Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002861

Physical Location & Seq

Genomic Coordinates Forward (+)

65049593 .. 65052001

Gene Structure 2409 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G041330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGATCTCTTTGTTACTGTTAATGCTACTGCAAATCAACAATGCAGTGTCAAATGCTGAGGAATACCAGACATATATCATCCACATGGACCATTCCCAAAAACCTGCATCTTTCTTAACACATGAGGCATGGCATCAATCTACCTTAACATCATTAATGCGATCATCTCCTGAAGAACATGATGAGAACGAAATGTTCTTGTACTCATACAACCATGTCATGCACGGCTTCAGTGCAAGGCTCACAGCAACTCAGCTGTCGGAATTGGAGAAATCTCCAGCTCATGTTGCCACACACCCGCAGTTATTTCTTGAGCTGCTCACAACACACAGCCCCAAGTTTCTCGGACTGAAACAGAACTCTGGCATATGGCCAGCTGCGTCTTTTGGCAAAGACGTGATTATAGGTATTATCGACACTGGAATTTGGCCAGAGAGTGAGAGCTTCAATGATAAAGGAATGGCAGAAGTTCCACAGAGATGGAAAGGCACGTGTGAAAATGGAACAGCATTTAGCCCATCTCTCTGCAACAGGAAGCTCATAGGGGCACGTTCTTTTAGCAAAGGGCTCCAAGCTGAAGGCCTAAAAATCCCACCATTTGACTATAATTCACCAAGAGACCACAGTGGTCATGGAACCCACACATCATCAACTGCTGCAGGTAACCATGTAGTCGGTGCAAGTCAATTTGGCTATGCAAAAGGCACGGCAAGAGGTGTGGCGCCGCGTGCACATGTTGCCATGTACAAGGTGCTCTGGGGAGTAGAAACAGAAAAGAGTACAGCCACTGATGTGCTAGCTGCCATGGACCAGGCAATTTCCGACGGAGTTGATATCATATCAATATCTCTTCGCATAAGCCTGCGCGAAGATCCTCCTTATTTCAATAACGTCATTTCCATTGCTTCTCTTTCAGCAGCTGAGAAAGGAATTGTTGTTGTCTGTGCTGCTGGGAATCTGGGTGCTCCGAACTCAATATACAATGGAGCACCCTGGATCACAACAGTAGGAGCTGGCACACTCGATCGTAGTTTCACTGCAACAATGACTCTAGAAAATGGGTTAACAGTTGAAGGAACATCATACTTCCCAGAGAGCATTTACATTACTGATGAACCATTATACTATGGGAAAGAGAATGTGAGCAAAGCAATGTGCTACTATGGGGGATTGGATCATAAAGAAGTTGCTGGAAAGGTAGTCTTATGTGATAATCCCGCCGAGAGCCAGATAGACATTGAAGGACAAAAGGAAGAGGTAGAGAGGGCAGGTGCTTATGCTGGAATCTTCATCACGGATCTCTCATTTTTACATCCATCCGACTACACTGTTCCTGCTATGATTCTGCCATTTGCTACAGGTGCCTTGGTTAGAGATCACGCCACACAGGTCGATACAACCAAAGTTAAGAGCATCACATTTGTTCACACCAACTTGGGAGCAAAACCAGCACCACAAGTGGCAAGTTTCTCTTCAAGAGGACCAGACTCAATCACTCCAGGTATTCTAAAACCAGACATTCTTGCCCCAGGGGTCGATGTGCTGGCTGCAGTGAAACCAGACAAGCCATTCATGGAACTAAAGAATTACAATTTAGTGACAGATTATGCACTAAAATCAGGGACATCAATGGCAGCACCCCATGTCGCTGGCATAGCAGCATTGTTAAAATCTGTTCACCCAGAATGGAGCCCAGCAGCCATCCGATCAGCAATCATGACCACAGCATACGCAGTCGACAACAATCACACCACCATAAAAGACGAATCAGATGGTCTTCCAGCCACACCATTGGACTTTGGGGCAGGCCACATCAACCCAAATAAGGCCATGAACCCCGGACTAGTTTATGACATGAATGTGCAAGACTACATTGAATTTCTATGTACCCTGGGGTACAATGACAAGCAGATGAAAGCTGTTATTAGACGAAGCCAATGGACTTGCAGACAGCCCCCTACTGAATTAAACTACCCTTCTTACATGGTCATCATTAACGGCACAGACTCTCTGAGAACCAAAAGCTTTAGCAGAGTTGTGACAAACGTGGGAGATGACACATCAATTTACCAAGCAATTCTACAAGTTCCTAGTGGGATGAGGATAACAGTAGAGCCTAGTACTCTGACATTCACTAAAAAGTATCAGCAGCAAGGTTTTGTTTTGAACGTAGAGATCGACAGTGATGCTCCGAAGGTGATCTATGGTTATCTCAAATGGGTTGATCAACATGATCACATTGTATCAAGTCCCGTAGTGGCCCTTAATAACAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

