Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002861

Physical Location & Seq

Genomic Coordinates Forward (+)

65053378 .. 65055663

Gene Structure 2286 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G041340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGAGTTTCTACCTTATGATCTCTTTATTAGTTTGTACGCTACTGCAAATCACCAATGCAGTTTCAAGTGCTGAGGAATCCCAAACATACATCATTCACATGGATCATTCCCAAAAACCTTCGTCTTTTTTGACTCACGAGGCATGGCACAGGTCTACCTTAACTTCGTTAATGTCATCTTCGTCTCTAGCAGATCCAGAGAACGAAATGTTGTTGTACTCATACAACCATGTCATGCACGGTTTCAGTGCCAGGCTTACAGCTTCTCAACTGCCCGAATTGGAGGAATCTCCGGCTCATGTTGCCACGTATCCGGAGTCTTTTGGTAAGCTCTTGACAACACACAGCTCCAAGTTTCTAGGACTGAGACGGAACTCAGGCTTATTGCTTGCTTCCTCGTCTGGCCAAGATGCGATCATAGGAATCATAGATACCGGAGTTTGGCCAGAGAGCGAGAGTTTCGGTGATAGGGGAATGTCTGAAGTGCCAAAAAGATGGAAAGGCACTTGCGAGAATGGAACATATTTCACCCCATCTCTCTGCAACAACAAGCTCATTGGGGCTCGATCCTTTAGCAAAGGCCTGAAAGCAGCAGGAATTAAAATCTCCACAAAAAATGACTTCGATTCACCATGGGACTGGTTTGGTCATGGAAGTCACACTTCGTCAACAGCTGCAGGTAACCATGTACCCGGCGCAAGCCATTTCGGATATGCAAAAGGCACGGCAAGAGGGGTGGCGCCACGAGCCCATGTTGCCATGTACAAGGTCGCCTGGGCAACAGACTCGGAAAAGAGTTTCGCTAGTGATGTGCTTGCAGGCATGGACCAAGCAATTTCTGATGGTGTTGATATAATGTCACTGTCTCTTGCCTTTGACTTCCTTCCTTATTTCAACGATGTCATTGCCATTGCTTCTCTTTCTGCAATCGAACAAGGAATTGTTGTGGTCTGTGCTGCTGGAAATGACGGGAAATACAGTACAACATACAATGGAGCACCCTGGATCACAACAGTAGGAGCCGGAACTCTTGATCGCACCTTCACTGCAACACTGACTCTAGATAATGGTTTGACGGTTGAAGCAACATCGTACTTCCCCGAGAGCGTTTACATTACTGATGTGCCATTGTACTACGGCAAAGACAATGCAACGAAAGCAATATGCAATACTGGGTCACTGGATCACAACGAAGTGGCAGGAAAGGTAGTCTTTTGTGATAATACCACTGAGACTCCCGTCAATGGAGTCGACTTCCAAAAGAAGGAGGTGGATAGGGCAGGTGGTTATGCTGGAATCTTCATGGCAGATCTCTCACTTTTAGAGCCAGAAGACTTCTCCATTCCTGCTATCATTGTTCCATTTGCTACAGGGGCCTTGATTAGAGAGTATGCAACGCAGGTCCATACACCTAGAGTTAAGATCCTGAGTTTTGTTCACACAAATTTGGGTACAAAACCATCACCGGAAGTAGCGTATTTCTCTTCAAGGGGACCAGACCCAATCACTCCTGGTATTCTAAAACCAGACATTCTTGCTCCAGGAGTCGATGTGCTTGCTGCAGTTACACCTAACAACCCATGCATGGAAGTAAACAATTATAATCTAGTGACAGATTATGAACTATATTCAGGAACATCAATGGCGGCACCCCATGCCGCTGGTGTGGCAGCTCTGCTAAAGGCTGTTCACCGAAAATGGAGTCCAGCAGCCATCCGATCTGCCATCATGACCACAGCATACTCAACCACCAATTCTCACACCAGCATACAACACCAATGGTCCGGTCTTCCAGCCACACCTTTAGATTTTGGTGCAGGCCACATCAACCCGAATAAGGCCATGAACCCCGGACTAGTCTATGACATGAATGTGCAAGACTACATTGATTTTCTTTGTACCCTTGGGTACAATGACAAGCAGATGAAAGCTGTTATTAGACGAAGCCAGTGGACTTGCAGACAGCCCCCTACCGAACTAAACTACCCCTCATTCGTGGCCATCTTCAACAAAACAGACTCTTCAAAAGCCAAAAACTTTAGCAGGGTTGTGACAAACGTGGGGGATGATACCTCCATCTACCGAGCATTTCTAGAAGTTCCTAGAGGAATGAGGATTAAAGTAGAGCCTAATTCTTTTACATTCACCGGAAAATATCAGCAGCAAGGGTTTGTTTTGAATGTAGAGATCGATAGCGATGCTCCTAAGGTGATCTACGGTTATCTCAGATGGGTTGATCACCATGATCACATGGTATCAAGCCCTGTAGTGGCCATTAATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

761

Amino Acids

82.76

Weight (kDa)

5.88

Isoelectric Point (pI)

37.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	29 - 107	7.5e-17	Peptidase inhibitor I9
Peptidase_S8	PF00082	135 - 584	8e-41	Subtilase family
fn3_6	PF17766	659 - 757	3.3e-23	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002861)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34120 FvH4_2g34130 FvH4_2g34150 FvH4_2g34210 FvH4_2g34211 FvH4_2g34212
malus_domestica	MD05G1275700.v1.1 MD05G1275800.v1.1 MD08G1098000.v1.1 MD15G1080200.v1.1 MD15G1080300.v1.1
prunus_persica	Prupe.1G433100_v2.0.a1 Prupe.1G433200_v2.0.a1
pyrus_communis	pycom15g07460
rosa_chinensis	RchiOBHm_Chr6g0308201 RchiOBHm_Chr6g0308211 RchiOBHm_Chr6g0308221
rosa_laevigata	RLG00000010657 RLG00000010658 RLG00000010659
rosa_multiflora	Rmu_sc0005706.1_g000017 Rmu_sc0005706.1_g000018 Rmu_ssc0000355.1_g000001 Rmu_ssc0000355.1_g000006
rosa_roxburghii	Rroxscaffold_7G00160340 Rroxscaffold_7G00160360 Rroxscaffold_7G00160440 Rroxscaffold_7G00160450
rosa_rugosa	Rorug01G0005200 Rorug01G0066300 Rorug06G0364700 Rorug06G0364800 Rorug06G0364900
rosa_samantha	Rh6DG475900 Rh6DG476100 Rh6DG476300
rosa_wichuraiana	Rw6G041330 Rw6G041340 Rw6G041350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1602
AarI	CACCTGC	1 cut(s)	1271
Acc36I	ACCTGC	3 cut(s)	668, 1271, 1390
AccB1I	GGYRCC	2 cut(s)	739, 1648
AccI	GTMKAC	2 cut(s)	155, 1251
AccIII	TCCGGA	1 cut(s)	313
AciI	CCGC	2 cut(s)	1646, 1659
AclWI	GGATC	6 cut(s)	111, 188, 561, 1013, 1191, 1417
AcoI	YGGCCR	4 cut(s)	403, 443, 1998, 2271
AcsI	RAATTY	1 cut(s)	1444
AcuI	CTGAAG	1 cut(s)	501
AcyI	GRCGYC	1 cut(s)	740
