Rorug06G0364700

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002861
Physical Location & Seq
Forward (+)
51223405 .. 51225297
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0364700.1

Sequence Viewer

Length: 1539 bp
ATGACAGAGTTACTCATGCTAAGCTCAGCTATGGAGCTCGAGCTCGAGCTCGACCTCTCCGGCTGCAGCACTGAAACCACAAGCACGACCAACACGGCCATTTCCCCTGACCAGTGGTACGATTGGTCTCCGGTCGTCGACTGGGAGTCTCTCGCTCCGATTGACCAGCTCGCCGGTGGTGGAGACTTCCACGAGCTGTTCGAGTCGATGATGGAGGGCCAAGACGGCGGCGATATGGACTCGCCGTCCTCCGAGACCGTTGAGGAAGTGTCAAACTCGTGTTACACCACGACGTCGTCGCAGTCGACGGACGTGACGATGCTGGAGGAAGGCGCGGCATCGAACGGCGAGGATTCCAAGGGGCTGAGATTGGTGCATCTGTTGATGGCGGCGGCGGAGGCGCTGACCGGCGCCAACAAGAGCAGAGATCTGGCTCGGGTGATATTGGTTCGGCTCAAGGAGTTGGTCTCCCCAACTGACGGTACTAACATGGAGAGGCTGGCGGCGTATTTTACCGAGGCGCTTCAGGGTTTGCTAGAAGGCGCCGGGGGTGTCAGCCACTCCAAGCATTTGTTCGGAAATGGGCCCCACCGCGACCACGGCCACCACCCGACGGACGTCATCGCGGCGTTTCAGCTACTTCAGGACATGTCGCCCTATGTCAAGTTTGGGCACTTCACGGCCAATCAGGCCATTTTGGAGACTGTGGCCCATGACAGGAGGGTCCACATAGTGGACTATGACATCATGGAGGGAATCCAATGGGCCTCCTTGATGCAGGCCCTGGTGTCCAGGAAGGATGGCCCACCGACCCCACATCTTAGGATCACTGCTTTGTCGCGAGGCGGGAGCAGCCGGAGGTCGATAGGGACCGTCCAAGAGACCGGTCGTCGTCTCACGGCGTTTGCCACGTCCATTGGTCAGCCGTTTTCGTTTCATCAATGTAGGTTGGATTCGGACGAGACATTTCGACCGTCGGCTCTAAAGTTGGTTAAGGGCGAGGCCTTGGTAATAAACTGCATGCTGAACCTTCCACACTTTAGCTATCGATCCCCGGATTCTATTGCTTCCTTTTTATCCGGAGCCAAGACCCTAAACCCGAGACTGGTAACTTTGGTTGAAGAAGAGGTTAGGCCTGCAGGAGACGGTGGCTTTGTGGCCCGCTTCATGGACTCACTGTATCACTACTCGGCTGTCTACGACTCGCTCGAGGCCGGCTTCCCAATGCAGAGCCGGGCTCGAGAATTAGTGGAGAGAGTGTTCTTGGGACCCCGAATAGCTGGGTCGTTGGCTCGTATCTATCGGGCTCACGGAGAGGTAGGTTGCTCTTGGTGGGAGTGGTTAGGTGCGGTAGGGTTTAAGCCAATGCCAATAAGCTTTGCCAATCATTGTCAGGCAAAGCTTTTGTTGGGTCTTTTTAATGATGGTTATAGGGTGGAGGAGGTGGCAAATCATAGGTTGGTTTTAGGTTGGAAATCACGGTCTTTACTATCAGCTTCTATTTGGACTTCACCCCCAGAATCTGATTTTGCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

512

Amino Acids

56.15

Weight (kDa)

5.2

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 124 - 503 4.4e-104 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 722
AatII GACGTC 2 cut(s) 296, 621
AccB1I GGYRCC 2 cut(s) 410, 542
AccB7I CCANNNNNTGG 1 cut(s) 733
AccI GTMKAC 3 cut(s) 138, 305, 1197
AccII CGCG 4 cut(s) 335, 594, 626, 841
AccIII TCCGGA 1 cut(s) 1079
AclWI GGATC 2 cut(s) 833, 1044
AcoI YGGCCR 3 cut(s) 96, 601, 681