758

Amino Acids

82.5

Weight (kDa)

5.71

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	24 - 97	2.4e-16	Peptidase inhibitor I9
Peptidase_S8	PF00082	130 - 584	4.5e-46	Subtilase family
fn3_6	PF17766	655 - 753	6.7e-23	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002861)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34120 FvH4_2g34130 FvH4_2g34150 FvH4_2g34210 FvH4_2g34211 FvH4_2g34212
malus_domestica	MD05G1275700.v1.1 MD05G1275800.v1.1 MD08G1098000.v1.1 MD15G1080200.v1.1 MD15G1080300.v1.1
prunus_persica	Prupe.1G433100_v2.0.a1 Prupe.1G433200_v2.0.a1
pyrus_communis	pycom15g07460
rosa_chinensis	RchiOBHm_Chr6g0308201 RchiOBHm_Chr6g0308211 RchiOBHm_Chr6g0308221
rosa_laevigata	RLG00000010657 RLG00000010658 RLG00000010659
rosa_multiflora	Rmu_sc0005706.1_g000017 Rmu_sc0005706.1_g000018 Rmu_ssc0000355.1_g000001 Rmu_ssc0000355.1_g000006
rosa_roxburghii	Rroxscaffold_7G00160340 Rroxscaffold_7G00160360 Rroxscaffold_7G00160440 Rroxscaffold_7G00160450
rosa_rugosa	Rorug01G0005200 Rorug01G0066300 Rorug06G0364700 Rorug06G0364800 Rorug06G0364900
rosa_samantha	Rh6DG475900 Rh6DG476100 Rh6DG476300
rosa_wichuraiana	Rw6G041330 Rw6G041340 Rw6G041350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1259
Acc36I	ACCTGC	3 cut(s)	112, 650, 1259
AccB1I	GGYRCC	2 cut(s)	721, 1361
AccI	GTMKAC	1 cut(s)	1737
AccII	CGCG	2 cut(s)	727, 867
AciI	CCGC	3 cut(s)	299, 725, 1218
AclWI	GGATC	4 cut(s)	866, 1004, 1182, 1305
AcoI	YGGCCR	2 cut(s)	371, 428
AcsI	RAATTY	2 cut(s)	423, 1877
AcuI	CTGAAG	3 cut(s)	192, 214, 597
AcvI	CACGTG	1 cut(s)	492
AcyI	GRCGYC	1 cut(s)	722
AfaI	GTAC	6 cut(s)	203, 746, 781, 1888, 1898, 2122
AfiI	CCNNNNNNNGG	1 cut(s)	1825
AflIII	ACRYGT	2 cut(s)	491, 733
AgsI	TTSAA	7 cut(s)	448, 886, 1073, 1241, 1476, 1877, 2167
AhlI	ACTAGT	1 cut(s)	1843
AjiI	CACGTC	1 cut(s)	397
AjnI	CCWGG	5 cut(s)	810, 992, 1498, 1528, 1890
AleI	CACNNNNGTG	1 cut(s)	791
Alw21I	GWGCWC	4 cut(s)	733, 756, 967, 991
Alw26I	GTCTC	1 cut(s)	612
Alw44I	GTGCAC	1 cut(s)	729