AfiI	CCNNNNNNNGG	6 cut(s)	441, 1244, 1264, 1585, 1837, 2269
AgsI	TTSAA	7 cut(s)	66, 895, 1082, 1488, 2008, 2025, 2179
AhlI	ACTAGT	1 cut(s)	1855
AjnI	CCWGG	5 cut(s)	251, 773, 1001, 1510, 1540
AluBI	AGCT	7 cut(s)	263, 331, 348, 553, 674, 1673, 1931
AluI	AGCT	7 cut(s)	263, 331, 348, 553, 674, 1673, 1931
Alw21I	GWGCWC	1 cut(s)	1000
Alw26I	GTCTC	4 cut(s)	189, 361, 870, 1226
AlwI	GGATC	6 cut(s)	111, 188, 561, 1013, 1191, 1417
Ama87I	CYCGRG	1 cut(s)	1100
Aor13HI	TCCGGA	1 cut(s)	313
AoxI	GGCC	8 cut(s)	403, 443, 580, 1374, 1819, 1839, 1998, 2271
ApeKI	GCWGC	7 cut(s)	590, 674, 956, 1559, 1670, 1709, 2161
ApoI	RAATTY	1 cut(s)	1444
ArsI	GACNNNNNNTTYG	2 cut(s)	1687, 1719
AseI	ATTAAT	1 cut(s)	2277
AspLEI	GCGC	2 cut(s)	698, 742
AspS9I	GGNCC	5 cut(s)	826, 1374, 1402, 1493, 1782
AsuC2I	CCSGG	2 cut(s)	693, 1851
AsuHPI	GGTGA	9 cut(s)	43, 476, 520, 621, 1455, 1682, 2137, 2221, 2231
AvaI	CYCGRG	1 cut(s)	1100
AvaII	GGWCC	4 cut(s)	826, 1402, 1493, 1782
AxyI	CCTNAGG	1 cut(s)	2205
BaeI	ACNNNNGTAYC	2 cut(s)	423, 456
BalI	TGGCCA	4 cut(s)	405, 445, 2000, 2273
BanI	GGYRCC	2 cut(s)	739, 1648
BanII	GRGCYC	2 cut(s)	565, 751
BauI	CACGAG	2 cut(s)	137, 744
BbsI	GAAGAC	2 cut(s)	1338, 1781
Bbv12I	GWGCWC	1 cut(s)	1000
BbvCI	CCTCAGC	1 cut(s)	72
BbvI	GCAGC	7 cut(s)	602, 661, 943, 1546, 1682, 1721, 2173
BccI	CCATC	9 cut(s)	489, 541, 836, 1468, 1721, 1733, 2009, 2084, 2223
BceAI	ACGGC	2 cut(s)	741, 1153
BcgI	CGANNNNNNTGC	8 cut(s)	768, 802, 887, 921, 1538, 1572, 2180, 2214
BciT130I	CCWGG	5 cut(s)	253, 775, 1003, 1512, 1542
BciVI	GTATCC	1 cut(s)	321
BclI	TGATCA	2 cut(s)	2236, 2245
BcnI	CCSGG	2 cut(s)	693, 1851
BcoDI	GTCTC	4 cut(s)	189, 361, 870, 1226
BcuI	ACTAGT	1 cut(s)	1855
BfmI	CTRYAG	4 cut(s)	675, 1368, 1560, 2265
BfoI	RGCGCY	1 cut(s)	743
BfuAI	ACCTGC	3 cut(s)	668, 1271, 1390
BfuI	GTATCC	1 cut(s)	321
BglII	AGATCT	1 cut(s)	1309
BisI	GCNGC	9 cut(s)	591, 675, 957, 1560, 1647, 1659, 1671, 1710, 2162
BlsI	GCNGC	9 cut(s)	592, 676, 958, 1561, 1648, 1660, 1672, 1711, 2163
Bme1390I	CCNGG	7 cut(s)	253, 693, 775, 1003, 1512, 1542, 1851
Bme18I	GGWCC	4 cut(s)	826, 1402, 1493, 1782
BmeT110I	CYCGRG	1 cut(s)	1100
BmgT120I	GGNCC	5 cut(s)	826, 1374, 1402, 1493, 1782