AcuI CTGAAG 2 cut(s) 509, 626
AcyI GRCGYC 4 cut(s) 293, 411, 543, 618
AdeI CACNNNGTG 1 cut(s) 733
AfaI GTAC 2 cut(s) 119, 484
AfiI CCNNNNNNNGG 6 cut(s) 479, 613, 717, 733, 1105, 1168
AflIII ACRYGT 1 cut(s) 648
AgeI ACCGGT 1 cut(s) 884
AgsI TTSAA 1 cut(s) 1121
AhdI GACNNNNNGTC 3 cut(s) 145, 244, 888
AjiI CACGTC 2 cut(s) 313, 912
AjnI CCWGG 2 cut(s) 783, 791
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AloI GAACNNNNNNTCC 2 cut(s) 1244, 1276
Alw21I GWGCWC 3 cut(s) 39, 45, 51
AlwI GGATC 2 cut(s) 833, 1044
AlwNI CAGNNNCTG 2 cut(s) 784, 1523
Ama87I CYCGRG 6 cut(s) 38, 44, 435, 1099, 1208, 1239
Aor13HI TCCGGA 1 cut(s) 1079
ApaI GGGCCC 1 cut(s) 588
ApeKI GCWGC 3 cut(s) 63, 66, 852
ArsI GACNNNNNNTTYG 2 cut(s) 1136, 1168
AsiGI ACCGGT 1 cut(s) 884
AspLEI GCGC 5 cut(s) 335, 403, 413, 523, 545
AsuC2I CCSGG 3 cut(s) 547, 1055, 1235
AsuHPI GGTGA 2 cut(s) 451, 1503
AvaI CYCGRG 6 cut(s) 38, 44, 435, 1099, 1208, 1239
AvaII GGWCC 3 cut(s) 724, 870, 1268
BaeGI GKGCMC 2 cut(s) 588, 675
BanI GGYRCC 2 cut(s) 410, 542
BanII GRGCYC 6 cut(s) 39, 45, 51, 588, 1240, 1309
BauI CACGAG 2 cut(s) 191, 277
Bbv12I GWGCWC 3 cut(s) 39, 45, 51
BbvI GCAGC 3 cut(s) 50, 78, 864
BccI CCATC 4 cut(s) 205, 379, 794, 1418
BceAI ACGGC 8 cut(s) 111, 229, 241, 361, 616, 696, 910, 915
BciT130I CCWGG 2 cut(s) 785, 793
BcnI CCSGG 3 cut(s) 547, 1055, 1235
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 2 cut(s) 64, 1137
BfoI RGCGCY 4 cut(s) 404, 414, 524, 546
BglI GCCNNNNNGGC 2 cut(s) 225, 689
BglII AGATCT 1 cut(s) 427
BisI GCNGC 9 cut(s) 64, 67, 229, 336, 390, 393, 504, 627, 853
BlpI GCTNAGC 2 cut(s) 20, 25
BlsI GCNGC 9 cut(s) 65, 68, 230, 337, 391, 394, 505, 628, 854
Bme1390I CCNGG 5 cut(s) 547, 785, 793, 1055, 1235
Bme18I GGWCC 3 cut(s) 724, 870, 1268
BmeRI GACNNNNNGTC 3 cut(s) 145, 244, 888
BmeT110I CYCGRG 6 cut(s) 38, 44, 435, 1099, 1208, 1239
BmgBI CACGTC 2 cut(s) 313, 912
BmiI GGNNCC 9 cut(s) 412, 544, 586, 587, 725, 871, 1084, 1269, 1270
BmrFI CCNGG 5 cut(s) 547, 785, 793, 1055, 1235
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 4 cut(s) 309, 347, 385, 765
BmuI ACTGGG 1 cut(s) 151
BoxI GACNNNNGTC 1 cut(s) 617
BplI GAGNNNNNCTC 4 cut(s) 452, 484, 1222, 1254
BpmI CTGGAG 1 cut(s) 344
Bpu1102I GCTNAGC 2 cut(s) 20, 25
