Rorug01G0066300

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002861
Physical Location & Seq
Forward (+)
10746872 .. 10747273
402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0066300.1

Sequence Viewer

Length: 402 bp
ATGGATCTGGTAGGGGGTTACTATGATGCTGGAGACAACGTGAAGTTCAACTTTCCCATGGCATTCACAACAACAATGTTGTCTTGGAGTGTTCTAGAGTTTGGTGAATCAATGGGCTCAGAACTTCCATATGCTTCGGAAGCTATAAGATGGGGCACTGATTACTTGCTTAAATCCACAGATGCTTCTGGTTCTGTTGCCGGCTTTGTTGGCGACCCCGTAGGCGACCACACTTGTTGGGAAAGACCCGAAGATATGGACACTCCTCGAACATCCTACATAGTAACCAGAGAAAAGCCAGGCAAAGAAGTATCTGCCGAAACGCCGAAACTGCAGCTGCCCTTGCAGCTTCTTCCATGGTTTTACAGGTTCGGACGACTGGCACTCTGGTTTGCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

133

Amino Acids

14.93

Weight (kDa)

4.57

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 108 5.7e-40 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 12
AgsI TTSAA 1 cut(s) 49
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 3 cut(s) 143, 337, 349
AluI AGCT 3 cut(s) 143, 337, 349
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 3 cut(s) 334, 337, 346
AsuHPI GGTGA 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 119
BbvI GCAGC 3 cut(s) 324, 346, 358
BccI CCATC 1 cut(s) 144
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 332
BisI GCNGC 3 cut(s) 335, 338, 347
BlsI GCNGC 3 cut(s) 336, 339, 348
Bme1390I CCNGG 1 cut(s) 300
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 2 cut(s) 16, 172
BpmI CTGGAG 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 57, 356
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 57, 356
BseGI GGATG 1 cut(s) 272
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 255
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 3 cut(s) 324, 346, 358
BsiSI CCGG 1 cut(s) 201
BsmAI GTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 119, 158
Bsp143I GATC 1 cut(s) 4
Bsp19I CCATGG 2 cut(s) 57, 356
BspCNI CTCAG 1 cut(s) 131
BspMAI CTGCAG 1 cut(s) 336
BspPI GGATC 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 384
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 57, 356
BssMI GATC 1 cut(s) 4
BssT1I CCWWGG 2 cut(s) 57, 356
Bst2UI CCWGG 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 1 cut(s) 118
BstDSI CCRYGG 2 cut(s) 57, 356
BstF5I GGATG 1 cut(s) 272
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 5 cut(s) 140, 210, 331, 343, 346
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 332
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 324, 346, 358
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtgI CCRYGG 2 cut(s) 57, 356
BtsCI GGATG 1 cut(s) 272
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 200
CspCI CAANNNNNGTGG 2 cut(s) 218, 253
CviAII CATG 2 cut(s) 58, 357
CviJI RGCY 6 cut(s) 117, 143, 204, 298, 337, 349
CviKI_1 RGCY 6 cut(s) 117, 143, 204, 298, 337, 349
DdeI CTNAG 1 cut(s) 118
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eco130I CCWWGG 2 cut(s) 57, 356
Eco24I GRGCYC 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 57, 356
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 2 cut(s) 57, 356
FaeI CATG 2 cut(s) 61, 360
FaiI YATR 8 cut(s) 24, 59, 130, 132, 146, 257, 281, 358
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FatI CATG 2 cut(s) 57, 356
FauNDI CATATG 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 335, 338, 347
FokI GGATG 1 cut(s) 259
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 3 cut(s) 335, 338, 347
FspBI CTAG 1 cut(s) 95
GluI GCNGC 3 cut(s) 335, 338, 347
GsuI CTGGAG 1 cut(s) 51
HapII CCGG 1 cut(s) 201
Hin1II CATG 2 cut(s) 61, 360
HinfI GANTC 1 cut(s) 107
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 116
Hpy188I TCNGA 3 cut(s) 121, 139, 374
Hpy188III TCNNGA 1 cut(s) 95
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 2 cut(s) 334, 346
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 210, 331, 343, 346
HpyF3I CTNAG 1 cut(s) 118
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 2 cut(s) 61, 360
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 15, 174, 214, 285, 301, 312, 352, 365, 373
Lsp1109I GCAGC 3 cut(s) 324, 346, 358
LweI GCATC 2 cut(s) 16, 172
MaeI CTAG 1 cut(s) 95
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 2 cut(s) 17, 283
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 2 cut(s) 263, 344
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 119, 158
MnlI CCTC 1 cut(s) 276
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 1 cut(s) 171
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 5 cut(s) 140, 210, 331, 343, 346
NaeI GCCGGC 1 cut(s) 202
NcoI CCATGG 2 cut(s) 57, 356
NdeI CATATG 1 cut(s) 130
NdeII GATC 1 cut(s) 4
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 2 cut(s) 61, 360
PctI GAATGC 1 cut(s) 62
PdiI GCCGGC 1 cut(s) 202
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 336, 339, 348
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PstI CTGCAG 1 cut(s) 336
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 337
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 3 cut(s) 335, 338, 347
Sau3AI GATC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 2 cut(s) 119, 158
SetI ASST 5 cut(s) 42, 145, 339, 351, 371
SfaNI GCATC 2 cut(s) 16, 172
SfcI CTRYAG 1 cut(s) 332
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 2 cut(s) 57, 356
TaiI ACGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 268
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 1 cut(s) 163
TseI GCWGC 3 cut(s) 334, 337, 346
TspRI CASTG 1 cut(s) 163
XbaI TCTAGA 1 cut(s) 94
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.