BmiI	GGNNCC	5 cut(s)	741, 1021, 1375, 1494, 1650
BmrFI	CCNGG	7 cut(s)	253, 693, 775, 1003, 1512, 1542, 1851
BmrI	ACTGGG	1 cut(s)	1182
BmsI	GCATC	2 cut(s)	400, 2188
BmuI	ACTGGG	1 cut(s)	1182
BoxI	GACNNNNGTC	1 cut(s)	1238
BpiI	GAAGAC	2 cut(s)	1338, 1781
BpmI	CTGGAG	1 cut(s)	1524
Bpu10I	CCTNAGC	1 cut(s)	72
BpuMI	CCSGG	2 cut(s)	693, 1851
Bsa29I	ATCGAT	1 cut(s)	2190
BsaAI	YACGTR	1 cut(s)	309
BsaHI	GRCGYC	1 cut(s)	740
BsaJI	CCNNGG	5 cut(s)	632, 774, 1001, 1849, 1903
BsaWI	WCCGGW	5 cut(s)	313, 434, 1465, 1784, 2147
BsaXI	ACNNNNNCTCC	4 cut(s)	1219, 1249, 1536, 1566
Bsc4I	CCNNNNNNNGG	6 cut(s)	441, 1244, 1264, 1585, 1837, 2269
Bse1I	ACTGG	4 cut(s)	644, 1177, 1185, 1948
Bse21I	CCTNAGG	1 cut(s)	2205
Bse3DI	GCAATG	2 cut(s)	903, 909
BseAI	TCCGGA	1 cut(s)	313
BseBI	CCWGG	5 cut(s)	253, 775, 1003, 1512, 1542
BseCI	ATCGAT	1 cut(s)	2190
BseDI	CCNNGG	5 cut(s)	632, 774, 1001, 1849, 1903
BseGI	GGATG	2 cut(s)	1713, 2071
BseLI	CCNNNNNNNGG	6 cut(s)	441, 1244, 1264, 1585, 1837, 2269
BseMI	GCAATG	2 cut(s)	903, 909
BseMII	CTCAG	6 cut(s)	63, 356, 390, 1221, 1418, 2239
BseNI	ACTGG	4 cut(s)	644, 1177, 1185, 1948
BseXI	GCAGC	7 cut(s)	602, 661, 943, 1546, 1682, 1721, 2173
BsgI	GTGCAG	1 cut(s)	1836
BshFI	GGCC	8 cut(s)	405, 445, 582, 1376, 1821, 1841, 2000, 2273
BshNI	GGYRCC	2 cut(s)	739, 1648
BshVI	ATCGAT	1 cut(s)	2190
BsiHKAI	GWGCWC	1 cut(s)	1000
BsiHKCI	CYCGRG	1 cut(s)	1100
BsiSI	CCGG	9 cut(s)	293, 314, 435, 693, 1023, 1466, 1785, 1851, 2148
BslFI	GGGAC	2 cut(s)	650, 1506
BslI	CCNNNNNNNGG	6 cut(s)	441, 1244, 1264, 1585, 1837, 2269
BsmAI	GTCTC	4 cut(s)	189, 361, 870, 1226
BsmBI	CGTCTC	2 cut(s)	189, 361
BsmFI	GGGAC	2 cut(s)	650, 1506
BsnI	GGCC	8 cut(s)	405, 445, 582, 1376, 1821, 1841, 2000, 2273
BsoBI	CYCGRG	1 cut(s)	1100
Bsp1286I	GDGCHC	3 cut(s)	565, 751, 1000
Bsp13I	TCCGGA	1 cut(s)	313
Bsp1407I	TGTACA	1 cut(s)	762
Bsp19I	CCATGG	1 cut(s)	632
BspACI	CCGC	2 cut(s)	1646, 1659
BspANI	GGCC	8 cut(s)	405, 445, 582, 1376, 1821, 1841, 2000, 2273
BspCNI	CTCAG	6 cut(s)	64, 357, 389, 1222, 1419, 2238
BspDI	ATCGAT	1 cut(s)	2190
BspEI	TCCGGA	1 cut(s)	313
BspHI	TCATGA	1 cut(s)	1728
BspLI	GGNNCC	5 cut(s)	741, 1021, 1375, 1494, 1650