AlwI	GGATC	4 cut(s)	866, 1004, 1182, 1305
AlwNI	CAGNNNCTG	2 cut(s)	788, 920
AoxI	GGCC	6 cut(s)	371, 428, 580, 1807, 1827, 2259
ApaLI	GTGCAC	1 cut(s)	729
ApoI	RAATTY	2 cut(s)	423, 1877
AseI	ATTAAT	1 cut(s)	155
AspLEI	GCGC	2 cut(s)	724, 867
AspS9I	GGNCC	4 cut(s)	88, 808, 1481, 2260
AsuC2I	CCSGG	1 cut(s)	1839
AsuHPI	GGTGA	3 cut(s)	603, 1670, 2209
AsuNHI	GCTAGC	1 cut(s)	796
AvaII	GGWCC	3 cut(s)	88, 808, 1481
BaeGI	GKGCMC	2 cut(s)	550, 733
BalI	TGGCCA	2 cut(s)	373, 430
BanI	GGYRCC	2 cut(s)	721, 1361
BanII	GRGCYC	2 cut(s)	570, 1694
BbrPI	CACGTG	1 cut(s)	492
BbsI	GAAGAC	1 cut(s)	1769
Bbv12I	GWGCWC	4 cut(s)	733, 756, 967, 991
BbvCI	CCTCAGC	1 cut(s)	57
BccI	CCATC	5 cut(s)	474, 526, 1324, 1709, 1766
BceAI	ACGGC	3 cut(s)	241, 723, 2014
BcgI	CGANNNNNNTGC	2 cut(s)	2168, 2202
BciT130I	CCWGG	5 cut(s)	812, 994, 1500, 1530, 1892
BclI	TGATCA	2 cut(s)	2224, 2233
BcnI	CCSGG	1 cut(s)	1839
BcoDI	GTCTC	1 cut(s)	612
BcuI	ACTAGT	1 cut(s)	1843
BfaI	CTAG	6 cut(s)	797, 1052, 1844, 2091, 2118, 2275
BfmI	CTRYAG	3 cut(s)	657, 1356, 1548
BfoI	RGCGCY	1 cut(s)	725
BfuAI	ACCTGC	3 cut(s)	112, 650, 1259
BmcAI	AGTACT	1 cut(s)	2122
Bme1390I	CCNGG	6 cut(s)	812, 994, 1500, 1530, 1839, 1892
Bme18I	GGWCC	3 cut(s)	88, 808, 1481
BmgBI	CACGTC	1 cut(s)	397
BmgT120I	GGNCC	4 cut(s)	88, 808, 1481, 2260
BmiI	GGNNCC	5 cut(s)	569, 637, 723, 1363, 1691
BmrFI	CCNGG	6 cut(s)	812, 994, 1500, 1530, 1839, 1892
BmsI	GCATC	4 cut(s)	116, 142, 1422, 2176
BmtI	GCTAGC	1 cut(s)	800
BpiI	GAAGAC	1 cut(s)	1769
BpmI	CTGGAG	2 cut(s)	261, 1482
Bpu10I	CCTNAGC	1 cut(s)	57
BpuEI	CTTGAG	1 cut(s)	332
BpuMI	CCSGG	1 cut(s)	1839
BsaAI	YACGTR	1 cut(s)	492
BsaHI	GRCGYC	1 cut(s)	722
BsaI	GGTCTC	1 cut(s)	612
BsaJI	CCNNGG	8 cut(s)	804, 992, 1365, 1528, 1529, 1837, 1890, 1891
Bsc4I	CCNNNNNNNGG	1 cut(s)	1825
Bse1I	ACTGG	1 cut(s)	424
Bse3DI	GCAATG	2 cut(s)	900, 1158
BseBI	CCWGG	5 cut(s)	812, 994, 1500, 1530, 1892
BseDI	CCNNGG	8 cut(s)	804, 992, 1365, 1528, 1529, 1837, 1890, 1891
BseGI	GGATG	5 cut(s)	78, 1312, 1316, 1701, 2103
BseLI	CCNNNNNNNGG	1 cut(s)	1825
BseMI	GCAATG	2 cut(s)	900, 1158
BseMII	CTCAG	4 cut(s)	48, 266, 912, 2003
BseNI	ACTGG	1 cut(s)	424
BseSI	GKGCMC	2 cut(s)	550, 733
BseYI	CCCAGC	2 cut(s)	950, 1693
Bsh1236I	CGCG	2 cut(s)	727, 867
Bsh1285I	CGRYCG	1 cut(s)	1027
BshFI	GGCC	6 cut(s)	373, 430, 582, 1809, 1829, 2261
