Rorug01G0005200

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002861
Physical Location & Seq
Reverse (-)
1039668 .. 1040837
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0005200.1

Sequence Viewer

Length: 1170 bp
ATGGACTTCTCTGTTCTCCACCTACTTGGCCTATACCTTGTCATTGAACCATTTTTTGTAGCTGCTGCCCAAACTGCAAATATCAGCTTGGGCTCGTCTCTCACTGCATCAGACAACTCTCTTGTTTGGTATGCAAATGGAGACAATCCAGCACCAAAAGGATCCAAAGTGGAGCTCACAAAGGACGGTCATTTCAGACTCACTGGCCCTCGGGGAGAAGAAATCTGGCCCCAGCCTGTTATTAGTGGAGTTGCTTATGCTGCCATGCTTGACACAGGTAACTTTGTTCTTGCAAACCAGAATTCAGTACCTTTATGGCAAAGCTTCAAAAACCCTATAGACACCATTTTACCAACCCAAGTGTTGGAAATTGGCGAAAAGCTATCTTCTAGGCAGACTGCTAGCAACTACTCAAAGGGGAAGTTTCAACTCCAATTTAAACCAGCAGGTACGCTTGAGCTGTATCTCATAGCGTTGCCTACAGAGCAAGCATATTCAGCATACTACAAAAGCAATACATCTGATGCAGCTGATAAAATGAACTCCGGCTATCAGCTAGTCTTCAATGAGTCAGGTCATATCAATGTTGTTCAGCGGAATGGAAATATAGTAACCCTCACAAACAAAACCGCATCCCCACCAAGAGATTACTATTACAGAGCAACACTAGATTATGATGGGCTTTTTACCCTATATGCTTATCCAAAAAATGGGAGCGGGACATCCTGGTCCTCTTTGTGGTCTATTCCAGAAAACATTTGCTTCGATTCAACTGGAAACGAGGGCGGCGGGCCATGTGGATATAACAGTTACTGCAGACTTGATGCAAACACAAGGCCAATTTGTGAATGCCTTCCTGGCTTTTCTGCTTTAGATCCTGAATATAAGTTGCGCGGCTGCAAACAAAACAGGATGCACAGTTGTGAACTAGGTGATTCAAAGCCACAAGATGTATATGTGATGCATGAGCTACCAAATACTTTCTGGCCCAGTTCTGCAAACTATGAAGAGTTGCAGCCACTCAATGAAGATGATTGTAGCAGATTTTGCCTCGATGATTGTAACTGTGTGGTAGCTGTTATCAAACTGTGTGGTAACATGTATGAAAACTGGGTCGGGTTTAAAAGTCCATGTACTTATGTTGGGTTTTATAAACGTTGGCCACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

389

Amino Acids

43.25

Weight (kDa)