BspMAI	CTGCAG	2 cut(s)	679, 1564
BspMI	ACCTGC	3 cut(s)	668, 1271, 1390
BspPI	GGATC	6 cut(s)	111, 188, 561, 1013, 1191, 1417
BspT107I	GGYRCC	2 cut(s)	739, 1648
BsrDI	GCAATG	2 cut(s)	903, 909
BsrGI	TGTACA	1 cut(s)	762
BsrI	ACTGG	4 cut(s)	644, 1177, 1185, 1948
BssECI	CCNNGG	5 cut(s)	632, 774, 1001, 1849, 1903
BssNI	GRCGYC	1 cut(s)	740
BssSI	CACGAG	2 cut(s)	137, 744
BssT1I	CCWWGG	2 cut(s)	632, 1903
Bst2BI	CACGAG	2 cut(s)	137, 744
Bst2UI	CCWGG	5 cut(s)	253, 775, 1003, 1512, 1542
Bst4CI	ACNGT	6 cut(s)	242, 864, 980, 1015, 1078, 2219
Bst6I	CTCTTC	2 cut(s)	1489, 2026
BstACI	GRCGYC	1 cut(s)	740
BstAPI	GCANNNNNTGC	1 cut(s)	1655
BstAUI	TGTACA	1 cut(s)	762
BstBAI	YACGTR	1 cut(s)	309
BstC8I	GCNNGC	6 cut(s)	390, 700, 816, 820, 1557, 1819
BstDEI	CTNAG	7 cut(s)	72, 365, 376, 1230, 1427, 2205, 2225
BstDSI	CCRYGG	1 cut(s)	632
BstEII	GGTNACC	1 cut(s)	680
BstF5I	GGATG	2 cut(s)	1713, 2071
BstH2I	RGCGCY	1 cut(s)	743
BstHHI	GCGC	2 cut(s)	698, 742
BstMAI	GTCTC	4 cut(s)	189, 361, 870, 1226
BstMWI	GCNNNNNNNGC	7 cut(s)	302, 746, 755, 1157, 1373, 1655, 1667
BstNI	CCWGG	5 cut(s)	253, 775, 1003, 1512, 1542
BstPAI	GACNNNNGTC	1 cut(s)	1238
BstPI	GGTNACC	1 cut(s)	680
BstSCI	CCNGG	7 cut(s)	251, 691, 773, 1001, 1510, 1540, 1849
BstSFI	CTRYAG	4 cut(s)	675, 1368, 1560, 2265
BstV1I	GCAGC	7 cut(s)	602, 661, 943, 1546, 1682, 1721, 2173
BstV2I	GAAGAC	2 cut(s)	1338, 1781
BstX2I	RGATCY	3 cut(s)	193, 1309, 1422
BstXI	CCANNNNNNTGG	3 cut(s)	495, 639, 1661
BstYI	RGATCY	3 cut(s)	193, 1309, 1422
Bsu15I	ATCGAT	1 cut(s)	2190
Bsu36I	CCTNAGG	1 cut(s)	2205
BsuI	GTATCC	1 cut(s)	321
BsuRI	GGCC	8 cut(s)	405, 445, 582, 1376, 1821, 1841, 2000, 2273
BsuTUI	ATCGAT	1 cut(s)	2190
BtgI	CCRYGG	1 cut(s)	632
BtgZI	GCGATG	1 cut(s)	2211
BtsCI	GGATG	2 cut(s)	1713, 2071
BtsI	GCAGTG	1 cut(s)	1044
BtsIMutI	CAGTG	7 cut(s)	253, 860, 1044, 1052, 1178, 1227, 1955
BveI	ACCTGC	3 cut(s)	668, 1271, 1390
Cac8I	GCNNGC	6 cut(s)	390, 700, 816, 820, 1557, 1819
CciI	TCATGA	1 cut(s)	1728
CfoI	GCGC	2 cut(s)	698, 742
Cfr13I	GGNCC	5 cut(s)	826, 1374, 1402, 1493, 1782
ClaI	ATCGAT	1 cut(s)	2190
CspCI	CAANNNNNGTGG	2 cut(s)	717, 752