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 3 cut(s) 547, 1055, 1235
Bsa29I ATCGAT 1 cut(s) 1048
BsaHI GRCGYC 4 cut(s) 293, 411, 543, 618
BsaI GGTCTC 4 cut(s) 132, 248, 472, 875
BsaJI CCNNGG 7 cut(s) 357, 516, 546, 598, 783, 1005, 1053
BsaWI WCCGGW 3 cut(s) 130, 884, 1079
BsaXI ACNNNNNCTCC 2 cut(s) 1244, 1274
Bsc4I CCNNNNNNNGG 6 cut(s) 479, 613, 717, 733, 1105, 1168
Bse118I RCCGGY 4 cut(s) 173, 407, 884, 1214
Bse1I ACTGG 3 cut(s) 112, 146, 1110
BseAI TCCGGA 1 cut(s) 1079
BseBI CCWGG 2 cut(s) 785, 793
BseCI ATCGAT 1 cut(s) 1048
BseDI CCNNGG 7 cut(s) 357, 516, 546, 598, 783, 1005, 1053
BseGI GGATG 1 cut(s) 805
BseLI CCNNNNNNNGG 6 cut(s) 479, 613, 717, 733, 1105, 1168
BseMII CTCAG 2 cut(s) 39, 356
BseNI ACTGG 3 cut(s) 112, 146, 1110
BseRI GAGGAG 1 cut(s) 1454
BseSI GKGCMC 2 cut(s) 588, 675
BseXI GCAGC 3 cut(s) 50, 78, 864
BseYI CCCAGC 1 cut(s) 1280
Bsh1236I CGCG 4 cut(s) 335, 594, 626, 841
Bsh1285I CGRYCG 3 cut(s) 135, 889, 974
BshNI GGYRCC 2 cut(s) 410, 542
BshTI ACCGGT 1 cut(s) 884
BshVI ATCGAT 1 cut(s) 1048
BsiEI CGRYCG 3 cut(s) 135, 889, 974
BsiHKAI GWGCWC 3 cut(s) 39, 45, 51
BsiHKCI CYCGRG 6 cut(s) 38, 44, 435, 1099, 1208, 1239
BslFI GGGAC 2 cut(s) 883, 1281
BslI CCNNNNNNNGG 6 cut(s) 479, 613, 717, 733, 1105, 1168
BsmBI CGTCTC 2 cut(s) 899, 1137
BsmFI GGGAC 2 cut(s) 883, 1281
Bso31I GGTCTC 4 cut(s) 132, 248, 472, 875
BsoBI CYCGRG 6 cut(s) 38, 44, 435, 1099, 1208, 1239
Bsp120I GGGCCC 1 cut(s) 584
Bsp1286I GDGCHC 7 cut(s) 39, 45, 51, 588, 675, 1240, 1309
Bsp13I TCCGGA 1 cut(s) 1079
Bsp143I GATC 3 cut(s) 427, 825, 1049
Bsp1720I GCTNAGC 2 cut(s) 20, 25
Bsp68I TCGCGA 1 cut(s) 841
BspCNI CTCAG 2 cut(s) 38, 357
BspDI ATCGAT 1 cut(s) 1048
BspEI TCCGGA 1 cut(s) 1079
BspFNI CGCG 4 cut(s) 335, 594, 626, 841
BspLI GGNNCC 9 cut(s) 412, 544, 586, 587, 725, 871, 1084, 1269, 1270
BspMAI CTGCAG 2 cut(s) 68, 1141
BspPI GGATC 2 cut(s) 833, 1044
BspT107I GGYRCC 2 cut(s) 410, 542
BspTNI GGTCTC 4 cut(s) 132, 248, 472, 875
BsrFI RCCGGY 4 cut(s) 173, 407, 884, 1214
BsrI ACTGG 3 cut(s) 112, 146, 1110
BssAI RCCGGY 4 cut(s) 173, 407, 884, 1214
BssECI CCNNGG 7 cut(s) 357, 516, 546, 598, 783, 1005, 1053
BssMI GATC 3 cut(s) 427, 825, 1049
BssNI GRCGYC 4 cut(s) 293, 411, 543, 618
BssSI CACGAG 2 cut(s) 191, 277
BssT1I CCWWGG 2 cut(s) 357, 1005