BshNI	GGYRCC	2 cut(s)	721, 1361
BsiEI	CGRYCG	1 cut(s)	1027
BsiHKAI	GWGCWC	4 cut(s)	733, 756, 967, 991
BsiSI	CCGG	1 cut(s)	1839
BslFI	GGGAC	2 cut(s)	1636, 2235
BslI	CCNNNNNNNGG	1 cut(s)	1825
BsmAI	GTCTC	1 cut(s)	612
BsmFI	GGGAC	2 cut(s)	1636, 2235
BsnI	GGCC	6 cut(s)	373, 430, 582, 1809, 1829, 2261
Bso31I	GGTCTC	1 cut(s)	612
Bsp1286I	GDGCHC	7 cut(s)	550, 570, 733, 756, 967, 991, 1694
Bsp1407I	TGTACA	1 cut(s)	744
Bsp19I	CCATGG	1 cut(s)	804
BspACI	CCGC	3 cut(s)	299, 725, 1218
BspANI	GGCC	6 cut(s)	373, 430, 582, 1809, 1829, 2261
BspCNI	CTCAG	4 cut(s)	49, 265, 913, 2004
BspFNI	CGCG	2 cut(s)	727, 867
BspHI	TCATGA	1 cut(s)	1716
BspLI	GGNNCC	5 cut(s)	569, 637, 723, 1363, 1691
BspMAI	CTGCAG	2 cut(s)	661, 1552
BspMI	ACCTGC	3 cut(s)	112, 650, 1259
BspOI	GCTAGC	1 cut(s)	800
BspPI	GGATC	4 cut(s)	866, 1004, 1182, 1305
BspT107I	GGYRCC	2 cut(s)	721, 1361
BspTNI	GGTCTC	1 cut(s)	612
BsrDI	GCAATG	2 cut(s)	900, 1158
BsrGI	TGTACA	1 cut(s)	744
BsrI	ACTGG	1 cut(s)	424
BssECI	CCNNGG	8 cut(s)	804, 992, 1365, 1528, 1529, 1837, 1890, 1891
BssNI	GRCGYC	1 cut(s)	722
BssT1I	CCWWGG	2 cut(s)	804, 1365
Bst2UI	CCWGG	5 cut(s)	812, 994, 1500, 1530, 1892
Bst4CI	ACNGT	7 cut(s)	18, 626, 1006, 1069, 1330, 2110, 2183
Bst6I	CTCTTC	3 cut(s)	855, 1248, 1477
BstACI	GRCGYC	1 cut(s)	722
BstAPI	GCANNNNNTGC	1 cut(s)	737
BstAUI	TGTACA	1 cut(s)	744
BstBAI	YACGTR	1 cut(s)	492
BstC8I	GCNNGC	8 cut(s)	375, 729, 798, 863, 1015, 1545, 1651, 1807
BstDEI	CTNAG	4 cut(s)	57, 252, 921, 2012
BstDSI	CCRYGG	1 cut(s)	804
BstEII	GGTNACC	1 cut(s)	662
BstF5I	GGATG	5 cut(s)	78, 1312, 1316, 1701, 2103
BstFNI	CGCG	2 cut(s)	727, 867
BstH2I	RGCGCY	1 cut(s)	725
BstHHI	GCGC	2 cut(s)	724, 867
BstMAI	GTCTC	1 cut(s)	612
BstMCI	CGRYCG	1 cut(s)	1027
BstMWI	GCNNNNNNNGC	6 cut(s)	287, 728, 737, 1361, 1448, 1655
BstNI	CCWGG	5 cut(s)	812, 994, 1500, 1530, 1892
BstNSI	RCATGY	1 cut(s)	737
BstPI	GGTNACC	1 cut(s)	662
BstSCI	CCNGG	6 cut(s)	810, 992, 1498, 1528, 1837, 1890
BstSFI	CTRYAG	3 cut(s)	657, 1356, 1548
BstSLI	GKGCMC	2 cut(s)	550, 733
BstUI	CGCG	2 cut(s)	727, 867
BstV2I	GAAGAC	1 cut(s)	1769
BstX2I	RGATCY	2 cut(s)	871, 1297
BstXI	CCANNNNNNTGG	3 cut(s)	480, 1649, 1792
BstYI	RGATCY	2 cut(s)	871, 1297
BsuRI	GGCC	6 cut(s)	373, 430, 582, 1809, 1829, 2261
BtgI	CCRYGG	1 cut(s)	804
BtrI	CACGTC	1 cut(s)	397
