FvH4_2g34211

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002861
Physical Location & Seq
Reverse (-)
25467305 .. 25468453
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34211.t1

Sequence Viewer

Length: 1122 bp
ATGTGCTACTCTGGGGGGTTGGATCATAAAGAAGTTGCTGGAAAGGTAGTCTTATGTAATAATCCCTCCGAGCACCAGACAGACATTGAAGGACAAAAGGAAGAGGTAGAGAGGGCAGGTGCTTATGCTGGAATCTTCATGACGGATCTCTCGTTTTTATATCCAGACGACTACACCATTCCCGCTATAATTCTGCCATTTGCTACAGGTGCCTTGGTAAGAGAACATGCCACACAGGTCAATACAACCAGAGTTAAGAGCATGACATTTGTTCACACCAACTTGGGAACAAAACCAGCACCACAAGTGGCACAGTTCTCTTCAAGAGGGCCTGACTCGATCACTCCAGGTATTCTAAAACCAGACATTCTGGCCCCGGGGGTTGATGTCCTGGCTGCGGTGGCACCTAACAAGCCATCCATGGAACTAAAGAATTACAATTTAGTGACAGATTATGCACTAAAATCAGGGACATCAATGGCAGCACCCCATGCCGCTGGTGTAGCAGCATTGCTTAAAGCAGTTCATCCAGAATGGAGCCCAGCAGCCATCCGATCAGCAATCATGACCACAGCATACGCCATCGACAACACTCACACCACCATAAAAGACCAATGGTCCGGTCTTCCTGCCACACCTCTAGATTTTGGGGCAGGCCACATCAACCCGAATAAGGCCATGAACCCTGGACTAGTCTATGACATGAATGTGCAAGACTACATTGAGTTTCTATGTACCCTGGGGTACAATGACAAGCAGATGAAGGCCGTTATTAGACGAAGCCAATGGACCTGCAGACAACCCCCTACCGAACTAAACTACCCTTCTTTCATGGCCATCATTAACGGCACAGAATCTCCGAGAACCAAAAGCTTTAGCAGGGTTGTGACAAACGTGGGAGATGACACATCAATCTACCAGGCAATTCTACAAGTTCCTAGTGGAATGAGGATAACAGTAGAGCCTAGCACTCTGACATTCACTGCTAAGTATCAGCAGCAAGGTTTTGTTTTGAATGTAGAGATCGACAGTGATGCTCCGAAGGTGATCTATGGTTATCTCAAATGGGTTGATCAACATGATCACATAGTATCAAGCCCTGTAGTTGCCCTCAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

374

Amino Acids

40.66

Weight (kDa)

