MD05G1275800.v1.1

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002861
Physical Location & Seq
Reverse (-)
41022714 .. 41023244
531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1275800.v1.1.491

Sequence Viewer

Length: 531 bp
ATGATCCGTGGTCTGGGCTTGTCGGTGGTGGTGGTGGTGCTGCTGTTGCTGCAGAGCAACAGCAATGCATCTGCACTATCCGAGGAATACAAGACCTATATTATCCAGATGGACTACGAATCCTCATCAGCCACGCTTGCATCTTTCCCGTCAGTGGAGGCGTGGCACAAGTCCGTCCTCAAGTCGCTGCCTTCGTCTCCTCCGGACTACGACGACAACAGTGACATGCTATTGTACTCCTACAGCCACGCCTTGCACGGCTTCAGCGCCAAACTCACTGACTCGCAGTTGTCGGAACTGGAGGACTCGCCTGCTCACGTCGCCACGTATCCGGACTCATTTGGCAGGCTGTTCACCACCCATAGCCCCAAGTTTCTCGGCCTTAATGACAAAGTTGGCGTCTGGCCTGCTTCTTCCTATGGAAAAGATGTGATAATAGGAGTGATCGATACCGGAATTTGGCCCGAGAGTGAGAGCTTCAGCGACAAGGGAATGCCACGAGTCCCAAAAAGATGGAAAGGCGCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

177

Amino Acids

19.28

Weight (kDa)