Bst2BI CACGAG 2 cut(s) 191, 277
Bst2UI CCWGG 2 cut(s) 785, 793
Bst4CI ACNGT 8 cut(s) 259, 482, 706, 874, 975, 1148, 1179, 1482
Bst6I CTCTTC 1 cut(s) 1119
BstACI GRCGYC 4 cut(s) 293, 411, 543, 618
BstC8I GCNNGC 7 cut(s) 171, 501, 780, 1022, 1137, 1162, 1216
BstDEI CTNAG 4 cut(s) 20, 25, 365, 821
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 1 cut(s) 805
BstFNI CGCG 4 cut(s) 335, 594, 626, 841
BstH2I RGCGCY 4 cut(s) 404, 414, 524, 546
BstHHI GCGC 5 cut(s) 335, 403, 413, 523, 545
BstKTI GATC 3 cut(s) 430, 828, 1052
BstMBI GATC 3 cut(s) 427, 825, 1049
BstMCI CGRYCG 3 cut(s) 135, 889, 974
BstMWI GCNNNNNNNGC 5 cut(s) 225, 398, 600, 689, 852
BstNI CCWGG 2 cut(s) 785, 793
BstNSI RCATGY 2 cut(s) 652, 1024
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 5 cut(s) 545, 783, 791, 1053, 1233
BstSFI CTRYAG 2 cut(s) 64, 1137
BstSLI GKGCMC 2 cut(s) 588, 675
BstUI CGCG 4 cut(s) 335, 594, 626, 841
BstV1I GCAGC 3 cut(s) 50, 78, 864
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 1048
BsuTUI ATCGAT 1 cut(s) 1048
BtgI CCRYGG 1 cut(s) 598
BtgZI GCGATG 1 cut(s) 607
BtrI CACGTC 2 cut(s) 313, 912
BtsCI GGATG 1 cut(s) 805
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 4 cut(s) 69, 119, 828, 1175
BtuMI TCGCGA 1 cut(s) 841
Cac8I GCNNGC 7 cut(s) 171, 501, 780, 1022, 1137, 1162, 1216
CaiI CAGNNNCTG 2 cut(s) 784, 1523
CfoI GCGC 5 cut(s) 335, 403, 413, 523, 545
Cfr10I RCCGGY 4 cut(s) 173, 407, 884, 1214
ClaI ATCGAT 1 cut(s) 1048
Csp6I GTAC 2 cut(s) 118, 483
CspAI ACCGGT 1 cut(s) 884
CspCI CAANNNNNGTGG 2 cut(s) 898, 933
CviAII CATG 7 cut(s) 16, 490, 649, 713, 748, 1021, 1168
CviQI GTAC 2 cut(s) 118, 483
DdeI CTNAG 4 cut(s) 20, 25, 365, 821
DinI GGCGCC 2 cut(s) 412, 544
DpnI GATC 3 cut(s) 429, 827, 1051
DpnII GATC 3 cut(s) 427, 825, 1049
DraIII CACNNNGTG 1 cut(s) 733
DrdI GACNNNNNNGTC 1 cut(s) 722
DriI GACNNNNNGTC 3 cut(s) 145, 244, 888
DseDI GACNNNNNNGTC 1 cut(s) 722
EaeI YGGCCR 3 cut(s) 96, 601, 681
Eam1104I CTCTTC 1 cut(s) 1119
Eam1105I GACNNNNNGTC 3 cut(s) 145, 244, 888
EarI CTCTTC 1 cut(s) 1119
EciI GGCGGA 1 cut(s) 410
Ecl136II GAGCTC 3 cut(s) 37, 43, 49
Eco130I CCWWGG 2 cut(s) 357, 1005
Eco147I AGGCCT 2 cut(s) 1004, 1135
Eco24I GRGCYC 6 cut(s) 39, 45, 51, 588, 1240, 1309
Eco31I GGTCTC 4 cut(s) 132, 248, 472, 875
Eco47I GGWCC 3 cut(s) 724, 870, 1268