5.92

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 90 - 200 8e-21 Subtilase family
fn3_6 PF17766 271 - 369 3.7e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 107
Acc36I ACCTGC 2 cut(s) 107, 800
AccB1I GGYRCC 2 cut(s) 209, 403
AciI CCGC 3 cut(s) 183, 398, 495
AclWI GGATC 2 cut(s) 30, 153
AcoI YGGCCR 1 cut(s) 834
AfaI GTAC 2 cut(s) 736, 746
AfiI CCNNNNNNNGG 2 cut(s) 397, 673
AgsI TTSAA 3 cut(s) 89, 324, 1015
AhdI GACNNNNNGTC 1 cut(s) 616
AhlI ACTAGT 1 cut(s) 691
AjnI CCWGG 5 cut(s) 346, 390, 685, 738, 918
AluBI AGCT 1 cut(s) 873
AluI AGCT 1 cut(s) 873
Alw21I GWGCWC 1 cut(s) 75
AlwI GGATC 2 cut(s) 30, 153
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 6 cut(s) 329, 372, 655, 675, 765, 834
ApeKI GCWGC 5 cut(s) 395, 482, 506, 545, 997
AspS9I GGNCC 4 cut(s) 329, 373, 618, 789
AsuC2I CCSGG 2 cut(s) 377, 378
AsuHPI GGTGA 1 cut(s) 1057
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 2 cut(s) 618, 789
BalI TGGCCA 1 cut(s) 836
BanI GGYRCC 2 cut(s) 209, 403
BanII GRGCYC 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 5 cut(s) 382, 494, 518, 557, 1009
BccI CCATC 4 cut(s) 424, 557, 590, 845
BceAI ACGGC 2 cut(s) 752, 862
BcgI CGANNNNNNTGC 2 cut(s) 1016, 1050
BciT130I CCWGG 5 cut(s) 348, 392, 687, 740, 920
BclI TGATCA 2 cut(s) 1072, 1081
BcnI CCSGG 2 cut(s) 377, 378
BcuI ACTAGT 1 cut(s) 691
BfaI CTAG 5 cut(s) 641, 692, 939, 966, 1120
BfmI CTRYAG 3 cut(s) 204, 793, 1101
BfuAI ACCTGC 2 cut(s) 107, 800
BisI GCNGC 6 cut(s) 396, 483, 495, 507, 546, 998
BlsI GCNGC 6 cut(s) 397, 484, 496, 508, 547, 999
Bme1390I CCNGG 7 cut(s) 348, 377, 378, 392, 687, 740, 920
Bme18I GGWCC 2 cut(s) 618, 789
BmeRI GACNNNNNGTC 1 cut(s) 616
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 4 cut(s) 329, 373, 618, 789
BmiI GGNNCC 4 cut(s) 211, 375, 405, 539
BmrFI CCNGG 7 cut(s) 348, 377, 378, 392, 687, 740, 920
BmsI GCATC 1 cut(s) 1024
BpiI GAAGAC 1 cut(s) 617
BpmI CTGGAG 1 cut(s) 330
BpuMI CCSGG 2 cut(s) 377, 378
BsaJI CCNNGG 8 cut(s) 213, 375, 376, 377, 420, 685, 738, 739
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 841, 871
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 673
Bse3DI GCAATG 1 cut(s) 509
BseBI CCWGG 5 cut(s) 348, 392, 687, 740, 920
BseDI CCNNGG 8 cut(s) 213, 375, 376, 377, 420, 685, 738, 739
BseGI GGATG 3 cut(s) 416, 526, 549
BseLI CCNNNNNNNGG 2 cut(s) 397, 673
BseMI GCAATG 1 cut(s) 509
BseXI GCAGC 5 cut(s) 382, 494, 518, 557, 1009
BseYI CCCAGC 1 cut(s) 541
BshFI GGCC 6 cut(s) 331, 374, 657, 677, 767, 836
BshNI GGYRCC 2 cut(s) 209, 403
BsiHKAI GWGCWC 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 2 cut(s) 377, 621
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 2 cut(s) 397, 673
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 6 cut(s) 331, 374, 657, 677, 767, 836
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 2 cut(s) 75, 542
Bsp143I GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
Bsp19I CCATGG 1 cut(s) 420
BspACI CCGC 3 cut(s) 183, 398, 495
BspANI GGCC 6 cut(s) 331, 374, 657, 677, 767, 836
BspHI TCATGA 2 cut(s) 138, 564
BspLI GGNNCC 4 cut(s) 211, 375, 405, 539
BspMAI CTGCAG 1 cut(s) 797
BspMI ACCTGC 2 cut(s) 107, 800
BspPI GGATC 2 cut(s) 30, 153
BspT107I GGYRCC 2 cut(s) 209, 403
