Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002861

Physical Location & Seq

Genomic Coordinates Reverse (-)

41020938 .. 41022494

Gene Structure 1557 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1275700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGCGCCGCGTGCACACCTTGCCATGTACAAAATCGGATGGTCTGGACAAGAACTTATAGCTAGTGATGTGCTAGCTGGCATGGACAGGGCCATTTCCGATGGCGTTGATATCATGTCCCTTTCTTTTGGCTTATATATACCCGAATATTTCAACGATGTGATTGCGATCGCTTCTTTCTCAGCAGTCCAAAGAGGAATTGTTGTCGTGTGTGCTGTGGGAAATGACGGTCCGCTTGAAAGCTCAACATCCAACGGAGCCCCGTGGATCACTACAGTAGGTGCCGGAACAATGGATCGTACTTTTACTGCAAAATTTACGCTCGATGGTGGCTTATTTACCGCTGAAGGAGTATCATACTTCCCGCTAAGCGTTTACATTAGTGATGCACCTTTGTTTTATGGGAAACACAATACAAGCAAACAAAGATGTGACCCTGGGGCATTGATTCGTGAAGAAGTGGTCAGCAAGGTGGTTCTTTGTGATTGGAGCAACCTGACTTCCTTCGTTGGTCAAATGACGGAGGTAGGAAACTCAGGAGCTTATGCTGGAATCTTTATGTCTAATGAGTCAGATTTCAACCTCCAAGACTTCACCATTCCCGCTCTGGTTCTCCCAATTGAGAAAGGGGTTTTGATCAAGGAGTATGCAACAAGAGCCAACAAAACAACTCAAGTTAAAACCCTGAGCTTTGTGCACACCAGTTTGGGTTCAAAACCAGCACCACAAGTGTACTCTCAATCTTCAAGAGGACCGGACCGCATCAATCCAAGCATCCTAAAACCAGACATTCTCGCTCCAGGGGTTGACGTGCTAGGTGCAGTTGCACCTAACAGGCCATCAATCTTCCACCTAAATAACTACGAGCTGGTGTCAGATTATGCACTCTTGTCAGGGACCTCAATGGCAGCACCGCACGTTGCAGGTGTTGCTGCTCTGGTAAAAGCAGTTCATAGAGAATGGAGCCCAGCGGCAATTCGATCGGCAATCATGACCAGTGCATACACTGTTGACAACACCAATGCGACCATAAAAGACCAAGAGACTAGTCTTCCAGCCACCCCTTTGGACTTCGGTGCAGGCCACATCAACCCGAATAAAGCCATGAACCCTGGATTAATCTATGACATGGATGTCCAAGATTACATTGAGTTCCTGTGTGGCCTAGGGTACAATGCAAAGCAGATGCGTGCTGTTCTTCGGCGAAGTGAGTGGAGTTGCAGTTCACATCTGACTCAGCTAAACTATCCTTCTTTTATAGCTTCCTTCAAGAGCAGCATAGACCGGTCATCTACAAAATCTGAAAATTTCACTAGGGTGGTGACAAATGTGGGAAATCATACATCGATTTACAAAGCAGTGTTGCAAGTTCCAAGAGGCCTGAAGATTGCAGTAGAGCCAAGTACTCTAGCATTCTCCAGAAAGAATCAGCAGCATGCTTTCTCTCTGAGGGTAGAGATTGACGGAAATGCTTCGAGAGTGACATACGGCTACCTGAAGTGGATTGATCAACATAGCCACATTGTATCAAGCCCCGTAGTGATTGTTACTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

519

Amino Acids

56.11

Weight (kDa)

6.64

Isoelectric Point (pI)

34.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	1 - 340	3e-30	Subtilase family
fn3_6	PF17766	413 - 515	1.7e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002861)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34120 FvH4_2g34130 FvH4_2g34150 FvH4_2g34210 FvH4_2g34211 FvH4_2g34212