BtsCI	GGATG	5 cut(s)	78, 1312, 1316, 1701, 2103
BtsI	GCAGTG	3 cut(s)	51, 1035, 1557
BtsIMutI	CAGTG	9 cut(s)	51, 238, 417, 631, 786, 1035, 1326, 1557, 2188
BveI	ACCTGC	3 cut(s)	112, 650, 1259
Cac8I	GCNNGC	8 cut(s)	375, 729, 798, 863, 1015, 1545, 1651, 1807
CaiI	CAGNNNCTG	2 cut(s)	788, 920
CciI	TCATGA	1 cut(s)	1716
CfoI	GCGC	2 cut(s)	724, 867
Cfr13I	GGNCC	4 cut(s)	88, 808, 1481, 2260
CseI	GACGC	1 cut(s)	369
Csp6I	GTAC	6 cut(s)	202, 745, 780, 1887, 1897, 2121
CspCI	CAANNNNNGTGG	2 cut(s)	699, 734
CviQI	GTAC	6 cut(s)	202, 745, 780, 1887, 1897, 2121
DdeI	CTNAG	4 cut(s)	57, 252, 921, 2012
DinI	GGCGCC	1 cut(s)	723
EaeI	YGGCCR	2 cut(s)	371, 428
Eam1104I	CTCTTC	3 cut(s)	855, 1248, 1477
EarI	CTCTTC	3 cut(s)	855, 1248, 1477
Eco130I	CCWWGG	2 cut(s)	804, 1365
Eco147I	AGGCCT	1 cut(s)	582
Eco24I	GRGCYC	2 cut(s)	570, 1694
Eco31I	GGTCTC	1 cut(s)	612
Eco32I	GATATC	1 cut(s)	835
Eco47I	GGWCC	3 cut(s)	88, 808, 1481
Eco57I	CTGAAG	3 cut(s)	192, 214, 597
Eco72I	CACGTG	1 cut(s)	492
Eco91I	GGTNACC	1 cut(s)	662
EcoO65I	GGTNACC	1 cut(s)	662
EcoRII	CCWGG	5 cut(s)	810, 992, 1498, 1528, 1890
EcoRV	GATATC	1 cut(s)	835
EcoT14I	CCWWGG	2 cut(s)	804, 1365
EcoT38I	GRGCYC	2 cut(s)	570, 1694
EgeI	GGCGCC	1 cut(s)	723
EheI	GGCGCC	1 cut(s)	723
ErhI	CCWWGG	2 cut(s)	804, 1365
FalI	AAGNNNNNCTT	4 cut(s)	1187, 1219, 1456, 1488
FaqI	GGGAC	2 cut(s)	1636, 2235
FauI	CCCGC	2 cut(s)	306, 1225
FauNDI	CATATG	1 cut(s)	368
FbaI	TGATCA	2 cut(s)	2224, 2233
FblI	GTMKAC	1 cut(s)	1737
FokI	GGATG	5 cut(s)	65, 1299, 1303, 1688, 2110
FriOI	GRGCYC	2 cut(s)	570, 1694
FspBI	CTAG	6 cut(s)	797, 1052, 1844, 2091, 2118, 2275
GlaI	GCGC	2 cut(s)	723, 866
GsaI	CCCAGC	2 cut(s)	954, 1697
GsuI	CTGGAG	2 cut(s)	261, 1482
HaeII	RGCGCY	1 cut(s)	725
HaeIII	GGCC	6 cut(s)	373, 430, 582, 1809, 1829, 2261
HapII	CCGG	1 cut(s)	1839
HgaI	GACGC	1 cut(s)	369
HhaI	GCGC	2 cut(s)	724, 867
Hin1I	GRCGYC	1 cut(s)	722
Hin6I	GCGC	2 cut(s)	722, 865
HinP1I	GCGC	2 cut(s)	722, 865
HincII	GTYRAC	2 cut(s)	1065, 1738
HindII	GTYRAC	2 cut(s)	1065, 1738
HindIII	AAGCTT	1 cut(s)	2023
HinfI	GANTC	7 cut(s)	955, 1048, 1284, 1342, 1487, 1766, 2006
HpaI	GTTAAC	1 cut(s)	1065
HpaII	CCGG	1 cut(s)	1839
HphI	GGTGA	3 cut(s)	603, 1670, 2209
Hpy166II	GTNNAC	5 cut(s)	731, 1065, 1426, 1678, 1738
Hpy188III	TCNNGA	5 cut(s)	170, 311, 1052, 1476, 1717
Hpy8I	GTNNAC	5 cut(s)	731, 1065, 1426, 1678, 1738
