CATG 6 cut(s) 16, 100, 233, 238, 242, 302
CviQI GTAC 2 cut(s) 88, 220
DdeI CTNAG 3 cut(s) 270, 354, 368
DpnI GATC 4 cut(s) 50, 105, 419, 571
DpnII GATC 4 cut(s) 48, 103, 417, 569
EaeI YGGCCR 2 cut(s) 406, 446
Eco88I CYCGRG 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 243
Esp3I CGTCTC 2 cut(s) 186, 543
FaeI CATG 6 cut(s) 19, 103, 236, 241, 245, 305
FatI CATG 6 cut(s) 15, 99, 232, 237, 241, 301
FbaI TGATCA 1 cut(s) 417
FblI GTMKAC 1 cut(s) 601
Fnu4HI GCNGC 1 cut(s) 594
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 2 cut(s) 279, 625
GluI GCNGC 1 cut(s) 594
HaeIII GGCC 4 cut(s) 351, 391, 408, 448
HapII CCGG 1 cut(s) 296
Hin1II CATG 6 cut(s) 19, 103, 236, 241, 245, 305
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 3 cut(s) 122, 376, 434
HpaI GTTAAC 1 cut(s) 36
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 4 cut(s) 36, 156, 339, 602
Hpy188I TCNGA 1 cut(s) 484
Hpy188III TCNNGA 5 cut(s) 82, 279, 317, 431, 438
Hpy8I GTNNAC 4 cut(s) 36, 156, 339, 602
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 2 cut(s) 160, 197
HpyCH4IV ACGT 2 cut(s) 86, 311
HpyCH4V TGCA 8 cut(s) 46, 59, 188, 241, 254, 527, 546, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 270, 354, 368
HpySE526I ACGT 2 cut(s) 86, 311
Hsp92II CATG 6 cut(s) 19, 103, 236, 241, 245, 305
Ksp22I TGATCA 1 cut(s) 417
KspAI GTTAAC 1 cut(s) 36
Kzo9I GATC 4 cut(s) 48, 103, 417, 569
LpnPI CCDG 7 cut(s) 25, 95, 198, 309, 423, 462, 582
Lsp1109I GCAGC 1 cut(s) 580
MaeI CTAG 2 cut(s) 279, 625
MaeII ACGT 2 cut(s) 86, 311
MalI GATC 4 cut(s) 50, 105, 419, 571
MboI GATC 4 cut(s) 48, 103, 417, 569
MboII GAAGA 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 568, 588
MlsI TGGCCA 2 cut(s) 408, 448
MluCI AATT 1 cut(s) 441
MluNI TGGCCA 2 cut(s) 408, 448
MnlI CCTC 3 cut(s) 67, 409, 478
Mox20I TGGCCA 2 cut(s) 408, 448
Mph1103I ATGCAT 1 cut(s) 243
MscI TGGCCA 2 cut(s) 408, 448
MseI TTAA 3 cut(s) 35, 131, 494
MslI CAYNNNNRTG 2 cut(s) 98, 496
Msp20I TGGCCA 2 cut(s) 408, 448
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 296
NdeII GATC 4 cut(s) 48, 103, 417, 569
NlaIII CATG 6 cut(s) 19, 103, 236, 241, 245, 305
NlaIV GGNNCC 1 cut(s) 489
NsiI ATGCAT 1 cut(s) 243
PfeI GAWTC 3 cut(s) 122, 376, 434
PkrI GCNGC 1 cut(s) 595
Ppu21I YACGTR 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 1 cut(s) 350
PsrI GAACNNNNNNTAC 2 cut(s) 514, 546
PstI CTGCAG 2 cut(s) 190, 598
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 2 cut(s) 89, 221
RsaNI GTAC 2 cut(s) 88, 220
RseI CAYNNNNRTG 2 cut(s) 98, 496
SaqAI TTAA 3 cut(s) 35, 131, 494
SatI GCNGC 1 cut(s) 594
Sau3AI GATC 4 cut(s) 48, 103, 417, 569
Sau96I GGNCC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 2 cut(s) 568, 588
SetI ASST 8 cut(s) 89, 145, 276, 314, 427, 489, 558, 601
SfcI CTRYAG 2 cut(s) 186, 594
SmiMI CAYNNNNRTG 2 cut(s) 98, 496
Sse9I AATT 1 cut(s) 441
SspMI CTAG 2 cut(s) 279, 625
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 160, 197
TaiI ACGT 2 cut(s) 89, 314
TaqI TCGA 2 cut(s) 65, 568
TasI AATT 1 cut(s) 441
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 3 cut(s) 122, 376, 434
Tru1I TTAA 3 cut(s) 35, 131, 494
Tru9I TTAA 3 cut(s) 35, 131, 494
TscAI CASTG 2 cut(s) 211, 256
TseI GCWGC 1 cut(s) 593
TspRI CASTG 2 cut(s) 211, 256
XapI RAATTY 1 cut(s) 441
XbaI TCTAGA 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 601
XspI CTAG 2 cut(s) 279, 625
Zsp2I ATGCAT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.