DdeI	CTNAG	7 cut(s)	72, 365, 376, 1230, 1427, 2205, 2225
DinI	GGCGCC	1 cut(s)	741
EaeI	YGGCCR	4 cut(s)	403, 443, 1998, 2271
Eam1104I	CTCTTC	2 cut(s)	1489, 2026
EarI	CTCTTC	2 cut(s)	1489, 2026
Eco130I	CCWWGG	2 cut(s)	632, 1903
Eco147I	AGGCCT	1 cut(s)	582
Eco24I	GRGCYC	2 cut(s)	565, 751
Eco47I	GGWCC	4 cut(s)	826, 1402, 1493, 1782
Eco57I	CTGAAG	1 cut(s)	501
Eco81I	CCTNAGG	1 cut(s)	2205
Eco88I	CYCGRG	1 cut(s)	1100
Eco91I	GGTNACC	1 cut(s)	680
EcoO109I	RGGNCCY	1 cut(s)	1374
EcoO65I	GGTNACC	1 cut(s)	680
EcoRII	CCWGG	5 cut(s)	251, 773, 1001, 1510, 1540
EcoT14I	CCWWGG	2 cut(s)	632, 1903
EcoT22I	ATGCAT	1 cut(s)	1586
EcoT38I	GRGCYC	2 cut(s)	565, 751
EgeI	GGCGCC	1 cut(s)	741
EheI	GGCGCC	1 cut(s)	741
ErhI	CCWWGG	2 cut(s)	632, 1903
Esp3I	CGTCTC	2 cut(s)	189, 361
FalI	AAGNNNNNCTT	4 cut(s)	646, 678, 1196, 1228
FaqI	GGGAC	2 cut(s)	650, 1506
FbaI	TGATCA	2 cut(s)	2236, 2245
FblI	GTMKAC	2 cut(s)	155, 1251
Fnu4HI	GCNGC	9 cut(s)	591, 675, 957, 1560, 1647, 1659, 1671, 1710, 2162
FokI	GGATG	2 cut(s)	1700, 2078
FriOI	GRGCYC	2 cut(s)	565, 751
Fsp4HI	GCNGC	9 cut(s)	591, 675, 957, 1560, 1647, 1659, 1671, 1710, 2162
GlaI	GCGC	2 cut(s)	697, 741
GluI	GCNGC	9 cut(s)	591, 675, 957, 1560, 1647, 1659, 1671, 1710, 2162
GsuI	CTGGAG	1 cut(s)	1524
HaeII	RGCGCY	1 cut(s)	743
HaeIII	GGCC	8 cut(s)	405, 445, 582, 1376, 1821, 1841, 2000, 2273
HapII	CCGG	9 cut(s)	293, 314, 435, 693, 1023, 1466, 1785, 1851, 2148
HhaI	GCGC	2 cut(s)	698, 742
Hin1I	GRCGYC	1 cut(s)	740
Hin6I	GCGC	2 cut(s)	696, 740
HinP1I	GCGC	2 cut(s)	696, 740
HincII	GTYRAC	2 cut(s)	669, 1252
HindII	GTYRAC	2 cut(s)	669, 1252
HpaII	CCGG	9 cut(s)	293, 314, 435, 693, 1023, 1466, 1785, 1851, 2148
HphI	GGTGA	9 cut(s)	43, 476, 520, 621, 1455, 1682, 2137, 2221, 2231
Hpy166II	GTNNAC	8 cut(s)	156, 669, 1111, 1252, 1438, 1594, 1690, 1953
Hpy188I	TCNGA	6 cut(s)	481, 710, 790, 841, 1718, 2228
Hpy8I	GTNNAC	8 cut(s)	156, 669, 1111, 1252, 1438, 1594, 1690, 1953
HpyAV	CCTTC	4 cut(s)	129, 893, 1051, 1258
HpyCH4III	ACNGT	6 cut(s)	242, 864, 980, 1015, 1078, 2219
HpyCH4IV	ACGT	2 cut(s)	308, 2058
HpyF10VI	GCNNNNNNNGC	7 cut(s)	302, 746, 755, 1157, 1373, 1655, 1667
HpyF3I	CTNAG	7 cut(s)	72, 365, 376, 1230, 1427, 2205, 2225