Hpy99I	CGWCG	1 cut(s)	827
HpyAV	CCTTC	5 cut(s)	572, 1067, 1235, 1986, 2188
HpyCH4III	ACNGT	7 cut(s)	18, 626, 1006, 1069, 1330, 2110, 2183
HpyCH4IV	ACGT	6 cut(s)	396, 491, 550, 891, 2046, 2169
HpyF10VI	GCNNNNNNNGC	6 cut(s)	287, 728, 737, 1361, 1448, 1655
HpyF3I	CTNAG	4 cut(s)	57, 252, 921, 2012
HpySE526I	ACGT	6 cut(s)	396, 491, 550, 891, 2046, 2169
Hsp92I	GRCGYC	1 cut(s)	722
HspAI	GCGC	2 cut(s)	722, 865
KasI	GGCGCC	1 cut(s)	721
Ksp22I	TGATCA	2 cut(s)	2224, 2233
KspAI	GTTAAC	1 cut(s)	1065
LmnI	GCTCC	7 cut(s)	573, 970, 986, 1010, 1439, 1689, 2194
LweI	GCATC	4 cut(s)	116, 142, 1422, 2176
MaeI	CTAG	6 cut(s)	797, 1052, 1844, 2091, 2118, 2275
MaeII	ACGT	6 cut(s)	396, 491, 550, 891, 2046, 2169
MaeIII	GTNAC	4 cut(s)	12, 662, 1597, 2038
MboII	GAAGA	7 cut(s)	185, 842, 881, 1265, 1279, 1464, 1769
MflI	RGATCY	2 cut(s)	871, 1297
MhlI	GDGCHC	7 cut(s)	550, 570, 733, 756, 967, 991, 1694
MlsI	TGGCCA	2 cut(s)	373, 430
MluNI	TGGCCA	2 cut(s)	373, 430
Mly113I	GGCGCC	1 cut(s)	722
MlyI	GAGTC	3 cut(s)	1042, 1481, 2000
MmeI	TCCRAC	3 cut(s)	240, 846, 1344
MnlI	CCTC	8 cut(s)	52, 118, 707, 885, 1249, 1257, 1472, 2094
Mox20I	TGGCCA	2 cut(s)	373, 430
MscI	TGGCCA	2 cut(s)	373, 430
MslI	CAYNNNNRTG	4 cut(s)	83, 478, 791, 1859
Msp20I	TGGCCA	2 cut(s)	373, 430
MspA1I	CMGCKG	3 cut(s)	256, 377, 920
MspI	CCGG	1 cut(s)	1839
MspR9I	CCNGG	6 cut(s)	812, 994, 1500, 1530, 1839, 1892
MvaI	CCWGG	5 cut(s)	812, 994, 1500, 1530, 1892
MvnI	CGCG	2 cut(s)	727, 867
MwoI	GCNNNNNNNGC	6 cut(s)	287, 728, 737, 1361, 1448, 1655
NarI	GGCGCC	1 cut(s)	722
NciI	CCSGG	1 cut(s)	1839
NcoI	CCATGG	1 cut(s)	804
NdeI	CATATG	1 cut(s)	368
NheI	GCTAGC	1 cut(s)	796
NlaIV	GGNNCC	5 cut(s)	569, 637, 723, 1363, 1691
NmeAIII	GCCGAG	1 cut(s)	1246
NmuCI	GTSAC	2 cut(s)	1597, 2038
NspI	RCATGY	1 cut(s)	737
OliI	CACNNNNGTG	1 cut(s)	791
PagI	TCATGA	1 cut(s)	1716
PaqCI	CACCTGC	1 cut(s)	1259
PasI	CCCWGGG	2 cut(s)	1529, 1891
PceI	AGGCCT	1 cut(s)	582
PciI	ACATGT	1 cut(s)	733
PcsI	WCGNNNNNNNCGW	1 cut(s)	2175
PfeI	GAWTC	4 cut(s)	955, 1284, 1342, 1766
Ple19I	CGATCG	1 cut(s)	1027
PleI	GAGTC	3 cut(s)	1042, 1481, 2000
PluTI	GGCGCC	1 cut(s)	725
PmaCI	CACGTG	1 cut(s)	492
PmlI	CACGTG	1 cut(s)	492
PpsI	GAGTC	3 cut(s)	1042, 1481, 2000
Ppu21I	YACGTR	1 cut(s)	492
PscI	ACATGT	1 cut(s)	733
PshBI	ATTAAT	1 cut(s)	155