malus_domestica	MD05G1275700.v1.1 MD05G1275800.v1.1 MD08G1098000.v1.1 MD15G1080200.v1.1 MD15G1080300.v1.1
prunus_persica	Prupe.1G433100_v2.0.a1 Prupe.1G433200_v2.0.a1
pyrus_communis	pycom15g07460
rosa_chinensis	RchiOBHm_Chr6g0308201 RchiOBHm_Chr6g0308211 RchiOBHm_Chr6g0308221
rosa_laevigata	RLG00000010657 RLG00000010658 RLG00000010659
rosa_multiflora	Rmu_sc0005706.1_g000017 Rmu_sc0005706.1_g000018 Rmu_ssc0000355.1_g000001 Rmu_ssc0000355.1_g000006
rosa_roxburghii	Rroxscaffold_7G00160340 Rroxscaffold_7G00160360 Rroxscaffold_7G00160440 Rroxscaffold_7G00160450
rosa_rugosa	Rorug01G0005200 Rorug01G0066300 Rorug06G0364700 Rorug06G0364800 Rorug06G0364900
rosa_samantha	Rh6DG475900 Rh6DG476100 Rh6DG476300
rosa_wichuraiana	Rw6G041330 Rw6G041340 Rw6G041350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	914
AasI	GACNNNNNNGTC	1 cut(s)	1133
Acc36I	ACCTGC	1 cut(s)	914
AccB1I	GGYRCC	2 cut(s)	4, 281
AccBSI	CCGCTC	1 cut(s)	605
AccII	CGCG	1 cut(s)	10
AciI	CCGC	8 cut(s)	8, 233, 342, 365, 603, 760, 914, 971
AclWI	GGATC	2 cut(s)	275, 303
AcsI	RAATTY	2 cut(s)	314, 1306
AcuI	CTGAAG	3 cut(s)	366, 1403, 1517
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	5 cut(s)	29, 301, 734, 1172, 1405
AgeI	ACCGGT	1 cut(s)	1284
AgsI	TTSAA	6 cut(s)	154, 239, 580, 714, 747, 1270
AhlI	ACTAGT	1 cut(s)	1046
AjiI	CACGTC	1 cut(s)	811
AjnI	CCWGG	3 cut(s)	436, 799, 1111
AleI	CACNNNNGTG	1 cut(s)	1316
AloI	GAACNNNNNNTCC	2 cut(s)	1207, 1239
AluBI	AGCT	8 cut(s)	62, 77, 243, 542, 690, 868, 1240, 1262
AluI	AGCT	8 cut(s)	62, 77, 243, 542, 690, 868, 1240, 1262
Alw21I	GWGCWC	2 cut(s)	16, 699
Alw26I	GTCTC	1 cut(s)	1037
Alw44I	GTGCAC	2 cut(s)	12, 695
AlwI	GGATC	2 cut(s)	275, 303
AoxI	GGCC	5 cut(s)	90, 836, 1081, 1162, 1378
ApaLI	GTGCAC	2 cut(s)	12, 695
ApeKI	GCWGC	4 cut(s)	908, 932, 1275, 1432
ApoI	RAATTY	2 cut(s)	314, 1306
AseI	ATTAAT	1 cut(s)	1118
AsiGI	ACCGGT	1 cut(s)	1284
AsiSI	GCGATCGC	1 cut(s)	171
Asp700I	GAANNNNTTC	1 cut(s)	1471
AspA2I	CCTAGG	1 cut(s)	1165
AspLEI	GCGC	1 cut(s)	7
AspS9I	GGNCC	5 cut(s)	90, 230, 752, 757, 897
AsuHPI	GGTGA	2 cut(s)	586, 1333
AsuNHI	GCTAGC	1 cut(s)	73
AvaII	GGWCC	4 cut(s)	230, 752, 757, 897
AvrII	CCTAGG	1 cut(s)	1165
BaeGI	GKGCMC	2 cut(s)	16, 699
BanI	GGYRCC	2 cut(s)	4, 281
BanII	GRGCYC	2 cut(s)	262, 968
BbsI	GAAGAC	1 cut(s)	1043
Bbv12I	GWGCWC	2 cut(s)	16, 699