5.25

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 114 1.7e-15 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 202, 331
AcsI RAATTY 1 cut(s) 456
AcuI CTGAAG 2 cut(s) 247, 463
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 3 cut(s) 13, 154, 459
AjiI CACGTC 1 cut(s) 319
AluBI AGCT 1 cut(s) 477
AluI AGCT 1 cut(s) 477
Alw26I GTCTC 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 464
Aor13HI TCCGGA 2 cut(s) 202, 331
AoxI GGCC 3 cut(s) 379, 404, 461
ApeKI GCWGC 3 cut(s) 40, 49, 187
ApoI RAATTY 1 cut(s) 456
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
AspLEI GCGC 2 cut(s) 269, 524
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 464
BauI CACGAG 1 cut(s) 498
BbvI GCAGC 3 cut(s) 27, 36, 174
BccI CCATC 2 cut(s) 103, 507
BceAI ACGGC 1 cut(s) 274
BciVI GTATCC 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 529
BfmI CTRYAG 2 cut(s) 50, 241
BfoI RGCGCY 1 cut(s) 270
BfuI GTATCC 1 cut(s) 339
BisI GCNGC 3 cut(s) 41, 50, 188
BlsI GCNGC 3 cut(s) 42, 51, 189
BmeT110I CYCGRG 1 cut(s) 464
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 462
BmsI GCATC 2 cut(s) 77, 149
BpmI CTGGAG 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 164
Bsa29I ATCGAT 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 7, 81
BsaWI WCCGGW 3 cut(s) 202, 331, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 154, 459
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 70
BseAI TCCGGA 2 cut(s) 202, 331
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 7, 81
BseLI CCNNNNNNNGG 3 cut(s) 13, 154, 459
BseMI GCAATG 1 cut(s) 70
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 189
BseXI GCAGC 3 cut(s) 27, 36, 174
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 3 cut(s) 381, 406, 463
BshVI ATCGAT 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 3 cut(s) 203, 332, 453
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 3 cut(s) 13, 154, 459
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 488
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 3 cut(s) 381, 406, 463
BsoBI CYCGRG 1 cut(s) 464
Bsp13I TCCGGA 2 cut(s) 202, 331
Bsp143I GATC 2 cut(s) 3, 444
BspANI GGCC 3 cut(s) 381, 406, 463
BspDI ATCGAT 1 cut(s) 447
BspEI TCCGGA 2 cut(s) 202, 331
BspMAI CTGCAG 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 70
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 2 cut(s) 7, 81
BssMI GATC 2 cut(s) 3, 444
BssNI GRCGYC 1 cut(s) 399
BssSI CACGAG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 221
BstACI GRCGYC 1 cut(s) 399
BstBAI YACGTR 1 cut(s) 327
BstC8I GCNNGC 5 cut(s) 138, 312, 347, 408, 526
BstDSI CCRYGG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 269, 524
BstKTI GATC 2 cut(s) 6, 447
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 3, 444
BstMWI GCNNNNNNNGC 4 cut(s) 46, 49, 137, 320
BstNSI RCATGY 2 cut(s) 229, 528
BstSFI CTRYAG 2 cut(s) 50, 241
BstV1I GCAGC 3 cut(s) 27, 36, 174
BstXI CCANNNNNNTGG 1 cut(s) 513
Bsu15I ATCGAT 1 cut(s) 447
BsuI GTATCC 1 cut(s) 339
BsuRI GGCC 3 cut(s) 381, 406, 463
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 7
BtrI CACGTC 1 cut(s) 319
BtsIMutI CAGTG 3 cut(s) 159, 226, 276
Cac8I GCNNGC 5 cut(s) 138, 312, 347, 408, 526
CfoI GCGC 2 cut(s) 269, 524
Cfr13I GGNCC 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 447
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 235
CviAII CATG 2 cut(s) 226, 525
CviQI GTAC 1 cut(s) 235
DpnI GATC 2 cut(s) 5, 446
DpnII GATC 2 cut(s) 3, 444
Eco57I CTGAAG 2 cut(s) 247, 463
Eco88I CYCGRG 1 cut(s) 464
EcoT22I ATGCAT 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 2 cut(s) 229, 528
FaiI YATR 5 cut(s) 99, 227, 363, 420, 526
FaqI GGGAC 1 cut(s) 488
FatI CATG 2 cut(s) 225, 524
Fnu4HI GCNGC 3 cut(s) 41, 50, 188
Fsp4HI GCNGC 3 cut(s) 41, 50, 188
FspBI CTAG 1 cut(s) 529
GlaI GCGC 2 cut(s) 268, 523
GluI GCNGC 3 cut(s) 41, 50, 188
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 381, 406, 463
HapII CCGG 3 cut(s) 203, 332, 453
HgaI GACGC 1 cut(s) 388
HhaI GCGC 2 cut(s) 269, 524
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 2 cut(s) 229, 528
Hin6I GCGC 2 cut(s) 267, 522
HinP1I GCGC 2 cut(s) 267, 522
HinfI GANTC 5 cut(s) 119, 281, 305, 335, 501
HpaII CCGG 3 cut(s) 203, 332, 453
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 354
Hpy188I TCNGA 2 cut(s) 82, 295
Hpy188III TCNNGA 3 cut(s) 106, 203, 332
Hpy8I GTNNAC 1 cut(s) 354
Hpy99I CGWCG 2 cut(s) 215, 323
HpyAV CCTTC 1 cut(s) 201
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 2 cut(s) 318, 326
HpyCH4V TGCA 5 cut(s) 52, 68, 74, 140, 256
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 49, 137, 320
HpySE526I ACGT 2 cut(s) 318, 326
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 2 cut(s) 229, 528
HspAI GCGC 2 cut(s) 267, 522
Kpn2I TCCGGA 2 cut(s) 202, 331
Kzo9I GATC 2 cut(s) 3, 444
LpnPI CCDG 9 cut(s) 119, 216, 284, 324, 331, 345, 388, 420, 466
Lsp1109I GCAGC 3 cut(s) 27, 36, 174
LweI GCATC 2 cut(s) 77, 149
MaeI CTAG 1 cut(s) 529
MaeII ACGT 2 cut(s) 318, 326
MaeIII GTNAC 1 cut(s) 221
MalI GATC 2 cut(s) 5, 446
MboI GATC 2 cut(s) 3, 444
MboII GAAGA 1 cut(s) 405
MluCI AATT 1 cut(s) 456
MlyI GAGTC 4 cut(s) 275, 299, 329, 510
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 6 cut(s) 76, 133, 151, 188, 210, 295
Mph1103I ATGCAT 1 cut(s) 70
MroI TCCGGA 2 cut(s) 202, 331
MseI TTAA 1 cut(s) 384
MspI CCGG 3 cut(s) 203, 332, 453
Mva1269I GAATGC 1 cut(s) 498
MwoI GCNNNNNNNGC 4 cut(s) 46, 49, 137, 320
NdeII GATC 2 cut(s) 3, 444
NlaIII CATG 2 cut(s) 229, 528
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 221
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 2 cut(s) 229, 528
PaeI GCATGC 1 cut(s) 528
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 3 cut(s) 42, 51, 189
PleI GAGTC 4 cut(s) 275, 299, 329, 509
PpsI GAGTC 4 cut(s) 275, 299, 329, 509
Ppu21I YACGTR 1 cut(s) 327
PspPI GGNCC 1 cut(s) 462
PstI CTGCAG 1 cut(s) 54
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 3 cut(s) 41, 50, 188
Sau3AI GATC 2 cut(s) 3, 444
Sau96I GGNCC 1 cut(s) 462
SchI GAGTC 4 cut(s) 275, 299, 329, 510
SetI ASST 4 cut(s) 98, 321, 329, 479
SfaNI GCATC 2 cut(s) 77, 149
SfcI CTRYAG 2 cut(s) 50, 241
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SphI GCATGC 1 cut(s) 528
Sse9I AATT 1 cut(s) 456
SspMI CTAG 1 cut(s) 529
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 2 cut(s) 321, 329
TaqI TCGA 1 cut(s) 447
TasI AATT 1 cut(s) 456
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 3 cut(s) 159, 226, 283
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 3 cut(s) 40, 49, 187
Tsp45I GTSAC 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 3 cut(s) 159, 226, 283
XapI RAATTY 1 cut(s) 456
XceI RCATGY 2 cut(s) 229, 528
XspI CTAG 1 cut(s) 529
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.