Eco53kI GAGCTC 3 cut(s) 37, 43, 49
Eco57I CTGAAG 2 cut(s) 509, 626
Eco88I CYCGRG 6 cut(s) 38, 44, 435, 1099, 1208, 1239
EcoICRI GAGCTC 3 cut(s) 37, 43, 49
EcoO109I RGGNCCY 3 cut(s) 585, 781, 1268
EcoRII CCWGG 2 cut(s) 783, 791
EcoT14I CCWWGG 2 cut(s) 357, 1005
EcoT38I GRGCYC 6 cut(s) 39, 45, 51, 588, 1240, 1309
EgeI GGCGCC 2 cut(s) 412, 544
EheI GGCGCC 2 cut(s) 412, 544
ErhI CCWWGG 2 cut(s) 357, 1005
Esp3I CGTCTC 2 cut(s) 899, 1137
FaeI CATG 7 cut(s) 19, 493, 652, 716, 751, 1024, 1171
FaqI GGGAC 2 cut(s) 883, 1281
FatI CATG 7 cut(s) 15, 489, 648, 712, 747, 1020, 1167
FauI CCCGC 2 cut(s) 839, 1169
FblI GTMKAC 3 cut(s) 138, 305, 1197
Fnu4HI GCNGC 9 cut(s) 64, 67, 229, 336, 390, 393, 504, 627, 853
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 6 cut(s) 39, 45, 51, 588, 1240, 1309
Fsp4HI GCNGC 9 cut(s) 64, 67, 229, 336, 390, 393, 504, 627, 853
FspBI CTAG 1 cut(s) 536
GlaI GCGC 5 cut(s) 334, 402, 412, 522, 544
GluI GCNGC 9 cut(s) 64, 67, 229, 336, 390, 393, 504, 627, 853
GsaI CCCAGC 1 cut(s) 1284
GsuI CTGGAG 1 cut(s) 344
HaeII RGCGCY 4 cut(s) 404, 414, 524, 546
HhaI GCGC 5 cut(s) 335, 403, 413, 523, 545
Hin1I GRCGYC 4 cut(s) 293, 411, 543, 618
Hin1II CATG 7 cut(s) 19, 493, 652, 716, 751, 1024, 1171
Hin6I GCGC 5 cut(s) 333, 401, 411, 521, 543
HinP1I GCGC 5 cut(s) 333, 401, 411, 521, 543
HincII GTYRAC 2 cut(s) 139, 306
HindII GTYRAC 2 cut(s) 139, 306
HindIII AAGCTT 2 cut(s) 1375, 1400
HphI GGTGA 2 cut(s) 451, 1503
Hpy166II GTNNAC 5 cut(s) 139, 306, 727, 736, 1198
Hpy188I TCNGA 5 cut(s) 159, 253, 578, 958, 1525
Hpy188III TCNNGA 4 cut(s) 644, 840, 1080, 1241
Hpy8I GTNNAC 5 cut(s) 139, 306, 727, 736, 1198
Hpy99I CGWCG 8 cut(s) 140, 295, 298, 301, 310, 616, 894, 979
HpyAV CCTTC 4 cut(s) 323, 533, 790, 1040
HpyCH4III ACNGT 8 cut(s) 259, 482, 706, 874, 975, 1148, 1179, 1482
HpyCH4IV ACGT 4 cut(s) 293, 312, 618, 911
HpyCH4V TGCA 6 cut(s) 66, 376, 778, 1020, 1139, 1228
HpyF10VI GCNNNNNNNGC 5 cut(s) 225, 398, 600, 689, 852
HpyF3I CTNAG 4 cut(s) 20, 25, 365, 821
HpySE526I ACGT 4 cut(s) 293, 312, 618, 911
Hsp92I GRCGYC 4 cut(s) 293, 411, 543, 618
Hsp92II CATG 7 cut(s) 19, 493, 652, 716, 751, 1024, 1171
HspAI GCGC 5 cut(s) 333, 401, 411, 521, 543
KasI GGCGCC 2 cut(s) 410, 542
KflI GGGWCCC 1 cut(s) 1268