BbvI	GCAGC	4 cut(s)	919, 920, 1287, 1444
BccI	CCATC	4 cut(s)	33, 95, 320, 847
BceAI	ACGGC	1 cut(s)	1504
BcgI	CGANNNNNNTGC	4 cut(s)	146, 180, 963, 997
BciT130I	CCWGG	3 cut(s)	438, 801, 1113
BclI	TGATCA	2 cut(s)	636, 1507
BcoDI	GTCTC	1 cut(s)	1037
BcuI	ACTAGT	1 cut(s)	1046
BfaI	CTAG	8 cut(s)	63, 74, 815, 1047, 1166, 1314, 1409, 1555
BfmI	CTRYAG	1 cut(s)	273
BfoI	RGCGCY	1 cut(s)	8
BfuAI	ACCTGC	1 cut(s)	914
BisI	GCNGC	6 cut(s)	8, 909, 933, 972, 1276, 1433
BlnI	CCTAGG	1 cut(s)	1165
BlpI	GCTNAGC	1 cut(s)	368
BlsI	GCNGC	6 cut(s)	9, 910, 934, 973, 1277, 1434
BmcAI	AGTACT	1 cut(s)	1405
Bme1390I	CCNGG	3 cut(s)	438, 801, 1113
Bme18I	GGWCC	4 cut(s)	230, 752, 757, 897
BmgBI	CACGTC	1 cut(s)	811
BmgT120I	GGNCC	5 cut(s)	90, 230, 752, 757, 897
BmiI	GGNNCC	5 cut(s)	6, 259, 283, 898, 965
BmrFI	CCNGG	3 cut(s)	438, 801, 1113
BmsI	GCATC	4 cut(s)	376, 771, 783, 1176
BmtI	GCTAGC	1 cut(s)	77
BpiI	GAAGAC	1 cut(s)	1043
BpmI	CTGGAG	2 cut(s)	783, 1402
Bpu10I	CCTNAGC	1 cut(s)	686
Bpu1102I	GCTNAGC	1 cut(s)	368
BpuEI	CTTGAG	1 cut(s)	657
Bsa29I	ATCGAT	1 cut(s)	1346
BsaBI	GATNNNNATC	1 cut(s)	167
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	6 cut(s)	263, 436, 437, 800, 1111, 1165
BsaWI	WCCGGW	2 cut(s)	754, 1284
BsaXI	ACNNNNNCTCC	2 cut(s)	1207, 1237
Bse118I	RCCGGY	1 cut(s)	1284
Bse1I	ACTGG	2 cut(s)	702, 996
Bse8I	GATNNNNATC	1 cut(s)	167
BseBI	CCWGG	3 cut(s)	438, 801, 1113
BseCI	ATCGAT	1 cut(s)	1346
BseDI	CCNNGG	6 cut(s)	263, 436, 437, 800, 1111, 1165
BseGI	GGATG	4 cut(s)	44, 248, 774, 1138
BseJI	GATNNNNATC	1 cut(s)	167
BseMII	CTCAG	5 cut(s)	195, 549, 677, 1250, 1439
BseNI	ACTGG	2 cut(s)	702, 996
BseSI	GKGCMC	2 cut(s)	16, 699
BseXI	GCAGC	4 cut(s)	919, 920, 1287, 1444
BseYI	CCCAGC	1 cut(s)	967
BsgI	GTGCAG	2 cut(s)	840, 1098
Bsh1236I	CGCG	1 cut(s)	10
Bsh1285I	CGRYCG	2 cut(s)	171, 983
BshFI	GGCC	5 cut(s)	92, 838, 1083, 1164, 1380
BshNI	GGYRCC	2 cut(s)	4, 281
BshTI	ACCGGT	1 cut(s)	1284
BshVI	ATCGAT	1 cut(s)	1346
BsiEI	CGRYCG	2 cut(s)	171, 983
BsiHKAI	GWGCWC	2 cut(s)	16, 699
BsiSI	CCGG	3 cut(s)	285, 755, 1285
BslFI	GGGAC	2 cut(s)	103, 910
BsmAI	GTCTC	1 cut(s)	1037
BsmFI	GGGAC	2 cut(s)	103, 910
BsmI	GAATGC	1 cut(s)	1412
BsnI	GGCC	5 cut(s)	92, 838, 1083, 1164, 1380
Bsp1286I	GDGCHC	4 cut(s)	16, 262, 699, 968
Bsp1407I	TGTACA	1 cut(s)	27
Bsp143I	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