5.15

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 41 - 129 2.3e-13 D-mannose binding lectin
S_locus_glycop PF00954 200 - 289 3e-06 S-locus glycoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1152
AasI GACNNNNNNGTC 1 cut(s) 727
Acc36I ACCTGC 1 cut(s) 437
AccB7I CCANNNNNTGG 2 cut(s) 364, 710
AccBSI CCGCTC 1 cut(s) 717
AccII CGCG 1 cut(s) 894
AciI CCGC 6 cut(s) 595, 630, 717, 786, 789, 894
AclI AACGTT 1 cut(s) 1156
AclWI GGATC 3 cut(s) 156, 169, 869
AcoI YGGCCR 1 cut(s) 1160
AcsI RAATTY 1 cut(s) 301
AfaI GTAC 3 cut(s) 309, 451, 1135
AfiI CCNNNNNNNGG 3 cut(s) 364, 710, 738
AflIII ACRYGT 1 cut(s) 1098
AgsI TTSAA 6 cut(s) 47, 328, 428, 565, 771, 939
AjnI CCWGG 2 cut(s) 725, 856
AleI CACNNNNGTG 1 cut(s) 921
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 2 cut(s) 102, 135
AlwI GGATC 3 cut(s) 156, 169, 869
AlwNI CAGNNNCTG 1 cut(s) 813
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 7 cut(s) 28, 205, 227, 791, 836, 986, 1160
ApeKI GCWGC 6 cut(s) 62, 65, 260, 527, 897, 1015
ApoI RAATTY 1 cut(s) 301
Asp700I GAANNNNTTC 1 cut(s) 852
AspLEI GCGC 1 cut(s) 894
AspS9I GGNCC 5 cut(s) 206, 228, 729, 791, 987
AsuHPI GGTGA 1 cut(s) 944
AsuNHI GCTAGC 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 1 cut(s) 729
BalI TGGCCA 1 cut(s) 1162
BamHI GGATCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 95, 177
BbsI GAAGAC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 6 cut(s) 49, 52, 247, 539, 884, 1027
BccI CCATC 1 cut(s) 671
BciT130I CCWGG 2 cut(s) 727, 858
BcoDI GTCTC 2 cut(s) 102, 135
BfaI CTAG 5 cut(s) 390, 402, 557, 668, 929
BfmI CTRYAG 3 cut(s) 336, 480, 814
BfuAI ACCTGC 1 cut(s) 437
BglI GCCNNNNNGGC 1 cut(s) 858
BisI GCNGC 8 cut(s) 63, 66, 261, 528, 787, 895, 898, 1016
BlsI GCNGC 8 cut(s) 64, 67, 262, 529, 788, 896, 899, 1017
Bme1390I CCNGG 2 cut(s) 727, 858
Bme18I GGWCC 1 cut(s) 729
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 5 cut(s) 206, 228, 729, 791, 987
BmiI GGNNCC 2 cut(s) 163, 230
BmrFI CCNGG 2 cut(s) 727, 858
BmrI ACTGGG 2 cut(s) 984, 1120
BmsI GCATC 6 cut(s) 116, 514, 641, 814, 903, 951
BmtI GCTAGC 1 cut(s) 405
BmuI ACTGGG 2 cut(s) 984, 1120
BpiI GAAGAC 1 cut(s) 553
BpuEI CTTGAG 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 209
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 710, 738
Bse1I ACTGG 4 cut(s) 208, 778, 990, 1115
BseBI CCWGG 2 cut(s) 727, 858
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 3 cut(s) 632, 722, 918
BseLI CCNNNNNNNGG 3 cut(s) 364, 710, 738
BseNI ACTGG 4 cut(s) 208, 778, 990, 1115
BseXI GCAGC 6 cut(s) 49, 52, 247, 539, 884, 1027
BseYI CCCAGC 1 cut(s) 231
Bsh1236I CGCG 1 cut(s) 894
BshFI GGCC 7 cut(s) 30, 207, 229, 793, 838, 988, 1162
BsiHKAI GWGCWC 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 1 cut(s) 546
BslFI GGGAC 1 cut(s) 733
BslI CCNNNNNNNGG 3 cut(s) 364, 710, 738
BsmAI GTCTC 2 cut(s) 102, 135
BsmBI CGTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 733
BsmI GAATGC 1 cut(s) 854
BsnI GGCC 7 cut(s) 30, 207, 229, 793, 838, 988, 1162
BsoBI CYCGRG 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 95, 177
Bsp143I GATC 2 cut(s) 161, 874
BspACI CCGC 6 cut(s) 595, 630, 717, 786, 789, 894
BspANI GGCC 7 cut(s) 30, 207, 229, 793, 838, 988, 1162
BspFNI CGCG 1 cut(s) 894
BspLI GGNNCC 2 cut(s) 163, 230
BspMAI CTGCAG 1 cut(s) 818
BspMI ACCTGC 1 cut(s) 437
BspOI GCTAGC 1 cut(s) 405