Kpn2I TCCGGA 1 cut(s) 1079
KroI GCCGGC 1 cut(s) 1214
KroNI GCCGGC 1 cut(s) 1216
Kzo9I GATC 3 cut(s) 427, 825, 1049
LmnI GCTCC 4 cut(s) 34, 160, 849, 1082
Lsp1109I GCAGC 3 cut(s) 50, 78, 864
LweI GCATC 4 cut(s) 309, 347, 385, 765
MaeI CTAG 1 cut(s) 536
MaeII ACGT 4 cut(s) 293, 312, 618, 911
MaeIII GTNAC 4 cut(s) 9, 281, 313, 1108
MalI GATC 3 cut(s) 429, 827, 1051
MboI GATC 3 cut(s) 427, 825, 1049
MboII GAAGA 2 cut(s) 1133, 1136
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 7 cut(s) 39, 45, 51, 588, 675, 1240, 1309
MluCI AATT 1 cut(s) 1244
Mly113I GGCGCC 2 cut(s) 411, 543
MlyI GAGTC 5 cut(s) 155, 212, 233, 1166, 1196
MmeI TCCRAC 2 cut(s) 930, 1451
MroI TCCGGA 1 cut(s) 1079
MroNI GCCGGC 1 cut(s) 1214
MseI TTAA 3 cut(s) 993, 1359, 1419
MspR9I CCNGG 5 cut(s) 547, 785, 793, 1055, 1235
MvaI CCWGG 2 cut(s) 785, 793
MvnI CGCG 4 cut(s) 335, 594, 626, 841
MwoI GCNNNNNNNGC 5 cut(s) 225, 398, 600, 689, 852
NaeI GCCGGC 1 cut(s) 1216
NarI GGCGCC 2 cut(s) 411, 543
NciI CCSGG 3 cut(s) 547, 1055, 1235
NdeII GATC 3 cut(s) 427, 825, 1049
NgoMIV GCCGGC 1 cut(s) 1214
NlaIII CATG 7 cut(s) 19, 493, 652, 716, 751, 1024, 1171
NlaIV GGNNCC 9 cut(s) 412, 544, 586, 587, 725, 871, 1084, 1269, 1270
NmeAIII GCCGAG 1 cut(s) 1169
NmuCI GTSAC 1 cut(s) 313
NruI TCGCGA 1 cut(s) 841
NspI RCATGY 2 cut(s) 652, 1024
PaeI GCATGC 1 cut(s) 1024
PaeR7I CTCGAG 4 cut(s) 38, 44, 1208, 1239
PceI AGGCCT 2 cut(s) 1004, 1135
PciI ACATGT 1 cut(s) 648
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 302, 314, 1206
PdiI GCCGGC 1 cut(s) 1216
PfeI GAWTC 5 cut(s) 353, 756, 953, 1058, 1520
PflFI GACNNNGTC 1 cut(s) 295
PflMI CCANNNNNTGG 1 cut(s) 733
PfoI TCCNGGA 1 cut(s) 791
PinAI ACCGGT 1 cut(s) 884
PkrI GCNGC 9 cut(s) 65, 68, 230, 337, 391, 394, 505, 628, 854
PleI GAGTC 5 cut(s) 154, 211, 233, 1166, 1196
PluTI GGCGCC 2 cut(s) 414, 546
PpsI GAGTC 5 cut(s) 154, 211, 233, 1166, 1196
PpuMI RGGWCCY 1 cut(s) 1268
PscI ACATGT 1 cut(s) 648
PshAI GACNNNNGTC 1 cut(s) 617
Psp124BI GAGCTC 3 cut(s) 39, 45, 51
Psp5II RGGWCCY 1 cut(s) 1268
Psp6I CCWGG 2 cut(s) 783, 791
PspFI CCCAGC 1 cut(s) 1280
PspGI CCWGG 2 cut(s) 783, 791
PspN4I GGNNCC 9 cut(s) 412, 544, 586, 587, 725, 871, 1084, 1269, 1270
PspOMI GGGCCC 1 cut(s) 584
PspPPI RGGWCCY 1 cut(s) 1268
PspXI VCTCGAGB 3 cut(s) 38, 44, 1208