Bsp1720I	GCTNAGC	1 cut(s)	368
BspACI	CCGC	8 cut(s)	8, 233, 342, 365, 603, 760, 914, 971
BspANI	GGCC	5 cut(s)	92, 838, 1083, 1164, 1380
BspCNI	CTCAG	5 cut(s)	194, 548, 678, 1249, 1440
BspDI	ATCGAT	1 cut(s)	1346
BspFNI	CGCG	1 cut(s)	10
BspHI	TCATGA	1 cut(s)	990
BspLI	GGNNCC	5 cut(s)	6, 259, 283, 898, 965
BspMI	ACCTGC	1 cut(s)	914
BspOI	GCTAGC	1 cut(s)	77
BspPI	GGATC	2 cut(s)	275, 303
BspT107I	GGYRCC	2 cut(s)	4, 281
BsrBI	CCGCTC	1 cut(s)	605
BsrFI	RCCGGY	1 cut(s)	1284
BsrGI	TGTACA	1 cut(s)	27
BsrI	ACTGG	2 cut(s)	702, 996
BssAI	RCCGGY	1 cut(s)	1284
BssECI	CCNNGG	6 cut(s)	263, 436, 437, 800, 1111, 1165
BssMI	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	1165
Bst2UI	CCWGG	3 cut(s)	438, 801, 1113
Bst4CI	ACNGT	3 cut(s)	230, 277, 1009
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	2 cut(s)	20, 929
BstAUI	TGTACA	1 cut(s)	27
BstC8I	GCNNGC	6 cut(s)	12, 75, 79, 1081, 1191, 1437
BstDEI	CTNAG	6 cut(s)	181, 368, 535, 686, 1236, 1448
BstDSI	CCRYGG	1 cut(s)	263
BstF5I	GGATG	4 cut(s)	44, 248, 774, 1138
BstFNI	CGCG	1 cut(s)	10
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	1 cut(s)	7
BstKTI	GATC	6 cut(s)	171, 270, 298, 639, 983, 1510
BstMAI	GTCTC	1 cut(s)	1037
BstMBI	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
BstMCI	CGRYCG	2 cut(s)	171, 983
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 20, 656, 929
BstNI	CCWGG	3 cut(s)	438, 801, 1113
BstNSI	RCATGY	1 cut(s)	1439
BstSCI	CCNGG	3 cut(s)	436, 799, 1111
BstSFI	CTRYAG	1 cut(s)	273
BstSLI	GKGCMC	2 cut(s)	16, 699
BstUI	CGCG	1 cut(s)	10
BstV1I	GCAGC	4 cut(s)	919, 920, 1287, 1444
BstV2I	GAAGAC	1 cut(s)	1043
BstXI	CCANNNNNNTGG	1 cut(s)	1066
Bsu15I	ATCGAT	1 cut(s)	1346
BsuRI	GGCC	5 cut(s)	92, 838, 1083, 1164, 1380
BsuTUI	ATCGAT	1 cut(s)	1346
BtgI	CCRYGG	1 cut(s)	263
BtrI	CACGTC	1 cut(s)	811
BtsCI	GGATG	4 cut(s)	44, 248, 774, 1138
BtsI	GCAGTG	1 cut(s)	1365
BtsIMutI	CAGTG	3 cut(s)	1003, 1005, 1365
BveI	ACCTGC	1 cut(s)	914
Cac8I	GCNNGC	6 cut(s)	12, 75, 79, 1081, 1191, 1437
CciI	TCATGA	1 cut(s)	990
CfoI	GCGC	1 cut(s)	7
Cfr10I	RCCGGY	1 cut(s)	1284
Cfr13I	GGNCC	5 cut(s)	90, 230, 752, 757, 897
ClaI	ATCGAT	1 cut(s)	1346
CpoI	CGGWCCG	2 cut(s)	230, 757
Csp6I	GTAC	5 cut(s)	28, 300, 733, 1171, 1404
CspAI	ACCGGT	1 cut(s)	1284
CspI	CGGWCCG	2 cut(s)	230, 757