BsrDI GCAATG 1 cut(s) 509
BssECI CCNNGG 8 cut(s) 213, 375, 376, 377, 420, 685, 738, 739
BssMI GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
BssT1I CCWWGG 2 cut(s) 213, 420
Bst2UI CCWGG 5 cut(s) 348, 392, 687, 740, 920
Bst4CI ACNGT 3 cut(s) 315, 958, 1031
Bst6I CTCTTC 2 cut(s) 96, 325
BstAPI GCANNNNNTGC 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 655
BstDEI CTNAG 1 cut(s) 987
BstDSI CCRYGG 1 cut(s) 420
BstF5I GGATG 3 cut(s) 416, 526, 549
BstKTI GATC 8 cut(s) 25, 148, 342, 557, 1026, 1050, 1075, 1084
BstMBI GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
BstMWI GCNNNNNNNGC 4 cut(s) 209, 401, 491, 503
BstNI CCWGG 5 cut(s) 348, 392, 687, 740, 920
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 7 cut(s) 346, 375, 376, 390, 685, 738, 918
BstSFI CTRYAG 3 cut(s) 204, 793, 1101
BstV1I GCAGC 5 cut(s) 382, 494, 518, 557, 1009
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 1 cut(s) 145
BsuRI GGCC 6 cut(s) 331, 374, 657, 677, 767, 836
BtgI CCRYGG 1 cut(s) 420
BtsCI GGATG 3 cut(s) 416, 526, 549
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 2 cut(s) 981, 1036
BveI ACCTGC 2 cut(s) 107, 800
Cac8I GCNNGC 1 cut(s) 655
CciI TCATGA 2 cut(s) 138, 564
Cfr13I GGNCC 4 cut(s) 329, 373, 618, 789
Cfr9I CCCGGG 1 cut(s) 376
Csp6I GTAC 2 cut(s) 735, 745
CviQI GTAC 2 cut(s) 735, 745
DdeI CTNAG 1 cut(s) 987
DpnI GATC 8 cut(s) 24, 147, 341, 556, 1025, 1049, 1074, 1083
DpnII GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
DriI GACNNNNNGTC 1 cut(s) 616
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 2 cut(s) 96, 325
Eam1105I GACNNNNNGTC 1 cut(s) 616
EarI CTCTTC 2 cut(s) 96, 325
Eco130I CCWWGG 2 cut(s) 213, 420
Eco24I GRGCYC 1 cut(s) 542
Eco47I GGWCC 2 cut(s) 618, 789
Eco88I CYCGRG 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 329
EcoRII CCWGG 5 cut(s) 346, 390, 685, 738, 918
EcoT14I CCWWGG 2 cut(s) 213, 420
EcoT38I GRGCYC 1 cut(s) 542
ErhI CCWWGG 2 cut(s) 213, 420
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FaqI GGGAC 1 cut(s) 484
FauI CCCGC 1 cut(s) 190
FbaI TGATCA 2 cut(s) 1072, 1081
Fnu4HI GCNGC 6 cut(s) 396, 483, 495, 507, 546, 998
FokI GGATG 3 cut(s) 403, 513, 536
FriOI GRGCYC 1 cut(s) 542
Fsp4HI GCNGC 6 cut(s) 396, 483, 495, 507, 546, 998
FspBI CTAG 5 cut(s) 641, 692, 939, 966, 1120
GluI GCNGC 6 cut(s) 396, 483, 495, 507, 546, 998
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 6 cut(s) 331, 374, 657, 677, 767, 836
HapII CCGG 2 cut(s) 377, 621
HindIII AAGCTT 1 cut(s) 871
HinfI GANTC 3 cut(s) 132, 335, 854
HpaII CCGG 2 cut(s) 377, 621
HphI GGTGA 1 cut(s) 1057
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 5 cut(s) 70, 554, 861, 975, 1041
Hpy188III TCNNGA 6 cut(s) 139, 164, 324, 530, 565, 641
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 4 cut(s) 83, 757, 834, 1036
HpyCH4III ACNGT 3 cut(s) 315, 958, 1031
HpyCH4IV ACGT 1 cut(s) 894
HpyCH4V TGCA 3 cut(s) 458, 712, 795
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 401, 491, 503
HpyF3I CTNAG 1 cut(s) 987
HpySE526I ACGT 1 cut(s) 894
Ksp22I TGATCA 2 cut(s) 1072, 1081
Kzo9I GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
LmnI GCTCC 2 cut(s) 537, 1042
Lsp1109I GCAGC 5 cut(s) 382, 494, 518, 557, 1009