Psp6I	CCWGG	5 cut(s)	810, 992, 1498, 1528, 1890
PspCI	CACGTG	1 cut(s)	492
PspEI	GGTNACC	1 cut(s)	662
PspFI	CCCAGC	2 cut(s)	950, 1693
PspGI	CCWGG	5 cut(s)	810, 992, 1498, 1528, 1890
PspN4I	GGNNCC	5 cut(s)	569, 637, 723, 1363, 1691
PspPI	GGNCC	4 cut(s)	88, 808, 1481, 2260
PsrI	GAACNNNNNNTAC	2 cut(s)	962, 994
PstI	CTGCAG	2 cut(s)	661, 1552
PstNI	CAGNNNCTG	2 cut(s)	788, 920
PsuI	RGATCY	2 cut(s)	871, 1297
PvuI	CGATCG	1 cut(s)	1027
PvuII	CAGCTG	3 cut(s)	256, 377, 920
RsaI	GTAC	6 cut(s)	203, 746, 781, 1888, 1898, 2122
RsaNI	GTAC	6 cut(s)	202, 745, 780, 1887, 1897, 2121
RseI	CAYNNNNRTG	4 cut(s)	83, 478, 791, 1859
SalI	GTCGAC	1 cut(s)	1736
Sau96I	GGNCC	4 cut(s)	88, 808, 1481, 2260
ScaI	AGTACT	1 cut(s)	2122
SchI	GAGTC	3 cut(s)	1042, 1481, 2000
ScrFI	CCNGG	6 cut(s)	812, 994, 1500, 1530, 1839, 1892
SduI	GDGCHC	7 cut(s)	550, 570, 733, 756, 967, 991, 1694
SfaNI	GCATC	4 cut(s)	116, 142, 1422, 2176
SfcI	CTRYAG	3 cut(s)	657, 1356, 1548
SfoI	GGCGCC	1 cut(s)	723
SinI	GGWCC	3 cut(s)	88, 808, 1481
SmiMI	CAYNNNNRTG	4 cut(s)	83, 478, 791, 1859
SmlI	CTYRAG	1 cut(s)	311
SmoI	CTYRAG	1 cut(s)	311
SpeI	ACTAGT	1 cut(s)	1843
SseBI	AGGCCT	1 cut(s)	582
SsiI	CCGC	3 cut(s)	299, 725, 1218
SspDI	GGCGCC	1 cut(s)	721
SspMI	CTAG	6 cut(s)	797, 1052, 1844, 2091, 2118, 2275
StuI	AGGCCT	1 cut(s)	582
StyD4I	CCNGG	6 cut(s)	810, 992, 1498, 1528, 1837, 1890
StyI	CCWWGG	2 cut(s)	804, 1365
TaaI	ACNGT	7 cut(s)	18, 626, 1006, 1069, 1330, 2110, 2183
TaiI	ACGT	6 cut(s)	399, 494, 553, 894, 2049, 2172
TaqI	TCGA	6 cut(s)	414, 1023, 1392, 1536, 1737, 2178
TatI	WGTACW	4 cut(s)	201, 744, 779, 2120
TauI	GCSGC	1 cut(s)	727
TfiI	GAWTC	4 cut(s)	955, 1284, 1342, 1766
TscAI	CASTG	9 cut(s)	51, 238, 424, 631, 793, 1042, 1333, 1557, 2188
TseFI	GTSAC	2 cut(s)	1597, 2038
Tsp45I	GTSAC	2 cut(s)	1597, 2038
TspDTI	ATGAA	6 cut(s)	1128, 1279, 1561, 1847, 1871, 1928
TspGWI	ACGGA	2 cut(s)	840, 1310
TspRI	CASTG	9 cut(s)	51, 238, 424, 631, 793, 1042, 1333, 1557, 2188
VneI	GTGCAC	1 cut(s)	729
VpaK11BI	GGWCC	3 cut(s)	88, 808, 1481
VspI	ATTAAT	1 cut(s)	155
XapI	RAATTY	2 cut(s)	423, 1877
XbaI	TCTAGA	1 cut(s)	1051
XceI	RCATGY	1 cut(s)	737
XcmI	CCANNNNNNNNNTGG	1 cut(s)	629
XmiI	GTMKAC	1 cut(s)	1737
XspI	CTAG	6 cut(s)	797, 1052, 1844, 2091, 2118, 2275
ZrmI	AGTACT	1 cut(s)	2122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.