CviAII	CATG	7 cut(s)	25, 82, 115, 991, 1105, 1129, 1436
CviQI	GTAC	5 cut(s)	28, 300, 733, 1171, 1404
DdeI	CTNAG	6 cut(s)	181, 368, 535, 686, 1236, 1448
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	6 cut(s)	170, 269, 297, 638, 982, 1509
DpnII	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
DrdI	GACNNNNNNGTC	1 cut(s)	1133
DseDI	GACNNNNNNGTC	1 cut(s)	1133
Eco130I	CCWWGG	1 cut(s)	1165
Eco147I	AGGCCT	1 cut(s)	1380
Eco24I	GRGCYC	2 cut(s)	262, 968
Eco32I	GATATC	1 cut(s)	112
Eco47I	GGWCC	4 cut(s)	230, 752, 757, 897
Eco57I	CTGAAG	3 cut(s)	366, 1403, 1517
EcoO109I	RGGNCCY	1 cut(s)	897
EcoRII	CCWGG	3 cut(s)	436, 799, 1111
EcoRV	GATATC	1 cut(s)	112
EcoT14I	CCWWGG	1 cut(s)	1165
EcoT38I	GRGCYC	2 cut(s)	262, 968
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	1 cut(s)	1165
FaeI	CATG	7 cut(s)	28, 85, 118, 994, 1108, 1132, 1439
FaqI	GGGAC	2 cut(s)	103, 910
FatI	CATG	7 cut(s)	24, 81, 114, 990, 1104, 1128, 1435
FauI	CCCGC	2 cut(s)	372, 610
FbaI	TGATCA	2 cut(s)	636, 1507
Fnu4HI	GCNGC	6 cut(s)	8, 909, 933, 972, 1276, 1433
FokI	GGATG	4 cut(s)	51, 235, 761, 1145
FriOI	GRGCYC	2 cut(s)	262, 968
Fsp4HI	GCNGC	6 cut(s)	8, 909, 933, 972, 1276, 1433
FspBI	CTAG	8 cut(s)	63, 74, 815, 1047, 1166, 1314, 1409, 1555
GlaI	GCGC	1 cut(s)	6
GluI	GCNGC	6 cut(s)	8, 909, 933, 972, 1276, 1433
GsaI	CCCAGC	1 cut(s)	971
GsuI	CTGGAG	2 cut(s)	783, 1402
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	5 cut(s)	92, 838, 1083, 1164, 1380
HapII	CCGG	3 cut(s)	285, 755, 1285
HhaI	GCGC	1 cut(s)	7
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	7 cut(s)	28, 85, 118, 994, 1108, 1132, 1439
Hin6I	GCGC	1 cut(s)	5
HinP1I	GCGC	1 cut(s)	5
HincII	GTYRAC	2 cut(s)	808, 1012
HindII	GTYRAC	2 cut(s)	808, 1012
HinfI	GANTC	5 cut(s)	448, 552, 569, 1234, 1426
HpaII	CCGG	3 cut(s)	285, 755, 1285
HphI	GGTGA	2 cut(s)	586, 1333
Hpy166II	GTNNAC	7 cut(s)	14, 376, 697, 733, 808, 1012, 1226
Hpy188I	TCNGA	7 cut(s)	38, 100, 574, 877, 1233, 1303, 1449
Hpy188III	TCNNGA	8 cut(s)	45, 452, 537, 747, 991, 1270, 1419, 1476
Hpy8I	GTNNAC	7 cut(s)	14, 376, 697, 733, 808, 1012, 1226
HpyAV	CCTTC	4 cut(s)	341, 514, 1260, 1276
HpyCH4III	ACNGT	3 cut(s)	230, 277, 1009
HpyCH4IV	ACGT	2 cut(s)	810, 918
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 20, 656, 929
HpyF3I	CTNAG	6 cut(s)	181, 368, 535, 686, 1236, 1448