LweI GCATC 1 cut(s) 1024
MaeI CTAG 5 cut(s) 641, 692, 939, 966, 1120
MaeII ACGT 1 cut(s) 894
MaeIII GTNAC 2 cut(s) 445, 886
MalI GATC 8 cut(s) 24, 147, 341, 556, 1025, 1049, 1074, 1083
MboI GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
MboII GAAGA 4 cut(s) 113, 127, 312, 617
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 2 cut(s) 75, 542
MlsI TGGCCA 1 cut(s) 836
MluCI AATT 4 cut(s) 189, 433, 439, 924
MluNI TGGCCA 1 cut(s) 836
MlyI GAGTC 1 cut(s) 329
MnlI CCTC 7 cut(s) 76, 97, 105, 320, 648, 942, 1121
Mox20I TGGCCA 1 cut(s) 836
MscI TGGCCA 1 cut(s) 836
MseI TTAA 3 cut(s) 255, 516, 843
MslI CAYNNNNRTG 1 cut(s) 707
Msp20I TGGCCA 1 cut(s) 836
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 2 cut(s) 377, 621
MspR9I CCNGG 7 cut(s) 348, 377, 378, 392, 687, 740, 920
MvaI CCWGG 5 cut(s) 348, 392, 687, 740, 920
MwoI GCNNNNNNNGC 4 cut(s) 209, 401, 491, 503
NciI CCSGG 2 cut(s) 377, 378
NcoI CCATGG 1 cut(s) 420
NdeII GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
NlaIV GGNNCC 4 cut(s) 211, 375, 405, 539
NmuCI GTSAC 2 cut(s) 445, 886
NspI RCATGY 1 cut(s) 230
PagI TCATGA 2 cut(s) 138, 564
PaqCI CACCTGC 1 cut(s) 107
PasI CCCWGGG 1 cut(s) 739
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PfeI GAWTC 2 cut(s) 132, 854
PkrI GCNGC 6 cut(s) 397, 484, 496, 508, 547, 999
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
Psp6I CCWGG 5 cut(s) 346, 390, 685, 738, 918
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 5 cut(s) 346, 390, 685, 738, 918
PspN4I GGNNCC 4 cut(s) 211, 375, 405, 539
PspPI GGNCC 4 cut(s) 329, 373, 618, 789
PstI CTGCAG 1 cut(s) 797
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 2 cut(s) 736, 746
RsaNI GTAC 2 cut(s) 735, 745
RseI CAYNNNNRTG 1 cut(s) 707
SaqAI TTAA 3 cut(s) 255, 516, 843
SatI GCNGC 6 cut(s) 396, 483, 495, 507, 546, 998
Sau3AI GATC 8 cut(s) 22, 145, 339, 554, 1023, 1047, 1072, 1081
Sau96I GGNCC 4 cut(s) 329, 373, 618, 789
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 7 cut(s) 348, 377, 378, 392, 687, 740, 920
SduI GDGCHC 2 cut(s) 75, 542
SfaNI GCATC 1 cut(s) 1024
SfcI CTRYAG 3 cut(s) 204, 793, 1101
SinI GGWCC 2 cut(s) 618, 789
SmaI CCCGGG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 707
SpeI ACTAGT 1 cut(s) 691
Sse9I AATT 4 cut(s) 189, 433, 439, 924
SsiI CCGC 3 cut(s) 183, 398, 495
SspMI CTAG 5 cut(s) 641, 692, 939, 966, 1120
StyD4I CCNGG 7 cut(s) 346, 375, 376, 390, 685, 738, 918
StyI CCWWGG 2 cut(s) 213, 420
TaaI ACNGT 3 cut(s) 315, 958, 1031
TaiI ACGT 1 cut(s) 897
TaqI TCGA 3 cut(s) 338, 585, 1026
TasI AATT 4 cut(s) 189, 433, 439, 924
TauI GCSGC 1 cut(s) 497
TfiI GAWTC 2 cut(s) 132, 854
Tru1I TTAA 3 cut(s) 255, 516, 843
Tru9I TTAA 3 cut(s) 255, 516, 843
TscAI CASTG 2 cut(s) 988, 1036
TseFI GTSAC 2 cut(s) 445, 886
TseI GCWGC 5 cut(s) 395, 482, 506, 545, 997
Tsp45I GTSAC 2 cut(s) 445, 886
TspDTI ATGAA 6 cut(s) 127, 515, 695, 719, 776, 820
TspGWI ACGGA 1 cut(s) 158
TspMI CCCGGG 1 cut(s) 376
TspRI CASTG 2 cut(s) 988, 1036
VpaK11BI GGWCC 2 cut(s) 618, 789
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 1 cut(s) 230
XmaI CCCGGG 1 cut(s) 376
XspI CTAG 5 cut(s) 641, 692, 939, 966, 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.