HpySE526I	ACGT	2 cut(s)	308, 2058
Hsp92I	GRCGYC	1 cut(s)	740
HspAI	GCGC	2 cut(s)	696, 740
KasI	GGCGCC	1 cut(s)	739
Kpn2I	TCCGGA	1 cut(s)	313
Ksp22I	TGATCA	2 cut(s)	2236, 2245
LmnI	GCTCC	5 cut(s)	353, 995, 1019, 1543, 2206
Lsp1109I	GCAGC	7 cut(s)	602, 661, 943, 1546, 1682, 1721, 2173
LweI	GCATC	2 cut(s)	400, 2188
MaeII	ACGT	2 cut(s)	308, 2058
MaeIII	GTNAC	7 cut(s)	656, 680, 858, 1176, 1564, 1609, 2050
MboII	GAAGA	7 cut(s)	171, 1291, 1343, 1476, 1781, 1996, 2013
MflI	RGATCY	3 cut(s)	193, 1309, 1422
MhlI	GDGCHC	3 cut(s)	565, 751, 1000
MlsI	TGGCCA	4 cut(s)	405, 445, 2000, 2273
MluCI	AATT	8 cut(s)	278, 597, 834, 939, 1444, 1597, 1753, 2131
MluNI	TGGCCA	4 cut(s)	405, 445, 2000, 2273
Mly113I	GGCGCC	1 cut(s)	740
MlyI	GAGTC	9 cut(s)	127, 326, 779, 1051, 1228, 1257, 1554, 1711, 2012
Mox20I	TGGCCA	4 cut(s)	405, 445, 2000, 2273
Mph1103I	ATGCAT	1 cut(s)	1586
MroI	TCCGGA	1 cut(s)	313
MscI	TGGCCA	4 cut(s)	405, 445, 2000, 2273
MseI	TTAA	6 cut(s)	161, 170, 600, 1419, 2118, 2277
MslI	CAYNNNNRTG	2 cut(s)	98, 1871
Msp20I	TGGCCA	4 cut(s)	405, 445, 2000, 2273
MspA1I	CMGCKG	2 cut(s)	674, 1661
MspI	CCGG	9 cut(s)	293, 314, 435, 693, 1023, 1466, 1785, 1851, 2148
MspR9I	CCNGG	7 cut(s)	253, 693, 775, 1003, 1512, 1542, 1851
MvaI	CCWGG	5 cut(s)	253, 775, 1003, 1512, 1542
MwoI	GCNNNNNNNGC	7 cut(s)	302, 746, 755, 1157, 1373, 1655, 1667
NarI	GGCGCC	1 cut(s)	740
NciI	CCSGG	2 cut(s)	693, 1851
NcoI	CCATGG	1 cut(s)	632
NlaIV	GGNNCC	5 cut(s)	741, 1021, 1375, 1494, 1650
NmuCI	GTSAC	5 cut(s)	656, 858, 1176, 1609, 2050
NsiI	ATGCAT	1 cut(s)	1586
PagI	TCATGA	1 cut(s)	1728
PaqCI	CACCTGC	1 cut(s)	1271
PceI	AGGCCT	1 cut(s)	582
PfeI	GAWTC	5 cut(s)	77, 287, 423, 626, 1296
PfoI	TCCNGGA	1 cut(s)	1540
PkrI	GCNGC	9 cut(s)	592, 676, 958, 1561, 1648, 1660, 1672, 1711, 2163
PleI	GAGTC	9 cut(s)	127, 325, 779, 1051, 1228, 1256, 1553, 1710, 2012
PluTI	GGCGCC	1 cut(s)	743
PpsI	GAGTC	9 cut(s)	127, 325, 779, 1051, 1228, 1256, 1553, 1710, 2012
Ppu21I	YACGTR	1 cut(s)	309
PshAI	GACNNNNGTC	1 cut(s)	1238
PshBI	ATTAAT	1 cut(s)	2277
PsiI	TTATAA	1 cut(s)	1602
Psp6I	CCWGG	5 cut(s)	251, 773, 1001, 1510, 1540
PspEI	GGTNACC	1 cut(s)	680