HpySE526I	ACGT	2 cut(s)	810, 918
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	7 cut(s)	28, 85, 118, 994, 1108, 1132, 1439
HspAI	GCGC	1 cut(s)	5
KasI	GGCGCC	1 cut(s)	4
Ksp22I	TGATCA	2 cut(s)	636, 1507
Kzo9I	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
LmnI	GCTCC	5 cut(s)	257, 489, 539, 802, 963
Lsp1109I	GCAGC	4 cut(s)	919, 920, 1287, 1444
LweI	GCATC	4 cut(s)	376, 771, 783, 1176
MaeI	CTAG	8 cut(s)	63, 74, 815, 1047, 1166, 1314, 1409, 1555
MaeII	ACGT	2 cut(s)	810, 918
MaeIII	GTNAC	4 cut(s)	431, 1321, 1480, 1546
MalI	GATC	6 cut(s)	170, 269, 297, 638, 982, 1509
MbiI	CCGCTC	1 cut(s)	605
MboI	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
MboII	GAAGA	6 cut(s)	467, 735, 838, 1043, 1190, 1396
MfeI	CAATTG	1 cut(s)	618
MhlI	GDGCHC	4 cut(s)	16, 262, 699, 968
MluCI	AATT	5 cut(s)	198, 314, 618, 975, 1306
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	578, 1228
MmeI	TCCRAC	1 cut(s)	276
MnlI	CCTC	7 cut(s)	188, 517, 593, 743, 910, 1370, 1443
MroXI	GAANNNNTTC	1 cut(s)	1471
MseI	TTAA	2 cut(s)	678, 1118
MslI	CAYNNNNRTG	1 cut(s)	1316
MspA1I	CMGCKG	2 cut(s)	344, 971
MspI	CCGG	3 cut(s)	285, 755, 1285
MspR9I	CCNGG	3 cut(s)	438, 801, 1113
MunI	CAATTG	1 cut(s)	618
Mva1269I	GAATGC	1 cut(s)	1412
MvaI	CCWGG	3 cut(s)	438, 801, 1113
MvnI	CGCG	1 cut(s)	10
MwoI	GCNNNNNNNGC	4 cut(s)	11, 20, 656, 929
NarI	GGCGCC	1 cut(s)	5
NdeII	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
NheI	GCTAGC	1 cut(s)	73
NlaIII	CATG	7 cut(s)	28, 85, 118, 994, 1108, 1132, 1439
NlaIV	GGNNCC	5 cut(s)	6, 259, 283, 898, 965
NmuCI	GTSAC	3 cut(s)	431, 1321, 1480
NspI	RCATGY	1 cut(s)	1439
OliI	CACNNNNGTG	1 cut(s)	1316
PaeI	GCATGC	1 cut(s)	1439
PagI	TCATGA	1 cut(s)	990
PaqCI	CACCTGC	1 cut(s)	914
PasI	CCCWGGG	1 cut(s)	437
PceI	AGGCCT	1 cut(s)	1380
PctI	GAATGC	1 cut(s)	1412
PdmI	GAANNNNTTC	1 cut(s)	1471
PfeI	GAWTC	3 cut(s)	448, 552, 1426
PinAI	ACCGGT	1 cut(s)	1284
PkrI	GCNGC	6 cut(s)	9, 910, 934, 973, 1277, 1434
Ple19I	CGATCG	2 cut(s)	171, 983
PleI	GAGTC	2 cut(s)	577, 1228
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	577, 1228
PpuMI	RGGWCCY	1 cut(s)	897
PshBI	ATTAAT	1 cut(s)	1118
Psp5II	RGGWCCY	1 cut(s)	897
Psp6I	CCWGG	3 cut(s)	436, 799, 1111
PspFI	CCCAGC	1 cut(s)	967
PspGI	CCWGG	3 cut(s)	436, 799, 1111
PspN4I	GGNNCC	5 cut(s)	6, 259, 283, 898, 965