BspPI GGATC 3 cut(s) 156, 169, 869
BsrBI CCGCTC 1 cut(s) 717
BsrI ACTGG 4 cut(s) 208, 778, 990, 1115
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 2 cut(s) 161, 874
Bst2UI CCWGG 2 cut(s) 727, 858
Bst4CI ACNGT 5 cut(s) 188, 809, 920, 1067, 1089
Bst6I CTCTTC 1 cut(s) 1002
BstAPI GCANNNNNTGC 1 cut(s) 1047
BstC8I GCNNGC 3 cut(s) 403, 489, 791
BstF5I GGATG 3 cut(s) 632, 722, 918
BstFNI CGCG 1 cut(s) 894
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 2 cut(s) 164, 877
BstMAI GTCTC 2 cut(s) 102, 135
BstMBI GATC 2 cut(s) 161, 874
BstMWI GCNNNNNNNGC 6 cut(s) 74, 260, 484, 497, 858, 1047
BstNI CCWGG 2 cut(s) 727, 858
BstNSI RCATGY 1 cut(s) 1102
BstSCI CCNGG 2 cut(s) 725, 856
BstSFI CTRYAG 3 cut(s) 336, 480, 814
BstUI CGCG 1 cut(s) 894
BstV1I GCAGC 6 cut(s) 49, 52, 247, 539, 884, 1027
BstV2I GAAGAC 1 cut(s) 553
BstX2I RGATCY 2 cut(s) 161, 874
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 2 cut(s) 161, 874
BsuRI GGCC 7 cut(s) 30, 207, 229, 793, 838, 988, 1162
BtsCI GGATG 3 cut(s) 632, 722, 918
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 102, 201
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 3 cut(s) 403, 489, 791
CaiI CAGNNNCTG 1 cut(s) 813
CfoI GCGC 1 cut(s) 894
Cfr13I GGNCC 5 cut(s) 206, 228, 729, 791, 987
Csp6I GTAC 3 cut(s) 308, 450, 1134
CspCI CAANNNNNGTGG 2 cut(s) 1072, 1107
CviAII CATG 5 cut(s) 265, 795, 965, 1099, 1131
CviQI GTAC 3 cut(s) 308, 450, 1134
DpnI GATC 2 cut(s) 163, 876
DpnII GATC 2 cut(s) 161, 874
DraI TTTAAA 2 cut(s) 439, 1123
DrdI GACNNNNNNGTC 1 cut(s) 727
DseDI GACNNNNNNGTC 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 1160
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
Ecl136II GAGCTC 1 cut(s) 175
Eco24I GRGCYC 2 cut(s) 95, 177
Eco47I GGWCC 1 cut(s) 729
Eco53kI GAGCTC 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 175
EcoRI GAATTC 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 725, 856
EcoT22I ATGCAT 1 cut(s) 966
EcoT38I GRGCYC 2 cut(s) 95, 177
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 5 cut(s) 268, 798, 968, 1102, 1134
FaqI GGGAC 1 cut(s) 733
FatI CATG 5 cut(s) 264, 794, 964, 1098, 1130
FauI CCCGC 2 cut(s) 710, 782
Fnu4HI GCNGC 8 cut(s) 63, 66, 261, 528, 787, 895, 898, 1016
FokI GGATG 3 cut(s) 619, 709, 925
FriOI GRGCYC 2 cut(s) 95, 177
Fsp4HI GCNGC 8 cut(s) 63, 66, 261, 528, 787, 895, 898, 1016
FspBI CTAG 5 cut(s) 390, 402, 557, 668, 929
GlaI GCGC 1 cut(s) 893
GluI GCNGC 8 cut(s) 63, 66, 261, 528, 787, 895, 898, 1016
GsaI CCCAGC 1 cut(s) 235
HaeIII GGCC 7 cut(s) 30, 207, 229, 793, 838, 988, 1162
HapII CCGG 1 cut(s) 546
HhaI GCGC 1 cut(s) 894
Hin1II CATG 5 cut(s) 268, 798, 968, 1102, 1134
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 4 cut(s) 198, 569, 767, 935
HpaII CCGG 1 cut(s) 546
HphI GGTGA 1 cut(s) 944
Hpy166II GTNNAC 1 cut(s) 926
Hpy188I TCNGA 3 cut(s) 112, 197, 523
Hpy188III TCNNGA 2 cut(s) 749, 878
Hpy8I GTNNAC 1 cut(s) 926
HpyAV CCTTC 1 cut(s) 863
HpyCH4III ACNGT 5 cut(s) 188, 809, 920, 1067, 1089
HpyCH4IV ACGT 1 cut(s) 1156
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 260, 484, 497, 858, 1047
HpySE526I ACGT 1 cut(s) 1156
Hsp92II CATG 5 cut(s) 268, 798, 968, 1102, 1134
HspAI GCGC 1 cut(s) 892
Kzo9I GATC 2 cut(s) 161, 874
LmnI GCTCC 2 cut(s) 172, 714
Lsp1109I GCAGC 6 cut(s) 49, 52, 247, 539, 884, 1027
LweI GCATC 6 cut(s) 116, 514, 641, 814, 903, 951
MaeI CTAG 5 cut(s) 390, 402, 557, 668, 929