PstI CTGCAG 2 cut(s) 68, 1141
PstNI CAGNNNCTG 2 cut(s) 784, 1523
PsuI RGATCY 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 295
RruI TCGCGA 1 cut(s) 841
RsaI GTAC 2 cut(s) 119, 484
RsaNI GTAC 2 cut(s) 118, 483
SacI GAGCTC 3 cut(s) 39, 45, 51
SalI GTCGAC 2 cut(s) 137, 304
SaqAI TTAA 3 cut(s) 993, 1359, 1419
SatI GCNGC 9 cut(s) 64, 67, 229, 336, 390, 393, 504, 627, 853
Sau3AI GATC 3 cut(s) 427, 825, 1049
SbfI CCTGCAGG 1 cut(s) 1141
SchI GAGTC 5 cut(s) 155, 212, 233, 1166, 1196
ScrFI CCNGG 5 cut(s) 547, 785, 793, 1055, 1235
SdaI CCTGCAGG 1 cut(s) 1141
SduI GDGCHC 7 cut(s) 39, 45, 51, 588, 675, 1240, 1309
SfaNI GCATC 4 cut(s) 309, 347, 385, 765
SfcI CTRYAG 2 cut(s) 64, 1137
SfiI GGCCNNNNNGGCC 1 cut(s) 689
SfoI GGCGCC 2 cut(s) 412, 544
Sfr274I CTCGAG 4 cut(s) 38, 44, 1208, 1239
SgrAI CRCCGGYG 1 cut(s) 173
SinI GGWCC 3 cut(s) 724, 870, 1268
SlaI CTCGAG 4 cut(s) 38, 44, 1208, 1239
SmlI CTYRAG 5 cut(s) 38, 44, 455, 1208, 1239
SmoI CTYRAG 5 cut(s) 38, 44, 455, 1208, 1239
SphI GCATGC 1 cut(s) 1024
Sse8387I CCTGCAGG 1 cut(s) 1141
Sse9I AATT 1 cut(s) 1244
SseBI AGGCCT 2 cut(s) 1004, 1135
SspDI GGCGCC 2 cut(s) 410, 542
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 3 cut(s) 39, 45, 51
StuI AGGCCT 2 cut(s) 1004, 1135
StyD4I CCNGG 5 cut(s) 545, 783, 791, 1053, 1233
StyI CCWWGG 2 cut(s) 357, 1005
TaaI ACNGT 8 cut(s) 259, 482, 706, 874, 975, 1148, 1179, 1482
TaiI ACGT 4 cut(s) 296, 315, 621, 914
TasI AATT 1 cut(s) 1244
TauI GCSGC 6 cut(s) 231, 338, 392, 395, 506, 629
TfiI GAWTC 5 cut(s) 353, 756, 953, 1058, 1520
Tru1I TTAA 3 cut(s) 993, 1359, 1419
Tru9I TTAA 3 cut(s) 993, 1359, 1419
TscAI CASTG 4 cut(s) 76, 119, 835, 1182
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 3 cut(s) 63, 66, 852
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 2 cut(s) 926, 1156
TspGWI ACGGA 3 cut(s) 323, 629, 1326
TspRI CASTG 4 cut(s) 76, 119, 835, 1182
Tth111I GACNNNGTC 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 733
VpaK11BI GGWCC 3 cut(s) 724, 870, 1268
XceI RCATGY 2 cut(s) 652, 1024
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XhoI CTCGAG 4 cut(s) 38, 44, 1208, 1239
XmiI GTMKAC 3 cut(s) 138, 305, 1197
XspI CTAG 1 cut(s) 536
ZraI GACGTC 2 cut(s) 294, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.