PspPI	GGNCC	5 cut(s)	90, 230, 752, 757, 897
PspPPI	RGGWCCY	1 cut(s)	897
PsrI	GAACNNNNNNTAC	2 cut(s)	933, 965
PvuI	CGATCG	2 cut(s)	171, 983
RgaI	GCGATCGC	1 cut(s)	171
RsaI	GTAC	5 cut(s)	29, 301, 734, 1172, 1405
RsaNI	GTAC	5 cut(s)	28, 300, 733, 1171, 1404
RseI	CAYNNNNRTG	1 cut(s)	1316
Rsr2I	CGGWCCG	2 cut(s)	230, 757
RsrII	CGGWCCG	2 cut(s)	230, 757
SaqAI	TTAA	2 cut(s)	678, 1118
SatI	GCNGC	6 cut(s)	8, 909, 933, 972, 1276, 1433
Sau3AI	GATC	6 cut(s)	168, 267, 295, 636, 980, 1507
Sau96I	GGNCC	5 cut(s)	90, 230, 752, 757, 897
ScaI	AGTACT	1 cut(s)	1405
SchI	GAGTC	2 cut(s)	578, 1228
ScrFI	CCNGG	3 cut(s)	438, 801, 1113
SduI	GDGCHC	4 cut(s)	16, 262, 699, 968
SfaAI	GCGATCGC	1 cut(s)	171
SfaNI	GCATC	4 cut(s)	376, 771, 783, 1176
SfcI	CTRYAG	1 cut(s)	273
SfoI	GGCGCC	1 cut(s)	6
SgfI	GCGATCGC	1 cut(s)	171
SinI	GGWCC	4 cut(s)	230, 752, 757, 897
SmiMI	CAYNNNNRTG	1 cut(s)	1316
SmlI	CTYRAG	1 cut(s)	672
SmoI	CTYRAG	1 cut(s)	672
SpeI	ACTAGT	1 cut(s)	1046
SphI	GCATGC	1 cut(s)	1439
Sse9I	AATT	5 cut(s)	198, 314, 618, 975, 1306
SseBI	AGGCCT	1 cut(s)	1380
SsiI	CCGC	8 cut(s)	8, 233, 342, 365, 603, 760, 914, 971
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	1 cut(s)	149
SspMI	CTAG	8 cut(s)	63, 74, 815, 1047, 1166, 1314, 1409, 1555
StuI	AGGCCT	1 cut(s)	1380
StyD4I	CCNGG	3 cut(s)	436, 799, 1111
StyI	CCWWGG	1 cut(s)	1165
TaaI	ACNGT	3 cut(s)	230, 277, 1009
TaiI	ACGT	2 cut(s)	813, 921
TaqI	TCGA	4 cut(s)	324, 979, 1346, 1475
TasI	AATT	5 cut(s)	198, 314, 618, 975, 1306
TatI	WGTACW	3 cut(s)	27, 732, 1403
TauI	GCSGC	2 cut(s)	10, 974
TfiI	GAWTC	3 cut(s)	448, 552, 1426
Tru1I	TTAA	2 cut(s)	678, 1118
Tru9I	TTAA	2 cut(s)	678, 1118
TscAI	CASTG	3 cut(s)	1003, 1012, 1365
TseFI	GTSAC	3 cut(s)	431, 1321, 1480
TseI	GCWGC	4 cut(s)	908, 932, 1275, 1432
Tsp45I	GTSAC	3 cut(s)	431, 1321, 1480
TspDTI	ATGAA	2 cut(s)	941, 1121
TspGWI	ACGGA	3 cut(s)	270, 536, 1479
TspRI	CASTG	3 cut(s)	1003, 1012, 1365
VneI	GTGCAC	2 cut(s)	12, 695
VpaK11BI	GGWCC	4 cut(s)	230, 752, 757, 897
VspI	ATTAAT	1 cut(s)	1118
XapI	RAATTY	2 cut(s)	314, 1306
XceI	RCATGY	1 cut(s)	1439
XcmI	CCANNNNNNNNNTGG	1 cut(s)	604
XmaJI	CCTAGG	1 cut(s)	1165
XmnI	GAANNNNTTC	1 cut(s)	1471
XspI	CTAG	8 cut(s)	63, 74, 815, 1047, 1166, 1314, 1409, 1555
ZrmI	AGTACT	1 cut(s)	1405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.