MaeII ACGT 1 cut(s) 1156
MaeIII GTNAC 5 cut(s) 278, 610, 809, 1061, 1094
MalI GATC 2 cut(s) 163, 876
MbiI CCGCTC 1 cut(s) 717
MboI GATC 2 cut(s) 161, 874
MboII GAAGA 5 cut(s) 230, 378, 553, 1019, 1040
MflI RGATCY 2 cut(s) 161, 874
MhlI GDGCHC 2 cut(s) 95, 177
MlsI TGGCCA 1 cut(s) 1162
MluCI AATT 4 cut(s) 301, 369, 434, 840
MluNI TGGCCA 1 cut(s) 1162
MlyI GAGTC 2 cut(s) 192, 578
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 5 cut(s) 219, 626, 742, 775, 1061
Mox20I TGGCCA 1 cut(s) 1162
Mph1103I ATGCAT 1 cut(s) 966
MroXI GAANNNNTTC 1 cut(s) 852
MscI TGGCCA 1 cut(s) 1162
MseI TTAA 3 cut(s) 438, 1122, 1168
MslI CAYNNNNRTG 2 cut(s) 582, 921
Msp20I TGGCCA 1 cut(s) 1162
MspA1I CMGCKG 2 cut(s) 530, 595
MspI CCGG 1 cut(s) 546
MspR9I CCNGG 2 cut(s) 727, 858
Mva1269I GAATGC 1 cut(s) 854
MvaI CCWGG 2 cut(s) 727, 858
MvnI CGCG 1 cut(s) 894
MwoI GCNNNNNNNGC 6 cut(s) 74, 260, 484, 497, 858, 1047
NdeII GATC 2 cut(s) 161, 874
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 5 cut(s) 268, 798, 968, 1102, 1134
NlaIV GGNNCC 2 cut(s) 163, 230
NsiI ATGCAT 1 cut(s) 966
NspI RCATGY 1 cut(s) 1102
OliI CACNNNNGTG 1 cut(s) 921
PciI ACATGT 1 cut(s) 1098
PctI GAATGC 1 cut(s) 854
PdmI GAANNNNTTC 1 cut(s) 852
PfeI GAWTC 2 cut(s) 767, 935
PflMI CCANNNNNTGG 2 cut(s) 364, 710
PkrI GCNGC 8 cut(s) 64, 67, 262, 529, 788, 896, 899, 1017
PleI GAGTC 2 cut(s) 192, 577
PpsI GAGTC 2 cut(s) 192, 577
PscI ACATGT 1 cut(s) 1098
PsiI TTATAA 1 cut(s) 1152
Psp124BI GAGCTC 1 cut(s) 177
Psp1406I AACGTT 1 cut(s) 1156
Psp6I CCWGG 2 cut(s) 725, 856
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 2 cut(s) 725, 856
PspN4I GGNNCC 2 cut(s) 163, 230
PspPI GGNCC 5 cut(s) 206, 228, 729, 791, 987
PstI CTGCAG 1 cut(s) 818
PstNI CAGNNNCTG 1 cut(s) 813
PsuI RGATCY 2 cut(s) 161, 874
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 309, 451, 1135
RsaNI GTAC 3 cut(s) 308, 450, 1134
RseI CAYNNNNRTG 2 cut(s) 582, 921
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 438, 1122, 1168
SatI GCNGC 8 cut(s) 63, 66, 261, 528, 787, 895, 898, 1016
Sau3AI GATC 2 cut(s) 161, 874
Sau96I GGNCC 5 cut(s) 206, 228, 729, 791, 987
SchI GAGTC 2 cut(s) 192, 578
ScrFI CCNGG 2 cut(s) 727, 858
SduI GDGCHC 2 cut(s) 95, 177
SfaNI GCATC 6 cut(s) 116, 514, 641, 814, 903, 951
SfcI CTRYAG 3 cut(s) 336, 480, 814
SinI GGWCC 1 cut(s) 729
SmiMI CAYNNNNRTG 2 cut(s) 582, 921
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 4 cut(s) 301, 369, 434, 840
SsiI CCGC 6 cut(s) 595, 630, 717, 786, 789, 894
SspMI CTAG 5 cut(s) 390, 402, 557, 668, 929
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 725, 856
TaaI ACNGT 5 cut(s) 188, 809, 920, 1067, 1089
TaiI ACGT 1 cut(s) 1159
TaqI TCGA 2 cut(s) 765, 1053
TasI AATT 4 cut(s) 301, 369, 434, 840
TatI WGTACW 1 cut(s) 1133
TauI GCSGC 2 cut(s) 789, 897
TfiI GAWTC 2 cut(s) 767, 935
Tru1I TTAA 3 cut(s) 438, 1122, 1168
Tru9I TTAA 3 cut(s) 438, 1122, 1168
TscAI CASTG 2 cut(s) 109, 208
TseI GCWGC 6 cut(s) 62, 65, 260, 527, 897, 1015
TspDTI ATGAA 4 cut(s) 554, 1020, 1041, 1119
TspRI CASTG 2 cut(s) 109, 208
Van91I CCANNNNNTGG 2 cut(s) 364, 710
VpaK11BI GGWCC 1 cut(s) 729
XapI RAATTY 1 cut(s) 301
XceI RCATGY 1 cut(s) 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 981
XmnI GAANNNNTTC 1 cut(s) 852
XspI CTAG 5 cut(s) 390, 402, 557, 668, 929
Zsp2I ATGCAT 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.