PspGI	CCWGG	5 cut(s)	251, 773, 1001, 1510, 1540
PspN4I	GGNNCC	5 cut(s)	741, 1021, 1375, 1494, 1650
PspPI	GGNCC	5 cut(s)	826, 1374, 1402, 1493, 1782
PstI	CTGCAG	2 cut(s)	679, 1564
PsuI	RGATCY	3 cut(s)	193, 1309, 1422
PvuII	CAGCTG	1 cut(s)	674
RseI	CAYNNNNRTG	2 cut(s)	98, 1871
SalI	GTCGAC	1 cut(s)	1250
SaqAI	TTAA	6 cut(s)	161, 170, 600, 1419, 2118, 2277
SatI	GCNGC	9 cut(s)	591, 675, 957, 1560, 1647, 1659, 1671, 1710, 2162
Sau96I	GGNCC	5 cut(s)	826, 1374, 1402, 1493, 1782
SchI	GAGTC	9 cut(s)	127, 326, 779, 1051, 1228, 1257, 1554, 1711, 2012
ScrFI	CCNGG	7 cut(s)	253, 693, 775, 1003, 1512, 1542, 1851
SduI	GDGCHC	3 cut(s)	565, 751, 1000
SfaNI	GCATC	2 cut(s)	400, 2188
SfcI	CTRYAG	4 cut(s)	675, 1368, 1560, 2265
SfoI	GGCGCC	1 cut(s)	741
SinI	GGWCC	4 cut(s)	826, 1402, 1493, 1782
SmiMI	CAYNNNNRTG	2 cut(s)	98, 1871
SpeI	ACTAGT	1 cut(s)	1855
Sse9I	AATT	8 cut(s)	278, 597, 834, 939, 1444, 1597, 1753, 2131
SseBI	AGGCCT	1 cut(s)	582
SsiI	CCGC	2 cut(s)	1646, 1659
SspDI	GGCGCC	1 cut(s)	739
StuI	AGGCCT	1 cut(s)	582
StyD4I	CCNGG	7 cut(s)	251, 691, 773, 1001, 1510, 1540, 1849
StyI	CCWWGG	2 cut(s)	632, 1903
TaaI	ACNGT	6 cut(s)	242, 864, 980, 1015, 1078, 2219
TaiI	ACGT	2 cut(s)	311, 2061
TaqI	TCGA	6 cut(s)	565, 624, 930, 1251, 1548, 2190
TasI	AATT	8 cut(s)	278, 597, 834, 939, 1444, 1597, 1753, 2131
TatI	WGTACW	4 cut(s)	216, 762, 980, 1131
TauI	GCSGC	2 cut(s)	1649, 1661
TfiI	GAWTC	5 cut(s)	77, 287, 423, 626, 1296
Tru1I	TTAA	6 cut(s)	161, 170, 600, 1419, 2118, 2277
Tru9I	TTAA	6 cut(s)	161, 170, 600, 1419, 2118, 2277
TscAI	CASTG	7 cut(s)	253, 867, 1051, 1059, 1185, 1234, 1955
TseFI	GTSAC	5 cut(s)	656, 858, 1176, 1609, 2050
TseI	GCWGC	7 cut(s)	590, 674, 956, 1559, 1670, 1709, 2161
Tsp45I	GTSAC	5 cut(s)	656, 858, 1176, 1609, 2050
TspDTI	ATGAA	5 cut(s)	1291, 1635, 1859, 1883, 1940
TspGWI	ACGGA	1 cut(s)	385
TspRI	CASTG	7 cut(s)	253, 867, 1051, 1059, 1185, 1234, 1955
VpaK11BI	GGWCC	4 cut(s)	826, 1402, 1493, 1782
VspI	ATTAAT	1 cut(s)	2277
XapI	RAATTY	1 cut(s)	1444
XbaI	TCTAGA	2 cut(s)	1060, 2092
XmiI	GTMKAC	2 cut(s)	155, 1251
Zsp2I	ATGCAT	1 cut(s)	1586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.